Sed0026596 (gene) Chayote v1

BLAST of Sed0026596 vs. NCBI nr
Match: XP_038889084.1 (DNA ligase 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 1969.1 bits (5100), Expect = 0.0e+00
Identity = 1023/1235 (82.83%), Postives = 1105/1235 (89.47%), Query Frame = 0

Query: 1    MTESMKFSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSPSDYFSAIRLILPSLDRERRT 60
            M+E +KF VLSSLFDWIQRSKSS KKRSKFRKFLDTFC+P+DYFSAIRLILP+LDRER T
Sbjct: 1    MSEPIKFGVLSSLFDWIQRSKSSAKKRSKFRKFLDTFCTPADYFSAIRLILPNLDRERGT 60

Query: 61   YGLKESVLATSLIDALGMSRDSDDALRLINWRKAGARAGANAGNFALLAAEVLQRRQGMT 120
            YGLKESVLAT LIDALGMSR+SDDA RLINWRK GA+ GANAGNFAL+AAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGMSRESDDARRLINWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIKELNDLLDRLALSENRAEKTVVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180
            PGGLTIKELNDLLDRLA  ENRAEKT VLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS
Sbjct: 121  PGGLTIKELNDLLDRLASGENRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180

Query: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240
            IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK
Sbjct: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240

Query: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLSTRCILD 300
            KLHGKEVV ECKFDGDRIQIHKNGTE+HYFSRNFLDHSEYGHGMSDV+V NVLSTRCILD
Sbjct: 241  KLHGKEVVAECKFDGDRIQIHKNGTEVHYFSRNFLDHSEYGHGMSDVVVHNVLSTRCILD 300

Query: 301  GEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDVLYVGDTSLIHRSLKERR 360
            GEMLVWDKSLNRFAEFGSNQEIA+AAKDGFDSNRQLCYVAFDVLYVGDTS+IHRSLKER 
Sbjct: 301  GEMLVWDKSLNRFAEFGSNQEIARAAKDGFDSNRQLCYVAFDVLYVGDTSVIHRSLKERH 360

Query: 361  ELLRNVVKPVKGRLEFLVPNGGLNNDCAGDPSWSMRAYNVDDVERFFKNTIENRDEGIVI 420
            ELLR+VVKPVKG+LE LVPNGGLN+DCAG PSWS+RAYNVDDVE+FFK+TIENRDEGIVI
Sbjct: 361  ELLRDVVKPVKGQLEILVPNGGLNSDCAGYPSWSIRAYNVDDVEKFFKSTIENRDEGIVI 420

Query: 421  KDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLADRPH 480
            KDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLA+RP 
Sbjct: 421  KDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERPP 480

Query: 481  SNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKKTAPSFYQVTNNSKERPDV 540
            SN +PRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYP+KTAPSFYQVTNNSKERPDV
Sbjct: 481  SNGYPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPRKTAPSFYQVTNNSKERPDV 540

Query: 541  WIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHS 600
            WIESPEKSIILSITSDIRTI SEVFAAPYSLRFPRIDRVR DKPWHECLDVQSFVELVHS
Sbjct: 541  WIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRLDKPWHECLDVQSFVELVHS 600

Query: 601  SNGTTQ-------GQDSKQKYIKSTGKGRKKNISAVPSHLLQTDISGIKEDSLIFSNMLF 660
            SNGTTQ        QDSK+KYIKSTGKGRKK++S VPSHLLQTDI+GI EDSLIFSNMLF
Sbjct: 601  SNGTTQRGTNNSGWQDSKRKYIKSTGKGRKKSVSIVPSHLLQTDITGITEDSLIFSNMLF 660

Query: 661  YIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNIAADSKGIKYEAAKRHGDVIHYSW 720
            YIVNVPP++SLDSLHKLIVENGGTFSMNLN+SV H+IAADSKGIKYEAAKRHGDVIHYSW
Sbjct: 661  YIVNVPPSHSLDSLHKLIVENGGTFSMNLNNSVTHSIAADSKGIKYEAAKRHGDVIHYSW 720

Query: 721  VLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLLNNVSRSQ 780
            VLECCSQKKLLPL+PK+FLFLS HSKKKLEEEIDEFSDSYFWDLEL+DLKQLL+NVSRS+
Sbjct: 721  VLECCSQKKLLPLKPKHFLFLSGHSKKKLEEEIDEFSDSYFWDLELSDLKQLLSNVSRSE 780

Query: 781  DVKAIDYYRGKYCPKHEWCIFVGCQIYFLPLRPSLKSDWDVLFELLIRRLKVEVSFRGGK 840
            DVKAIDYYRGKYCPK EWC+FVGCQIYFLPLR  LKSDW VL EL +RRLKVEVSFRGGK
Sbjct: 781  DVKAIDYYRGKYCPKREWCMFVGCQIYFLPLRLYLKSDWGVLLELSVRRLKVEVSFRGGK 840

Query: 841  VSDNHVSATHIVVFSMPGSPMGYETVLKRCSEAEKHVLLRDKIHIVAHQWLEDCLEMAHR 900
            VSD+HVSATHIVVFS+PG+P+ YETVLK C EAEKH +L+DKIHIV HQWLEDCLE A R
Sbjct: 841  VSDDHVSATHIVVFSIPGAPVRYETVLKSCDEAEKHAMLKDKIHIVTHQWLEDCLERAQR 900

Query: 901  LQEDAYSLNPNREKSIEKSNSGVDAAILPASENIENQQTSSPSKEYKNRESNKAASGKHM 960
            LQEDAYSLNPN +KSI+  NSG+D  +  ASENI+NQQ  SPS+EYK++  N+AASG+H 
Sbjct: 901  LQEDAYSLNPNGKKSIDNINSGMDFEMPSASENIDNQQIPSPSQEYKDQGRNEAASGQHT 960

Query: 961  LSYSTETTGEKKRGRPAARSLQKRTSGVEQVRRARARTTKGPAKISEVDSENSDHSDEKI 1020
            LSYSTE +G KKR RPAARS+QK  SGV+QVR  R RT KGPAKISEV+S+NSD++DEK 
Sbjct: 961  LSYSTERSGGKKRRRPAARSMQKGKSGVKQVREFRTRTAKGPAKISEVESDNSDYTDEKT 1020

Query: 1021 G----NITTVRCENSTMNEFQTLENERMDEKKRGRP-ARNAPKRKAGVEPVRRTRARTAK 1080
                 N+ T+  E S  NEF+TLENE  + KKRGRP A N  K KAG + VRRTRAR  K
Sbjct: 1021 NAETRNVGTLCSETSEKNEFKTLENEISNTKKRGRPTAGNTQKGKAGAKAVRRTRARITK 1080

Query: 1081 RSASISNIFSDNSDSTNETGMEKGNIGTL---WEENSKTDEFKTL-EHDSKQKGKAIEQN 1140
             SA I +I S ++DST+    EK N   L    EENSK DEFK L +HD KQKGKAIE+N
Sbjct: 1081 GSAKIGDIESGSNDSTD----EKTNTDELRKEKEENSKRDEFKMLIDHDPKQKGKAIEEN 1140

Query: 1141 DLAEPKSSAH-----EVDISERYKYK-VEKSEKLEMMTDPLQAMLLDMVPSLGMSETKSM 1200
              A+  S+       EV ISERY +K VEK EKLE+M DPL+AMLLDMVPSLGMS +KS 
Sbjct: 1141 VRADFGSNVDFGKVPEVGISERYDFKDVEKCEKLEIMKDPLEAMLLDMVPSLGMSGSKSS 1200

Query: 1201 STVLEEKQPIHNNSQPAAKKKVSYKDVASELLKDW 1214
            STV+EEK+P+++NS+P AKKKVSYKDVASELLKDW
Sbjct: 1201 STVVEEKKPVNSNSEP-AKKKVSYKDVASELLKDW 1230

BLAST of Sed0026596 vs. NCBI nr
Match: XP_022996609.1 (DNA ligase 4 [Cucurbita maxima])

HSP 1 Score: 1963.3 bits (5085), Expect = 0.0e+00
Identity = 1014/1228 (82.57%), Postives = 1094/1228 (89.09%), Query Frame = 0

Query: 1    MTESMKFSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSPSDYFSAIRLILPSLDRERRT 60
            MTE +KFSVLSSLFDWIQRSKSS KKRSKFRKF+DTFCSP+DYFSAIRLILPSLDRER T
Sbjct: 1    MTELIKFSVLSSLFDWIQRSKSSAKKRSKFRKFIDTFCSPADYFSAIRLILPSLDRERGT 60

Query: 61   YGLKESVLATSLIDALGMSRDSDDALRLINWRKAGARAGANAGNFALLAAEVLQRRQGMT 120
            YGLKESVLAT LIDALGMSR+S+DALRLINWRK GA+ GAN GNFAL+AAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGMSRESEDALRLINWRKGGAKTGANVGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIKELNDLLDRLALSENRAEKTVVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180
            PGGL IKELNDLLDRLALSENRAEKT VLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS
Sbjct: 121  PGGLLIKELNDLLDRLALSENRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180

Query: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240
            IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK
Sbjct: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240

Query: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLSTRCILD 300
            KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVL+TRCILD
Sbjct: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLATRCILD 300

Query: 301  GEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDVLYVGDTSLIHRSLKERR 360
            GEMLVWDKSLNRFAEFGSNQEIA+AAKDGFDSNRQLCYVAFDVLYVGDTS+IHRSLKER 
Sbjct: 301  GEMLVWDKSLNRFAEFGSNQEIARAAKDGFDSNRQLCYVAFDVLYVGDTSVIHRSLKERH 360

Query: 361  ELLRNVVKPVKGRLEFLVPNGGLNNDCAGDPSWSMRAYNVDDVERFFKNTIENRDEGIVI 420
            ELLR+VVKPVKG+LE LVPNGGLN+DCAGDP WS+RAYNVDDVERFFK+TIENRDEGIVI
Sbjct: 361  ELLRDVVKPVKGQLEILVPNGGLNSDCAGDPPWSIRAYNVDDVERFFKSTIENRDEGIVI 420

Query: 421  KDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLADRPH 480
            KDL SKWEPGDRSGKWLKLKPDY+ AGSDLDALIIGGYYGSGRRGGEVAQFLMGLA+ P 
Sbjct: 421  KDLSSKWEPGDRSGKWLKLKPDYIHAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAEHPP 480

Query: 481  SNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKKTAPSFYQVTNNSKERPDV 540
            SNA+PRRFISFCRVGTGLTDEELD+VVNKLKPYFRKSEYP+KTAPSFYQVTNNSKERPDV
Sbjct: 481  SNAYPRRFISFCRVGTGLTDEELDSVVNKLKPYFRKSEYPRKTAPSFYQVTNNSKERPDV 540

Query: 541  WIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHS 600
            WIESPEKSIILSITSDIRTI SEVFAAPYSLRFPRI RVRYDKPWHECLDVQSFVELVHS
Sbjct: 541  WIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIHRVRYDKPWHECLDVQSFVELVHS 600

Query: 601  SNGTTQ-------GQDSKQKYIKSTGKGRKKNISAVPSHLLQTDISGIKEDSLIFSNMLF 660
            SNGTTQ        QDSK+K I STGKGRKK++S VPSHLLQTDISGIK DSLIFSNMLF
Sbjct: 601  SNGTTQRGTNNSGWQDSKRKDINSTGKGRKKSVSVVPSHLLQTDISGIKGDSLIFSNMLF 660

Query: 661  YIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNIAADSKGIKYEAAKRHGDVIHYSW 720
            YIVNVPPT+SLDSLHKLIVENGGTFSMNLN+SV H++AADSKGIKYEAAKRHGDVIHYSW
Sbjct: 661  YIVNVPPTHSLDSLHKLIVENGGTFSMNLNNSVTHSVAADSKGIKYEAAKRHGDVIHYSW 720

Query: 721  VLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLLNNVSRSQ 780
            VLECCSQKKLLPL PK+FLFLS HSKKKLEEEIDEFSDSYFWDLELADLKQLL+NVSRS+
Sbjct: 721  VLECCSQKKLLPLMPKHFLFLSGHSKKKLEEEIDEFSDSYFWDLELADLKQLLSNVSRSE 780

Query: 781  DVKAIDYYRGKYCPKHEWCIFVGCQIYFLPLRPSLKSDWDVLFELLIRRLKVEVSFRGGK 840
            DVKAIDYYR KYCPKHEWCIF GCQIYF PL+PSLKSDWD L EL +RRLKVEVSFRGGK
Sbjct: 781  DVKAIDYYREKYCPKHEWCIFSGCQIYFHPLKPSLKSDWDSLLELSVRRLKVEVSFRGGK 840

Query: 841  VSDNHVSATHIVVFSMPGSPMGYETVLKRCSEAEKHVLLRDKIHIVAHQWLEDCLEMAHR 900
            VSD+ VSATHIVVF +PG P+  E VLK C+EA+KHV+L+DK HIVAHQWLEDCLE A R
Sbjct: 841  VSDDRVSATHIVVFCIPGPPVKCEAVLKSCNEADKHVMLKDKTHIVAHQWLEDCLERAQR 900

Query: 901  LQEDAYSLNPNREKSIEKSNSGVDAAILPASENIENQQTSSPSKEYKNRESNKAASGKHM 960
            LQEDAYSLNPN +KSIEK NSG+ +    ASENI+NQQ  SPSKEYK+   N++ASG+HM
Sbjct: 901  LQEDAYSLNPNTKKSIEKLNSGIGSETPSASENIDNQQIPSPSKEYKDLGGNESASGQHM 960

Query: 961  LSYSTETTGEKKRGRPAARSLQKRTSGVEQVRRARARTTKGPAKISEVDSENSDHSDEK- 1020
            LS+ST+ +G KKRGRPA  S+QK  S +EQVRR   R TKGPAKI+EV+S++SDH++EK 
Sbjct: 961  LSHSTKRSGGKKRGRPAESSMQKGKSSIEQVRRVFPRRTKGPAKINEVESDSSDHTNEKT 1020

Query: 1021 ---IGNITTVRCENSTMNEFQTLENERMDEKKRGRPARNAPKRKAGVEPVRRTRARTAKR 1080
                GNI T+  E+   NEF+ LENER  +KKRG PARN  K KA VE VRRTRAR AK 
Sbjct: 1021 NAETGNIGTLCSESFGKNEFRMLENERPVKKKRGSPARNTQKGKARVEQVRRTRARIAKG 1080

Query: 1081 SASISNIFSDNSDSTNETGMEKGNIGTLWEENSKTDEFKTLEH-DSKQKGKAIEQNDLAE 1140
              S    ++D   +  E  ME+GN+GT+ ++NSKT+E K LE  D KQKGKAIEQ+D+A+
Sbjct: 1081 DGS---DYTDVKKNAEEAEMERGNVGTVCKDNSKTEELKMLEDCDWKQKGKAIEQSDVAD 1140

Query: 1141 PKSSAHEVDISERYKYKVEKSEKLEMMTDPLQAMLLDMVPSLGMSETK---SMSTVLEEK 1200
            PKS               + SEKLE+MTDPLQAMLLDMVPSLGMS+TK   S S+VLEEK
Sbjct: 1141 PKS---------------KNSEKLEVMTDPLQAMLLDMVPSLGMSQTKSSSSSSSVLEEK 1200

Query: 1201 QPIHNNSQPAAKKKVSYKDVASELLKDW 1214
            QP+HNNS+P AKKKVSYKDVASELLKDW
Sbjct: 1201 QPVHNNSEP-AKKKVSYKDVASELLKDW 1209

