Sed0025609 (gene) Chayote v1

BLAST of Sed0025609 vs. NCBI nr
Match: XP_023537955.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1051.2 bits (2717), Expect = 3.9e-303
Identity = 527/623 (84.59%), Postives = 577/623 (92.62%), Query Frame = 0

Query: 1   MQGTEEREIKMVDPKNEGKGGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLET 60
           MQG+EE+EI +VDP NEGKGGFRATMFIFALLTFE+MGFVANMVSLVQYFL+V+HFDL+T
Sbjct: 1   MQGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLLVLHFDLQT 60

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKP 120
           AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLE+LALV++ VQAYSHDLLPK 
Sbjct: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKA 120

Query: 121 ECPKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYML 180
           +CPKDCVRGRIAFVFYTSLYLLA+GSGGVRGALPALGADQF+QKDPKEAKAL TFFN +L
Sbjct: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180

Query: 181 LSIVIGAAVGVTVIVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILR 240
           LS+VIGAAVGVT+IVWVAVNKAWYWGFFISA ATA+GF+VFA+GKPFYRLH PGQSP+LR
Sbjct: 181 LSVVIGAAVGVTLIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240

Query: 241 IIEVIVVAFKNRGLSLPDRASELYELSDKCCMDTIDSKIAHTNQLRFFDKAAILAKDSEP 300
           II+VIVVA KNRGL+LPD  SELYELS+K  MD+IDSKIAHTNQLR  DKA+IL KD EP
Sbjct: 241 IIQVIVVAIKNRGLALPDTPSELYELSNKYYMDSIDSKIAHTNQLRCLDKASILPKDVEP 300

Query: 301 QAWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNFGHIQFP 360
           Q WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNT IMDK+ GH+QFP
Sbjct: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360

Query: 361 APSIPVIPLVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLV 420
           APSIPVIPLVFMA LIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA+SM VAGLV
Sbjct: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420

Query: 421 EVKRRNQATKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480
           EVKRR++ATKHP+E +SLFWLSFQY IFG+ADMFTLVGLLEFFYKEAPVGMRSLSTSFTW
Sbjct: 421 EVKRRHRATKHPNEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480

Query: 481 LSLSFGYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNF 540
           LSLSFGYYLSSIFVNV+NKVT+K++PSKKGWVEG IPEDLNYNNL+LFYWFLA+LS+LNF
Sbjct: 481 LSLSFGYYLSSIFVNVINKVTRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540

Query: 541 FHYLYWASWYKYKAEEPIVQLNGGGGG----EEGVPILQKDEERSVGDVTNHQNKGKEDV 600
           FHYLYWASWYKYK EE IV+L+ GGGG    EEGVPIL ++EERS GD+  H ++GKED 
Sbjct: 541 FHYLYWASWYKYKTEETIVKLDNGGGGEREREEGVPIL-REEERSGGDIKCHHSEGKEDA 600

Query: 601 PILRENEERSGVDIQDHHSEGKE 620
           PI  E EERS +D++   SE KE
Sbjct: 601 PIFHEEEERSEIDVKGRQSEEKE 622

BLAST of Sed0025609 vs. NCBI nr
Match: KAG6586059.1 (Protein NRT1/ PTR FAMILY 4.5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1050.4 bits (2715), Expect = 6.6e-303
Identity = 527/623 (84.59%), Postives = 575/623 (92.30%), Query Frame = 0

Query: 1   MQGTEEREIKMVDPKNEGKGGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLET 60
           MQG+EE+EI +VDP NEGKGGFRATMFIFALLTFE+MGFVANMVSLVQYFLMV+HFDL+T
Sbjct: 1   MQGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKP 120
           AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLE+LALV++ VQAYSHDLLPK 
Sbjct: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKA 120

Query: 121 ECPKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYML 180
           +CPKDCVRGRIAFVFYTSLYLLA+GSGGVRGALPALGADQF+QKDPKEAKAL TFFN +L
Sbjct: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180

Query: 181 LSIVIGAAVGVTVIVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILR 240
           LS+VIGAAVGVT+IVWVAVNKAWYWGFFISA ATA+GF+VFA+GKPFYRLH PGQSP+LR
Sbjct: 181 LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240

Query: 241 IIEVIVVAFKNRGLSLPDRASELYELSDKCCMDTIDSKIAHTNQLRFFDKAAILAKDSEP 300
           II+VIVVA KNRGL+LPD  +ELYELSDK  MD+IDSKI HTNQLR  DKA+IL KD EP
Sbjct: 241 IIQVIVVAVKNRGLALPDTPNELYELSDKYYMDSIDSKITHTNQLRCLDKASILPKDVEP 300

Query: 301 QAWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNFGHIQFP 360
           Q WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNT IMDK+ GH+QFP
Sbjct: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360

Query: 361 APSIPVIPLVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLV 420
           APSIPVIPLVFMA LIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA+SM VAGLV
Sbjct: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420

Query: 421 EVKRRNQATKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480
           EVKRR+QATKHPDE +SLFWLSFQY IFG+ADMFTLVGLLEFFYKEAPVGMRSLSTSFTW
Sbjct: 421 EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480

Query: 481 LSLSFGYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNF 540
           LSLSFGYYLSSIFVNV+NK+T+K++PSKKGWVEG IPEDLNYNNL+LFYWFLA+LS+LNF
Sbjct: 481 LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540

Query: 541 FHYLYWASWYKYKAEEPIVQLNGGGGG----EEGVPILQKDEERSVGDVTNHQNKGKEDV 600
           FHYLYWASWYKYK EE IV+L+ GGGG    EEGVPIL ++EERS GD+  H ++ KED 
Sbjct: 541 FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPIL-REEERSGGDIKCHHSEEKEDA 600

Query: 601 PILRENEERSGVDIQDHHSEGKE 620
           PI  E EERS +D++   SE KE
Sbjct: 601 PIFHEEEERSEIDLKGRQSEEKE 622

BLAST of Sed0025609 vs. NCBI nr
Match: XP_022938323.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita moschata])

HSP 1 Score: 1049.3 bits (2712), Expect = 1.5e-302
Identity = 527/623 (84.59%), Postives = 575/623 (92.30%), Query Frame = 0

Query: 1   MQGTEEREIKMVDPKNEGKGGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLET 60
           MQG+EE+EI +V P NEGKGGFRATMFIFALLTFE+MGFVANMVSLVQYFLMV+HFDL+T
Sbjct: 1   MQGSEEKEINVVGPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKP 120
           AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLE+LALV++ VQAYSHDLLPK 
Sbjct: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKA 120

Query: 121 ECPKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYML 180
           +CPKDCVRGRIAFVFYTSLYLLA+GSGGVRGALPALGADQF+QKDPKEAKAL TFFN +L
Sbjct: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180

Query: 181 LSIVIGAAVGVTVIVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILR 240
           LS+VIGAAVGVT+IVWVAVNKAWYWGFFISA ATA+GF+VFA+GKPFYRLH PGQSP+LR
Sbjct: 181 LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240

Query: 241 IIEVIVVAFKNRGLSLPDRASELYELSDKCCMDTIDSKIAHTNQLRFFDKAAILAKDSEP 300
           II+VIVVA KNRGL+LPD  +ELYELSDK  MD+IDSKIAHTNQLR  DKA+IL KD EP
Sbjct: 241 IIQVIVVAVKNRGLALPDTPNELYELSDKYYMDSIDSKIAHTNQLRCLDKASILPKDVEP 300

