Sed0026151 (gene) Chayote v1

Overview
NameSed0026151
Typegene
OrganismSechium edule (Chayote v1)
DescriptionrRNA N-glycosidase
LocationLG09: 4897017 .. 4897854 (+)
RNA-Seq ExpressionSed0026151
SyntenySed0026151
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATCCTCATACTTTTACTTCCTCATTGCTCTCTACCTTGGCGCTCATGTCGCCGAGGCCGATGTCTCCTTCTCGATGTTGGGTGCCACAAGCGAAACCTACAAACAGTTCATCAAAAATCTGCGTATGGCCCTTACCGTTCGATCGCAAGTGGTTACGGCATACCTGTACTGCAATCTACAGCAACAGGGCCAGCGCGCTTCAAATTAGTGCACCTCACGAATTACAATAGCGAGTCCATCTCCGTGGCGATAGACGTAGTGAACGTATATATCGTAGCATATCGAACTGGAAATACTGCCTACTTTTTGAATGATACTACAGCCGAAGCGAACAACGTTCTTTTCCGAGGCATCAACCACGTAAAGGAAATTACGACGGCCTCGAGACTGCTGCGGGGAAGATTTCGAGGGAAAAAATTGATCTGGGGTTTTCGGAGATAAGTAGTTCGATCGGCAACATGTTCCACCACAGCGTTGGGACTTCGGTTGCAAGAGCGTTCATTGTGATCATCCAAACAGTCTCGGAAGCAGCACGATTTAAATATATTGAGCAAAGGGTTTCTGAGAATGTTAAGACCAAGTTTAAGCCGGACCCTGCATTCTTAAGTTTGGAAAATCGATGGTCCGATCTCTCTGAACAAATACAAATCGCGCAAACACGAGGAGGGAAATTTGCACGTGCCGTTGAGGTTCGAAGTGTTAGCAACAAACCGATACTCGTAACTGATGTTAGTTCGCCAATTGTTAAAGGAGTTGCTCTTCTATTGTACTATAGAGTTGGTGTTGGTGTTGGTATAGAAGACATAAATGTAATGTTGATGGCAACTTACTAG

mRNA sequence

ATGAGATCCTCATACTTTTACTTCCTCATTGCTCTCTACCTTGGCGCTCATGTCGCCGAGGCCGATGTCTCCTTCTCGATGTTGGGTGCCACAAGCGAAACCTACAAACAGTTCATCAAAAATCTGCCAACAGGGCCAGCGCGCTTCAAATTAGTGCACCTCACGAATTACAATAGCGAGTCCATCTCCGTGGCGATAGACGTAGTGAACGTATATATCGTAGCATATCGAACTGGAAATACTGCCTACTTTTTGAATGATACTACAGCCGAAGCGAACAACGTTCTTTTCCGAGGCATCAACCACATTTCGAGGGAAAAAATTGATCTGGGGTTTTCGGAGATAAGTAGTTCGATCGGCAACATGTTCCACCACAGCGTTGGGACTTCGGTTGCAAGAGCGTTCATTGTGATCATCCAAACAGTCTCGGAAGCAGCACGATTTAAATATATTGAGCAAAGGGTTTCTGAGAATGTTAAGACCAAGTTTAAGCCGGACCCTGCATTCTTAAGTTTGGAAAATCGATGGTCCGATCTCTCTGAACAAATACAAATCGCGCAAACACGAGGAGGGAAATTTGCACGTGCCGTTGAGGTTCGAAGTGTTAGCAACAAACCGATACTCGTAACTGATGTTAGTTCGCCAATTGTTAAAGGAGTTGCTCTTCTATTGTACTATAGAGTTGGTGTTGGTGTTGGTATAGAAGACATAAATGTAATGTTGATGGCAACTTACTAG

Coding sequence (CDS)