BLAST of Sed0026596 vs. NCBI nr
Match: XP_023546648.1 (DNA ligase 4 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1957.6 bits (5070), Expect = 0.0e+00
Identity = 1013/1228 (82.49%), Postives = 1088/1228 (88.60%), Query Frame = 0

Query: 1    MTESMKFSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSPSDYFSAIRLILPSLDRERRT 60
            MTE +KFSVLSSLFDWIQRSKSS KKRSKFRKF+DTFCSP+DYFSAIRLILPSLDRER T
Sbjct: 1    MTELIKFSVLSSLFDWIQRSKSSAKKRSKFRKFIDTFCSPADYFSAIRLILPSLDRERGT 60

Query: 61   YGLKESVLATSLIDALGMSRDSDDALRLINWRKAGARAGANAGNFALLAAEVLQRRQGMT 120
            YGLKESVLAT LIDALGMSR+S+DALRLINWRK GA+ GAN GNFAL+AAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGMSRESEDALRLINWRKGGAKTGANVGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIKELNDLLDRLALSENRAEKTVVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180
            PGGL IKELNDLLDRLAL+ENRAEKT VLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS
Sbjct: 121  PGGLLIKELNDLLDRLALTENRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180

Query: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240
            IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK
Sbjct: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240

Query: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLSTRCILD 300
            KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVL+TRCILD
Sbjct: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLATRCILD 300

Query: 301  GEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDVLYVGDTSLIHRSLKERR 360
            GEMLVWDKSLNRFAEFGSNQEIA+AAKDGFDSNRQLCYVAFDVLYVGDTS+IHRSLKER 
Sbjct: 301  GEMLVWDKSLNRFAEFGSNQEIARAAKDGFDSNRQLCYVAFDVLYVGDTSVIHRSLKERH 360

Query: 361  ELLRNVVKPVKGRLEFLVPNGGLNNDCAGDPSWSMRAYNVDDVERFFKNTIENRDEGIVI 420
            ELLR VVKPVKG+LE LVPNGGLN+DCAGDPSWS+RAYNVDDVERFFK+TIENRDEGIVI
Sbjct: 361  ELLRGVVKPVKGQLEILVPNGGLNSDCAGDPSWSIRAYNVDDVERFFKSTIENRDEGIVI 420

Query: 421  KDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLADRPH 480
            KDL SKWEPGDRSGKWLKLKPDY+ AGSDLDALIIGGYYGSGRRGGEVAQFLMGLA+RP 
Sbjct: 421  KDLSSKWEPGDRSGKWLKLKPDYIHAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERPP 480

Query: 481  SNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKKTAPSFYQVTNNSKERPDV 540
            SNA+PRRFISFCRVGTGLTDEELD+VVNKLKPYFRKSEYP+KTAPSFYQVTNNSKERPDV
Sbjct: 481  SNAYPRRFISFCRVGTGLTDEELDSVVNKLKPYFRKSEYPRKTAPSFYQVTNNSKERPDV 540

Query: 541  WIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHS 600
            WIESPEKSIILSITSDIRTI SEVFAAPYSLRFPRI RVRYDKPWHECLDVQSFVELVHS
Sbjct: 541  WIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIQRVRYDKPWHECLDVQSFVELVHS 600

Query: 601  SNGTTQ-------GQDSKQKYIKSTGKGRKKNISAVPSHLLQTDISGIKEDSLIFSNMLF 660
            SNGTTQ        QDSK+KYI STGKGRKK++S VPSHLLQTDISGIK DSLIFSNMLF
Sbjct: 601  SNGTTQRGTNNSGWQDSKRKYINSTGKGRKKSVSVVPSHLLQTDISGIKGDSLIFSNMLF 660

Query: 661  YIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNIAADSKGIKYEAAKRHGDVIHYSW 720
            YIVNVPPT+SLDSLHKLIVENGGTFSMNLN+SV H++AADSKGIKYEAAKRHGDVIHYSW
Sbjct: 661  YIVNVPPTHSLDSLHKLIVENGGTFSMNLNNSVTHSVAADSKGIKYEAAKRHGDVIHYSW 720

Query: 721  VLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLLNNVSRSQ 780
            VLECCSQKKLLPL PK+FLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLL+NVSRS+
Sbjct: 721  VLECCSQKKLLPLMPKHFLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLLSNVSRSE 780

Query: 781  DVKAIDYYRGKYCPKHEWCIFVGCQIYFLPLRPSLKSDWDVLFELLIRRLKVEVSFRGGK 840
            DVKAIDYYR KYCPKHEWCIF GCQIYF PL+PSLKSDWD L EL +RRLKVEVSFRGGK
Sbjct: 781  DVKAIDYYREKYCPKHEWCIFSGCQIYFHPLKPSLKSDWDALLELSVRRLKVEVSFRGGK 840

Query: 841  VSDNHVSATHIVVFSMPGSPMGYETVLKRCSEAEKHVLLRDKIHIVAHQWLEDCLEMAHR 900
            VSD+ VSATHIVVF +PG P+ YE VLK C+EA+KHV+L+DK HIVAHQWLEDCLE A R
Sbjct: 841  VSDDRVSATHIVVFFIPGPPVKYEAVLKSCNEADKHVMLKDKTHIVAHQWLEDCLERAQR 900

Query: 901  LQEDAYSLNPNREKSIEKSNSGVDAAILPASENIENQQTSSPSKEYKNRESNKAASGKHM 960
            LQEDAYSLNPN +KSIEK NSG+ +    ASENI+NQQ  S SKEYK+   N++ASG+HM
Sbjct: 901  LQEDAYSLNPNTKKSIEKLNSGIGSETPSASENIDNQQIPSASKEYKDLGGNESASGQHM 960

Query: 961  LSYSTETTGEKKRGRPAARSLQKRTSGVEQVRRARARTTKGPAKISEVDSENSDHSDEK- 1020
            LS+ST+ +G KKRGRPAA S+QK  S +EQVRR   R TKGPAKI+EV+S++SDH++EK 
Sbjct: 961  LSHSTKRSGGKKRGRPAASSMQKGKSSIEQVRRVLPRRTKGPAKINEVESDSSDHTNEKT 1020

Query: 1021 ---IGNITTVRCENSTMNEFQTLENERMDEKKRGRPARNAPKRKAGVEPVRRTRARTAKR 1080
                GNI T+  E+   NEF+ LENE+  E KRG P RN  K KA VE VRRTRAR AK 
Sbjct: 1021 NAETGNIGTLCSESLGKNEFRMLENEKCVENKRGSPERNTQKGKAKVEQVRRTRARIAKG 1080

Query: 1081 SASISNIFSDNSDSTNETGMEKGNIGTLWEENSKTDEFKTLEH-DSKQKGKAIEQNDLAE 1140
              S    ++D   +  E  ME+GN+GT+ ++NSKT+E K LE  D KQKGKAIEQ D+A+
Sbjct: 1081 DGS---DYTDVKTNAEEAEMERGNVGTVCKDNSKTEELKMLEDCDWKQKGKAIEQIDVAD 1140

Query: 1141 PKSSAHEVDISERYKYKVEKSEKLEMMTDPLQAMLLDMVPSLGMSETK---SMSTVLEEK 1200
            PKS               E SEKLE+MTDPLQAMLLDMVPSLGMSETK   S S+VLEEK
Sbjct: 1141 PKSK------------NSENSEKLEVMTDPLQAMLLDMVPSLGMSETKSSSSSSSVLEEK 1200

Query: 1201 QPIHNNSQPAAKKKVSYKDVASELLKDW 1214
            QP        AKKKVSYKDVASELLKDW
Sbjct: 1201 QP--------AKKKVSYKDVASELLKDW 1205

BLAST of Sed0026596 vs. NCBI nr
Match: XP_022929706.1 (DNA ligase 4 [Cucurbita moschata] >XP_022929708.1 DNA ligase 4 [Cucurbita moschata])

HSP 1 Score: 1956.0 bits (5066), Expect = 0.0e+00
Identity = 1012/1228 (82.41%), Postives = 1089/1228 (88.68%), Query Frame = 0

Query: 1    MTESMKFSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSPSDYFSAIRLILPSLDRERRT 60
            MTE +KFSVLSSLFDWIQRSKSS KKRSKFRKF+DTFCSP+DYFSAIRLILPSLDRER T
Sbjct: 1    MTELIKFSVLSSLFDWIQRSKSSAKKRSKFRKFIDTFCSPADYFSAIRLILPSLDRERGT 60

Query: 61   YGLKESVLATSLIDALGMSRDSDDALRLINWRKAGARAGANAGNFALLAAEVLQRRQGMT 120
            YGLKESVLAT LIDALGMSR+S+DALRLINWRK GA+ GAN GNFAL+AAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGMSRESEDALRLINWRKGGAKTGANVGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIKELNDLLDRLALSENRAEKTVVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180
            PGGL IKELNDLLDRLALSENRAEKT VLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS
Sbjct: 121  PGGLLIKELNDLLDRLALSENRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180

Query: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240
            IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK
Sbjct: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240

Query: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLSTRCILD 300
            KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVL+TRCILD
Sbjct: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLATRCILD 300

Query: 301  GEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDVLYVGDTSLIHRSLKERR 360
            GEMLVWDKSLNRFAEFGSNQEIA+AAKDGFDSNRQLCYVAFDVLYVGDTS+IHRSLKER 
Sbjct: 301  GEMLVWDKSLNRFAEFGSNQEIARAAKDGFDSNRQLCYVAFDVLYVGDTSVIHRSLKERH 360

Query: 361  ELLRNVVKPVKGRLEFLVPNGGLNNDCAGDPSWSMRAYNVDDVERFFKNTIENRDEGIVI 420
            ELLR+VVKPVKG+LE LVPNGGLN+DCAGDPSWS+RAYNVDDVERFFK+TIENRDEGIVI
Sbjct: 361  ELLRDVVKPVKGQLEILVPNGGLNSDCAGDPSWSIRAYNVDDVERFFKSTIENRDEGIVI 420

Query: 421  KDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLADRPH 480
            KDL SKWEPGDRSGKWLKLKPDY+ AGSDLDALIIGGYYGSGRRGGEVAQFLMGLA+RP 
Sbjct: 421  KDLSSKWEPGDRSGKWLKLKPDYIHAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERPP 480

Query: 481  SNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKKTAPSFYQVTNNSKERPDV 540
            SNA+PRRFISFCRVGTGLTDEELD+VVNKLKPYFRKSEYP+KTAPSFYQVTNNSKERPDV
Sbjct: 481  SNAYPRRFISFCRVGTGLTDEELDSVVNKLKPYFRKSEYPRKTAPSFYQVTNNSKERPDV 540

Query: 541  WIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHS 600
            WIESPEKSIILSITSDIRTI SEVFAAPYSLRFPRI RVRYDKPWHECLDVQSFVELVHS
Sbjct: 541  WIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIHRVRYDKPWHECLDVQSFVELVHS 600

Query: 601  SNGTTQ-------GQDSKQKYIKSTGKGRKKNISAVPSHLLQTDISGIKEDSLIFSNMLF 660
            SNGTTQ        QDSK+KYI STGKGRKK++S VPSHLLQTDISGIK DSLIFSNMLF
Sbjct: 601  SNGTTQRGTNNSGWQDSKRKYINSTGKGRKKSVSVVPSHLLQTDISGIKGDSLIFSNMLF 660

Query: 661  YIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNIAADSKGIKYEAAKRHGDVIHYSW 720
            YIVNVPPT+SLDSLHKLIVENGGTFSMNLN+SV H++AADSKGIKYEAAKRHGDVIHYSW
Sbjct: 661  YIVNVPPTHSLDSLHKLIVENGGTFSMNLNNSVTHSVAADSKGIKYEAAKRHGDVIHYSW 720

Query: 721  VLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLLNNVSRSQ 780
            VLECCSQKKLLPL PK+FLFLS HSKKKLEEEIDEFSDSYFWDLELADLKQLL+NVSRS+
Sbjct: 721  VLECCSQKKLLPLMPKHFLFLSGHSKKKLEEEIDEFSDSYFWDLELADLKQLLSNVSRSE 780

Query: 781  DVKAIDYYRGKYCPKHEWCIFVGCQIYFLPLRPSLKSDWDVLFELLIRRLKVEVSFRGGK 840
            DVKAIDYYR KYCPKHEWCIF GCQIYF PL+PSLKSDWD L EL +RRLKVEVSFRGGK
Sbjct: 781  DVKAIDYYREKYCPKHEWCIFSGCQIYFHPLKPSLKSDWDALLELSVRRLKVEVSFRGGK 840

Query: 841  VSDNHVSATHIVVFSMPGSPMGYETVLKRCSEAEKHVLLRDKIHIVAHQWLEDCLEMAHR 900
            VSD+ VSATHIVVF +PG P+ YE VLK C+EA+KHV+L+DK HI+ HQWLEDCLE A R
Sbjct: 841  VSDDRVSATHIVVFCIPGPPVKYEAVLKSCNEADKHVMLKDKTHIIGHQWLEDCLERAQR 900

Query: 901  LQEDAYSLNPNREKSIEKSNSGVDAAILPASENIENQQTSSPSKEYKNRESNKAASGKHM 960
            LQEDAYSLNPN +KSIEK NSG+ +    ASENI+NQQ  SPSKEYK+   N++ASG+HM
Sbjct: 901  LQEDAYSLNPNTKKSIEKLNSGIGSETPSASENIDNQQIPSPSKEYKDLGGNESASGQHM 960

Query: 961  LSYSTETTGEKKRGRPAARSLQKRTSGVEQVRRARARTTKGPAKISEVDSENSDHSDEK- 1020
            LS+ST+ +G KKRGRPAA S+QK  S +EQVRR   R TKGPAKI+EV+S++SDH++EK 
Sbjct: 961  LSHSTKGSGGKKRGRPAASSMQKGKSSIEQVRRVLPRRTKGPAKINEVESDSSDHTNEKT 1020

Query: 1021 ---IGNITTVRCENSTMNEFQTLENERMDEKKRGRPARNAPKRKAGVEPVRRTRARTAKR 1080
                GNI T+  E+   NEF+ LENER  +KKRG PA+N  K KA VE VRRTRAR AK 
Sbjct: 1021 NAETGNIGTLCSESLGKNEFRMLENERPVKKKRGSPAKNTQKGKARVEQVRRTRARIAKG 1080

Query: 1081 SASISNIFSDNSDSTNETGMEKGNIGTLWEENSKTDEFKTLEH-DSKQKGKAIEQNDLAE 1140
              S    ++D   +  E  ME+GN+GT+ ++NSKT+E K LE  D KQKGKAIEQ D+A+
Sbjct: 1081 DGS---DYTDVKTNAEEAEMERGNVGTVCKDNSKTEELKMLEDCDWKQKGKAIEQIDVAD 1140

Query: 1141 PKSSAHEVDISERYKYKVEKSEKLEMMTDPLQAMLLDMVPSLGMSETK---SMSTVLEEK 1200
             KS               E SEKLE+MTDPLQAMLLDMVPSLGMSETK   S S+VLEEK
Sbjct: 1141 TKSK------------NSENSEKLEVMTDPLQAMLLDMVPSLGMSETKSSSSSSSVLEEK 1200

Query: 1201 QPIHNNSQPAAKKKVSYKDVASELLKDW 1214
            QP        AKKKVSYKDVASELLKDW
Sbjct: 1201 QP--------AKKKVSYKDVASELLKDW 1205

BLAST of Sed0026596 vs. NCBI nr
Match: XP_023546649.1 (DNA ligase 4 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1949.1 bits (5048), Expect = 0.0e+00
Identity = 1011/1228 (82.33%), Postives = 1086/1228 (88.44%), Query Frame = 0

