Sed0026127 (gene) Chayote v1

Overview
NameSed0026127
Typegene
OrganismSechium edule (Chayote v1)
DescriptionDNA-directed RNA polymerase II subunit 4
LocationLG03: 1976697 .. 1980395 (-)
RNA-Seq ExpressionSed0026127
SyntenySed0026127
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTAAAATAAAACTGTACAAAATGATATTTCAGCAAAAAATATTAGTGAAAGCTCTGTATTACACCTTCTCTGTTTTCGAGCGACTCGGCGCTGGATCTGGCCTCTAATCCTCTCTGTGTTTTGTGGTGCGTTCTCCTTCAGTTTTTTTTCTTTTCGTTCACAAACTGGTTCTACAATTTGATTATGGATAGAACGGATTGGCTTGATTGCCGTTTTTGCTGTGTATATTCTTCTAATTTCAGACTCGAGGCATTGAATTCGGTTGGAATTTGATATTCATTTCCGTTCTTGAATAAGAATATTCTCTTCGTTGCAAGATTTTCATAATTTGGTATAAATCCATTTAGTTAGGGTTCAAATGCCCTCTTTGTTCCCATTTTGTTAGGGTTCATTTGGGCTCATTTTAATATGAATAAGTTGAATGAATTTTATTGTGTCTAGACTCGCAGAGTATGTTACCAGGTTGATGGAAAATGTTTTGGTTTTTTTACATTGAGATTATGGAGCATACCAGTGCTTTAATGGATTCTATGAGTTGCATCAGGAGGACAATTTCTTAATGTTATACTCTAAAAACCATTAAAAGGAAATGTGAATGGAAGAATCTTTTTGTGTCTTCTGTCTGAAGGCTCTCAATTTCAATGTAGCTAGTGTTCTCGAGGGATGGCACGGTGGTTGAAGGCTTGAGTTTTGAAGGTGTGTTCCTTTCAAGATCTTAGATTCGAGAATCATGTGACATTACCCGCGACTCCATTCTTTGATGCCTCTTGGACCCTGGCACGTGGCCTAGGGACGGGCGTGGTTACCGTAGATATAATGAAGCAAAGCTCCACTTTCCCGATTTCCTTTAATTTTTTTTTTTTTTTAAATGTAGTTAGTGTTAACTTTGATTTTTCCCTTCTTTTACTTTACTCTTGATGCCTTGCAATTTGTTAGGAATTTTGTCCATATTTTGTAGATCCAGTTTTGATATTGCTGTACTCTTTTCAGATTCTGTAGGCTGTCCAAAACTCCAACTTCAAGCATGTCTGGAGAGGAGGAAGAGAATGCCGCAGAGCTTAAAATTGGGGATGGTACCAATCTATCTATAAATCTCTACTTTTTCATCAACAATCAACGAGATTTTAAGTCTGCTCTTACTGTTTATGGTGATGGGTTTCCTTCATGTTCCCCATATCCCTTATAACTAATTATATGTCTTCTTATTACACAGTTTTTCTAACAGTAACACTGTAACAGTGAATATAGTTTGGACTGTGAATGTCGAGTTAAAGTAGACACCATTTAATTTTGTAGCAAAAAATTTACAATCTCTTATATTAAATCGCGTACCCCGTGGATGGTTATATATCTTTGTTGTGAGAGTTGAAAGATAGTAGATTCCTTGTTTATCTATTTCATTAGTTTATGTGATTATGTCTTGTAGCCATGTACCTAGGATCATAGTGTTTCATTACTTTCACATATATTCTTAGATAAAAAATAGTCGTTAAATGTAGTGTCTTATAGGTTGTTGGGCATCCACATTAATAAAAAGAAGTCTCTTTTGTGTACATCTTATGGTTGTTTTTATTATGATATTGTGATAAGGCTCAAATAGCTAAATGCCTTGACTTTTCTGATAGTTTGGATTAATATTATATGGTCTTCAGGTTTCTGAAGTTTGTTTTTTTTTTTGGACAAGAGCTTTATGAAGTTTTAGATGAAGATATGTGAACGATGCAAAATCCAAATTTTAATATATAACATTTTGTCAAAATGAAACTTAACTATTGTAAGTTCTTCCACTTTTTATGAATCTATGATATTTAAGGCTTTAGAATACATCTTTGGTTTTTCCACTTCAAAATTTATGCTCTTGCCTGGCACCGAAATCTTTTTGGCGCATTCACTAAACTTTTTCATTCTCTTGTATTTATTTCACGCTTTGTTCTGAAGTGCATAAAAGAAAACATTAAAGGTGACAACTGACATTTATTTCCTTTGGCCGCTCGCAGAGTTCTTAAAAGCTAAGTGTTTAATGAATTGTGAAGTTTCATTGATACTTGAACACAAGTATGAACAACTTCAGCAGATGTCTGAAGATCCAACGAATCAAGTTTCTCAGTGAGTTTTTCTTATCCCAAAAGAAAAAACTTTCTCGATGAGTTTTATGTTTATTCTTTATGGATTCAAAAACTAATTTTGAGTTTCTTCTGCTGGTTGAGACAACTAATATATAAACGCATTAGTTACGATATTCATTATGACTTCTCATTACATTTCTGGTCTTCTTTCTGCAGAGTGTTTGAAAAGTCACTGCAGTATGTGAAACGTTTCAGTCGCTATAAAAATCCTGATGCTGTTAGACAAGTTCGAGAGTATCCTTTAAATTATACACTTTAGTTTTTATTTTATGGGATGGTGGGAAGAATTGGTTGCACATGTCCACCCTATGCTATAACCAGCTACTTTTTTTTAATGCAACAACAAGAGATTAGAGGATTTGAACCTATGACATCATGATCGGTCAGCAAGGCTAAGCCGCTTTGGCATAACCGACTACAGTTTTGAACTTGTACCTTACCATTTTCTTGCTAGCCAAGAACTCTTTGGTTAATTATTATTTAGGAACTAGTGAATAAATTAAAATTCAGTACTTTGGTACTTAATTCATGGAGGGCAATGCAGTTTACACAAATCACCTCAGGAATAAGCTTAGTTTAGGTTCATTGTTCGCATAAACTAGAGGTGTCGGGTGTGTAATTAAAATCTTAACTACCATACAGAATTCTAAGCAGGTATCAGTTGGCTGAGTTTGAGGTATATACCCGACTCTTGGTTTAACCAATTTACAGATCATTATTATCTCTTTTCTTCATTAGGAAACATTTATCTCATCACTCTTTAAATTTGTAGCTCTGTGTTCTTGGCAACCTTTGTCCTGAAACTGTGGAGGAAGCTATTGCTATGGTGCCATCTTTGAAGGTATCTTTTGCCGCCTTTTTATGCCTACATAATGTCAACAAGTATAGTAGTTTTTTTGTTTCTTGTAATTGGTAGTATATTTATGTCGCCATGGTCAAAGACTTGAGTTTTGAAGGTGTGCTCTCTAGGTCTCAGATTTGAGACTCACCCGTGATATTACTTTATAGACACTTTTCGTGTTTCCACTCCTTCAATGTCTCCCGGTGCTTAGTCTAGGGACAGACGTGGTTATCTCGGGTATAGTTGAGCGAAGTTTTGATTTTCCAGTTTCCAAAAAGAAAAATGGTAGTATATTTATAGGTATTTTGCTAATCATAGGGTCCCGACTTTTTGCAAGTTTACAGTTTGTTTAATTTTCAACATTGAACGATGTCATAGTTTTAAAGTACTATTAAAAGTTATAACATTAATATGTAATTCCAAATGGAGTAGAATTGCTTATGGATGCTTTTGTTGGTTTCAGACTAAAGGGAGAGCGCATGACGATGAAGCAATTGAGAAGATGTTGAACGATCTATCGTTAATTAAAAAGTTTGACTAGTCACTTTCTAAATCTAGGTTAAATATGTTTAAATCTATTTACTATCATTTTGTCACCTTACAATGGTTGTACCCATCTGTTGAGGCATATTATAATTTGATGGGTTTGTCATAGTATTATAGTTTCATGGGTAAGTTTGTCACCAAGTTTATCTCAATTTAAATTTGAGCTATATATTTTTCTTTTTTCA