ATGAGATCCTCATACTTTTACTTCCTCATTGCTCTCTACCTTGGCGCTCATGTCGCCGAGGCCGATGTCTCCTTCTCGATGTTGGGTGCCACAAGCGAAACCTACAAACAGTTCATCAAAAATCTGCCAACAGGGCCAGCGCGCTTCAAATTAGTGCACCTCACGAATTACAATAGCGAGTCCATCTCCGTGGCGATAGACGTAGTGAACGTATATATCGTAGCATATCGAACTGGAAATACTGCCTACTTTTTGAATGATACTACAGCCGAAGCGAACAACGTTCTTTTCCGAGGCATCAACCACATTTCGAGGGAAAAAATTGATCTGGGGTTTTCGGAGATAAGTAGTTCGATCGGCAACATGTTCCACCACAGCGTTGGGACTTCGGTTGCAAGAGCGTTCATTGTGATCATCCAAACAGTCTCGGAAGCAGCACGATTTAAATATATTGAGCAAAGGGTTTCTGAGAATGTTAAGACCAAGTTTAAGCCGGACCCTGCATTCTTAAGTTTGGAAAATCGATGGTCCGATCTCTCTGAACAAATACAAATCGCGCAAACACGAGGAGGGAAATTTGCACGTGCCGTTGAGGTTCGAAGTGTTAGCAACAAACCGATACTCGTAACTGATGTTAGTTCGCCAATTGTTAAAGGAGTTGCTCTTCTATTGTACTATAGAGTTGGTGTTGGTGTTGGTATAGAAGACATAAATGTAATGTTGATGGCAACTTACTAG

Protein sequence

MRSSYFYFLIALYLGAHVAEADVSFSMLGATSETYKQFIKNLPTGPARFKLVHLTNYNSESISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRGINHISREKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQTVSEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRSVSNKPILVTDVSSPIVKGVALLLYYRVGVGVGIEDINVMLMATY
Homology
BLAST of Sed0026151 vs. NCBI nr
Match: XP_022158193.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 357.8 bits (917), Expect = 7.3e-95
Identity = 201/282 (71.28%), Postives = 220/282 (78.01%), Query Frame = 0

Query: 1   MRSSYFYFLIALYLGAHVAEADVSFSMLGATSETYKQFIKNL------------------ 60
           MR   FYF +ALYLGA  AEADVSFSMLG+TSETYKQFIKN+                  
Sbjct: 1   MRLLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLK 60

Query: 61  --PTGPARFKLVHLTNYNSESISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRGI 120
              TG ARF LVHLTNYNSESI VAIDVVNVY+VAYR GN AYFLND +AEA+ VLF+GI
Sbjct: 61  ATATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGI 120

Query: 121 NH-----------------ISREKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQTVS 180
           NH                 ISREKIDLGFSEISSSIGNMFHH+ GTSV RAFIVIIQ+VS
Sbjct: 121 NHVKLSYKGNYDGLETAAKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVS 180

Query: 181 EAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRSVS 240
           EAARFKYIEQRVSENVKT FKPDPAFLSL+NRWSDLSEQIQIAQ RGG+FARAVE+R+VS
Sbjct: 181 EAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVS 240

Query: 241 NKPILVTDVSSPIVKGVALLLYYRVGVGVGIEDINVMLMATY 246
           NKPILVTDVSSP+VKG+ALLLYY+V V V  ++I  + MATY
Sbjct: 241 NKPILVTDVSSPVVKGIALLLYYKVSV-VMDDNIIKVPMATY 281

BLAST of Sed0026151 vs. NCBI nr
Match: XP_022158192.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 347.8 bits (891), Expect = 7.5e-92
Identity = 193/282 (68.44%), Postives = 217/282 (76.95%), Query Frame = 0

Query: 1   MRSSYFYFLIALYLGAHVAEADVSFSMLGATSETYKQFIKNL------------------ 60
           MR   FYF++AL  GA  AEADVSFSMLGATSETY+QFI+NL                  
Sbjct: 1   MRFLPFYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQ 60

