Homology
BLAST of Sed0025718 vs. NCBI nr
Match:
XP_004133946.1 (ent-kaurenoic acid oxidase 1 isoform X1 [Cucumis sativus] >KAE8650281.1 hypothetical protein Csa_010193 [Cucumis sativus])
HSP 1 Score: 911.4 bits (2354), Expect = 3.4e-261
Identity = 446/491 (90.84%), Postives = 466/491 (94.91%), Query Frame = 0
Query: 1 MEGSWVVAAAATAV--AMIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGN 60
ME SW VAAA TA+ A V FAV+KWAAKSLNEWIYEAKLGDRRMALPPGDLGWP IGN
Sbjct: 2 MEVSWGVAAAVTALAGAAAVVFAVLKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGN 61
Query: 61 MLAFFRAFKSSNPETFIDSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQP 120
ML F RAFKS+NPETFIDSYVSRFGK GVYK+HLFGNPSVVVTTPETCRKVLTDD+AFQP
Sbjct: 62 MLGFLRAFKSNNPETFIDSYVSRFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQP 121
Query: 121 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSN 180
GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEAL+NYIPYIEENVL+SL+KWSN
Sbjct: 122 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSN 181
Query: 181 MGPIEFLTQLRKLTFKIIMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKA 240
MGPIEFLTQLRKLTF IIMYIFLS+ESE VME+LEKEYTRLNYGVRA+R+N+PGFAY+KA
Sbjct: 182 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKA 241
Query: 241 LKARKNLVAALQGIVTERRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMY 300
LKARKNLVAA QGIVTERR R+L A KRKDMMDALIDVEDE GRKLTDEEIIDILVMY
Sbjct: 242 LKARKNLVAAFQGIVTERRKRRLGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMY 301
Query: 301 LNAGHESSGHTMMWATILLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNK 360
LNAGHESSGHTMMWATILLN+HPEVLKKAK EQEEIV+RRPAGQKGLTLKECRDMEYL+K
Sbjct: 302 LNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSK 361
Query: 361 VVDETLRYVSFSLVVFRETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSR 420
VVDETLRYVSFSLVVFRE QMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSR
Sbjct: 362 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSR 421
Query: 421 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSR 480
WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEI IF+HYFLLNYKLEWLTPDCQILYLPHSR
Sbjct: 422 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSR 481
Query: 481 PKDNCIAKISK 490
PKDNC+AKISK
Sbjct: 482 PKDNCLAKISK 492
BLAST of Sed0025718 vs. NCBI nr
Match:
XP_038902869.1 (ent-kaurenoic acid oxidase 1-like [Benincasa hispida])
HSP 1 Score: 905.2 bits (2338), Expect = 2.4e-259
Identity = 442/489 (90.39%), Postives = 462/489 (94.48%), Query Frame = 0
Query: 1 MEGSWVVAAAATAVAMIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGNML 60
ME SW VAA A AVA FA VKWAAKSLNEWIYEAKLGDRRMALPPGDLGWP IGNML
Sbjct: 1 MEVSWAVAAVAAAVA---AFAAVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNML 60
Query: 61 AFFRAFKSSNPETFIDSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQPGW 120
AF RAFKS NPETFIDSYVSRFGK GVYKVHLFGNPSVVVTTPETCRKVLTDD+AFQPGW
Sbjct: 61 AFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGW 120
Query: 121 PRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSNMG 180
PR+A+ELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEAL+NYIPYIEE+VL+SL KWS+MG
Sbjct: 121 PRSAIELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEESVLKSLGKWSDMG 180
Query: 181 PIEFLTQLRKLTFKIIMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKALK 240
PIEFLTQLRKLTF IIMYIFLS+ESEPVMEALEKEYTRLNYGVRA+R+N PGFAY+KALK
Sbjct: 181 PIEFLTQLRKLTFTIIMYIFLSAESEPVMEALEKEYTRLNYGVRALRINFPGFAYHKALK 240
Query: 241 ARKNLVAALQGIVTERRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMYLN 300
ARKNLVAALQ IVT+RR R+LDK A KRKDMMDALIDVEDE GRKLTDEEIIDILVMYLN
Sbjct: 241 ARKNLVAALQRIVTDRRKRRLDKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLN 300
Query: 301 AGHESSGHTMMWATILLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNKVV 360
AGHESSGHTMMWATILLN+HPEVLKKAKAEQEEIV+RRP GQKGLTLKECR+MEYL+KVV
Sbjct: 301 AGHESSGHTMMWATILLNQHPEVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVV 360
Query: 361 DETLRYVSFSLVVFRETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSRWD 420
DETLRYVSFSLVVFRE QMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSRWD
Sbjct: 361 DETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWD 420
Query: 421 GFIPKAGEFLPFGAGSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSRPK 480
F+PKAGEFLPFGAGSRLCPGNDLAKLEI IF+HYFLLNYKLEWLTPDC ILYLPHSRPK
Sbjct: 421 NFVPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPK 480
Query: 481 DNCIAKISK 490
DNC+AKISK
Sbjct: 481 DNCLAKISK 486
BLAST of Sed0025718 vs. NCBI nr
Match:
TYK17483.1 (ent-kaurenoic acid oxidase 2-like [Cucumis melo var. makuwa])
HSP 1 Score: 903.7 bits (2334), Expect = 7.1e-259
Identity = 444/491 (90.43%), Postives = 461/491 (93.89%), Query Frame = 0
Query: 1 MEGSWVVAAAATAV--AMIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGN 60
ME SW AAA T V A V FAVVKWAAKS NEWIYEAKLG+RRMALPPGDLGWP IGN
Sbjct: 2 MEVSWGTAAAVTVVAGAAAVFFAVVKWAAKSFNEWIYEAKLGERRMALPPGDLGWPLIGN 61
Query: 61 MLAFFRAFKSSNPETFIDSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQP 120
ML F RAFKS NPETFIDSYVSRFGK GVYKVHLFGNPSVVVTTPETCRKVLTDD+AFQP
Sbjct: 62 MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP 121
Query: 121 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSN 180
GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEAL+NYIPYIEENVL+SL+KWS
Sbjct: 122 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWST 181
Query: 181 MGPIEFLTQLRKLTFKIIMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKA 240
