Sed0027136 (gene) Chayote v1

Overview
NameSed0027136
Typegene
OrganismSechium edule (Chayote v1)
DescriptiontRNA (Guanine(10)-N2)-methyltransferase homolog
LocationLG06: 3295534 .. 3300782 (-)
RNA-Seq ExpressionSed0027136
SyntenySed0027136
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGTATCTGTGTGTGTTCTATCACAGGCTATTGGATTACAGAAAGGCCGAGGTCGAATCTCTGGCCGAGTTGTTCGCAGAAAATGGCGACCAGTTGCAATGGAAGCTTCCCAAGCACCACCATCCCGATTCTCCATTCCATTTCGTCGACCTCTCTTCCGACGACCTAGCTCGGAAAATCGCAAATCGAAGTGAGCCATTTCGTTCCCTTTTTGTTAGGGTTTCTCTTAGTTTAGATTTTGTTTCTGAAAACTTGGGCTTTGAAGGTGTGATCCATTCGAAGTCCTAGGTTCGAGGCTCAACTTTGACACTAATTTGTAGACAGCTCGTGGCCTAGATAAGAATTTGAAACTTGGGTTTTGAAGGTGTACTCTCCTCGAAATCCTAGGTTCGAGGCTCAACTTTGACATTAATTTGTAGACACCTCCCGTCCCTCGTCTCCCACTGGCTGGCCTAGGGACGTGCATTACCTGTGTATAGTGAGTGAAGCTCTGATGAAAATGAAAAAATCCCCAAGGGATGGCACCTTGATCAAAAGCTTAGCTTTTGAAAGTGTGCACTCTTCAAGATTCCATGTTTGAAATTAGTACTAACATTAATTTATGCTCTCCTCGAGGTCCCATATTCGAAATTAGTGGTGACATTAATTTGTAGACACCTTTAATGTCTCCATTCTTGAGCAATTGGGTAGCTAGAAGATTTTCTTAGTGGAGGGAGGCCCCGATTTATCGGTTTCTAAAAATAAAAAATAATAATTTGGATTTTGTTTCTTTCATTTGATTTGGTTTGGTTTTGTGTAGGTATACTTGTGAAGGGGATGTATGAACTATGGGGTGAAGGTGGTGACTACTTGGAGTTGGAGGAGTCTATTAGAAACTACCCTGATGAGCGGAAGTTGCCGTATTTACAACCCGGAAGCTCTTTCAAGATTTTTGTTGAGAGCTTTGGTAAGGCTATCAGTTTTGAGGAACAGACTGAACGTATTCGGGGGCTTGCTTACATTCCTTTCAAGGTCTAGTAAGCTTTCGGTTTTATAGTGTTTCTGCTGTTTTTGTTCTATAGTTATAAATGTGTTTCTACATTTTGTGCTGCAATAATGTTGAGTTTGAGGAGTTAAAGTGTAAGTGTGACGAATTCGAGGGAGTTAGCTAGAAGTTACCAATTATGGTGTACCATTAATGTTTTAGGTAGTAAATTGATAAACGAAAGAATTGCAGTCTTGCAGAGCACAGAAGTAATTTGTGATTTGGAACTATCGAAAAGCTATAAGCACTAAGATTTGATTGAAGACAATATGGCAATATCTGTTTTGTAAATCTTATTAATCATTGTAGAAGCTCTTTGTGATCTTTGATAGGCCATTTGTACTATTTTCATTCATCAATAGAAGTAATTTTTCCTATAAAAAAAAGACATTATGGCAATATCGTATTCGTTCAGTGGTGTGCTAAGAAAGGGGGAGAGGGTGAGCAAGTGTGGATGGGCCCGTTATATCTCATATTTGGGAAATAATTTTTTTAACAATAATTTTAAAACATTGAGAAACAAAAGCTTGGATACTTTCTAGCATAATATTTGAATGGTTGTGGAAGTGTTGATCAATAATAGTTTGGTTTTGGTGGATATATCCAAAAGAAATAACAGCTGAAGATTAGATCTTGAAACCTCTCCAAAAGCACCACTCATGTTGAATTAAGAAAACATAGAGTTTCAACAAGGACTTGGATGAAGCACCCTGAGTTGGCTCAGTAGTAAAAAAATGGATAGGTAAACAATAAAGGGTTTATAAGGAATGAGTTAAAGTCATGTTGACTACCAACCTAGGATTTAATATCTTATGAGTTACCTTGACAAATGAATGTAGTTGGGTTAGACGTTTGTCTCATGAGGTTACTTGAATGCGTGCAAGCTGACTTCGATCACTCACTAATATAAAAAGCATGGGAGAGAGAGACGCATCAACCAACAAATATCCTTCTCCACTCATTACTTACAATACTAACCAACCAACTCTCTCTTATTTTCCCAATCCCCACAAAACTGGATTTCTCTTCATCCTTTTTTTTGTGTCCGTCCGTTCAGCCATTTTGCCCGCAACTCGACTAATGTCACAACCCCCGAGAAATACCGCCTGGCCCTACACATTTGGATGTTAAGGAAGCTCGTAGGAAATTAATTCTTTGGTAGGCAGCCATCATGAATTTAACCCATCATCTCTCTTCATCCATTTGTATTCTTTCAAAAAAAAAAGGAAAAATATATATTTTTCCGAATGTAATTATAATAAAAAAATATCGAAGGTATATAACTTAGTTCCTGCATGCAAGTATAATTGAGAAGGTATTGTCCGTCTACCTAACTTCGTACTTGTTTCATGCATGCAAATTTGATTTAGAAAGTATAATCAGTTTGCTTAATTATGTAAATTTATTTTAATCATGTTTCACATTTTAACCTGGAAATCTTACTCTTTTTTAAGAAACTCAGAGGCATGTTGATCCATTGGGGCAGCTATGCTAGCTACCTCATGCTTCATTTATGTTACAATTGCATCTGCTTTTACATTTACTTCTTAAATTAAGAGTTAAGATATGGTCTCATGCAATTATCATGGCTTCTCCTTGTAAATTGTATCAATCAATCAAAGTCGTAGAAGTCCTATGATTGTCATTTTCCTCTGTTAGCTCTTCTAGTTTCTGCAGGTAATACCTGAATTTTGACACTGTTTTTAATCATTCAGTTATCATTCGAACTATAGTGCATGTCAATGTCTTCAATTTCTCATAAATGAATATGGGTGGAATATTTCAGGGTCAGGTTAATTTAAAAAACCCAGATCACAAGTTCTACCTGATGGAAATGGCTGATTATGGATTGAATAATGGACTTCCGCCAATTTCTCAAAGGAGTATTTTTTTTGGACGGGAGGTTGGTGGTGCTGATAGGAAGCTTATACCGACATATCAGTTAAAAAGTCGCACTTATCTTGGGCCAACTGCCATGGATGCTGAAATGGCTTTCTTAATGGCCAACCAAGCATTAGCAACAGCAGGGAAACTTGTCTATGACCCATTTGTTGGCACTGGGAGCGTTCTAGTTGCTGCAGCTCATTTTGGAGCAATGACAATGGTAATTACTTTTGCTTTGTGTATATATATATATTTTTTTAAATATCGATATTTATGATAGATTGGGCCAGCTTACATTCATCTCAATTTTGTGTAAATTGCATTTAGAGAACATAGCTCATGCTGGTTTTCAATTTCCATAGCATGCGTGTGTGTGAAAGAGAAATCATTTCGCTTCAAACTGACCAAAATTTCTCAGGGTGCAGACATTGACATTAGGGTGGTACGTGATGGTCGTGGCCCTGATTGTAATGTTTGGAGCAACTTCAAGCAGGTAAGATTCTATGTTTAGAATTTTTTTTCGTGGTGCAAGTCTCGGTTTATAATAAACTGATCAAGTAATTTACTATGCTAAAAGTTTGAAACTTTATGCATGCATGAAGTAACAGTTAAAATACACACGAGGTGACAAACATGATAAAGAAAAGAAAATTTCTGTTAGCAATTCCTTGTGCAATTTCTTGAGAGTCAACTGATCACTATGGTATCAAGTTTTGATGTTTCATATTCCTATGCATGTAATTATGCTTTTATGATTTTTTTTGGGGGGTAAGAATTATGCTCTGTTTATGATGCTTCATGCAGTAATGGTAATTGGTACTCATCTGGTTATTACTTTTTGCAGTATGGATTGCCACCGCCAATTGCACTGTTAAGAGCAGATAATAATCTTCCTCCCTGGCGTCCAGGATTGAAAGAGGTTCAACATTAATACGCTCGCGCGCATATATATATATATATATATATATTAACCAAGCTTTCATTAAAAGATGCCTCCATTTCTTCCCCTTTCTAGGAAGAATTCTCGATCTCTGATTTTTATTTTTTATATCAATAACTTTGCTACATTTGGTTGTAAAAGAAAATCCATAGGAAATTAATTTAAGATGGCCATCATGAAATTAACCTATATCCTCTTAATTACCCAAAGTCCTCTTGACCACTAGGTTAGTCCATGATGGTTCGCTTATATGATTTTTCATCTTAATATTTTCAAATAATTTATTGAAACCTTATGTGAACCTTCATTTTACTGTTAGCCTTGTACAGTGGTTCATAAGGAGGCTGTGATGGCCTTATGTGGGATCCTAGATGCCCTATGAAAGTAAAATTTATTGTTTGGATTCTCATTTTCCGAGGCAGTTGATACATTGGGGAGAGATTAAAGTTGTGTTCCTAAGCATGGAACTTAGTCGGGATTTAATTATTTATAATTATATTATTGATAAATGTTTGGGCTAGTTTACGTACATTTGACTAAACTAACAAAACAACCGCACACCTTGACTAATCTTATTAAAGAATTGTTTGACTTTACTACATTCAGTTGACTGGAAACATGTAGGATATTAAATTATAGTAGGTGGGTTGTTTGAACTTTGTAAGATCTCACCAGGGTCAGAAAAGGAGATTGCAAAAGCAGCATATCCTTCCAAAGTAGACTATTTTAAACACTTGTTGTTGAGATGCTTATTATAAGTTTTTTGAGATAAACGTTAAAACACTATATTGGTGCTGATTCTGATTCTGATACCTTTTTATAACTTGTTTCTCTACCCAGATATTTGATGCAATCATTTGTGACCCTCCTTATGGAGTGCGTGCTGGGGGGCGTAAATCTGGTGGTCGGAAATTGTTAAAAGGAACTGTTGCTCCATACACCGTTCCCGACGACAAGAGAACAGACCATATACCATCAACTGCAGCTTACAGCCTTGTTGAGTGTGTGCATGATTTACTTGACCTCGCCGCCAAGATGCTCGTAATAGGTGGCAGGCTTGTATTCTTCTACCCTGTGTTGAGGGAAGAAGATAGTGTGTTCAACCAATTTCCGGAGCATCCATGTTTTACTCTAATCAGTTCTTCCGAGCAGATATTGAGCTCTCGATACAGCCGGGTGTTGCTAACTATGGTCAAAAGAACTTCGTACACCGAAGAAATGGCGGAAGCAGCAAGATTAAAACACCAAGAATTCAGGGAGAACCATTTGAAATGGTTAGAGGATGGTAATCTTCATTCTGCTGTTTTCAGTCCAACCACAGATCATGAGCTTGGTGGTGCTGGTAATGTCAATGTGAGTAAAGACCCAAAACTGAAATACAGAGGAAAGTATGTATAA