mRNA sequence

ATTAAAATAAAACTGTACAAAATGATATTTCAGCAAAAAATATTAGTGAAAGCTCTGTATTACACCTTCTCTGTTTTCGAGCGACTCGGCGCTGGATCTGGCCTCTAATCCTCTCTGTGTTTTGTGCTAGTGTTCTCGAGGGATGGCACGGTGGTTGAAGGCTTGAGTTTTGAAGATTCTGTAGGCTGTCCAAAACTCCAACTTCAAGCATGTCTGGAGAGGAGGAAGAGAATGCCGCAGAGCTTAAAATTGGGGATGAGTTCTTAAAAGCTAAGTGTTTAATGAATTGTGAAGTTTCATTGATACTTGAACACAAGTATGAACAACTTCAGCAGATGTCTGAAGATCCAACGAATCAAGTTTCTCAAGTGTTTGAAAAGTCACTGCAGTATGTGAAACGTTTCAGTCGCTATAAAAATCCTGATGCTGTTAGACAAGTTCGAGAAATTCTAAGCAGGTATCAGTTGGCTGAGTTTGAGCTCTGTGTTCTTGGCAACCTTTGTCCTGAAACTGTGGAGGAAGCTATTGCTATGGTGCCATCTTTGAAGACTAAAGGGAGAGCGCATGACGATGAAGCAATTGAGAAGATGTTGAACGATCTATCGTTAATTAAAAAGTTTGACTAGTCACTTTCTAAATCTAGGTTAAATATGTTTAAATCTATTTACTATCATTTTGTCACCTTACAATGGTTGTACCCATCTGTTGAGGCATATTATAATTTGATGGGTTTGTCATAGTATTATAGTTTCATGGGTAAGTTTGTCACCAAGTTTATCTCAATTTAAATTTGAGCTATATATTTTTCTTTTTTCA

Coding sequence (CDS)

ATGTCTGGAGAGGAGGAAGAGAATGCCGCAGAGCTTAAAATTGGGGATGAGTTCTTAAAAGCTAAGTGTTTAATGAATTGTGAAGTTTCATTGATACTTGAACACAAGTATGAACAACTTCAGCAGATGTCTGAAGATCCAACGAATCAAGTTTCTCAAGTGTTTGAAAAGTCACTGCAGTATGTGAAACGTTTCAGTCGCTATAAAAATCCTGATGCTGTTAGACAAGTTCGAGAAATTCTAAGCAGGTATCAGTTGGCTGAGTTTGAGCTCTGTGTTCTTGGCAACCTTTGTCCTGAAACTGTGGAGGAAGCTATTGCTATGGTGCCATCTTTGAAGACTAAAGGGAGAGCGCATGACGATGAAGCAATTGAGAAGATGTTGAACGATCTATCGTTAATTAAAAAGTTTGACTAG

Protein sequence

MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKKFD
Homology
[Close]
BLAST of Sed0026127 vs. NCBI nr
Match: XP_022939401.1 (DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022939402.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022993195.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita maxima] >XP_022993196.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita maxima] >XP_023549816.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita pepo subsp. pepo] >XP_023549818.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita pepo subsp. pepo] >KAG6578503.1 DNA-directed RNA polymerase II subunit 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016067.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 268.9 bits (686), Expect = 2.5e-68
Identity = 136/138 (98.55%), Postives = 138/138 (100.00%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFD 139
           DEAIEKMLNDLSLIKKF+
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of Sed0026127 vs. NCBI nr
Match: XP_022133507.1 (DNA-directed RNA polymerase II subunit 4 [Momordica charantia])