MGPIEFLTQLRKLTF IIMYIFLS+ESE VME+LEKEYTRLNYGVRA+R+NLPGFAY+KA
Sbjct: 182 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGVRALRINLPGFAYHKA 241
Query: 241 LKARKNLVAALQGIVTERRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMY 300
LKARKNLVAALQGIVTERR R+L K A KRKDMMDALIDVEDE GRKLTDEEIIDILVMY
Sbjct: 242 LKARKNLVAALQGIVTERRKRRLGKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMY 301
Query: 301 LNAGHESSGHTMMWATILLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNK 360
LNAGHESSGHTMMWATILLN+HPEVLKKAK EQEEIV+RRP GQKGLTLKECR+MEYL+K
Sbjct: 302 LNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPPGQKGLTLKECREMEYLSK 361
Query: 361 VVDETLRYVSFSLVVFRETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSR 420
VVDETLRYVSFSLVVFRE QMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSR
Sbjct: 362 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSR 421
Query: 421 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSR 480
WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEI IF+HYFLLNYKLEWLTPDC ILYLPHSR
Sbjct: 422 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR 481
Query: 481 PKDNCIAKISK 490
PKDNC+AKISK
Sbjct: 482 PKDNCLAKISK 492
BLAST of Sed0025718 vs. NCBI nr
Match:
XP_008438234.1 (PREDICTED: ent-kaurenoic acid oxidase 2-like [Cucumis melo])
HSP 1 Score: 903.7 bits (2334), Expect = 7.1e-259
Identity = 444/491 (90.43%), Postives = 461/491 (93.89%), Query Frame = 0
Query: 1 MEGSWVVAAAATAV--AMIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGN 60
ME SW AAA T V A V FAVVKWAAKS NEWIYEAKLG+RRMALPPGDLGWP IGN
Sbjct: 2 MEVSWGTAAAVTVVAGAAAVFFAVVKWAAKSFNEWIYEAKLGERRMALPPGDLGWPLIGN 61
Query: 61 MLAFFRAFKSSNPETFIDSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQP 120
ML F RAFKS NPETFIDSYVSRFGK GVYKVHLFGNPSVVVTTPETCRKVLTDD+AFQP
Sbjct: 62 MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP 121
Query: 121 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSN 180
GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEAL+NYIPYIEENVL+SL+KWS
Sbjct: 122 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWST 181
Query: 181 MGPIEFLTQLRKLTFKIIMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKA 240
MGPIEFLTQLRKLTF IIMYIFLS+ESE VME+LEKEYTRLNYGVRA+R+NLPGFAY+KA
Sbjct: 182 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGVRALRINLPGFAYHKA 241
Query: 241 LKARKNLVAALQGIVTERRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMY 300
LKARKNLVAALQGIVTERR R+L K A KRKDMMDALIDVEDE GRKLTDEEIIDILVMY
Sbjct: 242 LKARKNLVAALQGIVTERRKRRLGKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMY 301
Query: 301 LNAGHESSGHTMMWATILLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNK 360
LNAGHESSGHTMMWATILLN+HPEVLKKAK EQEEIV+RRP GQKGLTLKECR+MEYL+K
Sbjct: 302 LNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPPGQKGLTLKECREMEYLSK 361
Query: 361 VVDETLRYVSFSLVVFRETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSR 420
VVDETLRYVSFSLVVFRE QMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSR
Sbjct: 362 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSR 421
Query: 421 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSR 480
WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEI IF+HYFLLNYKLEWLTPDC ILYLPHSR
Sbjct: 422 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR 481
Query: 481 PKDNCIAKISK 490
PKDNC+AKISK
Sbjct: 482 PKDNCLAKISK 492
BLAST of Sed0025718 vs. NCBI nr
Match:
XP_022924644.1 (ent-kaurenoic acid oxidase 2-like [Cucurbita moschata])
HSP 1 Score: 896.3 bits (2315), Expect = 1.1e-256
Identity = 433/491 (88.19%), Postives = 460/491 (93.69%), Query Frame = 0
Query: 1 MEGSWVVAAAATA-VAMIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGNM 60
ME +W VAA TA VA+ F+V+KWAAKSLNEWIYEAKLGDRR+ALPPGDLGWP IGNM
Sbjct: 1 MESTWAVAAVVTAVVAVATVFSVLKWAAKSLNEWIYEAKLGDRRLALPPGDLGWPLIGNM 60
Query: 61 LAFFRAFKSSNPETFIDSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQPG 120
L F RAFKS NPETFID YVSR+GKTGVYKVHLFGNPSVVVTTPETCRKVLTDD+AFQPG
Sbjct: 61 LGFLRAFKSKNPETFIDGYVSRYGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPG 120
Query: 121 WPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSNM 180
WPRAA+ELIG+KSFI+MP EEHKRLRRLTSAPVNGFEAL+NYIPYIE+NVLESLEKWS M
Sbjct: 121 WPRAAIELIGEKSFIQMPEEEHKRLRRLTSAPVNGFEALSNYIPYIEKNVLESLEKWSKM 180
Query: 181 GPIEFLTQLRKLTFKIIMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKAL 240
GPIEFLTQLRKLTF +IMYIFLSSESEPVME LEKEYTRLNYGVRAMR+NLPGFAY+KAL
Sbjct: 181 GPIEFLTQLRKLTFTVIMYIFLSSESEPVMEMLEKEYTRLNYGVRAMRINLPGFAYHKAL 240
Query: 241 KARKNLVAALQGIVTERRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMYL 300
KARKNLVAAL+GIVTERR +KLDK A KRKDMMD LID+EDE GRKL DEEIIDIL+MYL
Sbjct: 241 KARKNLVAALKGIVTERRRQKLDKWAPKRKDMMDQLIDIEDENGRKLDDEEIIDILIMYL 300
Query: 301 NAGHESSGHTMMWATILLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNKV 360
NAGHESSGHTMMWATILLN+HPEVLKKA+ EQE IV+ RPAGQ GLTLKECRDMEYL+KV
Sbjct: 301 NAGHESSGHTMMWATILLNQHPEVLKKAREEQEAIVRNRPAGQTGLTLKECRDMEYLSKV 360
Query: 361 VDETLRYVSFSLVVFRETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSRW 420
VDETLRYVSFSLVVFRE QMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSRW
Sbjct: 361 VDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRW 420
Query: 421 DGFIPKAGEFLPFGAGSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSRP 480
DGF+PKAGEFLPFGAGSRLCPGNDLAKLEI IFVHYFLLNY LEWLTPDC+ILYLPHSRP
Sbjct: 421 DGFVPKAGEFLPFGAGSRLCPGNDLAKLEICIFVHYFLLNYNLEWLTPDCEILYLPHSRP 480
