Sed0027297 (gene) Chayote v1

Overview
NameSed0027297
Typegene
OrganismSechium edule (Chayote v1)
DescriptionMitogen-activated protein kinase kinase kinase
LocationLG09: 38018301 .. 38019739 (+)
RNA-Seq ExpressionSed0027297
SyntenySed0027297
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAGATCATCAAATTCAATGGAAGACGATTCCAAAGACGAACAAGAAATCGCTGCTGCTTCGTCTTCTTCGAACCATGGACTACATTGGATAAGAGGGTCGGTGATCGGGAAAGGAGGTTTCGCATCGGTTTTCATGGCTTCTCTTAAGCCATCTGTGGCTGAACGCAGCATTTACCCTTCGGTAATGGCCGTCAAGTCTGCCGAGGTTTCCGATTCTGGAACCCTACAAAAGGAGAAATTAGCTTACGACAACTTACATTCTTGCGAGTCTTTGATCCAATGCTATGGTGAAGAGACCACTTTCAATGATCATGGTCAGATGATTTATAATTTGCTGCTCGAGTTCGCAGAAGGAGGCGACCTAGCTCATCGAATCAAGGGTACAAGTAATTTGGAGACTCAAACTTTTGACTTCTTGATAATATCAATGTTCTCTTTGGATTTTTGTATTTCAATAACATCATTTGGGGTTTGAGTGCTCGAAAGTCTTACTTCTTAATCGATTATAAATGTCAGTTATTATTTAGTTAAGTTAACTTTGATAATTTAATCTCTTTTTTTTTAGTTCAACAACTCTTAATTGAGCTTAAGGCAGTGTTATTTTTATTTTAAAGTTTTACTTAATATTAAATTTTTAAAAAAAATTAAAAAAAGACTGCGGTGACTTTGCCCTAAATTACTGCCGAGGTTTTACAAAATTTTGTAGGGTTTTTAAATATTTATTATTACATTTTTTTTTTTCTCCTACTAAATTATTTTTAATGGTAATTTGAATATATAGGTGGAAAGGGTTTACCAGAAATTGAGGTTCGAAAATACACAAGATCAATAATCAAAGGGTTAATTCATATTCACAAATCCAAATATGTTCATTGTGATTTAAAGCCAGCAAATATCCTATTGGTGCCGAGGAATTCATCCAAAGGTCAACGATTCACGGCAAAGATCGGCGATTTAGGGTCAGCGAGGAGAATAAACAAGACAAAGCCATTCTATAGTTTGGGAGGGACAGTGATGTACATGGCGCCAGAGACATTGATTCACAACGTGCAAGAGTCGCCGAGCGATATTTGGGCACTCGGGTGCCTCGTGCTCGAAATGCTCACGGGAAACCGCCCGTGGACCGCTGCAGATAGGGCTGGGATTGCTAAACAGATCGTAGAGAAGCATAGAATGCCGAAAATTCCAGTCGAGGTTTCGGAGGAGGCACGTTGGTTTTTGAAGTGTTGCTTTGTGAGGAAGCCAAAGTTGAGGCCTACTGCAGAGATGCTGATGAATTTTCCGTTCGTGGTGGCGGCGACGGGTGAAGAAGATGATGATTTTGTGGCGGCTCCAACATCTGTGGAGATCAACAGGAGATCTTCTAAGAGGCTTAGGGTTTCTTTTAAAATTGTGAGAAAAAGTAATATTGCTACAAAAAATAATGATATCTAA

mRNA sequence

ATGAAGAGATCATCAAATTCAATGGAAGACGATTCCAAAGACGAACAAGAAATCGCTGCTGCTTCGTCTTCTTCGAACCATGGACTACATTGGATAAGAGGGTCGGTGATCGGGAAAGGAGGTTTCGCATCGGTTTTCATGGCTTCTCTTAAGCCATCTGTGGCTGAACGCAGCATTTACCCTTCGGTAATGGCCGTCAAGTCTGCCGAGGTTTCCGATTCTGGAACCCTACAAAAGGAGAAATTAGCTTACGACAACTTACATTCTTGCGAGTCTTTGATCCAATGCTATGGTGAAGAGACCACTTTCAATGATCATGGTCAGATGATTTATAATTTGCTGCTCGAGTTCGCAGAAGGAGGCGACCTAGCTCATCGAATCAAGGGTACAAGTGGAAAGGGTTTACCAGAAATTGAGGTTCGAAAATACACAAGATCAATAATCAAAGGGTTAATTCATATTCACAAATCCAAATATGTTCATTGTGATTTAAAGCCAGCAAATATCCTATTGGTGCCGAGGAATTCATCCAAAGGTCAACGATTCACGGCAAAGATCGGCGATTTAGGGTCAGCGAGGAGAATAAACAAGACAAAGCCATTCTATAGTTTGGGAGGGACAGTGATGTACATGGCGCCAGAGACATTGATTCACAACGTGCAAGAGTCGCCGAGCGATATTTGGGCACTCGGGTGCCTCGTGCTCGAAATGCTCACGGGAAACCGCCCGTGGACCGCTGCAGATAGGGCTGGGATTGCTAAACAGATCGTAGAGAAGCATAGAATGCCGAAAATTCCAGTCGAGGTTTCGGAGGAGGCACGTTGGTTTTTGAAGTGTTGCTTTGTGAGGAAGCCAAAGTTGAGGCCTACTGCAGAGATGCTGATGAATTTTCCGTTCGTGGTGGCGGCGACGGGTGAAGAAGATGATGATTTTGTGGCGGCTCCAACATCTGTGGAGATCAACAGGAGATCTTCTAAGAGGCTTAGGGTTTCTTTTAAAATTGTGAGAAAAAGTAATATTGCTACAAAAAATAATGATATCTAA

Coding sequence (CDS)

