Sed0026352 (gene) Chayote v1

Overview
NameSed0026352
Typegene
OrganismSechium edule (Chayote v1)
DescriptionDNA-directed RNA polymerase subunit alpha
LocationLG13: 18752861 .. 18757489 (+)
RNA-Seq ExpressionSed0026352
SyntenySed0026352
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGGAATGTCTTATCGAGGCAATCGTATTTGCTTCGGTAGATACGCTATTCAGGCACTTGAACCCGCTTGGATCACATCTAGACAAATCGAAGCGGGACGAAGGGCAATGACACGAAATGCACGACGCGGCGGAAAAATATGGGTACGTATATTTCCAGACAAACCCGTTACACTAAGACCCACAGAAACACGTATGGGTTCAGGGAAAGGATCCCCCGAATATTGGGTAGCTGTTATTAAACCAGGTAGAATACTTTATGAAATGGGTGGAGTAGCAGAAAATATAGCCAGAAAAGCTATTTCAATAGCGGCGTCCAAACTGCCTATACGAACCCAATTCATTACTTCGGCATAAAAATGTAGAATCAAAGGAAAGCAGTCTTTGTTTGAGATGAAAAAACAACAATGGCAGATTTCTTTTTTTTTTTTTGACAAACAATATTTCTTTTTTTTTATTTCGCCCTTTGCATTGAAAGAAAAGATTCCAAAATAATATGATTCAACCTCAAACCCTTTTGAATGTAGCAGATAACAGCGGAGCCCGAGAATTGATGTGTATTCGAATCATAGGGGCTAGTAATCGACGATATGCTCATATTGGTGACATTATTGTTGCTGTAATCAAGAAAGTTGGTCCCAAATACACCTCTAGAAAGATCAGAAGTGATCAGAGCTGTAATTGTACGTACTTGTAAAGAACTCAAACGAGAAAACGGTATGATAATACGATATGATGACAATGCTGCGGTTGTTATTGATCAAGAAGGAAATCCAAAAGGAACTCGAATTTTTGGTGCGATTGCCCGAGAATTGAGACAGTTAAATTTCACTAAAATAATTTCATTAGCACCTGAAGTATTATAAGATGGGACCCTGAAATAGTTATAGTATTGGAATAAAATTAATTAGTAGATTGTGTATCACGTATATACCTTTTAAAATTTAAAACGATTTAATAAAAAAGCATGTTGATTAGATCCAAATTCAAAGTAACCAAAAATTTTCGTTTATCATGGGTAAGGACACTATTGCTAACATAATAACCTCTATTCGCAATGCTGACATGAATAGAAAAGGAATGGTTCGAATACCATCTACTAACATCAACGAAAACATTGTTAAAATACTTTTACGAGAAGGTTTTATTGAAAACGTAAGGAAACATCGGGAAAGCAACAAGGATTTTTGGGTTTTAACCCTACGACATAGAAGAAATAGGAAAGGACCATATAAAACGATTTTTAATTTAAACGGATCAGTCGACCTGGTCTCCGAATCTATTCTAACTATCAACGAATTCCTAGAATTTTAGGTGGAATGGGCATTGTAATTCTTTCTACTTCGGGGTATAATGACAGACCGAGAAGCTCGACTACAGGAATCGGCGGAGAAATTTTGTGTTATATATGGTAATCTTTATGATATTCGAATTATACACAGAACTTCCCTATTTGTAAAAAAAAGAGAAGAGGTGAGTTTCAAATACCACTCCTCCTTGATTAATTGATACTTTGAAAGGGGTTTCATATGGAATGAAAGGAATGAAAGAAAAGAACAAAGAAGGGATTTATGAAGGTTTAATTAATGAATCACTTCCCAATGGTATGTTTGGGGTTTGTTTAGATAATGAGGATCGAATTCTAGGTGACGTTTCAGGAAGGATTCGACATAGTTTTATACATATACTAGGAGTCAAAATTGCAGTAAGTTGTTACGATTCAACCAGAACCAGAGGGCGTATAGTTTAGAGACTACGAAACAAAGATTCGAATGATTAGGTGAAGTAGTCTTTTCAGTTGCAATATTCTTTTTTTAGGATCCAAGTCGAAATAGAAAATTTCAAGAAACTTATTTTCTTCCAATAAGTAGATTCGGAATTACGGTAAGGAATGACAAATATGAAAATAAGGGCCCTGATTCGTAAAATTTGTGAAAATGTCGACTAATCCGTAGGCGGAGACGAATTATGGTAATTTGTTCCAATCCGAGACATAAACAAAGACAAGGATAATCTTTATAAAAAAAGGACCCATAAGGATATACACATCCAAATAAATAAAGGATCCGTTTTGACATGAAATGGATATATCCATATATCTTTGACTTAGATTTATGAGATGATAAAATATGGCAAAACCCATACCAAGAATCGGTTCACGTAGAAATGGACGTATTAGCTCGCGTAAAAGTACGCGTAGAATACCAAAAGGCGTTATTCATGTTCAAGCAAGTTTAAACAATACAATTGTGACTGTTACAGATGTACGGGGTCGGGTAATTTCTTGGTCCTCCGCCGGTACTTGTGGATTCAAAGGTACAAGAAGAGGGACACCATTTGCCGCTCAAACCGCAGCAGGAAATGCTATTCGGGCAGTAGTGGATCAAGGTATGCAACGAGCAGAAGTGATGATAAAGGGCCCTGGTCTCGGAAGAGATGCAGCATTAAGAGCTATTCGTAGAAGCGGTATACTCTTAAGTTTTTCATACGGGATGTAACCCTATGCCACATAATGGCTGTAGGCCCCTAAAAACGACGTGTAAAAATAAACATTGAAGAGAAATTTCAAGAGAAATAAATAATTCAATGATTTTATCAAAAAATATTACTATGGTTCGAGAGAAAATAAGAGTATCGACTCGGACACTAAAGTGGAAGTGTGTTGAATCAAGAGCAGACAGTAAGCGTCTTTATTATGGACGCTTTATTCTGTCTCCACTTATGAAGGGTCAAGCCGATACTATAGGCATTGCGATGCGAAAAGCTTTGCTTGGAGAAATAGAGGGAACATGTATCACACGTGCAAAATCTGAAAAAATACCACATGAATACTCTACCATAGTCGGTATTCAAGAATCAGTACATGAAATTTTAATGAATTTGAAAGAAATTGTATTGAGAAGTAATCTGTATGGAACTCGTGATGCGTCTATTTGTGTAAAGGGTCCTGGATGCGTAACTGCTCAAGACATCATCTTACCACCTTCTGTGGAAATCATTGATAATACACAGCATATAGCTAACCTAACGGAGCCAATTAATTTGTGTATTGAATTAAAGATCGAGAGGAATCGCGGATATCGTATACAAACGCCAAATAATTTTCAAGACGCAAGTTATCCTATAGATGCTATATTCATGCCTGTTCGAAATGCGAATCATAGTATTCATTCTTATGGAAATGGGAATGAAAAACAAGAGATACTTTTTCTCGAAATATGGACCAATGGAAGTTTAACTCCTAAAGAAGCACTTCATGAAGCCTCTCGGAATTTGATTGATTTATTTATTCCGTTTTTACATCCGGAAGAAGAAAACTTCCATTTAAAAACCAATAAACAGAAAGTTACGTTACCTATTTTGACTTTTCATGAAAAATTGGCTAAACTAAGGAAAAAGAAAACCGAAATAGCATTAAAATATATTTTTATTGACCAATCTGAATTGCCTCCTAGGATCTATATTGCATTTCAAAAGGTCCAATATACATACATTATTGGACCTTTTGAATAATAGTCCAGACGAACTTATGAAAATGAAATATTTTCGCATAGAAGACGTAAAACATATATTAGACATTCTCGAAATGGAAAATAATTTTCTATAAATTTTGAATCCATTGGATTCTTTTTGTAGAAAAACTTTAGACTTTATAAAAGAAAACAAAAAAAGACTAAAACGAAGTTTGAATCTTTAACGGAATCTGTATACTCAAAATCAATCAAAAATTGAATTAAATATGTGTATCTAGGGGGACTTCACTTTGAAGCAACTATTCCCTAGATACACACGTCGTGATATTTTATTTATTTCGTAATTGAATCAATTTTAAAAAGACCTAAAGTTAGGGATTTATCAATCGGTAATGTTGCTCCAATACCTAACCAAAGGGCTACTGCGGTACCAATCAAAAAGACGGTTGTCGCTACTGGACGACGAAACGGATTTTGGAATTTATTGACATTCTCCAAAAAAGGTACAGTTAATAATCCCGCAGGTACGGAAACCATTAAAAGAACACCCAATAACTTATTTGGCACTGTACGAAGTATTTGAAATACGGGAAAGAAATACCATTCCGGCAATATTTCCAAAGGAGTTGCAAATGGATCTGCGGGTTCACCAATCATTGATGGTTCTAGAACCGCTAAGCCCACGTTACATGCAATAGTACCTAGAATTACTACTGGAAAAATATATAAAAGATCATTGGGCCATGCGGGTTCTCCGTAATAATTATGACCCATACCCTTAGCCAATTTAGCTCTTAATACAGGATCATTCAAATCTGGTTTTTTTGTTATTAGGATAGGTGAATTATTATGGATCCATCCCCCGAAGGAACCGGACATGATAATTTTTCATCATCCGGCTCGAGCACAAATCAAACGAACCAACTGGATCCAGATAAAAAATCGATATCTTATCCATATCCAAGCATATATAAAATAAATGATTGATTCAATGTAAGAAAAAATGTCACAGATTATGTTTTCCGGAGTTACAATTATCCATTGCAAGGAATTCAGTTCTTATAGATAAATGCTCAATACCCAAGTGGGCTTCCAAAGTTGATAATGTTCAAGTGCTTTATGGGTCGTCTCAGATCTTGCGATGGGGCTTTAG