Query: 481 KDNCIAKISKK 491
KDNC+AKI+KK
Sbjct: 481 KDNCMAKITKK 491
BLAST of Sed0025718 vs. ExPASy Swiss-Prot
Match:
O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)
HSP 1 Score: 698.0 bits (1800), Expect = 7.8e-200
Identity = 319/474 (67.30%), Postives = 397/474 (83.76%), Query Frame = 0
Query: 16 MIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGNMLAFFRAFKSSNPETFI 75
M++G + W + +N W+YE+ LG+ R LPPGDLGWPFIGNML+F RAFK+S+P++F
Sbjct: 15 MVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFT 74
Query: 76 DSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQPGWPRAAVELIGKKSFIE 135
+ + R+G G+YK H+FGNPS++VTT +TCR+VLTDDDAF+PGWP + +ELIG+KSF+
Sbjct: 75 RTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVG 134
Query: 136 MPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSNMGPIEFLTQLRKLTFKI 195
+ EEHKRLRRLT+APVNG EAL+ YIPYIEENV+ L+KW+ MG EFLT LRKLTF+I
Sbjct: 135 ISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTFRI 194
Query: 196 IMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKALKARKNLVAALQGIVTE 255
IMYIFLSSESE VM+ALE+EYT LNYGVRAM VN+PGFAY++ALKARK LVAA Q IVTE
Sbjct: 195 IMYIFLSSESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTE 254
Query: 256 RRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMYLNAGHESSGHTMMWATI 315
RR ++ +KDM+D L++V+DE G+ L DEEIID+L+MYLNAGHESSGHT+MWAT+
Sbjct: 255 RRNQRKQNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATV 314
Query: 316 LLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNKVVDETLRYVSFSLVVFR 375
L EHPEVL++AKAEQE I+K RP GQKGL+LKE R ME+L++VVDETLR ++FSL FR
Sbjct: 315 FLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFR 374
Query: 376 ETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSRWD-GFIPKAGEFLPFGA 435
E + DV +NGYLIPKGWKVL WFR +H DPEV+PDP+KFDP+RWD GF+PKAG FLPFGA
Sbjct: 375 EAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGA 434
Query: 436 GSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSRPKDNCIAKIS 489
GS LCPGNDLAKLEISIF+H+FLL Y+++ P+C ++YLPH+RP DNC+A+IS
Sbjct: 435 GSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARIS 488
BLAST of Sed0025718 vs. ExPASy Swiss-Prot
Match:
Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)
HSP 1 Score: 689.1 bits (1777), Expect = 3.6e-197
Identity = 320/474 (67.51%), Postives = 395/474 (83.33%), Query Frame = 0
Query: 16 MIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGNMLAFFRAFKSSNPETFI 75
+I+G V+KW K +N WIY +KLG+++ LPPGDLGWP IGNM +F RAFK+S+PE+FI
Sbjct: 14 IILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFI 73
Query: 76 DSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQPGWPRAAVELIGKKSFIE 135
SY++R+G+TG+YK H+FG P V+VTTPETCR+VLTDDDAF GWP++ ++LIG+KSF+
Sbjct: 74 QSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVG 133
Query: 136 MPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSNMGPIEFLTQLRKLTFKI 195
+ EEHKRLRRLTSAPVNG EAL+ YI +IEE V LEKWS MG IEFL+ LRKLTFK+
Sbjct: 134 ISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKV 193
Query: 196 IMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKALKARKNLVAALQGIVTE 255
IMYIFLSSESE VM++LE+EYT LNYGVRAM +NLPGFAY++ALKARK LVAA Q IVT
Sbjct: 194 IMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTN 253
Query: 256 RRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMYLNAGHESSGHTMMWATI 315
RR ++ + RKDM+D LIDV+DE GR L DEEIID+L+MYLNAGHESSGH MWATI
Sbjct: 254 RRNQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATI 313
Query: 316 LLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNKVVDETLRYVSFSLVVFR 375
L+ EHP +L+KAK EQE IVK+R GQK LTLKE R+M YL++V+DETLR ++FSL FR
Sbjct: 314 LMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFR 373
Query: 376 ETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSRWDGFIPKAGEFLPFGAG 435
E + DV ++GY+IPKGWKVL WFR++H DPE+YPDPKKFDPSRW+G+ PKAG FLPFG G
Sbjct: 374 EAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLG 433
Query: 436 SRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSRPKDNCIAKISK 490
S LCPGNDLAKLEISIF+H+FLL Y++E P C +++LPH+RPKDNC+A+I++
Sbjct: 434 SHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486
BLAST of Sed0025718 vs. ExPASy Swiss-Prot
Match:
Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)
HSP 1 Score: 604.0 bits (1556), Expect = 1.5e-171
Identity = 287/494 (58.10%), Postives = 370/494 (74.90%), Query Frame = 0
Query: 2 EGSWVVAAAATAVAMIVGFAVVKWAAKSLNEWIYEAKLG-DRRMALPPGDLGWPFIGNML 61
EG+W AA A +V A ++ + W + A LG RR LPPGD+GWP +G M
Sbjct: 3 EGAWWAVAAVVAALAVVALDA---AVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMW 62
Query: 62 AFFRAFKSSNPETFIDSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQPGW 121
AF RAFKS P++FIDS+ RFG+ G+Y+ +F +P+++ TTPE C++VL DDDAF GW
Sbjct: 63 AFLRAFKSGRPDSFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGW 122
Query: 122 PRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSNM- 181
P+A V LIG KSF+ M +EH+RLR+LT+AP+NGF+ALT+Y+ +I++ V+ +L WS
Sbjct: 123 PKATVALIGPKSFVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERG 182
Query: 182 --GPIEFLTQLRKLTFKIIMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYK 241
G EFLT+LR++TF+II+ IF+ E LE+ YT LNYG+RAM ++LPGFAY+K
Sbjct: 183 GDGHFEFLTELRRMTFRIIVQIFMGGADERTAAELERTYTELNYGMRAMAIDLPGFAYHK 242
Query: 242 ALKARKNLVAALQGIVTERRIR--KLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDIL 301
A++AR+ LVAALQ ++ ERR R K A DMMD LI VEDEGGR+L D+EIID+L
Sbjct: 243 AIRARRRLVAALQRVLDERRARGGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEIIDVL 302