ATGAAGAGATCATCAAATTCAATGGAAGACGATTCCAAAGACGAACAAGAAATCGCTGCTGCTTCGTCTTCTTCGAACCATGGACTACATTGGATAAGAGGGTCGGTGATCGGGAAAGGAGGTTTCGCATCGGTTTTCATGGCTTCTCTTAAGCCATCTGTGGCTGAACGCAGCATTTACCCTTCGGTAATGGCCGTCAAGTCTGCCGAGGTTTCCGATTCTGGAACCCTACAAAAGGAGAAATTAGCTTACGACAACTTACATTCTTGCGAGTCTTTGATCCAATGCTATGGTGAAGAGACCACTTTCAATGATCATGGTCAGATGATTTATAATTTGCTGCTCGAGTTCGCAGAAGGAGGCGACCTAGCTCATCGAATCAAGGGTACAAGTGGAAAGGGTTTACCAGAAATTGAGGTTCGAAAATACACAAGATCAATAATCAAAGGGTTAATTCATATTCACAAATCCAAATATGTTCATTGTGATTTAAAGCCAGCAAATATCCTATTGGTGCCGAGGAATTCATCCAAAGGTCAACGATTCACGGCAAAGATCGGCGATTTAGGGTCAGCGAGGAGAATAAACAAGACAAAGCCATTCTATAGTTTGGGAGGGACAGTGATGTACATGGCGCCAGAGACATTGATTCACAACGTGCAAGAGTCGCCGAGCGATATTTGGGCACTCGGGTGCCTCGTGCTCGAAATGCTCACGGGAAACCGCCCGTGGACCGCTGCAGATAGGGCTGGGATTGCTAAACAGATCGTAGAGAAGCATAGAATGCCGAAAATTCCAGTCGAGGTTTCGGAGGAGGCACGTTGGTTTTTGAAGTGTTGCTTTGTGAGGAAGCCAAAGTTGAGGCCTACTGCAGAGATGCTGATGAATTTTCCGTTCGTGGTGGCGGCGACGGGTGAAGAAGATGATGATTTTGTGGCGGCTCCAACATCTGTGGAGATCAACAGGAGATCTTCTAAGAGGCTTAGGGTTTCTTTTAAAATTGTGAGAAAAAGTAATATTGCTACAAAAAATAATGATATCTAA

Protein sequence

MKRSSNSMEDDSKDEQEIAAASSSSNHGLHWIRGSVIGKGGFASVFMASLKPSVAERSIYPSVMAVKSAEVSDSGTLQKEKLAYDNLHSCESLIQCYGEETTFNDHGQMIYNLLLEFAEGGDLAHRIKGTSGKGLPEIEVRKYTRSIIKGLIHIHKSKYVHCDLKPANILLVPRNSSKGQRFTAKIGDLGSARRINKTKPFYSLGGTVMYMAPETLIHNVQESPSDIWALGCLVLEMLTGNRPWTAADRAGIAKQIVEKHRMPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFVVAATGEEDDDFVAAPTSVEINRRSSKRLRVSFKIVRKSNIATKNNDI
Homology
BLAST of Sed0027297 vs. NCBI nr
Match: KAA0058687.1 (mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa] >TYK10493.1 mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 382.1 bits (980), Expect = 5.1e-102
Identity = 211/334 (63.17%), Postives = 244/334 (73.05%), Query Frame = 0

Query: 1   MKRSSNSMEDDSKDEQEIAAASSSSNHGLHWIRGSVIGKGGFASVFMASLKPSVA-ERSI 60
           MKR  NSM ++   E E+   SS  N+G+ W RG +IGKG F SVF+ASLKP ++ + SI
Sbjct: 1   MKR--NSMLEEL--EVEVEDGSSEFNNGIQWKRGRLIGKGSFGSVFLASLKPHISIKYSI 60

Query: 61  YPSVMAVKSAEVSDSGTLQKEKLAYDNLHSCESLIQCYGEETTFNDHGQMIYNLLLEFAE 120
           +PSVMAVKSAE+S S TLQKEK  YDNL  C SLIQC+GEE T + +G MIYNLLLE A 
Sbjct: 61  FPSVMAVKSAEISVSETLQKEKQNYDNLKGCSSLIQCFGEEITTDHNGHMIYNLLLEVAT 120

Query: 121 GGDLAHRIKGTSGKGLPEIEVRKYTRSIIKGLIHIHKSKYVHCDLKPANILLVPRNSSKG 180
           GG LAH IK T GKGL E  VR YT+SIIKGLIHIH+S+YVHCDLKPANILL+P+N++  
Sbjct: 121 GGTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLIPKNNTTT 180

Query: 181 QR-FTAKIGDLGSARRINKTKPFYSLGGTVMYMAPETLIHNVQESPSDIWALGCLVLEML 240
            R F AKI DLG ARR +KTK  Y LGGT  YMAPETLI  VQES SDIWALGC+VLEML
Sbjct: 181 NRQFIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCIVLEML 240

Query: 241 TGNRPWTAADRAGIAKQIVEK-HRMPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNF 300
           TGNR W A D+ GI K++ E  H MPKIP  +S EA  FLK C VRKP+ R TAEMLMN 
Sbjct: 241 TGNRAWAATDKVGIVKEMTENLHGMPKIPDGLSVEATGFLKNCLVRKPEFRFTAEMLMNV 300

Query: 301 PFVVAATGEEDD-DFVAAPTSVEINRRSSKRLRV 331
           PFV AA  +E D + + APTSV    R  KR R+
Sbjct: 301 PFVAAAVDQEQDFNTMKAPTSVTKWPRQFKRQRI 330

BLAST of Sed0027297 vs. NCBI nr
Match: XP_008461431.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 381.7 bits (979), Expect = 6.7e-102
Identity = 211/334 (63.17%), Postives = 244/334 (73.05%), Query Frame = 0

Query: 1   MKRSSNSMEDDSKDEQEIAAASSSSNHGLHWIRGSVIGKGGFASVFMASLKPSVA-ERSI 60
           MKR  NSM ++   E E+   SS  N+G+ W RG +IGKG F SVF+ASLKP ++ + SI
Sbjct: 1   MKR--NSMLEEL--EVEVEDGSSEFNNGIQWKRGRLIGKGSFGSVFLASLKPHISIKYSI 60

Query: 61  YPSVMAVKSAEVSDSGTLQKEKLAYDNLHSCESLIQCYGEETTFNDHGQMIYNLLLEFAE 120
           +PSVMAVKSAE+S S TLQKEK  YDNL  C SLIQC+GEE T + +G MIYNLLLE A 
Sbjct: 61  FPSVMAVKSAEISVSETLQKEKQNYDNLKGCSSLIQCFGEEITTDHNGHMIYNLLLEVAT 120

Query: 121 GGDLAHRIKGTSGKGLPEIEVRKYTRSIIKGLIHIHKSKYVHCDLKPANILLVPRNSSKG 180
           GG LAH IK T GKGL E  VR YT+SIIKGLIHIH+S+YVHCDLKPANILL+P+N++  
Sbjct: 121 GGTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLIPKNNTTT 180

Query: 181 QR-FTAKIGDLGSARRINKTKPFYSLGGTVMYMAPETLIHNVQESPSDIWALGCLVLEML 240
            R F AKI DLG ARR +KTK  Y LGGT  YMAPETLI  VQES SDIWALGC+VLEML
Sbjct: 181 NRQFIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEML 240

Query: 241 TGNRPWTAADRAGIAKQIVEK-HRMPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNF 300
           TGNR W A D+ GI K++ E  H MPKIP  +S EA  FLK C VRKP+ R TAEMLMN 
Sbjct: 241 TGNRAWAATDKVGIVKEMTENLHGMPKIPDGLSVEATGFLKNCLVRKPEFRFTAEMLMNV 300

Query: 301 PFVVAATGEEDD-DFVAAPTSVEINRRSSKRLRV 331
           PFV AA  +E D + + APTSV    R  KR R+
Sbjct: 301 PFVAAAVDQEQDFNTMKAPTSVTKWPRQFKRQRI 330