Query: 1    MTESMKFSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSPSDYFSAIRLILPSLDRERRT 60
            MTE +KFSVLSSLFDWIQRSKSS KKRSKFRKF+DTFCSP+DYFSAIRLILPSLDRER T
Sbjct: 1    MTELIKFSVLSSLFDWIQRSKSSAKKRSKFRKFIDTFCSPADYFSAIRLILPSLDRERGT 60

Query: 61   YGLKESVLATSLIDALGMSRDSDDALRLINWRKAGARAGANAGNFALLAAEVLQRRQGMT 120
            YGLKESVLAT LIDALGMSR+S+DALRLINWRK GA+ GAN GNFAL+AAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGMSRESEDALRLINWRKGGAKTGANVGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIKELNDLLDRLALSENRAEKTVVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180
            PGGL IKELNDLLDRLAL+ENRAEKT VLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS
Sbjct: 121  PGGLLIKELNDLLDRLALTENRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180

Query: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240
            IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK
Sbjct: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240

Query: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLSTRCILD 300
            KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVL+TRCILD
Sbjct: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLATRCILD 300

Query: 301  GEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDVLYVGDTSLIHRSLKERR 360
            GEMLVWDKSLNRFAEFGSNQEIA+AAKDGFDSNRQLCYVAFDVLYVGDTS+IHRSLKER 
Sbjct: 301  GEMLVWDKSLNRFAEFGSNQEIARAAKDGFDSNRQLCYVAFDVLYVGDTSVIHRSLKERH 360

Query: 361  ELLRNVVKPVKGRLEFLVPNGGLNNDCAGDPSWSMRAYNVDDVERFFKNTIENRDEGIVI 420
            ELLR VVKPVKG+LE LVPNGGLN+DCAGDPSWS+RAYNVDDVERFFK+TIENRDEGIVI
Sbjct: 361  ELLRGVVKPVKGQLEILVPNGGLNSDCAGDPSWSIRAYNVDDVERFFKSTIENRDEGIVI 420

Query: 421  KDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLADRPH 480
            KDL SKWEPGDRSGKWLKLKPDY+ AGSDLDALIIGGYYGSGRRGGEVAQFLMGLA+RP 
Sbjct: 421  KDLSSKWEPGDRSGKWLKLKPDYIHAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERPP 480

Query: 481  SNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKKTAPSFYQVTNNSKERPDV 540
            SNA+PRRFISFCRVGTGLTDEELD+VVNKLKPYFR  EYP+KTAPSFYQVTNNSKERPDV
Sbjct: 481  SNAYPRRFISFCRVGTGLTDEELDSVVNKLKPYFR--EYPRKTAPSFYQVTNNSKERPDV 540

Query: 541  WIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHS 600
            WIESPEKSIILSITSDIRTI SEVFAAPYSLRFPRI RVRYDKPWHECLDVQSFVELVHS
Sbjct: 541  WIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIQRVRYDKPWHECLDVQSFVELVHS 600

Query: 601  SNGTTQ-------GQDSKQKYIKSTGKGRKKNISAVPSHLLQTDISGIKEDSLIFSNMLF 660
            SNGTTQ        QDSK+KYI STGKGRKK++S VPSHLLQTDISGIK DSLIFSNMLF
Sbjct: 601  SNGTTQRGTNNSGWQDSKRKYINSTGKGRKKSVSVVPSHLLQTDISGIKGDSLIFSNMLF 660

Query: 661  YIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNIAADSKGIKYEAAKRHGDVIHYSW 720
            YIVNVPPT+SLDSLHKLIVENGGTFSMNLN+SV H++AADSKGIKYEAAKRHGDVIHYSW
Sbjct: 661  YIVNVPPTHSLDSLHKLIVENGGTFSMNLNNSVTHSVAADSKGIKYEAAKRHGDVIHYSW 720

Query: 721  VLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLLNNVSRSQ 780
            VLECCSQKKLLPL PK+FLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLL+NVSRS+
Sbjct: 721  VLECCSQKKLLPLMPKHFLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLLSNVSRSE 780

Query: 781  DVKAIDYYRGKYCPKHEWCIFVGCQIYFLPLRPSLKSDWDVLFELLIRRLKVEVSFRGGK 840
            DVKAIDYYR KYCPKHEWCIF GCQIYF PL+PSLKSDWD L EL +RRLKVEVSFRGGK
Sbjct: 781  DVKAIDYYREKYCPKHEWCIFSGCQIYFHPLKPSLKSDWDALLELSVRRLKVEVSFRGGK 840

Query: 841  VSDNHVSATHIVVFSMPGSPMGYETVLKRCSEAEKHVLLRDKIHIVAHQWLEDCLEMAHR 900
            VSD+ VSATHIVVF +PG P+ YE VLK C+EA+KHV+L+DK HIVAHQWLEDCLE A R
Sbjct: 841  VSDDRVSATHIVVFFIPGPPVKYEAVLKSCNEADKHVMLKDKTHIVAHQWLEDCLERAQR 900

Query: 901  LQEDAYSLNPNREKSIEKSNSGVDAAILPASENIENQQTSSPSKEYKNRESNKAASGKHM 960
            LQEDAYSLNPN +KSIEK NSG+ +    ASENI+NQQ  S SKEYK+   N++ASG+HM
Sbjct: 901  LQEDAYSLNPNTKKSIEKLNSGIGSETPSASENIDNQQIPSASKEYKDLGGNESASGQHM 960

Query: 961  LSYSTETTGEKKRGRPAARSLQKRTSGVEQVRRARARTTKGPAKISEVDSENSDHSDEK- 1020
            LS+ST+ +G KKRGRPAA S+QK  S +EQVRR   R TKGPAKI+EV+S++SDH++EK 
Sbjct: 961  LSHSTKRSGGKKRGRPAASSMQKGKSSIEQVRRVLPRRTKGPAKINEVESDSSDHTNEKT 1020

Query: 1021 ---IGNITTVRCENSTMNEFQTLENERMDEKKRGRPARNAPKRKAGVEPVRRTRARTAKR 1080
                GNI T+  E+   NEF+ LENE+  E KRG P RN  K KA VE VRRTRAR AK 
Sbjct: 1021 NAETGNIGTLCSESLGKNEFRMLENEKCVENKRGSPERNTQKGKAKVEQVRRTRARIAKG 1080

Query: 1081 SASISNIFSDNSDSTNETGMEKGNIGTLWEENSKTDEFKTLEH-DSKQKGKAIEQNDLAE 1140
              S    ++D   +  E  ME+GN+GT+ ++NSKT+E K LE  D KQKGKAIEQ D+A+
Sbjct: 1081 DGS---DYTDVKTNAEEAEMERGNVGTVCKDNSKTEELKMLEDCDWKQKGKAIEQIDVAD 1140

Query: 1141 PKSSAHEVDISERYKYKVEKSEKLEMMTDPLQAMLLDMVPSLGMSETK---SMSTVLEEK 1200
            PKS               E SEKLE+MTDPLQAMLLDMVPSLGMSETK   S S+VLEEK
Sbjct: 1141 PKSK------------NSENSEKLEVMTDPLQAMLLDMVPSLGMSETKSSSSSSSVLEEK 1200

Query: 1201 QPIHNNSQPAAKKKVSYKDVASELLKDW 1214
            QP        AKKKVSYKDVASELLKDW
Sbjct: 1201 QP--------AKKKVSYKDVASELLKDW 1203

BLAST of Sed0026596 vs. ExPASy Swiss-Prot
Match: Q9LL84 (DNA ligase 4 OS=Arabidopsis thaliana OX=3702 GN=LIG4 PE=1 SV=1)

HSP 1 Score: 1387.9 bits (3591), Expect = 0.0e+00
Identity = 740/1257 (58.87%), Postives = 918/1257 (73.03%), Query Frame = 0

Query: 1    MTESMKFSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSPSDYFSAIRLILPSLDRERRT 60
            MTE +KFSVL SLF+WIQ+SK+S +KRSKFRKFLDT+C PSDYF A+RLI+PSLDRER +
Sbjct: 1    MTEEIKFSVLVSLFNWIQKSKTSSQKRSKFRKFLDTYCKPSDYFVAVRLIIPSLDRERGS 60

Query: 61   YGLKESVLATSLIDALGMSRDSDDALRLINWRKAG-ARAGANAGNFALLAAEVLQRRQGM 120
            YGLKESVLAT LIDALG+SRD+ DA+RL+NWRK G A+AGANAGNF+L+AAEVLQRRQGM
Sbjct: 61   YGLKESVLATCLIDALGISRDAPDAVRLLNWRKGGTAKAGANAGNFSLIAAEVLQRRQGM 120

Query: 121  TPGGLTIKELNDLLDRLALSENRAEKTVVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180
              GGLTIKELNDLLDRLA SENRAEKT+VLSTLIQKTNAQEMKW++ IILKDLKLG+SEK
Sbjct: 121  ASGGLTIKELNDLLDRLASSENRAEKTLVLSTLIQKTNAQEMKWVIRIILKDLKLGMSEK 180

Query: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAW 240
            SIF EFHPDAEDLFNVTCDLKLVCEKLRDR QRHKRQDIEVGKAVRPQLA R+ + NAAW
Sbjct: 181  SIFQEFHPDAEDLFNVTCDLKLVCEKLRDRHQRHKRQDIEVGKAVRPQLAMRIGDVNAAW 240

Query: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLSTRCIL 300
            KKLHGK+VV ECKFDGDRIQIHKNGT+IHYFSRNFLDHSEY H MSD+IVQN+L  +CIL
Sbjct: 241  KKLHGKDVVAECKFDGDRIQIHKNGTDIHYFSRNFLDHSEYAHAMSDLIVQNILVDKCIL 300

Query: 301  DGEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDVLYVGDTSLIHRSLKER 360
            DGEMLVWD SLNRFAEFGSNQEIAKAA++G DS++QLCYVAFDVLYVGDTS+IH+SLKER
Sbjct: 301  DGEMLVWDTSLNRFAEFGSNQEIAKAAREGLDSHKQLCYVAFDVLYVGDTSVIHQSLKER 360

Query: 361  RELLRNVVKPVKGRLEFLVPNGGLN-NDCAGDPSWSMRAYNVDDVERFFKNTIENRDEGI 420
             ELL+ VVKP+KGRLE LVP GGLN +  +G+PSWS+  +   DVERFFK T+ENRDEGI
Sbjct: 361  HELLKKVVKPLKGRLEVLVPEGGLNVHRPSGEPSWSIVVHAAADVERFFKETVENRDEGI 420

Query: 421  VIKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLADR 480
            V+KDL SKWEPGDRSGKW+KLKP+Y+RAG+DLD LIIGGYYGSGRRGGEVAQFL+ LADR
Sbjct: 421  VLKDLESKWEPGDRSGKWMKLKPEYIRAGADLDVLIIGGYYGSGRRGGEVAQFLVALADR 480

Query: 481  PHSNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKKTAPSFYQVTNNSKERP 540
              +N +PRRF+SFCRVGTGL+D+EL+ VV+KLKPYFRK+E+PKK  PSFYQVTN+SKERP
Sbjct: 481  AEANVYPRRFMSFCRVGTGLSDDELNTVVSKLKPYFRKNEHPKKAPPSFYQVTNHSKERP 540

Query: 541  DVWIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELV 600
            DVWI+SPEKSIILSITSDIRTI SEVF APYSLRFPRID+VRYDKPWHECLDVQ+FVELV
Sbjct: 541  DVWIDSPEKSIILSITSDIRTIRSEVFVAPYSLRFPRIDKVRYDKPWHECLDVQAFVELV 600

Query: 601  HSSNGTTQGQDSKQ------KYIKSTGKGRKKNISAVPSHLLQTDISGIKEDSLIFSNML 660
            +SSNGTTQ Q   +      K  KS+ +G KKN+S VPS  +QTD+S IK  + IFSNM+
Sbjct: 601  NSSNGTTQKQKESESTQDNPKVNKSSKRGEKKNVSLVPSQFIQTDVSDIKGKTSIFSNMI 660

Query: 661  FYIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNIAADSKGIKYEAAKRHGDVIHYS 720
            FY VNVP ++SL++ HK++VENGG FSMNLN+SV H IAA+S GIKY+AAKR  DVIH+S
Sbjct: 661  FYFVNVPRSHSLETFHKMVVENGGKFSMNLNNSVTHCIAAESSGIKYQAAKRQRDVIHFS 720

Query: 721  WVLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLLNNVSRS 780
            WVL+CCS+ K+LPL PKYFL L+D S+ KL+++IDEFSDSY+WDL+L  LKQ+L+N  +S
Sbjct: 721  WVLDCCSRNKMLPLLPKYFLHLTDASRTKLQDDIDEFSDSYYWDLDLEGLKQVLSNAKQS 780

Query: 781  QDVKAIDYYRGKYCPKHEWCIFVGCQIYFLPLRPSLKSDWDVLFELLIRRLKVEVSFRGG 840
            +D K+IDYY+ K CP+  W   + C +YF P   +L ++ + L  ++ +RL +EV   GG
Sbjct: 781  EDSKSIDYYKKKLCPEKRWSCLLSCCVYFYPYSQTLSTEEEALLGIMAKRLMLEVLMAGG 840

Query: 841  KVSDNHVSATHIVVFSMPGSPMGYETVLKRCSEAEKHVLLRDKIHIVAHQWLEDCLEMAH 900
            KVS+N   A+H+VV +M   P+ +  V K  SE EK +LL+ ++H+V+  WLE+ L+   
Sbjct: 841  KVSNNLAHASHLVVLAMAEEPLDFTLVSKSFSEMEKRLLLKKRLHVVSSHWLEESLQREE 900

Query: 901  RLQEDAYSLNPNREKSIEKSNSGVDAAILPASENIENQQTSSPSKEYKNRESNKAASGKH 960
            +L ED Y+L P   K +E+S    D      SE+   +  S  S + K   S+K A    
Sbjct: 901  KLCEDVYTLRP---KYMEES----DTEESDKSEHDTTEVASQGSAQTKEPASSKIAI--- 960

Query: 961  MLSYSTETTGEKKRGRPAARSLQKRTSGVEQVRRARARTTKGPAKISEVDSENSDHSDEK 1020
                         RGR   R++++  S    ++R + R  K P+KIS  ++E SD S+EK
Sbjct: 961  ----------TSSRGRSNTRAVKRGRSSTNSLQRVQRRRGKQPSKISGDETEESDASEEK 1020

Query: 1021 IG-NITTVRCENSTMNEFQTLENERMDEKKRGRPARNAPKRKAGVEPVRRTRARTAKRSA 1080
            +   ++ +  E  +  E Q           RG+ A+    R    + V+  R+R  K++A
Sbjct: 1021 VSTRLSDIAEETDSFGEAQR-------NSSRGKCAKRGKSRVGQTQRVQ--RSRRGKKAA 1080

Query: 1081 SISNIFSDNSDSTN-----ETGMEKGNIGTLWEENSKTDEFKTLEH-DSKQKGKAIEQND 1140
             I    SD +D  +         E+GN      EN +T E    ++ +S+Q+   +   +
Sbjct: 1081 KIGGDESDENDELDGNNNVSADAEEGNAAGRSVENEETREPDIAKYTESQQRDNTVAVEE 1140

Query: 1141 LAEPKSSAHEVDISERYKYKVEKSEKLEMMTDPLQAMLLDM--VPSLGMSETKSMSTVLE 1200
              +   +A         K +++  EKL++  DPLQAML+ M  +PS   +ET + +T   
Sbjct: 1141 ALQDSRNA---------KTEMDMKEKLQIHEDPLQAMLMKMFPIPSQKTTETSNRTTGEY 1200