Query: 302 VMYLNAGHESSGHTMMWATILLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEY 361
VMYLNAGHESSGH MWAT+ L E+PE+L KAKAEQE I++ P GQKGLTL++ R M Y
Sbjct: 303 VMYLNAGHESSGHITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAY 362
Query: 362 LNKVVDETLRYVSFSLVVFRETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFD 421
L++VVDETLR+V+ S V FR+ DV +NGYLIPKGWKV W+RS+H DP+VYPDPKKFD
Sbjct: 363 LSQVVDETLRFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFD 422
Query: 422 PSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLP 481
PSRW+G P+AG FLPFG G+RLCPGNDLAKLEIS+F+H+FLL YKL P+C++ YLP
Sbjct: 423 PSRWEGPPPRAGTFLPFGLGTRLCPGNDLAKLEISVFLHHFLLGYKLTRKNPNCRVRYLP 482
Query: 482 HSRPKDNCIAKISK 490
H RP DNC+AKI++
Sbjct: 483 HPRPVDNCLAKITR 493
BLAST of Sed0025718 vs. ExPASy Swiss-Prot
Match:
Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)
HSP 1 Score: 590.9 bits (1522), Expect = 1.3e-167
Identity = 279/487 (57.29%), Postives = 361/487 (74.13%), Query Frame = 0
Query: 5 WVVAAAATAVAMIVGFAVVKWAAKSLNEWIYEAKLG-DRRMALPPGDLGWPFIGNMLAFF 64
W AA + V VV AA+ + W EA LG RR LPPG++GWP +G M AF
Sbjct: 30 WYREAAEAVLVGAVALVVVDAAARRAHGWYREAALGAARRARLPPGEMGWPLVGGMWAFL 89
Query: 65 RAFKSSNPETFIDSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQPGWPRA 124
RAFKS P+ FI S+V RFG+TGVY+ +F +P+V+VTT E C++VL DDDAF GWP+A
Sbjct: 90 RAFKSGKPDAFIASFVRRFGRTGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKA 149
Query: 125 AVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSNM-GPI 184
V L+G +SF+ MP +EH+R+R+LT+AP+NGF+ALT Y+P+I+ V SL W++ G +
Sbjct: 150 TVALVGPRSFVAMPYDEHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSV 209
Query: 185 EFLTQLRKLTFKIIMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKALKAR 244
EFLT+LR++TFKII+ IFL + ALE+ YT LNYG+RAM +NLPGFAY AL+AR
Sbjct: 210 EFLTELRRMTFKIIVQIFLGGADQATTRALERSYTELNYGMRAMAINLPGFAYRGALRAR 269
Query: 245 KNLVAALQGIVTERRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMYLNAG 304
+ LVA LQG++ ERR + + DMMD LI+ +DE GR L D+EIID+LVMYLNAG
Sbjct: 270 RRLVAVLQGVLDERRAARAKGVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAG 329
Query: 305 HESSGHTMMWATILLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNKVVDE 364
HESSGH MWAT+ L E+P++ +AKAEQE I++ P+ Q+GLTL++ R MEYL++V+DE
Sbjct: 330 HESSGHITMWATVFLQENPDMFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDE 389
Query: 365 TLRYVSFSLVVFRETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSRWDGF 424
TLR V+ S V FR+ DV +NGYLIPKGWKV W+RS+H DP+VYPDP KFDPSRW+G
Sbjct: 390 TLRLVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGH 449
Query: 425 IPKAGEFLPFGAGSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSRPKDN 484
P+AG FL FG G+RLCPGNDLAKLEIS+F+H+FLL YKL P C++ YLPH RP DN
Sbjct: 450 SPRAGTFLAFGLGARLCPGNDLAKLEISVFLHHFLLGYKLARTNPRCRVRYLPHPRPVDN 509
Query: 485 CIAKISK 490
C+AKI++
Sbjct: 510 CLAKITR 516
BLAST of Sed0025718 vs. ExPASy Swiss-Prot
Match:
Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)
HSP 1 Score: 589.7 bits (1519), Expect = 3.0e-167
Identity = 277/501 (55.29%), Postives = 373/501 (74.45%), Query Frame = 0
Query: 1 MEGSWVVAAAAT------AVAMIVGFAVVKWAAKSLNEWIYEAKLG-DRRMALPPGDLGW 60
MEG + AA A A A++ G +V + ++W+ A LG +RR LPPG++GW
Sbjct: 3 MEGMGMAAAWAAGDLWVLAAAVVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGW 62
Query: 61 PFIGNMLAFFRAFKSSNPETFIDSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDD 120
P +G+M AF RAFKS NP+ FI S++ RFG+TGVY+ +F +P+++ TPE C++VL DD
Sbjct: 63 PMVGSMWAFLRAFKSGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDD 122
Query: 121 DAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESL 180
+ F GWP+A V LIG KSF+ M ++H+R+R+LT+AP+NGF+ALT Y+ +I++ V+ SL
Sbjct: 123 EGFVTGWPKATVTLIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASL 182
Query: 181 EKWSN--MGPIEFLTQLRKLTFKIIMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLP 240
+WS+ G +EFLT+LR++TFKII+ IF+S + MEALE+ YT LNYG+RAM +NLP
Sbjct: 183 RRWSSPESGQVEFLTELRRMTFKIIVQIFMSGADDATMEALERSYTDLNYGMRAMAINLP 242
Query: 241 GFAYYKALKARKNLVAALQGIVTERRIRKLDKSAVKRK---DMMDALIDVEDEGGRKLTD 300
GFAYY+AL+AR+ LV+ LQG++ RR KR DMMD LI+ EDE GR+L D
Sbjct: 243 GFAYYRALRARRKLVSVLQGVLDGRRAAA--AKGFKRSGAMDMMDRLIEAEDERGRRLAD 302
Query: 301 EEIIDILVMYLNAGHESSGHTMMWATILLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLK 360
+EI+D+L+MYLNAGHESSGH MWAT+ L E+P++ +AKAEQEEI++ PA Q GLTL+
Sbjct: 303 DEIVDVLIMYLNAGHESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLR 362
Query: 361 ECRDMEYLNKVVDETLRYVSFSLVVFRETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVY 420
+ + M +L++VVDETLR V+ S V FR+ D+ +NGYLIPKGWKV W+RS+H D +VY
Sbjct: 363 DFKKMHFLSQVVDETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVY 422
Query: 421 PDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPD 480
PDPK F+PSRW+G PKAG FLPFG G+RLCPGNDLAKLEIS+F+H+FLL YKL+ P
Sbjct: 423 PDPKMFNPSRWEGPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPK 482
Query: 481 CQILYLPHSRPKDNCIAKISK 490
C++ YLPH RP DNC+A I+K
Sbjct: 483 CRVRYLPHPRPVDNCLATITK 501
BLAST of Sed0025718 vs. ExPASy TrEMBL
Match:
A0A5D3CZZ0 (Ent-kaurenoic acid oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003480 PE=3 SV=1)
HSP 1 Score: 903.7 bits (2334), Expect = 3.4e-259
Identity = 444/491 (90.43%), Postives = 461/491 (93.89%), Query Frame = 0
Query: 1 MEGSWVVAAAATAV--AMIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGN 60
ME SW AAA T V A V FAVVKWAAKS NEWIYEAKLG+RRMALPPGDLGWP IGN
