Sed0026888 (gene) Chayote v1

Overview
NameSed0026888
Typegene
OrganismSechium edule (Chayote v1)
DescriptionPhosphoglycerate kinase family protein
LocationLG01: 5709336 .. 5712469 (+)
RNA-Seq ExpressionSed0026888
SyntenySed0026888
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TACCAATTCAATCTCTTTCTCCATTCATTCCTCACAACAACAAAAACCCAAATCGCCAAAACAACTTGGGTTCGCCCATAGCTTCATCAATGGCGTCTGCAGCTTCCCCCACCACATTCCATCTTCTTCCCACACCATCCTCTTCCACTTCATCAACCCGCCCTTCCCTTCTTCAATTTCCCCCCACCGCCGCCTCCTCGATCCGCCTCCGCTCCGCCGCCGCAGACCCACTTTTCTCCGCCCACGTGGCTTCCAAGATTCGGTCGTTTCGGGGCAGGGCCTGGAGGGGTGTGGCGTCCATGGCTAAGAAGAGCGTTGGGGATTTGAGTGCCGCTGAGTTGAAGGGGAAGAAGGTGTTTGTGAGGGCTGATTTGAATGTTCCGTTGGATGAGAATCAGAACATTACCGATGACACCAGGATCAGGGCTGCTATCCCTACCATCAAGCACTTGACTGCCAATGGAGCTAAAGTTCTTCTGTCCAGCCACTTGGTGAGTCTACTTTTGGGGCTTCTTAATTAGGCTTTTGCTTTTGCTGTGCTTGGAATGTTGATTTGTGGGTTTTGAGCTGTTTGAAGTGATATTCTCTTGATTAGTGAAATTTCTGGATGCCTTCTACCTGTTGAAATTCCTCGGCATAAGAGTTCTTTCTCTCGTGTAGCTAATTCACGAGATGATCCCTCGGATGTACACTACTTTCCCTTTTAGGATCGAGATTCGTATTTGTAATTTACGGGTATTATCTCATAGTAAATGAATGAAAAAGTTTTCGTTTCCTATAAAAAAAATTGAAAAGAAAAAATGAGAATGTTGGAGTTAGCTTACATTCACACTACGACATTTGATTGTCAAAGAAACTTGTAGTAAATTAATTTTCAGGTAATTGATTCCCATAGATTGAACCTATAAACTCTTACTTCTAAAAGTTTGATCATTTGGCCAATCCATGAGGGTGAATTGGAGTCTTGAGTTGTTGATCAGCCATCCATGGAAACGTGAGTACAGGGTTCAAACTCTTCTTTTTATTGATATAGTGTTTGGAGTCTACTGTTTATGCTACAAAACCTTGTGAATATGTTGTTAACTACGCAAACTTTCAGGGACGACCGAAAGGTGTGACTCCAAAATACAGTTTGGCACCTCTTGTGCCTAGGCTTTCTGAACTCCTTGGCATTGAGGTACCTCTTTCTTATTCATGATCTTATAAAGGGGTTTGTTTTTTTGTTCTTTGCTTTCTTCCTCTCCCATTTCTTGTTAATTATGGCTCCGGATGATCTTTTTACACTTCTGTATGTCATATCCAATCAGGTTGTGAAGGCTGATGACTGCATTGGTCCGGAGGTCGAAAAGTTGGTTGCTTCCCTGCCCGAGGGTGGAGTCCTTCTTCTTGAAAATGTGAGATTCTACAAGGAAGAAGAGAAAAACGAGCCTGAGTTTGCAAAGAAGCTCGCTTCTCTAGCTGATCTTTATGTGAATGATGCTTTTGGAACTGCCCATAGAGCTCATGCTTCTACTGAGGGAGTCACCAAGTTTTTGAAGCCATCCGTAGCTGGCTTTCTCTTGCAAAAGGTAGGATTCGTTAATCGACACTCGATCTACCTGTTACCTGAAAAAGTATCCTATATTATTACGCATGATTTAATTTTTTGTTTTCAATGGTCTTCAATCATAACTGGTAACTGGCTATATTCTCCAAACTCCAATTTTTCATCTCAAACAATGACTGGTCCGATTGTTATCTTGTTTTTAGGAACTTGACTATCTAGTTGGGGCAGTGTCAAGCCCGAAGAGACCATTTGCTGCTATTGTTGGTGGTTCAAAGGTCTCATCGAAGATTGGAGTGATCGAGTCGCTTCTAGAAAAGTGTGACATCCTACTCCTCGGTGGTGGAATGATCTTTACATTTTACAAGGCACAGGGACTTTCGGTTGGTTCATCTTTGGTGGAAGAGGATAAGCTAGACCTAGCAACCTCCCTCCTCGCGAAGGCCAAGGAGAAAGGCGTATCCCTTCTATTACCAACCGATGTTGTCATTGCGGACAAGTTTGCCCCCGATGCAAACAGCAAGGTTTGTTTGTAATCTTGGTTCTTAGATCCATTCCTCATTGCAGACTGTTGCATTGCTTAGTTCTTGCATTTGGCAGATTGTCCCAGCATCTGCTATCCCTGATGGATGGATGGGACTAGACATTGGACCGGATTCCGTGAAGACATTCAATGATGCTTTGGACACTACCCAAACTATCATCTGGAATGGACCCATGGGAGTGTTCGAGTTCGACAAGTTTGCTGTTGGAACAGAGGTAAGGAATAACTTCTCCATGCCAATAAAATTGCACTATACAGAGATTCCATTTTTATACTAATATAGCCAGTGAATATTTGTACATGAGATTTAATTAACTTAGACTCAAAACAAGGACATTGCTACTCTTCTAACTCGTGCGTAATTGCAATGCAACACGCTTTCTATTTCTGGATGTGGGGTGTGTATATTTGTTATAATGCCGAAACTCTACCTGAGAATCTCAGATTCTCAGGTGAGATTCTCAGGTGCTTAGTTTGAAGTTCATCAAAGTATGACATGATAAGAGACTTAAAAACCAATGTAGTTCAACGTGTGCACAGTTATGTATAGCATGTGTCTATGTGGTTCACTTCAATCTGAAACATACCCTTTGTTGCAGGCAATCGCTAAGAAACTCGCAGCGCTTAGCGGAAAGGGTGTGACGACAATCATCGGAGGAGGAGATTCAGTTGCAGCTGTGGAGAAGGTAGGAGTTGCAAGCGTCATGAGCCACATTTCGACTGGGGGAGGTGCTAGTTTGGAGCTGTTGGAAGGCAAAGAGCTTCCAGGCGTTCTTGCTCTTGATGAGGCCGCACCTGTCGCCGCCACTTAGGTAATGGATTATTCAATTTATTTTTTTTCCTCCCATCGCAAGTGAAAGCTTACATTGCAATGTGTAGCAGAGAAATATTTGTTGTAGAAGAAGATGAGTATTTGTTGTAAAAGCTATAACTTCAACTGTTGTTTCCCTTTCTGTCTCTGCATTTGAATTCCTCTTTCTTGCTGGGAACAGACTATAGGGATAACAACTGTTTTAAGAGTTTTAATAAATTTGTGCATTAATGTCC