Query: 1201 EKQPIHNNSQPA---------------------------AKKKVSYKDVASELLKDW 1214
             K  +    + +                            KKKVSY+DVA ELLKDW
Sbjct: 1201 RKANVSGECESSEKRKLDAETDNTSVNAGAESDVVPPLVKKKKVSYRDVAGELLKDW 1219

BLAST of Sed0026596 vs. ExPASy Swiss-Prot
Match: Q7X7E9 (Putative DNA ligase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=LIG4 PE=2 SV=2)

HSP 1 Score: 1052.4 bits (2720), Expect = 4.0e-306
Identity = 634/1422 (44.59%), Postives = 831/1422 (58.44%), Query Frame = 0

Query: 1    MTESMKFSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSP---SDYFSAIRLILPSLDRE 60
            M  +++F +L ++F  + R +++ KKR++ R  LD    P    DYFSA+RL+LP LDRE
Sbjct: 1    MAATVRFGLLVAMFQAMSRDRTAAKKRARLRALLDRAYGPGGRDDYFSALRLVLPGLDRE 60

Query: 61   RRTYGLKESVLATSLIDALGMSRDSDDALRLINWRKAGARAGANAGNFALLAAEVLQRRQ 120
            R +YGLKE+ LA+ L+DALG+++DS DA+RL NWR+ G     NAGNFAL+AAEVLQRRQ
Sbjct: 61   RGSYGLKEAALASVLVDALGIAKDSPDAVRLTNWRRGG--GFRNAGNFALVAAEVLQRRQ 120

Query: 121  GMTPGGLTIKELNDLLDRLALSEN--------------RAEKTVVLSTLIQKTNAQEMKW 180
            GMT GGLTIKE+ND LDRLA +EN              R+EK  +LS+LI+KTNA EMKW
Sbjct: 121  GMTSGGLTIKEVNDALDRLAATENSVCLESEKLLLILDRSEKASILSSLIKKTNALEMKW 180

Query: 181  IVMIILKDLKLGVSEKSIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKA 240
            ++MIILKDLKLG+SEKS+FHEFHPDAEDLFNVTCDL+LVCEKL DR QRHKRQDIEVGKA
Sbjct: 181  LLMIILKDLKLGISEKSVFHEFHPDAEDLFNVTCDLRLVCEKLNDRSQRHKRQDIEVGKA 240

Query: 241  VRPQLASRVSNANAAWKKLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHG 300
            VRPQL+ RV+NA++AWKKLHGK+VV ECKFDGDRIQIHKNG EIH+FSR+FLDHSEY  G
Sbjct: 241  VRPQLSMRVNNASSAWKKLHGKQVVAECKFDGDRIQIHKNGEEIHFFSRSFLDHSEYAPG 300

Query: 301  MSDVIVQNVLSTRCILDGEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLC------ 360
            MS VI++N+L  RCILDGEMLVWD  LNRFAEFGSNQEIAKAAK+G +++RQLC      
Sbjct: 301  MSKVIIENILVDRCILDGEMLVWDTVLNRFAEFGSNQEIAKAAKEGLETDRQLCCILEWI 360

Query: 361  -------------------------YVAFDVLYVGDTSLIHRSLKERRELLRNVVKPVKG 420
                                     YVAFD+LY GDTS+IH+SL ER E+L+ VV+P+KG
Sbjct: 361  ASYVLALCVNILSCQDFFFSNKRESYVAFDILYAGDTSVIHQSLTERHEILQKVVRPLKG 420

Query: 421  RLEFLVPNGGLN-NDCAGDPSWSMRAYNVDDVERFFKNTIEN------------------ 480
             LE LVP GGLN +    +P WS+ A+++DDVE+FFK+T++N                  
Sbjct: 421  HLEILVPTGGLNIHRPPDEPCWSILAHSLDDVEKFFKDTVDNRFFPTSPNTDELSNLSIS 480

Query: 481  ----------------RDEGIVIKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGG 540
                            R+EGI++KDL SKWEPGDRSGKWLKLKPDY+ AG+DLD +IIGG
Sbjct: 481  LMIQFRFSDYSICYVLREEGIILKDLESKWEPGDRSGKWLKLKPDYIHAGADLDVIIIGG 540

Query: 541  YYGSGRRGGEVAQFLMGLADRPHSNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKS 600
            YYGSGRRGGEVAQFL+GLA     N++P+RF+SFCRVGTGL+DEELDA+V KLKP+FRK+
Sbjct: 541  YYGSGRRGGEVAQFLVGLAVPSDDNSYPKRFLSFCRVGTGLSDEELDALVTKLKPHFRKN 600

Query: 601  EYPKKTAPSFYQVTNNSKERPDVWIESPEK-----------------SIILSITSDIRTI 660
            EYPKK  P FY+VTN+SKERPDVWIESP+K                 S+I+SITSDIRTI
Sbjct: 601  EYPKK-PPRFYEVTNHSKERPDVWIESPDKQSVRFRKHLIYCITKCRSVIISITSDIRTI 660

Query: 661  NSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHSSNGTTQGQDSKQKYIKSTGK 720
             SEVFAAPYSLRFPRI R+RYDKPWHECLDVQ+FV++VHSSNGTT               
Sbjct: 661  KSEVFAAPYSLRFPRIQRLRYDKPWHECLDVQAFVDIVHSSNGTT--------------- 720

Query: 721  GRKKNISAVPSHLLQTDISGIKEDSLIFSNMLFYIVNVPPTYSLDSLHKLIVENGGTFSM 780
                       H    D + +K                             V+NGG+FSM
Sbjct: 721  -----------HRAADDDNDLKN----------------------------VKNGGSFSM 780

Query: 781  NLNSSVKHNIAADSKGIKYEAAKRHGDVIHYSWVLECCSQKKLLPLQPKYFLFLSDHSKK 840
            NLN SV H IAA+ K      A R G +IHYSW+L+CC +K+LL LQPK+   L      
Sbjct: 781  NLNDSVTHCIAAEKK------ATRQGRIIHYSWILDCCKEKRLLHLQPKFDPCL--QPMH 840

Query: 841  KLEEEIDEFSDSYFWDLELADLKQLLNNVSRS-QDVKAIDYYRGKYCPKHEWCIFVGCQI 900
            K  EEID ++D ++WD++++DLKQ+ +N+ R+  D   + +Y+ KYC    +C F GC +
Sbjct: 841  KFPEEIDSYADYFYWDIDISDLKQIFSNMDRAVVDSNMVHHYKKKYCADERFCFFQGCCV 900

Query: 901  YFLPLRPSLKSDWDVLFELLIRRLKVEVSFRGGKVSDNHVSATHIVVFSMPGSPMGYETV 960
            YF    P + +D++V+ +L ++R+K +++  GG+V      ATH+++ S+      ++ +
Sbjct: 901  YFYH-APLVNADYNVISDLALKRVKQDLTMHGGQVCSILAPATHLIIVSVL-QAYNFDML 960

Query: 961  LKRCSEAEKHVLLRDKIHIVAHQWLEDCLEMAHRLQEDAYSLNPNREKSIEKSNSGVDAA 1020
             K    AE+  L   ++ +V+++WLED +E   RL E  YSL P+  + IE   S  +  
Sbjct: 961  YKSLPPAERRYLHDKRLQVVSNKWLEDSVEKQTRLPETTYSLKPDTLEEIEIERS--EET 1020

Query: 1021 ILPASENIENQQTSSPSKEYKNRESNKAASGKHMLSYSTETTGEKKRGRPAARSLQKRTS 1080
            + P ++ +E  + +  S              KH           +KRGRP++ + +    
Sbjct: 1021 VQPCNDKLEENEKADTSHV------------KH---------APRKRGRPSSSASRTAKP 1080

Query: 1081 GVEQVRRARARTTKGPAKISEVDSENSDHS----DEKI---GNITTVRCENSTMNEFQT- 1140
                VRR RAR     AKI +V+ E SDH     D++I    NI+ +  ++   ++    
Sbjct: 1081 APRPVRRTRARRGNQHAKIDDVEPEESDHGETGLDDQIPDTDNISKMEVDSFDKDQVSAR 1140

Query: 1141 ------------------------------LENERMDEKKRGRPARNAPKRKAGVEP--- 1200
                                           +++R+D     +   ++  R  G  P   
Sbjct: 1141 PVRRTRARRGKQHAKIDYGQSEESDPGETGQDDQRLDADYISKMEEDSSDRDQGAHPTAP 1200

Query: 1201 --VRRTRARTAKRSASISNIFSDNSDSTNETGMEKGNIGTLWEENSKTDEFKTLEHD--- 1214
              VRR+RA+  K  A I     D      ETG +   +    +  SK +E     HD   
Sbjct: 1201 RVVRRSRAQRGKWLAKI-----DRETGPGETGQDDKKLNA--DSISKMEEH---AHDKDQ 1260

BLAST of Sed0026596 vs. ExPASy Swiss-Prot
Match: Q90YB1 (DNA ligase 4 OS=Gallus gallus OX=9031 GN=LIG4 PE=2 SV=2)

HSP 1 Score: 411.4 bits (1056), Expect = 3.6e-113
Identity = 285/872 (32.68%), Postives = 451/872 (51.72%), Query Frame = 0

Query: 7   FSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCS------------PSDYFSAIRLILPSL 66
           F+ L S  + IQ  KS  +K   F+ FLD++                 ++ A+RLILP L
Sbjct: 21  FADLCSTLERIQTCKSRPEKTKYFKDFLDSWRKFHSALHQKEKDVTDSFYPAMRLILPQL 80

Query: 67  DRERRTYGLKESVLATSLIDALGMSRDSDDALRLINWRKAGARAGANAGNFALLAAEVLQ 126
           +RER  YG+KE++LA   I+ L + +D  DA++L+N+R      G +AG+FA++A  VL+
Sbjct: 81  ERERMAYGIKETMLAKLYIELLNLPKDGKDAVKLLNYRTPTGSRG-DAGDFAMIAYFVLK 140

Query: 127 RRQGMTPGGLTIKELNDLLDRLALSENRAEKTVVLSTLIQ---KTNAQEMKWIVMIILKD 186
            R     G LT++++N+LLD +A +     K +V  +L+Q   ++ A E KW++ +I+KD
Sbjct: 141 PRSPKR-GRLTVEQVNELLDAIANNNAAKNKGLVKKSLLQLITQSTALEQKWLIRMIIKD 200

Query: 187 LKLGVSEKSIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASR 246
           LKLGVS+++IF  FHPDA +L NVT DL+ VC +L D         I +  A +P LA+ 
Sbjct: 201 LKLGVSQQTIFSIFHPDAAELHNVTTDLEKVCRQLHDPSVSLSDVSIMLFSAFKPMLAA- 260

Query: 247 VSNANAAWKKLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHS-EYG----HGMSD 306
           +++     K+++ +   +E K DG+R+Q+HK+G    YFSRN  D++ ++G     G   
Sbjct: 261 IADVQQIEKQMNNQVFYIETKLDGERMQMHKDGDVYKYFSRNGFDYTQQFGASPVDGSLT 320

Query: 307 VIVQNVLST---RCILDGEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDV 366
             + NV  +    CILDGEM+ ++     F + G+  +I +  +   DS+ Q C+  FDV
Sbjct: 321 PFIHNVFKSDIQNCILDGEMMAYNPETQTFMQKGNKFDIKRMVE---DSDLQTCFCVFDV 380

Query: 367 LYVGDTSLIHRSLKERRELLRNVVKPVKGRLEFLVPNGGLNNDCAGDPSWSMRAYNVDDV 426
           L + D  L H SL +R ++L NV  P+ GR+  +                   A    +V
Sbjct: 381 LMINDQKLAHESLSKRYKILSNVFTPLTGRIHVV---------------HKKSARTRKEV 440

Query: 427 ERFFKNTIENRDEGIVIKDLGSKWEPGDRSGKWLKLKPDYVRAGSD-LDALIIGGYYGSG 486
                  I+NR+EGI++KD  S ++P  R   WLK+KP+YV    D LD LI+GGY+G G
Sbjct: 441 IDALNEAIDNREEGIMVKDPMSTYKPDKRGEGWLKIKPEYVNGLMDELDLLIVGGYWGKG 500

Query: 487 RRGGEVAQFLMGLADRPHSNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKK 546
            RGG ++ FL  +A+ P  N  P  F S CRVG+G T +EL  +  KL  +++   Y +K
Sbjct: 501 SRGGMMSHFLCAVAETPAPNEKPTVFHSICRVGSGYTMKELYDLGLKLAKHWK--PYNRK 560

Query: 547 TAPSFYQVTNNSKERPDVWIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYD 606
             P          E+P+++IE P  S+I+ I +    +NS+++    +LRFPRI+++R D
Sbjct: 561 DPPCNILC---GTEKPEMYIE-PCNSVIVQIKA-AEIVNSDMYKTDCTLRFPRIEKIRED 620

Query: 607 KPWHECLDVQSFVELVHSSNGTTQGQDSKQKYIKSTGKGRKKNISAVP---------SHL 666
           K W+EC+ +     L   + G      SK  YI    + ++K    VP            
Sbjct: 621 KEWYECMTLDMLEHLRSRAEGKLA---SKHLYIDEYDEPQEKKRRTVPKVKKVIGIAEQF 680

Query: 667 LQTDISGIKEDSLIFSNMLFYIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNI-AA 726
              D+S + + S +F ++ F ++     YS   L   I E GG+   N        I  A
Sbjct: 681 KAPDLSNVNKVSSMFEDVEFCVMTGMGRYSKSELESRIAECGGSVVQNPGPDTYCVIVGA 740

Query: 727 DSKGIKYEAAKRHGDVIHYSWVLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDS 786
           ++  +K   A    DV+   W+L+C   K L+P QP + + +S  +++    E D + DS
Sbjct: 741 ENVRVKNIIASNKYDVVKAEWLLQCFQSKMLVPWQPAFMIHMSPETREHFAREYDCYGDS 800

Query: 787 YFWDLELADLKQLLNNVSRSQDV------KAIDYYRGKYCPKHEWCIFVGCQIYF----L 835
           Y  D ++A LK++ + V  ++ +      +  + Y    C   + CIF G  IY     +
Sbjct: 801 YTADTDVAQLKEVFSRVKDNKKMPLDLIAELEERYSWNSC---KLCIFRGNTIYVDYYAI 853

BLAST of Sed0026596 vs. ExPASy Swiss-Prot
Match: Q5R6L3 (DNA ligase 4 OS=Pongo abelii OX=9601 GN=DNL4 PE=2 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.8e-112
Identity = 265/801 (33.08%), Postives = 434/801 (54.18%), Query Frame = 0

Query: 7   FSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSPSD------------YFSAIRLILPSL 66
           F+ L S  + IQ+SK   +K   FR+FLD++    D            ++ A+RLILP L
Sbjct: 16  FADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDAFHKNQKDVTDSFYPAMRLILPQL 75

Query: 67  DRERRTYGLKESVLATSLIDALGMSRDSDDALRLINWRKAGARAGANAGNFALLAAEVLQ 126
           +RER  YG+KE++LA   I+ L + RD  DAL+L+N+R      G +AG+FA++A  VL+
Sbjct: 76  ERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHG-DAGDFAMIAYFVLK 135

Query: 127 RRQGMTPGGLTIKELNDLLDRLALSENRAEKTVVLSTLIQ---KTNAQEMKWIVMIILKD 186
            R  +  G LTI+++NDLLD +A + +   K ++  +L+Q   +++A E KW++ +I+KD
Sbjct: 136 PR-CLQKGSLTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKD 195

Query: 187 LKLGVSEKSIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASR 246
           LKLGVS+++IF  FH DA +L NVT DL+ VC +L D         I +  A +P LA+ 
Sbjct: 196 LKLGVSQQTIFSVFHSDAVELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAA- 255