Sbjct: 2 MEVSWGTAAAVTVVAGAAAVFFAVVKWAAKSFNEWIYEAKLGERRMALPPGDLGWPLIGN 61
Query: 61 MLAFFRAFKSSNPETFIDSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQP 120
ML F RAFKS NPETFIDSYVSRFGK GVYKVHLFGNPSVVVTTPETCRKVLTDD+AFQP
Sbjct: 62 MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP 121
Query: 121 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSN 180
GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEAL+NYIPYIEENVL+SL+KWS
Sbjct: 122 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWST 181
Query: 181 MGPIEFLTQLRKLTFKIIMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKA 240
MGPIEFLTQLRKLTF IIMYIFLS+ESE VME+LEKEYTRLNYGVRA+R+NLPGFAY+KA
Sbjct: 182 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGVRALRINLPGFAYHKA 241
Query: 241 LKARKNLVAALQGIVTERRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMY 300
LKARKNLVAALQGIVTERR R+L K A KRKDMMDALIDVEDE GRKLTDEEIIDILVMY
Sbjct: 242 LKARKNLVAALQGIVTERRKRRLGKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMY 301
Query: 301 LNAGHESSGHTMMWATILLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNK 360
LNAGHESSGHTMMWATILLN+HPEVLKKAK EQEEIV+RRP GQKGLTLKECR+MEYL+K
Sbjct: 302 LNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPPGQKGLTLKECREMEYLSK 361
Query: 361 VVDETLRYVSFSLVVFRETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSR 420
VVDETLRYVSFSLVVFRE QMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSR
Sbjct: 362 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSR 421
Query: 421 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSR 480
WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEI IF+HYFLLNYKLEWLTPDC ILYLPHSR
Sbjct: 422 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR 481
Query: 481 PKDNCIAKISK 490
PKDNC+AKISK
Sbjct: 482 PKDNCLAKISK 492
BLAST of Sed0025718 vs. ExPASy TrEMBL
Match:
A0A1S3AWI4 (ent-kaurenoic acid oxidase 2-like OS=Cucumis melo OX=3656 GN=LOC103483403 PE=3 SV=1)
HSP 1 Score: 903.7 bits (2334), Expect = 3.4e-259
Identity = 444/491 (90.43%), Postives = 461/491 (93.89%), Query Frame = 0
Query: 1 MEGSWVVAAAATAV--AMIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGN 60
ME SW AAA T V A V FAVVKWAAKS NEWIYEAKLG+RRMALPPGDLGWP IGN
Sbjct: 2 MEVSWGTAAAVTVVAGAAAVFFAVVKWAAKSFNEWIYEAKLGERRMALPPGDLGWPLIGN 61
Query: 61 MLAFFRAFKSSNPETFIDSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQP 120
ML F RAFKS NPETFIDSYVSRFGK GVYKVHLFGNPSVVVTTPETCRKVLTDD+AFQP
Sbjct: 62 MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP 121
Query: 121 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSN 180
GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEAL+NYIPYIEENVL+SL+KWS
Sbjct: 122 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWST 181
Query: 181 MGPIEFLTQLRKLTFKIIMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKA 240
MGPIEFLTQLRKLTF IIMYIFLS+ESE VME+LEKEYTRLNYGVRA+R+NLPGFAY+KA
Sbjct: 182 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGVRALRINLPGFAYHKA 241
Query: 241 LKARKNLVAALQGIVTERRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMY 300
LKARKNLVAALQGIVTERR R+L K A KRKDMMDALIDVEDE GRKLTDEEIIDILVMY
Sbjct: 242 LKARKNLVAALQGIVTERRKRRLGKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMY 301
Query: 301 LNAGHESSGHTMMWATILLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNK 360
LNAGHESSGHTMMWATILLN+HPEVLKKAK EQEEIV+RRP GQKGLTLKECR+MEYL+K
Sbjct: 302 LNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPPGQKGLTLKECREMEYLSK 361
Query: 361 VVDETLRYVSFSLVVFRETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSR 420
VVDETLRYVSFSLVVFRE QMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSR
Sbjct: 362 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSR 421
Query: 421 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSR 480
WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEI IF+HYFLLNYKLEWLTPDC ILYLPHSR
Sbjct: 422 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR 481
Query: 481 PKDNCIAKISK 490
PKDNC+AKISK
Sbjct: 482 PKDNCLAKISK 492
BLAST of Sed0025718 vs. ExPASy TrEMBL
Match:
A0A6J1E9S4 (ent-kaurenoic acid oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432076 PE=3 SV=1)
HSP 1 Score: 896.3 bits (2315), Expect = 5.5e-257
Identity = 433/491 (88.19%), Postives = 460/491 (93.69%), Query Frame = 0
Query: 1 MEGSWVVAAAATA-VAMIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGNM 60
ME +W VAA TA VA+ F+V+KWAAKSLNEWIYEAKLGDRR+ALPPGDLGWP IGNM
Sbjct: 1 MESTWAVAAVVTAVVAVATVFSVLKWAAKSLNEWIYEAKLGDRRLALPPGDLGWPLIGNM 60
Query: 61 LAFFRAFKSSNPETFIDSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQPG 120
L F RAFKS NPETFID YVSR+GKTGVYKVHLFGNPSVVVTTPETCRKVLTDD+AFQPG
Sbjct: 61 LGFLRAFKSKNPETFIDGYVSRYGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPG 120
Query: 121 WPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSNM 180
WPRAA+ELIG+KSFI+MP EEHKRLRRLTSAPVNGFEAL+NYIPYIE+NVLESLEKWS M
Sbjct: 121 WPRAAIELIGEKSFIQMPEEEHKRLRRLTSAPVNGFEALSNYIPYIEKNVLESLEKWSKM 180
Query: 181 GPIEFLTQLRKLTFKIIMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKAL 240
GPIEFLTQLRKLTF +IMYIFLSSESEPVME LEKEYTRLNYGVRAMR+NLPGFAY+KAL
Sbjct: 181 GPIEFLTQLRKLTFTVIMYIFLSSESEPVMEMLEKEYTRLNYGVRAMRINLPGFAYHKAL 240
Query: 241 KARKNLVAALQGIVTERRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMYL 300
KARKNLVAAL+GIVTERR +KLDK A KRKDMMD LID+EDE GRKL DEEIIDIL+MYL
Sbjct: 241 KARKNLVAALKGIVTERRRQKLDKWAPKRKDMMDQLIDIEDENGRKLDDEEIIDILIMYL 300
Query: 301 NAGHESSGHTMMWATILLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNKV 360