Query: 61  --PTGPARFKLVHLTNYNSESISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRGI 120
               G ARF LVHLTNYN+ESI+VAIDVVN+Y+VAYR GN AYFLND +AEANNVLF+GI
Sbjct: 61  STAVGSARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 NH------------------ISREKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQTV 180
            H                  ISREKIDLGFSEISSSIGNMFHH+VGTS+ARAFIVIIQ+V
Sbjct: 121 KHVRLPYKGNYDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSV 180

Query: 181 SEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRSV 240
            EAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQ R G+FARA+E+R+V
Sbjct: 181 LEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTV 240

Query: 241 SNKPILVTDVSSPIVKGVALLLYYRVGVGVGIEDINVMLMAT 245
           SNKPI+VT+VSSPIVKG+ALLLYY+V V +     N++ M T
Sbjct: 241 SNKPIIVTNVSSPIVKGIALLLYYKVRVAID----NIIRMPT 278

BLAST of Sed0026151 vs. NCBI nr
Match: BAG12408.1 (karasurin-H precursor [Trichosanthes kirilowii var. japonica])

HSP 1 Score: 321.6 bits (823), Expect = 5.8e-84
Identity = 178/278 (64.03%), Postives = 210/278 (75.54%), Query Frame = 0

Query: 6   FYFLIALYLGAHVAEADVSFSMLGATSETYKQFIKNL--------PT------------G 65
           FY ++ALY+GA V EADV+FS+LG+TS TYKQFI+NL        PT            G
Sbjct: 6   FYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLKGTAAG 65

Query: 66  PARFKLVHLTNYNSESISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRGINH--- 125
            ARF LVHLTNYN ESI+VA+DVVNVY+VAYR GN AYFLND +AEANNVLF+GINH   
Sbjct: 66  LARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGINHVRL 125

Query: 126 ---------------ISREKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQTVSEAAR 185
                          ISREKI LGFSEISS+IG+MFH++ GTSV +AFIVIIQTVSEAAR
Sbjct: 126 PYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTVSEAAR 185

Query: 186 FKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRSVSNKPI 245
           FKYI+QRVSENV+TKF PDPAFLSLEN W  LSEQIQIAQ RGG+FAR +E+R+VSN P 
Sbjct: 186 FKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTVSNTPT 245

BLAST of Sed0026151 vs. NCBI nr
Match: P98184.1 (RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Bryonia dioica])

HSP 1 Score: 320.5 bits (820), Expect = 1.3e-83
Identity = 178/284 (62.68%), Postives = 209/284 (73.59%), Query Frame = 0

Query: 1   MRSSYFYFLIALYLGAHVAE-ADVSFSMLGATSETYKQFIKNLPT--------------- 60
           MRS  FY ++ALY+GAHV E  D++FS++GAT  TYK FI+NL T               
Sbjct: 1   MRSIGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVL 60

Query: 61  -----GPARFKLVHLTNYNSESISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRG 120
                G ARF+LV LTNYN ES++VA+DVVNVY+VAYR GNTAYFL D + EANNVLF G
Sbjct: 61  RNAAAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAG 120

Query: 121 INH------------------ISREKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQT 180
           INH                  ISRE I+LGFSEISS+IGNMF H+ GTSV RAFIVIIQT
Sbjct: 121 INHVRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQT 180

Query: 181 VSEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRS 240
           VSEAARFKYIEQRVSENV TKFKPDPAFLSL+N W  LSEQIQIAQTRGG+FAR VE+R+
Sbjct: 181 VSEAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRT 240

Query: 241 VSNKPILVTDVSSPIVKGVALLLYYRVGVGVGIEDINVMLMATY 246
           VSN P  VT+V+SP+VKG+ALLLY+RV VG   +++  M ++TY
Sbjct: 241 VSNTPTFVTNVNSPVVKGIALLLYFRVNVGT--DNVFAMSLSTY 282

BLAST of Sed0026151 vs. NCBI nr
Match: XP_022158191.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 275.8 bits (704), Expect = 3.6e-70
Identity = 149/243 (61.32%), Postives = 180/243 (74.07%), Query Frame = 0