mRNA sequence

ATGAAAGGAATGTCTTATCGAGGCAATCGTATTTGCTTCGGTAGATACGCTATTCAGGCACTTGAACCCGCTTGGATCACATCTAGACAAATCGAAGCGGGACGAAGGGCAATGACACGAAATGCACGACGCGGCGGAAAAATATGGGTACGTATATTTCCAGACAAACCCGTTACACTAAGACCCACAGAAACACGTATGGGTTCAGGGAAAGGATCCCCCGAATATTGGGTAGCTGTTATTAAACCAGATAACAGCGGAGCCCGAGAATTGATGTGTATTCGAATCATAGGGGCTAGTAATCGACGATATGCTCATATTGGTGACATTATTGTTGCTGTAATCAAGAAAGTTGTGATCAGAGCTGTAATTGTACGTACTTGTAAAGAACTCAAACGAGAAAACGGTATGATAATACGATATGATGACAATGCTGCGGTTGTTATTGATCAAGAAGGAAATCCAAAAGGAACTCGAATTTTTGGTGCGATTGCCCGAGAATTGAGACAAATCGGTTCACGTAGAAATGGACGTATTAGCTCGCGTAAAAGTACGCGTAGAATACCAAAAGGCGTTATTCATGTTCAAGCAAGTTTAAACAATACAATTGTGACTGTTACAGATGTACGGGGTCGGGTAATTTCTTGGTCCTCCGCCGGTACTTGTGGATTCAAAGGTACAAGAAGAGGGACACCATTTGCCGCTCAAACCGCAGCAGGAAATGCTATTCGGGCAGTAGTGGATCAAGGTATGCAACGAGCAGAAGTGATGATAAAGGGCCCTGGTCTCGGAAGAGATGCAGCATTAAGAGCTATTCGTAGAAGCGGTATACTCTTAAGTTTTTCATACGGGATTAAGCGTCTTTATTATGGACGCTTTATTCTGTCTCCACTTATGAAGGGTCAAGCCGATACTATAGGCATTGCGATGCGAAAAGCTTTGCTTGGAGAAATAGAGGGAACATGTATCACACGTGCAAAATCTGAAAAAATACCACATGAATACTCTACCATAGTCGGTATTCAAGAATCAGTACATGAAATTTTAATGAATTTGAAAGAAATTGTATTGAGAAGTAATCTGTATGGAACTCGTGATGCGTCTATTTGTGTAAAGGGTCCTGGATGCGTAACTGCTCAAGACATCATCTTACCACCTTCTGTGGAAATCATTGATAATACACAGCATATAGCTAACCTAACGGAGCCAATTAATTTGTGTATTGAATTAAAGATCGAGAGGAATCGCGGATATCGTATACAAACGCCAAATAATTTTCAAGACGCAAGTTATCCTATAGATGCTATATTCATGCCTGTTCGAAATGCGAATCATATTAATAATCCCGCAGATCTTGCGATGGGGCTTTAG

Coding sequence (CDS)

ATGAAAGGAATGTCTTATCGAGGCAATCGTATTTGCTTCGGTAGATACGCTATTCAGGCACTTGAACCCGCTTGGATCACATCTAGACAAATCGAAGCGGGACGAAGGGCAATGACACGAAATGCACGACGCGGCGGAAAAATATGGGTACGTATATTTCCAGACAAACCCGTTACACTAAGACCCACAGAAACACGTATGGGTTCAGGGAAAGGATCCCCCGAATATTGGGTAGCTGTTATTAAACCAGATAACAGCGGAGCCCGAGAATTGATGTGTATTCGAATCATAGGGGCTAGTAATCGACGATATGCTCATATTGGTGACATTATTGTTGCTGTAATCAAGAAAGTTGTGATCAGAGCTGTAATTGTACGTACTTGTAAAGAACTCAAACGAGAAAACGGTATGATAATACGATATGATGACAATGCTGCGGTTGTTATTGATCAAGAAGGAAATCCAAAAGGAACTCGAATTTTTGGTGCGATTGCCCGAGAATTGAGACAAATCGGTTCACGTAGAAATGGACGTATTAGCTCGCGTAAAAGTACGCGTAGAATACCAAAAGGCGTTATTCATGTTCAAGCAAGTTTAAACAATACAATTGTGACTGTTACAGATGTACGGGGTCGGGTAATTTCTTGGTCCTCCGCCGGTACTTGTGGATTCAAAGGTACAAGAAGAGGGACACCATTTGCCGCTCAAACCGCAGCAGGAAATGCTATTCGGGCAGTAGTGGATCAAGGTATGCAACGAGCAGAAGTGATGATAAAGGGCCCTGGTCTCGGAAGAGATGCAGCATTAAGAGCTATTCGTAGAAGCGGTATACTCTTAAGTTTTTCATACGGGATTAAGCGTCTTTATTATGGACGCTTTATTCTGTCTCCACTTATGAAGGGTCAAGCCGATACTATAGGCATTGCGATGCGAAAAGCTTTGCTTGGAGAAATAGAGGGAACATGTATCACACGTGCAAAATCTGAAAAAATACCACATGAATACTCTACCATAGTCGGTATTCAAGAATCAGTACATGAAATTTTAATGAATTTGAAAGAAATTGTATTGAGAAGTAATCTGTATGGAACTCGTGATGCGTCTATTTGTGTAAAGGGTCCTGGATGCGTAACTGCTCAAGACATCATCTTACCACCTTCTGTGGAAATCATTGATAATACACAGCATATAGCTAACCTAACGGAGCCAATTAATTTGTGTATTGAATTAAAGATCGAGAGGAATCGCGGATATCGTATACAAACGCCAAATAATTTTCAAGACGCAAGTTATCCTATAGATGCTATATTCATGCCTGTTCGAAATGCGAATCATATTAATAATCCCGCAGATCTTGCGATGGGGCTTTAG

Protein sequence

MKGMSYRGNRICFGRYAIQALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVTLRPTETRMGSGKGSPEYWVAVIKPDNSGARELMCIRIIGASNRRYAHIGDIIVAVIKKVVIRAVIVRTCKELKRENGMIIRYDDNAAVVIDQEGNPKGTRIFGAIARELRQIGSRRNGRISSRKSTRRIPKGVIHVQASLNNTIVTVTDVRGRVISWSSAGTCGFKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGILLSFSYGIKRLYYGRFILSPLMKGQADTIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIIDNTQHIANLTEPINLCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANHINNPADLAMGL
Homology
BLAST of Sed0026352 vs. NCBI nr
Match: RDY14681.1 (DNA-directed RNA polymerase subunit alpha, partial [Mucuna pruriens])