mRNA sequence

TACCAATTCAATCTCTTTCTCCATTCATTCCTCACAACAACAAAAACCCAAATCGCCAAAACAACTTGGGTTCGCCCATAGCTTCATCAATGGCGTCTGCAGCTTCCCCCACCACATTCCATCTTCTTCCCACACCATCCTCTTCCACTTCATCAACCCGCCCTTCCCTTCTTCAATTTCCCCCCACCGCCGCCTCCTCGATCCGCCTCCGCTCCGCCGCCGCAGACCCACTTTTCTCCGCCCACGTGGCTTCCAAGATTCGGTCGTTTCGGGGCAGGGCCTGGAGGGGTGTGGCGTCCATGGCTAAGAAGAGCGTTGGGGATTTGAGTGCCGCTGAGTTGAAGGGGAAGAAGGTGTTTGTGAGGGCTGATTTGAATGTTCCGTTGGATGAGAATCAGAACATTACCGATGACACCAGGATCAGGGCTGCTATCCCTACCATCAAGCACTTGACTGCCAATGGAGCTAAAGTTCTTCTGTCCAGCCACTTGGGACGACCGAAAGGTGTGACTCCAAAATACAGTTTGGCACCTCTTGTGCCTAGGCTTTCTGAACTCCTTGGCATTGAGGTTGTGAAGGCTGATGACTGCATTGGTCCGGAGGTCGAAAAGTTGGTTGCTTCCCTGCCCGAGGGTGGAGTCCTTCTTCTTGAAAATGTGAGATTCTACAAGGAAGAAGAGAAAAACGAGCCTGAGTTTGCAAAGAAGCTCGCTTCTCTAGCTGATCTTTATGTGAATGATGCTTTTGGAACTGCCCATAGAGCTCATGCTTCTACTGAGGGAGTCACCAAGTTTTTGAAGCCATCCGTAGCTGGCTTTCTCTTGCAAAAGGAACTTGACTATCTAGTTGGGGCAGTGTCAAGCCCGAAGAGACCATTTGCTGCTATTGTTGGTGGTTCAAAGGTCTCATCGAAGATTGGAGTGATCGAGTCGCTTCTAGAAAAGTGTGACATCCTACTCCTCGGTGGTGGAATGATCTTTACATTTTACAAGGCACAGGGACTTTCGGTTGGTTCATCTTTGGTGGAAGAGGATAAGCTAGACCTAGCAACCTCCCTCCTCGCGAAGGCCAAGGAGAAAGGCGTATCCCTTCTATTACCAACCGATGTTGTCATTGCGGACAAGTTTGCCCCCGATGCAAACAGCAAGATTGTCCCAGCATCTGCTATCCCTGATGGATGGATGGGACTAGACATTGGACCGGATTCCGTGAAGACATTCAATGATGCTTTGGACACTACCCAAACTATCATCTGGAATGGACCCATGGGAGTGTTCGAGTTCGACAAGTTTGCTGTTGGAACAGAGGCAATCGCTAAGAAACTCGCAGCGCTTAGCGGAAAGGGTGTGACGACAATCATCGGAGGAGGAGATTCAGTTGCAGCTGTGGAGAAGGTAGGAGTTGCAAGCGTCATGAGCCACATTTCGACTGGGGGAGGTGCTAGTTTGGAGCTGTTGGAAGGCAAAGAGCTTCCAGGCGTTCTTGCTCTTGATGAGGCCGCACCTGTCGCCGCCACTTAGGTAATGGATTATTCAATTTATTTTTTTTCCTCCCATCGCAAGTGAAAGCTTACATTGCAATGTGTAGCAGAGAAATATTTGTTGTAGAAGAAGATGAGTATTTGTTGTAAAAGCTATAACTTCAACTGTTGTTTCCCTTTCTGTCTCTGCATTTGAATTCCTCTTTCTTGCTGGGAACAGACTATAGGGATAACAACTGTTTTAAGAGTTTTAATAAATTTGTGCATTAATGTCC

Coding sequence (CDS)

ATGGCGTCTGCAGCTTCCCCCACCACATTCCATCTTCTTCCCACACCATCCTCTTCCACTTCATCAACCCGCCCTTCCCTTCTTCAATTTCCCCCCACCGCCGCCTCCTCGATCCGCCTCCGCTCCGCCGCCGCAGACCCACTTTTCTCCGCCCACGTGGCTTCCAAGATTCGGTCGTTTCGGGGCAGGGCCTGGAGGGGTGTGGCGTCCATGGCTAAGAAGAGCGTTGGGGATTTGAGTGCCGCTGAGTTGAAGGGGAAGAAGGTGTTTGTGAGGGCTGATTTGAATGTTCCGTTGGATGAGAATCAGAACATTACCGATGACACCAGGATCAGGGCTGCTATCCCTACCATCAAGCACTTGACTGCCAATGGAGCTAAAGTTCTTCTGTCCAGCCACTTGGGACGACCGAAAGGTGTGACTCCAAAATACAGTTTGGCACCTCTTGTGCCTAGGCTTTCTGAACTCCTTGGCATTGAGGTTGTGAAGGCTGATGACTGCATTGGTCCGGAGGTCGAAAAGTTGGTTGCTTCCCTGCCCGAGGGTGGAGTCCTTCTTCTTGAAAATGTGAGATTCTACAAGGAAGAAGAGAAAAACGAGCCTGAGTTTGCAAAGAAGCTCGCTTCTCTAGCTGATCTTTATGTGAATGATGCTTTTGGAACTGCCCATAGAGCTCATGCTTCTACTGAGGGAGTCACCAAGTTTTTGAAGCCATCCGTAGCTGGCTTTCTCTTGCAAAAGGAACTTGACTATCTAGTTGGGGCAGTGTCAAGCCCGAAGAGACCATTTGCTGCTATTGTTGGTGGTTCAAAGGTCTCATCGAAGATTGGAGTGATCGAGTCGCTTCTAGAAAAGTGTGACATCCTACTCCTCGGTGGTGGAATGATCTTTACATTTTACAAGGCACAGGGACTTTCGGTTGGTTCATCTTTGGTGGAAGAGGATAAGCTAGACCTAGCAACCTCCCTCCTCGCGAAGGCCAAGGAGAAAGGCGTATCCCTTCTATTACCAACCGATGTTGTCATTGCGGACAAGTTTGCCCCCGATGCAAACAGCAAGATTGTCCCAGCATCTGCTATCCCTGATGGATGGATGGGACTAGACATTGGACCGGATTCCGTGAAGACATTCAATGATGCTTTGGACACTACCCAAACTATCATCTGGAATGGACCCATGGGAGTGTTCGAGTTCGACAAGTTTGCTGTTGGAACAGAGGCAATCGCTAAGAAACTCGCAGCGCTTAGCGGAAAGGGTGTGACGACAATCATCGGAGGAGGAGATTCAGTTGCAGCTGTGGAGAAGGTAGGAGTTGCAAGCGTCATGAGCCACATTTCGACTGGGGGAGGTGCTAGTTTGGAGCTGTTGGAAGGCAAAGAGCTTCCAGGCGTTCTTGCTCTTGATGAGGCCGCACCTGTCGCCGCCACTTAG

Protein sequence

MASAASPTTFHLLPTPSSSTSSTRPSLLQFPPTAASSIRLRSAAADPLFSAHVASKIRSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTRIRAAIPTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGPEVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAKKLAALSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEAAPVAAT
Homology
BLAST of Sed0026888 vs. NCBI nr
Match: KAG6605349.1 (hypothetical protein SDJN03_02666, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 841.6 bits (2173), Expect = 3.2e-240
Identity = 454/484 (93.80%), Postives = 462/484 (95.45%), Query Frame = 0