Query: 21  ADVSFSMLGATSETYKQFIKNL--------------------PTGPARFKLVHLTNYNSE 80
           ++VSFS+LGAT +TY+QFI+NL                        ARF LVHLTNY +E
Sbjct: 35  SNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNE 94

Query: 81  SISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRGINH------------------ 140
           +I+VAIDVVNVYIVAY+ GN AYFL D + EA +VLF+GI                    
Sbjct: 95  TITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGK 154

Query: 141 ISREKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQTVSEAARFKYIEQRVSENVKTK 200
           ISREKIDLGFSE+ S+IGNM+H++ GTSV RAFIV+IQT+SEAARFKYIE +VS+NV+TK
Sbjct: 155 ISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETK 214

Query: 201 FKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRSVSNKPILVTDVSSPIVKGVAL 226
           FKPDP FLSLENRWSDLSEQ+QIAQ R G+FAR +EVRSV+NKPILVT+V S +V+G+AL
Sbjct: 215 FKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMAL 274

BLAST of Sed0026151 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 1.7e-86
Identity = 178/284 (62.68%), Postives = 209/284 (73.59%), Query Frame = 0

Query: 1   MRSSYFYFLIALYLGAHVAE-ADVSFSMLGATSETYKQFIKNLPT--------------- 60
           MRS  FY ++ALY+GAHV E  D++FS++GAT  TYK FI+NL T               
Sbjct: 1   MRSIGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVL 60

Query: 61  -----GPARFKLVHLTNYNSESISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRG 120
                G ARF+LV LTNYN ES++VA+DVVNVY+VAYR GNTAYFL D + EANNVLF G
Sbjct: 61  RNAAAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAG 120

Query: 121 INH------------------ISREKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQT 180
           INH                  ISRE I+LGFSEISS+IGNMF H+ GTSV RAFIVIIQT
Sbjct: 121 INHVRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQT 180

Query: 181 VSEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRS 240
           VSEAARFKYIEQRVSENV TKFKPDPAFLSL+N W  LSEQIQIAQTRGG+FAR VE+R+
Sbjct: 181 VSEAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRT 240

Query: 241 VSNKPILVTDVSSPIVKGVALLLYYRVGVGVGIEDINVMLMATY 246
           VSN P  VT+V+SP+VKG+ALLLY+RV VG   +++  M ++TY
Sbjct: 241 VSNTPTFVTNVNSPVVKGIALLLYFRVNVGT--DNVFAMSLSTY 282

BLAST of Sed0026151 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 172.2 bits (435), Expect = 7.4e-42
Identity = 106/258 (41.09%), Postives = 157/258 (60.85%), Query Frame = 0

Query: 7   YFLIALYLGAHVAEADVSFSMLGATSETYKQFIKN----LP---------------TGPA 66
           + +IA+++G   A+ DV+F +  AT++TY +FI++    LP               +   
Sbjct: 9   FLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLLYSTISDSR 68

Query: 67  RFKLVHLTNYNSESISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRGINHIS--- 126
           RF L++LT+Y  E+ISVAIDV NVY+VAYRT + +YF  ++  EA N+LF+G   I+   
Sbjct: 69  RFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPY 128

Query: 127 --------------REKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQTVSEAARFKY 186
                         RE IDLG   +SS+I  +F+++   S   A +V+IQT +EAARFKY
Sbjct: 129 TGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYN-AQSAPSALLVLIQTTAEAARFKY 188

Query: 187 IEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRSVSNKPILVT 228
           IE+ V++ V T FKP+ A +SLEN+WS LS+QI +AQ +GGKF   V++   + +   VT
Sbjct: 189 IERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVT 248

BLAST of Sed0026151 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.3e-41
Identity = 106/258 (41.09%), Postives = 156/258 (60.47%), Query Frame = 0

Query: 7   YFLIALYLGAHVAEADVSFSMLGATSETYKQFIKN----LP---------------TGPA 66
           + +IA+++G   A+ DV+F +  AT++TY +FI++    LP               +   
Sbjct: 9   FLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLLYSTISDSR 68

Query: 67  RFKLVHLTNYNSESISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRGINHIS--- 126
           RF L+ LT+Y  E+ISVAIDV NVY+VAYRT + +YF  ++  EA N+LF+G   I+   
Sbjct: 69  RFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPY 128

Query: 127 --------------REKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQTVSEAARFKY 186
                         RE IDLG   +SS+I  +F+++   S   A +V+IQT +EAARFKY
Sbjct: 129 TGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYN-AQSAPSALLVLIQTTAEAARFKY 188

Query: 187 IEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRSVSNKPILVT 228
           IE+ V++ V T FKP+ A +SLEN+WS LS+QI +AQ +GGKF   V++   + +   VT
Sbjct: 189 IERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVT 248

BLAST of Sed0026151 vs. ExPASy Swiss-Prot
Match: P56626 (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=50544 GN=TCA PE=1 SV=2)

HSP 1 Score: 164.5 bits (415), Expect = 1.5e-39
Identity = 104/263 (39.54%), Postives = 148/263 (56.27%), Query Frame = 0

Query: 7   YFLIALYLGAHVAEADVSFSMLGATSETYKQFIKN----LPT---------------GPA 66
           +F +A+ LG+  A  DVSF +  AT ++Y  FI      LPT               G  
Sbjct: 5   FFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTASGSQ 64

Query: 67  RFKLVHLTNYNSESISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRGINHI---- 126
            F+  +LTNYN E+++VA++V NVYIVAYR    +YF  DT AEA  ++F G   +    
Sbjct: 65  WFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTVKLPY 124

Query: 127 -------------SREKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQTVSEAARFKY 186
                         R+ I+LG   +SS+I NM ++    S A A +V+IQ  +EAAR+KY
Sbjct: 125 SGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDY-QSTAAALLVLIQCTAEAARYKY 184

Query: 187 IEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQTRG-GKFARAVEVRSVSNKPILV 232
           IEQ+VS ++ + F P+ A +SLEN+W  LS+QIQIA   G G+F   VE+ +       V
Sbjct: 185 IEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTRFSV 244

BLAST of Sed0026151 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 5.9e-39
Identity = 100/268 (37.31%), Postives = 156/268 (58.21%), Query Frame = 0

Query: 9   LIALYLGAHVAEADVSFSMLGATSETYKQFI----KNLP---------------TGPARF 68
           ++A+++ A   EADV FS+ G++S +Y +FI    K LP               +G +R+
Sbjct: 8   ILAIFVAASTVEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSSASGASRY 67

Query: 69  KLVHLTNYNSESISVAIDVVNVYIVAYRTGNTAYFLNDTTAE-ANNVLFRGINHIS---- 128
            L+ L+NY+ ++I+VA+DV NVYI+ Y   +T+YF N++ A+ A+  +F+G   ++    
Sbjct: 68  TLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYS 127

Query: 129 -------------REKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQTVSEAARFKYI 188
                        REKI LGF  + S+I  +FH+   T+ A AF+VIIQT +EA+RFKYI
Sbjct: 128 GNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYD-STAAAAAFLVIIQTTAEASRFKYI 187

Query: 189 EQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRSVSNKPILVTD 239
           E ++ E +     P  A +SLEN WS LS+QIQ+AQT  G F   V +     + + +T+
Sbjct: 188 EGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRVEITN 247

BLAST of Sed0026151 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 3.5e-95
Identity = 201/282 (71.28%), Postives = 220/282 (78.01%), Query Frame = 0

Query: 1   MRSSYFYFLIALYLGAHVAEADVSFSMLGATSETYKQFIKNL------------------ 60
           MR   FYF +ALYLGA  AEADVSFSMLG+TSETYKQFIKN+                  
Sbjct: 1   MRLLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLK 60

Query: 61  --PTGPARFKLVHLTNYNSESISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRGI 120
              TG ARF LVHLTNYNSESI VAIDVVNVY+VAYR GN AYFLND +AEA+ VLF+GI
Sbjct: 61  ATATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGI 120