HSP 1 Score: 688.7 bits (1776), Expect = 3.3e-194
Identity = 364/490 (74.29%), Postives = 391/490 (79.80%), Query Frame = 0

Query: 1   MKGMSYRGNRICFGRYAIQALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVTL 60
           MKG+SYRGN ICFGRYA+QALEPAWITSRQIEAGRRAM+RN RRGG+IWVRIFPDKPVT+
Sbjct: 1   MKGISYRGNLICFGRYALQALEPAWITSRQIEAGRRAMSRNVRRGGQIWVRIFPDKPVTV 60

Query: 61  RPTETRMGSGKGSPEYWVAVIKP-----------------------------------DN 120
           RPTETRMGSGKGSPEYWVAV+KP                                     
Sbjct: 61  RPTETRMGSGKGSPEYWVAVVKPGKILYEMGGKGYFNSSIQNAYTNSIHYFWIIIRTKGK 120

Query: 121 SGARELMCIRIIGASNRRYAHIGDIIVAVIKKV----------VIRAVIVRTCKELKREN 180
           +GARELMCIRI+GASNRRYA+IGDI+VAVIK+           VIRAVIVRTCKELKR N
Sbjct: 121 NGARELMCIRILGASNRRYAYIGDIVVAVIKEAVPNTNLERSEVIRAVIVRTCKELKRSN 180

Query: 181 GMIIRYDDNAAVVIDQEGNPKGTRIFGAIARELRQIGSRRNGRISSRKSTRRIPKGVIHV 240
           G+II+YDDNAAVVIDQEGNPKGTRIF AIARELRQ+   +   ++  + TR+IPKGVIHV
Sbjct: 181 GIIIQYDDNAAVVIDQEGNPKGTRIFCAIARELRQLNFTKIVSLAP-EHTRKIPKGVIHV 240

Query: 241 QASLNNTIVTVTDVRGRVISWSSAGTCGFKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAE 300
           QAS NNTIVTVTDVRGRVISWSSAGTCGFKGTRRGTPF AQTAAGNAIR V DQGMQRAE
Sbjct: 241 QASFNNTIVTVTDVRGRVISWSSAGTCGFKGTRRGTPFGAQTAAGNAIRTVADQGMQRAE 300

Query: 301 VMIKGPGLGRDAALRAIRRSGILLSFSYGIKRLYYGRFILSPLMKGQADTIGIAMRKALL 360
           VMIKGPGLGRDAALRAIRRS                         GQADTIGIAMR+ LL
Sbjct: 301 VMIKGPGLGRDAALRAIRRS-------------------------GQADTIGIAMRRVLL 360

Query: 361 GEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDASICVKGPG 420
           GEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGT+DASIC KGPG
Sbjct: 361 GEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTQDASICFKGPG 420

Query: 421 CVTAQDIILPPSVEIIDNTQHIANLTEPINLCIELKIERNRGYRIQTPNNFQDASYPIDA 446
            VTAQDIILPPSVEI+DNTQHIAN+TE +NLCI L+IERNRGYRI+T  NFQD SYPIDA
Sbjct: 421 YVTAQDIILPPSVEIVDNTQHIANVTESVNLCIGLQIERNRGYRIKTLKNFQDGSYPIDA 464

BLAST of Sed0026352 vs. NCBI nr
Match: KAD4981935.1 (hypothetical protein E3N88_18606 [Mikania micrantha])

HSP 1 Score: 609.8 bits (1571), Expect = 2.0e-170
Identity = 334/476 (70.17%), Postives = 359/476 (75.42%), Query Frame = 0

Query: 20  ALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVTLRPTETRMGSGKGSPEYWVA 79
           ALEPAWITSRQIEAGRRAMT NARRGGKIWVRIFPDKPVT+RP ETRMGSGKG PEYWVA
Sbjct: 255 ALEPAWITSRQIEAGRRAMTLNARRGGKIWVRIFPDKPVTVRPAETRMGSGKGFPEYWVA 314

Query: 80  VIKP-------DNSGARELMCIRIIGASNRRYAHIGDIIVAVIKKV----------VIRA 139
           V+KP       DNSGARELMCIRIIGASNRRYAHIGD+IVAVIK            V+RA
Sbjct: 315 VVKPGRILYEMDNSGARELMCIRIIGASNRRYAHIGDVIVAVIKDAVPNMPLERSEVVRA 374

Query: 140 VIVRTCKELKRENGMIIRYDDNAAVVIDQEGNPKGTRIFGAIARELRQI----------- 199
           VIVRTCKELKR+NGMIIRYDDNAAVVIDQEGNPKGTR+FGAIARELRQ            
Sbjct: 375 VIVRTCKELKRDNGMIIRYDDNAAVVIDQEGNPKGTRVFGAIARELRQFNFTKIVSLAPE 434

Query: 200 ----GSRRNGRISSRKSTRRIPKGVIHVQASLNNTIV----------------------- 259
               G +    I     T  +P G+  V+    N I+                       
Sbjct: 435 EVLPGMKEQKWIHEGLITESLPNGMFRVRLDNENMILGYVSGKIRRSFIRILPGDRVKIE 494

Query: 260 -----TVTDVRGRVISWSSAGTCGFKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIK 319
                +  DVRGRV+SWSSAGTCGF+GTRRGTPFA QTAA NAIRAVVDQGMQRAEVMIK
Sbjct: 495 VSRYDSTRDVRGRVVSWSSAGTCGFRGTRRGTPFATQTAAANAIRAVVDQGMQRAEVMIK 554

Query: 320 GPGLGRDAALRAIRRSGILLSFSY-------GIKRLYYGRFILSPLMKGQADTIGIAMRK 379
           GPGL RDAALRAIRRS    +  +         KRL YGRFILSPLMKGQADTIGIAMR+
Sbjct: 555 GPGLRRDAALRAIRRSVSTRTLQWKCVESAADSKRLLYGRFILSPLMKGQADTIGIAMRR 614

Query: 380 ALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDASICVK 429
           ALLGEIEGTCITRAKSEKI HEYSTI+GIQESVHEILMNLKEIVLRSNLYGT +ASICV+
Sbjct: 615 ALLGEIEGTCITRAKSEKISHEYSTIMGIQESVHEILMNLKEIVLRSNLYGTCEASICVR 674

BLAST of Sed0026352 vs. NCBI nr
Match: KAF8376862.1 (hypothetical protein HHK36_031458 [Tetracentron sinense] >KAF8398788.1 hypothetical protein HHK36_014646 [Tetracentron sinense])

HSP 1 Score: 588.6 bits (1516), Expect = 4.7e-164
Identity = 316/404 (78.22%), Postives = 335/404 (82.92%), Query Frame = 0

Query: 92  MCIRIIGASNRRYAHIGDIIVAVIKKV----------VIRAVIVRTCKELKRENGMIIRY 151
           MCIRIIGASNRRYAHIGD+IVAVIK+           VIRAVIVRTCKELKR+NGMIIRY
Sbjct: 1   MCIRIIGASNRRYAHIGDVIVAVIKEAVPNMPLERSEVIRAVIVRTCKELKRDNGMIIRY 60

Query: 152 DDNAAVVIDQEGNPKGTRIFGAIARELRQ------------------------------- 211
           DDNAAVVIDQEGNPKGTR+FGAIARELRQ                               
Sbjct: 61  DDNAAVVIDQEGNPKGTRVFGAIARELRQLNFTKIVSLAPEEGSDVVLYGYYQEIESKSK 120

Query: 212 IGSRRNGRISSRKSTRRIPKGVIHVQASLNNTIVTVTDVRGRVISWSSAGTCGFKGTRRG 271
           IGSR NGRI SRK++RRIPKGVIHVQAS NNTIVTVTDVRGRVISWSSAGTCGFKGTRRG
Sbjct: 121 IGSRGNGRIGSRKNSRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCGFKGTRRG 180

Query: 272 TPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGILLSF--------- 331
           TPFAAQTAAGN IR VVDQGMQRAEVMIKGPGLGRDAALRAIRRSGILLSF         
Sbjct: 181 TPFAAQTAAGNVIRTVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGILLSFVRDWKCVES 240

Query: 332 SYGIKRLYYGRFILSPLMKGQADTIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGI 391
               KRLYYGRFILSPLMKGQADTIGIAMR+ALLGEIEGTCITRAKSEKIPHEYSTIVGI
Sbjct: 241 RADSKRLYYGRFILSPLMKGQADTIGIAMRRALLGEIEGTCITRAKSEKIPHEYSTIVGI 300

Query: 392 QESVHEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIIDNTQHIANLT 446
           +ESV EIL+NLKEIVLRSNLYGT DASICV+GPG  TAQDII PPSVEI+D TQHIA+LT
Sbjct: 301 EESVQEILLNLKEIVLRSNLYGTLDASICVRGPGYATAQDIISPPSVEIVDATQHIASLT 360

BLAST of Sed0026352 vs. NCBI nr
Match: KAD7117051.1 (hypothetical protein E3N88_04319 [Mikania micrantha])

HSP 1 Score: 587.8 bits (1514), Expect = 8.0e-164
Identity = 323/461 (70.07%), Postives = 346/461 (75.05%), Query Frame = 0

Query: 20  ALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVTLRPTETRMGSGKGSPEYWVA 79
           ALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVT+RPTETRMGSGKGSPEYWVA
Sbjct: 202 ALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVTVRPTETRMGSGKGSPEYWVA 261

Query: 80  VIKP-------DNSGARELMCIRIIGASNRRYAHIGDIIVAVIKKV----------VIRA 139
           V+KP       DNSGARELMCIRIIGASNRRYAHIGD+IVAVIK            V+RA
Sbjct: 262 VVKPGRILYEMDNSGARELMCIRIIGASNRRYAHIGDVIVAVIKDAVPNMPLERSEVVRA 321

Query: 140 VIVRTCKELKRENGMIIRYDDNAAVVIDQEGNPKGTRIFGAIARELRQI----------- 199
           VIVRTCKELKR+NGMIIRYDDN AVVIDQEGNPKGTR FGAIARELRQ            
Sbjct: 322 VIVRTCKELKRDNGMIIRYDDNVAVVIDQEGNPKGTRFFGAIARELRQFNFTKIVSLAPE 381

Query: 200 GSRRNGRISSRKSTRRIPKGVIHVQASLNNTIVTVTDVRGRVISWSSAGTCGFKGTRRGT 259
           GSR  GRI SRKS R+IPKGVIH+QAS NNTIVTVTDVRG+V+SWSSAGTCGF+GTRRGT
Sbjct: 382 GSR--GRIGSRKSIRKIPKGVIHIQASFNNTIVTVTDVRGQVVSWSSAGTCGFRGTRRGT 441

Query: 260 PFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGILLSFSY-------G 319
           PFAAQTAA NAIRAVVDQGMQRAEVMIKGPGLGRDA LRAIRRS    +  +        
Sbjct: 442 PFAAQTAAANAIRAVVDQGMQRAEVMIKGPGLGRDATLRAIRRSVSTRTLQWKCVESAAD 501

Query: 320 IKRLYYGRFILSPLMKGQADTIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQES 379
            KRL YGRFILS LMKGQADTIGIAMR+A        C+ ++K                 
Sbjct: 502 SKRLLYGRFILSSLMKGQADTIGIAMRRA--------CLEKSKEH--------------- 561

Query: 380 VHEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIIDNTQHIANLTEPI 439
                                 ASICV+GPG VTAQDIILPP VEI+DNTQHIA+LTEPI
Sbjct: 562 ----------------------ASICVRGPGYVTAQDIILPPYVEIVDNTQHIASLTEPI 615

Query: 440 NLCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANH 446
            L I L+IE+NRGY I+ PN FQD SYPID +FMPVRNANH
Sbjct: 622 ELVIGLQIEKNRGYLIKAPNTFQDGSYPIDPVFMPVRNANH 615

BLAST of Sed0026352 vs. NCBI nr
Match: KAF9660677.1 (hypothetical protein SADUNF_SadunfPtG0000200 [Salix dunnii] >KAF9664285.1 hypothetical protein SADUNF_Sadunf16G0002000 [Salix dunnii])

HSP 1 Score: 547.0 bits (1408), Expect = 1.6e-151
Identity = 302/471 (64.12%), Postives = 322/471 (68.37%), Query Frame = 0

Query: 1   MKGMSYRGNRICFGRYAIQALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVTL 60
           MKG+SYRGNRICFGRYA+QALEPAWITSRQIEAGRRAMTRN RRGGKIWVRIFPDKPVTL
Sbjct: 1   MKGVSYRGNRICFGRYALQALEPAWITSRQIEAGRRAMTRNVRRGGKIWVRIFPDKPVTL 60

Query: 61  RPTETRMGSGKGSPEYWVAVIKP----------------DNSGARELMCIRIIGASNRRY 120
           RP+ETRMGSGKGSPEYWVAV+KP                DNSGARELMCIRIIG SNRRY
Sbjct: 61  RPSETRMGSGKGSPEYWVAVVKPDSKKNMIQSQTHLNVADNSGARELMCIRIIGTSNRRY 120

Query: 121 AHIGDIIVAVIKKV----------VIRAVIVRTCKELKRENGMIIRYDDNAAVVIDQEGN 180
           AHIGD+I+AVIK+           VIRAVIVRT KELKR+N                   
Sbjct: 121 AHIGDVIIAVIKEAVPNSPLERSEVIRAVIVRTSKELKRDN------------------- 180

Query: 181 PKGTRIFGAIARELRQIGSRRNGRISSRKSTRRIPKGVIHVQASLNNTIVTVTDVRGRVI 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 SWSSAGTCGFKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRR 300
                G CGF+GTRRGTPFAAQTAAGNAIR VVDQGMQRAEVMIKGPGLGRDAALRAIRR
Sbjct: 241 -----GACGFRGTRRGTPFAAQTAAGNAIRTVVDQGMQRAEVMIKGPGLGRDAALRAIRR 300

Query: 301 SGILLSFSYGIKRLYYGRFILSPLMKGQADTIGIAMRKALLGEIEGTCITRAKSEKIPHE 360
           S                         GQADTIGIAMR+ALLGEIEGTCITRAKS+KIPHE
Sbjct: 301 S-------------------------GQADTIGIAMRRALLGEIEGTCITRAKSQKIPHE 360

Query: 361 YSTIVGIQESVHEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIIDNT 420
           +STI GIQES+HEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIIDNT
Sbjct: 361 FSTITGIQESIHEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIIDNT 362

Query: 421 QHIANLTEPINLCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANH 446
           QHIA+L EPI+L I L+IERNRGY +++P NFQD SY IDA+FMPVRNANH
Sbjct: 421 QHIASLREPIDLHIGLQIERNRGYCMKSPKNFQDGSYSIDAVFMPVRNANH 362

BLAST of Sed0026352 vs. ExPASy Swiss-Prot
Match: Q4VZK3 (DNA-directed RNA polymerase subunit alpha OS=Cucumis sativus OX=3659 GN=rpoA PE=3 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 7.7e-85
Identity = 154/160 (96.25%), Postives = 156/160 (97.50%), Query Frame = 0

Query: 286 KRLYYGRFILSPLMKGQADTIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESV 345
           KRLYYGRFILSPLMKGQ DTIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESV
Sbjct: 25  KRLYYGRFILSPLMKGQGDTIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESV 84

Query: 346 HEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIIDNTQHIANLTEPIN 405
           HEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEI+DNTQHIANL EPIN
Sbjct: 85  HEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIVDNTQHIANLMEPIN 144

Query: 406 LCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANH 446
           LCIELKIERNRGY IQTPNNFQDASYP+DAIFMPVRN NH
Sbjct: 145 LCIELKIERNRGYHIQTPNNFQDASYPMDAIFMPVRNVNH 184

BLAST of Sed0026352 vs. ExPASy Swiss-Prot
Match: Q09WY5 (DNA-directed RNA polymerase subunit alpha OS=Morus indica OX=248361 GN=rpoA PE=3 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 4.7e-82
Identity = 148/160 (92.50%), Postives = 157/160 (98.12%), Query Frame = 0