Query: 301 QAWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNFGHIQFP 360
           Q WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNT IMDK+ GH+QFP
Sbjct: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360

Query: 361 APSIPVIPLVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLV 420
           APSIPVIPLVFMA LIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA+SM VAGLV
Sbjct: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420

Query: 421 EVKRRNQATKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480
           EVKRR+QATKHPDE +SLFWLSFQY IFG+ADMFTLVGLLEFFYKEAPVGMRSLSTSFTW
Sbjct: 421 EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480

Query: 481 LSLSFGYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNF 540
           LSLSFGYYLSSIFVNV+NK+T+K++PSKKGWVEG IPEDLNYNNL+LFYWFLA+LS+LNF
Sbjct: 481 LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540

Query: 541 FHYLYWASWYKYKAEEPIVQLNGGGGG----EEGVPILQKDEERSVGDVTNHQNKGKEDV 600
           FHYLYWASWYKYK EE IV+L+ GGGG    EEGVPIL ++EERS GD+  H ++ KED 
Sbjct: 541 FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPIL-REEERSGGDIKCHHSEEKEDA 600

Query: 601 PILRENEERSGVDIQDHHSEGKE 620
           PI  E EERS +D++   SE KE
Sbjct: 601 PIFHEEEERSEIDLKGRQSEEKE 622

BLAST of Sed0025609 vs. NCBI nr
Match: XP_022969591.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita maxima])

HSP 1 Score: 1035.4 bits (2676), Expect = 2.2e-298
Identity = 522/623 (83.79%), Postives = 568/623 (91.17%), Query Frame = 0

Query: 1   MQGTEEREIKMVDPKNEGKGGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLET 60
           MQG+EE+EI +VDP NEGKGGFRATMFIFALLTFE+MGFVANMVSLVQYFLMV+HFDL+T
Sbjct: 1   MQGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKP 120
           AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLE+LALV++ VQAYSHDLLPK 
Sbjct: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKA 120

Query: 121 ECPKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYML 180
           +CPKDCV GRIAFVFYTSL LLA+GSGGVRGALPALGADQF+QKDPKEAKAL TFFN +L
Sbjct: 121 DCPKDCVHGRIAFVFYTSLCLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180

Query: 181 LSIVIGAAVGVTVIVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILR 240
           LS+VIGAAVGVT+IVWVAVNKAWYWGFFISA ATA+GF+VFA+GKPFYRLH PGQSP+LR
Sbjct: 181 LSVVIGAAVGVTLIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240

Query: 241 IIEVIVVAFKNRGLSLPDRASELYELSDKCCMDTIDSKIAHTNQLRFFDKAAILAKDSEP 300
           II+VIVVA KNRGL+LP+  SELYELSDK  MD+I+SKIA T QLRF DKA+IL KD EP
Sbjct: 241 IIQVIVVAIKNRGLALPNTPSELYELSDKYYMDSIESKIARTYQLRFLDKASILPKDVEP 300

Query: 301 QAWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNFGHIQFP 360
           Q WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNT IMDK+ GH+QFP
Sbjct: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTKIMDKSLGHLQFP 360

Query: 361 APSIPVIPLVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLV 420
           APSIPVIPLVFMA LIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA+SM VAGLV
Sbjct: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420

Query: 421 EVKRRNQATKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480
           E+KRR+QATKHPDE +SLFWLSFQY IFG+ADMFTLVGLLEFFYKEAPVGMRSLSTSFTW
Sbjct: 421 EMKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480

Query: 481 LSLSFGYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNF 540
           LSLSFGYYLSSIFVNV+NKVT+K++PSKKGWVEG IPEDLNYNNL+LFYWFLA+LS+LNF
Sbjct: 481 LSLSFGYYLSSIFVNVINKVTRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540

Query: 541 FHYLYWASWYKYKAEEPIVQLNGGGGG----EEGVPILQKDEERSVGDVTNHQNKGKEDV 600
           FHYLYWASWYKYK EEPIV+LN GGGG    EE VPIL   EERS G +  H ++ KE  
Sbjct: 541 FHYLYWASWYKYKTEEPIVKLNDGGGGEREREESVPILH--EERSGGAIKCHHSEEKEHA 600

Query: 601 PILRENEERSGVDIQDHHSEGKE 620
           PI  E EER  +D++  HSE  E
Sbjct: 601 PIFHEEEERCEIDVKGRHSEENE 621

BLAST of Sed0025609 vs. NCBI nr
Match: XP_022156380.1 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 4.5-like [Momordica charantia])

HSP 1 Score: 996.9 bits (2576), Expect = 8.6e-287
Identity = 516/635 (81.26%), Postives = 559/635 (88.03%), Query Frame = 0

Query: 3   GTEEREIKMVDPKNEGKGGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLETAA 62
           G+E REIK VDP N+GKGGFRAT+FIFALL+FENMGFVANMVSLVQYFL+VMHFDL+TAA
Sbjct: 71  GSEAREIKDVDPSNKGKGGFRATIFIFALLSFENMGFVANMVSLVQYFLLVMHFDLQTAA 130

Query: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKPEC 122
           NTLTNFLGSAFLLSLLGGFLSDTYINRLTT LIFGVLEILALVMI VQAYS DLLPKP+C
Sbjct: 131 NTLTNFLGSAFLLSLLGGFLSDTYINRLTTSLIFGVLEILALVMITVQAYSQDLLPKPDC 190

Query: 123 PKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYMLLS 182
            KDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGA QFDQKDP+EAKAL TFFN MLLS
Sbjct: 191 LKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGAXQFDQKDPREAKALPTFFNCMLLS 250

Query: 183 IVIGAAVGVTVIVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILRII 242
           +V+GAAVGVTVIVWVAVNKAW+WGF ISA ATA+GFVVFA+GKPFYRLH PG+SP+ RII
Sbjct: 251 VVVGAAVGVTVIVWVAVNKAWFWGFLISAVATAVGFVVFAVGKPFYRLHVPGESPVSRII 310

Query: 243 EVIVVAFKNRGLSLPDRASELYELSDKCCMDTIDSKIAHTNQLRFFDKAAILAKDSEPQA 302
           +VIVVA +NR L LPD  SELYE+SDK  +D+IDSKIAHTNQLRF DKAAIL  DSE QA
Sbjct: 311 QVIVVAIRNRRLPLPDSPSELYEISDKYYLDSIDSKIAHTNQLRFLDKAAILPNDSEAQA 370

Query: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNFGHIQFPAP 362
           WKVCSVTQVEEVKIITRM+PIFISTIIMNTCLAQLQTFSVEQGNT IMD++ GH+QFPAP
Sbjct: 371 WKVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTTIMDRSLGHLQFPAP 430

Query: 363 SIPVIPLVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLVEV 422
           SIPVIPLVFMA LIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISM VAGLVEV
Sbjct: 431 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 490

Query: 423 KRRNQATKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482
           KRRNQAT HP++ +SLFWLSFQY IFG+ADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS
Sbjct: 491 KRRNQATNHPNQPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 550

Query: 483 LSFGYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNFFH 542
           LSFGYYLSSIFVNVVNKVT+ I PSKKGWVEGQIPEDLN+NNL+LFYWFLA+LSILNFFH
Sbjct: 551 LSFGYYLSSIFVNVVNKVTRNIAPSKKGWVEGQIPEDLNHNNLNLFYWFLAILSILNFFH 610