Query: 121 NH-----------------ISREKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQTVS 180
           NH                 ISREKIDLGFSEISSSIGNMFHH+ GTSV RAFIVIIQ+VS
Sbjct: 121 NHVKLSYKGNYDGLETAAKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVS 180

Query: 181 EAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRSVS 240
           EAARFKYIEQRVSENVKT FKPDPAFLSL+NRWSDLSEQIQIAQ RGG+FARAVE+R+VS
Sbjct: 181 EAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVS 240

Query: 241 NKPILVTDVSSPIVKGVALLLYYRVGVGVGIEDINVMLMATY 246
           NKPILVTDVSSP+VKG+ALLLYY+V V V  ++I  + MATY
Sbjct: 241 NKPILVTDVSSPVVKGIALLLYYKVSV-VMDDNIIKVPMATY 281

BLAST of Sed0026151 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.6e-92
Identity = 193/282 (68.44%), Postives = 217/282 (76.95%), Query Frame = 0

Query: 1   MRSSYFYFLIALYLGAHVAEADVSFSMLGATSETYKQFIKNL------------------ 60
           MR   FYF++AL  GA  AEADVSFSMLGATSETY+QFI+NL                  
Sbjct: 1   MRFLPFYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQ 60

Query: 61  --PTGPARFKLVHLTNYNSESISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRGI 120
               G ARF LVHLTNYN+ESI+VAIDVVN+Y+VAYR GN AYFLND +AEANNVLF+GI
Sbjct: 61  STAVGSARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 NH------------------ISREKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQTV 180
            H                  ISREKIDLGFSEISSSIGNMFHH+VGTS+ARAFIVIIQ+V
Sbjct: 121 KHVRLPYKGNYDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSV 180

Query: 181 SEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRSV 240
            EAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQ R G+FARA+E+R+V
Sbjct: 181 LEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTV 240

Query: 241 SNKPILVTDVSSPIVKGVALLLYYRVGVGVGIEDINVMLMAT 245
           SNKPI+VT+VSSPIVKG+ALLLYY+V V +     N++ M T
Sbjct: 241 SNKPIIVTNVSSPIVKGIALLLYYKVRVAID----NIIRMPT 278

BLAST of Sed0026151 vs. ExPASy TrEMBL
Match: B1B626 (rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.8e-84
Identity = 178/278 (64.03%), Postives = 210/278 (75.54%), Query Frame = 0

Query: 6   FYFLIALYLGAHVAEADVSFSMLGATSETYKQFIKNL--------PT------------G 65
           FY ++ALY+GA V EADV+FS+LG+TS TYKQFI+NL        PT            G
Sbjct: 6   FYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLKGTAAG 65

Query: 66  PARFKLVHLTNYNSESISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRGINH--- 125
            ARF LVHLTNYN ESI+VA+DVVNVY+VAYR GN AYFLND +AEANNVLF+GINH   
Sbjct: 66  LARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGINHVRL 125

Query: 126 ---------------ISREKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQTVSEAAR 185
                          ISREKI LGFSEISS+IG+MFH++ GTSV +AFIVIIQTVSEAAR
Sbjct: 126 PYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTVSEAAR 185

Query: 186 FKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRSVSNKPI 245
           FKYI+QRVSENV+TKF PDPAFLSLEN W  LSEQIQIAQ RGG+FAR +E+R+VSN P 
Sbjct: 186 FKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTVSNTPT 245

BLAST of Sed0026151 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.8e-70
Identity = 149/243 (61.32%), Postives = 180/243 (74.07%), Query Frame = 0

Query: 21  ADVSFSMLGATSETYKQFIKNL--------------------PTGPARFKLVHLTNYNSE 80
           ++VSFS+LGAT +TY+QFI+NL                        ARF LVHLTNY +E
Sbjct: 35  SNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNE 94

Query: 81  SISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRGINH------------------ 140
           +I+VAIDVVNVYIVAY+ GN AYFL D + EA +VLF+GI                    
Sbjct: 95  TITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGK 154