Query: 286 KRLYYGRFILSPLMKGQADTIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESV 345
           KRLYYGRF+L+PLM+GQADTIGIAMR+ALLGEIEGTCITRAKSEKIPHEYSTIVGIQESV
Sbjct: 25  KRLYYGRFLLAPLMRGQADTIGIAMRRALLGEIEGTCITRAKSEKIPHEYSTIVGIQESV 84

Query: 346 HEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIIDNTQHIANLTEPIN 405
           HEILMNLKEIVLRSNLYGTRDASICVKGPG VTAQDIILPPSVEI+DNTQHIANLTEPI 
Sbjct: 85  HEILMNLKEIVLRSNLYGTRDASICVKGPGYVTAQDIILPPSVEIVDNTQHIANLTEPIT 144

Query: 406 LCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANH 446
           LCIEL+IERNRGYRI+TPNNFQ+ SYPIDA+FMPVRNANH
Sbjct: 145 LCIELQIERNRGYRIKTPNNFQNGSYPIDAVFMPVRNANH 184

BLAST of Sed0026352 vs. ExPASy Swiss-Prot
Match: Q49KW6 (DNA-directed RNA polymerase subunit alpha OS=Eucalyptus globulus subsp. globulus OX=71271 GN=rpoA PE=3 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.5e-80
Identity = 147/160 (91.88%), Postives = 154/160 (96.25%), Query Frame = 0

Query: 286 KRLYYGRFILSPLMKGQADTIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESV 345
           KRLYYGRFILSPLMKGQADTIGIAMR+ALLGEIEGTCITRAK EKIPHEYSTIVGIQESV
Sbjct: 25  KRLYYGRFILSPLMKGQADTIGIAMRRALLGEIEGTCITRAKFEKIPHEYSTIVGIQESV 84

Query: 346 HEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIIDNTQHIANLTEPIN 405
           HEILMNLKEIVLRSNLYGTR+ASICVKGPGCVTAQDIILP SVEI+DNTQ+IANLTEPIN
Sbjct: 85  HEILMNLKEIVLRSNLYGTRNASICVKGPGCVTAQDIILPTSVEIVDNTQYIANLTEPIN 144

Query: 406 LCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANH 446
           LCI L+IERNRGY I+TP NFQD SYPIDA+FMPVRNANH
Sbjct: 145 LCIGLQIERNRGYHIKTPKNFQDGSYPIDAVFMPVRNANH 184

BLAST of Sed0026352 vs. ExPASy Swiss-Prot
Match: Q9MTJ3 (DNA-directed RNA polymerase subunit alpha OS=Oenothera elata subsp. hookeri OX=85636 GN=rpoA PE=3 SV=2)

HSP 1 Score: 298.1 bits (762), Expect = 1.7e-79
Identity = 145/160 (90.62%), Postives = 154/160 (96.25%), Query Frame = 0

Query: 286 KRLYYGRFILSPLMKGQADTIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESV 345
           KRLYYGRFILSPLMKGQADTIGIAMR+ALLGEIEGTCITRAK EKIPHEYSTIVGIQESV
Sbjct: 25  KRLYYGRFILSPLMKGQADTIGIAMRRALLGEIEGTCITRAKFEKIPHEYSTIVGIQESV 84

Query: 346 HEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIIDNTQHIANLTEPIN 405
           HEILMNLKEIVLRSNLYGT +ASICVKGPGCVTAQDI+LPPSVEIIDNTQHIA+L EPI+
Sbjct: 85  HEILMNLKEIVLRSNLYGTCNASICVKGPGCVTAQDILLPPSVEIIDNTQHIASLMEPIH 144

Query: 406 LCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANH 446
           LCI L+IERNRGY I++PNNFQD SYPIDA+FMPVRNANH
Sbjct: 145 LCIGLQIERNRGYHIKSPNNFQDGSYPIDAVFMPVRNANH 184

BLAST of Sed0026352 vs. ExPASy Swiss-Prot
Match: Q2PMQ3 (DNA-directed RNA polymerase subunit alpha OS=Glycine max OX=3847 GN=rpoA PE=3 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.7e-79
Identity = 144/160 (90.00%), Postives = 151/160 (94.38%), Query Frame = 0

Query: 286 KRLYYGRFILSPLMKGQADTIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESV 345
           KRLYYGRFILSPLMKGQADTIGIAMR+ LLGEIEGTCITR KSEKIPHEYSTI+GI+ESV
Sbjct: 25  KRLYYGRFILSPLMKGQADTIGIAMRRVLLGEIEGTCITRVKSEKIPHEYSTIIGIEESV 84

Query: 346 HEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIIDNTQHIANLTEPIN 405
           HEILMNLKEIVLRSNLYGTRDASIC KGPG VTAQDIILPPSVEI+DNTQHIAN+TEP+N
Sbjct: 85  HEILMNLKEIVLRSNLYGTRDASICFKGPGYVTAQDIILPPSVEIVDNTQHIANVTEPVN 144

Query: 406 LCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANH 446
           LCIELKIERNRGYRI+T  NFQD SYPIDA FMPVRN NH
Sbjct: 145 LCIELKIERNRGYRIKTLKNFQDGSYPIDATFMPVRNVNH 184

BLAST of Sed0026352 vs. ExPASy TrEMBL
Match: A0A371II16 (Plastid-encoded RNA polymerase subunit alpha (Fragment) OS=Mucuna pruriens OX=157652 GN=rpoA PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.6e-194
Identity = 364/490 (74.29%), Postives = 391/490 (79.80%), Query Frame = 0

Query: 1   MKGMSYRGNRICFGRYAIQALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVTL 60
           MKG+SYRGN ICFGRYA+QALEPAWITSRQIEAGRRAM+RN RRGG+IWVRIFPDKPVT+
Sbjct: 1   MKGISYRGNLICFGRYALQALEPAWITSRQIEAGRRAMSRNVRRGGQIWVRIFPDKPVTV 60

Query: 61  RPTETRMGSGKGSPEYWVAVIKP-----------------------------------DN 120
           RPTETRMGSGKGSPEYWVAV+KP                                     
Sbjct: 61  RPTETRMGSGKGSPEYWVAVVKPGKILYEMGGKGYFNSSIQNAYTNSIHYFWIIIRTKGK 120

Query: 121 SGARELMCIRIIGASNRRYAHIGDIIVAVIKKV----------VIRAVIVRTCKELKREN 180
           +GARELMCIRI+GASNRRYA+IGDI+VAVIK+           VIRAVIVRTCKELKR N
Sbjct: 121 NGARELMCIRILGASNRRYAYIGDIVVAVIKEAVPNTNLERSEVIRAVIVRTCKELKRSN 180

Query: 181 GMIIRYDDNAAVVIDQEGNPKGTRIFGAIARELRQIGSRRNGRISSRKSTRRIPKGVIHV 240
           G+II+YDDNAAVVIDQEGNPKGTRIF AIARELRQ+   +   ++  + TR+IPKGVIHV
Sbjct: 181 GIIIQYDDNAAVVIDQEGNPKGTRIFCAIARELRQLNFTKIVSLAP-EHTRKIPKGVIHV 240

Query: 241 QASLNNTIVTVTDVRGRVISWSSAGTCGFKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAE 300
           QAS NNTIVTVTDVRGRVISWSSAGTCGFKGTRRGTPF AQTAAGNAIR V DQGMQRAE
Sbjct: 241 QASFNNTIVTVTDVRGRVISWSSAGTCGFKGTRRGTPFGAQTAAGNAIRTVADQGMQRAE 300

Query: 301 VMIKGPGLGRDAALRAIRRSGILLSFSYGIKRLYYGRFILSPLMKGQADTIGIAMRKALL 360
           VMIKGPGLGRDAALRAIRRS                         GQADTIGIAMR+ LL
Sbjct: 301 VMIKGPGLGRDAALRAIRRS-------------------------GQADTIGIAMRRVLL 360

Query: 361 GEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDASICVKGPG 420
           GEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGT+DASIC KGPG
Sbjct: 361 GEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTQDASICFKGPG 420