HSP 1 Score: 267.7 bits (683), Expect = 5.6e-68
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFD 139
           DEAIEKMLNDLSLIKKF+
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of Sed0026127 vs. NCBI nr
Match: XP_038884885.1 (DNA-directed RNA polymerase II subunit 4 [Benincasa hispida])

HSP 1 Score: 267.3 bits (682), Expect = 7.3e-68
Identity = 135/138 (97.83%), Postives = 137/138 (99.28%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFD 139
           DEAIEKMLNDLSLIK+F+
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]
BLAST of Sed0026127 vs. NCBI nr
Match: XP_004138390.1 (DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >XP_008456795.1 PREDICTED: DNA-directed RNA polymerase II subunit 4 [Cucumis melo] >XP_011656444.1 DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >KAA0051957.1 DNA-directed RNA polymerase II subunit 4 [Cucumis melo var. makuwa] >KGN45845.1 hypothetical protein Csa_005663 [Cucumis sativus] >TYK04594.1 DNA-directed RNA polymerase II subunit 4 [Cucumis melo var. makuwa])

HSP 1 Score: 266.9 bits (681), Expect = 9.5e-68
Identity = 134/138 (97.10%), Postives = 137/138 (99.28%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFD 139
           DEAIEKMLNDLSLIK+F+
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]
BLAST of Sed0026127 vs. NCBI nr
Match: XP_030924038.1 (DNA-directed RNA polymerase II subunit 4 [Quercus lobata] >KAB1210402.1 DNA-directed RNA polymerase II subunit 4 [Morella rubra] >KAF3942865.1 hypothetical protein CMV_030520 [Castanea mollissima])

HSP 1 Score: 265.8 bits (678), Expect = 2.1e-67
Identity = 134/138 (97.10%), Postives = 137/138 (99.28%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEV+LILEHKYEQLQQMSEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPS+KTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSIKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFD 139
           DEAIEKMLNDLSLIKKF+
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of Sed0026127 vs. ExPASy Swiss-Prot
Match: O48890 (DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB4 PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 2.6e-68
Identity = 129/138 (93.48%), Postives = 136/138 (98.55%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ+SEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSR+QL EFELCVLGNLCPETVEEA+AMVPSLKTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFD 139
           DEAIEKMLNDLSL+K+F+
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

[Close]
BLAST of Sed0026127 vs. ExPASy Swiss-Prot
Match: O15514 (DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 3.1e-21
Identity = 56/136 (41.18%), Postives = 92/136 (67.65%), Query Frame = 0 

Query: 2   SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q +  S +   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNE--SAEDEQELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHDD 121
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ A++PSL  +GR  +D
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGR-FED 129

Query: 122 EAIEKMLNDLSLIKKF 138
           E ++++L+D+   + F
Sbjct: 130 EELQQILDDIQTKRSF 140

[Close]
BLAST of Sed0026127 vs. ExPASy Swiss-Prot
Match: Q9D7M8 (DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d PE=1 SV=2)

HSP 1 Score: 102.8 bits (255), Expect = 3.1e-21
Identity = 56/136 (41.18%), Postives = 92/136 (67.65%), Query Frame = 0 

Query: 2   SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q +  S +   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNE--SAEDEQELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHDD 121
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ A++PSL  +GR  +D
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGR-FED 129

Query: 122 EAIEKMLNDLSLIKKF 138
           E ++++L+D+   + F
Sbjct: 130 EELQQILDDIQTKRSF 140

[Close]
BLAST of Sed0026127 vs. ExPASy Swiss-Prot
Match: Q54S04 (DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689 GN=polr2d PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 1.9e-18
Identity = 54/148 (36.49%), Postives = 89/148 (60.14%), Query Frame = 0 