Query: 247 VSNANAAWKKLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHS-EYG----HGMSD 306
           +++     K +  +   +E K DG+R+Q+HK+G    YFSRN  +++ ++G     G   
Sbjct: 256 IADIEHIEKDMKHQSFYIETKLDGERMQMHKDGDVYKYFSRNGYNYTDQFGASPTEGSLT 315

Query: 307 VIVQNVLSTR---CILDGEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDV 366
             + N   T    CILDGEM+ ++ +   F + G+  +I +  +   DS+ Q CY  FDV
Sbjct: 316 PFIHNAFKTDIQICILDGEMMAYNPNTQTFMQKGTKFDIKRMVE---DSDLQTCYCVFDV 375

Query: 367 LYVGDTSLIHRSLKERRELLRNVVKPVKGRLEFLVPNGGLNNDCAGDPSWSMRAYNVDDV 426
           L V +  L H +L++R E+L ++  P+ GR+E +                  +A+  ++V
Sbjct: 376 LMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIV---------------QKTQAHTKNEV 435

Query: 427 ERFFKNTIENRDEGIVIKDLGSKWEPGDRSGKWLKLKPDYVRAGSD-LDALIIGGYYGSG 486
                  I+ R+EGI+IK   S ++P  R   WLK+KP+YV    D LD LI+GGY+G G
Sbjct: 436 IDALNEAIDKREEGIMIKQPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKG 495

Query: 487 RRGGEVAQFLMGLADRPHSNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKK 546
            RGG ++ FL  +A++P     P  F +  RVG+G T +EL  +  KL  Y++   + KK
Sbjct: 496 SRGGMMSHFLCAVAEKPPPGEKPSVFHTLSRVGSGCTMKELYDLGLKLAKYWK--PFHKK 555

Query: 547 TAPSFYQVTNNSKERPDVWIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYD 606
             PS         E+P+V+IE P  S+I+ I +    + S+++    +LRFPRI+++R D
Sbjct: 556 APPSSILC---GTEKPEVYIE-PCNSVIVQIKA-AEIVPSDMYKTGCTLRFPRIEKIRDD 615

Query: 607 KPWHECLDVQSFVELVHSSNGTTQ------GQDSKQKYIKSTGKGRKKNISAVPSHLLQT 666
           K WHEC+ +    +L   ++G         G D + +  K     + K +  +  HL   
Sbjct: 616 KEWHECMTLDDLEQLRGKASGKLASKHFYVGGDDEPQEKKRKAAPKMKKVIGIIEHLKAP 675

Query: 667 DISGIKEDSLIFSNMLFYIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNIAADSKG 726
           +++ + + S IF ++ F +++   +     L   I E GG    N      + + A SK 
Sbjct: 676 NLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDT-YCVIAGSKN 735

Query: 727 IKYE---AAKRHGDVIHYSWVLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDSY 775
           I+ +    + +H DV+  +W+LEC   K  +P QP + + +   +K+    E D + DSY
Sbjct: 736 IRVKNIILSNKH-DVVKPAWLLECFKTKSFVPWQPHFMIHMCPSTKEHFAREYDCYGDSY 786

BLAST of Sed0026596 vs. ExPASy Swiss-Prot
Match: P49917 (DNA ligase 4 OS=Homo sapiens OX=9606 GN=LIG4 PE=1 SV=2)

HSP 1 Score: 405.2 bits (1040), Expect = 2.6e-111
Identity = 261/801 (32.58%), Postives = 434/801 (54.18%), Query Frame = 0

Query: 7   FSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSPSD------------YFSAIRLILPSL 66
           F+ L S  + IQ+SK   +K   FR+FLD++    D            ++ A+RLILP L
Sbjct: 16  FADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHKNHKDVTDSFYPAMRLILPQL 75

Query: 67  DRERRTYGLKESVLATSLIDALGMSRDSDDALRLINWRKAGARAGANAGNFALLAAEVLQ 126
           +RER  YG+KE++LA   I+ L + RD  DAL+L+N+R      G +AG+FA++A  VL+
Sbjct: 76  ERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHG-DAGDFAMIAYFVLK 135

Query: 127 RRQGMTPGGLTIKELNDLLDRLALSENRAEKTVVLSTLIQ---KTNAQEMKWIVMIILKD 186
            R  +  G LTI+++NDLLD +A + +   K ++  +L+Q   +++A E KW++ +I+KD
Sbjct: 136 PR-CLQKGSLTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKD 195

Query: 187 LKLGVSEKSIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASR 246
           LKLGVS+++IF  FH DA +L NVT DL+ VC +L D         I +  A +P LA+ 
Sbjct: 196 LKLGVSQQTIFSVFHNDAAELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAA- 255

Query: 247 VSNANAAWKKLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHS-EYG----HGMSD 306
           +++     K +  +   +E K DG+R+Q+HK+G    YFSRN  +++ ++G     G   
Sbjct: 256 IADIEHIEKDMKHQSFYIETKLDGERMQMHKDGDVYKYFSRNGYNYTDQFGASPTEGSLT 315

Query: 307 VIVQNVLSTR---CILDGEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDV 366
             + N        CILDGEM+ ++ +   F + G+  +I +  +   DS+ Q CY  FDV
Sbjct: 316 PFIHNAFKADIQICILDGEMMAYNPNTQTFMQKGTKFDIKRMVE---DSDLQTCYCVFDV 375

Query: 367 LYVGDTSLIHRSLKERRELLRNVVKPVKGRLEFLVPNGGLNNDCAGDPSWSMRAYNVDDV 426
           L V +  L H +L++R E+L ++  P+ GR+E +                  +A+  ++V
Sbjct: 376 LMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIV---------------QKTQAHTKNEV 435

Query: 427 ERFFKNTIENRDEGIVIKDLGSKWEPGDRSGKWLKLKPDYVRAGSD-LDALIIGGYYGSG 486
                  I+ R+EGI++K   S ++P  R   WLK+KP+YV    D LD LI+GGY+G G
Sbjct: 436 IDALNEAIDKREEGIMVKQPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKG 495

Query: 487 RRGGEVAQFLMGLADRPHSNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKK 546
            RGG ++ FL  +A++P     P  F +  RVG+G T +EL  +  KL  Y++   + +K
Sbjct: 496 SRGGMMSHFLCAVAEKPPPGEKPSVFHTLSRVGSGCTMKELYDLGLKLAKYWK--PFHRK 555

Query: 547 TAPSFYQVTNNSKERPDVWIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYD 606
             PS         E+P+V+IE P  S+I+ I +    + S+++    +LRFPRI+++R D
Sbjct: 556 APPSSILC---GTEKPEVYIE-PCNSVIVQIKA-AEIVPSDMYKTGCTLRFPRIEKIRDD 615

Query: 607 KPWHECLDVQSFVELVHSSNGTTQ------GQDSKQKYIKSTGKGRKKNISAVPSHLLQT 666
           K WHEC+ +    +L   ++G         G D + +  K     + K +  +  HL   
Sbjct: 616 KEWHECMTLDDLEQLRGKASGKLASKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAP 675

Query: 667 DISGIKEDSLIFSNMLFYIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNIAADSKG 726
           +++ + + S IF ++ F +++   +     L   I E GG    N      + + A S+ 
Sbjct: 676 NLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDT-YCVIAGSEN 735

Query: 727 IKYE---AAKRHGDVIHYSWVLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDSY 775
           I+ +    + +H DV+  +W+LEC   K  +P QP++ + +   +K+    E D + DSY
Sbjct: 736 IRVKNIILSNKH-DVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSY 786

BLAST of Sed0026596 vs. ExPASy TrEMBL
Match: A0A6J1K974 (DNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111491798 PE=3 SV=1)

HSP 1 Score: 1963.3 bits (5085), Expect = 0.0e+00
Identity = 1014/1228 (82.57%), Postives = 1094/1228 (89.09%), Query Frame = 0

Query: 1    MTESMKFSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSPSDYFSAIRLILPSLDRERRT 60
            MTE +KFSVLSSLFDWIQRSKSS KKRSKFRKF+DTFCSP+DYFSAIRLILPSLDRER T
Sbjct: 1    MTELIKFSVLSSLFDWIQRSKSSAKKRSKFRKFIDTFCSPADYFSAIRLILPSLDRERGT 60

Query: 61   YGLKESVLATSLIDALGMSRDSDDALRLINWRKAGARAGANAGNFALLAAEVLQRRQGMT 120
            YGLKESVLAT LIDALGMSR+S+DALRLINWRK GA+ GAN GNFAL+AAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGMSRESEDALRLINWRKGGAKTGANVGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIKELNDLLDRLALSENRAEKTVVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180
            PGGL IKELNDLLDRLALSENRAEKT VLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS
Sbjct: 121  PGGLLIKELNDLLDRLALSENRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180

Query: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240
            IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK
Sbjct: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240

Query: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLSTRCILD 300
            KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVL+TRCILD
Sbjct: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLATRCILD 300

Query: 301  GEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDVLYVGDTSLIHRSLKERR 360
            GEMLVWDKSLNRFAEFGSNQEIA+AAKDGFDSNRQLCYVAFDVLYVGDTS+IHRSLKER 
Sbjct: 301  GEMLVWDKSLNRFAEFGSNQEIARAAKDGFDSNRQLCYVAFDVLYVGDTSVIHRSLKERH 360

Query: 361  ELLRNVVKPVKGRLEFLVPNGGLNNDCAGDPSWSMRAYNVDDVERFFKNTIENRDEGIVI 420
            ELLR+VVKPVKG+LE LVPNGGLN+DCAGDP WS+RAYNVDDVERFFK+TIENRDEGIVI
Sbjct: 361  ELLRDVVKPVKGQLEILVPNGGLNSDCAGDPPWSIRAYNVDDVERFFKSTIENRDEGIVI 420

Query: 421  KDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLADRPH 480
            KDL SKWEPGDRSGKWLKLKPDY+ AGSDLDALIIGGYYGSGRRGGEVAQFLMGLA+ P 
Sbjct: 421  KDLSSKWEPGDRSGKWLKLKPDYIHAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAEHPP 480

Query: 481  SNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKKTAPSFYQVTNNSKERPDV 540
            SNA+PRRFISFCRVGTGLTDEELD+VVNKLKPYFRKSEYP+KTAPSFYQVTNNSKERPDV
Sbjct: 481  SNAYPRRFISFCRVGTGLTDEELDSVVNKLKPYFRKSEYPRKTAPSFYQVTNNSKERPDV 540

Query: 541  WIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHS 600
            WIESPEKSIILSITSDIRTI SEVFAAPYSLRFPRI RVRYDKPWHECLDVQSFVELVHS
Sbjct: 541  WIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIHRVRYDKPWHECLDVQSFVELVHS 600

Query: 601  SNGTTQ-------GQDSKQKYIKSTGKGRKKNISAVPSHLLQTDISGIKEDSLIFSNMLF 660
            SNGTTQ        QDSK+K I STGKGRKK++S VPSHLLQTDISGIK DSLIFSNMLF
Sbjct: 601  SNGTTQRGTNNSGWQDSKRKDINSTGKGRKKSVSVVPSHLLQTDISGIKGDSLIFSNMLF 660

Query: 661  YIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNIAADSKGIKYEAAKRHGDVIHYSW 720
            YIVNVPPT+SLDSLHKLIVENGGTFSMNLN+SV H++AADSKGIKYEAAKRHGDVIHYSW
Sbjct: 661  YIVNVPPTHSLDSLHKLIVENGGTFSMNLNNSVTHSVAADSKGIKYEAAKRHGDVIHYSW 720

Query: 721  VLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLLNNVSRSQ 780
            VLECCSQKKLLPL PK+FLFLS HSKKKLEEEIDEFSDSYFWDLELADLKQLL+NVSRS+
Sbjct: 721  VLECCSQKKLLPLMPKHFLFLSGHSKKKLEEEIDEFSDSYFWDLELADLKQLLSNVSRSE 780

Query: 781  DVKAIDYYRGKYCPKHEWCIFVGCQIYFLPLRPSLKSDWDVLFELLIRRLKVEVSFRGGK 840
            DVKAIDYYR KYCPKHEWCIF GCQIYF PL+PSLKSDWD L EL +RRLKVEVSFRGGK
Sbjct: 781  DVKAIDYYREKYCPKHEWCIFSGCQIYFHPLKPSLKSDWDSLLELSVRRLKVEVSFRGGK 840

Query: 841  VSDNHVSATHIVVFSMPGSPMGYETVLKRCSEAEKHVLLRDKIHIVAHQWLEDCLEMAHR 900
            VSD+ VSATHIVVF +PG P+  E VLK C+EA+KHV+L+DK HIVAHQWLEDCLE A R
Sbjct: 841  VSDDRVSATHIVVFCIPGPPVKCEAVLKSCNEADKHVMLKDKTHIVAHQWLEDCLERAQR 900

Query: 901  LQEDAYSLNPNREKSIEKSNSGVDAAILPASENIENQQTSSPSKEYKNRESNKAASGKHM 960
            LQEDAYSLNPN +KSIEK NSG+ +    ASENI+NQQ  SPSKEYK+   N++ASG+HM
Sbjct: 901  LQEDAYSLNPNTKKSIEKLNSGIGSETPSASENIDNQQIPSPSKEYKDLGGNESASGQHM 960

Query: 961  LSYSTETTGEKKRGRPAARSLQKRTSGVEQVRRARARTTKGPAKISEVDSENSDHSDEK- 1020
            LS+ST+ +G KKRGRPA  S+QK  S +EQVRR   R TKGPAKI+EV+S++SDH++EK 
Sbjct: 961  LSHSTKRSGGKKRGRPAESSMQKGKSSIEQVRRVFPRRTKGPAKINEVESDSSDHTNEKT 1020

Query: 1021 ---IGNITTVRCENSTMNEFQTLENERMDEKKRGRPARNAPKRKAGVEPVRRTRARTAKR 1080
                GNI T+  E+   NEF+ LENER  +KKRG PARN  K KA VE VRRTRAR AK 
Sbjct: 1021 NAETGNIGTLCSESFGKNEFRMLENERPVKKKRGSPARNTQKGKARVEQVRRTRARIAKG 1080

Query: 1081 SASISNIFSDNSDSTNETGMEKGNIGTLWEENSKTDEFKTLEH-DSKQKGKAIEQNDLAE 1140
              S    ++D   +  E  ME+GN+GT+ ++NSKT+E K LE  D KQKGKAIEQ+D+A+
Sbjct: 1081 DGS---DYTDVKKNAEEAEMERGNVGTVCKDNSKTEELKMLEDCDWKQKGKAIEQSDVAD 1140

Query: 1141 PKSSAHEVDISERYKYKVEKSEKLEMMTDPLQAMLLDMVPSLGMSETK---SMSTVLEEK 1200
            PKS               + SEKLE+MTDPLQAMLLDMVPSLGMS+TK   S S+VLEEK
Sbjct: 1141 PKS---------------KNSEKLEVMTDPLQAMLLDMVPSLGMSQTKSSSSSSSVLEEK 1200

Query: 1201 QPIHNNSQPAAKKKVSYKDVASELLKDW 1214
            QP+HNNS+P AKKKVSYKDVASELLKDW
Sbjct: 1201 QPVHNNSEP-AKKKVSYKDVASELLKDW 1209

BLAST of Sed0026596 vs. ExPASy TrEMBL
Match: A0A6J1ENX2 (DNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111436208 PE=3 SV=1)

HSP 1 Score: 1956.0 bits (5066), Expect = 0.0e+00
Identity = 1012/1228 (82.41%), Postives = 1089/1228 (88.68%), Query Frame = 0