BLAST of Sed0027297 vs. NCBI nr
Match: KAG7014197.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 380.9 bits (977), Expect = 1.1e-101
Identity = 204/336 (60.71%), Postives = 234/336 (69.64%), Query Frame = 0

Query: 1   MKRSSNSMEDDSKDEQEIAAASSSSNHGLHWIRGSVIGKGGFASVFMASLKPSVAERSIY 60
           MKR + S+   S      ++     N G+ W RG +IGKG F SVFMASLK S AE SI+
Sbjct: 1   MKRKNESIASSSSSSSSSSSCCCCCNDGVQWERGRLIGKGSFGSVFMASLKRSTAEFSIF 60

Query: 61  PSVMAVKSAEVSDSGTLQKEKLAYDNLHSCESLIQCYGEETTFNDHGQMIYNLLLEFAEG 120
           P VMAVKSAEVS S +LQKEK  YDNL  C SLIQC+GEE T + +G MIYNLLLE A G
Sbjct: 61  PPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRNGPMIYNLLLEIALG 120

Query: 121 GDLAHRIKGTSGKGLPEIEVRKYTRSIIKGLIHIHKSKYVHCDLKPANILLVPRNSSKGQ 180
           G LAHRI  T GKGL E EV +YT++IIKGLIHIH+  YVHCDLKPANILL+P N++KG 
Sbjct: 121 GSLAHRINNTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANILLLPTNTAKGG 180

Query: 181 RFTAKIGDLGSARRINKTKPFYSLGGTVMYMAPETLIHNVQESPSDIWALGCLVLEMLTG 240
           R  AKI DLG ARR ++T+     GGT+ YMAPETLIH VQESPSDIWALGCLVLEMLTG
Sbjct: 181 RLVAKICDLGLARRTSETRHGNCFGGTLSYMAPETLIHRVQESPSDIWALGCLVLEMLTG 240

Query: 241 NRPWTAADRAGIAKQIVEKHRMPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFV 300
           NRPW A D AGI K I E   MP+IP  VS+EA  FLK CFVR P+ R TAEMLM  PFV
Sbjct: 241 NRPWAATDEAGIVKSITENDGMPEIPEGVSKEASGFLKNCFVRNPEYRFTAEMLMTVPFV 300

Query: 301 VA---------ATGEEDDDFVAAPTSVEINRRSSKR 328
            A            EE++DF+ A T V    RS ++
Sbjct: 301 AAEEEEEEEEEEEEEEEEDFMRALTFVRKRSRSCRK 336

BLAST of Sed0027297 vs. NCBI nr
Match: XP_022154776.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 375.6 bits (963), Expect = 4.8e-100
Identity = 204/334 (61.08%), Postives = 243/334 (72.75%), Query Frame = 0

Query: 8   MEDDSKDEQEIAAASSSSNHGLHWIRGSVIGKGGFASVFMASLKPSVAERSIYPSVMAVK 67
           M+  S +  EI    +  NHG+ W RG +IGKG F SVFMASL+  +++ SI+P VMAVK
Sbjct: 1   MKRKSTENDEI--VPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVK 60

Query: 68  SAEVSDSGTLQKEKLAYDNLHSCESLIQCYGEETTFNDHGQMIYNLLLEFAEGGDLAHRI 127
           SAEVSDS TLQKEK  YDNL  C+SLI+C+GEE T +++GQMIYNLLLEFA  G LA +I
Sbjct: 61  SAEVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQI 120

Query: 128 KGTSGKGLPEIEVRKYTRSIIKGLIHIHKSKYVHCDLKPANILLVPRNSSKGQRFTAKIG 187
           K T GKGL E EV  YTRSIIKGLIHIHK++YVHCDLKP NILLVPRNS+ G+R  AKIG
Sbjct: 121 KNTGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIG 180

Query: 188 DLGSARRINKTKPFYSLGGTVMYMAPETLIHNVQESPSDIWALGCLVLEMLTGNRPWTAA 247
           DLG A+R +KTK    L GTV YMAPETL+++VQESPSDIWALGC+VLEMLTGNRPW+  
Sbjct: 181 DLGLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGG 240

Query: 248 ----DRAGIAKQIVEKHRMPKIP--VEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFVV 307
               D   I ++  EK+ +P IP    +S EAR FLK CFVRKP+ R TAEMLM  PF+ 
Sbjct: 241 GGGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLA 300

Query: 308 AATGEEDDDFVAAPTSVEINRRSSKRLRVSFKIV 336
               EE++  V AP  VE  RR SKR R+  + V
Sbjct: 301 EDEEEEEESVVGAPRFVEKARR-SKRQRIPIQAV 331

BLAST of Sed0027297 vs. NCBI nr
Match: XP_008461430.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 373.6 bits (958), Expect = 1.8e-99
Identity = 198/321 (61.68%), Postives = 234/321 (72.90%), Query Frame = 0

Query: 13  KDEQEIAAASSSSNHGLHWIRGSVIGKGGFASVFMASLKPSVAERSIYPSVMAVKSAEVS 72
           K+  ++   S+  N+G+ W RG +IGKG F SVF+A LKP   + SI+P VMAVKSAE+S
Sbjct: 9   KEAAQVEEGSTEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFTKYSIFPPVMAVKSAEIS 68

Query: 73  DSGTLQKEKLAYDNLHSCESLIQCYGEETTFNDHGQMIYNLLLEFAEGGDLAHRIKGTSG 132
            S TLQKEK  YDNL  C SLIQC+GEE T + +G+MIYNLLLE A GG LAH IK T G
Sbjct: 69  VSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMIYNLLLEVATGGTLAHHIKNTGG 128

Query: 133 KGLPEIEVRKYTRSIIKGLIHIHKSKYVHCDLKPANILLVPR-NSSKGQRFTAKIGDLGS 192
           KGL E  VR YT+SIIKGLIHIH+S+YVHCDLKPANILL+P+ N++K ++F AKI DLG 
Sbjct: 129 KGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQFIAKIADLGL 188

Query: 193 ARRINKTKPFYSLGGTVMYMAPETLIHNVQESPSDIWALGCLVLEMLTGNRPWTAADRAG 252
           ARR +KTK  Y LGGT  YMAPETLI  VQES SDIWALGC+VLEMLTGNR W A D+ G
Sbjct: 189 ARRTSKTKASYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGNRAWAATDKVG 248

Query: 253 IAKQIVEKH-RMPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFVVAATGEEDD- 312
           I +++ E    MPKIP  +S EA  FLK C VRKP+ R TAEML+N PFV AA  +E D 
Sbjct: 249 ILQEMTENFLGMPKIPEGLSAEATGFLKNCLVRKPEFRFTAEMLINVPFVAAAEDQEQDF 308