Query: 1   MASAASPTTFHLLPTPSSSTSSTRPSLLQFPPTAAS--SIRLRS--------AAADPLFS 60
           MASAAS TTFHLLPT SS TSSTR +LLQF PTAAS  SIRLR+        AAADPLFS
Sbjct: 1   MASAASRTTFHLLPTKSSYTSSTRSALLQFSPTAASSNSIRLRAATTQRLGFAAADPLFS 60

Query: 61  AHVASKIRSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTR 120
            HVASKIRSFRG+A RGV SMAKKSVGDL+AA+LKGKKVFVRADLNVPLD+NQNITDDTR
Sbjct: 61  LHVASKIRSFRGKASRGVVSMAKKSVGDLTAADLKGKKVFVRADLNVPLDDNQNITDDTR 120

Query: 121 IRAAIPTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180
           IRAAIPTIK+LT NGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP
Sbjct: 121 IRAAIPTIKYLTDNGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180

Query: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240
           EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKLDLAT+LLAKAK KGVSLLLPTDVVIADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATTLLAKAKAKGVSLLLPTDVVIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420

Query: 421 KLAALSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 475
           KLA LSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA
Sbjct: 421 KLAELSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 480

BLAST of Sed0026888 vs. NCBI nr
Match: XP_022947340.1 (phosphoglycerate kinase, chloroplastic [Cucurbita moschata] >XP_023532679.1 phosphoglycerate kinase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 841.6 bits (2173), Expect = 3.2e-240
Identity = 454/484 (93.80%), Postives = 462/484 (95.45%), Query Frame = 0

Query: 1   MASAASPTTFHLLPTPSSSTSSTRPSLLQFPPTAAS--SIRLRS--------AAADPLFS 60
           MASAAS TTFHLLPT SS TSSTR +LLQF PTAAS  SIRLR+        AAADPLFS
Sbjct: 1   MASAASRTTFHLLPTKSSYTSSTRSALLQFSPTAASSNSIRLRAAATQRLGFAAADPLFS 60

Query: 61  AHVASKIRSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTR 120
            HVASKIRSFRG+A RGV SMAKKSVGDL+AA+LKGKKVFVRADLNVPLD+NQNITDDTR
Sbjct: 61  LHVASKIRSFRGKASRGVVSMAKKSVGDLTAADLKGKKVFVRADLNVPLDDNQNITDDTR 120

Query: 121 IRAAIPTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180
           IRAAIPTIK+LT NGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP
Sbjct: 121 IRAAIPTIKYLTDNGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180

Query: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240
           EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKLDLAT+LLAKAK KGVSLLLPTDVVIADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATTLLAKAKAKGVSLLLPTDVVIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420

Query: 421 KLAALSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 475
           KLA LSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA
Sbjct: 421 KLAELSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 480

BLAST of Sed0026888 vs. NCBI nr
Match: XP_023007162.1 (phosphoglycerate kinase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 838.2 bits (2164), Expect = 3.6e-239
Identity = 452/484 (93.39%), Postives = 460/484 (95.04%), Query Frame = 0

Query: 1   MASAASPTTFHLLPTPSSSTSSTRPSLLQFPPTAA--SSIRLRS--------AAADPLFS 60
           MASAAS TTFHLLPT SS+TSSTR +LLQF PTAA  SSIRLR+        AAADPLFS
Sbjct: 1   MASAASRTTFHLLPTKSSTTSSTRSALLQFSPTAASSSSIRLRAAATQRLGFAAADPLFS 60

Query: 61  AHVASKIRSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTR 120
            HVASKIRSF G+A RGV SMAKKSVGDL+AA+LKGKKVFVRADLNVPLD+NQNITDDTR
Sbjct: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLTAADLKGKKVFVRADLNVPLDDNQNITDDTR 120

Query: 121 IRAAIPTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180
           IRAAIPTIKHL   GAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP
Sbjct: 121 IRAAIPTIKHLIEKGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180

Query: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240
           EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKLDLAT+LLAKAK KGVSLLLPTDVVIADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATTLLAKAKAKGVSLLLPTDVVIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420

Query: 421 KLAALSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 475
           KLA LSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA
Sbjct: 421 KLAELSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 480

BLAST of Sed0026888 vs. NCBI nr
Match: KAG7010445.1 (hypothetical protein SDJN02_27238, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 838.2 bits (2164), Expect = 3.6e-239
Identity = 449/484 (92.77%), Postives = 462/484 (95.45%), Query Frame = 0

Query: 1   MASAASPTTFHLLPTPSSSTSSTRPSLLQFPPTA--ASSIRLRS--------AAADPLFS 60
           MASAASP TFHLLPT SSSTSSTR +LLQF PTA  +SSIRLRS        AA+DPLFS
Sbjct: 48  MASAASPATFHLLPTTSSSTSSTRSALLQFSPTATSSSSIRLRSAPIQRLGFAASDPLFS 107

Query: 61  AHVASKIRSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTR 120
            HVASKIRSFRG+A RGV SMAKKSVGDL+A++LKGKKVFVRADLNVPLDENQNITDDTR
Sbjct: 108 LHVASKIRSFRGKASRGVVSMAKKSVGDLTASDLKGKKVFVRADLNVPLDENQNITDDTR 167

Query: 121 IRAAIPTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180
           IRAAIPTIKHLT  GAKV+LSSHLGRPKGVTPK+SLAPLVPRLSELLGI+VVKADDCIGP
Sbjct: 168 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGP 227

Query: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240
           EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADL+VNDAFGTAHRAHASTE
Sbjct: 228 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 287

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 288 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 347

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKL+LATSLLAKAK KGVSLLLPTDVVIADKFAPDA
Sbjct: 348 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKAKGVSLLLPTDVVIADKFAPDA 407

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE+IAK
Sbjct: 408 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTESIAK 467

Query: 421 KLAALSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 475
           KLA LSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA
Sbjct: 468 KLAELSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 527

BLAST of Sed0026888 vs. NCBI nr
Match: KAG6570594.1 (hypothetical protein SDJN03_29509, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 838.2 bits (2164), Expect = 3.6e-239
Identity = 449/484 (92.77%), Postives = 462/484 (95.45%), Query Frame = 0

Query: 1   MASAASPTTFHLLPTPSSSTSSTRPSLLQFPPTA--ASSIRLRS--------AAADPLFS 60
           MASAASP TFHLLPT SSSTSSTR +LLQF PTA  +SSIRLRS        AA+DPLFS
Sbjct: 1   MASAASPATFHLLPTTSSSTSSTRSALLQFSPTATSSSSIRLRSAPIQRLGFAASDPLFS 60

Query: 61  AHVASKIRSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTR 120
            HVASKIRSFRG+A RGV SMAKKSVGDL+A++LKGKKVFVRADLNVPLDENQNITDDTR
Sbjct: 61  LHVASKIRSFRGKASRGVVSMAKKSVGDLTASDLKGKKVFVRADLNVPLDENQNITDDTR 120

Query: 121 IRAAIPTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180
           IRAAIPTIKHLT  GAKV+LSSHLGRPKGVTPK+SLAPLVPRLSELLGI+VVKADDCIGP
Sbjct: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGP 180