Query: 1    MTESMKFSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSPSDYFSAIRLILPSLDRERRT 60
            MTE +KFSVLSSLFDWIQRSKSS KKRSKFRKF+DTFCSP+DYFSAIRLILPSLDRER T
Sbjct: 1    MTELIKFSVLSSLFDWIQRSKSSAKKRSKFRKFIDTFCSPADYFSAIRLILPSLDRERGT 60

Query: 61   YGLKESVLATSLIDALGMSRDSDDALRLINWRKAGARAGANAGNFALLAAEVLQRRQGMT 120
            YGLKESVLAT LIDALGMSR+S+DALRLINWRK GA+ GAN GNFAL+AAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGMSRESEDALRLINWRKGGAKTGANVGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIKELNDLLDRLALSENRAEKTVVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180
            PGGL IKELNDLLDRLALSENRAEKT VLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS
Sbjct: 121  PGGLLIKELNDLLDRLALSENRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180

Query: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240
            IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK
Sbjct: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240

Query: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLSTRCILD 300
            KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVL+TRCILD
Sbjct: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLATRCILD 300

Query: 301  GEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDVLYVGDTSLIHRSLKERR 360
            GEMLVWDKSLNRFAEFGSNQEIA+AAKDGFDSNRQLCYVAFDVLYVGDTS+IHRSLKER 
Sbjct: 301  GEMLVWDKSLNRFAEFGSNQEIARAAKDGFDSNRQLCYVAFDVLYVGDTSVIHRSLKERH 360

Query: 361  ELLRNVVKPVKGRLEFLVPNGGLNNDCAGDPSWSMRAYNVDDVERFFKNTIENRDEGIVI 420
            ELLR+VVKPVKG+LE LVPNGGLN+DCAGDPSWS+RAYNVDDVERFFK+TIENRDEGIVI
Sbjct: 361  ELLRDVVKPVKGQLEILVPNGGLNSDCAGDPSWSIRAYNVDDVERFFKSTIENRDEGIVI 420

Query: 421  KDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLADRPH 480
            KDL SKWEPGDRSGKWLKLKPDY+ AGSDLDALIIGGYYGSGRRGGEVAQFLMGLA+RP 
Sbjct: 421  KDLSSKWEPGDRSGKWLKLKPDYIHAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERPP 480

Query: 481  SNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKKTAPSFYQVTNNSKERPDV 540
            SNA+PRRFISFCRVGTGLTDEELD+VVNKLKPYFRKSEYP+KTAPSFYQVTNNSKERPDV
Sbjct: 481  SNAYPRRFISFCRVGTGLTDEELDSVVNKLKPYFRKSEYPRKTAPSFYQVTNNSKERPDV 540

Query: 541  WIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHS 600
            WIESPEKSIILSITSDIRTI SEVFAAPYSLRFPRI RVRYDKPWHECLDVQSFVELVHS
Sbjct: 541  WIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIHRVRYDKPWHECLDVQSFVELVHS 600

Query: 601  SNGTTQ-------GQDSKQKYIKSTGKGRKKNISAVPSHLLQTDISGIKEDSLIFSNMLF 660
            SNGTTQ        QDSK+KYI STGKGRKK++S VPSHLLQTDISGIK DSLIFSNMLF
Sbjct: 601  SNGTTQRGTNNSGWQDSKRKYINSTGKGRKKSVSVVPSHLLQTDISGIKGDSLIFSNMLF 660

Query: 661  YIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNIAADSKGIKYEAAKRHGDVIHYSW 720
            YIVNVPPT+SLDSLHKLIVENGGTFSMNLN+SV H++AADSKGIKYEAAKRHGDVIHYSW
Sbjct: 661  YIVNVPPTHSLDSLHKLIVENGGTFSMNLNNSVTHSVAADSKGIKYEAAKRHGDVIHYSW 720

Query: 721  VLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLLNNVSRSQ 780
            VLECCSQKKLLPL PK+FLFLS HSKKKLEEEIDEFSDSYFWDLELADLKQLL+NVSRS+
Sbjct: 721  VLECCSQKKLLPLMPKHFLFLSGHSKKKLEEEIDEFSDSYFWDLELADLKQLLSNVSRSE 780

Query: 781  DVKAIDYYRGKYCPKHEWCIFVGCQIYFLPLRPSLKSDWDVLFELLIRRLKVEVSFRGGK 840
            DVKAIDYYR KYCPKHEWCIF GCQIYF PL+PSLKSDWD L EL +RRLKVEVSFRGGK
Sbjct: 781  DVKAIDYYREKYCPKHEWCIFSGCQIYFHPLKPSLKSDWDALLELSVRRLKVEVSFRGGK 840

Query: 841  VSDNHVSATHIVVFSMPGSPMGYETVLKRCSEAEKHVLLRDKIHIVAHQWLEDCLEMAHR 900
            VSD+ VSATHIVVF +PG P+ YE VLK C+EA+KHV+L+DK HI+ HQWLEDCLE A R
Sbjct: 841  VSDDRVSATHIVVFCIPGPPVKYEAVLKSCNEADKHVMLKDKTHIIGHQWLEDCLERAQR 900

Query: 901  LQEDAYSLNPNREKSIEKSNSGVDAAILPASENIENQQTSSPSKEYKNRESNKAASGKHM 960
            LQEDAYSLNPN +KSIEK NSG+ +    ASENI+NQQ  SPSKEYK+   N++ASG+HM
Sbjct: 901  LQEDAYSLNPNTKKSIEKLNSGIGSETPSASENIDNQQIPSPSKEYKDLGGNESASGQHM 960

Query: 961  LSYSTETTGEKKRGRPAARSLQKRTSGVEQVRRARARTTKGPAKISEVDSENSDHSDEK- 1020
            LS+ST+ +G KKRGRPAA S+QK  S +EQVRR   R TKGPAKI+EV+S++SDH++EK 
Sbjct: 961  LSHSTKGSGGKKRGRPAASSMQKGKSSIEQVRRVLPRRTKGPAKINEVESDSSDHTNEKT 1020

Query: 1021 ---IGNITTVRCENSTMNEFQTLENERMDEKKRGRPARNAPKRKAGVEPVRRTRARTAKR 1080
                GNI T+  E+   NEF+ LENER  +KKRG PA+N  K KA VE VRRTRAR AK 
Sbjct: 1021 NAETGNIGTLCSESLGKNEFRMLENERPVKKKRGSPAKNTQKGKARVEQVRRTRARIAKG 1080

Query: 1081 SASISNIFSDNSDSTNETGMEKGNIGTLWEENSKTDEFKTLEH-DSKQKGKAIEQNDLAE 1140
              S    ++D   +  E  ME+GN+GT+ ++NSKT+E K LE  D KQKGKAIEQ D+A+
Sbjct: 1081 DGS---DYTDVKTNAEEAEMERGNVGTVCKDNSKTEELKMLEDCDWKQKGKAIEQIDVAD 1140

Query: 1141 PKSSAHEVDISERYKYKVEKSEKLEMMTDPLQAMLLDMVPSLGMSETK---SMSTVLEEK 1200
             KS               E SEKLE+MTDPLQAMLLDMVPSLGMSETK   S S+VLEEK
Sbjct: 1141 TKSK------------NSENSEKLEVMTDPLQAMLLDMVPSLGMSETKSSSSSSSVLEEK 1200

Query: 1201 QPIHNNSQPAAKKKVSYKDVASELLKDW 1214
            QP        AKKKVSYKDVASELLKDW
Sbjct: 1201 QP--------AKKKVSYKDVASELLKDW 1205

BLAST of Sed0026596 vs. ExPASy TrEMBL
Match: A0A6J1D6X8 (DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1)

HSP 1 Score: 1941.8 bits (5029), Expect = 0.0e+00
Identity = 1015/1256 (80.81%), Postives = 1099/1256 (87.50%), Query Frame = 0

Query: 1    MTESMKFSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSPSDYFSAIRLILPSLDRERRT 60
            MTE + F+VLS+LFDWIQRSKSS KKRSKFRKFLDTFCS +DYFSAIRLILP+LDRER T
Sbjct: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60

Query: 61   YGLKESVLATSLIDALGMSRDSDDALRLINWRKAGARAGANAGNFALLAAEVLQRRQGMT 120
            YGLKESVLAT LIDALG+SRDSDDALRL NWRK GA+ GANAGNFAL+AAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGISRDSDDALRLNNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIKELNDLLDRLALSENRAEKTVVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180
            PGGLTIKELNDLLDRLALSENRAEKT VLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS
Sbjct: 121  PGGLTIKELNDLLDRLALSENRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180

Query: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240
            IFHEFHPDAEDLFNVTCDLKLVCEKLRDRR+RHKRQDIEVGKAVRPQLASRVSNANAAWK
Sbjct: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAWK 240

Query: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLSTRCILD 300
            KLHGKEVVVECKFDGDRIQIHKNGTEIH+FSRNFLDHSEYG GMSDVIV+N+++TRCILD
Sbjct: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENIIATRCILD 300

Query: 301  GEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDVLYVGDTSLIHRSLKERR 360
            GEMLVWDKSLNRFAEFGSNQEIA+AAKDG DS+RQLCYVAFDVLYVGDTS+IHRSLKER 
Sbjct: 301  GEMLVWDKSLNRFAEFGSNQEIARAAKDGLDSDRQLCYVAFDVLYVGDTSVIHRSLKERH 360

Query: 361  ELLRNVVKPVKGRLEFLVPNGGLNNDCAGDPSWSMRAYNVDDVERFFKNTIENRDEGIVI 420
            ELLR+VVKPVKGRLE LVPNGGLN+DCAGDPSWS RAYN DDVERFFKNTIENRDEGIVI
Sbjct: 361  ELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIVI 420

Query: 421  KDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLADRPH 480
            KD+GSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLA+R  
Sbjct: 421  KDMGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERSP 480

Query: 481  SNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKKTAPSFYQVTNNSKERPDV 540
            SNA+PRRFISFCRVGTGLTDE+LDAV NKL+PYFRKSEYPKK APSFYQVTNNSKERPDV
Sbjct: 481  SNAYPRRFISFCRVGTGLTDEDLDAVTNKLRPYFRKSEYPKKAAPSFYQVTNNSKERPDV 540

Query: 541  WIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHS 600
            WIESPEKSIILSITSDIRTI SEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHS
Sbjct: 541  WIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHS 600

Query: 601  SNGTTQ-------GQDSKQKYIKSTGKGRKKNISAVPSHLLQTDISGIKEDSLIFSNMLF 660
            SNG TQ        QDS +K+IKSTGK RKK++S VPSHLLQTDISGIKEDSLIFSNMLF
Sbjct: 601  SNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNMLF 660

Query: 661  YIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNIAADSKGIKYEAAKRHGDVIHYSW 720
            YIVNVPPT+SLDSLH+LIVENGGTFSMNLN SV H++AAD+KGIKYEAA+R GDVIHYSW
Sbjct: 661  YIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKGIKYEAARRRGDVIHYSW 720

Query: 721  VLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLLNNVSRSQ 780
            +LECCSQKKLLPLQPK+FLFLSDHSKKKLEEEID FSDSYFWDLELADLKQLL+NVSRS+
Sbjct: 721  MLECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSRSE 780

Query: 781  DVKAIDYYRGKYCPKHEWCIFVGCQIYFLPLRPSLKSDWDVLFELLIRRLKVEVSFRGGK 840
            DVKAIDYYRGKYCPK EW IFVG QIYFLPLRPSLK DWDVL EL +RRLKVEVSFRGGK
Sbjct: 781  DVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRGGK 840

Query: 841  VSDNHVSATHIVVFSMPGSPMGYETVLKRCSEAEKHVLLRDKIHIVAHQWLEDCLEMAHR 900
            VSDNHVSATH+VVF +PGS +  ETVLK  +EAEKHV+L++KIHIVAHQWLEDCLE AHR
Sbjct: 841  VSDNHVSATHVVVFCIPGSSVDCETVLKCFNEAEKHVILKNKIHIVAHQWLEDCLERAHR 900

Query: 901  LQEDAYSLNPNREKSIEKSNSGVDAAILPASENIENQQTSSPSKEYKNRESNKAASGKHM 960
            LQEDAYSL PN +KSIEK NS +D+    ASEN ENQ+ SSPS  YK++  N+AASG+HM
Sbjct: 901  LQEDAYSLKPNWKKSIEKLNSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQHM 960

Query: 961  LSYSTETTGEKKRGRPAARSLQKRTSGVEQVRRARARTTKGPAKISEVDSENSDHSDEKI 1020
            L +STE +G KKRG+  ARS+QK    VEQVRRARART KGPAKISEV+S++SD++DEK 
Sbjct: 961  LLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDEKT 1020

Query: 1021 G----NITTVRCENSTMNEFQTLEN------------------ERMDEKKRGR------P 1080
                 NI T   EN   NE   LEN                  ER  EKKRGR       
Sbjct: 1021 NAETENIGTPCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPTAS 1080

Query: 1081 ARNAPKRKAGVEPVRRTRARTAKRSASISNIFSDNSDSTNE------TGMEKGNIGTLWE 1140
            AR+  K KAGVE  +RTRARTAK SA I  + SD+SD+T++       G+EK NIGT+  
Sbjct: 1081 ARSMQKGKAGVEQGQRTRARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTVCP 1140

Query: 1141 ENSKTDEFKTLEH-DSKQKGKAIEQNDLAEPKSSAHEVDISERYKYK-VEKSEKLEMMTD 1200
             NS T+EF   E+ DS  KGK IEQ+DLAE KS+  E++IS RY  + +E+SEKLE+MTD
Sbjct: 1141 ANSNTNEFIWPENCDSSHKGKVIEQDDLAEFKSNVPEIEISTRYNCQGIEESEKLEVMTD 1200

Query: 1201 PLQAMLLDMVPSLGMSETKSMSTVLEEKQPIHNNSQPAAKKKVSYKDVASELLKDW 1214
            PLQAMLLDMVPSLGMSETKS STVLEEKQP+ NNS+P  KKKVSYKDVASELLKDW
Sbjct: 1201 PLQAMLLDMVPSLGMSETKSSSTVLEEKQPVQNNSEP-TKKKVSYKDVASELLKDW 1255

BLAST of Sed0026596 vs. ExPASy TrEMBL
Match: A0A6J1D7W8 (DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1)

HSP 1 Score: 1937.2 bits (5017), Expect = 0.0e+00
Identity = 1015/1257 (80.75%), Postives = 1099/1257 (87.43%), Query Frame = 0

Query: 1    MTESMKFSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSPSDYFSAIRLILPSLDRERRT 60
            MTE + F+VLS+LFDWIQRSKSS KKRSKFRKFLDTFCS +DYFSAIRLILP+LDRER T
Sbjct: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60

Query: 61   YGLKESVLATSLIDALGMSRDSDDALRLINWRKAGARAGANAGNFALLAAEVLQRRQGMT 120
            YGLKESVLAT LIDALG+SRDSDDALRL NWRK GA+ GANAGNFAL+AAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGISRDSDDALRLNNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIKELNDLLDRLALSEN-RAEKTVVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180
            PGGLTIKELNDLLDRLALSEN RAEKT VLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK
Sbjct: 121  PGGLTIKELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180

Query: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAW 240
            SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRR+RHKRQDIEVGKAVRPQLASRVSNANAAW
Sbjct: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240

Query: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLSTRCIL 300
            KKLHGKEVVVECKFDGDRIQIHKNGTEIH+FSRNFLDHSEYG GMSDVIV+N+++TRCIL
Sbjct: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENIIATRCIL 300