Query: 421 CVTAQDIILPPSVEIIDNTQHIANLTEPINLCIELKIERNRGYRIQTPNNFQDASYPIDA 446
            VTAQDIILPPSVEI+DNTQHIAN+TE +NLCI L+IERNRGYRI+T  NFQD SYPIDA
Sbjct: 421 YVTAQDIILPPSVEIVDNTQHIANVTESVNLCIGLQIERNRGYRIKTLKNFQDGSYPIDA 464

BLAST of Sed0026352 vs. ExPASy TrEMBL
Match: A0A5N6NML1 (S1-like domain-containing protein OS=Mikania micrantha OX=192012 GN=E3N88_18606 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 9.6e-171
Identity = 334/476 (70.17%), Postives = 359/476 (75.42%), Query Frame = 0

Query: 20  ALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVTLRPTETRMGSGKGSPEYWVA 79
           ALEPAWITSRQIEAGRRAMT NARRGGKIWVRIFPDKPVT+RP ETRMGSGKG PEYWVA
Sbjct: 255 ALEPAWITSRQIEAGRRAMTLNARRGGKIWVRIFPDKPVTVRPAETRMGSGKGFPEYWVA 314

Query: 80  VIKP-------DNSGARELMCIRIIGASNRRYAHIGDIIVAVIKKV----------VIRA 139
           V+KP       DNSGARELMCIRIIGASNRRYAHIGD+IVAVIK            V+RA
Sbjct: 315 VVKPGRILYEMDNSGARELMCIRIIGASNRRYAHIGDVIVAVIKDAVPNMPLERSEVVRA 374

Query: 140 VIVRTCKELKRENGMIIRYDDNAAVVIDQEGNPKGTRIFGAIARELRQI----------- 199
           VIVRTCKELKR+NGMIIRYDDNAAVVIDQEGNPKGTR+FGAIARELRQ            
Sbjct: 375 VIVRTCKELKRDNGMIIRYDDNAAVVIDQEGNPKGTRVFGAIARELRQFNFTKIVSLAPE 434

Query: 200 ----GSRRNGRISSRKSTRRIPKGVIHVQASLNNTIV----------------------- 259
               G +    I     T  +P G+  V+    N I+                       
Sbjct: 435 EVLPGMKEQKWIHEGLITESLPNGMFRVRLDNENMILGYVSGKIRRSFIRILPGDRVKIE 494

Query: 260 -----TVTDVRGRVISWSSAGTCGFKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIK 319
                +  DVRGRV+SWSSAGTCGF+GTRRGTPFA QTAA NAIRAVVDQGMQRAEVMIK
Sbjct: 495 VSRYDSTRDVRGRVVSWSSAGTCGFRGTRRGTPFATQTAAANAIRAVVDQGMQRAEVMIK 554

Query: 320 GPGLGRDAALRAIRRSGILLSFSY-------GIKRLYYGRFILSPLMKGQADTIGIAMRK 379
           GPGL RDAALRAIRRS    +  +         KRL YGRFILSPLMKGQADTIGIAMR+
Sbjct: 555 GPGLRRDAALRAIRRSVSTRTLQWKCVESAADSKRLLYGRFILSPLMKGQADTIGIAMRR 614

Query: 380 ALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDASICVK 429
           ALLGEIEGTCITRAKSEKI HEYSTI+GIQESVHEILMNLKEIVLRSNLYGT +ASICV+
Sbjct: 615 ALLGEIEGTCITRAKSEKISHEYSTIMGIQESVHEILMNLKEIVLRSNLYGTCEASICVR 674

BLAST of Sed0026352 vs. ExPASy TrEMBL
Match: A0A5N6PW93 (Ribosomal_L2_C domain-containing protein OS=Mikania micrantha OX=192012 GN=E3N88_04319 PE=3 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 3.9e-164
Identity = 323/461 (70.07%), Postives = 346/461 (75.05%), Query Frame = 0

Query: 20  ALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVTLRPTETRMGSGKGSPEYWVA 79
           ALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVT+RPTETRMGSGKGSPEYWVA
Sbjct: 202 ALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVTVRPTETRMGSGKGSPEYWVA 261

Query: 80  VIKP-------DNSGARELMCIRIIGASNRRYAHIGDIIVAVIKKV----------VIRA 139
           V+KP       DNSGARELMCIRIIGASNRRYAHIGD+IVAVIK            V+RA
Sbjct: 262 VVKPGRILYEMDNSGARELMCIRIIGASNRRYAHIGDVIVAVIKDAVPNMPLERSEVVRA 321

Query: 140 VIVRTCKELKRENGMIIRYDDNAAVVIDQEGNPKGTRIFGAIARELRQI----------- 199
           VIVRTCKELKR+NGMIIRYDDN AVVIDQEGNPKGTR FGAIARELRQ            
Sbjct: 322 VIVRTCKELKRDNGMIIRYDDNVAVVIDQEGNPKGTRFFGAIARELRQFNFTKIVSLAPE 381

Query: 200 GSRRNGRISSRKSTRRIPKGVIHVQASLNNTIVTVTDVRGRVISWSSAGTCGFKGTRRGT 259
           GSR  GRI SRKS R+IPKGVIH+QAS NNTIVTVTDVRG+V+SWSSAGTCGF+GTRRGT
Sbjct: 382 GSR--GRIGSRKSIRKIPKGVIHIQASFNNTIVTVTDVRGQVVSWSSAGTCGFRGTRRGT 441

Query: 260 PFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGILLSFSY-------G 319
           PFAAQTAA NAIRAVVDQGMQRAEVMIKGPGLGRDA LRAIRRS    +  +        
Sbjct: 442 PFAAQTAAANAIRAVVDQGMQRAEVMIKGPGLGRDATLRAIRRSVSTRTLQWKCVESAAD 501

Query: 320 IKRLYYGRFILSPLMKGQADTIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQES 379
            KRL YGRFILS LMKGQADTIGIAMR+A        C+ ++K                 
Sbjct: 502 SKRLLYGRFILSSLMKGQADTIGIAMRRA--------CLEKSKEH--------------- 561

Query: 380 VHEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIIDNTQHIANLTEPI 439
                                 ASICV+GPG VTAQDIILPP VEI+DNTQHIA+LTEPI
Sbjct: 562 ----------------------ASICVRGPGYVTAQDIILPPYVEIVDNTQHIASLTEPI 615

Query: 440 NLCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANH 446
            L I L+IE+NRGY I+ PN FQD SYPID +FMPVRNANH
Sbjct: 622 ELVIGLQIEKNRGYLIKAPNTFQDGSYPIDPVFMPVRNANH 615

BLAST of Sed0026352 vs. ExPASy TrEMBL
Match: A0A5N6N9A8 (S1-like domain-containing protein OS=Mikania micrantha OX=192012 GN=E3N88_22057 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 2.6e-144
Identity = 288/422 (68.25%), Postives = 312/422 (73.93%), Query Frame = 0

Query: 84  DNSGARELMCIRIIGASNRRYAHIGDIIVAVIKKV----------VIRAVIVRTCKELKR 143
           DNSGARELMCIRIIGASNRRYAHIGD+IVAVIK            V+RAVIVRTCKELKR
Sbjct: 12  DNSGARELMCIRIIGASNRRYAHIGDVIVAVIKDAVPNMPLERSEVVRAVIVRTCKELKR 71

Query: 144 ENGMIIRYDDNAAVVIDQEGNPKGTRIFGAIARELRQI---------------GSRRNGR 203
           +NGMIIRYDDNAAVVIDQEGNPKGTR+FGAIARELRQ                G +    
Sbjct: 72  DNGMIIRYDDNAAVVIDQEGNPKGTRVFGAIARELRQFNFTKIVSLAPEEVLPGMKEQKW 131

Query: 204 ISSRKSTRRIPKGVIHVQASLNNTIV----------------------------TVTDVR 263
           I     T  +P G+  V+    N I+                            +  DVR
Sbjct: 132 IHEGLITESLPNGMFRVRLDNENMILGYVSGKIRRSFIRILPGDRVKIEVSRYDSTRDVR 191

Query: 264 GRVISWSSAGTCGFKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALR 323
           GRV+SWSSAGTCGF+GTRRGTPFAAQTAA NAIRAVVDQGMQRAEVMIKGPGLGRDA LR
Sbjct: 192 GRVVSWSSAGTCGFRGTRRGTPFAAQTAAANAIRAVVDQGMQRAEVMIKGPGLGRDATLR 251