Query: 543 YLYWASWYKYKAEEPIVQL-----------------NGGGGGEEGVPILQKDEERSVGDV 602
           YLYWASWYKYK EEPI +L                 NGGGGGEEG  I+Q  + R+ G+ 
Sbjct: 611 YLYWASWYKYKTEEPIAELDGGGGEDAPIVQDNRASNGGGGGEEGASIIQ--DNRAEGE- 670

Query: 603 TNHQNKGKEDVPILRENEERSGVDIQ-DHHSEGKE 620
                 G+E  PIL++ E   G D++ D +SE KE
Sbjct: 671 ------GEEGAPILQKEE---GSDVKADRYSEEKE 693

BLAST of Sed0025609 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 3.2e-111
Identity = 230/547 (42.05%), Postives = 326/547 (59.60%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLETAANTLTNFLGSAFLLSLLG 79
           GG  A  F+ A+   EN+ F+AN  +LV Y    MH  L  +++ +T F+ +AFLL+LLG
Sbjct: 29  GGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLG 88

Query: 80  GFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKPECPK------DCVRGRIAF 139
           GFL+D + +     LI   +E L L+++ +QA    L+P P C        + V G  A 
Sbjct: 89  GFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMP-PPCKSSAALRCEVVGGSKAA 148

Query: 140 VFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYMLLSIVIGAAVGVTV 199
             +  LYL+++G GG++G+LP+ GA+QFD+  PK  K   TFFNY +  +  GA V VT 
Sbjct: 149 FLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTF 208

Query: 200 IVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILRIIEVIVVAFKNRG 259
           +VW+  NK W WGF +S  +  L  +VF +G  FY+   P  SP+  I +V++ A     
Sbjct: 209 VVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAA---SI 268

Query: 260 LSLPDRASELYELSDKCCMDTIDSKIAH--TNQLRFFDKAAILAKDSEPQAWKVCSVTQV 319
           +S   + S  +  S +   +  +   +   TN L   +KA    +      W  C+V QV
Sbjct: 269 VSCSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKA---IEGKTHHIWLECTVQQV 328

Query: 320 EEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNFGHIQFPAPSIPVIPLVF 379
           E+VKI+ +MLPIF  TI++N CLAQL T+SV Q  T  M++   +   P+ S+PV P+VF
Sbjct: 329 EDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFPVVF 388

Query: 380 MALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLVEVKRRNQATK- 439
           M +L P Y+ + +PFARK+T    GIT LQR+GVGLVLS ++M VA LVE+KR+  A + 
Sbjct: 389 MLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREA 448

Query: 440 -----HPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLSF 499
                     I+  W++ QY   G AD+FTL GLLEFF+ EAP  MRSL+TS +W SL+ 
Sbjct: 449 GLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLAL 508

Query: 500 GYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNFFHYLY 553
           GYYLSS+ V +VN+VTK  +  +  W    + E LN N LDLFYW + VLS++NF HYL+
Sbjct: 509 GYYLSSVMVPIVNRVTK--SAGQSPW----LGEKLNRNRLDLFYWLMCVLSVVNFLHYLF 560

BLAST of Sed0025609 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 4.7e-110
Identity = 230/546 (42.12%), Postives = 332/546 (60.81%), Query Frame = 0

Query: 14  PKNEGK-GGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLETAANTLTNFLGSA 73
           P N  K GG RA +F+  L  FE MG  A   +L+ Y +  MHF L  AAN +TNF+G+ 
Sbjct: 36  PSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTI 95

Query: 74  FLLSLLGGFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKPEC----PKDC-- 133
           F+ +LLGG+LSD ++    T +IFG +E+   ++++VQA+   L P P+C     + C  
Sbjct: 96  FIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKP-PKCNPLIDQTCEE 155

Query: 134 VRGRIAFVFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYMLLSIVIG 193
            +G  A +F+ +LYL+A+GSG V+  + A GADQF Q  PK++K L ++FN    +  +G
Sbjct: 156 AKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMG 215

Query: 194 AAVGVTVIVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILRIIEVIV 253
             + +T++VWV  +     GF +SA+A  +G +    G  ++R   P +S    I  VIV
Sbjct: 216 ELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIV 275

Query: 254 VAFKNRGLSLPDRASELYELSDKCCMDTIDSKIAHTNQLRFFDKAAILAKD--SEPQAWK 313
            A   R L+ P     L+            S + HT + RF DKA I  +D  ++   W+
Sbjct: 276 AAILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWR 335

Query: 314 VCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTL-IMDKNFGHIQFPAPS 373
           +C+VTQVE+VK +  ++PIF STI+ NT LAQLQTFSV+QG+++     N  HI  P  S
Sbjct: 336 LCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHI--PPAS 395

Query: 374 IPVIPLVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLVEVK 433
           +  IP + +  L+PLY+   VPFARK+T H SGI  L R+G+GL LS  SM  A ++E K
Sbjct: 396 LQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKK 455

Query: 434 RRNQATKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSL 493
           RR+ +       +S+FW++ Q+ IFG+++MFT VGL+EFFYK++  GM S   + T+ S 
Sbjct: 456 RRDSSVL-DGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSY 515

Query: 494 SFGYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNFFHY 550
           SFG+Y SS+ V+VVNK+T     S KGW+      DLN + LDLFYW LAVLS+LNF  Y
Sbjct: 516 SFGFYFSSVLVSVVNKITSTSVDS-KGWLG---ENDLNKDRLDLFYWLLAVLSLLNFLSY 573

BLAST of Sed0025609 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 6.8e-109
Identity = 227/566 (40.11%), Postives = 328/566 (57.95%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLETAANTLTNFLGSAFLLSLLG 79
           GG  A  F+  +   EN+ ++AN  +LV Y    MH     +AN +TNF+G+AFLL+LLG
Sbjct: 27  GGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLG 86

Query: 80  GFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKPECPK---DCVRGRIAFVFY 139
           GFLSD + +     LI   +E L L+++ +QA +  L+P P C     + V G  A + +
Sbjct: 87  GFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMP-PSCDSPTCEEVSGSKAAMLF 146

Query: 140 TSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYMLLSIVIGAAVGVTVIVW 199
             LYL+A+G GG++G+L + GA+QFD+  PK  K   TFFNY +  +  GA V VT +VW
Sbjct: 147 VGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVW 206

Query: 200 VAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILRIIEVIVVAFK---NRG 259
           +  NK W WGF +S  A  +  ++F  G  FYR   P  SP+  I++V++ A     + G
Sbjct: 207 LEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSG 266

Query: 260 LSLPDRASELYELSDKCCM------------------DTIDSKIAHTNQLRFFDKAAILA 319
            S    AS     S+ C                    + +  +   TN L+  + AA   
Sbjct: 267 SSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAA--- 326

Query: 320 KDSEP-QAWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNF 379
            D +P      C+V QVE+VKI+ +MLPIF  TI++N CLAQL TFSV+Q  +  M+   
Sbjct: 327 -DEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAAS--MNTKI 386

Query: 380 GHIQFPAPSIPVIPLVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISM 439
           G ++ P  S+P+ P+VF+ +L P+Y+ + +PFARK T   +G+T LQR+GVGLVLS ++M
Sbjct: 387 GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAM 446