mRNA sequence

ATGTGGTATCTGTGTGTGTTCTATCACAGGCTATTGGATTACAGAAAGGCCGAGGTCGAATCTCTGGCCGAGTTGTTCGCAGAAAATGGCGACCAGTTGCAATGGAAGCTTCCCAAGCACCACCATCCCGATTCTCCATTCCATTTCGTCGACCTCTCTTCCGACGACCTAGCTCGGAAAATCGCAAATCGAAGTATACTTGTGAAGGGGATGTATGAACTATGGGGTGAAGGTGGTGACTACTTGGAGTTGGAGGAGTCTATTAGAAACTACCCTGATGAGCGGAAGTTGCCGTATTTACAACCCGGAAGCTCTTTCAAGATTTTTGTTGAGAGCTTTGGTAAGGCTATCAGTTTTGAGGAACAGACTGAACGTATTCGGGGGCTTGCTTACATTCCTTTCAAGGGTCAGGTTAATTTAAAAAACCCAGATCACAAGTTCTACCTGATGGAAATGGCTGATTATGGATTGAATAATGGACTTCCGCCAATTTCTCAAAGGAGTATTTTTTTTGGACGGGAGGTTGGTGGTGCTGATAGGAAGCTTATACCGACATATCAGTTAAAAAGTCGCACTTATCTTGGGCCAACTGCCATGGATGCTGAAATGGCTTTCTTAATGGCCAACCAAGCATTAGCAACAGCAGGGAAACTTGTCTATGACCCATTTGTTGGCACTGGGAGCGTTCTAGTTGCTGCAGCTCATTTTGGAGCAATGACAATGGGTGCAGACATTGACATTAGGGTGGTACGTGATGGTCGTGGCCCTGATTGTAATGTTTGGAGCAACTTCAAGCAGTATGGATTGCCACCGCCAATTGCACTGTTAAGAGCAGATAATAATCTTCCTCCCTGGCGTCCAGGATTGAAAGAGATATTTGATGCAATCATTTGTGACCCTCCTTATGGAGTGCGTGCTGGGGGGCGTAAATCTGGTGGTCGGAAATTGTTAAAAGGAACTGTTGCTCCATACACCGTTCCCGACGACAAGAGAACAGACCATATACCATCAACTGCAGCTTACAGCCTTGTTGAGTGTGTGCATGATTTACTTGACCTCGCCGCCAAGATGCTCGTAATAGGTGGCAGGCTTGTATTCTTCTACCCTGTGTTGAGGGAAGAAGATAGTGTGTTCAACCAATTTCCGGAGCATCCATGTTTTACTCTAATCAGTTCTTCCGAGCAGATATTGAGCTCTCGATACAGCCGGGTGTTGCTAACTATGGTCAAAAGAACTTCGTACACCGAAGAAATGGCGGAAGCAGCAAGATTAAAACACCAAGAATTCAGGGAGAACCATTTGAAATGGTTAGAGGATGGTAATCTTCATTCTGCTGTTTTCAGTCCAACCACAGATCATGAGCTTGGTGGTGCTGGTAATGTCAATGTGAGTAAAGACCCAAAACTGAAATACAGAGGAAAGTATGTATAA

Coding sequence (CDS)

ATGTGGTATCTGTGTGTGTTCTATCACAGGCTATTGGATTACAGAAAGGCCGAGGTCGAATCTCTGGCCGAGTTGTTCGCAGAAAATGGCGACCAGTTGCAATGGAAGCTTCCCAAGCACCACCATCCCGATTCTCCATTCCATTTCGTCGACCTCTCTTCCGACGACCTAGCTCGGAAAATCGCAAATCGAAGTATACTTGTGAAGGGGATGTATGAACTATGGGGTGAAGGTGGTGACTACTTGGAGTTGGAGGAGTCTATTAGAAACTACCCTGATGAGCGGAAGTTGCCGTATTTACAACCCGGAAGCTCTTTCAAGATTTTTGTTGAGAGCTTTGGTAAGGCTATCAGTTTTGAGGAACAGACTGAACGTATTCGGGGGCTTGCTTACATTCCTTTCAAGGGTCAGGTTAATTTAAAAAACCCAGATCACAAGTTCTACCTGATGGAAATGGCTGATTATGGATTGAATAATGGACTTCCGCCAATTTCTCAAAGGAGTATTTTTTTTGGACGGGAGGTTGGTGGTGCTGATAGGAAGCTTATACCGACATATCAGTTAAAAAGTCGCACTTATCTTGGGCCAACTGCCATGGATGCTGAAATGGCTTTCTTAATGGCCAACCAAGCATTAGCAACAGCAGGGAAACTTGTCTATGACCCATTTGTTGGCACTGGGAGCGTTCTAGTTGCTGCAGCTCATTTTGGAGCAATGACAATGGGTGCAGACATTGACATTAGGGTGGTACGTGATGGTCGTGGCCCTGATTGTAATGTTTGGAGCAACTTCAAGCAGTATGGATTGCCACCGCCAATTGCACTGTTAAGAGCAGATAATAATCTTCCTCCCTGGCGTCCAGGATTGAAAGAGATATTTGATGCAATCATTTGTGACCCTCCTTATGGAGTGCGTGCTGGGGGGCGTAAATCTGGTGGTCGGAAATTGTTAAAAGGAACTGTTGCTCCATACACCGTTCCCGACGACAAGAGAACAGACCATATACCATCAACTGCAGCTTACAGCCTTGTTGAGTGTGTGCATGATTTACTTGACCTCGCCGCCAAGATGCTCGTAATAGGTGGCAGGCTTGTATTCTTCTACCCTGTGTTGAGGGAAGAAGATAGTGTGTTCAACCAATTTCCGGAGCATCCATGTTTTACTCTAATCAGTTCTTCCGAGCAGATATTGAGCTCTCGATACAGCCGGGTGTTGCTAACTATGGTCAAAAGAACTTCGTACACCGAAGAAATGGCGGAAGCAGCAAGATTAAAACACCAAGAATTCAGGGAGAACCATTTGAAATGGTTAGAGGATGGTAATCTTCATTCTGCTGTTTTCAGTCCAACCACAGATCATGAGCTTGGTGGTGCTGGTAATGTCAATGTGAGTAAAGACCCAAAACTGAAATACAGAGGAAAGTATGTATAA