Query: 324 AIRRSGILLSFSY-------GIKRLYYGRFILSPLMKGQADTIGIAMRKALLGEIEGTCI 383
           AIRRS    +  +         KRL YGRFILSPLMKGQADTIGIAMR+ALLGEIEGTCI
Sbjct: 252 AIRRSVSTRTLQWKCVESAADSKRLLYGRFILSPLMKGQADTIGIAMRRALLGEIEGTCI 311

Query: 384 TRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDII 443
           TRAK EKI HEYSTI+GIQESVHEILMNLKEIVLRSNLYGT +ASICV+GPG VTAQDII
Sbjct: 312 TRAKFEKISHEYSTIMGIQESVHEILMNLKEIVLRSNLYGTCEASICVRGPGYVTAQDII 371

Query: 444 LPPSVEIIDNTQHIANLTEPINLCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNA 446
           LPP V I+DNTQHIA+LTEPI L I L+IE+NRGY I+ PN FQD SYPID +FMPVRNA
Sbjct: 372 LPPYVAIVDNTQHIASLTEPIELVIGLQIEKNRGYLIKAPNTFQDGSYPIDPVFMPVRNA 431

BLAST of Sed0026352 vs. ExPASy TrEMBL
Match: A0A6A5PE04 (RPOLD domain-containing protein OS=Lupinus albus OX=3870 GN=Lal_00046743 PE=3 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 4.5e-136
Identity = 273/391 (69.82%), Postives = 297/391 (75.96%), Query Frame = 0

Query: 84  DNSGARELMCIRIIGASNRRYAHIGDIIVAVIKKV----------VIRAVIVRTCKELKR 143
           +NSGARELMCIRIIGASN+RY +IGDI+VAVIK+           VIR VIVRTCKELKR
Sbjct: 12  NNSGARELMCIRIIGASNQRYGYIGDIVVAVIKEAVPNTPLERSEVIRTVIVRTCKELKR 71

Query: 144 ENGMIIRYDDNAAVVIDQEGNPKGTRIFGAIARELRQI-------------------GSR 203
            NG+II Y+DNAAV+IDQEGNPKGTRIFGA+ARELRQ+                   GS 
Sbjct: 72  NNGIIIHYNDNAAVIIDQEGNPKGTRIFGAMARELRQLNFTKIVSLASEYGKPTPKMGSC 131

Query: 204 RNGRISSRKSTRRIPKGVIHVQASLNNTIVTVTDVRGRVISWSSAGTCGFKGTRRGTPFA 263
           +NGRI SRK  R+IPKGVIHVQAS NNTIVTVTDVRG VIS SS GTCGFKGTRRG  FA
Sbjct: 132 KNGRIGSRKHARKIPKGVIHVQASFNNTIVTVTDVRGWVISCSSTGTCGFKGTRRGKSFA 191

Query: 264 AQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGILLSFSYGIKRLYYGRFI 323
           AQT   NAI  V DQGM+RAEVMIKGPGLG DAALRAIRRS                   
Sbjct: 192 AQTTIANAILRVADQGMERAEVMIKGPGLG-DAALRAIRRS------------------- 251

Query: 324 LSPLMKGQADTIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKE 383
                 GQADTIGIAMR+ LLGEIEGTCITRAKSEKIPH+YST++GIQESVH+ILMNLKE
Sbjct: 252 ------GQADTIGIAMRRVLLGEIEGTCITRAKSEKIPHDYSTLIGIQESVHKILMNLKE 311

Query: 384 IVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIIDNTQHIANLTEPINLCIELKIER 443
           IVLRSNLYGTR+ SI VKGP  VTAQDI+LPPSVEI+DNTQHIANLTEPIN  I L+I  
Sbjct: 312 IVLRSNLYGTRETSIFVKGPRYVTAQDILLPPSVEIVDNTQHIANLTEPINFYIGLQIVG 371

Query: 444 NRGYRIQTPNNFQDASYPIDAIFMPVRNANH 446
           N  YRI+T   FQD SYPIDA+FM VRN NH
Sbjct: 372 NHRYRIKTLTKFQDRSYPIDAVFMSVRNVNH 376

BLAST of Sed0026352 vs. TAIR 10
Match: ATCG00740.1 (RNA polymerase subunit alpha )

HSP 1 Score: 282.3 bits (721), Expect = 6.7e-76
Identity = 136/160 (85.00%), Postives = 148/160 (92.50%), Query Frame = 0

Query: 286 KRLYYGRFILSPLMKGQADTIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESV 345
           KRLYYGRFILSPLMKGQADTIGIAMR+ALLGEIEGTCITRAKSE IPH+YS I GIQESV
Sbjct: 25  KRLYYGRFILSPLMKGQADTIGIAMRRALLGEIEGTCITRAKSENIPHDYSNIAGIQESV 84

Query: 346 HEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIIDNTQHIANLTEPIN 405
           HEILMNL EIVLRSNLYGTR+A ICV+GPG +TA+DIILPP+VEIIDNTQHIA LTEPI+
Sbjct: 85  HEILMNLNEIVLRSNLYGTRNALICVQGPGYITARDIILPPAVEIIDNTQHIATLTEPID 144

Query: 406 LCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANH 446
           LCIELKIERNRGY ++  NNF+D SYPIDA+FMPV NANH
Sbjct: 145 LCIELKIERNRGYSLKMSNNFEDRSYPIDAVFMPVENANH 184

BLAST of Sed0026352 vs. TAIR 10
Match: ATCG00750.1 (ribosomal protein S11 )

HSP 1 Score: 197.6 bits (501), Expect = 2.2e-50
Identity = 103/118 (87.29%), Postives = 110/118 (93.22%), Query Frame = 0

Query: 164 IARELRQIGSRRNGRISSRKSTRRIPKGVIHVQASLNNTIVTVTDVRGRVISWSSAGTCG 223
           +A+ + +IGSR+N R  SRK+ RRIPKGVIHVQAS NNTIVTVTDVRGRVISWSSAGTCG
Sbjct: 1   MAKPILRIGSRKNTRSGSRKNVRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60

Query: 224 FKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGILLSF 282
           F+GTRRGTPFAAQTAAGNAIRAVVDQGMQRAEV IKGPGLGRDAALRAIRRSGILLSF
Sbjct: 61  FRGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVRIKGPGLGRDAALRAIRRSGILLSF 118

BLAST of Sed0026352 vs. TAIR 10
Match: ATCG00790.1 (ribosomal protein L16 )

HSP 1 Score: 164.9 bits (416), Expect = 1.6e-40
Identity = 74/83 (89.16%), Postives = 79/83 (95.18%), Query Frame = 0

Query: 1  MKGMSYRGNRICFGRYAIQALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVTL 60
          +KG+S RGNRICFGRYA+Q LEPAWITSRQIEAGRRAMTRN RRGGKIWVRIFPDKPVT+
Sbjct: 17 LKGISSRGNRICFGRYALQTLEPAWITSRQIEAGRRAMTRNVRRGGKIWVRIFPDKPVTV 76

Query: 61 RPTETRMGSGKGSPEYWVAVIKP 84
          RP ETRMGSGKGSPEYWVAV+KP
Sbjct: 77 RPAETRMGSGKGSPEYWVAVVKP 99

BLAST of Sed0026352 vs. TAIR 10
Match: ATCG00780.1 (ribosomal protein L14 )

HSP 1 Score: 152.5 bits (384), Expect = 8.0e-37
Identity = 80/98 (81.63%), Postives = 84/98 (85.71%), Query Frame = 0

Query: 84  DNSGARELMCIRIIGASNRRYAHIGDIIVAVIKKV----------VIRAVIVRTCKELKR 143
           DNSGARELMCIRIIGASNRRYAHIGD+IVAVIK+           VIRAVIVRTCKELKR
Sbjct: 12  DNSGARELMCIRIIGASNRRYAHIGDVIVAVIKEAIPNTPLERSEVIRAVIVRTCKELKR 71