Query: 4   EEEENAAELKIGDEFLKAKCLMNCEVSLILEHK----------YEQ---LQQMSEDPTNQ 63
           +EEE+   LK   +   AK L+N EV+++LEH+          + Q   L  +  +  N 
Sbjct: 12  QEEEDLTTLKFPRDLKDAKFLLNSEVAILLEHRKGISESEGTEFPQNTLLIHLYYNSIND 71

Query: 64  VSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVP 123
           + + F K+L Y ++FSRYKN  +++QVR  LS+  L EFE+  L NLCPE  +EA +++P
Sbjct: 72  IIRTFHKTLAYAEKFSRYKNKTSIKQVRTALSKQNLEEFEIASLANLCPEISDEAKSLIP 131

Query: 124 SLKTKGRAHDDEAIEKMLNDLSLIKKFD 139
           SLK      +D+ ++ +L++LS ++KF+
Sbjct: 132 SLKKM----EDDELQAILDELSNLRKFN 155

[Close]
BLAST of Sed0026127 vs. ExPASy Swiss-Prot
Match: Q9VEA5 (DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 GN=Rpb4 PE=2 SV=5)

HSP 1 Score: 87.8 bits (216), Expect = 1.0e-16
Identity = 49/126 (38.89%), Postives = 80/126 (63.49%), Query Frame = 0 

Query: 6   EENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQYVKRF 65
           +E+AA+L+   EF  A+ L+  EV ++L+H+  + Q  S D   + S+VF K+  Y   F
Sbjct: 11  DEDAADLQFPKEFENAETLLISEVHMLLDHR--KRQNESADEEQEFSEVFMKTYAYTDSF 70

Query: 66  SRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHDDEAIE 125
            ++KN + +   R +L + +L +FEL  LGNLCPE  EEA A++PSL  +GR  +DE + 
Sbjct: 71  RKFKNKETIMSARSLLMQKKLHKFELAALGNLCPEAPEEAKALIPSL--EGR-FEDEELR 130

Query: 126 KMLNDL 132
           ++L+D+
Sbjct: 131 QILDDI 131

[Close]
BLAST of Sed0026127 vs. ExPASy TrEMBL
Match: A0A6J1JVN3 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489290 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.2e-68
Identity = 136/138 (98.55%), Postives = 138/138 (100.00%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFD 139
           DEAIEKMLNDLSLIKKF+
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of Sed0026127 vs. ExPASy TrEMBL
Match: A0A6J1FFS7 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita moschata OX=3662 GN=LOC111445330 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.2e-68
Identity = 136/138 (98.55%), Postives = 138/138 (100.00%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFD 139
           DEAIEKMLNDLSLIKKF+
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of Sed0026127 vs. ExPASy TrEMBL
Match: A0A6J1BWW4 (DNA-directed RNA polymerase II subunit 4 OS=Momordica charantia OX=3673 GN=LOC111006069 PE=4 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 2.7e-68
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFD 139
           DEAIEKMLNDLSLIKKF+
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of Sed0026127 vs. ExPASy TrEMBL
Match: A0A5D3C019 (DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G002310 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.6e-68
Identity = 134/138 (97.10%), Postives = 137/138 (99.28%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFD 139
           DEAIEKMLNDLSLIK+F+
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]
BLAST of Sed0026127 vs. ExPASy TrEMBL
Match: A0A0A0K7V5 (RPOL4c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014650 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.6e-68
Identity = 134/138 (97.10%), Postives = 137/138 (99.28%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFD 139
           DEAIEKMLNDLSLIK+F+
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]
BLAST of Sed0026127 vs. TAIR 10
Match: AT5G09920.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 259.2 bits (661), Expect = 1.8e-69
Identity = 129/138 (93.48%), Postives = 136/138 (98.55%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ+SEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSR+QL EFELCVLGNLCPETVEEA+AMVPSLKTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFD 139
           DEAIEKMLNDLSL+K+F+
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