NAGHESSGHTMMWATILLN+HPEVLKKA+ EQE IV+ RPAGQ GLTLKECRDMEYL+KV
Sbjct: 301 NAGHESSGHTMMWATILLNQHPEVLKKAREEQEAIVRNRPAGQTGLTLKECRDMEYLSKV 360
Query: 361 VDETLRYVSFSLVVFRETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSRW 420
VDETLRYVSFSLVVFRE QMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSRW
Sbjct: 361 VDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRW 420
Query: 421 DGFIPKAGEFLPFGAGSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSRP 480
DGF+PKAGEFLPFGAGSRLCPGNDLAKLEI IFVHYFLLNY LEWLTPDC+ILYLPHSRP
Sbjct: 421 DGFVPKAGEFLPFGAGSRLCPGNDLAKLEICIFVHYFLLNYNLEWLTPDCEILYLPHSRP 480
Query: 481 KDNCIAKISKK 491
KDNC+AKI+KK
Sbjct: 481 KDNCMAKITKK 491
BLAST of Sed0025718 vs. ExPASy TrEMBL
Match:
A0A6J1IVT9 (ent-kaurenoic acid oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479695 PE=3 SV=1)
HSP 1 Score: 892.5 bits (2305), Expect = 7.9e-256
Identity = 432/491 (87.98%), Postives = 459/491 (93.48%), Query Frame = 0
Query: 1 MEGSWVVAAAATA-VAMIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGNM 60
ME +W VAA TA VA+ F+V+KWAAKSLNEWIYEAKLGDRR+ALPPGDLGWP IGNM
Sbjct: 1 MESTWAVAAVVTAVVAVATVFSVLKWAAKSLNEWIYEAKLGDRRLALPPGDLGWPLIGNM 60
Query: 61 LAFFRAFKSSNPETFIDSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQPG 120
L F RAFKS NPETFID YVSR+GKTGVYKVHLFGNPSVVVTTPETCRKVLTDD+AFQPG
Sbjct: 61 LGFLRAFKSKNPETFIDGYVSRYGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPG 120
Query: 121 WPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSNM 180
WPRAAVELIG+KSFI+MP EEHKRLRRLTSAPVNGFEAL+NYIPYIE+NVLESLEKWS M
Sbjct: 121 WPRAAVELIGEKSFIQMPQEEHKRLRRLTSAPVNGFEALSNYIPYIEKNVLESLEKWSKM 180
Query: 181 GPIEFLTQLRKLTFKIIMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKAL 240
GPIEFLTQLRKLTF +IMYIFLSSESEPVME LEKEYTRLNYGVRAMR+NLPGFAY+KAL
Sbjct: 181 GPIEFLTQLRKLTFTVIMYIFLSSESEPVMEMLEKEYTRLNYGVRAMRINLPGFAYHKAL 240
Query: 241 KARKNLVAALQGIVTERRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMYL 300
KARKNLVAAL+GIVTERR +KLDK A KRKDMMD LID+ DE GRKL DEEIIDIL+MYL
Sbjct: 241 KARKNLVAALKGIVTERRRQKLDKWAPKRKDMMDQLIDIVDENGRKLDDEEIIDILIMYL 300
Query: 301 NAGHESSGHTMMWATILLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNKV 360
NAGHESSGHTMMWATILLN+HPEVLKKA+ EQE IV+ RPAGQ GLTLKECRDMEYL+KV
Sbjct: 301 NAGHESSGHTMMWATILLNQHPEVLKKAREEQEAIVRNRPAGQTGLTLKECRDMEYLSKV 360
Query: 361 VDETLRYVSFSLVVFRETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSRW 420
VDETLRYVSFSLVVFRE QMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKF+PSRW
Sbjct: 361 VDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFEPSRW 420
Query: 421 DGFIPKAGEFLPFGAGSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSRP 480
DGF+PKAGEFLPFGAGSRLCPGNDLAKLEI IFVHYFLLNY LEWLTPDC+ILYLPHSRP
Sbjct: 421 DGFVPKAGEFLPFGAGSRLCPGNDLAKLEICIFVHYFLLNYNLEWLTPDCEILYLPHSRP 480
Query: 481 KDNCIAKISKK 491
KDNC+AKI+KK
Sbjct: 481 KDNCMAKITKK 491
BLAST of Sed0025718 vs. ExPASy TrEMBL
Match:
Q9FQY4 (Ent-kaurenoic acid oxidase OS=Cucurbita maxima OX=3661 GN=CYP88A2 PE=2 SV=1)
HSP 1 Score: 890.2 bits (2299), Expect = 3.9e-255
Identity = 431/491 (87.78%), Postives = 458/491 (93.28%), Query Frame = 0
Query: 1 MEGSWVVAAAATA-VAMIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGNM 60
ME +W VAA TA VA+ F+V+KWAAKSLNEWIYEAKLGDRR+ALPPGDLGWP IGNM
Sbjct: 1 MESTWAVAAVVTAVVAVATVFSVLKWAAKSLNEWIYEAKLGDRRLALPPGDLGWPLIGNM 60
Query: 61 LAFFRAFKSSNPETFIDSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQPG 120
L F RAFKS NPETFID YVSR+GKTGVYKVHLFGNPSVVVTTPETCRKVLTDD+AFQPG
Sbjct: 61 LGFLRAFKSKNPETFIDGYVSRYGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPG 120
Query: 121 WPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSNM 180
WPRAAVELIG+KSFI+MP EEHKRLRRLTSAPVNGFEAL+NYIPYIE+NVLESLEKWS M
Sbjct: 121 WPRAAVELIGEKSFIQMPQEEHKRLRRLTSAPVNGFEALSNYIPYIEKNVLESLEKWSKM 180
Query: 181 GPIEFLTQLRKLTFKIIMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKAL 240
GPIEFLTQLRKLTF +IMYIFLSSESEPVME LEKEYTRLNYGVR MR+NLPGFAY+KAL
Sbjct: 181 GPIEFLTQLRKLTFTVIMYIFLSSESEPVMEMLEKEYTRLNYGVRDMRINLPGFAYHKAL 240
Query: 241 KARKNLVAALQGIVTERRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMYL 300
KARKNLVAAL+GIVTERR +KLDK A KRKDMMD LID+ DE GRKL DEEIIDIL+MYL
Sbjct: 241 KARKNLVAALKGIVTERRRQKLDKWAPKRKDMMDQLIDIVDENGRKLDDEEIIDILIMYL 300
Query: 301 NAGHESSGHTMMWATILLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNKV 360
NAGHESSGHTMMWATILLN+HPEVLKKA+ EQE IV+ RPAGQ GLTLKECRDMEYL+KV
Sbjct: 301 NAGHESSGHTMMWATILLNQHPEVLKKAREEQEAIVRNRPAGQTGLTLKECRDMEYLSKV 360
Query: 361 VDETLRYVSFSLVVFRETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSRW 420
VDETLRYVSFSLVVFRE QMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKF+PSRW
Sbjct: 361 VDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFEPSRW 420
Query: 421 DGFIPKAGEFLPFGAGSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSRP 480
DGF+PKAGEFLPFGAGSRLCPGNDLAKLEI IFVHYFLLNY LEWLTPDC+ILYLPHSRP
Sbjct: 421 DGFVPKAGEFLPFGAGSRLCPGNDLAKLEICIFVHYFLLNYNLEWLTPDCEILYLPHSRP 480
Query: 481 KDNCIAKISKK 491
KDNC+AKI+KK
Sbjct: 481 KDNCMAKITKK 491
BLAST of Sed0025718 vs. TAIR 10
Match:
AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )
HSP 1 Score: 698.0 bits (1800), Expect = 5.5e-201
Identity = 319/474 (67.30%), Postives = 397/474 (83.76%), Query Frame = 0
Query: 16 MIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGNMLAFFRAFKSSNPETFI 75
M++G + W + +N W+YE+ LG+ R LPPGDLGWPFIGNML+F RAFK+S+P++F