Query: 301  DGEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDVLYVGDTSLIHRSLKER 360
            DGEMLVWDKSLNRFAEFGSNQEIA+AAKDG DS+RQLCYVAFDVLYVGDTS+IHRSLKER
Sbjct: 301  DGEMLVWDKSLNRFAEFGSNQEIARAAKDGLDSDRQLCYVAFDVLYVGDTSVIHRSLKER 360

Query: 361  RELLRNVVKPVKGRLEFLVPNGGLNNDCAGDPSWSMRAYNVDDVERFFKNTIENRDEGIV 420
             ELLR+VVKPVKGRLE LVPNGGLN+DCAGDPSWS RAYN DDVERFFKNTIENRDEGIV
Sbjct: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420

Query: 421  IKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLADRP 480
            IKD+GSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLA+R 
Sbjct: 421  IKDMGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480

Query: 481  HSNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKKTAPSFYQVTNNSKERPD 540
             SNA+PRRFISFCRVGTGLTDE+LDAV NKL+PYFRKSEYPKK APSFYQVTNNSKERPD
Sbjct: 481  PSNAYPRRFISFCRVGTGLTDEDLDAVTNKLRPYFRKSEYPKKAAPSFYQVTNNSKERPD 540

Query: 541  VWIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600
            VWIESPEKSIILSITSDIRTI SEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH
Sbjct: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600

Query: 601  SSNGTTQ-------GQDSKQKYIKSTGKGRKKNISAVPSHLLQTDISGIKEDSLIFSNML 660
            SSNG TQ        QDS +K+IKSTGK RKK++S VPSHLLQTDISGIKEDSLIFSNML
Sbjct: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660

Query: 661  FYIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNIAADSKGIKYEAAKRHGDVIHYS 720
            FYIVNVPPT+SLDSLH+LIVENGGTFSMNLN SV H++AAD+KGIKYEAA+R GDVIHYS
Sbjct: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKGIKYEAARRRGDVIHYS 720

Query: 721  WVLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLLNNVSRS 780
            W+LECCSQKKLLPLQPK+FLFLSDHSKKKLEEEID FSDSYFWDLELADLKQLL+NVSRS
Sbjct: 721  WMLECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSRS 780

Query: 781  QDVKAIDYYRGKYCPKHEWCIFVGCQIYFLPLRPSLKSDWDVLFELLIRRLKVEVSFRGG 840
            +DVKAIDYYRGKYCPK EW IFVG QIYFLPLRPSLK DWDVL EL +RRLKVEVSFRGG
Sbjct: 781  EDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRGG 840

Query: 841  KVSDNHVSATHIVVFSMPGSPMGYETVLKRCSEAEKHVLLRDKIHIVAHQWLEDCLEMAH 900
            KVSDNHVSATH+VVF +PGS +  ETVLK  +EAEKHV+L++KIHIVAHQWLEDCLE AH
Sbjct: 841  KVSDNHVSATHVVVFCIPGSSVDCETVLKCFNEAEKHVILKNKIHIVAHQWLEDCLERAH 900

Query: 901  RLQEDAYSLNPNREKSIEKSNSGVDAAILPASENIENQQTSSPSKEYKNRESNKAASGKH 960
            RLQEDAYSL PN +KSIEK NS +D+    ASEN ENQ+ SSPS  YK++  N+AASG+H
Sbjct: 901  RLQEDAYSLKPNWKKSIEKLNSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQH 960

Query: 961  MLSYSTETTGEKKRGRPAARSLQKRTSGVEQVRRARARTTKGPAKISEVDSENSDHSDEK 1020
            ML +STE +G KKRG+  ARS+QK    VEQVRRARART KGPAKISEV+S++SD++DEK
Sbjct: 961  MLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDEK 1020

Query: 1021 IG----NITTVRCENSTMNEFQTLEN------------------ERMDEKKRGR------ 1080
                  NI T   EN   NE   LEN                  ER  EKKRGR      
Sbjct: 1021 TNAETENIGTPCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPTA 1080

Query: 1081 PARNAPKRKAGVEPVRRTRARTAKRSASISNIFSDNSDSTNE------TGMEKGNIGTLW 1140
             AR+  K KAGVE  +RTRARTAK SA I  + SD+SD+T++       G+EK NIGT+ 
Sbjct: 1081 SARSMQKGKAGVEQGQRTRARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTVC 1140

Query: 1141 EENSKTDEFKTLEH-DSKQKGKAIEQNDLAEPKSSAHEVDISERYKYK-VEKSEKLEMMT 1200
              NS T+EF   E+ DS  KGK IEQ+DLAE KS+  E++IS RY  + +E+SEKLE+MT
Sbjct: 1141 PANSNTNEFIWPENCDSSHKGKVIEQDDLAEFKSNVPEIEISTRYNCQGIEESEKLEVMT 1200

Query: 1201 DPLQAMLLDMVPSLGMSETKSMSTVLEEKQPIHNNSQPAAKKKVSYKDVASELLKDW 1214
            DPLQAMLLDMVPSLGMSETKS STVLEEKQP+ NNS+P  KKKVSYKDVASELLKDW
Sbjct: 1201 DPLQAMLLDMVPSLGMSETKSSSTVLEEKQPVQNNSEP-TKKKVSYKDVASELLKDW 1256

BLAST of Sed0026596 vs. ExPASy TrEMBL
Match: A0A6J1D8A6 (DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1)

HSP 1 Score: 1937.2 bits (5017), Expect = 0.0e+00
Identity = 1015/1257 (80.75%), Postives = 1099/1257 (87.43%), Query Frame = 0

Query: 1    MTESMKFSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSPSDYFSAIRLILPSLDRERRT 60
            MTE + F+VLS+LFDWIQRSKSS KKRSKFRKFLDTFCS +DYFSAIRLILP+LDRER T
Sbjct: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60

Query: 61   YGLKESVLATSLIDALGMSRDSDDALRLINWRKAGARAGANAGNFALLAAEVLQRRQGMT 120
            YGLKESVLAT LIDALG+SRDSDDALRL NWRK GA+ GANAGNFAL+AAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGISRDSDDALRLNNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIKELNDLLDRLALSENRAEKTVVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180
            PGGLTIKELNDLLDRLALSENRAEKT VLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS
Sbjct: 121  PGGLTIKELNDLLDRLALSENRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEKS 180

Query: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAWK 240
            IFHEFHPDAEDLFNVTCDLKLVCEKLRDRR+RHKRQDIEVGKAVRPQLASRVSNANAAWK
Sbjct: 181  IFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAWK 240

Query: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLSTRCILD 300
            KLHGKEVVVECKFDGDRIQIHKNGTEIH+FSRNFLDHSEYG GMSDVIV+N+++TRCILD
Sbjct: 241  KLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENIIATRCILD 300

Query: 301  GEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDVLYVGDTSLIHRSLKERR 360
            GEMLVWDKSLNRFAEFGSNQEIA+AAKDG DS+RQLCYVAFDVLYVGDTS+IHRSLKER 
Sbjct: 301  GEMLVWDKSLNRFAEFGSNQEIARAAKDGLDSDRQLCYVAFDVLYVGDTSVIHRSLKERH 360

Query: 361  ELLRNVVKPVKGRLEFLVPNGGLNNDCAGDPSWSMRAYNVDDVERFFKNTIENRDEGIVI 420
            ELLR+VVKPVKGRLE LVPNGGLN+DCAGDPSWS RAYN DDVERFFKNTIENRDEGIVI
Sbjct: 361  ELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIVI 420

Query: 421  KDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLADRPH 480
            KD+GSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLA+R  
Sbjct: 421  KDMGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERSP 480

Query: 481  SNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKKTAPSFYQVTNNSKERPDV 540
            SNA+PRRFISFCRVGTGLTDE+LDAV NKL+PYFRKSEYPKK APSFYQVTNNSKERPDV
Sbjct: 481  SNAYPRRFISFCRVGTGLTDEDLDAVTNKLRPYFRKSEYPKKAAPSFYQVTNNSKERPDV 540

Query: 541  WIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHS 600
            WIESPEKSIILSITSDIRTI SEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHS
Sbjct: 541  WIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHS 600

Query: 601  SNGTTQ-------GQDSKQKYIKSTGKGRKKNISAVPSHLLQTDISGIKEDSLIFSNMLF 660
            SNG TQ        QDS +K+IKSTGK RKK++S VPSHLLQTDISGIKEDSLIFSNMLF
Sbjct: 601  SNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNMLF 660

Query: 661  YIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNIAADSK-GIKYEAAKRHGDVIHYS 720
            YIVNVPPT+SLDSLH+LIVENGGTFSMNLN SV H++AAD+K GIKYEAA+R GDVIHYS
Sbjct: 661  YIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAARRRGDVIHYS 720

Query: 721  WVLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLLNNVSRS 780
            W+LECCSQKKLLPLQPK+FLFLSDHSKKKLEEEID FSDSYFWDLELADLKQLL+NVSRS
Sbjct: 721  WMLECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSRS 780

Query: 781  QDVKAIDYYRGKYCPKHEWCIFVGCQIYFLPLRPSLKSDWDVLFELLIRRLKVEVSFRGG 840
            +DVKAIDYYRGKYCPK EW IFVG QIYFLPLRPSLK DWDVL EL +RRLKVEVSFRGG
Sbjct: 781  EDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRGG 840

Query: 841  KVSDNHVSATHIVVFSMPGSPMGYETVLKRCSEAEKHVLLRDKIHIVAHQWLEDCLEMAH 900
            KVSDNHVSATH+VVF +PGS +  ETVLK  +EAEKHV+L++KIHIVAHQWLEDCLE AH
Sbjct: 841  KVSDNHVSATHVVVFCIPGSSVDCETVLKCFNEAEKHVILKNKIHIVAHQWLEDCLERAH 900

Query: 901  RLQEDAYSLNPNREKSIEKSNSGVDAAILPASENIENQQTSSPSKEYKNRESNKAASGKH 960
            RLQEDAYSL PN +KSIEK NS +D+    ASEN ENQ+ SSPS  YK++  N+AASG+H
Sbjct: 901  RLQEDAYSLKPNWKKSIEKLNSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQH 960

Query: 961  MLSYSTETTGEKKRGRPAARSLQKRTSGVEQVRRARARTTKGPAKISEVDSENSDHSDEK 1020
            ML +STE +G KKRG+  ARS+QK    VEQVRRARART KGPAKISEV+S++SD++DEK
Sbjct: 961  MLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDEK 1020

Query: 1021 IG----NITTVRCENSTMNEFQTLEN------------------ERMDEKKRGR------ 1080
                  NI T   EN   NE   LEN                  ER  EKKRGR      
Sbjct: 1021 TNAETENIGTPCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPTA 1080

Query: 1081 PARNAPKRKAGVEPVRRTRARTAKRSASISNIFSDNSDSTNE------TGMEKGNIGTLW 1140
             AR+  K KAGVE  +RTRARTAK SA I  + SD+SD+T++       G+EK NIGT+ 
Sbjct: 1081 SARSMQKGKAGVEQGQRTRARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTVC 1140

Query: 1141 EENSKTDEFKTLEH-DSKQKGKAIEQNDLAEPKSSAHEVDISERYKYK-VEKSEKLEMMT 1200
              NS T+EF   E+ DS  KGK IEQ+DLAE KS+  E++IS RY  + +E+SEKLE+MT
Sbjct: 1141 PANSNTNEFIWPENCDSSHKGKVIEQDDLAEFKSNVPEIEISTRYNCQGIEESEKLEVMT 1200

Query: 1201 DPLQAMLLDMVPSLGMSETKSMSTVLEEKQPIHNNSQPAAKKKVSYKDVASELLKDW 1214
            DPLQAMLLDMVPSLGMSETKS STVLEEKQP+ NNS+P  KKKVSYKDVASELLKDW
Sbjct: 1201 DPLQAMLLDMVPSLGMSETKSSSTVLEEKQPVQNNSEP-TKKKVSYKDVASELLKDW 1256

BLAST of Sed0026596 vs. TAIR 10
Match: AT5G57160.1 (DNA ligase IV )

HSP 1 Score: 1387.9 bits (3591), Expect = 0.0e+00
Identity = 740/1257 (58.87%), Postives = 918/1257 (73.03%), Query Frame = 0

Query: 1    MTESMKFSVLSSLFDWIQRSKSSGKKRSKFRKFLDTFCSPSDYFSAIRLILPSLDRERRT 60
            MTE +KFSVL SLF+WIQ+SK+S +KRSKFRKFLDT+C PSDYF A+RLI+PSLDRER +
Sbjct: 1    MTEEIKFSVLVSLFNWIQKSKTSSQKRSKFRKFLDTYCKPSDYFVAVRLIIPSLDRERGS 60

Query: 61   YGLKESVLATSLIDALGMSRDSDDALRLINWRKAG-ARAGANAGNFALLAAEVLQRRQGM 120
            YGLKESVLAT LIDALG+SRD+ DA+RL+NWRK G A+AGANAGNF+L+AAEVLQRRQGM
Sbjct: 61   YGLKESVLATCLIDALGISRDAPDAVRLLNWRKGGTAKAGANAGNFSLIAAEVLQRRQGM 120

Query: 121  TPGGLTIKELNDLLDRLALSENRAEKTVVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180
              GGLTIKELNDLLDRLA SENRAEKT+VLSTLIQKTNAQEMKW++ IILKDLKLG+SEK
Sbjct: 121  ASGGLTIKELNDLLDRLASSENRAEKTLVLSTLIQKTNAQEMKWVIRIILKDLKLGMSEK 180

Query: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEVGKAVRPQLASRVSNANAAW 240
            SIF EFHPDAEDLFNVTCDLKLVCEKLRDR QRHKRQDIEVGKAVRPQLA R+ + NAAW
Sbjct: 181  SIFQEFHPDAEDLFNVTCDLKLVCEKLRDRHQRHKRQDIEVGKAVRPQLAMRIGDVNAAW 240

Query: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHYFSRNFLDHSEYGHGMSDVIVQNVLSTRCIL 300
            KKLHGK+VV ECKFDGDRIQIHKNGT+IHYFSRNFLDHSEY H MSD+IVQN+L  +CIL
Sbjct: 241  KKLHGKDVVAECKFDGDRIQIHKNGTDIHYFSRNFLDHSEYAHAMSDLIVQNILVDKCIL 300

Query: 301  DGEMLVWDKSLNRFAEFGSNQEIAKAAKDGFDSNRQLCYVAFDVLYVGDTSLIHRSLKER 360
            DGEMLVWD SLNRFAEFGSNQEIAKAA++G DS++QLCYVAFDVLYVGDTS+IH+SLKER
Sbjct: 301  DGEMLVWDTSLNRFAEFGSNQEIAKAAREGLDSHKQLCYVAFDVLYVGDTSVIHQSLKER 360

Query: 361  RELLRNVVKPVKGRLEFLVPNGGLN-NDCAGDPSWSMRAYNVDDVERFFKNTIENRDEGI 420
             ELL+ VVKP+KGRLE LVP GGLN +  +G+PSWS+  +   DVERFFK T+ENRDEGI
Sbjct: 361  HELLKKVVKPLKGRLEVLVPEGGLNVHRPSGEPSWSIVVHAAADVERFFKETVENRDEGI 420

Query: 421  VIKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLADR 480
            V+KDL SKWEPGDRSGKW+KLKP+Y+RAG+DLD LIIGGYYGSGRRGGEVAQFL+ LADR
Sbjct: 421  VLKDLESKWEPGDRSGKWMKLKPEYIRAGADLDVLIIGGYYGSGRRGGEVAQFLVALADR 480