[Close]
BLAST of Sed0026127 vs. TAIR 10
Match: AT4G15950.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 84.3 bits (207), Expect = 8.1e-17
Identity = 44/116 (37.93%), Postives = 75/116 (64.66%), Query Frame = 0 

Query: 21  AKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREI 80
           A+C+M+CE   IL+    QL  +SEDP+ ++   ++++L YV+    Y NP +VR+V E 
Sbjct: 89  AECMMDCEAFQILDGIKGQLVGLSEDPSIKIPVSYDRALAYVESCVHYTNPQSVRKVLEP 148

Query: 81  LSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKK 137
           L  Y +++ E+CV+ N   E+V+E +A +PSLKTK +   ++ ++  L +LS +KK
Sbjct: 149 LKTYGISDGEMCVIANASSESVDEVLAFIPSLKTK-KEVINQPLQDALEELSKLKK 203

The following BLAST results are available for this feature:
BLAST of Sed0026127 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022939401.12.5e-6898.55DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022939402... [more]
XP_022133507.15.6e-6898.55DNA-directed RNA polymerase II subunit 4 [Momordica charantia][more]
XP_038884885.17.3e-6897.83DNA-directed RNA polymerase II subunit 4 [Benincasa hispida][more]
XP_004138390.19.5e-6897.10DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >XP_008456795.1 PREDI... [more]
XP_030924038.12.1e-6797.10DNA-directed RNA polymerase II subunit 4 [Quercus lobata] >KAB1210402.1 DNA-dire... [more]
BLAST of Sed0026127 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O488902.6e-6893.48DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB... [more]
O155143.1e-2141.18DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE... [more]
Q9D7M83.1e-2141.18DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d P... [more]
Q54S041.9e-1836.49DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689... [more]
Q9VEA51.0e-1638.89DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 G... [more]
BLAST of Sed0026127 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JVN31.2e-6898.55DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1FFS71.2e-6898.55DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1BWW42.7e-6898.55DNA-directed RNA polymerase II subunit 4 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A5D3C0194.6e-6897.10DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A0A0K7V54.6e-6897.10RPOL4c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014650 PE=4... [more]
BLAST of Sed0026127 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G09920.11.8e-6993.48RNA polymerase II, Rpb4, core protein [more]
AT4G15950.18.1e-1737.93RNA polymerase II, Rpb4, core protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006590RNA polymerase Rpb4/RPC9, coreSMARTSM00657rpol4neu2coord: 16..138
e-value: 2.6E-59
score: 213.1
IPR005574RNA polymerase subunit RPB4/RPC9PFAMPF03874RNA_pol_Rpb4coord: 23..132
e-value: 8.4E-24
score: 84.3
IPR038324Rpb4/RPC9 superfamilyGENE3D1.20.1250.40coord: 2..138
e-value: 1.1E-51
score: 176.0
NoneNo IPR availablePANTHERPTHR21297:SF3DNA-DIRECTED RNA POLYMERASE II SUBUNIT 4-LIKEcoord: 1..138
NoneNo IPR availablePANTHERPTHR21297DNA-DIRECTED RNA POLYMERASE IIcoord: 1..138
IPR010997HRDC-like superfamilySUPERFAMILY47819HRDC-likecoord: 3..136

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0026127.1Sed0026127.1mRNA
Sed0026127.2Sed0026127.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031990 mRNA export from nucleus in response to heat stress
biological_process GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
biological_process GO:0045948 positive regulation of translational initiation
biological_process GO:0034402 recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
biological_process GO:0044237 cellular metabolic process
biological_process GO:0006352 DNA-templated transcription, initiation
cellular_component GO:0000932 P-body
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0030880 RNA polymerase complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0031369 translation initiation factor binding