Sbjct: 15 MVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFT 74
Query: 76 DSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQPGWPRAAVELIGKKSFIE 135
+ + R+G G+YK H+FGNPS++VTT +TCR+VLTDDDAF+PGWP + +ELIG+KSF+
Sbjct: 75 RTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVG 134
Query: 136 MPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSNMGPIEFLTQLRKLTFKI 195
+ EEHKRLRRLT+APVNG EAL+ YIPYIEENV+ L+KW+ MG EFLT LRKLTF+I
Sbjct: 135 ISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTFRI 194
Query: 196 IMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKALKARKNLVAALQGIVTE 255
IMYIFLSSESE VM+ALE+EYT LNYGVRAM VN+PGFAY++ALKARK LVAA Q IVTE
Sbjct: 195 IMYIFLSSESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTE 254
Query: 256 RRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMYLNAGHESSGHTMMWATI 315
RR ++ +KDM+D L++V+DE G+ L DEEIID+L+MYLNAGHESSGHT+MWAT+
Sbjct: 255 RRNQRKQNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATV 314
Query: 316 LLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNKVVDETLRYVSFSLVVFR 375
L EHPEVL++AKAEQE I+K RP GQKGL+LKE R ME+L++VVDETLR ++FSL FR
Sbjct: 315 FLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFR 374
Query: 376 ETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSRWD-GFIPKAGEFLPFGA 435
E + DV +NGYLIPKGWKVL WFR +H DPEV+PDP+KFDP+RWD GF+PKAG FLPFGA
Sbjct: 375 EAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGA 434
Query: 436 GSRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSRPKDNCIAKIS 489
GS LCPGNDLAKLEISIF+H+FLL Y+++ P+C ++YLPH+RP DNC+A+IS
Sbjct: 435 GSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARIS 488
BLAST of Sed0025718 vs. TAIR 10
Match:
AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )
HSP 1 Score: 689.1 bits (1777), Expect = 2.6e-198
Identity = 320/474 (67.51%), Postives = 395/474 (83.33%), Query Frame = 0
Query: 16 MIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGNMLAFFRAFKSSNPETFI 75
+I+G V+KW K +N WIY +KLG+++ LPPGDLGWP IGNM +F RAFK+S+PE+FI
Sbjct: 14 IILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFI 73
Query: 76 DSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQPGWPRAAVELIGKKSFIE 135
SY++R+G+TG+YK H+FG P V+VTTPETCR+VLTDDDAF GWP++ ++LIG+KSF+
Sbjct: 74 QSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVG 133
Query: 136 MPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSNMGPIEFLTQLRKLTFKI 195
+ EEHKRLRRLTSAPVNG EAL+ YI +IEE V LEKWS MG IEFL+ LRKLTFK+
Sbjct: 134 ISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKV 193
Query: 196 IMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKALKARKNLVAALQGIVTE 255
IMYIFLSSESE VM++LE+EYT LNYGVRAM +NLPGFAY++ALKARK LVAA Q IVT
Sbjct: 194 IMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTN 253
Query: 256 RRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMYLNAGHESSGHTMMWATI 315
RR ++ + RKDM+D LIDV+DE GR L DEEIID+L+MYLNAGHESSGH MWATI
Sbjct: 254 RRNQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATI 313
Query: 316 LLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNKVVDETLRYVSFSLVVFR 375
L+ EHP +L+KAK EQE IVK+R GQK LTLKE R+M YL++V+DETLR ++FSL FR
Sbjct: 314 LMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFR 373
Query: 376 ETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSRWDGFIPKAGEFLPFGAG 435
E + DV ++GY+IPKGWKVL WFR++H DPE+YPDPKKFDPSRW+G+ PKAG FLPFG G
Sbjct: 374 EAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLG 433
Query: 436 SRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSRPKDNCIAKISK 490
S LCPGNDLAKLEISIF+H+FLL Y++E P C +++LPH+RPKDNC+A+I++
Sbjct: 434 SHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486
BLAST of Sed0025718 vs. TAIR 10
Match:
AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )
HSP 1 Score: 689.1 bits (1777), Expect = 2.6e-198
Identity = 320/474 (67.51%), Postives = 395/474 (83.33%), Query Frame = 0
Query: 16 MIVGFAVVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPFIGNMLAFFRAFKSSNPETFI 75
+I+G V+KW K +N WIY +KLG+++ LPPGDLGWP IGNM +F RAFK+S+PE+FI
Sbjct: 14 IILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFI 73
Query: 76 DSYVSRFGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDDAFQPGWPRAAVELIGKKSFIE 135
SY++R+G+TG+YK H+FG P V+VTTPETCR+VLTDDDAF GWP++ ++LIG+KSF+
Sbjct: 74 QSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVG 133
Query: 136 MPVEEHKRLRRLTSAPVNGFEALTNYIPYIEENVLESLEKWSNMGPIEFLTQLRKLTFKI 195
+ EEHKRLRRLTSAPVNG EAL+ YI +IEE V LEKWS MG IEFL+ LRKLTFK+
Sbjct: 134 ISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKV 193
Query: 196 IMYIFLSSESEPVMEALEKEYTRLNYGVRAMRVNLPGFAYYKALKARKNLVAALQGIVTE 255
IMYIFLSSESE VM++LE+EYT LNYGVRAM +NLPGFAY++ALKARK LVAA Q IVT
Sbjct: 194 IMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTN 253
Query: 256 RRIRKLDKSAVKRKDMMDALIDVEDEGGRKLTDEEIIDILVMYLNAGHESSGHTMMWATI 315
RR ++ + RKDM+D LIDV+DE GR L DEEIID+L+MYLNAGHESSGH MWATI
Sbjct: 254 RRNQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATI 313
Query: 316 LLNEHPEVLKKAKAEQEEIVKRRPAGQKGLTLKECRDMEYLNKVVDETLRYVSFSLVVFR 375
L+ EHP +L+KAK EQE IVK+R GQK LTLKE R+M YL++V+DETLR ++FSL FR
Sbjct: 314 LMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFR 373
Query: 376 ETQMDVNLNGYLIPKGWKVLAWFRSIHYDPEVYPDPKKFDPSRWDGFIPKAGEFLPFGAG 435
E + DV ++GY+IPKGWKVL WFR++H DPE+YPDPKKFDPSRW+G+ PKAG FLPFG G
Sbjct: 374 EAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLG 433
Query: 436 SRLCPGNDLAKLEISIFVHYFLLNYKLEWLTPDCQILYLPHSRPKDNCIAKISK 490