Query: 313 DFVAAPTSVEINRRSSKRLRV 331
           + V APT V    R  KR R+
Sbjct: 309 NTVKAPTFVTKWPRQFKRQRI 329

BLAST of Sed0027297 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 2.1e-50
Identity = 109/279 (39.07%), Postives = 163/279 (58.42%), Query Frame = 0

Query: 29  LHWIRGSVIGKGGFASVFMASLKPSVAERSIYPSVMAVKSAEVSDSGTLQKEKLAYDNLH 88
           + W+RG  IG G F++V  A+   +  +   +P+++AVKS +   + +L  EK   D+L 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGD---FPALIAVKSTDAYGAASLSNEKSVLDSLG 60

Query: 89  SCESLIQCYGEETTFNDHGQMIYNLLLEFAEGGDLAHRIKGTSGKGLPEIEVRKYTRSII 148
            C  +I+CYGE++T  ++G+ ++NLLLE+A  G LA  +K   G+GLPE  VR++T S++
Sbjct: 61  DCPEIIRCYGEDSTV-ENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVL 120

Query: 149 KGLIHIHKSKYVHCDLKPANILLVPRNSSKGQRFTAKIGDLGSARRIN----KTKPFYSL 208
           +GL HIH   + HCD+K ANILL    S        KI D G A R++      +    +
Sbjct: 121 RGLRHIHAKGFAHCDIKLANILLFNDGS-------VKIADFGLAMRVDGDLTALRKSVEI 180

Query: 209 GGTVMYMAPETLIHNVQESPSDIWALGCLVLEMLTGNRPWTAADRAGIAKQIVE---KHR 268
            GT +YMAPE +  N   S +D+WALGC V+EM +G   W+  + +     ++       
Sbjct: 181 RGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDE 240

Query: 269 MPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFV 301
           +PKIP  +SEE + FL  CFV+ P  R TAEML+N  FV
Sbjct: 241 LPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFV 268

BLAST of Sed0027297 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 2.9e-39
Identity = 104/277 (37.55%), Postives = 154/277 (55.60%), Query Frame = 0

Query: 29  LHWIRGSVIGKGGFASVFMASLKPSVAERSIYPSVMAVKSAEVSDSGTLQKEKLAYDNLH 88
           + W RG ++G+G  A+V+ A+   S         ++AVKS+EV  S  LQ+E     +L 
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNS-------DEILAVKSSEVHRSEFLQREAKILSSL- 60

Query: 89  SCESLIQCYGEETTFNDHGQMIYNLLLEFAEGGDLAHRIKGTSGKGLPEIEVRKYTRSII 148
           S   +I   G ET    +G ++YNLL+E+A  G L        G+ + E  V KYTR I+
Sbjct: 61  SSPYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGR-VDETRVVKYTRDIL 120

Query: 149 KGLIHIHKSKYVHCDLKPANILLVPRNSSKGQRFTAKIGDLGSARRINKTKPFYS-LGGT 208
           KGL +IH    VHCD+K +N+++    S KG+   AKI D G A+R++    F S + GT
Sbjct: 121 KGLEYIHSKGIVHCDVKGSNVVI----SEKGE---AKIADFGCAKRVDPV--FESPVMGT 180

Query: 209 VMYMAPETLIHNVQESPSDIWALGCLVLEMLTGNRPWTAADR----AGIAKQIVEKHRMP 268
             +MAPE      Q   SDIWA+GC ++EM+TG+ PWT AD       +  ++      P
Sbjct: 181 PAFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETP 240

Query: 269 KIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFV 301
           ++P  ++EEA+ FL+ C  R+   R TA  L+N PF+
Sbjct: 241 ELPCLLAEEAKDFLEKCLKREANERWTATQLLNHPFL 259

BLAST of Sed0027297 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.8e-34
Identity = 107/301 (35.55%), Postives = 161/301 (53.49%), Query Frame = 0

Query: 29  LHWIRGSVIGKGGFASVFMASLKPSVAERSIYPSVMAVKSAEVSDSGTLQKEKLAYDNLH 88
           ++W RG  +G+G  A+V  A+   S          +AVKSAE   S  LQ+E     +L+
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHES-------GETLAVKSAEFHRSEFLQREAKILSSLN 60

Query: 89  SCESLIQCYGEETT---FNDHGQ-MIYNLLLEFAEGGDLAHRIKGTSGKGLPEIEVRKYT 148
           S   +I   G E T   F+++G+   Y+LL+E+A  G L   +   +G  + E  V KYT
Sbjct: 61  S-PYVIGYRGCEITREPFHNNGEATTYSLLMEYAPYGTLTD-VATKNGGFIDEARVVKYT 120

Query: 149 RSIIKGLIHIHKSKYV-HCDLKPANILLVPRNSSKGQRFTAKIGDLGSARRINK--TKPF 208
           R I+ GL +IH SK + HCD+K +N+L+       G+   AKI D G A+ +    T+P 
Sbjct: 121 RQILLGLEYIHNSKGIAHCDIKGSNVLV-------GENGEAKIADFGCAKWVEPEITEP- 180

Query: 209 YSLGGTVMYMAPETLIHNVQESPSDIWALGCLVLEMLTGNRPWTAA---DRAGIAKQIVE 268
             + GT  +MAPE      Q   SDIWA+GC V+EM+TG++PW  A   D   +  ++  
Sbjct: 181 --VRGTPAFMAPEAARGERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGY 240

Query: 269 KHRMPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFVVAATGEEDDDFVA-APTS 319
              +P++P  ++E+A+ FL  C  ++   R TA  L+N PF+V    E     V  +PTS
Sbjct: 241 LGELPELPCSLTEQAKDFLGKCLKKEATERWTASQLLNHPFLVNKEPELVTGLVTNSPTS 282

BLAST of Sed0027297 vs. ExPASy Swiss-Prot
Match: Q9C5H5 (Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK5 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.1e-30
Identity = 100/319 (31.35%), Postives = 156/319 (48.90%), Query Frame = 0

Query: 31  WIRGSVIGKGGFASVFMASLKPSVA-----ERSIYPSVMAVKSAEVSDSGTLQKEKLAYD 90
           W +G +IG+G F SV++AS   + A     E  ++P     KSAE      L++E     
Sbjct: 346 WKKGKLIGRGTFGSVYVASNSETGALCAMKEVELFPD--DPKSAECIKQ--LEQEIKLLS 405

Query: 91  NLHSCESLIQCYGEETTFNDHGQMIYNLLLEFAEGGDLAHRIKGTSGKGLPEIEVRKYTR 150
           NL    +++Q +G ET      +  + + LE+   G +   I+   G  + E  VR +TR
Sbjct: 406 NLQH-PNIVQYFGSETV-----EDRFFIYLEYVHPGSINKYIRDHCGT-MTESVVRNFTR 465