Query: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240
           EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADL+VNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKL+LATSLLAKAK KGVSLLLPTDVVIADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKAKGVSLLLPTDVVIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE+IAK
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTESIAK 420

Query: 421 KLAALSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 475
           KLA LSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA
Sbjct: 421 KLAELSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 480

BLAST of Sed0026888 vs. ExPASy Swiss-Prot
Match: Q42961 (Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 3.5e-221
Identity = 417/480 (86.88%), Postives = 440/480 (91.67%), Query Frame = 0

Query: 1   MASAASPTTFHLLPTPSSSTS--STRPSLLQF-PPTAASSIRLRSAAADPLFSAHVASKI 60
           MASA +  T   +P  SSST+  +  PS  +F   T    +    AAAD LF+ HVA+K+
Sbjct: 1   MASATASHTLCGIPATSSSTTNKAIAPSSARFLAKTPLRRLGFAGAAADSLFTNHVATKL 60

Query: 61  RSFR--GRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTRIRAAI 120
           RS +   +  RGVASMAKKSVGDL+AAELKGKKVFVRADLNVPLD+NQNITDDTRIRAA+
Sbjct: 61  RSLKSSSKPIRGVASMAKKSVGDLTAAELKGKKVFVRADLNVPLDDNQNITDDTRIRAAV 120

Query: 121 PTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGPEVEKL 180
           PTIKHL ANGAKV+LSSHLGRPKGVTPKYSLAPLVPRLSELLGI+VVK +DCIGPEVEKL
Sbjct: 121 PTIKHLMANGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLGIQVVKVEDCIGPEVEKL 180

Query: 181 VASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKF 240
           VASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKF
Sbjct: 181 VASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKF 240

Query: 241 LKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGM 300
           LKPSVAGFLLQKELDYLVGAVS+PKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGM
Sbjct: 241 LKPSVAGFLLQKELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGM 300

Query: 301 IFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDANSKIV 360
           IFTFYKAQGLSVGSSLVEEDKL+LATSLL KAK KGVSLLLP+DVVIADKFAPDANSKIV
Sbjct: 301 IFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPSDVVIADKFAPDANSKIV 360

Query: 361 PASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAKKLAAL 420
           PASAIPDGWMGLDIGPDSVKTFNDALDTT+T+IWNGPMGVFEFDKFAVGTEAIAKKLA L
Sbjct: 361 PASAIPDGWMGLDIGPDSVKTFNDALDTTKTVIWNGPMGVFEFDKFAVGTEAIAKKLADL 420

Query: 421 SGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA-APVA 475
           SGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGK LPGV+ALDEA APVA
Sbjct: 421 SGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKVLPGVIALDEADAPVA 480

BLAST of Sed0026888 vs. ExPASy Swiss-Prot
Match: P50318 (Phosphoglycerate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g56190 PE=1 SV=3)

HSP 1 Score: 754.2 bits (1946), Expect = 8.9e-217
Identity = 402/476 (84.45%), Postives = 430/476 (90.34%), Query Frame = 0

Query: 1   MASAASPTTFHLLPTPSSS--TSSTRPSLLQFPPTAASSIRLR-SAAADPLFSAHVASKI 60
           MAS A+     ++ +   +  T S+R S    P T+ S+ RL  SA  D  FS HVASK+
Sbjct: 1   MASTAATAALSIIKSTGGAAVTRSSRASFGHIPSTSVSARRLGFSAVVDSRFSVHVASKV 60

Query: 61  RSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTRIRAAIPT 120
            S RG+  RGV +MAKKSVGDL++ +LKGKKVFVRADLNVPLD+NQNITDDTRIRAAIPT
Sbjct: 61  HSVRGKGARGVITMAKKSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPT 120

Query: 121 IKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGPEVEKLVA 180
           IK L  NGAKV+LS+HLGRPKGVTPK+SLAPLVPRLSELLGIEVVKADDCIGPEVE LVA
Sbjct: 121 IKFLIENGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVA 180

Query: 181 SLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLK 240
           SLPEGGVLLLENVRFYKEEEKNEP+FAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLK
Sbjct: 181 SLPEGGVLLLENVRFYKEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLK 240

Query: 241 PSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIF 300
           PSVAGFLLQKELDYLVGAVS+PKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIF
Sbjct: 241 PSVAGFLLQKELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIF 300

Query: 301 TFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDANSKIVPA 360
           TFYKAQGLSVGSSLVEEDKL+LAT+LLAKAK +GVSLLLPTDVVIADKFAPDANSKIVPA
Sbjct: 301 TFYKAQGLSVGSSLVEEDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPDANSKIVPA 360

Query: 361 SAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAKKLAALSG 420
           SAIPDGWMGLDIGPDSVKTFN+ALDTTQT+IWNGPMGVFEF+KFA GTEA+A KLA LS 
Sbjct: 361 SAIPDGWMGLDIGPDSVKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSK 420

Query: 421 KGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEAAPV 474
           KGVTTIIGGGDSVAAVEKVGVA VMSHISTGGGASLELLEGK LPGV+ALDEA PV
Sbjct: 421 KGVTTIIGGGDSVAAVEKVGVAGVMSHISTGGGASLELLEGKVLPGVVALDEATPV 476

BLAST of Sed0026888 vs. ExPASy Swiss-Prot
Match: Q9LD57 (Phosphoglycerate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGK1 PE=1 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 2.9e-215
Identity = 402/479 (83.92%), Postives = 429/479 (89.56%), Query Frame = 0

Query: 1   MASAASPTTFHLLPT----PSSSTSSTRPSLLQFPPTAASSIRLRSAAA--DPLFSAHVA 60
           MASAA+ + F LL +     SS+ +  R SLL  P T+ S+  L  +A      FS HVA
Sbjct: 1   MASAAASSAFSLLKSTGAVASSAGTRARASLLPIPSTSVSARPLGFSATLDSRRFSLHVA 60

Query: 61  SKIRSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTRIRAA 120
           SK+ S RG+  RGV SMAKKSVGDL++A+LKGKKVFVRADLNVPLD+NQ ITDDTRIRAA
Sbjct: 61  SKVESVRGKGSRGVVSMAKKSVGDLTSADLKGKKVFVRADLNVPLDDNQTITDDTRIRAA 120

Query: 121 IPTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGPEVEK 180
           IPTIK+L  NGAKV+LS+HLGRPKGVTPK+SLAPLVPRLSELLGIEV KADDCIGPEVE 
Sbjct: 121 IPTIKYLIENGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVES 180

Query: 181 LVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTK 240
           LVASLPEGGVLLLENVRFYKEEEKN+PEFAKKLASLADLYVNDAFGTAHRAHASTEGVTK
Sbjct: 181 LVASLPEGGVLLLENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTK 240

Query: 241 FLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGG 300
           FLKPSVAGFLLQKELDYLVGAVS+PKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGG
Sbjct: 241 FLKPSVAGFLLQKELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGG 300

Query: 301 MIFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDANSKI 360
           MIFTFYKAQGLSVGSSLVEEDKL+LAT LLAKAK KGVSLLLPTDVV+ADKFAPDANSKI
Sbjct: 301 MIFTFYKAQGLSVGSSLVEEDKLELATELLAKAKAKGVSLLLPTDVVVADKFAPDANSKI 360