Query: 141 ISREKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQTVSEAARFKYIEQRVSENVKTK 200
           ISREKIDLGFSE+ S+IGNM+H++ GTSV RAFIV+IQT+SEAARFKYIE +VS+NV+TK
Sbjct: 155 ISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETK 214

Query: 201 FKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRSVSNKPILVTDVSSPIVKGVAL 226
           FKPDP FLSLENRWSDLSEQ+QIAQ R G+FAR +EVRSV+NKPILVT+V S +V+G+AL
Sbjct: 215 FKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMAL 274

BLAST of Sed0026151 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 2.6e-66
Identity = 149/265 (56.23%), Postives = 183/265 (69.06%), Query Frame = 0

Query: 12  LYLGAHV--------AEADVSFSMLGATSETYKQFIKNL--------------------P 71
           L  GAHV        A A++SFS+LGAT++TYKQFI +L                     
Sbjct: 17  LNCGAHVAIRAPRPSANANLSFSLLGATAQTYKQFISDLRKALTVNSHKVYDIPVLPATA 76

Query: 72  TGPARFKLVHLTNYNSESISVAIDVVNVYIVAYRTGNTAYFLNDTTAEANNVLFRGINH- 131
            G ARF LV+LTNY  ESI+VAID V+VYIVAY+ GN AYFL D + EA++VLF+G  H 
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 132 -----------------ISREKIDLGFSEISSSIGNMFHHSVGTSVARAFIVIIQTVSEA 191
                            ISRE IDLGFSE+SSSIGNMFH++ G SV ++FIV+IQ +SEA
Sbjct: 137 TLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAGYSVPKSFIVMIQMLSEA 196

Query: 192 ARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQTRGGKFARAVEVRSVSNK 231
           +RFKYIE R  ENV++KFKPDP +LSLENRWS+LSEQ+Q AQ  GGKF   V+VRSVSNK
Sbjct: 197 SRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNK 256

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158193.17.3e-9571.28ribosome-inactivating protein bryodin II-like [Momordica charantia][more]
XP_022158192.17.5e-9268.44ribosome-inactivating protein bryodin II-like [Momordica charantia][more]
BAG12408.15.8e-8464.03karasurin-H precursor [Trichosanthes kirilowii var. japonica][more]
P98184.11.3e-8362.68RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltNa... [more]
XP_022158191.13.6e-7061.32ribosome-inactivating protein bryodin II-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
P981841.7e-8662.68Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1[more]
P248177.4e-4241.09Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN... [more]
P293391.3e-4141.09Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV... [more]
P566261.5e-3939.54Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=5... [more]
Q004655.9e-3937.31Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DVE73.5e-9571.28rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1[more]
A0A6J1DWK43.6e-9268.44rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1[more]
B1B6262.8e-8464.03rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1[more]
A0A6J1E0931.8e-7061.32rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1[more]
A0A6J1IUB22.6e-6656.23rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016139Ribosome-inactivating protein, subdomain 2GENE3D4.10.470.10Ricin (A Subunit), domain 2coord: 148..230
e-value: 1.9E-26
score: 93.7
IPR016138Ribosome-inactivating protein, subdomain 1GENE3D3.40.420.10Ricin (A subunit), domain 1coord: 102..147
e-value: 3.8E-8
score: 35.2
IPR016138Ribosome-inactivating protein, subdomain 1GENE3D3.40.420.10Ricin (A subunit), domain 1coord: 24..101
e-value: 1.6E-11
score: 46.1
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 43..184
e-value: 2.1E-18
score: 66.7
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 42..225
NoneNo IPR availablePANTHERPTHR33453:SF14RRNA N-GLYCOSIDASEcoord: 42..225
IPR017988Ribosome-inactivating protein conserved sitePROSITEPS00275SHIGA_RICINcoord: 139..155
IPR036041Ribosome-inactivating protein superfamilySUPERFAMILY56371Ribosome inactivating proteins (RIP)coord: 22..229

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0026151.1Sed0026151.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0017148 negative regulation of translation
molecular_function GO:0030598 rRNA N-glycosylase activity