Query: 440 GVAGLVEVKRRNQA------TKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAP 499
            VA LVE+KR+  A             ++  W++ QY   G AD+FTL GLLE+F+ EAP
Sbjct: 447 AVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAP 506

Query: 500 VGMRSLSTSFTWLSLSFGYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLF 555
             MRSL+TS +W SL+ GYYLSS+ V++VN +T   +     W+ G   + +N   LD F
Sbjct: 507 SSMRSLATSLSWASLAMGYYLSSVIVSIVNSITG--SSGNTPWLRG---KSINRYKLDYF 566

BLAST of Sed0025609 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 7.7e-105
Identity = 226/543 (41.62%), Postives = 329/543 (60.59%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLETAANTLTNFLGSAFLLSLLG 79
           GG RA +F+     FE M   A   +L+ Y    MHF L  +AN +TNF+G+ FLLSLLG
Sbjct: 40  GGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLG 99

Query: 80  GFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKPECPK-----DCV--RGRIA 139
           GFLSD+Y+    T L+FGV+EI   ++++VQA+  +L P PEC        CV   G  A
Sbjct: 100 GFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRP-PECNMKSTTIHCVEANGYKA 159

Query: 140 FVFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYMLLSIVIGAAVGVT 199
              YT+L L+A+GSG ++  + + GA+QF +KD    + L +FFN    +  +G  + +T
Sbjct: 160 ATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALT 219

Query: 200 VIVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILRIIEVIVVAFKNR 259
           ++VWV  +     GF +SA+  A G +    G  FYR   P  S    I +V V A   R
Sbjct: 220 LLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKR 279

Query: 260 GLSLPDRASELYELSDKCCMDTIDSK-IAHTNQLRFFDKAAILAKDSEPQA-WKVCSVTQ 319
               P   + +++ S     D +  K + H+N+ RF DKA I  +    ++ W++C++ Q
Sbjct: 280 KQICPSNPNMVHQPS----TDLVRVKPLLHSNKFRFLDKACIKTQGKAMESPWRLCTIEQ 339

Query: 320 VEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTL--IMDKNFGHIQFPAPSIPVIP 379
           V +VKI+  ++PIF  TII NT LAQLQTFSV+QG+++   + K F   Q P  S+  IP
Sbjct: 340 VHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKTF---QIPPASLQAIP 399

Query: 380 LVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLVEVKRRNQA 439
            + +   +PLYE  FVP ARK+T + SGI+ LQR+G GL L+  SM  A LVE KRR ++
Sbjct: 400 YIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRR-ES 459

Query: 440 TKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLSFGYY 499
               +  +S+FW++ Q+ IFG+++MFT VGL+EFFYK++   M+S  T+ T+ S SFG+Y
Sbjct: 460 FLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFY 519

Query: 500 LSSIFVNVVNKVTKKI-TPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNFFHYLYWA 551
           LSS+ V+ VN+VT    + +K+GW+      DLN + LD FYW LA LS +NFF+YL+W+
Sbjct: 520 LSSVLVSTVNRVTSSNGSGTKEGWLG---DNDLNKDRLDHFYWLLASLSFINFFNYLFWS 567

BLAST of Sed0025609 vs. ExPASy Swiss-Prot
Match: Q9LSE8 (Protein NRT1/ PTR FAMILY 4.2 OS=Arabidopsis thaliana OX=3702 GN=NPF4.2 PE=2 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.5e-100
Identity = 210/536 (39.18%), Postives = 324/536 (60.45%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLETAANTLTNFLGSAFLLSLLG 79
           GG RA   +  ++  EN+ F+AN  + V+YF+  MH+   TAAN +TNF+G++FLL+L G
Sbjct: 23  GGIRAASIVCVVVMMENIVFIANGFNFVKYFMGSMHYTPATAANMVTNFMGTSFLLTLFG 82

Query: 80  GFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKPECPKDCVRGRIAFVFYTSL 139
           GF++D+++   TT ++F  +E++ L+++  QA++  LLP+ +     ++  I F   T L
Sbjct: 83  GFIADSFVTHFTTFIVFCCIELMGLILLTFQAHNPKLLPEKDKTPSTLQSAILF---TGL 142

Query: 140 YLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYMLLSIVIGAAVGVTVIVWVAV 199
           Y +A+G+GG++ +LP+ G DQ D+++P   + +  FF+++  SI  G  + VTV++W+  
Sbjct: 143 YAMAIGTGGLKASLPSHGGDQIDRRNP---RLISRFFDWLYFSICSGCLLAVTVVLWIEE 202

Query: 200 NKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILRIIEVIVVAFKNRGLSLPDR 259
            K W W F IS    A    +F +G PFYR   P  SP+ +I  VI+ A +NR  S    
Sbjct: 203 KKGWIWSFNISVGILATALCIFTVGLPFYRFKRPNGSPLKKIAIVIISAARNRNKS---- 262

Query: 260 ASELYELSDKCCMDTID-SKIAHTNQLRFFDKAAILAKDSEPQAWKVCSVTQVEEVKIIT 319
                +L ++     I   K    N+L++ DKA +    SE         T+VEE +   
Sbjct: 263 -----DLDEEMMRGLISIYKNNSHNKLKWIDKATLNKNISE---------TEVEETRTFL 322

Query: 320 RMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNFGH-IQFPAPSIPVIPLVFMALLIP 379
            +LPIF STI+M+ C+AQL TFS +QG  ++M+K   H  + P PS+  IPL+FM L IP
Sbjct: 323 GLLPIFGSTIVMSCCVAQLSTFSAQQG--MLMNKKLFHSFEIPVPSLTAIPLIFMLLSIP 382

Query: 380 LYEFVFVPFARKIT---HHPSGITQLQRVGVGLVLSAISMGVAGLVEVKRRNQATKHPDE 439
           LYEF    F +KI+   ++ S    L+R+G+GL LS++SM V+ +VE KR+++   H + 
Sbjct: 383 LYEF----FGKKISSGNNNRSSSFNLKRIGLGLALSSVSMAVSAIVEAKRKHEVV-HNNF 442

Query: 440 QISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLSFGYYLSSIFV 499
           +IS+ WL FQY +  V+DM TL G+LEFFY+EAP  M+S+ST+  W S + G++LS+  V
Sbjct: 443 RISVLWLVFQYLMLSVSDMLTLGGMLEFFYREAPSNMKSISTALGWCSTALGFFLSTTLV 502

Query: 500 NVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNFFHYLYWASWY 551
            V N VT ++      W+ G   EDLN   L+LFY  L VL+ LN  +Y++WA  Y
Sbjct: 503 EVTNAVTGRLGHQ---WLGG---EDLNKTRLELFYVLLCVLNTLNLLNYIFWAKRY 521

BLAST of Sed0025609 vs. ExPASy TrEMBL
Match: A0A6J1FIK0 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111444459 PE=3 SV=1)

HSP 1 Score: 1049.3 bits (2712), Expect = 7.1e-303
Identity = 527/623 (84.59%), Postives = 575/623 (92.30%), Query Frame = 0

Query: 1   MQGTEEREIKMVDPKNEGKGGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLET 60
           MQG+EE+EI +V P NEGKGGFRATMFIFALLTFE+MGFVANMVSLVQYFLMV+HFDL+T
Sbjct: 1   MQGSEEKEINVVGPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKP 120
           AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLE+LALV++ VQAYSHDLLPK 
Sbjct: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKA 120