Query: 361 VPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAKKLAA 420
           VPAS I DGWMGLDIGPDS+KTFN+ALDTTQT+IWNGPMGVFE +KFA GTEAIA KLA 
Sbjct: 361 VPASGIEDGWMGLDIGPDSIKTFNEALDTTQTVIWNGPMGVFEMEKFAAGTEAIANKLAE 420

Query: 421 LSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEAAPV 474
           LS KGVTTIIGGGDSVAAVEKVGVA VMSHISTGGGASLELLEGK LPGV+ALDEA PV
Sbjct: 421 LSEKGVTTIIGGGDSVAAVEKVGVAGVMSHISTGGGASLELLEGKVLPGVIALDEAIPV 479

BLAST of Sed0026888 vs. ExPASy Swiss-Prot
Match: P29409 (Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea OX=3562 PE=2 SV=2)

HSP 1 Score: 737.6 bits (1903), Expect = 8.6e-212
Identity = 381/428 (89.02%), Postives = 407/428 (95.09%), Query Frame = 0

Query: 49  FSAHVASKIRSFRGRAW---RGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNI 108
           FS HV SKI S++ ++    RGVASMAKKSVGDL++A+LKGKKVFVRADLNVPLD++QNI
Sbjct: 4   FSLHVLSKINSYKSQSTKPIRGVASMAKKSVGDLTSADLKGKKVFVRADLNVPLDDSQNI 63

Query: 109 TDDTRIRAAIPTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKAD 168
           TDDTRIRAAIPTIKHL  NGAKV+LSSHLGRPKGVTPK+SLAPLVPRLSELLG++VVKAD
Sbjct: 64  TDDTRIRAAIPTIKHLINNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGLQVVKAD 123

Query: 169 DCIGPEVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRA 228
           DCIGP+VEKLVA LPEGGVLLLENVRFYKEEEKN+PEFAKKLASLADLYVNDAFGTAHRA
Sbjct: 124 DCIGPDVEKLVAELPEGGVLLLENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRA 183

Query: 229 HASTEGVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEK 288
           HASTEGVTKFLKPSVAGFLLQKELDYLVGAVS+PKRPFAAIVGGSKVSSKIGVIESLLEK
Sbjct: 184 HASTEGVTKFLKPSVAGFLLQKELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEK 243

Query: 289 CDILLLGGGMIFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADK 348
           CDILLLGGGMIFTFYKAQG+SVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADK
Sbjct: 244 CDILLLGGGMIFTFYKAQGMSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADK 303

Query: 349 FAPDANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGT 408
           FA DA+SKIVPAS IPDGWMGLDIGPDS+KTF++ALDTTQT+IWNGPMGVFEF+KFA GT
Sbjct: 304 FAADADSKIVPASGIPDGWMGLDIGPDSIKTFSEALDTTQTVIWNGPMGVFEFEKFAAGT 363

Query: 409 EAIAKKLAALSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVL 468
           EAIAKKL  +S KG TTIIGGGDSVAAVEKVGVA  MSHISTGGGASLELLEGK+LPGVL
Sbjct: 364 EAIAKKLEEISKKGATTIIGGGDSVAAVEKVGVAEAMSHISTGGGASLELLEGKQLPGVL 423

Query: 469 ALDEAAPV 474
           AL+EA PV
Sbjct: 424 ALNEADPV 431

BLAST of Sed0026888 vs. ExPASy Swiss-Prot
Match: P12782 (Phosphoglycerate kinase, chloroplastic OS=Triticum aestivum OX=4565 PE=2 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 1.2e-205
Identity = 392/474 (82.70%), Postives = 421/474 (88.82%), Query Frame = 0

Query: 1   MASAASPTTFHLLPTPSSSTSSTRPSLLQFPPTAASSIRLRS----AAADPLFSAHVASK 60
           MAS A+P    L+   ++S S   P  L+    AA     RS    A ADP  + HVAS+
Sbjct: 1   MASTAAPPA-ALVARRAASASVAAP--LRGAGLAAGCQPARSLAFAAGADPRLAVHVASR 60

Query: 61  IR-SFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTRIRAAI 120
            R +   R  R VA+MAKKSVGDL+AA+L+GK+V VRADLNVPLD+NQNITDDTRIRAAI
Sbjct: 61  CRAASAARGTRAVATMAKKSVGDLTAADLEGKRVLVRADLNVPLDDNQNITDDTRIRAAI 120

Query: 121 PTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGPEVEKL 180
           PTIK+L +NGAKV+L+SHLGRPKGVTPK+SLAPLVPRLSELLGIEV KA+D IGPEVEKL
Sbjct: 121 PTIKYLLSNGAKVILTSHLGRPKGVTPKFSLAPLVPRLSELLGIEVKKAEDVIGPEVEKL 180

Query: 181 VASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKF 240
           VA L  G VLLLENVRFYKEEEKN+PEFAKKLASLADL+VNDAFGTAHRAHASTEGVTKF
Sbjct: 181 VADLANGAVLLLENVRFYKEEEKNDPEFAKKLASLADLFVNDAFGTAHRAHASTEGVTKF 240

Query: 241 LKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGM 300
           LKPSVAGFLLQKELDYL GAVS+PKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGM
Sbjct: 241 LKPSVAGFLLQKELDYLDGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGM 300

Query: 301 IFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDANSKIV 360
           IFTFYKAQGLSVGSSLVEEDKL+LATSLLAKAK KGVSLLLP+DV+IADKFAPDANS+ V
Sbjct: 301 IFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKAKGVSLLLPSDVIIADKFAPDANSQTV 360

Query: 361 PASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAKKLAAL 420
           PASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE+IAKKLA L
Sbjct: 361 PASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTESIAKKLAEL 420

Query: 421 SGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDE 470
           S KGVTTIIGGGDSVAAVEKVGVA VMSHISTGGGASLELLEGKELPGV+ALDE
Sbjct: 421 SKKGVTTIIGGGDSVAAVEKVGVADVMSHISTGGGASLELLEGKELPGVVALDE 471

BLAST of Sed0026888 vs. ExPASy TrEMBL
Match: A0A6J1G6L4 (Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111451230 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.6e-240
Identity = 454/484 (93.80%), Postives = 462/484 (95.45%), Query Frame = 0

Query: 1   MASAASPTTFHLLPTPSSSTSSTRPSLLQFPPTAAS--SIRLRS--------AAADPLFS 60
           MASAAS TTFHLLPT SS TSSTR +LLQF PTAAS  SIRLR+        AAADPLFS
Sbjct: 1   MASAASRTTFHLLPTKSSYTSSTRSALLQFSPTAASSNSIRLRAAATQRLGFAAADPLFS 60

Query: 61  AHVASKIRSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTR 120
            HVASKIRSFRG+A RGV SMAKKSVGDL+AA+LKGKKVFVRADLNVPLD+NQNITDDTR
Sbjct: 61  LHVASKIRSFRGKASRGVVSMAKKSVGDLTAADLKGKKVFVRADLNVPLDDNQNITDDTR 120

Query: 121 IRAAIPTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180
           IRAAIPTIK+LT NGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP
Sbjct: 121 IRAAIPTIKYLTDNGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180

Query: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240
           EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKLDLAT+LLAKAK KGVSLLLPTDVVIADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATTLLAKAKAKGVSLLLPTDVVIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420