S LCPGNDLAKLEISIF+H+FLL Y++E P C +++LPH+RPKDNC+A+I++
Sbjct: 434 SHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486
BLAST of Sed0025718 vs. TAIR 10
Match:
AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )
HSP 1 Score: 276.2 bits (705), Expect = 5.2e-74
Identity = 160/450 (35.56%), Postives = 253/450 (56.22%), Query Frame = 0
Query: 34 IYEAKLGDRRMALPPGDLGWPFIGNMLAFFRAFKSSNPETFIDSYVSRFGKTGVYKVHLF 93
I K + R LPPG +GWP++G L + S NP F S R+G+ ++K +
Sbjct: 22 IVSKKKKNSRGKLPPGSMGWPYLGETLQLY----SQNPNVFFTSKQKRYGE--IFKTRIL 81
Query: 94 GNPSVVVTTPETCRKVL-TDDDAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPV 153
G P V++ +PE R VL T F+P +PR+ +LIG + + H +R+L +
Sbjct: 82 GYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSF 141
Query: 154 NGFEALTNYIPYIEENVLESLEKWSNMGPIEFLTQLRKLTFKI-IMYIFLSSESEPVMEA 213
E + IP IE L SL+ W+NM + +++K F + I+ IF ES E
Sbjct: 142 YP-ETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAIFGHLESS-YKEI 201
Query: 214 LEKEYTRLNYGVRAMRVNLPGFAYYKALKARKNLVAALQGIVTERRIRKLDKSAVKRKDM 273
L+ Y ++ G + ++LPG +Y+KAL ARK L + I+ ERR +K A+ + D
Sbjct: 202 LKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERR----EKRAL-QTDF 261
Query: 274 MDALIDVEDEGGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNEHPEVLKKAKAEQ 333
+ L++ ++E GR LT E+I D ++ L A +++ + W L++ ++L+ KAEQ
Sbjct: 262 LGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAEQ 321
Query: 334 EEIVKRRPAGQKGLTLKECRDMEYLNKVVDETLRYVSFSLVVFRETQMDVNLNGYLIPKG 393
+ I + +K LT ++ R+M +KV+ E+LR S FRE +DV GYLIPKG
Sbjct: 322 KAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPKG 381
Query: 394 WKVLAWFRSIHYDPEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEISI 453
WKV+ FR+IH++P+ + +P+ FDPSR++ PK F+PFG+G CPGN+LAKL+I I
Sbjct: 382 WKVMPLFRNIHHNPKYFSNPEVFDPSRFE-VNPKPNTFMPFGSGVHACPGNELAKLQILI 441
Query: 454 FVHYFLLNYKLEWLTPDCQILYLPHSRPKD 482
F+H+ + N++ E + I Y P P++
Sbjct: 442 FLHHLVSNFRWEVKGGEKGIQYSPFPIPQN 457
BLAST of Sed0025718 vs. TAIR 10
Match:
AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )
HSP 1 Score: 263.8 bits (673), Expect = 2.7e-70
Identity = 154/454 (33.92%), Postives = 242/454 (53.30%), Query Frame = 0
Query: 34 IYEAKLGDRRMALPPGDLGWPFIGNMLAFFRAFKSSNPETFIDSYVSRFGKTGVYKVHLF 93
I + + G ++ LPPG +GWP++G + S +P F S R+G V+K H+
Sbjct: 24 ISQRRFGSSKLPLPPGTMGWPYVGETFQLY----SQDPNVFFQSKQKRYG--SVFKTHVL 83
Query: 94 GNPSVVVTTPETCRKVL-TDDDAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPV 153
G P V++++PE + VL T F+P +P + ++GK++ + H +LR+L +
Sbjct: 84 GCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLV---L 143
Query: 154 NGF--EALTNYIPYIEENVLESLEKWSNMGPIEFLTQLRKLTFKIIMYIFLSSESEPVME 213
F E++ N +P IE +SL W I +++ TF + + + E
Sbjct: 144 RAFMPESIRNMVPDIESIAQDSLRSWEGT-MINTYQEMKTYTFNVALLSIFGKDEVLYRE 203
Query: 214 ALEKEYTRLNYGVRAMRVNLPGFAYYKALKARKNLVAALQGIVTERRIRKLDKSAVKRKD 273
L++ Y L G +M VNLPG ++K++KARK L L I++ERR ++ D
Sbjct: 204 DLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERR-----QNGSSHND 263
Query: 274 MMDALIDVEDEGGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNEHPEVLKKAKAE 333
++ + + ++E LTDE+I D ++ + A +++ M W L E+P VL+ E
Sbjct: 264 LLGSFMGDKEE----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEE 323
Query: 334 QEEIVKRRPAGQKGLTLKECRDMEYLNKVVDETLRYVSFSLVVFRETQMDVNLNGYLIPK 393
Q I K + G+ LT + + M ++V+ ETLR S FRE DV GYLIPK
Sbjct: 324 QMAIRKDKEEGE-SLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPK 383
Query: 394 GWKVLAWFRSIHYDPEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEIS 453
GWKVL FR+IH+ +++ +P KFDPSR++ PK F+PFG G+ CPGN+LAKLE+S
Sbjct: 384 GWKVLPLFRNIHHSADIFSNPGKFDPSRFE-VAPKPNTFMPFGNGTHSCPGNELAKLEMS 443
Query: 454 IFVHYFLLNYKLEWLTPDCQILYLPHSR-PKDNC 484
I +H+ Y+ L + HSR PK +C
Sbjct: 444 IMIHHLTTKYRFGQLLERATGFSMGHSRFPKTDC 456
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_004133946.1 | 3.4e-261 | 90.84 | ent-kaurenoic acid oxidase 1 isoform X1 [Cucumis sativus] >KAE8650281.1 hypothet... | [more] |
XP_038902869.1 | 2.4e-259 | 90.39 | ent-kaurenoic acid oxidase 1-like [Benincasa hispida] | [more] |
TYK17483.1 | 7.1e-259 | 90.43 | ent-kaurenoic acid oxidase 2-like [Cucumis melo var. makuwa] | [more] |
XP_008438234.1 | 7.1e-259 | 90.43 | PREDICTED: ent-kaurenoic acid oxidase 2-like [Cucumis melo] | [more] |
XP_022924644.1 | 1.1e-256 | 88.19 | ent-kaurenoic acid oxidase 2-like [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
O23051 | 7.8e-200 | 67.30 | Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1 | [more] |
Q9C5Y2 | 3.6e-197 | 67.51 | Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2 | [more] |
Q9AXH9 | 1.5e-171 | 58.10 | Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1 | [more] |
Q43246 | 1.3e-167 | 57.29 | Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1 | [more] |
Q5VRM7 | 3.0e-167 | 55.29 | Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A5D3CZZ0 | 3.4e-259 | 90.43 | Ent-kaurenoic acid oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... | [more] |
A0A1S3AWI4 | 3.4e-259 | 90.43 | ent-kaurenoic acid oxidase 2-like OS=Cucumis melo OX=3656 GN=LOC103483403 PE=3 S... | [more] |
A0A6J1E9S4 | 5.5e-257 | 88.19 | ent-kaurenoic acid oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432076 ... | [more] |
A0A6J1IVT9 | 7.9e-256 | 87.98 | ent-kaurenoic acid oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479695 PE... | [more] |
Q9FQY4 | 3.9e-255 | 87.78 | Ent-kaurenoic acid oxidase OS=Cucurbita maxima OX=3661 GN=CYP88A2 PE=2 SV=1 | [more] |