Query: 121 ECPKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYML 180
           +CPKDCVRGRIAFVFYTSLYLLA+GSGGVRGALPALGADQF+QKDPKEAKAL TFFN +L
Sbjct: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180

Query: 181 LSIVIGAAVGVTVIVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILR 240
           LS+VIGAAVGVT+IVWVAVNKAWYWGFFISA ATA+GF+VFA+GKPFYRLH PGQSP+LR
Sbjct: 181 LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240

Query: 241 IIEVIVVAFKNRGLSLPDRASELYELSDKCCMDTIDSKIAHTNQLRFFDKAAILAKDSEP 300
           II+VIVVA KNRGL+LPD  +ELYELSDK  MD+IDSKIAHTNQLR  DKA+IL KD EP
Sbjct: 241 IIQVIVVAVKNRGLALPDTPNELYELSDKYYMDSIDSKIAHTNQLRCLDKASILPKDVEP 300

Query: 301 QAWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNFGHIQFP 360
           Q WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNT IMDK+ GH+QFP
Sbjct: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360

Query: 361 APSIPVIPLVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLV 420
           APSIPVIPLVFMA LIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA+SM VAGLV
Sbjct: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420

Query: 421 EVKRRNQATKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480
           EVKRR+QATKHPDE +SLFWLSFQY IFG+ADMFTLVGLLEFFYKEAPVGMRSLSTSFTW
Sbjct: 421 EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480

Query: 481 LSLSFGYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNF 540
           LSLSFGYYLSSIFVNV+NK+T+K++PSKKGWVEG IPEDLNYNNL+LFYWFLA+LS+LNF
Sbjct: 481 LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540

Query: 541 FHYLYWASWYKYKAEEPIVQLNGGGGG----EEGVPILQKDEERSVGDVTNHQNKGKEDV 600
           FHYLYWASWYKYK EE IV+L+ GGGG    EEGVPIL ++EERS GD+  H ++ KED 
Sbjct: 541 FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPIL-REEERSGGDIKCHHSEEKEDA 600

Query: 601 PILRENEERSGVDIQDHHSEGKE 620
           PI  E EERS +D++   SE KE
Sbjct: 601 PIFHEEEERSEIDLKGRQSEEKE 622

BLAST of Sed0025609 vs. ExPASy TrEMBL
Match: A0A6J1HY86 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111468570 PE=3 SV=1)

HSP 1 Score: 1035.4 bits (2676), Expect = 1.1e-298
Identity = 522/623 (83.79%), Postives = 568/623 (91.17%), Query Frame = 0

Query: 1   MQGTEEREIKMVDPKNEGKGGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLET 60
           MQG+EE+EI +VDP NEGKGGFRATMFIFALLTFE+MGFVANMVSLVQYFLMV+HFDL+T
Sbjct: 1   MQGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKP 120
           AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLE+LALV++ VQAYSHDLLPK 
Sbjct: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKA 120

Query: 121 ECPKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYML 180
           +CPKDCV GRIAFVFYTSL LLA+GSGGVRGALPALGADQF+QKDPKEAKAL TFFN +L
Sbjct: 121 DCPKDCVHGRIAFVFYTSLCLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180

Query: 181 LSIVIGAAVGVTVIVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILR 240
           LS+VIGAAVGVT+IVWVAVNKAWYWGFFISA ATA+GF+VFA+GKPFYRLH PGQSP+LR
Sbjct: 181 LSVVIGAAVGVTLIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240

Query: 241 IIEVIVVAFKNRGLSLPDRASELYELSDKCCMDTIDSKIAHTNQLRFFDKAAILAKDSEP 300
           II+VIVVA KNRGL+LP+  SELYELSDK  MD+I+SKIA T QLRF DKA+IL KD EP
Sbjct: 241 IIQVIVVAIKNRGLALPNTPSELYELSDKYYMDSIESKIARTYQLRFLDKASILPKDVEP 300

Query: 301 QAWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNFGHIQFP 360
           Q WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNT IMDK+ GH+QFP
Sbjct: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTKIMDKSLGHLQFP 360

Query: 361 APSIPVIPLVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLV 420
           APSIPVIPLVFMA LIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA+SM VAGLV
Sbjct: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420

Query: 421 EVKRRNQATKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480
           E+KRR+QATKHPDE +SLFWLSFQY IFG+ADMFTLVGLLEFFYKEAPVGMRSLSTSFTW
Sbjct: 421 EMKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480

Query: 481 LSLSFGYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNF 540
           LSLSFGYYLSSIFVNV+NKVT+K++PSKKGWVEG IPEDLNYNNL+LFYWFLA+LS+LNF
Sbjct: 481 LSLSFGYYLSSIFVNVINKVTRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540

Query: 541 FHYLYWASWYKYKAEEPIVQLNGGGGG----EEGVPILQKDEERSVGDVTNHQNKGKEDV 600
           FHYLYWASWYKYK EEPIV+LN GGGG    EE VPIL   EERS G +  H ++ KE  
Sbjct: 541 FHYLYWASWYKYKTEEPIVKLNDGGGGEREREESVPILH--EERSGGAIKCHHSEEKEHA 600

Query: 601 PILRENEERSGVDIQDHHSEGKE 620
           PI  E EER  +D++  HSE  E
Sbjct: 601 PIFHEEEERCEIDVKGRHSEENE 621

BLAST of Sed0025609 vs. ExPASy TrEMBL
Match: A0A6J1DQ47 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 4.5-like OS=Momordica charantia OX=3673 GN=LOC111023287 PE=3 SV=1)

HSP 1 Score: 996.9 bits (2576), Expect = 4.2e-287
Identity = 516/635 (81.26%), Postives = 559/635 (88.03%), Query Frame = 0

Query: 3   GTEEREIKMVDPKNEGKGGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLETAA 62
           G+E REIK VDP N+GKGGFRAT+FIFALL+FENMGFVANMVSLVQYFL+VMHFDL+TAA
Sbjct: 71  GSEAREIKDVDPSNKGKGGFRATIFIFALLSFENMGFVANMVSLVQYFLLVMHFDLQTAA 130

Query: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKPEC 122
           NTLTNFLGSAFLLSLLGGFLSDTYINRLTT LIFGVLEILALVMI VQAYS DLLPKP+C
Sbjct: 131 NTLTNFLGSAFLLSLLGGFLSDTYINRLTTSLIFGVLEILALVMITVQAYSQDLLPKPDC 190

Query: 123 PKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYMLLS 182
            KDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGA QFDQKDP+EAKAL TFFN MLLS
Sbjct: 191 LKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGAXQFDQKDPREAKALPTFFNCMLLS 250

Query: 183 IVIGAAVGVTVIVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILRII 242
           +V+GAAVGVTVIVWVAVNKAW+WGF ISA ATA+GFVVFA+GKPFYRLH PG+SP+ RII
Sbjct: 251 VVVGAAVGVTVIVWVAVNKAWFWGFLISAVATAVGFVVFAVGKPFYRLHVPGESPVSRII 310

Query: 243 EVIVVAFKNRGLSLPDRASELYELSDKCCMDTIDSKIAHTNQLRFFDKAAILAKDSEPQA 302
           +VIVVA +NR L LPD  SELYE+SDK  +D+IDSKIAHTNQLRF DKAAIL  DSE QA
Sbjct: 311 QVIVVAIRNRRLPLPDSPSELYEISDKYYLDSIDSKIAHTNQLRFLDKAAILPNDSEAQA 370