Query: 481  PHSNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKKTAPSFYQVTNNSKERP 540
              +N +PRRF+SFCRVGTGL+D+EL+ VV+KLKPYFRK+E+PKK  PSFYQVTN+SKERP
Sbjct: 481  AEANVYPRRFMSFCRVGTGLSDDELNTVVSKLKPYFRKNEHPKKAPPSFYQVTNHSKERP 540

Query: 541  DVWIESPEKSIILSITSDIRTINSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELV 600
            DVWI+SPEKSIILSITSDIRTI SEVF APYSLRFPRID+VRYDKPWHECLDVQ+FVELV
Sbjct: 541  DVWIDSPEKSIILSITSDIRTIRSEVFVAPYSLRFPRIDKVRYDKPWHECLDVQAFVELV 600

Query: 601  HSSNGTTQGQDSKQ------KYIKSTGKGRKKNISAVPSHLLQTDISGIKEDSLIFSNML 660
            +SSNGTTQ Q   +      K  KS+ +G KKN+S VPS  +QTD+S IK  + IFSNM+
Sbjct: 601  NSSNGTTQKQKESESTQDNPKVNKSSKRGEKKNVSLVPSQFIQTDVSDIKGKTSIFSNMI 660

Query: 661  FYIVNVPPTYSLDSLHKLIVENGGTFSMNLNSSVKHNIAADSKGIKYEAAKRHGDVIHYS 720
            FY VNVP ++SL++ HK++VENGG FSMNLN+SV H IAA+S GIKY+AAKR  DVIH+S
Sbjct: 661  FYFVNVPRSHSLETFHKMVVENGGKFSMNLNNSVTHCIAAESSGIKYQAAKRQRDVIHFS 720

Query: 721  WVLECCSQKKLLPLQPKYFLFLSDHSKKKLEEEIDEFSDSYFWDLELADLKQLLNNVSRS 780
            WVL+CCS+ K+LPL PKYFL L+D S+ KL+++IDEFSDSY+WDL+L  LKQ+L+N  +S
Sbjct: 721  WVLDCCSRNKMLPLLPKYFLHLTDASRTKLQDDIDEFSDSYYWDLDLEGLKQVLSNAKQS 780

Query: 781  QDVKAIDYYRGKYCPKHEWCIFVGCQIYFLPLRPSLKSDWDVLFELLIRRLKVEVSFRGG 840
            +D K+IDYY+ K CP+  W   + C +YF P   +L ++ + L  ++ +RL +EV   GG
Sbjct: 781  EDSKSIDYYKKKLCPEKRWSCLLSCCVYFYPYSQTLSTEEEALLGIMAKRLMLEVLMAGG 840

Query: 841  KVSDNHVSATHIVVFSMPGSPMGYETVLKRCSEAEKHVLLRDKIHIVAHQWLEDCLEMAH 900
            KVS+N   A+H+VV +M   P+ +  V K  SE EK +LL+ ++H+V+  WLE+ L+   
Sbjct: 841  KVSNNLAHASHLVVLAMAEEPLDFTLVSKSFSEMEKRLLLKKRLHVVSSHWLEESLQREE 900

Query: 901  RLQEDAYSLNPNREKSIEKSNSGVDAAILPASENIENQQTSSPSKEYKNRESNKAASGKH 960
            +L ED Y+L P   K +E+S    D      SE+   +  S  S + K   S+K A    
Sbjct: 901  KLCEDVYTLRP---KYMEES----DTEESDKSEHDTTEVASQGSAQTKEPASSKIAI--- 960

Query: 961  MLSYSTETTGEKKRGRPAARSLQKRTSGVEQVRRARARTTKGPAKISEVDSENSDHSDEK 1020
                         RGR   R++++  S    ++R + R  K P+KIS  ++E SD S+EK
Sbjct: 961  ----------TSSRGRSNTRAVKRGRSSTNSLQRVQRRRGKQPSKISGDETEESDASEEK 1020

Query: 1021 IG-NITTVRCENSTMNEFQTLENERMDEKKRGRPARNAPKRKAGVEPVRRTRARTAKRSA 1080
            +   ++ +  E  +  E Q           RG+ A+    R    + V+  R+R  K++A
Sbjct: 1021 VSTRLSDIAEETDSFGEAQR-------NSSRGKCAKRGKSRVGQTQRVQ--RSRRGKKAA 1080

Query: 1081 SISNIFSDNSDSTN-----ETGMEKGNIGTLWEENSKTDEFKTLEH-DSKQKGKAIEQND 1140
             I    SD +D  +         E+GN      EN +T E    ++ +S+Q+   +   +
Sbjct: 1081 KIGGDESDENDELDGNNNVSADAEEGNAAGRSVENEETREPDIAKYTESQQRDNTVAVEE 1140

Query: 1141 LAEPKSSAHEVDISERYKYKVEKSEKLEMMTDPLQAMLLDM--VPSLGMSETKSMSTVLE 1200
              +   +A         K +++  EKL++  DPLQAML+ M  +PS   +ET + +T   
Sbjct: 1141 ALQDSRNA---------KTEMDMKEKLQIHEDPLQAMLMKMFPIPSQKTTETSNRTTGEY 1200

Query: 1201 EKQPIHNNSQPA---------------------------AKKKVSYKDVASELLKDW 1214
             K  +    + +                            KKKVSY+DVA ELLKDW
Sbjct: 1201 RKANVSGECESSEKRKLDAETDNTSVNAGAESDVVPPLVKKKKVSYRDVAGELLKDW 1219

BLAST of Sed0026596 vs. TAIR 10
Match: AT1G08130.1 (DNA ligase 1 )

HSP 1 Score: 131.0 bits (328), Expect = 6.7e-30
Identity = 113/397 (28.46%), Postives = 178/397 (44.84%), Query Frame = 0

Query: 214 KRQDIEVGKAVRPQLASRVSNANAAWKKLHGKEVVVECKFDGDRIQIH--KNGT-EIH-- 273
           K  +  +G  + P LA           K        E K+DG+R QIH  ++GT EI+  
Sbjct: 406 KTCNFTLGVPIGPMLAKPTKGVAEILNKFQDIVFTCEYKYDGERAQIHFMEDGTFEIYSR 465

Query: 274 YFSRNFLDHSEYGHGMSDVIVQNVLSTRCILDGEMLVWDKSLNRFAEFGSNQEIAKAAKD 333
              RN   + +    +S +   +V S   ILD E++ +D+   +   F      A+   +
Sbjct: 466 NAERNTGKYPDVALALSRLKKPSVKS--FILDCEVVAFDREKKKILPFQILSTRARKNVN 525

Query: 334 GFDSNRQLCYVAFDVLYVGDTSLIHRSLKERRELLRNVVKPVKGRLEFLVPNGGLNNDCA 393
             D    +C  AFD+LY+    LI  +LK RRE L    +   G  +F            
Sbjct: 526 VNDIKVGVCIFAFDMLYLNGQQLIQENLKIRREKLYESFEEDPGYFQF------------ 585

Query: 394 GDPSWSMRAYNVDDVERFFKNTIENRDEGIVIKDLGS--KWEPGDRSGKWLKLKPDYVRA 453
              + ++ + ++D++++F   +++   EG++IK L S   +EP  RS  WLKLK DY+ +
Sbjct: 586 ---ATAVTSNDIDEIQKFLDASVDVGCEGLIIKTLDSDATYEPAKRSNNWLKLKKDYMDS 645

Query: 454 -GSDLDALIIGGYYGSGRRGGEVAQFLMGLADRPHSNAHPRRFISFCRVGTGLTDEELDA 513
            G  +D + I  ++G G+R G    FL+   D          F S C++GTG +D  LD 
Sbjct: 646 IGDSVDLVPIAAFHGRGKRTGVYGAFLLACYD-----VDKEEFQSICKIGTGFSDAMLDE 705

Query: 514 VVNKLKPYFRKSEYPKKTAPSFYQVTNNSKERPDVWIESPE----KSIILSITSDIRTIN 573
             + L+           T   +Y+V ++    PDVW E  E    K+  L+I+   R   
Sbjct: 706 RSSSLR------SQVIATPKQYYRVGDSL--NPDVWFEPTEVWEVKAADLTISPVHRAAT 765

Query: 574 SEVFA-APYSLRFPRIDRVRYDKPWHECLDVQSFVEL 598
             V      SLRFPR+ RVR DK   E    +   +L
Sbjct: 766 GIVDPDKGISLRFPRLLRVREDKKPEEATSSEQIADL 772

BLAST of Sed0026596 vs. TAIR 10
Match: AT1G49250.1 (ATP-dependent DNA ligase )

HSP 1 Score: 129.8 bits (325), Expect = 1.5e-29
Identity = 114/416 (27.40%), Postives = 181/416 (43.51%), Query Frame = 0

Query: 214 KRQDIEVGKAVRPQLASRVSNANAAWKKLHGKEVVVECKFDGDRIQIH--KNGTEIHYFS 273
           K  ++ +G  VRP LA   +  +   +K        E K+DG+R QI+  ++GT +  FS
Sbjct: 275 KTCNLTLGVPVRPMLAKATTRVDLILEKFKDTVFTAEYKYDGERAQIYYMEDGT-VEIFS 334

Query: 274 RNFLDHSEYGHG-MSDVIVQNVLS-------TRCILDGEMLVWDKSLNRFAEFGSNQEIA 333
           R    H+E   G   DV +  VLS          ILD E++ +++   +     S +  A
Sbjct: 335 R----HAERNTGKYPDVAL--VLSRLKKPTVKSFILDCEVVTFNREKEKILPLQSTR--A 394

Query: 334 KAAKDGFDSNRQLCYVAFDVLYVGDTSLIHRSLKERRELLRNVVKPVKGRLEFLVPNGGL 393
               +  D    +C  AFD+LY+    LIH +L  RRE L +  +   G  +F       
Sbjct: 395 HKNVNVSDIKVGVCVFAFDILYLNGQLLIHENLNIRREKLHDSFEEDLGYFQF------- 454

Query: 394 NNDCAGDPSWSMRAYNVDDVERFFKNTIENRDEGIVIKDLGSK--WEPGDRSGKWLKLKP 453
                   + ++ + ++ +++ F K +I+   EG++IK L S   +EP  RS  WLKLK 
Sbjct: 455 --------ATALTSNDIGELQEFLKASIDIGCEGLMIKSLYSNATYEPAKRSNNWLKLKK 514

Query: 454 DYV-RAGSDLDALIIGGYYGSGRRGGEVAQFLMGLADRPHSNAHPRRFISFCRVGTGLTD 513
           DY+   G  +D + I  ++G G+R G    +L+   D          F S C++GT  +D
Sbjct: 515 DYMDNIGDSVDLVPIATFHGRGKRTGFFGAYLLACYD-----VDKEEFQSICKIGTEFSD 574

Query: 514 EELDAVVNKLKPYFRKSEYPKKTAPSFYQVTNNSKERPDVWIESPEKSIILSITSDIRTI 573
            EL  + + L            T   +YQV N+    PDVW+E  E   + +    +  +
Sbjct: 575 VELQDLSSSL------CSKVIATPKQYYQVDNDL--NPDVWLEPTEVWEVKAADLTVSPV 634

Query: 574 NSEVFA-----APYSLRFPRIDRVRYDKPWHECLDVQSFVELVHSSNGTTQGQDSK 612
             E           SLRFPR+ R+R DK   E        E+  +          K
Sbjct: 635 YREAIGIVDPDKGISLRFPRLVRIRKDKNPEEATTSDQIAEMYQAQKHNQPSNQGK 653

BLAST of Sed0026596 vs. TAIR 10
Match: AT1G66730.1 (DNA LIGASE 6 )

HSP 1 Score: 126.7 bits (317), Expect = 1.3e-28
Identity = 142/618 (22.98%), Postives = 263/618 (42.56%), Query Frame = 0

Query: 39   SPSDYFSAIRLILPSL--DRERRTYGLKESVLATSLIDALGMSRDSDDALRLINWRKAGA 98
            SP D   A+ L    +  D E     +  S+++++L +A G+SR +   +    +   G 
Sbjct: 801  SPEDVLPAVYLCTNKIAADHENIELNIGGSLISSALEEACGISRSTVRDM----YNSLG- 860

Query: 99   RAGANAGNFALLAAEVLQRRQGMTPGGLTIKELNDLLDRLAL----SENRAEKTVVLSTL 158
                + G+ A L  +   ++  + P  L ++++   L ++++       R +K +++  L
Sbjct: 861  ----DLGDVAQLCRQT--QKLLVPPPPLLVRDVFSTLRKISVQTGTGSTRLKKNLIVK-L 920

Query: 159  IQKTNAQEMKWIVMIILKDLKLGVSEKS---------IFHEFHPD--------------- 218
            ++    +E+K++V  + ++L++G   ++         + + F  D               
Sbjct: 921  MRSCREKEIKFLVRTLARNLRIGAMLRTVLPALGRAIVMNSFWNDHNKELSESCFREKLE 980

Query: 219  -----AEDLFNVTCDLKLVCEKLRDRRQRHKRQDIEV--GKAVRPQLASRVSNANAAWKK 278
                   + +N+   L +V   L D+        + +  G  ++P LA         +  
Sbjct: 981  GVSAAVVEAYNILPSLDVVVPSLMDKDIEFSTSTLSMVPGIPIKPMLAKIAKGVQEFFNL 1040

Query: 279  LHGKEVVVECKFDGDRIQIHK--NGTEIHYFSRNFLDHSEYGHGMSDVIVQ--NVLSTRC 338
               K    E K+DG R QIHK  +GT +  FSRN  + +     + DVI Q     +   
Sbjct: 1041 SQEKAFTCEYKYDGQRAQIHKLLDGT-VCIFSRNGDETTSRFPDLVDVIKQFSCPAAETF 1100

Query: 339  ILDGEMLVWDK----SLNRFAEFGSNQEIAKAAKDGFDSNR-QLCYVAFDVLYVGDTSLI 398
            +LD E++  D+     L  F E  + +  +K A    +S + ++C   FD+++V    L+
Sbjct: 1101 MLDAEVVATDRINGNKLMSFQELSTRERGSKDALITTESIKVEVCVFVFDIMFVNGEQLL 1160

Query: 399  HRSLKERRELLRNVVKPVK-GRLEFLVPNGGLNNDCAGDPSWSMRAYN-VDDVERFFKNT 458
               L+ERR  L+ V    + G LE+            G    S+  ++ +  +  F +  
Sbjct: 1161 ALPLRERRRRLKEVFPETRPGYLEY------AKEITVGAEEASLNNHDTLSRINAFLEEA 1220

Query: 459  IENRDEGIVIK--DLGSKWEPGDRSGKWLKLKPDYVRA-GSDLDALIIGGYYGSGRRGGE 518
             ++  EGI++K  D+ + + P  RS  WLK+K DYV   G  LD + IG +YG+GR+ G 
Sbjct: 1221 FQSSCEGIMVKSLDVNAGYCPTKRSDSWLKVKRDYVDGLGDTLDLVPIGAWYGNGRKAGW 1280

Query: 519  VAQFLMGLADRPHSNAHPRRFISFCRVGTGLTDEELDAVVNKLKPYFRKSEYPKKTAPSF 578
             + FLM        N     F S CRV +G +    DA   ++K ++ + +   K  P +
Sbjct: 1281 YSPFLMAC-----FNPETEEFQSVCRVMSGFS----DAFYIEMKEFYSEDKILAKKPPYY 1340

Query: 579  YQVTNNSKERPDVWIESPEKSIILSITSDIRTINSEVFAAPY-----SLRFPRIDRVRYD 601
                  + E PD+W  +     I      +  ++S      +     S+RFPR      D
Sbjct: 1341 -----RTGETPDMWFSAEVVWEIRGADFTVSPVHSASLGLVHPSRGISVRFPRFISKVTD 1385