Query: 421 KLAALSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 475
           KLA LSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA
Sbjct: 421 KLAELSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 480

BLAST of Sed0026888 vs. ExPASy TrEMBL
Match: A0A6J1KXW8 (Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499738 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 1.7e-239
Identity = 452/484 (93.39%), Postives = 460/484 (95.04%), Query Frame = 0

Query: 1   MASAASPTTFHLLPTPSSSTSSTRPSLLQFPPTAA--SSIRLRS--------AAADPLFS 60
           MASAAS TTFHLLPT SS+TSSTR +LLQF PTAA  SSIRLR+        AAADPLFS
Sbjct: 1   MASAASRTTFHLLPTKSSTTSSTRSALLQFSPTAASSSSIRLRAAATQRLGFAAADPLFS 60

Query: 61  AHVASKIRSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTR 120
            HVASKIRSF G+A RGV SMAKKSVGDL+AA+LKGKKVFVRADLNVPLD+NQNITDDTR
Sbjct: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLTAADLKGKKVFVRADLNVPLDDNQNITDDTR 120

Query: 121 IRAAIPTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180
           IRAAIPTIKHL   GAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP
Sbjct: 121 IRAAIPTIKHLIEKGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180

Query: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240
           EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKLDLAT+LLAKAK KGVSLLLPTDVVIADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATTLLAKAKAKGVSLLLPTDVVIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420

Query: 421 KLAALSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 475
           KLA LSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA
Sbjct: 421 KLAELSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 480

BLAST of Sed0026888 vs. ExPASy TrEMBL
Match: A0A6J1FXV5 (Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111448240 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 1.7e-239
Identity = 449/484 (92.77%), Postives = 462/484 (95.45%), Query Frame = 0

Query: 1   MASAASPTTFHLLPTPSSSTSSTRPSLLQFPPTA--ASSIRLRSA--------AADPLFS 60
           MASAASP TFHLLPT SSSTSSTR +LLQF PTA  +SSIRLRSA        A+DPLFS
Sbjct: 1   MASAASPATFHLLPTTSSSTSSTRSALLQFSPTATSSSSIRLRSAPIQRLGFSASDPLFS 60

Query: 61  AHVASKIRSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTR 120
            HVASKIRSFRG+A RGV SMAKKSVGDL+A++LKGKKVFVRADLNVPLDENQNITDDTR
Sbjct: 61  LHVASKIRSFRGKASRGVVSMAKKSVGDLTASDLKGKKVFVRADLNVPLDENQNITDDTR 120

Query: 121 IRAAIPTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180
           IRAAIPTIKHLT  GAKV+LSSHLGRPKGVTPK+SLAPLVPRLSELLGI+VVKADDCIGP
Sbjct: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGP 180

Query: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240
           EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADL+VNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKL+LATSLLAKAK KGVSLLLPTDVVIADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKAKGVSLLLPTDVVIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE+IAK
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTESIAK 420

Query: 421 KLAALSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 475
           KLA LSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA
Sbjct: 421 KLAELSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 480

BLAST of Sed0026888 vs. ExPASy TrEMBL
Match: A0A6J1JFE9 (Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111483926 PE=3 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.3e-239
Identity = 448/484 (92.56%), Postives = 462/484 (95.45%), Query Frame = 0

Query: 1   MASAASPTTFHLLPTPSSSTSSTRPSLLQFPPTA--ASSIRLRS--------AAADPLFS 60
           MASAASP TFHLLPT SSSTSSTR +LLQF PTA  +SSIRLRS        AA+DPLFS
Sbjct: 1   MASAASPATFHLLPTTSSSTSSTRSALLQFSPTATSSSSIRLRSAPIQRLGFAASDPLFS 60

Query: 61  AHVASKIRSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTR 120
            HVASKIRSFRG+A RGV SMAKKSVGDL+A++LKGKKVFVRADLNVPLDENQNITDDTR
Sbjct: 61  LHVASKIRSFRGKASRGVVSMAKKSVGDLTASDLKGKKVFVRADLNVPLDENQNITDDTR 120

Query: 121 IRAAIPTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180
           IRAAIPTIKHLT  GAKV+LSSHLGRPKGVTPK+SLAPLVPRLSELLGI+VVKADDCIGP
Sbjct: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGP 180

Query: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240
           EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADL+VNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKL+LATSLLAKAK KGVSLLLPTDVVIADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKAKGVSLLLPTDVVIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE+IAK
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTESIAK 420

Query: 421 KLAALSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 475
           KLA LSGKGVTTIIGGGDSVAAVEKVGVAS+MSHISTGGGASLELLEGKELPGVLALDEA
Sbjct: 421 KLAELSGKGVTTIIGGGDSVAAVEKVGVASIMSHISTGGGASLELLEGKELPGVLALDEA 480

BLAST of Sed0026888 vs. ExPASy TrEMBL
Match: A0A5D3CBI0 (Phosphoglycerate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3734G00020 PE=3 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 2.8e-237
Identity = 446/484 (92.15%), Postives = 458/484 (94.63%), Query Frame = 0

Query: 1   MASAASPTTFHLLPTPSSSTSSTRPSLLQFPP--TAASSIRLRS--------AAADPLFS 60
           MASAAS  TFHLLPT SSSTSSTR +LLQF P  T++SSIRLRS        AAADPLFS
Sbjct: 1   MASAASRATFHLLPTTSSSTSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60

Query: 61  AHVASKIRSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTR 120
            HVASKIRSF G+A RGV SMAKKSVGDL+AA+LKGKKVFVRADLNVPLD+NQNITDDTR
Sbjct: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLTAADLKGKKVFVRADLNVPLDDNQNITDDTR 120

Query: 121 IRAAIPTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGP 180
           IRAAIPTIKHLT  GAKV+LSSHLGRPKGVTPKYSLAPLVPRLSELLG +VVKADDCIGP
Sbjct: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLGFQVVKADDCIGP 180

Query: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240
           EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADL+VNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKL+LATSLL KAK KGVSLLLPTDV+IADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAK 420

Query: 421 KLAALSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 475
           KLA LSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA
Sbjct: 421 KLAELSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 480

BLAST of Sed0026888 vs. TAIR 10
Match: AT1G56190.1 (Phosphoglycerate kinase family protein )

HSP 1 Score: 754.2 bits (1946), Expect = 6.3e-218
Identity = 402/476 (84.45%), Postives = 430/476 (90.34%), Query Frame = 0

Query: 1   MASAASPTTFHLLPTPSSS--TSSTRPSLLQFPPTAASSIRLR-SAAADPLFSAHVASKI 60
           MAS A+     ++ +   +  T S+R S    P T+ S+ RL  SA  D  FS HVASK+
Sbjct: 1   MASTAATAALSIIKSTGGAAVTRSSRASFGHIPSTSVSARRLGFSAVVDSRFSVHVASKV 60

Query: 61  RSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTRIRAAIPT 120
            S RG+  RGV +MAKKSVGDL++ +LKGKKVFVRADLNVPLD+NQNITDDTRIRAAIPT
Sbjct: 61  HSVRGKGARGVITMAKKSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPT 120

Query: 121 IKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGPEVEKLVA 180
           IK L  NGAKV+LS+HLGRPKGVTPK+SLAPLVPRLSELLGIEVVKADDCIGPEVE LVA
Sbjct: 121 IKFLIENGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVA 180