Query: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNFGHIQFPAP 362
           WKVCSVTQVEEVKIITRM+PIFISTIIMNTCLAQLQTFSVEQGNT IMD++ GH+QFPAP
Sbjct: 371 WKVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTTIMDRSLGHLQFPAP 430

Query: 363 SIPVIPLVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLVEV 422
           SIPVIPLVFMA LIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISM VAGLVEV
Sbjct: 431 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 490

Query: 423 KRRNQATKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482
           KRRNQAT HP++ +SLFWLSFQY IFG+ADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS
Sbjct: 491 KRRNQATNHPNQPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 550

Query: 483 LSFGYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNFFH 542
           LSFGYYLSSIFVNVVNKVT+ I PSKKGWVEGQIPEDLN+NNL+LFYWFLA+LSILNFFH
Sbjct: 551 LSFGYYLSSIFVNVVNKVTRNIAPSKKGWVEGQIPEDLNHNNLNLFYWFLAILSILNFFH 610

Query: 543 YLYWASWYKYKAEEPIVQL-----------------NGGGGGEEGVPILQKDEERSVGDV 602
           YLYWASWYKYK EEPI +L                 NGGGGGEEG  I+Q  + R+ G+ 
Sbjct: 611 YLYWASWYKYKTEEPIAELDGGGGEDAPIVQDNRASNGGGGGEEGASIIQ--DNRAEGE- 670

Query: 603 TNHQNKGKEDVPILRENEERSGVDIQ-DHHSEGKE 620
                 G+E  PIL++ E   G D++ D +SE KE
Sbjct: 671 ------GEEGAPILQKEE---GSDVKADRYSEEKE 693

BLAST of Sed0025609 vs. ExPASy TrEMBL
Match: A0A1S4E0E0 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucumis melo OX=3656 GN=LOC103493841 PE=3 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 5.1e-285
Identity = 511/673 (75.93%), Postives = 566/673 (84.10%), Query Frame = 0

Query: 1   MQGTEEREIKMVDPKNEGKGGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLET 60
           ++GTEE++IK+VDPK+EGKGGFRATMFIF LLTFE+MGFVANM SLVQYFL VMHFDLET
Sbjct: 15  LRGTEEKDIKVVDPKDEGKGGFRATMFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLET 74

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKP 120
           AANTLTNF+GSAFLLSLLGGFLSDTYINRLTTCLIFG LE++AL++I VQAYSHDLLP P
Sbjct: 75  AANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIFGFLEVIALILITVQAYSHDLLPSP 134

Query: 121 ECPKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYML 180
            CPKDCV+GRIAFVFYTSLYLLA+GSGGVRGALPALGADQF+QKDPKEAKALGTFFNYML
Sbjct: 135 LCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYML 194

Query: 181 LSIVIGAAVGVTVIVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILR 240
           LS+V+GAAVGVTVIVWVAVNKAWYWGFFISA A  +GF++FAIGKPFYR+  PGQSPILR
Sbjct: 195 LSVVLGAAVGVTVIVWVAVNKAWYWGFFISALAALVGFIIFAIGKPFYRIQVPGQSPILR 254

Query: 241 IIEVIVVAFKNRGLSLPDRASELYELSDKCCMDTIDSKIAHTNQLRFFDKAAILAKDSEP 300
           +I+VIVVA KNR L LPD  +ELYE+SDK  MD+I  KI HTNQLRF DKAAI+ KD EP
Sbjct: 255 VIQVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIHYKIVHTNQLRFLDKAAIVPKDIEP 314

Query: 301 QAWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNFGHIQFP 360
           Q W VCSVTQVEEVKIITRM+PIFISTIIMNTCLAQLQTFSVEQGNT+IMDK+ GH QFP
Sbjct: 315 QPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTIIMDKSLGHFQFP 374

Query: 361 APSIPVIPLVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLV 420
           APSIPVIPLVFMA LIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAI+M VAGLV
Sbjct: 375 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMAVAGLV 434

Query: 421 EVKRRNQATKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480
           EVKRR+QA KHPDE ISLFWL+FQY IFG+ADMFTLVGLLEFFYKEAPVGMRSLSTSFT+
Sbjct: 435 EVKRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTF 494

Query: 481 LSLSFGYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNF 540
           LSL+ GYYLSSIFVN+VNKVT+ ITPSK+GWVEG IPEDLN+NNL+LFYWFLA+LS+LNF
Sbjct: 495 LSLALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSVLNF 554

Query: 541 FHYLYWASWYKYKAEEPIVQLNGGGGGEEGVPILQ---KDEERSVGDVTNHQNKGKEDVP 600
           FHYLYWASWYKYK EEPI +LN  GG     PI +   K+ E    +     NK  E+  
Sbjct: 555 FHYLYWASWYKYKTEEPIEELNSKGGETTKEPIAELNSKEGETMTKEPIAELNKQGEE-- 614

Query: 601 ILRENEERSGVDIQDHHSEGKEDIPILGDQKGVRNGVDGKDCQSKGKEGIPILGGEGERN 660
           I     E +G        EG+   P +    G        +   +G + +PIL  EG  N
Sbjct: 615 IKEPTAELNG------RGEGETKEPNVELNGGRETKEPITESNERGGDDVPILHKEG--N 674

Query: 661 GIDANDHHSEGKE 671
           G DA  HHSE KE
Sbjct: 675 GEDAKTHHSEEKE 677

BLAST of Sed0025609 vs. ExPASy TrEMBL
Match: A0A5A7ULM1 (Protein NRT1/ PTR FAMILY 4.5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold323G00540 PE=3 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 1.5e-284
Identity = 511/671 (76.15%), Postives = 564/671 (84.05%), Query Frame = 0

Query: 3   GTEEREIKMVDPKNEGKGGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLETAA 62
           GTEE++IK+VDPK+EGKGGFRATMFIF LLTFE+MGFVANM SLVQYFL VMHFDLETAA
Sbjct: 2   GTEEKDIKVVDPKDEGKGGFRATMFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAA 61

Query: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKPEC 122
           NTLTNF+GSAFLLSLLGGFLSDTYINRLTTCLIFG LE++AL++I VQAYSHDLLP P C
Sbjct: 62  NTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIFGFLEVIALILITVQAYSHDLLPSPLC 121

Query: 123 PKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYMLLS 182
           PKDCV+GRIAFVFYTSLYLLA+GSGGVRGALPALGADQF+QKDPKEAKALGTFFNYMLLS
Sbjct: 122 PKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLS 181

Query: 183 IVIGAAVGVTVIVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILRII 242
           +V+GAAVGVTVIVWVAVNKAWYWGFFISA A  +GF++FAIGKPFYR+  PGQSPILR+I
Sbjct: 182 VVLGAAVGVTVIVWVAVNKAWYWGFFISALAALVGFIIFAIGKPFYRIQVPGQSPILRVI 241

Query: 243 EVIVVAFKNRGLSLPDRASELYELSDKCCMDTIDSKIAHTNQLRFFDKAAILAKDSEPQA 302
           +VIVVA KNR L LPD  +ELYE+SDK  MD+I  KI HTNQLRF DKAAI+ KD EPQ 
Sbjct: 242 QVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQP 301