Query: 151 SIIKGLIHIHKSKYVHCDLKPANILLVPRNSSKGQRFTAKIGDLGSARRINKTKPFYSLG 210
            I+ GL ++H  K VH D+K AN+L+     + G     K+ D G A+ +   +   SL 
Sbjct: 466 HILSGLAYLHNKKTVHRDIKGANLLV----DASG---VVKLADFGMAKHLTGQRADLSLK 525

Query: 211 GTVMYMAPETLIHNVQESPS-------DIWALGCLVLEMLTGNRPWTAADRAGIAKQIVE 270
           G+  +MAPE +   +Q+  +       DIW+LGC ++EM TG  PW+  +  G A     
Sbjct: 526 GSPYWMAPELMQAVMQKDSNPDLAFAVDIWSLGCTIIEMFTGKPPWSEFE--GAAAMFKV 585

Query: 271 KHRMPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFV---VAATGEEDDDFVAAP 330
               P IP  +S E + FL+ CF R P  RPTA ML+   F+   +  T   + D     
Sbjct: 586 MRDSPPIPESMSPEGKDFLRLCFQRNPAERPTASMLLEHRFLKNSLQPTSPSNSDVSQLF 644

Query: 331 TSVEINRRSSKRLRVSFKI 335
             + I   SS+R + +FK+
Sbjct: 646 NGMNITEPSSRREKPNFKL 644

BLAST of Sed0027297 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 135.6 bits (340), Expect = 1.1e-30
Identity = 93/326 (28.53%), Postives = 152/326 (46.63%), Query Frame = 0

Query: 25  SNHGLHWIRGSVIGKGGFASVFMASLKPSVAERSIYPSVMAVKSAEVSDSGTLQKEKLAY 84
           SNH + W +G ++G+GG+ SV++   K +         + AVK  E+ D  +  K K   
Sbjct: 164 SNHNIKWQKGQILGRGGYGSVYLGLNKDT-------GELFAVKQLEIVDINSDPKLKNMI 223

Query: 85  DNLHSCESLIQCYGEETTFNDHG----QMIYNLLLEFAEGGDLAHRIKGTSGKGLPEIEV 144
            +      +++    +      G    Q   ++ LE+  GG ++  + G  G    E  +
Sbjct: 224 LSFSKEIEVMRSLRHDNIVRYLGTSLDQSFLSVFLEYIPGGSIS-SLLGKFG-AFSENVI 283

Query: 145 RKYTRSIIKGLIHIHKSKYVHCDLKPANILLVPRNSSKGQRFTAKIGDLGSARRINK-TK 204
           + YT+ I++GL  +H +  +H D+K ANIL+     +KG     K+ D G ++  +    
Sbjct: 284 KVYTKQILQGLSFLHANSIIHRDIKGANILI----DTKG---IVKLSDFGCSKSFSGIVS 343

Query: 205 PFYSLGGTVMYMAPETLIHNVQESPSDIWALGCLVLEMLTGNRPWT-AADRAGIAKQIVE 264
            F S+ GT  +MAPE +        SDIW+LGC+++EM T   PW+   + A +   I  
Sbjct: 344 QFKSMQGTPYWMAPEVIKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIAS 403

Query: 265 KHRMPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFVVAATGEEDDDFVAAPTSV 324
            + +P IP  +S+EA  FL  CF R PK RP A  L+  PF++       DD +  PT  
Sbjct: 404 SNSIPNIPSHMSQEAFDFLNLCFKRDPKERPDANQLLKHPFIMNL-----DDNIQLPTIS 463

Query: 325 EINRRSSKRLRVSFKIVRKSNIATKN 345
                S+     +      +N    N
Sbjct: 464 PTTTLSTNTTNTTATTTTTNNATNSN 468

BLAST of Sed0027297 vs. ExPASy TrEMBL
Match: A0A5D3CF40 (Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001430 PE=3 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 2.5e-102
Identity = 211/334 (63.17%), Postives = 244/334 (73.05%), Query Frame = 0

Query: 1   MKRSSNSMEDDSKDEQEIAAASSSSNHGLHWIRGSVIGKGGFASVFMASLKPSVA-ERSI 60
           MKR  NSM ++   E E+   SS  N+G+ W RG +IGKG F SVF+ASLKP ++ + SI
Sbjct: 1   MKR--NSMLEEL--EVEVEDGSSEFNNGIQWKRGRLIGKGSFGSVFLASLKPHISIKYSI 60

Query: 61  YPSVMAVKSAEVSDSGTLQKEKLAYDNLHSCESLIQCYGEETTFNDHGQMIYNLLLEFAE 120
           +PSVMAVKSAE+S S TLQKEK  YDNL  C SLIQC+GEE T + +G MIYNLLLE A 
Sbjct: 61  FPSVMAVKSAEISVSETLQKEKQNYDNLKGCSSLIQCFGEEITTDHNGHMIYNLLLEVAT 120

Query: 121 GGDLAHRIKGTSGKGLPEIEVRKYTRSIIKGLIHIHKSKYVHCDLKPANILLVPRNSSKG 180
           GG LAH IK T GKGL E  VR YT+SIIKGLIHIH+S+YVHCDLKPANILL+P+N++  
Sbjct: 121 GGTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLIPKNNTTT 180

Query: 181 QR-FTAKIGDLGSARRINKTKPFYSLGGTVMYMAPETLIHNVQESPSDIWALGCLVLEML 240
            R F AKI DLG ARR +KTK  Y LGGT  YMAPETLI  VQES SDIWALGC+VLEML
Sbjct: 181 NRQFIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCIVLEML 240

Query: 241 TGNRPWTAADRAGIAKQIVEK-HRMPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNF 300
           TGNR W A D+ GI K++ E  H MPKIP  +S EA  FLK C VRKP+ R TAEMLMN 
Sbjct: 241 TGNRAWAATDKVGIVKEMTENLHGMPKIPDGLSVEATGFLKNCLVRKPEFRFTAEMLMNV 300

Query: 301 PFVVAATGEEDD-DFVAAPTSVEINRRSSKRLRV 331
           PFV AA  +E D + + APTSV    R  KR R+
Sbjct: 301 PFVAAAVDQEQDFNTMKAPTSVTKWPRQFKRQRI 330

BLAST of Sed0027297 vs. ExPASy TrEMBL
Match: A0A1S3CED5 (mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103500026 PE=3 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 3.2e-102
Identity = 211/334 (63.17%), Postives = 244/334 (73.05%), Query Frame = 0

Query: 1   MKRSSNSMEDDSKDEQEIAAASSSSNHGLHWIRGSVIGKGGFASVFMASLKPSVA-ERSI 60
           MKR  NSM ++   E E+   SS  N+G+ W RG +IGKG F SVF+ASLKP ++ + SI
Sbjct: 1   MKR--NSMLEEL--EVEVEDGSSEFNNGIQWKRGRLIGKGSFGSVFLASLKPHISIKYSI 60