Query: 181 SLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLK 240
           SLPEGGVLLLENVRFYKEEEKNEP+FAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLK
Sbjct: 181 SLPEGGVLLLENVRFYKEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLK 240

Query: 241 PSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIF 300
           PSVAGFLLQKELDYLVGAVS+PKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIF
Sbjct: 241 PSVAGFLLQKELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIF 300

Query: 301 TFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDANSKIVPA 360
           TFYKAQGLSVGSSLVEEDKL+LAT+LLAKAK +GVSLLLPTDVVIADKFAPDANSKIVPA
Sbjct: 301 TFYKAQGLSVGSSLVEEDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPDANSKIVPA 360

Query: 361 SAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAKKLAALSG 420
           SAIPDGWMGLDIGPDSVKTFN+ALDTTQT+IWNGPMGVFEF+KFA GTEA+A KLA LS 
Sbjct: 361 SAIPDGWMGLDIGPDSVKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSK 420

Query: 421 KGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEAAPV 474
           KGVTTIIGGGDSVAAVEKVGVA VMSHISTGGGASLELLEGK LPGV+ALDEA PV
Sbjct: 421 KGVTTIIGGGDSVAAVEKVGVAGVMSHISTGGGASLELLEGKVLPGVVALDEATPV 476

BLAST of Sed0026888 vs. TAIR 10
Match: AT3G12780.1 (phosphoglycerate kinase 1 )

HSP 1 Score: 749.2 bits (1933), Expect = 2.0e-216
Identity = 402/479 (83.92%), Postives = 429/479 (89.56%), Query Frame = 0

Query: 1   MASAASPTTFHLLPT----PSSSTSSTRPSLLQFPPTAASSIRLRSAAA--DPLFSAHVA 60
           MASAA+ + F LL +     SS+ +  R SLL  P T+ S+  L  +A      FS HVA
Sbjct: 1   MASAAASSAFSLLKSTGAVASSAGTRARASLLPIPSTSVSARPLGFSATLDSRRFSLHVA 60

Query: 61  SKIRSFRGRAWRGVASMAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTRIRAA 120
           SK+ S RG+  RGV SMAKKSVGDL++A+LKGKKVFVRADLNVPLD+NQ ITDDTRIRAA
Sbjct: 61  SKVESVRGKGSRGVVSMAKKSVGDLTSADLKGKKVFVRADLNVPLDDNQTITDDTRIRAA 120

Query: 121 IPTIKHLTANGAKVLLSSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGPEVEK 180
           IPTIK+L  NGAKV+LS+HLGRPKGVTPK+SLAPLVPRLSELLGIEV KADDCIGPEVE 
Sbjct: 121 IPTIKYLIENGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVES 180

Query: 181 LVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTK 240
           LVASLPEGGVLLLENVRFYKEEEKN+PEFAKKLASLADLYVNDAFGTAHRAHASTEGVTK
Sbjct: 181 LVASLPEGGVLLLENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTK 240

Query: 241 FLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGG 300
           FLKPSVAGFLLQKELDYLVGAVS+PKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGG
Sbjct: 241 FLKPSVAGFLLQKELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGG 300

Query: 301 MIFTFYKAQGLSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDANSKI 360
           MIFTFYKAQGLSVGSSLVEEDKL+LAT LLAKAK KGVSLLLPTDVV+ADKFAPDANSKI
Sbjct: 301 MIFTFYKAQGLSVGSSLVEEDKLELATELLAKAKAKGVSLLLPTDVVVADKFAPDANSKI 360

Query: 361 VPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAKKLAA 420
           VPAS I DGWMGLDIGPDS+KTFN+ALDTTQT+IWNGPMGVFE +KFA GTEAIA KLA 
Sbjct: 361 VPASGIEDGWMGLDIGPDSIKTFNEALDTTQTVIWNGPMGVFEMEKFAAGTEAIANKLAE 420

Query: 421 LSGKGVTTIIGGGDSVAAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEAAPV 474
           LS KGVTTIIGGGDSVAAVEKVGVA VMSHISTGGGASLELLEGK LPGV+ALDEA PV
Sbjct: 421 LSEKGVTTIIGGGDSVAAVEKVGVAGVMSHISTGGGASLELLEGKVLPGVIALDEAIPV 479

BLAST of Sed0026888 vs. TAIR 10
Match: AT1G56190.2 (Phosphoglycerate kinase family protein )

HSP 1 Score: 724.2 bits (1868), Expect = 7.0e-209
Identity = 373/403 (92.56%), Postives = 390/403 (96.77%), Query Frame = 0

Query: 71  MAKKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTRIRAAIPTIKHLTANGAKVLL 130
           MAKKSVGDL++ +LKGKKVFVRADLNVPLD+NQNITDDTRIRAAIPTIK L  NGAKV+L
Sbjct: 1   MAKKSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAKVIL 60

Query: 131 SSHLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGPEVEKLVASLPEGGVLLLENV 190
           S+HLGRPKGVTPK+SLAPLVPRLSELLGIEVVKADDCIGPEVE LVASLPEGGVLLLENV
Sbjct: 61  STHLGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENV 120

Query: 191 RFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELD 250
           RFYKEEEKNEP+FAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELD
Sbjct: 121 RFYKEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELD 180

Query: 251 YLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSS 310
           YLVGAVS+PKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSS
Sbjct: 181 YLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSS 240

Query: 311 LVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIG 370
           LVEEDKL+LAT+LLAKAK +GVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIG
Sbjct: 241 LVEEDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIG 300

Query: 371 PDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAKKLAALSGKGVTTIIGGGDSV 430
           PDSVKTFN+ALDTTQT+IWNGPMGVFEF+KFA GTEA+A KLA LS KGVTTIIGGGDSV
Sbjct: 301 PDSVKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSKKGVTTIIGGGDSV 360

Query: 431 AAVEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEAAPV 474
           AAVEKVGVA VMSHISTGGGASLELLEGK LPGV+ALDEA PV
Sbjct: 361 AAVEKVGVAGVMSHISTGGGASLELLEGKVLPGVVALDEATPV 403

BLAST of Sed0026888 vs. TAIR 10
Match: AT1G79550.1 (phosphoglycerate kinase )

HSP 1 Score: 672.9 bits (1735), Expect = 1.8e-193
Identity = 343/398 (86.18%), Postives = 371/398 (93.22%), Query Frame = 0

Query: 73  KKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTRIRAAIPTIKHLTANGAKVLLSS 132
           K+SVG L  A+LKGK VFVR DLNVPLD+N NITDDTRIRAA+PTIK+L  NG++V+L S
Sbjct: 4   KRSVGTLKEADLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSRVVLCS 63

Query: 133 HLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGPEVEKLVASLPEGGVLLLENVRF 192
           HLGRPKGVTPKYSL PLVPRLSELLG+EVV A+D IG EV+KLVA LPEGGVLLLENVRF
Sbjct: 64  HLGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLENVRF 123

Query: 193 YKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYL 252
           Y EEEKN+PEFAKKLA+LAD+YVNDAFGTAHRAHASTEGV KFLKPSVAGFL+QKELDYL
Sbjct: 124 YAEEEKNDPEFAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKELDYL 183

Query: 253 VGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLV 312
           VGAV++PK+PFAAIVGGSKVS+KIGVIESLL   DILLLGGGMIFTFYKAQGLSVGSSLV
Sbjct: 184 VGAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGMIFTFYKAQGLSVGSSLV 243

Query: 313 EEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPD 372
           EEDKLDLA SL+ KAK KGVSLLLPTDVVIADKFAPDANSKIVPA+AIPDGWMGLDIGPD
Sbjct: 244 EEDKLDLAKSLMEKAKAKGVSLLLPTDVVIADKFAPDANSKIVPATAIPDGWMGLDIGPD 303

Query: 373 SVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAKKLAALSGKGVTTIIGGGDSVAA 432
           S+KTF++ALDTT+TIIWNGPMGVFEFDKFA GTEA+AK+LA LSGKGVTTIIGGGDSVAA
Sbjct: 304 SIKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGVTTIIGGGDSVAA 363

Query: 433 VEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 471
           VEKVG+A  MSHISTGGGASLELLEGK LPGVLALDEA
Sbjct: 364 VEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

BLAST of Sed0026888 vs. TAIR 10
Match: AT1G79550.2 (phosphoglycerate kinase )

HSP 1 Score: 672.9 bits (1735), Expect = 1.8e-193
Identity = 343/398 (86.18%), Postives = 371/398 (93.22%), Query Frame = 0

Query: 73  KKSVGDLSAAELKGKKVFVRADLNVPLDENQNITDDTRIRAAIPTIKHLTANGAKVLLSS 132
           K+SVG L  A+LKGK VFVR DLNVPLD+N NITDDTRIRAA+PTIK+L  NG++V+L S
Sbjct: 4   KRSVGTLKEADLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSRVVLCS 63

Query: 133 HLGRPKGVTPKYSLAPLVPRLSELLGIEVVKADDCIGPEVEKLVASLPEGGVLLLENVRF 192
           HLGRPKGVTPKYSL PLVPRLSELLG+EVV A+D IG EV+KLVA LPEGGVLLLENVRF
Sbjct: 64  HLGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLENVRF 123

Query: 193 YKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYL 252
           Y EEEKN+PEFAKKLA+LAD+YVNDAFGTAHRAHASTEGV KFLKPSVAGFL+QKELDYL
Sbjct: 124 YAEEEKNDPEFAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKELDYL 183

Query: 253 VGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLV 312
           VGAV++PK+PFAAIVGGSKVS+KIGVIESLL   DILLLGGGMIFTFYKAQGLSVGSSLV
Sbjct: 184 VGAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGMIFTFYKAQGLSVGSSLV 243

Query: 313 EEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPD 372
           EEDKLDLA SL+ KAK KGVSLLLPTDVVIADKFAPDANSKIVPA+AIPDGWMGLDIGPD
Sbjct: 244 EEDKLDLAKSLMEKAKAKGVSLLLPTDVVIADKFAPDANSKIVPATAIPDGWMGLDIGPD 303

Query: 373 SVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAKKLAALSGKGVTTIIGGGDSVAA 432
           S+KTF++ALDTT+TIIWNGPMGVFEFDKFA GTEA+AK+LA LSGKGVTTIIGGGDSVAA
Sbjct: 304 SIKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGVTTIIGGGDSVAA 363

Query: 433 VEKVGVASVMSHISTGGGASLELLEGKELPGVLALDEA 471
           VEKVG+A  MSHISTGGGASLELLEGK LPGVLALDEA
Sbjct: 364 VEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6605349.13.2e-24093.80hypothetical protein SDJN03_02666, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022947340.13.2e-24093.80phosphoglycerate kinase, chloroplastic [Cucurbita moschata] >XP_023532679.1 phos... [more]
XP_023007162.13.6e-23993.39phosphoglycerate kinase, chloroplastic [Cucurbita maxima][more]
KAG7010445.13.6e-23992.77hypothetical protein SDJN02_27238, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6570594.13.6e-23992.77hypothetical protein SDJN03_29509, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q429613.5e-22186.88Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
P503188.9e-21784.45Phosphoglycerate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
Q9LD572.9e-21583.92Phosphoglycerate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGK1... [more]
P294098.6e-21289.02Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea OX=3562 P... [more]
P127821.2e-20582.70Phosphoglycerate kinase, chloroplastic OS=Triticum aestivum OX=4565 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G6L41.6e-24093.80Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111451230 PE=3 SV=1[more]
A0A6J1KXW81.7e-23993.39Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499738 PE=3 SV=1[more]
A0A6J1FXV51.7e-23992.77Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111448240 PE=3 SV=1[more]
A0A6J1JFE92.3e-23992.56Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111483926 PE=3 SV=1[more]
A0A5D3CBI02.8e-23792.15Phosphoglycerate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
AT1G56190.16.3e-21884.45Phosphoglycerate kinase family protein [more]
AT3G12780.12.0e-21683.92phosphoglycerate kinase 1 [more]
AT1G56190.27.0e-20992.56Phosphoglycerate kinase family protein [more]
AT1G79550.11.8e-19386.18phosphoglycerate kinase [more]
AT1G79550.21.8e-19386.18phosphoglycerate kinase [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001576Phosphoglycerate kinasePRINTSPR00477PHGLYCKINASEcoord: 422..433
score: 62.91
coord: 241..263
score: 52.13
coord: 182..197
score: 70.19
coord: 210..232
score: 70.95
coord: 445..462
score: 85.96
coord: 386..411
score: 60.99
coord: 264..283
score: 60.33
coord: 105..127
score: 55.14
coord: 83..99
score: 60.05
IPR001576Phosphoglycerate kinasePIRSFPIRSF000724Pgkcoord: 69..475
e-value: 1.6E-152
score: 505.8
IPR001576Phosphoglycerate kinasePFAMPF00162PGKcoord: 81..459
e-value: 1.9E-160
score: 534.0
IPR001576Phosphoglycerate kinasePANTHERPTHR11406PHOSPHOGLYCERATE KINASEcoord: 1..470
IPR001576Phosphoglycerate kinaseHAMAPMF_00145Phosphoglyc_kinasecoord: 78..469
score: 41.791485
IPR001576Phosphoglycerate kinaseCDDcd00318Phosphoglycerate_kinasecoord: 84..469
e-value: 0.0
score: 618.885
IPR015824Phosphoglycerate kinase, N-terminalGENE3D3.40.50.1260coord: 144..471
e-value: 5.2E-141
score: 472.0
IPR015824Phosphoglycerate kinase, N-terminalGENE3D3.40.50.1260coord: 50..142
e-value: 1.7E-23
score: 85.3
IPR015824Phosphoglycerate kinase, N-terminalGENE3D3.40.50.1260coord: 258..445
e-value: 5.2E-141
score: 472.0
NoneNo IPR availablePIRSRPIRSR000724-2PIRSR000724-2coord: 79..310
e-value: 1.2E-54
score: 183.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availablePANTHERPTHR11406:SF29PHOSPHOGLYCERATE KINASEcoord: 1..470
IPR015911Phosphoglycerate kinase, conserved sitePROSITEPS00111PGLYCERATE_KINASEcoord: 88..98
IPR036043Phosphoglycerate kinase superfamilySUPERFAMILY53748Phosphoglycerate kinasecoord: 78..469

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0026888.1Sed0026888.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006096 glycolytic process
cellular_component GO:0005829 cytosol
molecular_function GO:0043531 ADP binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0004618 phosphoglycerate kinase activity