Query: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNFGHIQFPAP 362
           W VCSVTQVEEVKIITRM+PIFISTIIMNTCLAQLQTFSVEQGNT+IMDK+ GH QFPAP
Sbjct: 302 WNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTIIMDKSLGHFQFPAP 361

Query: 363 SIPVIPLVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLVEV 422
           SIPVIPLVFMA LIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAI+M VAGLVEV
Sbjct: 362 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMAVAGLVEV 421

Query: 423 KRRNQATKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482
           KRR+QA KHPDE ISLFWL+FQY IFG+ADMFTLVGLLEFFYKEAPVGMRSLSTSFT+LS
Sbjct: 422 KRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLS 481

Query: 483 LSFGYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNFFH 542
           L+ GYYLSSIFVN+VNKVT+ ITPSK+GWVEG IPEDLN+NNL+LFYWFLA+LS+LNFFH
Sbjct: 482 LALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFH 541

Query: 543 YLYWASWYKYKAEEPIVQLNGGGGGEEGVPILQ---KDEERSVGDVTNHQNKGKEDVPIL 602
           YLYWASWYKYK EEPI +LN  GG     PI +   K+ E    +     NK  E+  I 
Sbjct: 542 YLYWASWYKYKTEEPIEELNSRGGETTKEPIAELNSKEGETMTKEPIAELNKQGEE--IK 601

Query: 603 RENEERSGVDIQDHHSEGKEDIPILGDQKGVRNGVDGKDCQSKGKEGIPILGGEGERNGI 662
               E +G        EG+   P +    G        +   +G + +PIL  EG  NG 
Sbjct: 602 EPTAELNG------RGEGETKEPNVELNGGRETKEPITESNERGGDDVPILHKEG--NGE 661

Query: 663 DANDHHSEGKE 671
           DA  HHSE KE
Sbjct: 662 DAKTHHSEEKE 662

BLAST of Sed0025609 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 404.1 bits (1037), Expect = 2.3e-112
Identity = 230/547 (42.05%), Postives = 326/547 (59.60%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLETAANTLTNFLGSAFLLSLLG 79
           GG  A  F+ A+   EN+ F+AN  +LV Y    MH  L  +++ +T F+ +AFLL+LLG
Sbjct: 25  GGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLG 84

Query: 80  GFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKPECPK------DCVRGRIAF 139
           GFL+D + +     LI   +E L L+++ +QA    L+P P C        + V G  A 
Sbjct: 85  GFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMP-PPCKSSAALRCEVVGGSKAA 144

Query: 140 VFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYMLLSIVIGAAVGVTV 199
             +  LYL+++G GG++G+LP+ GA+QFD+  PK  K   TFFNY +  +  GA V VT 
Sbjct: 145 FLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTF 204

Query: 200 IVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILRIIEVIVVAFKNRG 259
           +VW+  NK W WGF +S  +  L  +VF +G  FY+   P  SP+  I +V++ A     
Sbjct: 205 VVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAA---SI 264

Query: 260 LSLPDRASELYELSDKCCMDTIDSKIAH--TNQLRFFDKAAILAKDSEPQAWKVCSVTQV 319
           +S   + S  +  S +   +  +   +   TN L   +KA    +      W  C+V QV
Sbjct: 265 VSCSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKA---IEGKTHHIWLECTVQQV 324

Query: 320 EEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNFGHIQFPAPSIPVIPLVF 379
           E+VKI+ +MLPIF  TI++N CLAQL T+SV Q  T  M++   +   P+ S+PV P+VF
Sbjct: 325 EDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFPVVF 384

Query: 380 MALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLVEVKRRNQATK- 439
           M +L P Y+ + +PFARK+T    GIT LQR+GVGLVLS ++M VA LVE+KR+  A + 
Sbjct: 385 MLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREA 444

Query: 440 -----HPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLSF 499
                     I+  W++ QY   G AD+FTL GLLEFF+ EAP  MRSL+TS +W SL+ 
Sbjct: 445 GLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLAL 504

Query: 500 GYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNFFHYLY 553
           GYYLSS+ V +VN+VTK  +  +  W    + E LN N LDLFYW + VLS++NF HYL+
Sbjct: 505 GYYLSSVMVPIVNRVTK--SAGQSPW----LGEKLNRNRLDLFYWLMCVLSVVNFLHYLF 556

BLAST of Sed0025609 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 404.1 bits (1037), Expect = 2.3e-112
Identity = 230/547 (42.05%), Postives = 326/547 (59.60%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLETAANTLTNFLGSAFLLSLLG 79
           GG  A  F+ A+   EN+ F+AN  +LV Y    MH  L  +++ +T F+ +AFLL+LLG
Sbjct: 29  GGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLG 88

Query: 80  GFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKPECPK------DCVRGRIAF 139
           GFL+D + +     LI   +E L L+++ +QA    L+P P C        + V G  A 
Sbjct: 89  GFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMP-PPCKSSAALRCEVVGGSKAA 148

Query: 140 VFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYMLLSIVIGAAVGVTV 199
             +  LYL+++G GG++G+LP+ GA+QFD+  PK  K   TFFNY +  +  GA V VT 
Sbjct: 149 FLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTF 208

Query: 200 IVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILRIIEVIVVAFKNRG 259
           +VW+  NK W WGF +S  +  L  +VF +G  FY+   P  SP+  I +V++ A     
Sbjct: 209 VVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAA---SI 268

Query: 260 LSLPDRASELYELSDKCCMDTIDSKIAH--TNQLRFFDKAAILAKDSEPQAWKVCSVTQV 319
           +S   + S  +  S +   +  +   +   TN L   +KA    +      W  C+V QV
Sbjct: 269 VSCSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKA---IEGKTHHIWLECTVQQV 328

Query: 320 EEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNFGHIQFPAPSIPVIPLVF 379
           E+VKI+ +MLPIF  TI++N CLAQL T+SV Q  T  M++   +   P+ S+PV P+VF
Sbjct: 329 EDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFPVVF 388

Query: 380 MALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLVEVKRRNQATK- 439
           M +L P Y+ + +PFARK+T    GIT LQR+GVGLVLS ++M VA LVE+KR+  A + 
Sbjct: 389 MLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREA 448

Query: 440 -----HPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLSF 499
                     I+  W++ QY   G AD+FTL GLLEFF+ EAP  MRSL+TS +W SL+ 
Sbjct: 449 GLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLAL 508

Query: 500 GYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNFFHYLY 553
           GYYLSS+ V +VN+VTK  +  +  W    + E LN N LDLFYW + VLS++NF HYL+
Sbjct: 509 GYYLSSVMVPIVNRVTK--SAGQSPW----LGEKLNRNRLDLFYWLMCVLSVVNFLHYLF 560

BLAST of Sed0025609 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 400.2 bits (1027), Expect = 3.3e-111
Identity = 230/546 (42.12%), Postives = 332/546 (60.81%), Query Frame = 0

Query: 14  PKNEGK-GGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLETAANTLTNFLGSA 73
           P N  K GG RA +F+  L  FE MG  A   +L+ Y +  MHF L  AAN +TNF+G+ 
Sbjct: 36  PSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTI 95

Query: 74  FLLSLLGGFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKPEC----PKDC-- 133
           F+ +LLGG+LSD ++    T +IFG +E+   ++++VQA+   L P P+C     + C  
Sbjct: 96  FIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKP-PKCNPLIDQTCEE 155