Query: 144 ENGMIIRYDDNAAVVIDQEGNPKGTRIFGAIARELRQI 172
            NG IIRYDDNAAVVIDQEGNPKGTR+FGAI RELRQ+
Sbjct: 72  NNGTIIRYDDNAAVVIDQEGNPKGTRVFGAIPRELRQL 109

BLAST of Sed0026352 vs. TAIR 10
Match: AT2G28830.1 (PLANT U-BOX 12 )

HSP 1 Score: 115.9 bits (289), Expect = 8.3e-26
Identity = 58/82 (70.73%), Postives = 66/82 (80.49%), Query Frame = 0

Query: 2   KGMSYRGNRICFGRYAIQALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVTLR 61
           KG+S +G  IC  RYA+Q LEPAWITSRQIEAGRRAMTRN  RG  + V IF DKPVT+R
Sbjct: 847 KGVSSQG-YIC-SRYALQTLEPAWITSRQIEAGRRAMTRNIGRGLTVRVHIFADKPVTVR 906

Query: 62  PTETRMGSGKGSPEYWVAVIKP 84
           P ETRMG GKG+P +WVAV+KP
Sbjct: 907 PPETRMGRGKGAPAFWVAVVKP 926

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RDY14681.13.3e-19474.29DNA-directed RNA polymerase subunit alpha, partial [Mucuna pruriens][more]
KAD4981935.12.0e-17070.17hypothetical protein E3N88_18606 [Mikania micrantha][more]
KAF8376862.14.7e-16478.22hypothetical protein HHK36_031458 [Tetracentron sinense] >KAF8398788.1 hypotheti... [more]
KAD7117051.18.0e-16470.07hypothetical protein E3N88_04319 [Mikania micrantha][more]
KAF9660677.11.6e-15164.12hypothetical protein SADUNF_SadunfPtG0000200 [Salix dunnii] >KAF9664285.1 hypoth... [more]
Match NameE-valueIdentityDescription
Q4VZK37.7e-8596.25DNA-directed RNA polymerase subunit alpha OS=Cucumis sativus OX=3659 GN=rpoA PE=... [more]
Q09WY54.7e-8292.50DNA-directed RNA polymerase subunit alpha OS=Morus indica OX=248361 GN=rpoA PE=3... [more]
Q49KW61.5e-8091.88DNA-directed RNA polymerase subunit alpha OS=Eucalyptus globulus subsp. globulus... [more]
Q9MTJ31.7e-7990.63DNA-directed RNA polymerase subunit alpha OS=Oenothera elata subsp. hookeri OX=8... [more]
Q2PMQ31.7e-7990.00DNA-directed RNA polymerase subunit alpha OS=Glycine max OX=3847 GN=rpoA PE=3 SV... [more]
Match NameE-valueIdentityDescription
A0A371II161.6e-19474.29Plastid-encoded RNA polymerase subunit alpha (Fragment) OS=Mucuna pruriens OX=15... [more]
A0A5N6NML19.6e-17170.17S1-like domain-containing protein OS=Mikania micrantha OX=192012 GN=E3N88_18606 ... [more]
A0A5N6PW933.9e-16470.07Ribosomal_L2_C domain-containing protein OS=Mikania micrantha OX=192012 GN=E3N88... [more]
A0A5N6N9A82.6e-14468.25S1-like domain-containing protein OS=Mikania micrantha OX=192012 GN=E3N88_22057 ... [more]
A0A6A5PE044.5e-13669.82RPOLD domain-containing protein OS=Lupinus albus OX=3870 GN=Lal_00046743 PE=3 SV... [more]
Match NameE-valueIdentityDescription
ATCG00740.16.7e-7685.00RNA polymerase subunit alpha [more]
ATCG00750.12.2e-5087.29ribosomal protein S11 [more]
ATCG00790.11.6e-4089.16ribosomal protein L16 [more]
ATCG00780.18.0e-3781.63ribosomal protein L14 [more]
AT2G28830.18.3e-2670.73PLANT U-BOX 12 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000114Ribosomal protein L16PRINTSPR00060RIBOSOMALL16coord: 8..20
score: 47.85
coord: 72..101
score: 26.4
coord: 26..37
score: 71.5
coord: 42..71
score: 67.87
IPR000114Ribosomal protein L16TIGRFAMTIGR01164TIGR01164coord: 1..83
e-value: 2.6E-37
score: 125.7
IPR011263DNA-directed RNA polymerase, RpoA/D/Rpb3-typeSMARTSM00662rpoldneu2coord: 290..455
e-value: 2.2E-5
score: -5.7
IPR000218Ribosomal protein L14PSMARTSM01374Ribosomal_L14_2coord: 80..188
e-value: 8.5E-36
score: 134.9
IPR000218Ribosomal protein L14PPFAMPF00238Ribosomal_L14coord: 84..171
e-value: 9.4E-30
score: 103.1
IPR000218Ribosomal protein L14PHAMAPMF_01367Ribosomal_L14coord: 60..204
score: 18.586861
IPR011262DNA-directed RNA polymerase, insert domainPFAMPF01000RNA_pol_A_baccoord: 327..419
e-value: 1.6E-15
score: 57.5
IPR036920Ribosomal protein L10e/L16 superfamilyGENE3D3.90.1170.10Ribosomal protein L10e/L16coord: 1..82
e-value: 4.9E-38
score: 132.8
IPR036920Ribosomal protein L10e/L16 superfamilySUPERFAMILY54686Ribosomal protein L16p/L10ecoord: 1..84
IPR036853Ribosomal protein L14 superfamilyGENE3D2.40.150.20Ribosomal protein L14coord: 83..178
e-value: 3.9E-35
score: 122.4
IPR036853Ribosomal protein L14 superfamilySUPERFAMILY50193Ribosomal protein L14coord: 83..172
IPR016180Ribosomal protein L10e/L16PFAMPF00252Ribosomal_L16coord: 2..84
e-value: 1.5E-29
score: 102.7
IPR016180Ribosomal protein L10e/L16CDDcd01433Ribosomal_L16_L10ecoord: 7..83
e-value: 7.37702E-35
score: 123.756
IPR001971Ribosomal protein S11PFAMPF00411Ribosomal_S11coord: 191..280
e-value: 2.3E-35
score: 121.3
IPR001971Ribosomal protein S11HAMAPMF_01310Ribosomal_S11coord: 169..301
score: 22.598915
IPR036967Ribosomal protein S11 superfamilyGENE3D3.30.420.80Ribosomal protein S11coord: 179..296
e-value: 3.0E-32
score: 113.5
IPR036643DNA-directed RNA polymerase, insert domain superfamilyGENE3D2.170.120.12coord: 320..437
e-value: 5.8E-35
score: 121.8
IPR036643DNA-directed RNA polymerase, insert domain superfamilySUPERFAMILY56553Insert subdomain of RNA polymerase alpha subunitcoord: 320..438
NoneNo IPR availablePANTHERPTHR32108:SF1SUBFAMILY NOT NAMEDcoord: 299..445
coord: 187..290
NoneNo IPR availableCDDcd06928RNAP_alpha_NTDcoord: 286..444
e-value: 3.88025E-74
score: 230.049
NoneNo IPR availableSUPERFAMILY53137Translational machinery componentscoord: 187..279
IPR011773DNA-directed RNA polymerase, alpha subunitPANTHERPTHR32108DNA-DIRECTED RNA POLYMERASE SUBUNIT ALPHAcoord: 299..445
coord: 187..290
IPR019972Ribosomal protein L14P, conserved sitePROSITEPS00049RIBOSOMAL_L14coord: 122..148
IPR020798Ribosomal protein L16, conserved sitePROSITEPS00701RIBOSOMAL_L16_2coord: 66..77
IPR020798Ribosomal protein L16, conserved sitePROSITEPS00586RIBOSOMAL_L16_1coord: 43..54
IPR036603RNA polymerase, RBP11-like subunitSUPERFAMILY55257RBP11-like subunits of RNA polymerasecoord: 289..320

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0026352.1Sed0026352.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
biological_process GO:0006412 translation
cellular_component GO:0009507 chloroplast
cellular_component GO:0015934 large ribosomal subunit
cellular_component GO:0005840 ribosome
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0019843 rRNA binding
molecular_function GO:0003735 structural constituent of ribosome