Query: 61  YPSVMAVKSAEVSDSGTLQKEKLAYDNLHSCESLIQCYGEETTFNDHGQMIYNLLLEFAE 120
           +PSVMAVKSAE+S S TLQKEK  YDNL  C SLIQC+GEE T + +G MIYNLLLE A 
Sbjct: 61  FPSVMAVKSAEISVSETLQKEKQNYDNLKGCSSLIQCFGEEITTDHNGHMIYNLLLEVAT 120

Query: 121 GGDLAHRIKGTSGKGLPEIEVRKYTRSIIKGLIHIHKSKYVHCDLKPANILLVPRNSSKG 180
           GG LAH IK T GKGL E  VR YT+SIIKGLIHIH+S+YVHCDLKPANILL+P+N++  
Sbjct: 121 GGTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLIPKNNTTT 180

Query: 181 QR-FTAKIGDLGSARRINKTKPFYSLGGTVMYMAPETLIHNVQESPSDIWALGCLVLEML 240
            R F AKI DLG ARR +KTK  Y LGGT  YMAPETLI  VQES SDIWALGC+VLEML
Sbjct: 181 NRQFIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEML 240

Query: 241 TGNRPWTAADRAGIAKQIVEK-HRMPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNF 300
           TGNR W A D+ GI K++ E  H MPKIP  +S EA  FLK C VRKP+ R TAEMLMN 
Sbjct: 241 TGNRAWAATDKVGIVKEMTENLHGMPKIPDGLSVEATGFLKNCLVRKPEFRFTAEMLMNV 300

Query: 301 PFVVAATGEEDD-DFVAAPTSVEINRRSSKRLRV 331
           PFV AA  +E D + + APTSV    R  KR R+
Sbjct: 301 PFVAAAVDQEQDFNTMKAPTSVTKWPRQFKRQRI 330

BLAST of Sed0027297 vs. ExPASy TrEMBL
Match: A0A6J1DPQ9 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111021943 PE=3 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 2.3e-100
Identity = 204/334 (61.08%), Postives = 243/334 (72.75%), Query Frame = 0

Query: 8   MEDDSKDEQEIAAASSSSNHGLHWIRGSVIGKGGFASVFMASLKPSVAERSIYPSVMAVK 67
           M+  S +  EI    +  NHG+ W RG +IGKG F SVFMASL+  +++ SI+P VMAVK
Sbjct: 1   MKRKSTENDEI--VPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVK 60

Query: 68  SAEVSDSGTLQKEKLAYDNLHSCESLIQCYGEETTFNDHGQMIYNLLLEFAEGGDLAHRI 127
           SAEVSDS TLQKEK  YDNL  C+SLI+C+GEE T +++GQMIYNLLLEFA  G LA +I
Sbjct: 61  SAEVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQI 120

Query: 128 KGTSGKGLPEIEVRKYTRSIIKGLIHIHKSKYVHCDLKPANILLVPRNSSKGQRFTAKIG 187
           K T GKGL E EV  YTRSIIKGLIHIHK++YVHCDLKP NILLVPRNS+ G+R  AKIG
Sbjct: 121 KNTGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIG 180

Query: 188 DLGSARRINKTKPFYSLGGTVMYMAPETLIHNVQESPSDIWALGCLVLEMLTGNRPWTAA 247
           DLG A+R +KTK    L GTV YMAPETL+++VQESPSDIWALGC+VLEMLTGNRPW+  
Sbjct: 181 DLGLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGG 240

Query: 248 ----DRAGIAKQIVEKHRMPKIP--VEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFVV 307
               D   I ++  EK+ +P IP    +S EAR FLK CFVRKP+ R TAEMLM  PF+ 
Sbjct: 241 GGGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLA 300

Query: 308 AATGEEDDDFVAAPTSVEINRRSSKRLRVSFKIV 336
               EE++  V AP  VE  RR SKR R+  + V
Sbjct: 301 EDEEEEEESVVGAPRFVEKARR-SKRQRIPIQAV 331

BLAST of Sed0027297 vs. ExPASy TrEMBL
Match: A0A0A0KBM3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G430800 PE=3 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 6.7e-100
Identity = 200/320 (62.50%), Postives = 231/320 (72.19%), Query Frame = 0

Query: 13  KDEQEIAAASSSSNHGLHWIRGSVIGKGGFASVFMASLKPSVAERSIYPSVMAVKSAEVS 72
           K+  ++   SS  N+G+ W +G +IGKG F SVF+ASLKP   + SI+P VMAVKSAE+S
Sbjct: 9   KEAAQVEDRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPPVMAVKSAEIS 68

Query: 73  DSGTLQKEKLAYDNLHSCESLIQCYGEETTFNDHGQMIYNLLLEFAEGGDLAHRIKGTSG 132
            S TLQKEK  YDNL  C SLIQC+GEE T + +G MIYNLLLE A GG LAH IK T G
Sbjct: 69  VSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGTLAHHIKNTGG 128

Query: 133 KGLPEIEVRKYTRSIIKGLIHIHKSKYVHCDLKPANILLVPR-NSSKGQRFTAKIGDLGS 192
           KGL E  VR YT+SIIKGLIHIH+S+YVHCDLKPANILL+P+ N++K ++F AKI DLG 
Sbjct: 129 KGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQFIAKIADLGL 188

Query: 193 ARRINKTKPFYSLGGTVMYMAPETLIHNVQESPSDIWALGCLVLEMLTGNRPWTAADRAG 252
           ARR +KTK  Y LGGT  YMAPETLI  VQES SDIWALGC+VLEMLTGNR W A D+ G
Sbjct: 189 ARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGNRAWAATDKVG 248

Query: 253 IAKQIVEKH-RMPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFVVAATG-EEDD 312
           I K++ E    MPKIP  +S EA  FLK C VRKP+ R TAEMLMN PFV A    E+D 
Sbjct: 249 IVKEMTENFIGMPKIPEGLSPEATGFLKNCLVRKPEFRFTAEMLMNVPFVAAGEDHEQDS 308

Query: 313 DFVAAPTSVEINRRSSKRLR 330
             V APT V    R  KR R
Sbjct: 309 TTVKAPTFVTKWPRQFKRQR 328

BLAST of Sed0027297 vs. ExPASy TrEMBL
Match: A0A1S3CFZ6 (mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103500025 PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 8.8e-100
Identity = 198/321 (61.68%), Postives = 234/321 (72.90%), Query Frame = 0

Query: 13  KDEQEIAAASSSSNHGLHWIRGSVIGKGGFASVFMASLKPSVAERSIYPSVMAVKSAEVS 72
           K+  ++   S+  N+G+ W RG +IGKG F SVF+A LKP   + SI+P VMAVKSAE+S
Sbjct: 9   KEAAQVEEGSTEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFTKYSIFPPVMAVKSAEIS 68

Query: 73  DSGTLQKEKLAYDNLHSCESLIQCYGEETTFNDHGQMIYNLLLEFAEGGDLAHRIKGTSG 132
            S TLQKEK  YDNL  C SLIQC+GEE T + +G+MIYNLLLE A GG LAH IK T G
Sbjct: 69  VSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMIYNLLLEVATGGTLAHHIKNTGG 128