Protein sequence

MWYLCVFYHRLLDYRKAEVESLAELFAENGDQLQWKLPKHHHPDSPFHFVDLSSDDLARKIANRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKAISFEEQTERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHFGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAKMLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRTSYTEEMAEAARLKHQEFRENHLKWLEDGNLHSAVFSPTTDHELGGAGNVNVSKDPKLKYRGKYV
Homology
BLAST of Sed0027136 vs. NCBI nr
Match: XP_038888658.1 (tRNA (guanine(10)-N2)-methyltransferase homolog [Benincasa hispida])

HSP 1 Score: 905.2 bits (2338), Expect = 2.4e-259
Identity = 438/480 (91.25%), Postives = 454/480 (94.58%), Query Frame = 0

Query: 1   MWYLCVFYHRLLDYRKAEVESLAELFAENGD----QLQWKLPKHHHPDSPFHFVDLSSDD 60
           MWYLCVFYHRLLDYRKAEVESLAELF  NGD    +L+WKLP HHHPDSPFHFVDLSSDD
Sbjct: 1   MWYLCVFYHRLLDYRKAEVESLAELFGGNGDHKGEKLEWKLPLHHHPDSPFHFVDLSSDD 60

Query: 61  LARKIANRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKA 120
           LAR IANRSILVKGMYELWGEG DY ELEESIRNYP+ERK PYLQPGSSFKI VESFGKA
Sbjct: 61  LARNIANRSILVKGMYELWGEGSDYEELEESIRNYPEERKSPYLQPGSSFKISVESFGKA 120

Query: 121 ISFEEQTERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVG 180
           ISF+EQ ERI+GLAYIPFKGQV+LK+PDHKF LME AD+GLNNGLPPISQR IFFGREVG
Sbjct: 121 ISFQEQNERIQGLAYIPFKGQVSLKSPDHKFCLMETADHGLNNGLPPISQRRIFFGREVG 180

Query: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHF 240
           GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGS+LV AAHF
Sbjct: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSILVGAAHF 240

Query: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI 300
           GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPI LLRADNN PPWRPGLKE+FDAI
Sbjct: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIVLLRADNNRPPWRPGLKEMFDAI 300

Query: 301 ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAK 360
           ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAK
Sbjct: 301 ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAK 360

Query: 361 MLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRTSYTE 420
           MLV+GGRLVFFYPVLREED VFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR+SYTE
Sbjct: 361 MLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRSSYTE 420

Query: 421 EMAEAARLKHQEFRENHLKWLEDGNLHSAVFSPTTDHELGGAGNVNVSKDPKLKYRGKYV 477
           EMAEAARLKH+EFRENHLKWLEDGNLHSAVFSP+TD +L   GN N+SKDPK KYRGKYV
Sbjct: 421 EMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDSQLAVGGNCNLSKDPKPKYRGKYV 480

BLAST of Sed0027136 vs. NCBI nr
Match: XP_008447182.1 (PREDICTED: tRNA (guanine(10)-N2)-methyltransferase homolog [Cucumis melo])

HSP 1 Score: 902.1 bits (2330), Expect = 2.0e-258
Identity = 433/480 (90.21%), Postives = 456/480 (95.00%), Query Frame = 0

Query: 1   MWYLCVFYHRLLDYRKAEVESLAELFAENGD----QLQWKLPKHHHPDSPFHFVDLSSDD 60
           MWYLCVFYHRLLDYRK EVESLAELF ENGD    +L+WKLP HHHPDSPFHFVDLSSDD
Sbjct: 1   MWYLCVFYHRLLDYRKTEVESLAELFGENGDHKGKKLEWKLPLHHHPDSPFHFVDLSSDD 60

Query: 61  LARKIANRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKA 120
           +AR IANRSILVKGMYELWG+G DY ELEESIRN+P+ERK PYLQPGSSFKI VESFGKA
Sbjct: 61  IARSIANRSILVKGMYELWGQGSDYEELEESIRNFPEERKFPYLQPGSSFKISVESFGKA 120

Query: 121 ISFEEQTERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVG 180
           ISF+EQ ERI+GLAYIPFKGQVNLK+PDHKF L+E ADYGLNNGLPPI+QR IFFGREVG
Sbjct: 121 ISFQEQNERIQGLAYIPFKGQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVG 180

Query: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHF 240
           GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALAT+GKLV+DPFVGTGS+LV AAHF
Sbjct: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAHF 240

Query: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI 300
           GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI
Sbjct: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI 300

Query: 301 ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAK 360
           ICDPPYGVRAGGRKSGGRKLLKG VAPYTVPDDKRTDHIPSTA+YSLVECVHDLLDLAAK
Sbjct: 301 ICDPPYGVRAGGRKSGGRKLLKGIVAPYTVPDDKRTDHIPSTASYSLVECVHDLLDLAAK 360

Query: 361 MLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRTSYTE 420
           MLV+GGRLVFFYP+LREED VFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR+SYTE
Sbjct: 361 MLVMGGRLVFFYPMLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRSSYTE 420

Query: 421 EMAEAARLKHQEFRENHLKWLEDGNLHSAVFSPTTDHELGGAGNVNVSKDPKLKYRGKYV 477
           E+AEAARLKH+EFRENHLKWLEDGNLHSAVFSP+ + +LG AGN NVSKDPK KYRGKYV
Sbjct: 421 EIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSVNRQLGVAGNGNVSKDPKPKYRGKYV 480

BLAST of Sed0027136 vs. NCBI nr
Match: XP_031744574.1 (tRNA (guanine(10)-N2)-methyltransferase homolog [Cucumis sativus])

HSP 1 Score: 901.4 bits (2328), Expect = 3.4e-258
Identity = 434/480 (90.42%), Postives = 456/480 (95.00%), Query Frame = 0

Query: 1   MWYLCVFYHRLLDYRKAEVESLAELFAENGD----QLQWKLPKHHHPDSPFHFVDLSSDD 60
           MWYLCVFYHRLLDYRKAEVESLAELF ENGD    +L+WKLP HHHPDSPFHFVDLSSDD
Sbjct: 1   MWYLCVFYHRLLDYRKAEVESLAELFGENGDHKGKKLEWKLPLHHHPDSPFHFVDLSSDD 60

Query: 61  LARKIANRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKA 120
           +AR IANRSILVKGMYELWG+G DY ELEESIRN+P+ERK PYLQPGSSFKI VESFGKA
Sbjct: 61  IARNIANRSILVKGMYELWGQGSDYEELEESIRNFPEERKSPYLQPGSSFKISVESFGKA 120

Query: 121 ISFEEQTERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVG 180
           ISF+EQ ERI+GLAYIPFKGQVNLK+PDHKF L+E ADYGLNNGLPPI+QR IFFGREVG
Sbjct: 121 ISFQEQNERIQGLAYIPFKGQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVG 180