Query: 134 VRGRIAFVFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYMLLSIVIG 193
            +G  A +F+ +LYL+A+GSG V+  + A GADQF Q  PK++K L ++FN    +  +G
Sbjct: 156 AKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMG 215

Query: 194 AAVGVTVIVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILRIIEVIV 253
             + +T++VWV  +     GF +SA+A  +G +    G  ++R   P +S    I  VIV
Sbjct: 216 ELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIV 275

Query: 254 VAFKNRGLSLPDRASELYELSDKCCMDTIDSKIAHTNQLRFFDKAAILAKD--SEPQAWK 313
            A   R L+ P     L+            S + HT + RF DKA I  +D  ++   W+
Sbjct: 276 AAILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWR 335

Query: 314 VCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTL-IMDKNFGHIQFPAPS 373
           +C+VTQVE+VK +  ++PIF STI+ NT LAQLQTFSV+QG+++     N  HI  P  S
Sbjct: 336 LCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHI--PPAS 395

Query: 374 IPVIPLVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLVEVK 433
           +  IP + +  L+PLY+   VPFARK+T H SGI  L R+G+GL LS  SM  A ++E K
Sbjct: 396 LQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKK 455

Query: 434 RRNQATKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSL 493
           RR+ +       +S+FW++ Q+ IFG+++MFT VGL+EFFYK++  GM S   + T+ S 
Sbjct: 456 RRDSSVL-DGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSY 515

Query: 494 SFGYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNFFHY 550
           SFG+Y SS+ V+VVNK+T     S KGW+      DLN + LDLFYW LAVLS+LNF  Y
Sbjct: 516 SFGFYFSSVLVSVVNKITSTSVDS-KGWLG---ENDLNKDRLDLFYWLLAVLSLLNFLSY 573

BLAST of Sed0025609 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 396.4 bits (1017), Expect = 4.8e-110
Identity = 227/566 (40.11%), Postives = 328/566 (57.95%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLETAANTLTNFLGSAFLLSLLG 79
           GG  A  F+  +   EN+ ++AN  +LV Y    MH     +AN +TNF+G+AFLL+LLG
Sbjct: 27  GGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLG 86

Query: 80  GFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKPECPK---DCVRGRIAFVFY 139
           GFLSD + +     LI   +E L L+++ +QA +  L+P P C     + V G  A + +
Sbjct: 87  GFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMP-PSCDSPTCEEVSGSKAAMLF 146

Query: 140 TSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYMLLSIVIGAAVGVTVIVW 199
             LYL+A+G GG++G+L + GA+QFD+  PK  K   TFFNY +  +  GA V VT +VW
Sbjct: 147 VGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVW 206

Query: 200 VAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILRIIEVIVVAFK---NRG 259
           +  NK W WGF +S  A  +  ++F  G  FYR   P  SP+  I++V++ A     + G
Sbjct: 207 LEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSG 266

Query: 260 LSLPDRASELYELSDKCCM------------------DTIDSKIAHTNQLRFFDKAAILA 319
            S    AS     S+ C                    + +  +   TN L+  + AA   
Sbjct: 267 SSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAA--- 326

Query: 320 KDSEP-QAWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTLIMDKNF 379
            D +P      C+V QVE+VKI+ +MLPIF  TI++N CLAQL TFSV+Q  +  M+   
Sbjct: 327 -DEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAAS--MNTKI 386

Query: 380 GHIQFPAPSIPVIPLVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISM 439
           G ++ P  S+P+ P+VF+ +L P+Y+ + +PFARK T   +G+T LQR+GVGLVLS ++M
Sbjct: 387 GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAM 446

Query: 440 GVAGLVEVKRRNQA------TKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAP 499
            VA LVE+KR+  A             ++  W++ QY   G AD+FTL GLLE+F+ EAP
Sbjct: 447 AVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAP 506

Query: 500 VGMRSLSTSFTWLSLSFGYYLSSIFVNVVNKVTKKITPSKKGWVEGQIPEDLNYNNLDLF 555
             MRSL+TS +W SL+ GYYLSS+ V++VN +T   +     W+ G   + +N   LD F
Sbjct: 507 SSMRSLATSLSWASLAMGYYLSSVIVSIVNSITG--SSGNTPWLRG---KSINRYKLDYF 566

BLAST of Sed0025609 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 382.9 bits (982), Expect = 5.5e-106
Identity = 226/543 (41.62%), Postives = 329/543 (60.59%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFENMGFVANMVSLVQYFLMVMHFDLETAANTLTNFLGSAFLLSLLG 79
           GG RA +F+     FE M   A   +L+ Y    MHF L  +AN +TNF+G+ FLLSLLG
Sbjct: 40  GGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLG 99

Query: 80  GFLSDTYINRLTTCLIFGVLEILALVMIAVQAYSHDLLPKPECPK-----DCV--RGRIA 139
           GFLSD+Y+    T L+FGV+EI   ++++VQA+  +L P PEC        CV   G  A
Sbjct: 100 GFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRP-PECNMKSTTIHCVEANGYKA 159

Query: 140 FVFYTSLYLLAVGSGGVRGALPALGADQFDQKDPKEAKALGTFFNYMLLSIVIGAAVGVT 199
              YT+L L+A+GSG ++  + + GA+QF +KD    + L +FFN    +  +G  + +T
Sbjct: 160 ATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALT 219

Query: 200 VIVWVAVNKAWYWGFFISASATALGFVVFAIGKPFYRLHGPGQSPILRIIEVIVVAFKNR 259
           ++VWV  +     GF +SA+  A G +    G  FYR   P  S    I +V V A   R
Sbjct: 220 LLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKR 279

Query: 260 GLSLPDRASELYELSDKCCMDTIDSK-IAHTNQLRFFDKAAILAKDSEPQA-WKVCSVTQ 319
               P   + +++ S     D +  K + H+N+ RF DKA I  +    ++ W++C++ Q
Sbjct: 280 KQICPSNPNMVHQPS----TDLVRVKPLLHSNKFRFLDKACIKTQGKAMESPWRLCTIEQ 339

Query: 320 VEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTL--IMDKNFGHIQFPAPSIPVIP 379
           V +VKI+  ++PIF  TII NT LAQLQTFSV+QG+++   + K F   Q P  S+  IP
Sbjct: 340 VHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKTF---QIPPASLQAIP 399

Query: 380 LVFMALLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMGVAGLVEVKRRNQA 439
            + +   +PLYE  FVP ARK+T + SGI+ LQR+G GL L+  SM  A LVE KRR ++
Sbjct: 400 YIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRR-ES 459

Query: 440 TKHPDEQISLFWLSFQYAIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLSFGYY 499
               +  +S+FW++ Q+ IFG+++MFT VGL+EFFYK++   M+S  T+ T+ S SFG+Y
Sbjct: 460 FLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFY 519

Query: 500 LSSIFVNVVNKVTKKI-TPSKKGWVEGQIPEDLNYNNLDLFYWFLAVLSILNFFHYLYWA 551
           LSS+ V+ VN+VT    + +K+GW+      DLN + LD FYW LA LS +NFF+YL+W+
Sbjct: 520 LSSVLVSTVNRVTSSNGSGTKEGWLG---DNDLNKDRLDHFYWLLASLSFINFFNYLFWS 567