Query: 133 KGLPEIEVRKYTRSIIKGLIHIHKSKYVHCDLKPANILLVPR-NSSKGQRFTAKIGDLGS 192
           KGL E  VR YT+SIIKGLIHIH+S+YVHCDLKPANILL+P+ N++K ++F AKI DLG 
Sbjct: 129 KGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQFIAKIADLGL 188

Query: 193 ARRINKTKPFYSLGGTVMYMAPETLIHNVQESPSDIWALGCLVLEMLTGNRPWTAADRAG 252
           ARR +KTK  Y LGGT  YMAPETLI  VQES SDIWALGC+VLEMLTGNR W A D+ G
Sbjct: 189 ARRTSKTKASYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGNRAWAATDKVG 248

Query: 253 IAKQIVEKH-RMPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFVVAATGEEDD- 312
           I +++ E    MPKIP  +S EA  FLK C VRKP+ R TAEML+N PFV AA  +E D 
Sbjct: 249 ILQEMTENFLGMPKIPEGLSAEATGFLKNCLVRKPEFRFTAEMLINVPFVAAAEDQEQDF 308

Query: 313 DFVAAPTSVEINRRSSKRLRV 331
           + V APT V    R  KR R+
Sbjct: 309 NTVKAPTFVTKWPRQFKRQRI 329

BLAST of Sed0027297 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 218.4 bits (555), Expect = 9.1e-57
Identity = 123/294 (41.84%), Postives = 177/294 (60.20%), Query Frame = 0

Query: 29  LHWIRGSVIGKGGFASVFMASLKPSVAERSIYPSVMAVKSAEVSDSGTLQKEKLAYDNLH 88
           + WIR   IG G F++V +A+   S ++   +PS+MAVKS+ V  S  L+ E+   D+L 
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSK--AFPSLMAVKSSGVVCSAALRNERDVLDDLG 60

Query: 89  SCESLIQCYGEETTFNDHGQMIYNLLLEFAEGGDLAHRIKGTSGKGLPEIEVRKYTRSII 148
            C  +++C+GE  T  ++G+ IYNL LE+A GG LA RIK +SG+ LPE EVR++TRSI+
Sbjct: 61  DCSEIVRCFGEGRTV-ENGEEIYNLFLEYASGGSLADRIK-SSGEALPEFEVRRFTRSIV 120

Query: 149 KGLIHIHKSKYVHCDLKPANILLVPRNSSKGQRFTAKIGDLGSARRINKTKPFYSLGGTV 208
           KGL HIH + + HCD+K  N+L+             KI D G A+R    +    + GT 
Sbjct: 121 KGLCHIHGNGFTHCDIKLENVLVFGDGD-------VKISDFGLAKR-RSGEVCVEIRGTP 180

Query: 209 MYMAPETLIHNVQESPSDIWALGCLVLEMLTGNRPWTAADRA-----GIAKQIVEKHRMP 268
           +YMAPE++ H   ESP+DIWALGC V+EM +G   W   D        +  +I     +P
Sbjct: 181 LYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVP 240

Query: 269 KIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFVVA--ATGEEDDDFVAAP 316
           +IPVE+SEE + F+  CFV+    R TAEML++ PF+     +GEED+    +P
Sbjct: 241 RIPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDESGEEDEACSVSP 282

BLAST of Sed0027297 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 202.2 bits (513), Expect = 6.7e-52
Identity = 114/295 (38.64%), Postives = 170/295 (57.63%), Query Frame = 0

Query: 29  LHWIRGSVIGKGGFASVFMASLKPSVAERSIYPSVMAVKSAEVSDSGTLQKEKLAYDNL- 88
           + WIRG  IG G F++V +A+   S  +   +P +MAVKSA+   + +L  EK   DNL 
Sbjct: 1   MEWIRGETIGYGTFSTVSLAT--RSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLG 60

Query: 89  HSCESLIQCYGEETTFNDHGQMIYNLLLEFAEGGDLAHRIKGTSGKGLPEIEVRKYTRSI 148
             C  +++C+GE+ T  ++G+ ++NL LE+A  G L   +K  +G+G+PE  VR++T S+
Sbjct: 61  DDCNEIVRCFGEDRTV-ENGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSV 120

Query: 149 IKGLIHIHKSKYVHCDLKPANILLVPRNSSKGQRFTAKIGDLGSARRINKTKPF---YSL 208
           ++GL HIH + + HCDLK  NILL    +        KI D G A+RI           +
Sbjct: 121 LRGLRHIHANGFAHCDLKLGNILLFGDGA-------VKIADFGLAKRIGDLTALNYGVQI 180

Query: 209 GGTVMYMAPETLIHNVQESPSDIWALGCLVLEMLTGNRPWTAADRAGIAKQIVE---KHR 268
            GT +YMAPE++  N   S  D+WALGC+V+EM +G   W+  + +     ++       
Sbjct: 181 RGTPLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDE 240

Query: 269 MPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFVVAATGEE----DDDFV 313
           +P IP E+SE+ R FL  CFV+ PK R TAEML+N PFV      +    ++DFV
Sbjct: 241 VPMIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFV 285

BLAST of Sed0027297 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 201.1 bits (510), Expect = 1.5e-51
Identity = 109/279 (39.07%), Postives = 163/279 (58.42%), Query Frame = 0

Query: 29  LHWIRGSVIGKGGFASVFMASLKPSVAERSIYPSVMAVKSAEVSDSGTLQKEKLAYDNLH 88
           + W+RG  IG G F++V  A+   +  +   +P+++AVKS +   + +L  EK   D+L 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGD---FPALIAVKSTDAYGAASLSNEKSVLDSLG 60

Query: 89  SCESLIQCYGEETTFNDHGQMIYNLLLEFAEGGDLAHRIKGTSGKGLPEIEVRKYTRSII 148
            C  +I+CYGE++T  ++G+ ++NLLLE+A  G LA  +K   G+GLPE  VR++T S++
Sbjct: 61  DCPEIIRCYGEDSTV-ENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVL 120

Query: 149 KGLIHIHKSKYVHCDLKPANILLVPRNSSKGQRFTAKIGDLGSARRIN----KTKPFYSL 208
           +GL HIH   + HCD+K ANILL    S        KI D G A R++      +    +
Sbjct: 121 RGLRHIHAKGFAHCDIKLANILLFNDGS-------VKIADFGLAMRVDGDLTALRKSVEI 180

Query: 209 GGTVMYMAPETLIHNVQESPSDIWALGCLVLEMLTGNRPWTAADRAGIAKQIVE---KHR 268
            GT +YMAPE +  N   S +D+WALGC V+EM +G   W+  + +     ++       
Sbjct: 181 RGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDE 240

Query: 269 MPKIPVEVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFV 301
           +PKIP  +SEE + FL  CFV+ P  R TAEML+N  FV
Sbjct: 241 LPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFV 268