Query: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHF 240
           GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALAT+GKLV+DPFVGTGS+LV AAHF
Sbjct: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAHF 240

Query: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI 300
           GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI
Sbjct: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI 300

Query: 301 ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAK 360
           ICDPPYGVRAGGRKSGGRKLLKG VAPYTVPDDKRTDHIPSTA+YSLVECVHDLLDLAAK
Sbjct: 301 ICDPPYGVRAGGRKSGGRKLLKGIVAPYTVPDDKRTDHIPSTASYSLVECVHDLLDLAAK 360

Query: 361 MLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRTSYTE 420
           MLV+GGRLVFFYP+LREED VFNQFPEHPCFT ISSSEQILSSRYSRVLLTMVKR+SYTE
Sbjct: 361 MLVMGGRLVFFYPMLREEDCVFNQFPEHPCFTSISSSEQILSSRYSRVLLTMVKRSSYTE 420

Query: 421 EMAEAARLKHQEFRENHLKWLEDGNLHSAVFSPTTDHELGGAGNVNVSKDPKLKYRGKYV 477
           EMAEAARLKH+EFRENHLKWLEDGNLHSAVFSP+ + +LG AGN NVSKDPK KYRGKYV
Sbjct: 421 EMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSVNPQLGVAGNGNVSKDPKPKYRGKYV 480

BLAST of Sed0027136 vs. NCBI nr
Match: XP_022932935.1 (tRNA (guanine(10)-N2)-methyltransferase homolog [Cucurbita moschata] >XP_023511692.1 tRNA (guanine(10)-N2)-methyltransferase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 897.1 bits (2317), Expect = 6.5e-257
Identity = 431/480 (89.79%), Postives = 453/480 (94.38%), Query Frame = 0

Query: 1   MWYLCVFYHRLLDYRKAEVESLAELFAENGD----QLQWKLPKHHHPDSPFHFVDLSSDD 60
           MWYLCVFYHRLLDYRKAEVESLAELF ENGD    +L+WKLP HHHPDSPFHFVDLSSDD
Sbjct: 1   MWYLCVFYHRLLDYRKAEVESLAELFGENGDHKGGKLEWKLPLHHHPDSPFHFVDLSSDD 60

Query: 61  LARKIANRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKA 120
           +AR IANRSILVKGMYELWG G DY EL+ESIRN+P+ERK PYLQ GS+FKI VESFGKA
Sbjct: 61  IARSIANRSILVKGMYELWGVGSDYEELQESIRNFPEERKSPYLQQGSTFKISVESFGKA 120

Query: 121 ISFEEQTERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVG 180
           ISF+EQ ERI+GLAYIPFKGQVNL+NPDHKF LME ADYGLNNGLPP++QR+IFFGREVG
Sbjct: 121 ISFQEQNERIQGLAYIPFKGQVNLRNPDHKFSLMEAADYGLNNGLPPVAQRTIFFGREVG 180

Query: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHF 240
           GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALAT GKLVYDPFVGTGS+LV AAHF
Sbjct: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATPGKLVYDPFVGTGSILVGAAHF 240

Query: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI 300
           GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPI+LLRADNNLPPWRPGLKEIFDAI
Sbjct: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPISLLRADNNLPPWRPGLKEIFDAI 300

Query: 301 ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAK 360
           ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL+LAAK
Sbjct: 301 ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLNLAAK 360

Query: 361 MLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRTSYTE 420
           MLV+GGRLVFFYPVLREED VFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVK +SYTE
Sbjct: 361 MLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKHSSYTE 420

Query: 421 EMAEAARLKHQEFRENHLKWLEDGNLHSAVFSPTTDHELGGAGNVNVSKDPKLKYRGKYV 477
           EMAEAA+LKH+EFRENHLKWLEDGNLHSAVFSPT D +L  AGN N +KDPK KYRGKYV
Sbjct: 421 EMAEAAKLKHEEFRENHLKWLEDGNLHSAVFSPTIDPQLAVAGNCNATKDPKPKYRGKYV 480

BLAST of Sed0027136 vs. NCBI nr
Match: KAG7011460.1 (tRNA (guanine(10)-N2)-methyltransferase-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 891.7 bits (2303), Expect = 2.7e-255
Identity = 428/480 (89.17%), Postives = 452/480 (94.17%), Query Frame = 0

Query: 1   MWYLCVFYHRLLDYRKAEVESLAELFAENGD----QLQWKLPKHHHPDSPFHFVDLSSDD 60
           MWYLCVFYHRLLDYRKAEVESLAELF ENG+    +L+WKLP HHHPDSPFHFVDLSSDD
Sbjct: 1   MWYLCVFYHRLLDYRKAEVESLAELFGENGNHKGGKLEWKLPLHHHPDSPFHFVDLSSDD 60

Query: 61  LARKIANRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKA 120
           +AR IANRSILVKGMYELWG G DY EL+ESIRN+P+ERK PYLQ GS+FKI VESFGKA
Sbjct: 61  IARSIANRSILVKGMYELWGVGSDYEELQESIRNFPEERKSPYLQQGSTFKISVESFGKA 120

Query: 121 ISFEEQTERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVG 180
           ISF+EQ ERI+GLAYIPFKG VNL+NPDHKF LME ADYGLNNGLPP++QR+IFFGREVG
Sbjct: 121 ISFQEQNERIQGLAYIPFKGLVNLRNPDHKFSLMEAADYGLNNGLPPVAQRTIFFGREVG 180

Query: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHF 240
           GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALAT GKLVYDPFVGTGS+LV AAHF
Sbjct: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATPGKLVYDPFVGTGSILVGAAHF 240

Query: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI 300
           GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPI+LLRADNNLPPWRPGLKEIFDAI
Sbjct: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPISLLRADNNLPPWRPGLKEIFDAI 300

Query: 301 ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAK 360
           ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL+LAAK
Sbjct: 301 ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLNLAAK 360

Query: 361 MLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRTSYTE 420
           MLV+GGRLVFFYPVLREED VFNQFPEHPCFTL+SSSEQILSSRYSRVLLTMVK +SYTE
Sbjct: 361 MLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLLSSSEQILSSRYSRVLLTMVKHSSYTE 420

Query: 421 EMAEAARLKHQEFRENHLKWLEDGNLHSAVFSPTTDHELGGAGNVNVSKDPKLKYRGKYV 477
           EMAEAA+LKH+EFRENHLKWLEDGNLHSAVFSPT D +L  AGN N +KDPK KYRGKYV
Sbjct: 421 EMAEAAKLKHEEFRENHLKWLEDGNLHSAVFSPTIDPQLAVAGNCNATKDPKPKYRGKYV 480

BLAST of Sed0027136 vs. ExPASy Swiss-Prot
Match: Q6NS23 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Xenopus laevis OX=8355 GN=trmt11 PE=2 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 5.8e-83
Identity = 175/456 (38.38%), Postives = 263/456 (57.68%), Query Frame = 0

Query: 3   YLCVFYHRLLDYRKAEVESLAELFAENGDQLQWKLPKHHHPDSPFHFVDLSSDDLARKIA 62
           YL +      ++R  E++SL  L+  + + LQ       H  SPF  ++L S+++ARK+ 
Sbjct: 14  YLLLLAQENCEFRLPEIKSLLSLYGGHFNNLQ-----DEHGKSPFCILNLPSEEMARKLM 73

Query: 63  NRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKAISFEEQ 122
            R++  K ++ELWG G  ++E ++S+ +YP E  + YLQP S++KI + SF K ++ +E+
Sbjct: 74  KRTVCAKSVFELWGHGKTFMEFQQSVLSYPLENMMSYLQPNSTYKIIIHSFNKTLTQKEK 133

Query: 123 TERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVGGADRKL 182
            E+I  + +IPF+G+VNL+N ++ FYL+E  DYG +    P     IFFGR +    R+L
Sbjct: 134 LEKINTMEFIPFQGKVNLQNAENIFYLLE--DYGSDPNKAPNEPFEIFFGRWIADGQREL 193

Query: 183 IPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHFGAMTMG 242
           I +Y +K R ++G T+MDA ++F+MAN A      +V+DPFVGTG +LV++AHFGA   G
Sbjct: 194 INSYSVKKRHFIGNTSMDAGLSFIMANHARVKPNDVVFDPFVGTGGLLVSSAHFGAYVCG 253

Query: 243 ADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPGLKE 302
            +ID   V          +  RGPD N+ +N +QYGL    + +L +D + P WR     
Sbjct: 254 TEIDYNTVHGLGKATRMNQKWRGPDENIRANLRQYGLEKYYLDVLVSDASKPVWRKA--P 313

Query: 303 IFDAIICDPPYGVRAGGRKSGGRK-LLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDL 362
           +FDAII DPPYG+R   RK+G +K ++K  + P          H+P    Y L +   DL
Sbjct: 314 LFDAIITDPPYGIRESTRKTGTQKEIIKTELFP--------ESHVPVQLNYHLSDIFSDL 373

Query: 363 LDLAAKMLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVK 422
              A++ LV GGRLV++ PV R E +     P HPC  LIS+ EQ+LSS  SR L+TM K
Sbjct: 374 FAFASEFLVTGGRLVYWLPVYRPEYTE-EVLPRHPCLKLISNCEQMLSSHTSRRLITMEK 433

Query: 423 RTSYTEE-----MAEAARLK---HQEFRENHLKWLE 439
              + E+     + E   L    H  FRE +   L+
Sbjct: 434 VKEFEEKYQYAHLGEGQNLPYKGHNSFREKYFSGLK 451

BLAST of Sed0027136 vs. ExPASy Swiss-Prot
Match: Q5R962 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Pongo abelii OX=9601 GN=TRMT11 PE=2 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 4.1e-81
Identity = 175/451 (38.80%), Postives = 257/451 (56.98%), Query Frame = 0

Query: 3   YLCVFYHRLLDYRKAEVESLAELFAENGDQLQWKLPKHHHPDSPFHFVDLSSDDLARKIA 62
           YL +     L++R  E++SL  LF       Q    K     SPF  + + S+D+AR + 
Sbjct: 10  YLLLMAQEHLEFRLPEIKSLLSLFGGQFGSSQETFGK-----SPFWILSIPSEDIARNLM 69

Query: 63  NRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKAISFEEQ 122
            R++  K ++ELWG G    EL  S++NYP E+ +P+L   S++KI + +F K ++ EE+
Sbjct: 70  KRTVCAKSIFELWGHGQSPEELYSSLKNYPVEKMVPFLHSDSTYKIKIHTFNKTLTQEEK 129

Query: 123 TERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVGGADRKL 182
            +RI  L ++PF+G+VNLK P H F ++E  DYGL+    P +  +I+FGR +    R+L
Sbjct: 130 IKRIDALEFLPFEGKVNLKKPQHVFSVLE--DYGLDPNCIPENPHNIYFGRWIADGQREL 189

Query: 183 IPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHFGAMTMG 242
           I +Y +K R ++G T+MDA ++F+MAN        +V+DPFVGTG +L+A AHFGA   G
Sbjct: 190 IESYSVKKRHFIGNTSMDAGLSFIMANHGKVKENDIVFDPFVGTGGLLIACAHFGAYVYG 249

Query: 243 ADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPGLKE 302
            DID   V          +  RGPD N+ +N +QYGL    + +L +D + P WR G   
Sbjct: 250 TDIDYNTVHGLGKATRKNQKWRGPDENIRANLRQYGLEKYYLDVLVSDASKPSWRKG--T 309

Query: 303 IFDAIICDPPYGVRAGGRKSGGRK-LLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDL 362
            FDAII DPPYG+R   R++G +K + KG       P+     H+P + +Y L +   DL
Sbjct: 310 YFDAIITDPPYGIRESTRRTGSQKEIPKGIEKWEKCPE----SHVPVSLSYHLSDMFLDL 369

Query: 363 LDLAAKMLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVK 422
           L+ AA+ LV+GGRLV++ PV   E +     P HPC  LIS+ EQ LSS  SR L+TM K
Sbjct: 370 LNFAAETLVLGGRLVYWLPVYTPEYTE-EMVPWHPCLELISNCEQKLSSHTSRRLITMEK 429

Query: 423 ------RTSYTEEMAE--AARLKHQEFRENH 434
                 R  Y+  +++       H  FRE +
Sbjct: 430 VKKFENRDQYSHLLSDHFLPYQGHNSFREKY 446

BLAST of Sed0027136 vs. ExPASy Swiss-Prot
Match: Q7Z4G4 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Homo sapiens OX=9606 GN=TRMT11 PE=1 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.2e-80
Identity = 173/451 (38.36%), Postives = 259/451 (57.43%), Query Frame = 0

Query: 3   YLCVFYHRLLDYRKAEVESLAELFAENGDQLQWKLPKHHHPDSPFHFVDLSSDDLARKIA 62
           YL +     L++R  E++SL  LF       Q+   +  +  SPF  + + S+D+AR + 
Sbjct: 10  YLLLMAQEHLEFRLPEIKSLLLLFGG-----QFASSQETYGKSPFWILSIPSEDIARNLM 69

Query: 63  NRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKAISFEEQ 122
            R++  K ++ELWG G    EL  S++NYP E+ +P+L   S++KI + +F K ++ EE+
Sbjct: 70  KRTVCAKSIFELWGHGQSPEELYSSLKNYPVEKMVPFLHSDSTYKIKIHTFNKTLTQEEK 129

Query: 123 TERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVGGADRKL 182
            +RI  L ++PF+G+VNLK P H F ++E  DYGL+    P +  +I+FGR +    R+L
Sbjct: 130 IKRIDALEFLPFEGKVNLKKPQHVFSVLE--DYGLDPNCIPENPHNIYFGRWIADGQREL 189

Query: 183 IPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHFGAMTMG 242
           I +Y +K R ++G T+MDA ++F+MAN        +V+DPFVGTG +L+A AHFGA   G
Sbjct: 190 IESYSVKKRHFIGNTSMDAGLSFIMANHGKVKENDIVFDPFVGTGGLLIACAHFGAYVYG 249

Query: 243 ADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPGLKE 302
            DID   V          +  RGPD N+ +N +QYGL    + +L +D + P WR G   
Sbjct: 250 TDIDYNTVHGLGKATRKNQKWRGPDENIRANLRQYGLEKYYLDVLVSDASKPSWRKG--T 309

Query: 303 IFDAIICDPPYGVRAGGRKSGGRK-LLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDL 362
            FDAII DPPYG+R   R++G +K + KG       P+     H+P + +Y L +   DL
Sbjct: 310 YFDAIITDPPYGIRESTRRTGSQKEIPKGIEKWEKCPE----SHVPVSLSYHLSDMFLDL 369

Query: 363 LDLAAKMLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVK 422
           L+ AA+ LV+GGRLV++ PV   E +     P HPC  L+S+ EQ LSS  SR L+TM K
Sbjct: 370 LNFAAETLVLGGRLVYWLPVYTPEYTE-EMVPWHPCLELVSNCEQKLSSHTSRRLITMEK 429

Query: 423 ------RTSYTEEMAE--AARLKHQEFRENH 434
                 R  Y+  +++       H  FRE +
Sbjct: 430 VKKFENRDQYSHLLSDHFLPYQGHNSFREKY 446

BLAST of Sed0027136 vs. ExPASy Swiss-Prot
Match: Q05B63 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Bos taurus OX=9913 GN=TRMT11 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.1e-80
Identity = 171/450 (38.00%), Postives = 259/450 (57.56%), Query Frame = 0

Query: 3   YLCVFYHRLLDYRKAEVESLAELFAENGDQLQWKLPKHHHPDSPFHFVDLSSDDLARKIA 62
           YL +     L++R  E++SL  LF       Q+   +  +  SPF  + + S+D+AR + 
Sbjct: 10  YLLLMAQEHLEFRLPEIKSLLSLFGG-----QFISSQETYGKSPFWILSIPSEDIARNLM 69

Query: 63  NRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKAISFEEQ 122
            R++  K ++ELWG G    EL  S++NYP E+ +P+L   S++KI + +F K ++ EE+
Sbjct: 70  KRTVCAKSIFELWGHGKCPEELYSSLKNYPVEKMVPFLHSDSTYKIKIHTFNKTLTQEEK 129

Query: 123 TERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVGGADRKL 182
            +RI  L ++PF+G+VNLK P H F ++E  DYGL+    P +  +I+FGR +    R+L
Sbjct: 130 VKRIDALEFLPFEGKVNLKKPQHVFSILE--DYGLDPNHIPENPHNIYFGRWIADGQREL 189

Query: 183 IPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHFGAMTMG 242
           I +Y +K R ++G T+MDA ++F+MAN        +V+DPFVGTG +L+A+AHFGA   G
Sbjct: 190 IESYSVKKRHFIGNTSMDAGLSFIMANHGKVKKNDIVFDPFVGTGGLLIASAHFGAYVYG 249

Query: 243 ADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPGLKE 302
            DID   V          +  RGPD N+ +N +QYGL    + +L +D + P WR G   
Sbjct: 250 TDIDYNTVHGLGKASRKNQKWRGPDENIRANLRQYGLEKYYLDVLVSDASKPSWRKG--T 309

Query: 303 IFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 362
            FDAII DPPYG+R   R++G +K +   +      +     H+P + +Y L +   DLL
Sbjct: 310 YFDAIITDPPYGIRESTRRTGSQKEIPKGI------EKCPESHVPVSLSYHLSDMFFDLL 369

Query: 363 DLAAKMLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVK- 422
           + +A+ LV+GGRLV++ PV   E +     P HPC  LIS+ EQ LSS  SR L+TM K 
Sbjct: 370 NFSAETLVLGGRLVYWLPVYTPEYTE-EMVPWHPCLKLISNCEQKLSSHTSRRLITMEKV 429

Query: 423 -----RTSYTEEMAEAARL--KHQEFRENH 434
                R  Y+  +++   L   H  FRE +
Sbjct: 430 KEFENRDQYSHLLSDHFLLYQGHNSFREKY 443

BLAST of Sed0027136 vs. ExPASy Swiss-Prot
Match: Q7TNK6 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Rattus norvegicus OX=10116 GN=Trmt11 PE=2 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 3.5e-80
Identity = 172/452 (38.05%), Postives = 260/452 (57.52%), Query Frame = 0

Query: 3   YLCVFYHRLLDYRKAEVESLAELFAENGDQLQWKLPKHHHPDSPFHFVDLSSDDLARKIA 62
           YL +     L++R  E++SL  +        Q+   +  +  SPF  +++ S+D+AR + 
Sbjct: 10  YLLLMAQEHLEFRLPEIKSLLSVIGG-----QFTNSQETYGKSPFWILNIPSEDIARNLM 69

Query: 63  NRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKAISFEEQ 122
            R++  K ++ELWG G    EL  S+++YP E+ +P+L   S++KI + +F K ++ EE+
Sbjct: 70  KRTVCAKSLFELWGHGKSPEELYSSLKSYPVEKMVPFLHSDSTYKIKIHTFNKTLTQEEK 129

Query: 123 TERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVGGADRKL 182
            +RI  L ++PF+G+VNLK P H F ++E  DYGL+    P +  +I+FGR +    R+L
Sbjct: 130 VKRIDALEFLPFEGKVNLKKPQHVFSILE--DYGLDPNCIPENPHNIYFGRWIADGQREL 189

Query: 183 IPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHFGAMTMG 242
           I +Y +K R ++G T+MDA ++F+MAN A      LV+DPFVGTG +L+A+AHFGA   G
Sbjct: 190 IESYSVKKRHFIGNTSMDAGLSFIMANHAKVKENDLVFDPFVGTGGLLIASAHFGAYVCG 249

Query: 243 ADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPGLKE 302
            DID   V          +  RGPD N+ +N +QYGL    + +L +D + P WR G   
Sbjct: 250 TDIDYNTVHGLGKASRKNQKWRGPDENIRANLRQYGLEKFYLDVLVSDASKPSWRKG--T 309

Query: 303 IFDAIICDPPYGVRAGGRKSGGRK-LLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDL 362
            FDAII DPPYG+R   R++G +K +LKG        +     H+P +  Y L +   DL
Sbjct: 310 YFDAIITDPPYGIRESTRRTGSQKEILKGI-------EKCPESHVPVSLTYHLSDMFLDL 369

Query: 363 LDLAAKMLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVK 422
           ++ AA+ LV+GGRLV++ PV   E +     P HPC  L+S+ EQ LSS  +R L+TM K
Sbjct: 370 INFAAETLVLGGRLVYWLPVYTPEYTE-KMVPWHPCLRLVSNCEQKLSSHTARRLITMEK 429

Query: 423 RTSYTEEMAEAARL---------KHQEFRENH 434
              + E   E +RL          H  FRE +
Sbjct: 430 VKEF-ENPDEYSRLLSDHFLPYQGHNSFREKY 443

BLAST of Sed0027136 vs. ExPASy TrEMBL
Match: A0A1S3BG98 (tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucumis melo OX=3656 GN=LOC103489693 PE=4 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 9.7e-259
Identity = 433/480 (90.21%), Postives = 456/480 (95.00%), Query Frame = 0

Query: 1   MWYLCVFYHRLLDYRKAEVESLAELFAENGD----QLQWKLPKHHHPDSPFHFVDLSSDD 60
           MWYLCVFYHRLLDYRK EVESLAELF ENGD    +L+WKLP HHHPDSPFHFVDLSSDD
Sbjct: 1   MWYLCVFYHRLLDYRKTEVESLAELFGENGDHKGKKLEWKLPLHHHPDSPFHFVDLSSDD 60

Query: 61  LARKIANRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKA 120
           +AR IANRSILVKGMYELWG+G DY ELEESIRN+P+ERK PYLQPGSSFKI VESFGKA
Sbjct: 61  IARSIANRSILVKGMYELWGQGSDYEELEESIRNFPEERKFPYLQPGSSFKISVESFGKA 120

Query: 121 ISFEEQTERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVG 180
           ISF+EQ ERI+GLAYIPFKGQVNLK+PDHKF L+E ADYGLNNGLPPI+QR IFFGREVG
Sbjct: 121 ISFQEQNERIQGLAYIPFKGQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVG 180

Query: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHF 240
           GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALAT+GKLV+DPFVGTGS+LV AAHF
Sbjct: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAHF 240

Query: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI 300
           GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI
Sbjct: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI 300

Query: 301 ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAK 360
           ICDPPYGVRAGGRKSGGRKLLKG VAPYTVPDDKRTDHIPSTA+YSLVECVHDLLDLAAK
Sbjct: 301 ICDPPYGVRAGGRKSGGRKLLKGIVAPYTVPDDKRTDHIPSTASYSLVECVHDLLDLAAK 360

Query: 361 MLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRTSYTE 420
           MLV+GGRLVFFYP+LREED VFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR+SYTE
Sbjct: 361 MLVMGGRLVFFYPMLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRSSYTE 420

Query: 421 EMAEAARLKHQEFRENHLKWLEDGNLHSAVFSPTTDHELGGAGNVNVSKDPKLKYRGKYV 477
           E+AEAARLKH+EFRENHLKWLEDGNLHSAVFSP+ + +LG AGN NVSKDPK KYRGKYV
Sbjct: 421 EIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSVNRQLGVAGNGNVSKDPKPKYRGKYV 480

BLAST of Sed0027136 vs. ExPASy TrEMBL
Match: A0A6J1F3J0 (tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucurbita moschata OX=3662 GN=LOC111439552 PE=4 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.1e-257
Identity = 431/480 (89.79%), Postives = 453/480 (94.38%), Query Frame = 0

Query: 1   MWYLCVFYHRLLDYRKAEVESLAELFAENGD----QLQWKLPKHHHPDSPFHFVDLSSDD 60
           MWYLCVFYHRLLDYRKAEVESLAELF ENGD    +L+WKLP HHHPDSPFHFVDLSSDD
Sbjct: 1   MWYLCVFYHRLLDYRKAEVESLAELFGENGDHKGGKLEWKLPLHHHPDSPFHFVDLSSDD 60

Query: 61  LARKIANRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKA 120
           +AR IANRSILVKGMYELWG G DY EL+ESIRN+P+ERK PYLQ GS+FKI VESFGKA
Sbjct: 61  IARSIANRSILVKGMYELWGVGSDYEELQESIRNFPEERKSPYLQQGSTFKISVESFGKA 120

Query: 121 ISFEEQTERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVG 180
           ISF+EQ ERI+GLAYIPFKGQVNL+NPDHKF LME ADYGLNNGLPP++QR+IFFGREVG
Sbjct: 121 ISFQEQNERIQGLAYIPFKGQVNLRNPDHKFSLMEAADYGLNNGLPPVAQRTIFFGREVG 180

Query: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHF 240
           GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALAT GKLVYDPFVGTGS+LV AAHF
Sbjct: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATPGKLVYDPFVGTGSILVGAAHF 240

Query: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI 300
           GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPI+LLRADNNLPPWRPGLKEIFDAI
Sbjct: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPISLLRADNNLPPWRPGLKEIFDAI 300

Query: 301 ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAK 360
           ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL+LAAK
Sbjct: 301 ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLNLAAK 360

Query: 361 MLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRTSYTE 420
           MLV+GGRLVFFYPVLREED VFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVK +SYTE
Sbjct: 361 MLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKHSSYTE 420

Query: 421 EMAEAARLKHQEFRENHLKWLEDGNLHSAVFSPTTDHELGGAGNVNVSKDPKLKYRGKYV 477
           EMAEAA+LKH+EFRENHLKWLEDGNLHSAVFSPT D +L  AGN N +KDPK KYRGKYV
Sbjct: 421 EMAEAAKLKHEEFRENHLKWLEDGNLHSAVFSPTIDPQLAVAGNCNATKDPKPKYRGKYV 480

BLAST of Sed0027136 vs. ExPASy TrEMBL
Match: A0A6J1ICG5 (tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucurbita maxima OX=3661 GN=LOC111472466 PE=4 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.5e-254
Identity = 427/480 (88.96%), Postives = 449/480 (93.54%), Query Frame = 0

Query: 1   MWYLCVFYHRLLDYRKAEVESLAELFAENGD----QLQWKLPKHHHPDSPFHFVDLSSDD 60
           MWYLCVFYHRLLDYRKAEVESLAELF ENGD    +L+WKLP HHHPDSPFHFVDLSSDD
Sbjct: 1   MWYLCVFYHRLLDYRKAEVESLAELFGENGDHKGGKLEWKLPLHHHPDSPFHFVDLSSDD 60

Query: 61  LARKIANRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKA 120
           +AR IANRSILVKGMYELWG G DY EL+ESI+ +P+ERK PYL  GS+FKI VESFGKA
Sbjct: 61  IARNIANRSILVKGMYELWGVGSDYEELQESIKTFPEERKSPYLLQGSTFKISVESFGKA 120

Query: 121 ISFEEQTERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVG 180
           ISF+EQ ERI+GLAYIPFKGQVNL+NPDHKF LME ADYGLNNGLPP++QR+IFFGREVG
Sbjct: 121 ISFQEQNERIQGLAYIPFKGQVNLRNPDHKFSLMEAADYGLNNGLPPVAQRTIFFGREVG 180

Query: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHF 240
           GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALAT GKLVYDPFVGTGS+LV AAHF
Sbjct: 181 GADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATPGKLVYDPFVGTGSILVGAAHF 240

Query: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI 300
           GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI
Sbjct: 241 GAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAI 300

Query: 301 ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAK 360
           ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL+LAAK
Sbjct: 301 ICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLNLAAK 360

Query: 361 MLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRTSYTE 420
           MLV+GGRLVFFYPVLREED VFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVK +SYT+
Sbjct: 361 MLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKHSSYTK 420

Query: 421 EMAEAARLKHQEFRENHLKWLEDGNLHSAVFSPTTDHELGGAGNVNVSKDPKLKYRGKYV 477
           EMAEAA+LKH+EFRENHLKWLEDGNLHSAVF PT D  L  AGN N +KDPK KYRGKYV
Sbjct: 421 EMAEAAKLKHEEFRENHLKWLEDGNLHSAVFGPTIDPRLTVAGNCNATKDPKPKYRGKYV 480

BLAST of Sed0027136 vs. ExPASy TrEMBL
Match: A0A6J1D5Q5 (tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Momordica charantia OX=3673 GN=LOC111017553 PE=4 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 7.2e-254
Identity = 433/483 (89.65%), Postives = 451/483 (93.37%), Query Frame = 0

Query: 1   MWYLCVFYHRLLDYRKAEVESLAEL----FAENGD---QLQWKLPKHHHPDSPFHFVDLS 60
           MW+LCVFYHRLLDYRKAEVESLAEL    F ENGD   +L+W+LP HHHPDSPFHFV+L 
Sbjct: 1   MWFLCVFYHRLLDYRKAEVESLAELFGGPFGENGDAKAKLEWRLPLHHHPDSPFHFVNLP 60

Query: 61  SDDLARKIANRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESF 120
           SDDLAR+IA RSILVKGMYELWGEG D  ELEESI+NYPDERK PYLQPGSSFKI V+SF
Sbjct: 61  SDDLARRIATRSILVKGMYELWGEGSDDKELEESIKNYPDERKTPYLQPGSSFKITVDSF 120

Query: 121 GKAISFEEQTERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGR 180
           GKAISF+EQ ERIRGLAYIPFKGQVNLKNPDHKF LMEMADYGLNNGLPPI+QRSIFFGR
Sbjct: 121 GKAISFQEQNERIRGLAYIPFKGQVNLKNPDHKFCLMEMADYGLNNGLPPIAQRSIFFGR 180

Query: 181 EVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAA 240
           EVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGS+LVAA
Sbjct: 181 EVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSILVAA 240

Query: 241 AHFGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIF 300
           AHFGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLP PIALLRADNNLPPWRPGLKEIF
Sbjct: 241 AHFGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPSPIALLRADNNLPPWRPGLKEIF 300

Query: 301 DAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDL 360
           DAIICDPPYGVRAGGRKSGGRKLLKG VAPYT PDDKRTDHIPSTAAYSLVECVHDLLDL
Sbjct: 301 DAIICDPPYGVRAGGRKSGGRKLLKGIVAPYTXPDDKRTDHIPSTAAYSLVECVHDLLDL 360

Query: 361 AAKMLVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRTS 420
           AA+MLV+GGRLVFFYPVLREED  FNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR S
Sbjct: 361 AARMLVMGGRLVFFYPVLREEDCAFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRNS 420

Query: 421 YTEEMAEAARLKHQEFRENHLKWLEDGNLHSAVFSPTTDHELGGAGNVNVSKDPKLKYRG 477
           YTEE+AEA+RLKH+EFRENHLKWLEDGNLHSAVFSP  D EL  AGN +V +DPK KYRG
Sbjct: 421 YTEELAEASRLKHEEFRENHLKWLEDGNLHSAVFSP-ADPELAVAGNGSVGRDPKPKYRG 480

BLAST of Sed0027136 vs. ExPASy TrEMBL
Match: A0A5A7TF32 (tRNA (Guanine(10)-N2)-methyltransferase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67G003460 PE=4 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 9.5e-238
Identity = 402/452 (88.94%), Postives = 426/452 (94.25%), Query Frame = 0

Query: 16  KAEVESLAELFAENGD----QLQWKLPKHHHPDSPFHFVDLSSDDLARKIANRSILVKGM 75
           K EVESLAELF ENGD    +L+WKLP HHHPDSPFHFVDLSSDD+AR IANR   +KGM
Sbjct: 130 KTEVESLAELFGENGDHKGKKLEWKLPLHHHPDSPFHFVDLSSDDIARSIANR---MKGM 189

Query: 76  YELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKAISFEEQTERIRGLAY 135
           YELWG+G DY ELEESIRN+P+ERK PYLQPGSSFKI VESFGKAISF+EQ ERI+GLAY
Sbjct: 190 YELWGQGSDYEELEESIRNFPEERKFPYLQPGSSFKISVESFGKAISFQEQNERIQGLAY 249

Query: 136 IPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVGGADRKLIPTYQLKSR 195
           IPFKGQVNLK+PDHKF L+E ADYGLNNGLPPI+QR IFFGREVGGADRKLIPTYQLKSR
Sbjct: 250 IPFKGQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSR 309

Query: 196 TYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHFGAMTMGADIDIRVVR 255
           TYLGPTAMDAEMAFLMANQALAT+GKLV+DPFVGTGS+LV AAHFGAMTMGADIDIRVVR
Sbjct: 310 TYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAHFGAMTMGADIDIRVVR 369

Query: 256 DGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAIICDPPYGVRAGGRKS 315
           DGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAIICDPPYGVRAGGRKS
Sbjct: 370 DGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAIICDPPYGVRAGGRKS 429

Query: 316 GGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAKMLVIGGRLVFFYPVL 375
           GGRKLLKG VAPYTVPDDKRTDHIPSTA+YSLVECVHDLLDLAAKMLV+GGRLVFFYP+L
Sbjct: 430 GGRKLLKGIVAPYTVPDDKRTDHIPSTASYSLVECVHDLLDLAAKMLVMGGRLVFFYPML 489

Query: 376 REEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRTSYTEEMAEAARLKHQEFRE 435
           REED VFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKR+SYTEE+AEAARLKH+EFRE
Sbjct: 490 REEDCVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRSSYTEEIAEAARLKHEEFRE 549

Query: 436 NHLKWLEDGNLHSAVFSPTTDHELGGAGNVNV 464
           NHLKWLEDGNLHSAVFSP+ + +LG AGN NV
Sbjct: 550 NHLKWLEDGNLHSAVFSPSVNRQLGVAGNGNV 578

BLAST of Sed0027136 vs. TAIR 10
Match: AT3G26410.1 (methyltransferases;nucleic acid binding )

HSP 1 Score: 746.5 bits (1926), Expect = 1.3e-215
Identity = 358/479 (74.74%), Postives = 410/479 (85.59%), Query Frame = 0

Query: 1   MWYLCVFYHRLLDYRKAEVESLAELFAE---NGDQLQWKLPKHHHPDSPFHFVDLSSDDL 60
           MW+LCVFYHRLLD+RK EVE+LAELF E     + LQW+LP++HH D+PFHFV LSS+++
Sbjct: 1   MWFLCVFYHRLLDFRKPEVEALAELFGEEIAENESLQWRLPENHHNDTPFHFVQLSSEEI 60

Query: 61  ARKIANRSILVKGMYELWGEGGDYLELEESIRNYPDERKLPYLQPGSSFKIFVESFGKAI 120
           AR IA RSILVKGMYELWGEG  Y EL++SI++YPD RKLP+L   S+F+I VE+FGKA+
Sbjct: 61  ARNIAKRSILVKGMYELWGEGTCYEELKDSIQSYPDSRKLPFLTSDSTFRISVETFGKAL 120

Query: 121 SFEEQTERIRGLAYIPFKGQVNLKNPDHKFYLMEMADYGLNNGLPPISQRSIFFGREVGG 180
           +F+EQ +RI+   YIPF+G+VNLKNPDH F+LMEM +   NNGL PI QR IFFGREVG 
Sbjct: 121 TFDEQKDRIQSFTYIPFEGRVNLKNPDHNFFLMEMIESEENNGLQPILQRRIFFGREVGF 180

Query: 181 ADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATAGKLVYDPFVGTGSVLVAAAHFG 240
           ADRKL+PT+QLKSRTYLGPTAMDAEMAFLMANQA AT+GKLVYDPFVGTGS+LV+AA FG
Sbjct: 181 ADRKLLPTFQLKSRTYLGPTAMDAEMAFLMANQAKATSGKLVYDPFVGTGSILVSAARFG 240

Query: 241 AMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEIFDAII 300
           AMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLP P+ALLR DNN+PPWR GLKEIFDAII
Sbjct: 241 AMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPMPVALLRMDNNVPPWRSGLKEIFDAII 300

Query: 301 CDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAAKM 360
           CDPPYGVRAGGRKSGGRK+L+GTV PYTVPDDKRTDHIPST AYSLVECVHDLL LAA+M
Sbjct: 301 CDPPYGVRAGGRKSGGRKILRGTVDPYTVPDDKRTDHIPSTGAYSLVECVHDLLHLAARM 360

Query: 361 LVIGGRLVFFYPVLREEDSVFNQFPEHPCFTLISSSEQILSSRYSRVLLTMVKRTSYTEE 420
           LV+ GRLVFF+PVLR+E     +FPEHPCF L++ SEQILSSRYSRVLLTMVK   Y+EE
Sbjct: 361 LVMKGRLVFFFPVLRDETGSEVKFPEHPCFKLVAVSEQILSSRYSRVLLTMVKVEPYSEE 420

Query: 421 MAEAARLKHQEFRENHLKWLEDGNLHSAVFSPTTDHELGGAGNVNVSKDPKLKYRGKYV 477
           + EAARL H EFRENHLKWLEDGN+HS++F P    ++    +    KDPK KYRGKYV
Sbjct: 421 VEEAARLMHLEFRENHLKWLEDGNIHSSIFKPIDSSQID--TDSKAFKDPKPKYRGKYV 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888658.12.4e-25991.25tRNA (guanine(10)-N2)-methyltransferase homolog [Benincasa hispida][more]
XP_008447182.12.0e-25890.21PREDICTED: tRNA (guanine(10)-N2)-methyltransferase homolog [Cucumis melo][more]
XP_031744574.13.4e-25890.42tRNA (guanine(10)-N2)-methyltransferase homolog [Cucumis sativus][more]
XP_022932935.16.5e-25789.79tRNA (guanine(10)-N2)-methyltransferase homolog [Cucurbita moschata] >XP_0235116... [more]
KAG7011460.12.7e-25589.17tRNA (guanine(10)-N2)-methyltransferase-like protein [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
Q6NS235.8e-8338.38tRNA (guanine(10)-N2)-methyltransferase homolog OS=Xenopus laevis OX=8355 GN=trm... [more]
Q5R9624.1e-8138.80tRNA (guanine(10)-N2)-methyltransferase homolog OS=Pongo abelii OX=9601 GN=TRMT1... [more]
Q7Z4G41.2e-8038.36tRNA (guanine(10)-N2)-methyltransferase homolog OS=Homo sapiens OX=9606 GN=TRMT1... [more]
Q05B632.1e-8038.00tRNA (guanine(10)-N2)-methyltransferase homolog OS=Bos taurus OX=9913 GN=TRMT11 ... [more]
Q7TNK63.5e-8038.05tRNA (guanine(10)-N2)-methyltransferase homolog OS=Rattus norvegicus OX=10116 GN... [more]
Match NameE-valueIdentityDescription
A0A1S3BG989.7e-25990.21tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1F3J03.1e-25789.79tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1ICG52.5e-25488.96tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1D5Q57.2e-25489.65tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Momordica charantia OX=3673 G... [more]
A0A5A7TF329.5e-23888.94tRNA (Guanine(10)-N2)-methyltransferase-like protein OS=Cucumis melo var. makuwa... [more]
Match NameE-valueIdentityDescription
AT3G26410.11.3e-21574.74methyltransferases;nucleic acid binding [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000241Putative RNA methylase domainPFAMPF01170UPF0020coord: 190..312
e-value: 1.7E-10
score: 40.9
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 189..374
e-value: 1.1E-25
score: 92.2
NoneNo IPR availablePIRSFPIRSF017259tRNA_Mtase_TRM11coord: 1..453
e-value: 5.7E-140
score: 465.0
NoneNo IPR availablePANTHERPTHR13370RNA METHYLASE-RELATEDcoord: 1..456
NoneNo IPR availablePANTHERPTHR13370:SF19SUBFAMILY NOT NAMEDcoord: 1..456
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 219..302
e-value: 0.00808902
score: 34.3279
IPR002052DNA methylase, N-6 adenine-specific, conserved sitePROSITEPS00092N6_MTASEcoord: 296..302
IPR016691tRNA guanosine-2'-O-methyltransferase, TRM11PROSITEPS51627SAM_MT_TRM11coord: 1..476
score: 136.301498
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 202..413

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0027136.1Sed0027136.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
biological_process GO:0032259 methylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004809 tRNA (guanine-N2-)-methyltransferase activity
molecular_function GO:0008168 methyltransferase activity