BLAST of Sed0027297 vs. TAIR 10
Match: AT2G42550.1 (Protein kinase superfamily protein )

HSP 1 Score: 174.9 bits (442), Expect = 1.2e-43
Identity = 105/312 (33.65%), Postives = 165/312 (52.88%), Query Frame = 0

Query: 39  KGGFASVFMASLKPSVAERSIYPSVMAVKSAEVSDSGTLQKEKLAYDNLHSCESLIQCYG 98
           KG + SV +  +K    + +  P   AVK+AE  D  +L++E      L  C  ++QCYG
Sbjct: 13  KGAYGSVDL--VKYIKRDDNALPLYAAVKTAECEDYNSLEREIQILSKLEGCRRIVQCYG 72

Query: 99  E---ETTFNDHGQMIYNLLLEFAEGGDLAHRIKGTSGKGLPEIEVRKYTRSIIKGLIHIH 158
               E  F+  G  +Y +++E+A  G L   +     + LPE  ++ +TR I++GL+ +H
Sbjct: 73  NYTLEEDFDVGGFRVYKMVMEYAAAGSLFSFMDSYKDRKLPETMIKDFTRMILQGLVSVH 132

Query: 159 KSKYVHCDLKPANILLVPRNSSKGQRFTAKIGDLGSARRINKTKPFYSLG----GTVMYM 218
           +  YVHCDLKP N+L+ P      Q +  KI D GS+R++ +    + +     GT +YM
Sbjct: 133 RLGYVHCDLKPDNLLVFPCR----QSYELKISDFGSSRKVGEYSDCWDVDLPFVGTPVYM 192

Query: 219 APETLIHNVQESPSDIWALGCLVLEMLTGNRPWTAADRAGIAKQIVEKHRMPKIPVEVSE 278
           +PE++   V E   D+W+LGC+VLEM TG  PW+  +   +A  +  K + P+IP  +  
Sbjct: 193 SPESVRSGVAEKALDLWSLGCIVLEMYTGVIPWSEVEFEDLAPAL-SKGKAPEIPKSLPC 252

Query: 279 EARWFLKCCFVRKPKLRPTAEMLMNFPFVVAATGEEDDDFVAAPTSVEINRRSSKRLRVS 338
           +AR FL+ CF R PK R +A  L++  F+    GE    F   P  ++I     K   VS
Sbjct: 253 DARKFLETCFSRNPKERGSASDLLSHQFL---RGEVVSGFSLPPLKLKIKLAPEKPTNVS 312

Query: 339 FKIVRKSNIATK 344
            K ++   I  K
Sbjct: 313 KKPLKLKIIPPK 314

BLAST of Sed0027297 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 171.4 bits (433), Expect = 1.3e-42
Identity = 107/298 (35.91%), Postives = 160/298 (53.69%), Query Frame = 0

Query: 29  LHWIRGSVIGKGGFASVFMASLKPSVAERSIYPSVMAVKSAEVSDSGTLQKEKLAYDNLH 88
           ++W RG +IG+G  A+V +A         S    + AVKSA++S S  LQKE+     L 
Sbjct: 3   INWTRGPIIGRGSTATVSIAI--------SSSGELFAVKSADLSSSSLLQKEQSILSTL- 62

Query: 89  SCESLIQCYGEETTFNDHGQMIYNLLLEFAEGGDLAHRIKGTSGKGLPEIEVRKYTRSII 148
           S   +++  G   T   +G ++YN+L+E+  GG+L   IK + GK LPE E+R YTR I+
Sbjct: 63  SSPHMVKYIGTGLTRESNG-LVYNILMEYVSGGNLHDLIKNSGGK-LPEPEIRSYTRQIL 122

Query: 149 KGLIHIHKSKYVHCDLKPANILLVPRNSSKGQRFTAKIGDLGSARRINKTKPFYSLGGTV 208
            GL+++H+   VHCDLK  N+L+        +    KI D+G A+ ++K++      GT 
Sbjct: 123 NGLVYLHERGIVHCDLKSHNVLV-------EENGVLKIADMGCAKSVDKSE----FSGTP 182

Query: 209 MYMAPETLIHNVQESPSDIWALGCLVLEMLTGNRPWTAA-DRAGIAKQIVEKHRMPKIPV 268
            +MAPE      Q  P+D+WALGC ++EM+TG+ PW    D      +I      P IP 
Sbjct: 183 AFMAPEVARGEEQRFPADVWALGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPA 242

Query: 269 EVSEEARWFLKCCFVRKPKLRPTAEMLMNFPFVVAATGEEDDDFVAAPTSVEINRRSS 326
            +S++A+ FLK C     K R T E L+  PF+       DDD  +  +    N+ SS
Sbjct: 243 WISDKAKDFLKNCLKEDQKQRWTVEELLKHPFL-------DDDEESQTSDCLKNKTSS 271

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0058687.15.1e-10263.17mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa]... [more]
XP_008461431.16.7e-10263.17PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo][more]
KAG7014197.11.1e-10160.71Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper... [more]
XP_022154776.14.8e-10061.08mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia][more]
XP_008461430.11.8e-9961.68PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9SND62.1e-5039.07Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
O808882.9e-3937.55Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q9ZVP52.8e-3435.55Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
Q9C5H51.1e-3031.35Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702... [more]
Q54R821.1e-3028.53Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Match NameE-valueIdentityDescription
A0A5D3CF402.5e-10263.17Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuw... [more]
A0A1S3CED53.2e-10263.17mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN... [more]
A0A6J1DPQ92.3e-10061.08mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX... [more]
A0A0A0KBM36.7e-10062.50Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G430... [more]
A0A1S3CFZ68.8e-10061.68mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT4G36950.19.1e-5741.84mitogen-activated protein kinase kinase kinase 21 [more]
AT5G67080.16.7e-5238.64mitogen-activated protein kinase kinase kinase 19 [more]
AT3G50310.11.5e-5139.07mitogen-activated protein kinase kinase kinase 20 [more]
AT2G42550.11.2e-4333.65Protein kinase superfamily protein [more]
AT4G26890.11.3e-4235.91mitogen-activated protein kinase kinase kinase 16 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 31..300
e-value: 5.2E-54
score: 195.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 34..299
e-value: 7.1E-47
score: 160.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 31..300
score: 36.513081
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 29..346
e-value: 2.4E-54
score: 186.7
NoneNo IPR availablePIRSRPIRSR037014-2PIRSR037014-2coord: 108..288
e-value: 2.8E-8
score: 30.3
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 108..288
e-value: 2.8E-8
score: 30.3
NoneNo IPR availablePIRSRPIRSR000550-2PIRSR000550-2coord: 31..288
e-value: 1.3E-23
score: 81.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..17
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR48011:SF56MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3-LIKEcoord: 28..312
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 28..312
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 159..171
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 37..67
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 31..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0027297.1Sed0027297.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity