Sed0025551 (gene) Chayote v1

BLAST of Sed0025551 vs. NCBI nr
Match: KAG6593827.1 (Splicing factor U2AF 65 kDa subunit, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1351.3 bits (3496), Expect = 0.0e+00
Identity = 730/923 (79.09%), Postives = 801/923 (86.78%), Query Frame = 0

Query: 1   MSTYSCSKQHSRSSAKKSLYNSSDESAARTRPFSFEDIMLRRKNKGSGSNVEGVDAGSHS 60
           MSTYSCSKQ+SR+SAK+SLYNS+DESAARTRPFSFEDIMLRRKNK S +N +G D GSHS
Sbjct: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAAN-DGGDTGSHS 60

Query: 61  RRESIDKHVTANRESERHFRHSKGSSLDVQNPSLVESTKVSSRRKKEETLLKDKMVVRSD 120
           RRESIDKH+TANRESERHFRHS+GSSLD+QN  L ES K+SSRRKKEETLLKD MV+RSD
Sbjct: 61  RRESIDKHITANRESERHFRHSRGSSLDLQNLPLEESAKISSRRKKEETLLKDNMVIRSD 120

Query: 121 RNNHESRLTLMGKQKYDTNGKGKRQKYGQENLGWGKNEHRSRFEIENENGKRHSKDAGGK 180
           RNN+ES LTL+GK K DTNGK KRQKYGQENLGWGKN+ RSR +IENE GKRHS+DA GK
Sbjct: 121 RNNYESGLTLIGKLKRDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGK 180

Query: 181 DRHEDRAKGKIERESKRKYEHGEDDRNRDKHIPKRHDHDKHHDLENRERKETKISSSSHY 240
           DR EDR KGK ERESKRKY+HG+DDRNRD+HI K+HDH KHHDLE++ERKE KIS SSH+
Sbjct: 181 DRREDRGKGKSERESKRKYQHGDDDRNRDRHITKKHDHGKHHDLESKERKEAKISLSSHH 240

Query: 241 EDSKLKRRRKRSQDRESKHKRSGSRSPRPPKHSTKLARQNELPLDSHVNKSGRWISDSDK 300
           EDS+LK+RRKRS DR+SKHK+SGS SPRPPK STKLARQ ELPLDSHV KSGRW SDSD+
Sbjct: 241 EDSRLKKRRKRSLDRQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDR 300

Query: 301 TRDFTNSSSNQYKRHSGST-SGLGGYSPRKRRTESAVKTPSPVQSLEKKKEVSDLHPLEK 360
             DFTNSSS+QY+RHSGST SGLGGYSPRKRRTESAVKTP PV+S EKK E  DL P EK
Sbjct: 301 IGDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTP-PVRSPEKKNEGLDLLPAEK 360

Query: 361 VGLLPGSATSNFQLSNPIISLGISNDQSGGALFSPSMGKPLLEVSSNNLATKTNASLDLI 420
           VGL  GS TSNFQ SNP +S GISND S GALFS +MGK L  VSSNNLATKTNAS+D I
Sbjct: 361 VGLFSGSVTSNFQPSNPTVSSGISNDPSSGALFSLAMGKSLSGVSSNNLATKTNASVDSI 420

Query: 421 QLTQATRPVRRLYIENLPHSASEKAIIDYLNDFLISPGVNRTEGTQPCISCILHKDRGQA 480
           QLTQATRP+RRLYIENLPHS SEKAIID LN FL S GVN  EGTQPCISCI+HKDRGQA
Sbjct: 421 QLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQA 480

Query: 481 LVEFLTPDHASAALSFDGSDFSGSILKIRRPKDYVEIVTGDLDKSVVVVNEISDVVEDSP 540
           LVEFLTP+ ASAALSFDGSDFSGSIL+IRRPKDYVE+VTGDL+KSV VVN+ISDVVEDSP
Sbjct: 481 LVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVTGDLEKSVAVVNKISDVVEDSP 540

Query: 541 NKIIVAGISNRISSEMLRDIVTAFGPLKAYHFEINNELNEPCAFLEYDDQSVMPKACAGL 600
           NKIIVAGISNRISSEML++IV AFGPLKAYHFEINN+LNEPCAFLEY DQSVMPKACAGL
Sbjct: 541 NKIIVAGISNRISSEMLKNIVAAFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGL 600

Query: 601 NGLKIGGGVLKVFPAVPFAPLERNGWQPCYVIPEHVKPLLQQPTAVLKVNNVFNADVLSV 660
           NG+KIGG VLKVFPAVP A LERNG QPCY IPEHVKPLLQQPT VLKV NVFNADVL +
Sbjct: 601 NGMKIGGQVLKVFPAVPIASLERNGCQPCYGIPEHVKPLLQQPTVVLKV-NVFNADVLPL 660

Query: 661 LSESDIEEVLEDIRFECARFGTVKSMNVAKPCRSCVNAEEEYKNNSDATDVVVKHEIQEI 720
           LSESDI EVLEDIRFECARFGTVKSMNVAKPC SC++AEEEYK+  D TDV +KHEIQEI
Sbjct: 661 LSESDINEVLEDIRFECARFGTVKSMNVAKPCTSCISAEEEYKSICDVTDVEIKHEIQEI 720

Query: 721 SPIVISRNDNGLEDNKAKLDNCPDSTYQEQGDCPMNDRHQNEVVEDKLCQMGSNDATHF- 780
           S IVISRND   +      DNCPD TYQE+G+ P N RH+NEVVEDKLC MG  DAT F 
Sbjct: 721 STIVISRNDTDHD------DNCPDDTYQEKGNYPHNGRHKNEVVEDKLCHMGIKDATCFE 780

Query: 781 NVACEGASETITQGLDDQRSSPGNELHDAKAAKIIETDETGSDVKSVLLGDSTTVMADSE 840
           +V C+ ASETI QG  D++SSPGNELHD K AKII   ETGSD K VLLGDSTTV+AD E
Sbjct: 781 DVVCKSASETIPQGYCDKQSSPGNELHDVKVAKII---ETGSDEKPVLLGDSTTVVADDE 840

Query: 841 KKVLNGLDPIVGTDCGDTSEKSKEKDPNNNLESLYASGSVFVEFGRMEASYMAAHAFHGR 900
           KKVL+G+DP+V TD  DTSEK ++K+ NNNLES +A GSVFVEFGR+EAS MAAH+ HGR
Sbjct: 841 KKVLHGMDPMVRTD-SDTSEKREKKERNNNLESSFAVGSVFVEFGRLEASCMAAHSLHGR 900

Query: 901 IYDGREISVEFFPRDLYHKRFPK 922
           IYDG+EISVE+ P DLYH+RFPK
Sbjct: 901 IYDGQEISVEYIPHDLYHRRFPK 910

BLAST of Sed0025551 vs. NCBI nr
Match: XP_023514084.1 (splicing factor U2af large subunit A [Cucurbita pepo subsp. pepo] >XP_023514085.1 splicing factor U2af large subunit A [Cucurbita pepo subsp. pepo] >XP_023514086.1 splicing factor U2af large subunit A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1347.0 bits (3485), Expect = 0.0e+00
Identity = 728/923 (78.87%), Postives = 800/923 (86.67%), Query Frame = 0

Query: 1   MSTYSCSKQHSRSSAKKSLYNSSDESAARTRPFSFEDIMLRRKNKGSGSNVEGVDAGSHS 60
           MSTYSCSKQ+SR+SAK+SLYNS+DESAARTRPFSFEDIMLRRKNK S +N +G D GSHS
Sbjct: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAAN-DGGDTGSHS 60

Query: 61  RRESIDKHVTANRESERHFRHSKGSSLDVQNPSLVESTKVSSRRKKEETLLKDKMVVRSD 120
           RRESIDKH+TANRESERHFRHS+GSSLDVQN  L ES ++SSRRKKEETLLKD MV+RSD
Sbjct: 61  RRESIDKHITANRESERHFRHSRGSSLDVQNLPLEESARISSRRKKEETLLKDNMVIRSD 120

Query: 121 RNNHESRLTLMGKQKYDTNGKGKRQKYGQENLGWGKNEHRSRFEIENENGKRHSKDAGGK 180
           RNN+ S LTL+GK K+DTNGK KRQKYGQENLGWGKN+ RSR +IENE GKRHS+DA GK
Sbjct: 121 RNNYGSGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGK 180

Query: 181 DRHEDRAKGKIERESKRKYEHGEDDRNRDKHIPKRHDHDKHHDLENRERKETKISSSSHY 240
           DR EDR KGK ERESKRKY+HG+DDRNRD+HI K+HDH KHHDLE++ERKE KI  SSH+
Sbjct: 181 DRREDRGKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHHDLESKERKEAKILLSSHH 240

Query: 241 EDSKLKRRRKRSQDRESKHKRSGSRSPRPPKHSTKLARQNELPLDSHVNKSGRWISDSDK 300
           EDS+LK+RRKRS D +SKHK+SGS SPRPPK STKLARQ ELPLDSHV KSGRW SDSD+
Sbjct: 241 EDSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDR 300

Query: 301 TRDFTNSSSNQYKRHSGST-SGLGGYSPRKRRTESAVKTPSPVQSLEKKKEVSDLHPLEK 360
             DFTNSSS+QY+RHSGST SGLGGYSPRKRRTESAVKTP PV+S EKK E  DL P EK
Sbjct: 301 IGDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTP-PVRSPEKKNEGLDLLPAEK 360

Query: 361 VGLLPGSATSNFQLSNPIISLGISNDQSGGALFSPSMGKPLLEVSSNNLATKTNASLDLI 420
           VGL  GS TSNFQ SNP +S GISND SGGALFS +MGK L  VSSNNLATKTNAS+D I
Sbjct: 361 VGLFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASVDSI 420

Query: 421 QLTQATRPVRRLYIENLPHSASEKAIIDYLNDFLISPGVNRTEGTQPCISCILHKDRGQA 480
           QLTQATRP+RRLYIENLPHS SEKAIID LN FL S GVN  EGTQPCISCI+HKDRGQA
Sbjct: 421 QLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQA 480

Query: 481 LVEFLTPDHASAALSFDGSDFSGSILKIRRPKDYVEIVTGDLDKSVVVVNEISDVVEDSP 540
           LVEFLTP+ ASAALSFDGSDFSGSIL+IRRPKDYVE+VTGDL+KSV VVN+ISDVVEDSP
Sbjct: 481 LVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVTGDLEKSVAVVNKISDVVEDSP 540

Query: 541 NKIIVAGISNRISSEMLRDIVTAFGPLKAYHFEINNELNEPCAFLEYDDQSVMPKACAGL 600
           NKIIVAGISNRISSEML++IV AFGPLKAYHFEINN+LNEPCAFLEY DQSVMPKACAGL
Sbjct: 541 NKIIVAGISNRISSEMLKNIVAAFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGL 600

Query: 601 NGLKIGGGVLKVFPAVPFAPLERNGWQPCYVIPEHVKPLLQQPTAVLKVNNVFNADVLSV 660
           NG+KIGG VLKVFPAVP A LERNG QPCY IPEHVKPLLQQPT VLKV NVFNADVL +
Sbjct: 601 NGMKIGGQVLKVFPAVPIASLERNGCQPCYGIPEHVKPLLQQPTVVLKV-NVFNADVLPL 660

Query: 661 LSESDIEEVLEDIRFECARFGTVKSMNVAKPCRSCVNAEEEYKNNSDATDVVVKHEIQEI 720
           LSESDI EVLEDIRFECARFGTVKSMNVAKPC SCV+AEEEYK+  D TDV +KHEIQEI
Sbjct: 661 LSESDINEVLEDIRFECARFGTVKSMNVAKPCTSCVSAEEEYKSICDVTDVEIKHEIQEI 720

Query: 721 SPIVISRNDNGLEDNKAKLDNCPDSTYQEQGDCPMNDRHQNEVVEDKLCQMGSNDATHF- 780
           S IVISRNDN  +      DNCPD+TYQEQG+ P N RH+NEVVEDKLC MG  DAT F 
Sbjct: 721 STIVISRNDNDHD------DNCPDATYQEQGNYPHNGRHKNEVVEDKLCHMGIKDATCFE 780

Query: 781 NVACEGASETITQGLDDQRSSPGNELHDAKAAKIIETDETGSDVKSVLLGDSTTVMADSE 840
           ++ C+ ASETI +G  D++SSPGNELHD K AKII   ETGSD K VLLGDSTTV+AD E
Sbjct: 781 DIVCKSASETIPRGCCDKQSSPGNELHDVKVAKII---ETGSDEKPVLLGDSTTVVADDE 840

Query: 841 KKVLNGLDPIVGTDCGDTSEKSKEKDPNNNLESLYASGSVFVEFGRMEASYMAAHAFHGR 900
           KKVL+G+DP+V TD  DTSE  ++K+ NNNLES +A GSVFVEFGR+EAS MAAH+ HGR
Sbjct: 841 KKVLHGMDPMVRTD-SDTSENREKKERNNNLESSFAVGSVFVEFGRLEASCMAAHSLHGR 900

Query: 901 IYDGREISVEFFPRDLYHKRFPK 922
           IYDG+EISVE+ P DLYHKRF K
Sbjct: 901 IYDGQEISVEYIPHDLYHKRFSK 910

BLAST of Sed0025551 vs. NCBI nr
Match: XP_022964139.1 (splicing factor U2af large subunit A-like [Cucurbita moschata] >XP_022964140.1 splicing factor U2af large subunit A-like [Cucurbita moschata] >XP_022964141.1 splicing factor U2af large subunit A-like [Cucurbita moschata])

HSP 1 Score: 1341.3 bits (3470), Expect = 0.0e+00
Identity = 727/923 (78.76%), Postives = 797/923 (86.35%), Query Frame = 0

Query: 1   MSTYSCSKQHSRSSAKKSLYNSSDESAARTRPFSFEDIMLRRKNKGSGSNVEGVDAGSHS 60
           MSTYSCSKQ+SR+SAK+SLYNS+DESAARTRPFSFEDIMLRRKNK S +N +G D GSHS
Sbjct: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAAN-DGGDTGSHS 60

Query: 61  RRESIDKHVTANRESERHFRHSKGSSLDVQNPSLVESTKVSSRRKKEETLLKDKMVVRSD 120
           RRESIDKH+TAN ESERHFRHS+GSSLD+QN  L ES K+SSRRKKEETLLKD MV+RSD
Sbjct: 61  RRESIDKHITANLESERHFRHSRGSSLDLQNLPLEESAKISSRRKKEETLLKDNMVIRSD 120

Query: 121 RNNHESRLTLMGKQKYDTNGKGKRQKYGQENLGWGKNEHRSRFEIENENGKRHSKDAGGK 180
           RNN+ES LTL+GK K DTNGK KRQKYGQENLGWGKN+ RSR +IENE GKR S+DA GK
Sbjct: 121 RNNYESGLTLIGKLKRDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRRSRDAAGK 180

Query: 181 DRHEDRAKGKIERESKRKYEHGEDDRNRDKHIPKRHDHDKHHDLENRERKETKISSSSHY 240
           DR EDR KGK ERESKRKY+HG+DDRNRD+HI K+HDH KHHDLE++ERKE KIS SSH+
Sbjct: 181 DRREDRGKGKSERESKRKYQHGDDDRNRDRHITKKHDHGKHHDLESKERKEAKISLSSHH 240

Query: 241 EDSKLKRRRKRSQDRESKHKRSGSRSPRPPKHSTKLARQNELPLDSHVNKSGRWISDSDK 300
           EDS+LK+RRKRS D +SKHK+SGS SPRPPK STKLARQ ELPLDSHV KSGRW SDSD+
Sbjct: 241 EDSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDR 300

Query: 301 TRDFTNSSSNQYKRHSGST-SGLGGYSPRKRRTESAVKTPSPVQSLEKKKEVSDLHPLEK 360
             DFTNSSS+QY+RHSGST SGLGGYSPRKRRTESAVKTP PV+S EKK E  DL P E 
Sbjct: 301 IGDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTP-PVRSPEKKNEGLDLLPAET 360

Query: 361 VGLLPGSATSNFQLSNPIISLGISNDQSGGALFSPSMGKPLLEVSSNNLATKTNASLDLI 420
           VGL  GS TSNFQ SNP +S GISND SGGALFS +MGK L  VSSNNLATKTNAS+D I
Sbjct: 361 VGLFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASIDSI 420

Query: 421 QLTQATRPVRRLYIENLPHSASEKAIIDYLNDFLISPGVNRTEGTQPCISCILHKDRGQA 480
           QLTQATRP+RRLYIENLPHS SEKAIID LN FL S GVN  EGTQPCISCI+HKDRGQA
Sbjct: 421 QLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQA 480

Query: 481 LVEFLTPDHASAALSFDGSDFSGSILKIRRPKDYVEIVTGDLDKSVVVVNEISDVVEDSP 540
           LVEFLTP+ ASAALSFDGSDFSGSIL+IRRPKDYVE+VTGDL+KSV VVN+ISDVVEDSP
Sbjct: 481 LVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVTGDLEKSVAVVNKISDVVEDSP 540

Query: 541 NKIIVAGISNRISSEMLRDIVTAFGPLKAYHFEINNELNEPCAFLEYDDQSVMPKACAGL 600
           NKIIVAGISNRISSEML++IV AFGPLKAYHFEINN+LNEPCAFLEY DQSVMPKACAGL
Sbjct: 541 NKIIVAGISNRISSEMLKNIVAAFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGL 600

Query: 601 NGLKIGGGVLKVFPAVPFAPLERNGWQPCYVIPEHVKPLLQQPTAVLKVNNVFNADVLSV 660
           NG+KIGG VLKVFPAVP A LERNG QPCY IPEHVKPLLQQPT VLKV NVFNADVL +
Sbjct: 601 NGMKIGGQVLKVFPAVPIASLERNGCQPCYGIPEHVKPLLQQPTVVLKV-NVFNADVLPL 660

Query: 661 LSESDIEEVLEDIRFECARFGTVKSMNVAKPCRSCVNAEEEYKNNSDATDVVVKHEIQEI 720
           LSESDI EVLEDIRFECARFGTVKSMNVAK C SCV+AEEEYK+  D TDV +KHEIQEI
Sbjct: 661 LSESDINEVLEDIRFECARFGTVKSMNVAKSCTSCVSAEEEYKSICDVTDVEIKHEIQEI 720

Query: 721 SPIVISRNDNGLEDNKAKLDNCPDSTYQEQGDCPMNDRHQNEVVEDKLCQMGSNDATHF- 780
           S IVISRND   +      DNCPD TYQEQG+ P N RH+NEVVEDKLC MG  DAT F 
Sbjct: 721 STIVISRNDTDHD------DNCPDDTYQEQGNYPHNGRHKNEVVEDKLCHMGIKDATCFE 780

Query: 781 NVACEGASETITQGLDDQRSSPGNELHDAKAAKIIETDETGSDVKSVLLGDSTTVMADSE 840
           +V C+ ASETI +G  D++SSPGNELHD K AKII   ETGSD K VLLGDSTTV+AD E
Sbjct: 781 DVVCKSASETIPRGCCDKQSSPGNELHDVKVAKII---ETGSDEKPVLLGDSTTVVADDE 840

Query: 841 KKVLNGLDPIVGTDCGDTSEKSKEKDPNNNLESLYASGSVFVEFGRMEASYMAAHAFHGR 900
           KKVL+G+DP+V TD  DTSEK ++K+ NNNLES +A GSVFVEFGR+EAS MAAH+ HGR
Sbjct: 841 KKVLHGMDPMVRTD-SDTSEKREKKERNNNLESSFAVGSVFVEFGRLEASCMAAHSLHGR 900

Query: 901 IYDGREISVEFFPRDLYHKRFPK 922
           IYDG+EISVE+ P DLYH+RFPK
Sbjct: 901 IYDGQEISVEYIPHDLYHRRFPK 910

BLAST of Sed0025551 vs. NCBI nr
Match: KAG7026156.1 (Splicing factor U2AF 65 kDa subunit [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1338.2 bits (3462), Expect = 0.0e+00
Identity = 730/951 (76.76%), Postives = 802/951 (84.33%), Query Frame = 0

Query: 1   MSTYSCSKQHSRSSAKKSLYNSSDESAARTRPFSFEDIMLRRKNKGSGSNVEGVDAGSHS 60
           MSTYSCSKQ+SR+SAK+SLYNS+DESAARTRPFSFEDIMLRRKNK S +N +G D GSHS
Sbjct: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAAN-DGGDTGSHS 60

Query: 61  RRESIDKHVTANRESERHFRHSKGSSLDVQNPSLVESTKVSSRRKKEETLLKDKMVVRSD 120
           RRESIDKH+TANRESERHFRHS+GSSLD+QN  L ES K+SSRRKKEETLLKD MV+RSD
Sbjct: 61  RRESIDKHITANRESERHFRHSRGSSLDLQNLPLEESAKISSRRKKEETLLKDNMVIRSD 120

Query: 121 RNNHESRLTLMGKQKYDTNGKGKRQKYGQENLGWGKNEHRSRFEIENENGKRHSKDAGGK 180
           RNN+ES LTL+GK K DTNGK KRQKYGQENLGWGKN+ RSR +IENE GKRHS+DA GK
Sbjct: 121 RNNYESGLTLIGKLKRDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGK 180

Query: 181 DRHEDRAKGKIERESKRKYEHGEDDRNRDKHIPKRHDHDKHHDLENRERKETKISSSSHY 240
           DR EDR KGK ERESKRKY+HG+DDRNRD+HI K+HDH KHHDLE++ERKE KIS SSH+
Sbjct: 181 DRREDRGKGKSERESKRKYQHGDDDRNRDRHITKKHDHGKHHDLESKERKEAKISLSSHH 240

Query: 241 EDSKLKRRRKRSQDRESKHKRSGSRSPRPPKHSTKLARQNELPLDSHVNKSGRWISDSDK 300
           EDS+LK+RRKRS DR+SKHK+SGS SPRPPK STKLARQ ELPLDSHV KSGRW SDSD+
Sbjct: 241 EDSRLKKRRKRSLDRQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDR 300

Query: 301 TRDFTNSSSNQYKRHSGST-SGLGGYSPRKRRTESAVKTPSPVQSLEKKKEVSDLHPLEK 360
             DFTNSSS+QY+RHSGST SGLGGYSPRKRRTESAVKTP PV+S EKK E  DL P EK
Sbjct: 301 IGDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTP-PVRSPEKKNEGLDLLPAEK 360

Query: 361 VGLLPGSATSNFQLSNPIISLGISNDQSGGALFSPSMGKPLLEVSSNNLATKTNASLDLI 420
           VGL  GS TSNFQ SNP +S GISND S GALFS +MGK L  VSSNNLATKTNAS+D I
Sbjct: 361 VGLFSGSVTSNFQPSNPTVSSGISNDPSSGALFSLAMGKSLSGVSSNNLATKTNASVDSI 420

Query: 421 QLTQATRPVRRLYIENLPHSASEKAIIDYLNDFLISPGVNRTEGTQPCISCILHKDRGQA 480
           QLTQATRP+RRLYIENLPHS SEKAIID LN FL S GVN  EGTQPCISCI+HKDRGQA
Sbjct: 421 QLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQA 480

Query: 481 LVEFLTPDHASAALSFDGSDFSGSILKIRRPKDYVEIVTGDLDKSVVVVNEISDVVEDSP 540
           LVEFLTP+ ASAALSFDGSDFSGSIL+IRRPKDYVE+VTGDL+KSV VVN+ISDVVEDSP
Sbjct: 481 LVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVTGDLEKSVAVVNKISDVVEDSP 540

Query: 541 NKIIVAGISNRISSEMLRDIVTAFGPLKAYHFEINNELNEPCAFLE-------------- 600
           NKIIVAGISNRISSEML++IV AFGPLKAYHFEINN+LNEPCAFLE              
Sbjct: 541 NKIIVAGISNRISSEMLKNIVAAFGPLKAYHFEINNDLNEPCAFLELYINSPKASIYRHI 600

Query: 601 --------------YDDQSVMPKACAGLNGLKIGGGVLKVFPAVPFAPLERNGWQPCYVI 660
                         Y DQSVMPKACAGLNG+KIGG VLKVFPAVP A LERNG QPCY I
Sbjct: 601 IAHGNPLIISSRLFYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLERNGCQPCYGI 660

Query: 661 PEHVKPLLQQPTAVLKVNNVFNADVLSVLSESDIEEVLEDIRFECARFGTVKSMNVAKPC 720
           PEHVKPLLQQPT VLKV NVFNADVL +LSESDI EVLEDIRFECARFGTVKSMNVAKPC
Sbjct: 661 PEHVKPLLQQPTVVLKV-NVFNADVLPLLSESDINEVLEDIRFECARFGTVKSMNVAKPC 720

Query: 721 RSCVNAEEEYKNNSDATDVVVKHEIQEISPIVISRNDNGLEDNKAKLDNCPDSTYQEQGD 780
            SC++AEEEYK+  D TDV +KHEIQEIS IVISRND   +      DNCPD TYQEQG+
Sbjct: 721 TSCISAEEEYKSICDVTDVEIKHEIQEISTIVISRNDTDHD------DNCPDDTYQEQGN 780

Query: 781 CPMNDRHQNEVVEDKLCQMGSNDATHF-NVACEGASETITQGLDDQRSSPGNELHDAKAA 840
            P N RH+NEVVEDKLC MG  DAT+F +V C+ ASETI +G  D++SSPGNELHD K A
Sbjct: 781 YPHNGRHKNEVVEDKLCHMGIKDATYFEDVVCKSASETIPRGCCDKQSSPGNELHDVKVA 840

Query: 841 KIIETDETGSDVKSVLLGDSTTVMADSEKKVLNGLDPIVGTDCGDTSEKSKEKDPNNNLE 900
           KII   ETGSD K VLLGDSTTV+AD EKKVL+G+DP+V TD  DTSEK ++K+ NNNLE
Sbjct: 841 KII---ETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTD-SDTSEKREKKERNNNLE 900

Query: 901 SLYASGSVFVEFGRMEASYMAAHAFHGRIYDGREISVEFFPRDLYHKRFPK 922
           S +A GSVFVEFGR+EAS MAAH+ HGRIYDG+EISVE+ P DLYH+RFPK
Sbjct: 901 SSFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHRRFPK 938

BLAST of Sed0025551 vs. NCBI nr
Match: XP_023000460.1 (splicing factor U2af large subunit A-like [Cucurbita maxima] >XP_023000461.1 splicing factor U2af large subunit A-like [Cucurbita maxima] >XP_023000462.1 splicing factor U2af large subunit A-like [Cucurbita maxima])

HSP 1 Score: 1328.2 bits (3436), Expect = 0.0e+00
Identity = 720/923 (78.01%), Postives = 793/923 (85.92%), Query Frame = 0

Query: 1   MSTYSCSKQHSRSSAKKSLYNSSDESAARTRPFSFEDIMLRRKNKGSGSNVEGVDAGSHS 60
           MSTYSCSKQ+SR+SAK+ LYNS+DESAARTRPFSFEDIMLRRKNK S +  +G D  S S
Sbjct: 1   MSTYSCSKQYSRTSAKQCLYNSNDESAARTRPFSFEDIMLRRKNKESAA-TDGGDTSSQS 60

Query: 61  RRESIDKHVTANRESERHFRHSKGSSLDVQNPSLVESTKVSSRRKKEETLLKDKMVVRSD 120
           RRESIDKH+TANRESERHFRHS+GSSLDVQN  L ES K+SSRRKKEETLLKD MV+RSD
Sbjct: 61  RRESIDKHITANRESERHFRHSRGSSLDVQNLPLEESAKISSRRKKEETLLKDNMVIRSD 120

Query: 121 RNNHESRLTLMGKQKYDTNGKGKRQKYGQENLGWGKNEHRSRFEIENENGKRHSKDAGGK 180
           RNN+ES LTL+GK K+DTNGK KRQKYGQENLGWGKN+ RSR +IENE GKRHS+DA GK
Sbjct: 121 RNNYESGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGK 180

Query: 181 DRHEDRAKGKIERESKRKYEHGEDDRNRDKHIPKRHDHDKHHDLENRERKETKISSSSHY 240
           DR E+R KGK ERESKRKY+HG+DDRNRD+HI K+HDH KHHDLE++ERKE KIS SSH+
Sbjct: 181 DRRENRGKGKSERESKRKYQHGDDDRNRDRHITKKHDHGKHHDLESKERKEAKISLSSHH 240

Query: 241 EDSKLKRRRKRSQDRESKHKRSGSRSPRPPKHSTKLARQNELPLDSHVNKSGRWISDSDK 300
           EDS+LK+RRKRS DR+SKHK+SGS SPRPPK STKLARQ ELPLDSHV KSGRW SDSD+
Sbjct: 241 EDSRLKKRRKRSLDRQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDR 300

Query: 301 TRDFTNSSSNQYKRHSGST-SGLGGYSPRKRRTESAVKTPSPVQSLEKKKEVSDLHPLEK 360
             DFTNSSS+QY+RHSG T SGLGGYSPRKRRTESAVKTP PV+S EKK E  DL P EK
Sbjct: 301 IGDFTNSSSSQYRRHSGLTRSGLGGYSPRKRRTESAVKTP-PVRSPEKKNEGLDLLPAEK 360

Query: 361 VGLLPGSATSNFQLSNPIISLGISNDQSGGALFSPSMGKPLLEVSSNNLATKTNASLDLI 420
           VGL  GS TSNFQ SNP +S GISND SGGALFS + GK L  +SSNNLATKTNAS D I
Sbjct: 361 VGLFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLTTGKSLSGISSNNLATKTNASFDSI 420

Query: 421 QLTQATRPVRRLYIENLPHSASEKAIIDYLNDFLISPGVNRTEGTQPCISCILHKDRGQA 480
           QLTQATRP+RRLYIENLPHS SEKAIID LN FL S GVN  EGTQPCISCI+HKDRGQA
Sbjct: 421 QLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQA 480

Query: 481 LVEFLTPDHASAALSFDGSDFSGSILKIRRPKDYVEIVTGDLDKSVVVVNEISDVVEDSP 540
           LVEFLTP+ ASAALSFDGSDFSGSIL+IRRPKDYVE+VTGDLDKSV VVN+I+DVVEDSP
Sbjct: 481 LVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVTGDLDKSVAVVNKINDVVEDSP 540

Query: 541 NKIIVAGISNRISSEMLRDIVTAFGPLKAYHFEINNELNEPCAFLEYDDQSVMPKACAGL 600
           NKII+AGISNRISSEML++IV AFGPLKAYHFEINN+LNEPCAFLEY DQSVMPKACAGL
Sbjct: 541 NKIIIAGISNRISSEMLKNIVAAFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGL 600

Query: 601 NGLKIGGGVLKVFPAVPFAPLERNGWQPCYVIPEHVKPLLQQPTAVLKVNNVFNADVLSV 660
           NG+KIGG VLKVFPAVP A LERNG QPCY IPEHVKPLLQQPT VLKV NVFNADVL +
Sbjct: 601 NGMKIGGEVLKVFPAVPIASLERNGCQPCYGIPEHVKPLLQQPTVVLKV-NVFNADVLPL 660

Query: 661 LSESDIEEVLEDIRFECARFGTVKSMNVAKPCRSCVNAEEEYKNNSDATDVVVKHEIQEI 720
           LSESDI EVLEDIRFECARFGTVKSMNVAKPC SCV+AEEEY++  D TDV +KHEIQEI
Sbjct: 661 LSESDINEVLEDIRFECARFGTVKSMNVAKPCTSCVSAEEEYQSICDITDVEIKHEIQEI 720

Query: 721 SPIVISRNDNGLEDNKAKLDNCPDSTYQEQGDCPMNDRHQNEVVEDKLCQMGSNDATHF- 780
           S IVISRND   +      DNC D TYQEQG+ P N RH+NEVVEDKLC MG  DAT F 
Sbjct: 721 STIVISRNDTDHD------DNCLDYTYQEQGNYPHNGRHKNEVVEDKLCHMGIKDATCFE 780

Query: 781 NVACEGASETITQGLDDQRSSPGNELHDAKAAKIIETDETGSDVKSVLLGDSTTVMADSE 840
           +V C+ A ETI +G  D++SSPGNELH  K AKII   ETGSD K VLLGDS+TV+AD E
Sbjct: 781 DVVCKSALETIPRGCCDKQSSPGNELHGVKVAKII---ETGSDEKPVLLGDSSTVVADDE 840

Query: 841 KKVLNGLDPIVGTDCGDTSEKSKEKDPNNNLESLYASGSVFVEFGRMEASYMAAHAFHGR 900
           KKVL+G+DP+V TD  DTSEK ++K+ NNNLES +A GSVFVEFGR+EAS MAAH+ HGR
Sbjct: 841 KKVLHGMDPMVRTD-SDTSEKREKKERNNNLESSFAVGSVFVEFGRLEASCMAAHSLHGR 900

Query: 901 IYDGREISVEFFPRDLYHKRFPK 922
           IYDG+EISVE+ P DLYHKRFPK
Sbjct: 901 IYDGQEISVEYIPHDLYHKRFPK 910

BLAST of Sed0025551 vs. ExPASy Swiss-Prot
Match: P26368 (Splicing factor U2AF 65 kDa subunit OS=Homo sapiens OX=9606 GN=U2AF2 PE=1 SV=4)

HSP 1 Score: 155.6 bits (392), Expect = 2.7e-36
Identity = 94/282 (33.33%), Postives = 159/282 (56.38%), Query Frame = 0

Query: 422 TQATRPVRRLYIENLPHSASEKAIIDYLNDFLISPGVNRTEGTQPCISCILHKDRGQALV 481
           +Q TR  RRLY+ N+P   +E+A++D+ N  +   G+ +  G  P ++  +++D+  A +
Sbjct: 142 SQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPG-NPVLAVQINQDKNFAFL 201

Query: 482 EFLTPDHASAALSFDGSDFSGSILKIRRPKDYVEIVTGDLDKSVVVVNEISDVVEDSPNK 541
           EF + D  + A++FDG  F G  LKIRRP DY  +     + SV V   +S VV DS +K
Sbjct: 202 EFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHK 261

Query: 542 IIVAGISNRISSEMLRDIVTAFGPLKAYHF--EINNELNEPCAFLEYDDQSVMPKACAGL 601
           + + G+ N ++ + +++++T+FGPLKA++   +    L++  AF EY D +V  +A AGL
Sbjct: 262 LFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGL 321

Query: 602 NGLKIGGGVLKVFPAVPFA--------PLERNGWQPCYVIPEHVKPLLQ---QPTAVLKV 661
           NG+++G   L V  A   A        P   N       +P  +   +Q    PT VL +
Sbjct: 322 NGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCL 381

Query: 662 NNVFNADVLSVLSESDIEEVLEDIRFECARFGTVKSMNVAKP 691
            N+   + L  L + + EE++ED+R EC+++G VKS+ + +P
Sbjct: 382 MNMVLPEEL--LDDEEYEEIVEDVRDECSKYGLVKSIEIPRP 420

BLAST of Sed0025551 vs. ExPASy Swiss-Prot
Match: P26369 (Splicing factor U2AF 65 kDa subunit OS=Mus musculus OX=10090 GN=U2af2 PE=1 SV=3)

HSP 1 Score: 155.6 bits (392), Expect = 2.7e-36
Identity = 94/282 (33.33%), Postives = 159/282 (56.38%), Query Frame = 0

Query: 422 TQATRPVRRLYIENLPHSASEKAIIDYLNDFLISPGVNRTEGTQPCISCILHKDRGQALV 481
           +Q TR  RRLY+ N+P   +E+A++D+ N  +   G+ +  G  P ++  +++D+  A +
Sbjct: 142 SQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPG-NPVLAVQINQDKNFAFL 201

Query: 482 EFLTPDHASAALSFDGSDFSGSILKIRRPKDYVEIVTGDLDKSVVVVNEISDVVEDSPNK 541
           EF + D  + A++FDG  F G  LKIRRP DY  +     + SV V   +S VV DS +K
Sbjct: 202 EFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHK 261

Query: 542 IIVAGISNRISSEMLRDIVTAFGPLKAYHF--EINNELNEPCAFLEYDDQSVMPKACAGL 601
           + + G+ N ++ + +++++T+FGPLKA++   +    L++  AF EY D +V  +A AGL
Sbjct: 262 LFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGL 321

Query: 602 NGLKIGGGVLKVFPAVPFA--------PLERNGWQPCYVIPEHVKPLLQ---QPTAVLKV 661
           NG+++G   L V  A   A        P   N       +P  +   +Q    PT VL +
Sbjct: 322 NGMQLGDKKLLVQRASVGAKNATLVSLPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCL 381

Query: 662 NNVFNADVLSVLSESDIEEVLEDIRFECARFGTVKSMNVAKP 691
            N+   + L  L + + EE++ED+R EC+++G VKS+ + +P
Sbjct: 382 MNMVLPEEL--LDDEEYEEIVEDVRDECSKYGLVKSIEIPRP 420

BLAST of Sed0025551 vs. ExPASy Swiss-Prot
Match: Q24562 (Splicing factor U2AF 50 kDa subunit OS=Drosophila melanogaster OX=7227 GN=U2af50 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 5.6e-34
Identity = 89/277 (32.13%), Postives = 153/277 (55.23%), Query Frame = 0

Query: 425 TRPVRRLYIENLPHSASEKAIIDYLNDFLISPGVNRTEGTQPCISCILHKDRGQALVEFL 484
           TR  RRLY+ N+P   +E+ ++++ N  +   G+ +  G+ P ++C ++ D+  A +EF 
Sbjct: 89  TRQARRLYVGNIPFGVTEEEMMEFFNQQMHLVGLAQAAGS-PVLACQINLDKNFAFLEFR 148

Query: 485 TPDHASAALSFDGSDFSGSILKIRRPKDYVEIV----TGDLDKSVVVVNEISDVVEDSPN 544
           + D  + A++FDG +  G  LKIRRP DY  +     T  +  +VV    IS VV DSP+
Sbjct: 149 SIDETTQAMAFDGINLKGQSLKIRRPHDYQPMPGITDTPAIKPAVVSSGVISTVVPDSPH 208

Query: 545 KIIVAGISNRISSEMLRDIVTAFGPLKAYHF--EINNELNEPCAFLEYDDQSVMPKACAG 604
           KI + G+ N ++ + +++++ +FG L+A++   +    L++  AF EY D S+  ++ AG
Sbjct: 209 KIFIGGLPNYLNDDQVKELLLSFGKLRAFNLVKDAATGLSKGYAFCEYVDLSITDQSIAG 268

Query: 605 LNGLKIGGGVLKVFPAVPFAPLERNGWQPCYVIPEHVKPLLQ-----QPTAVLKVNNVFN 664
           LNG+++G   L V  A   A   +N       +   V  L        PT VL + N+  
Sbjct: 269 LNGMQLGDKKLIVQRASVGAKNAQNAANTTQSVMLQVPGLSNVVTSGPPTEVLCLLNMVT 328

Query: 665 ADVLSVLSESDIEEVLEDIRFECARFGTVKSMNVAKP 691
            D L    E + E++LEDI+ EC ++G V+S+ + +P
Sbjct: 329 PDELR--DEEEYEDILEDIKEECTKYGVVRSVEIPRP 362

BLAST of Sed0025551 vs. ExPASy Swiss-Prot
Match: P90978 (Splicing factor U2AF 65 kDa subunit OS=Caenorhabditis elegans OX=6239 GN=uaf-1 PE=2 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 1.6e-33
Identity = 84/272 (30.88%), Postives = 155/272 (56.99%), Query Frame = 0

Query: 429 RRLYIENLPHSASEKAIIDYLNDFLISPGVNRTEGTQPCISCILHKDRGQALVEFLTPDH 488
           RRLY+ N+P   +E+A++D+ N  +   G+ +  G  P + C ++ D+  A +EF + D 
Sbjct: 184 RRLYVGNIPFGCNEEAMLDFFNQQMHLCGLAQAPG-NPILLCQINLDKNFAFIEFRSIDE 243

Query: 489 ASAALSFDGSDFSGSILKIRRPKDYVEIV-TGDLDKSVVVVNEISDVVEDSPNKIIVAGI 548
            +A ++FDG +F G  LK+RRP+DY     T D++  +     +S +V DS NKI + G+
Sbjct: 244 TTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFDMNSRM----PVSTIVVDSANKIFIGGL 303

Query: 549 SNRISSEMLRDIVTAFGPLKAYHFEINNELN-EPCAFLEYDDQSVMPKACAGLNGLKIGG 608
            N ++ + +++++ +FGPLKA+   ++++ N +  AF EY D ++  +A AGLNG+++G 
Sbjct: 304 PNYLTEDQVKELLCSFGPLKAFSLNVDSQGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGD 363

Query: 609 GVLKVFPAVPFAPLERNGWQPCYVIPEHVKPL--------LQQPTAVLKVNNVFNADVLS 668
             L V   +  A  +R+       +P     +          + T +L + N+   D L 
Sbjct: 364 KQLVV--QLACANQQRHNTN----LPNSASAIAGIDLSQGAGRATEILCLMNMVTEDELK 423

Query: 669 VLSESDIEEVLEDIRFECARFGTVKSMNVAKP 691
             ++ + EE+LED+R EC+++G V+S+ + +P
Sbjct: 424 --ADDEYEEILEDVRDECSKYGIVRSLEIPRP 442

BLAST of Sed0025551 vs. ExPASy Swiss-Prot
Match: P90727 (Splicing factor U2AF 65 kDa subunit OS=Caenorhabditis briggsae OX=6238 GN=uaf-1 PE=3 SV=2)

HSP 1 Score: 145.2 bits (365), Expect = 3.6e-33
Identity = 87/267 (32.58%), Postives = 151/267 (56.55%), Query Frame = 0

Query: 429 RRLYIENLPHSASEKAIIDYLNDFLISPGVNRTEGTQPCISCILHKDRGQALVEFLTPDH 488
           RRLY+ N+P   +E+A++D+ N  +    + +  G  P + C ++ D+  A +EF + D 
Sbjct: 175 RRLYVGNIPFGCNEEAMLDFFNQQMHLCNLAQAPG-NPILLCQINLDKNFAFIEFRSIDE 234

Query: 489 ASAALSFDGSDFSGSILKIRRPKDYVEIV-TGDLDKSVVVVNEISDVVEDSPNKIIVAGI 548
            +A ++FDG +F G  LK+RRP+DY     T D++  +     +S +V DS NKI + G+
Sbjct: 235 TTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFDMNARM----PVSSIVVDSANKIFIGGL 294

Query: 549 SNRISSEMLRDIVTAFGPLKAYHFEINNELN-EPCAFLEYDDQSVMPKACAGLNGLKIGG 608
            N ++ + +++++ +FGPLKA+   ++++ N +  AF EY D ++  +A AGLNG+++G 
Sbjct: 295 PNYLTEDQVKELLCSFGPLKAFSLNVDSQGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGD 354

Query: 609 GVLKVFPAVPFAPLERNGWQPCYVIPEHVKPLLQ---QPTAVLKVNNVFNADVLSVLSES 668
             L V  A        N   P          L Q   + T +L + N+   D L   S+ 
Sbjct: 355 KQLVVQLACA-NQTRHNTHLPNSASAIAGIDLSQGAGRATEILCLMNMVTEDELR--SDE 414

Query: 669 DIEEVLEDIRFECARFGTVKSMNVAKP 691
           D EE+LED+R EC+++G V+S+ + +P
Sbjct: 415 DYEEILEDVREECSKYGIVRSLEIPRP 433

BLAST of Sed0025551 vs. ExPASy TrEMBL
Match: A0A6J1HJZ8 (splicing factor U2af large subunit A-like OS=Cucurbita moschata OX=3662 GN=LOC111464254 PE=4 SV=1)

HSP 1 Score: 1341.3 bits (3470), Expect = 0.0e+00
Identity = 727/923 (78.76%), Postives = 797/923 (86.35%), Query Frame = 0

Query: 1   MSTYSCSKQHSRSSAKKSLYNSSDESAARTRPFSFEDIMLRRKNKGSGSNVEGVDAGSHS 60
           MSTYSCSKQ+SR+SAK+SLYNS+DESAARTRPFSFEDIMLRRKNK S +N +G D GSHS
Sbjct: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAAN-DGGDTGSHS 60

Query: 61  RRESIDKHVTANRESERHFRHSKGSSLDVQNPSLVESTKVSSRRKKEETLLKDKMVVRSD 120
           RRESIDKH+TAN ESERHFRHS+GSSLD+QN  L ES K+SSRRKKEETLLKD MV+RSD
Sbjct: 61  RRESIDKHITANLESERHFRHSRGSSLDLQNLPLEESAKISSRRKKEETLLKDNMVIRSD 120

Query: 121 RNNHESRLTLMGKQKYDTNGKGKRQKYGQENLGWGKNEHRSRFEIENENGKRHSKDAGGK 180
           RNN+ES LTL+GK K DTNGK KRQKYGQENLGWGKN+ RSR +IENE GKR S+DA GK
Sbjct: 121 RNNYESGLTLIGKLKRDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRRSRDAAGK 180

Query: 181 DRHEDRAKGKIERESKRKYEHGEDDRNRDKHIPKRHDHDKHHDLENRERKETKISSSSHY 240
           DR EDR KGK ERESKRKY+HG+DDRNRD+HI K+HDH KHHDLE++ERKE KIS SSH+
Sbjct: 181 DRREDRGKGKSERESKRKYQHGDDDRNRDRHITKKHDHGKHHDLESKERKEAKISLSSHH 240

Query: 241 EDSKLKRRRKRSQDRESKHKRSGSRSPRPPKHSTKLARQNELPLDSHVNKSGRWISDSDK 300
           EDS+LK+RRKRS D +SKHK+SGS SPRPPK STKLARQ ELPLDSHV KSGRW SDSD+
Sbjct: 241 EDSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDR 300

Query: 301 TRDFTNSSSNQYKRHSGST-SGLGGYSPRKRRTESAVKTPSPVQSLEKKKEVSDLHPLEK 360
             DFTNSSS+QY+RHSGST SGLGGYSPRKRRTESAVKTP PV+S EKK E  DL P E 
Sbjct: 301 IGDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTP-PVRSPEKKNEGLDLLPAET 360

Query: 361 VGLLPGSATSNFQLSNPIISLGISNDQSGGALFSPSMGKPLLEVSSNNLATKTNASLDLI 420
           VGL  GS TSNFQ SNP +S GISND SGGALFS +MGK L  VSSNNLATKTNAS+D I
Sbjct: 361 VGLFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASIDSI 420

Query: 421 QLTQATRPVRRLYIENLPHSASEKAIIDYLNDFLISPGVNRTEGTQPCISCILHKDRGQA 480
           QLTQATRP+RRLYIENLPHS SEKAIID LN FL S GVN  EGTQPCISCI+HKDRGQA
Sbjct: 421 QLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQA 480

Query: 481 LVEFLTPDHASAALSFDGSDFSGSILKIRRPKDYVEIVTGDLDKSVVVVNEISDVVEDSP 540
           LVEFLTP+ ASAALSFDGSDFSGSIL+IRRPKDYVE+VTGDL+KSV VVN+ISDVVEDSP
Sbjct: 481 LVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVTGDLEKSVAVVNKISDVVEDSP 540

Query: 541 NKIIVAGISNRISSEMLRDIVTAFGPLKAYHFEINNELNEPCAFLEYDDQSVMPKACAGL 600
           NKIIVAGISNRISSEML++IV AFGPLKAYHFEINN+LNEPCAFLEY DQSVMPKACAGL
Sbjct: 541 NKIIVAGISNRISSEMLKNIVAAFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGL 600

Query: 601 NGLKIGGGVLKVFPAVPFAPLERNGWQPCYVIPEHVKPLLQQPTAVLKVNNVFNADVLSV 660
           NG+KIGG VLKVFPAVP A LERNG QPCY IPEHVKPLLQQPT VLKV NVFNADVL +
Sbjct: 601 NGMKIGGQVLKVFPAVPIASLERNGCQPCYGIPEHVKPLLQQPTVVLKV-NVFNADVLPL 660

Query: 661 LSESDIEEVLEDIRFECARFGTVKSMNVAKPCRSCVNAEEEYKNNSDATDVVVKHEIQEI 720
           LSESDI EVLEDIRFECARFGTVKSMNVAK C SCV+AEEEYK+  D TDV +KHEIQEI
Sbjct: 661 LSESDINEVLEDIRFECARFGTVKSMNVAKSCTSCVSAEEEYKSICDVTDVEIKHEIQEI 720

Query: 721 SPIVISRNDNGLEDNKAKLDNCPDSTYQEQGDCPMNDRHQNEVVEDKLCQMGSNDATHF- 780
           S IVISRND   +      DNCPD TYQEQG+ P N RH+NEVVEDKLC MG  DAT F 
Sbjct: 721 STIVISRNDTDHD------DNCPDDTYQEQGNYPHNGRHKNEVVEDKLCHMGIKDATCFE 780

Query: 781 NVACEGASETITQGLDDQRSSPGNELHDAKAAKIIETDETGSDVKSVLLGDSTTVMADSE 840
           +V C+ ASETI +G  D++SSPGNELHD K AKII   ETGSD K VLLGDSTTV+AD E
Sbjct: 781 DVVCKSASETIPRGCCDKQSSPGNELHDVKVAKII---ETGSDEKPVLLGDSTTVVADDE 840

Query: 841 KKVLNGLDPIVGTDCGDTSEKSKEKDPNNNLESLYASGSVFVEFGRMEASYMAAHAFHGR 900
           KKVL+G+DP+V TD  DTSEK ++K+ NNNLES +A GSVFVEFGR+EAS MAAH+ HGR
Sbjct: 841 KKVLHGMDPMVRTD-SDTSEKREKKERNNNLESSFAVGSVFVEFGRLEASCMAAHSLHGR 900

Query: 901 IYDGREISVEFFPRDLYHKRFPK 922
           IYDG+EISVE+ P DLYH+RFPK
Sbjct: 901 IYDGQEISVEYIPHDLYHRRFPK 910

BLAST of Sed0025551 vs. ExPASy TrEMBL
Match: A0A6J1KDP6 (splicing factor U2af large subunit A-like OS=Cucurbita maxima OX=3661 GN=LOC111494723 PE=4 SV=1)

HSP 1 Score: 1328.2 bits (3436), Expect = 0.0e+00
Identity = 720/923 (78.01%), Postives = 793/923 (85.92%), Query Frame = 0

Query: 1   MSTYSCSKQHSRSSAKKSLYNSSDESAARTRPFSFEDIMLRRKNKGSGSNVEGVDAGSHS 60
           MSTYSCSKQ+SR+SAK+ LYNS+DESAARTRPFSFEDIMLRRKNK S +  +G D  S S
Sbjct: 1   MSTYSCSKQYSRTSAKQCLYNSNDESAARTRPFSFEDIMLRRKNKESAA-TDGGDTSSQS 60

Query: 61  RRESIDKHVTANRESERHFRHSKGSSLDVQNPSLVESTKVSSRRKKEETLLKDKMVVRSD 120
           RRESIDKH+TANRESERHFRHS+GSSLDVQN  L ES K+SSRRKKEETLLKD MV+RSD
Sbjct: 61  RRESIDKHITANRESERHFRHSRGSSLDVQNLPLEESAKISSRRKKEETLLKDNMVIRSD 120

Query: 121 RNNHESRLTLMGKQKYDTNGKGKRQKYGQENLGWGKNEHRSRFEIENENGKRHSKDAGGK 180
           RNN+ES LTL+GK K+DTNGK KRQKYGQENLGWGKN+ RSR +IENE GKRHS+DA GK
Sbjct: 121 RNNYESGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGK 180

Query: 181 DRHEDRAKGKIERESKRKYEHGEDDRNRDKHIPKRHDHDKHHDLENRERKETKISSSSHY 240
           DR E+R KGK ERESKRKY+HG+DDRNRD+HI K+HDH KHHDLE++ERKE KIS SSH+
Sbjct: 181 DRRENRGKGKSERESKRKYQHGDDDRNRDRHITKKHDHGKHHDLESKERKEAKISLSSHH 240

Query: 241 EDSKLKRRRKRSQDRESKHKRSGSRSPRPPKHSTKLARQNELPLDSHVNKSGRWISDSDK 300
           EDS+LK+RRKRS DR+SKHK+SGS SPRPPK STKLARQ ELPLDSHV KSGRW SDSD+
Sbjct: 241 EDSRLKKRRKRSLDRQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDR 300

Query: 301 TRDFTNSSSNQYKRHSGST-SGLGGYSPRKRRTESAVKTPSPVQSLEKKKEVSDLHPLEK 360
             DFTNSSS+QY+RHSG T SGLGGYSPRKRRTESAVKTP PV+S EKK E  DL P EK
Sbjct: 301 IGDFTNSSSSQYRRHSGLTRSGLGGYSPRKRRTESAVKTP-PVRSPEKKNEGLDLLPAEK 360

Query: 361 VGLLPGSATSNFQLSNPIISLGISNDQSGGALFSPSMGKPLLEVSSNNLATKTNASLDLI 420
           VGL  GS TSNFQ SNP +S GISND SGGALFS + GK L  +SSNNLATKTNAS D I
Sbjct: 361 VGLFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLTTGKSLSGISSNNLATKTNASFDSI 420

Query: 421 QLTQATRPVRRLYIENLPHSASEKAIIDYLNDFLISPGVNRTEGTQPCISCILHKDRGQA 480
           QLTQATRP+RRLYIENLPHS SEKAIID LN FL S GVN  EGTQPCISCI+HKDRGQA
Sbjct: 421 QLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQA 480

Query: 481 LVEFLTPDHASAALSFDGSDFSGSILKIRRPKDYVEIVTGDLDKSVVVVNEISDVVEDSP 540
           LVEFLTP+ ASAALSFDGSDFSGSIL+IRRPKDYVE+VTGDLDKSV VVN+I+DVVEDSP
Sbjct: 481 LVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVTGDLDKSVAVVNKINDVVEDSP 540

Query: 541 NKIIVAGISNRISSEMLRDIVTAFGPLKAYHFEINNELNEPCAFLEYDDQSVMPKACAGL 600
           NKII+AGISNRISSEML++IV AFGPLKAYHFEINN+LNEPCAFLEY DQSVMPKACAGL
Sbjct: 541 NKIIIAGISNRISSEMLKNIVAAFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGL 600

Query: 601 NGLKIGGGVLKVFPAVPFAPLERNGWQPCYVIPEHVKPLLQQPTAVLKVNNVFNADVLSV 660
           NG+KIGG VLKVFPAVP A LERNG QPCY IPEHVKPLLQQPT VLKV NVFNADVL +
Sbjct: 601 NGMKIGGEVLKVFPAVPIASLERNGCQPCYGIPEHVKPLLQQPTVVLKV-NVFNADVLPL 660

Query: 661 LSESDIEEVLEDIRFECARFGTVKSMNVAKPCRSCVNAEEEYKNNSDATDVVVKHEIQEI 720
           LSESDI EVLEDIRFECARFGTVKSMNVAKPC SCV+AEEEY++  D TDV +KHEIQEI
Sbjct: 661 LSESDINEVLEDIRFECARFGTVKSMNVAKPCTSCVSAEEEYQSICDITDVEIKHEIQEI 720

Query: 721 SPIVISRNDNGLEDNKAKLDNCPDSTYQEQGDCPMNDRHQNEVVEDKLCQMGSNDATHF- 780
           S IVISRND   +      DNC D TYQEQG+ P N RH+NEVVEDKLC MG  DAT F 
Sbjct: 721 STIVISRNDTDHD------DNCLDYTYQEQGNYPHNGRHKNEVVEDKLCHMGIKDATCFE 780

Query: 781 NVACEGASETITQGLDDQRSSPGNELHDAKAAKIIETDETGSDVKSVLLGDSTTVMADSE 840
           +V C+ A ETI +G  D++SSPGNELH  K AKII   ETGSD K VLLGDS+TV+AD E
Sbjct: 781 DVVCKSALETIPRGCCDKQSSPGNELHGVKVAKII---ETGSDEKPVLLGDSSTVVADDE 840

Query: 841 KKVLNGLDPIVGTDCGDTSEKSKEKDPNNNLESLYASGSVFVEFGRMEASYMAAHAFHGR 900
           KKVL+G+DP+V TD  DTSEK ++K+ NNNLES +A GSVFVEFGR+EAS MAAH+ HGR
Sbjct: 841 KKVLHGMDPMVRTD-SDTSEKREKKERNNNLESSFAVGSVFVEFGRLEASCMAAHSLHGR 900

Query: 901 IYDGREISVEFFPRDLYHKRFPK 922
           IYDG+EISVE+ P DLYHKRFPK
Sbjct: 901 IYDGQEISVEYIPHDLYHKRFPK 910

BLAST of Sed0025551 vs. ExPASy TrEMBL
Match: A0A6J1C9Y6 (splicing factor U2af large subunit A OS=Momordica charantia OX=3673 GN=LOC111009561 PE=4 SV=1)

HSP 1 Score: 1235.3 bits (3195), Expect = 0.0e+00
Identity = 677/925 (73.19%), Postives = 763/925 (82.49%), Query Frame = 0

Query: 1   MSTYSCSKQHSRSSAKKSLYNSSDESAARTRPFSFEDIMLRRKNKGSGSNVEG--VDAGS 60
           MST+SCSK++SRSS K +LYN++DESAARTRPFSFEDIMLRRKNKGS +NVEG    AGS
Sbjct: 1   MSTHSCSKKYSRSSVKHTLYNTNDESAARTRPFSFEDIMLRRKNKGSAANVEGGATVAGS 60

Query: 61  HSRRESIDKHVTANRESERHFRHSKGSSLDVQNPSLVESTKVSSRRKKEETLLKDKMVVR 120
            SRR+SID  +T NRESER FRHS+GSSLDVQN  L ES K+SSRRKKEETLLKD  VVR
Sbjct: 61  RSRRDSIDNDITDNRESERLFRHSRGSSLDVQNLPLEESAKISSRRKKEETLLKDSTVVR 120

Query: 121 SDRNNHESRLTLMGKQKYDTNGKGKRQKYGQENLGWGKNEHRSRFEIENENGKRHSKDAG 180
           SDRNN+ES LTLMGK KY+TNGK K QKYGQENLGWGK++ R+R +IENE+GKRHS+D  
Sbjct: 121 SDRNNYESGLTLMGKVKYETNGKDKGQKYGQENLGWGKSDQRTRIDIENESGKRHSRDLA 180

Query: 181 GKDRHEDRAKGKIERESKRKYEHGEDDRNRDKHIPKRHDHDKHHDLENRERKETKISSSS 240
           GKDR EDR +GK ERESKRKY++G+DD+NRD++  K+HDH KH DLENR+RKE K+S SS
Sbjct: 181 GKDRREDRGRGKFERESKRKYQNGDDDKNRDRYSEKKHDHSKHRDLENRDRKEAKVSLSS 240

Query: 241 HYEDSKLKRRRKRSQDRESKHKRSGSRSPRPPKHSTKLARQNELPLDSHVNKSGRWISDS 300
           HYEDS+ KRRRKRSQDRESKH+RS S SPR  KHSTKL R  ELP DSH+ KSGRW SDS
Sbjct: 241 HYEDSRFKRRRKRSQDRESKHRRSVSLSPRAHKHSTKLVRHKELP-DSHLKKSGRWRSDS 300

Query: 301 DKTRDFTNSSSNQYKRHSGSTSGLGGYSPRKRRTESAVKTPSPVQSLEKKKEVSDLHPLE 360
           D+T DFTNSS++QY+RHSGSTSGLGGYSPRKRRTESA+KTPSPV+S EKK E  DL P +
Sbjct: 301 DRTGDFTNSSTSQYRRHSGSTSGLGGYSPRKRRTESAIKTPSPVRSPEKKNEALDLLPAD 360

Query: 361 KVGLLPGSATSNFQLSNPIISLGISNDQSGGALFSPSMGKPLLEVSSNNLATKT-NASLD 420
           KVGL  GS TS FQ SNP +S  IS DQS GALFS +MGK L  VSSNNL  KT N SLD
Sbjct: 361 KVGLFSGSVTSIFQPSNPTVSSVISTDQSSGALFSSAMGKSLSGVSSNNLPMKTANVSLD 420

Query: 421 LIQLTQATRPVRRLYIENLPHSASEKAIIDYLNDFLISPGVNRTEGTQPCISCILHKDRG 480
           LIQLTQATRP+RRLYIEN+PHSASEKA+I+ +N FL S GVN  EGTQPCISCI+HKDRG
Sbjct: 421 LIQLTQATRPMRRLYIENVPHSASEKAVIECMNGFLTSSGVNHIEGTQPCISCIIHKDRG 480

Query: 481 QALVEFLTPDHASAALSFDGSDFSGSILKIRRPKDYVEIVTGDLDKSVVVVNEISDVVED 540
           QALVEFLTP+ ASAALSFDG++FSGSILKIRRPKDYVEIVTGDLDKS  VVNEISDVVED
Sbjct: 481 QALVEFLTPEDASAALSFDGTNFSGSILKIRRPKDYVEIVTGDLDKSEAVVNEISDVVED 540

Query: 541 SPNKIIVAGISNRISSEMLRDIVTAFGPLKAYHFEINNELNEPCAFLEYDDQSVMPKACA 600
           SPNKI +AGISNRISSEMLRDIVT+FGPLKAYHFEIN +LNEPCAFLEY DQSVMPKACA
Sbjct: 541 SPNKIFIAGISNRISSEMLRDIVTSFGPLKAYHFEINGDLNEPCAFLEYVDQSVMPKACA 600

Query: 601 GLNGLKIGGGVLKVFPAVPFAPLERNGWQPCYVIPEHVKPLLQQPTAVLKVNNVFNADVL 660
           GLNG+KIGG VLKVF AVPFAPLERNG QPCY IPEHVKPLLQQPT VLK+NNVF ADVL
Sbjct: 601 GLNGMKIGGEVLKVFSAVPFAPLERNGCQPCYGIPEHVKPLLQQPTVVLKLNNVFGADVL 660

Query: 661 SVLSESDIEEVLEDIRFECARFGTVKSMNVAKPCRSCVNAEEEYKNNSDATDVVVKHEIQ 720
            VLSES+I+EVLEDIRFECARFGTVKSMNV KPC S V AEE+YKN +   DV +KH IQ
Sbjct: 661 PVLSESNIDEVLEDIRFECARFGTVKSMNVVKPCNSFVGAEEKYKNTN---DVEIKHGIQ 720

Query: 721 EISPIVISRNDNGLEDNKAKLDNCPDSTYQEQGDCPMNDRHQNEVVEDKLCQMGSNDATH 780
             S +V SRNDN LED++A L++ P                 NEVVE+KLCQMG++DA  
Sbjct: 721 GNSTLVPSRNDNDLEDDEANLNDRP-----------------NEVVENKLCQMGNDDAPC 780

Query: 781 F-NVACEGASETITQGLDDQRSSPGNELHDAKAAKIIETDETGSDVKSVLLGDSTTVMAD 840
           F +VACE   E+I  G+  ++SSPGNEL D   A+++ETDE GSD KS+LL DSTTV AD
Sbjct: 781 FEDVACENTLESIPGGIPGRQSSPGNELRDTNVAEMVETDEKGSDNKSMLLDDSTTVGAD 840

Query: 841 SEKKVLNGLDPIVGTDCGDTSEKSKEKDPNNNLESLYASGSVFVEFGRMEASYMAAHAFH 900
           S+KKVLNGLD +V TD  D  EK ++KDP  N   L+  GSVFVEFGRMEAS MAAH+ H
Sbjct: 841 SKKKVLNGLDAMVRTD-SDAFEKVEKKDPRTN---LFVLGSVFVEFGRMEASCMAAHSLH 900

Query: 901 GRIYDGREISVEFFPRDLYHKRFPK 922
           GRIYDG+EI +E+ P DLY KRFPK
Sbjct: 901 GRIYDGQEICIEYIPHDLYRKRFPK 900

BLAST of Sed0025551 vs. ExPASy TrEMBL
Match: A0A6J1H6S0 (splicing factor U2af large subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC111460135 PE=4 SV=1)

HSP 1 Score: 1185.2 bits (3065), Expect = 0.0e+00
Identity = 653/922 (70.82%), Postives = 729/922 (79.07%), Query Frame = 0

Query: 1   MSTYSCSKQHSRSSAKKSLYNSSDESAARTRPFSFEDIMLRRKNKGSGSNVEGVDAGSHS 60
           MS+YS SK +SR S K++L NS+DESAARTRP SFEDIMLRRKNKGS   VE    GSHS
Sbjct: 1   MSSYSFSKHYSRRSKKQTLSNSNDESAARTRPLSFEDIMLRRKNKGSADTVEVGVTGSHS 60

Query: 61  RRESIDKHVTANRESERHFRHSKGSSLDVQNPSLVESTKVSSRRKKEETLLKDKMVVRSD 120
           RRESIDK  T N  SERHFRH KG+SLDVQN SL ES K SSRRKKEETLLKD M VRSD
Sbjct: 61  RRESIDKLSTDNCASERHFRHGKGASLDVQNLSLEESGKDSSRRKKEETLLKDNMEVRSD 120

Query: 121 RNNHESRLTLMGKQKYDTNGKGKRQKYGQENLGWGKNEHRSRFEIENENGKRHSKDAGGK 180
           RNN++S LTLMGK KYD NG  K+QKYGQEN+G GK    SR +IE E GKRHS+D+  K
Sbjct: 121 RNNYDSELTLMGKLKYDANGNDKKQKYGQENVGRGKKNQSSRVDIEKETGKRHSRDS--K 180

Query: 181 DRHEDRAKGKIERESKRKYEHGEDDRNRDKHIPKRHDHDKHHDLENRERKETKISSSSHY 240
            + ED  +G  ER  KRK ++G+D+ NRDK+  KRHDH KHHD ENRERKE K S +S+Y
Sbjct: 181 AKREDLGRGNFERGGKRKDQNGDDEGNRDKYAAKRHDHGKHHDPENRERKEAKASLTSNY 240

Query: 241 EDSKLKRRRKRSQDRESKHKRSGSRSPRPPKHSTKLARQNELPLDSHVNKSGRWISDSDK 300
           EDS+L+RRRKRSQDRESKH+RS S SPRP KHS+KL RQ ELPLDSHV KSGRW SDSD+
Sbjct: 241 EDSRLQRRRKRSQDRESKHRRSVSLSPRPHKHSSKLVRQKELPLDSHVKKSGRWRSDSDR 300

Query: 301 TRDFTNSSSNQYKRHSGSTSGLGGYSPRKRRTESAVKTPSPVQSLEKKKEVSDLHPLEKV 360
           T D TNSS++QY+RH GSTSGLGGYSPRKRRTESA+KTPSPVQS EKK E  D+ P EK+
Sbjct: 301 TGDLTNSSNSQYRRHFGSTSGLGGYSPRKRRTESAIKTPSPVQSPEKKDEGLDVPPTEKI 360

Query: 361 GLLPGSATSNFQLSNPIISLGISNDQSGGALFSPSMGKPLLEVSSNNLATKTNASLDLIQ 420
           GL   S  SNFQ SN  +S GI N QSGGA+FS  +GK L  VSSNN+  KTN SLDLIQ
Sbjct: 361 GLFSSSINSNFQPSNATVSSGIINVQSGGAIFSSVIGKSLTGVSSNNIEMKTNVSLDLIQ 420

Query: 421 LTQATRPVRRLYIENLPHSASEKAIIDYLNDFLISPGVNRTEGTQPCISCILHKDRGQAL 480
           LTQATRP+RRLYIENLPHSASEKAIID LN FL S GVN  +GT PCISCI+HKDRGQAL
Sbjct: 421 LTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIKGTLPCISCIIHKDRGQAL 480

Query: 481 VEFLTPDHASAALSFDGSDFSGSILKIRRPKDYVEIVTGDLDKSVVVVNEISDVVEDSPN 540
           VEFLTP+ ASAALSFDGSDFSGSILKIRRPKDYVEIVTG LDKSV VVN+I D VEDSPN
Sbjct: 481 VEFLTPEDASAALSFDGSDFSGSILKIRRPKDYVEIVTGGLDKSVAVVNKIIDTVEDSPN 540

Query: 541 KIIVAGISNRISSEMLRDIVTAFGPLKAYHFEINNELNEPCAFLEYDDQSVMPKACAGLN 600
           KI +AGISNRISSEMLRDIVTAFGPLKA+HFE+N++LNE CAFLEY DQS++ KACAGLN
Sbjct: 541 KIFIAGISNRISSEMLRDIVTAFGPLKAFHFEMNDDLNERCAFLEYVDQSIVSKACAGLN 600

Query: 601 GLKIGGGVLKVFPAVPFAPLERNGWQPCYVIPEHVKPLLQQPTAVLKVNNVFNADVLSVL 660
           G+KIGG VLKVFPAVPF  LERN  QP Y IPEHVKPLLQQPT VLKVNNVFNADVL VL
Sbjct: 601 GMKIGGEVLKVFPAVPFTSLERNECQPWYGIPEHVKPLLQQPTVVLKVNNVFNADVLPVL 660

Query: 661 SESDIEEVLEDIRFECARFGTVKSMNVAKPCRSCVNAEEEYKNNSDATDVVVKHEIQEIS 720
           SE+DI+EVLEDIR ECARFGTVKSMN  KPC  C +AEE+YKN SD TDV +KHEIQE  
Sbjct: 661 SEADIDEVLEDIRVECARFGTVKSMNFVKPCNGCFSAEEDYKNISDITDVEIKHEIQENC 720

Query: 721 PIVISRNDNGLEDNKAKLDNCPDSTYQEQGDCPMNDRHQNEVVEDKLCQMGSNDATHFN- 780
            + ISRN N +EDN   LDNCP+ T Q QG+CP N RHQ+E VE KLC+MG +DAT F  
Sbjct: 721 TMAISRNGNDVEDNNVNLDNCPNDTNQRQGNCPGNGRHQDE-VEVKLCRMGQDDATRFEI 780

Query: 781 VACEGASETITQGLDDQRSSPGNELHDAKAAKIIETDETGSDVKSVLLGDSTTVMADSEK 840
           VACE ASE I +GL +Q+SSPGN+  DAK A+ IET+E   D KSV + DS  V  D   
Sbjct: 781 VACENASERIPRGLSEQQSSPGNQHQDAKVAETIETNEISPDKKSVCIDDSAMVRTDF-- 840

Query: 841 KVLNGLDPIVGTDCGDTSEKSKEKDPNNNLESLYASGSVFVEFGRMEASYMAAHAFHGRI 900
                          DTSEKS++ DP NNL SL+  GSVFVEFGR EAS MAAH+ HGRI
Sbjct: 841 ---------------DTSEKSEKDDPRNNLASLFVLGSVFVEFGRTEASCMAAHSLHGRI 900

Query: 901 YDGREISVEFFPRDLYHKRFPK 922
           YDG+EIS+E+ P DLY KRFPK
Sbjct: 901 YDGQEISIEYIPHDLYRKRFPK 902

BLAST of Sed0025551 vs. ExPASy TrEMBL
Match: A0A1S3CCY4 (splicing factor U2af large subunit B isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499435 PE=4 SV=1)

HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 661/1013 (65.25%), Postives = 750/1013 (74.04%), Query Frame = 0

Query: 1    MSTYSCSKQHSRSSAKKSLYNSSDESAARTRPFSFEDIMLRRKNKGSGSNVEGVDAGSHS 60
            MST+SCSKQ+SR S K++  NS DE+AARTRPFSFEDIMLRRK KGS + VE        
Sbjct: 1    MSTHSCSKQYSRRSKKQTRSNSDDETAARTRPFSFEDIMLRRKTKGSSATVE-------- 60

Query: 61   RRESIDKHVTANRESERHFRHSKGSSLDVQNPSL-VESTKVSSRRKKEETLLKDKMVVRS 120
                +D   T NR SERHFRHSKGSSLDVQN SL  ES K SSRRKKEET+LK+ +VVR+
Sbjct: 61   ----VDVTSTDNRASERHFRHSKGSSLDVQNVSLEEESAKDSSRRKKEETVLKNNIVVRN 120

Query: 121  DRNNHESRLTLMGKQKYDTNGKGKRQKYGQENLGWGKNEHRSRFEIENENGKRHSKDAGG 180
            DRNN+ES L+LM K K+D N K +R+KYGQENLGWGKN+   R +IE E  KRHS+D   
Sbjct: 121  DRNNYESGLSLMSKLKHDRNEKDEREKYGQENLGWGKNDQSCRIDIETETAKRHSRDTAF 180

Query: 181  KDRHEDRAKGKIERE--------------------------------------------- 240
            KDR +D  +G+ ERE                                             
Sbjct: 181  KDRRQDHGRGEFERESKRKSQNGDDDRNRDSKRKSQNGDDDRNRDSKRKSQNGDDDRSRD 240

Query: 241  ---------------------------------------------SKRKYEHGEDDRNRD 300
                                                         SKRK ++G+DDRNRD
Sbjct: 241  SKRKSQNGDDDRNRDSKRKSQNGDDERNRDGKRKSQNGDDDRNRDSKRKSQNGDDDRNRD 300

Query: 301  KHIPKRHDHDKHHDLENRERKETKISSSSHYEDSKLKRRRKRSQDRESKHKRSGSRSPRP 360
            K+I KRHDH KHHDLENRE+KE  +S +S Y+DS+LKR RKRS DRESKH+RS S SPR 
Sbjct: 301  KYIAKRHDHGKHHDLENREKKEAIVSLTSRYQDSRLKRTRKRSSDRESKHRRSVSLSPRS 360

Query: 361  PKHSTKLARQNELPLDSHVNKSGRWISDSDKTRDFTNSSSNQYKRHSGSTSGLGGYSPRK 420
             KHSTKLARQ ELPLDSHV KSGRW SDS++T DFTN+S++QY+RHSGSTSGLGGYSPRK
Sbjct: 361  HKHSTKLARQKELPLDSHVKKSGRWRSDSERTGDFTNTSNSQYRRHSGSTSGLGGYSPRK 420

Query: 421  RRTESAVKTPSPVQSLEKKKEVSDLHPLEKVGLLPGSATSNFQLSNPIISLGISNDQSGG 480
            RRTESAVKTPSP+ S EKK EV DL P EKVGL  GS  SNF  SNP +SLGISNDQSGG
Sbjct: 421  RRTESAVKTPSPLLSPEKKNEVLDLPPTEKVGLFSGSVASNFSPSNPTVSLGISNDQSGG 480

Query: 481  ALFSPSMGKPLLEVSSNNLATKTNASLDLIQLTQATRPVRRLYIENLPHSASEKAIIDYL 540
            A FS +MGK L  VSSNN+A KT  S DL+QLTQATRP+RRLYIENLPHSASEKAIID L
Sbjct: 481  AFFSSAMGKSLSVVSSNNIAMKTKVSFDLVQLTQATRPMRRLYIENLPHSASEKAIIDCL 540

Query: 541  NDFLISPGVNRTEGTQPCISCILHKDRGQALVEFLTPDHASAALSFDGSDFSGSILKIRR 600
            N FL+S GVN  EGTQPCISCI+HKDRGQALVEFLTP+ ASAALSFDGSDFSGSILKIRR
Sbjct: 541  NGFLMSSGVNHIEGTQPCISCIIHKDRGQALVEFLTPEDASAALSFDGSDFSGSILKIRR 600

Query: 601  PKDYVEIVTGDLDKSVVVVNEISDVVEDSPNKIIVAGISNRISSEMLRDIVTAFGPLKAY 660
            PKDY+E VTGDLDKS+ VVN+ISDVVEDSPNKIIVAGISNR+SSEMLRDIVTAFG LKAY
Sbjct: 601  PKDYIETVTGDLDKSMPVVNKISDVVEDSPNKIIVAGISNRLSSEMLRDIVTAFGRLKAY 660

Query: 661  HFEINNELNEPCAFLEYDDQSVMPKACAGLNGLKIGGGVLKVFPAVPFAPLERNGWQPCY 720
            HFE+N++LN+PCAFLEY D+SV+ KACAGLNG+KIGG VLKVFPAVPF   ER G QPCY
Sbjct: 661  HFEMNDDLNQPCAFLEYIDESVVSKACAGLNGMKIGGQVLKVFPAVPFPSTERTGCQPCY 720

Query: 721  VIPEHVKPLLQQPTAVLKVNNVFNADVLSVLSESDIEEVLEDIRFECARFGTVKSMNVAK 780
             IPEHVKPLLQQP+ VLK+NNVFNADVL VLSESDI+EVLEDIRFECARFGTVKSMN  K
Sbjct: 721  GIPEHVKPLLQQPSVVLKINNVFNADVLPVLSESDIDEVLEDIRFECARFGTVKSMNFVK 780

Query: 781  PCRSCVNAEEEYKNNSDATDVVVKHEIQEISPIVISRNDNGLEDNKAKLDNCPDSTYQEQ 840
            PC   VN EEE+K  SD +DV +KHEIQE S  VI RN N LEDN A LD+CP  T Q+Q
Sbjct: 781  PCNGGVNTEEEHKKISDISDVEIKHEIQENSKTVILRNSNDLEDNNANLDSCPSDTNQKQ 840

Query: 841  GDCPMNDRHQNEVVEDKLCQMGSNDATHFNV-ACEGASETITQGLDDQRSSPGNELHDAK 900
             +C  N RHQ+E VEDKLCQMG+ DAT F V ACE ASE I Q L +QRSSP N+  +AK
Sbjct: 841  ANCSGNGRHQDEAVEDKLCQMGNTDATCFEVAACENASERIRQVLSEQRSSPENDFQNAK 900

Query: 901  AAKIIETDETGSDVKSVLLGDSTTVMADSEKKVLNGLDPIVGTDCGDTSEKSKEKDPNNN 922
              +IIETDETG D K V +  S+ ++AD+EKK LNGLDP+V     +  EKS++KDP+NN
Sbjct: 901  VTEIIETDETGLDKKLVCVEASSMMVADNEKKSLNGLDPVVRI-ASNAVEKSEKKDPDNN 960

BLAST of Sed0025551 vs. TAIR 10
Match: AT2G33440.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 198.4 bits (503), Expect = 2.6e-50
Identity = 133/368 (36.14%), Postives = 196/368 (53.26%), Query Frame = 0

Query: 555 MLRDIVTAFGPLKAYHFEINNELNEPCAFLEYDDQSVMPKACAGLNGLKIGGGVLKVFPA 614
           ML +IV+ FGPLKAY F  NN+LN+ CAFLEY D SV  KACAGLNG+++GG V+    A
Sbjct: 1   MLMEIVSVFGPLKAYRFVSNNDLNQRCAFLEYTDGSVTLKACAGLNGMRLGGSVITAVCA 60

Query: 615 VPFA-PLERNGWQPCYVIPEHVKPLLQQPTAVLKVNNVFNADVLSVLSESDIEEVLEDIR 674
            P A  +  N   P Y IP H KPLL +P  +LK+ NV + + L+  SE +++E+LED+R
Sbjct: 61  FPDASSVAVNENPPFYGIPSHAKPLLGKPKNILKLKNVVDPEDLTSFSEQEVKEILEDVR 120

Query: 675 FECARFGTVKSMNVAK-PCRSCVNAEEEYKNNSDATDVVVKHEIQEISPIVISRNDNGLE 734
            ECARFG +KS+N+ +   +    +E     N ++TD       +E++  VI   D G E
Sbjct: 121 LECARFGVIKSINILEHKSKDITVSETNPLLNLESTD------SKEMNVSVIEEKDEGSE 180

Query: 735 DNKAKLDNCPDSTYQEQGDCPMNDRHQNEVVEDKLCQMGSNDATHFNVACEGASETITQG 794
             +   DN       +  +  M D   +   EDKLC+  S          + A+ET TQ 
Sbjct: 181 KAEDIADNV------DLAEVVMPD---SLTGEDKLCEPCS----------DTAAETSTQE 240

Query: 795 LDDQRSSPGNELHDAKAAKIIETDETGSDVKSVLLGDSTTVMADSEKKVLNGLDPIVGTD 854
            +D  S+           KI+E              +S    A++ ++V +        D
Sbjct: 241 NEDLHST-----EQEHCEKIVE--------------ESAQAEAENPQEVASVRTVKTRWD 300

Query: 855 CGDTSEKSKEKDPNNNLESLYASGSVFVEFGRMEASYMAAHAFHGRIYDGREISVEFFPR 914
            GD  E+ +E+DP    E ++ +G +F+E+ R EA+  AAH+ HGR+YD R +  E+  +
Sbjct: 301 AGDKIEEEQEEDP----EDVFETGCIFIEYRRPEATCDAAHSLHGRLYDNRIVKAEYVSK 320

Query: 915 DLYHKRFP 921
           +LY  RFP
Sbjct: 361 ELYQIRFP 320

BLAST of Sed0025551 vs. TAIR 10
Match: AT2G33435.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 176.4 bits (446), Expect = 1.1e-43
Identity = 163/483 (33.75%), Postives = 243/483 (50.31%), Query Frame = 0

Query: 40  LRRKNKGSGSNVEGVDAGSHSRRESIDKHVTANRESERHFRHSKGSSLDVQNPSLVESTK 99
           L R+ +   S+ +  +A S SR E++DK       S R  R ++G     +  S+ E +K
Sbjct: 512 LSREERVKDSSRKKEEAISSSREENLDKQKKDESTSNRK-RKAEGECSTAETESIEEHSK 571

Query: 100 VSSRRKKEETLLKDKMVVRSDRNNHESRLTLMGKQKYDTNGKGKRQKYGQENLGWGKNEH 159
              R KKEET         +     E R   M K+   +N K + +            E 
Sbjct: 572 -DRRGKKEET---------NSNCREERRDKKMKKEDLASNRKIEGE--------IPTTET 631

Query: 160 RSRFEIENENGKRHSKDAGGKDRHEDRAKGKIERESKRKYEHGEDDRNRDKHIPKRHDHD 219
           ++  + +    K+  +     D   D +  K +   KRK ++G   +NR+ ++ KRHD  
Sbjct: 632 KTMTDRDGLCSKKRLRSLVVADVPRDESSIKPDNGDKRKNQNGNHKKNREINMSKRHDPG 691

Query: 220 KHHDLENRERKETKISSSSHYEDSKLKRRRKRSQDRESKHKRSGSRSPRPPKHSTKLARQ 279
           K H +E  ER E +    S   D + KRRR RS+D     ++  S  PR  K +++  R 
Sbjct: 692 KVHSVEVSERWERREQPKSRQRDLREKRRRSRSRDHGQDRQKRSSPLPRAEKATSRHKRN 751

Query: 280 NELPLDSHVNKSGRWISDSDKTRDFTNSSSNQYKRHSGSTSGLGGYSPRKRRTESAVKTP 339
           +E   ++ V         +D     T++ SN+ +R+S S S LGGYSPRKRR +++ K  
Sbjct: 752 HEERSENVVKDRSGKHHCNDNEDKVTSTVSNKSRRYSASKSELGGYSPRKRREQASTKAA 811

Query: 340 SPVQ-SLEKKKEVSDLHPLEKVGLLPGSATSNFQLSNPIISLGISNDQSGGALFSPSMGK 399
           SP   S EKK     L      G+   S  S  Q +       IS  ++   L  P M  
Sbjct: 812 SPPNLSSEKKSAKWGLAATVTAGMFSDSVFSGLQAATQTAYPTIS--EASLTLLKPLM-- 871

Query: 400 PLLEVSSNNLATKTNASLDLIQLTQATRPVRRLYIENLPHSASEKAIIDYLNDFLISPGV 459
            +++        +   S D +QLT++TR +RRLY EN+P SASEK++I+  N +++S G 
Sbjct: 872 -VMDAPFRTPPARQTTSFDSVQLTESTRRMRRLYAENVPDSASEKSLIECFNGYMLSSGS 931

Query: 460 NRTEGTQPCISCILHKDRGQALVEFLTPDHASAALSFDGSDFSGSILKIRRPKDYVEIVT 519
           N  +G++PCISCI++K++ QALVEFLTP  ASAALS DG  F+GS LKIRRPKDYV    
Sbjct: 932 NHIKGSEPCISCIINKEKSQALVEFLTPQDASAALSLDGCSFAGSNLKIRRPKDYVRTTV 970

Query: 520 GDL 522
             +
Sbjct: 992 SSI 970

BLAST of Sed0025551 vs. TAIR 10
Match: AT1G60900.1 (U2 snRNP auxilliary factor, large subunit, splicing factor )

HSP 1 Score: 102.8 bits (255), Expect = 1.5e-21
Identity = 141/551 (25.59%), Postives = 239/551 (43.38%), Query Frame = 0

Query: 164 EIENENGKRHSKDAGGKDRHEDRAKGKIERESKRKYEHGEDDRNRDKHIPKRHDHDKHHD 223
           E +NENG+   KD G KDR  DR K +   +S+ +      DR+RD+   K    D+HH 
Sbjct: 51  EKDNENGR--DKD-GNKDR--DREKDRDREKSRDRDREKSRDRDRDRERSKDRQRDRHHR 110

Query: 224 LENRER------KETKISSSSHYEDSKLKRRRKRSQDRESKHKRSGSRSPRPPKHSTKLA 283
             +R+R      K   +    H       RRR R +DRE +H+R  SRS R    S + +
Sbjct: 111 DRHRDRSRERSEKRDDLDDDHHRRSRDRDRRRSRDRDREVRHRRR-SRS-RSRSRSERRS 170

Query: 284 RQNELPLDSHVNKSGRWISDSDKTRDFTNSSSNQYKRHSGSTSGLGGYSPRKRRTESAVK 343
           R        H ++S              + S ++ KR SG        +P      +AV 
Sbjct: 171 RSEHRHKSEHRSRS-------------RSRSRSKSKRRSGF-----DMAPPDMLAATAVA 230

Query: 344 TPSPVQSLEKKKEVSDLHPLEKVGLLPGSATSNFQLSNPIISLGISNDQSGGALFSPSMG 403
               V S            +     +PG  ++ F    P++       Q  GAL      
Sbjct: 231 AAGQVPS------------VPTTATIPGMFSNMF----PMVP-----GQQLGAL------ 290

Query: 404 KPLLEVSSNNLATKTNASLDLIQLTQATRPVRRLYIENLPHSASEKAIIDYLNDFLISPG 463
            P+L V +                 QATR  RR+Y+  LP +A+E+++  + +  + + G
Sbjct: 291 -PVLPVQA--------------MTQQATRHARRVYVGGLPPTANEQSVSTFFSQVMSAIG 350

Query: 464 VNRTEGTQPCISCILHKDRGQALVEFLTPDHASAALSFDGSDFSGSILKIRRPKDYVEIV 523
            N        ++  ++ ++  A VE  + + AS A++ DG    G  +K+RRP DY   +
Sbjct: 351 GNTAGPGDAVVNVYINHEKKFAFVEMRSVEEASNAMALDGIILEGVPVKVRRPTDYNPSL 410

Query: 524 TGDLDKS---------VVVVNEISDVVEDSPNKIIVAGISNRISSEMLRDIVTAFGPLKA 583
              L  S          V ++  S    + P++I V G+    +   +R+++ +FGPL+ 
Sbjct: 411 AATLGPSQPNPNLNLGAVGLSSGSTGGLEGPDRIFVGGLPYYFTEVQIRELLESFGPLRG 470

Query: 584 YHFEINNEL--NEPCAFLEYDDQSVMPKACAGLNGLKIGGGVLKVFPAVPFAPLERNGWQ 643
           ++   + E   ++  AF  Y D SV   ACA LNG+K+G   L V  A+  A   +   +
Sbjct: 471 FNLVKDRETGNSKGYAFCVYQDPSVTDIACAALNGIKMGDKTLTVRRAIQGAIQPKPEQE 530

Query: 644 PCYVIPEH---VKPLLQQ----PTAVLKVNNVFNADVLSVLSESDIEEVLEDIRFECARF 691
              +  +    ++ L+ Q    PT ++ +  V  AD L    + +  E++ED+R E  +F
Sbjct: 531 EVLLYAQQQIALQRLMFQPGGTPTKIVCLTQVVTADDLR--DDEEYAEIMEDMRQEGGKF 532

BLAST of Sed0025551 vs. TAIR 10
Match: AT4G36690.1 (U2 snRNP auxilliary factor, large subunit, splicing factor )

HSP 1 Score: 94.0 bits (232), Expect = 6.9e-19
Identity = 141/556 (25.36%), Postives = 242/556 (43.53%), Query Frame = 0

Query: 161 SRFEIENENG----KRHSKDAGGKDRH-EDRAKGKIERESKRKYEHGEDDRNRDKHIPKR 220
           S FE    NG      + ++ GG+D   ED+   K +RES R +E  E  R++D+   K 
Sbjct: 2   SEFEDHEGNGTVADAIYDEENGGRDGEIEDQLDSKPKRES-RDHER-ETSRSKDREREKG 61

Query: 221 HDHDKHHDLENRERKETKISSSSHYEDSKLKRRRKRSQDRESKHKRSGSRSPRPPKHSTK 280
            D D+  D E        +S  S   D +  + R R +DR+  H+    RS R   HS +
Sbjct: 62  RDKDRERDSE--------VSRRSRDRDGEKSKERSRDKDRD--HRERHHRSSRHRDHSRE 121

Query: 281 LARQNELPLDSHVNKSGRWISDSDKTRDFTNSSSNQYKRHSGSTSGLGGYSPRKRRTESA 340
              + E        + GR   D D  R   +   ++ +   G        S  K R+E  
Sbjct: 122 RGERRE--------RGGR---DDDDYRRSRDRDHDRRRDDRGGRRSRRSRSRSKDRSERR 181

Query: 341 VKTPSPVQSLEKKKEVS--DLHPLEKVGLLPGSA-TSNFQLSNPIISLGISNDQSGGALF 400
            ++ SP +S   K+ VS  D+ P     L  G+A T     + P +          GA  
Sbjct: 182 TRSRSPSKS---KQRVSGFDMAPPASAMLAAGAAVTGQVPPAPPTLP---------GAGM 241

Query: 401 SPSMGKPLLEVSSNNLATKTNASLDLIQLTQATRPVRRLYIENLPHSASEKAIIDYLNDF 460
            P+M       S   L+     ++      QATR  RR+Y+  L  +A+E+++  + +  
Sbjct: 242 FPNMFPLPTGQSFGGLSMMPIQAM----TQQATRHARRVYVGGLSPTANEQSVATFFSQV 301

Query: 461 LISPGVNRTEGTQPCISCILHKDRGQALVEFLTPDHASAALSFDGSDFSGSILKIRRPKD 520
           + + G N        ++  ++ ++  A VE  + + AS A+S DG  F G+ +K+RRP D
Sbjct: 302 MAAVGGNTAGPGDAVVNVYINHEKKFAFVEMRSVEEASNAMSLDGIIFEGAPVKVRRPSD 361

Query: 521 YVEIVTGDLDKS---------VVVVNEISDVVEDSPNKIIVAGISNRISSEMLRDIVTAF 580
           Y   +   L  S          V +   +    + P++I V G+    +   +R+++ +F
Sbjct: 362 YNPSLAATLGPSQPSPHLNLAAVGLTPGASGGLEGPDRIFVGGLPYYFTESQVRELLESF 421

Query: 581 GPLKAYHFEINNEL--NEPCAFLEYDDQSVMPKACAGLNGLKIGGGVLKVFPAVPFAPLE 640
           G LK +    + E   ++  AF  Y D SV   ACA LNG+K+G   L V  A     L+
Sbjct: 422 GGLKGFDLVKDRETGNSKGYAFCVYQDLSVTDIACAALNGIKMGDKTLTVRRANQGTMLQ 481

Query: 641 RNGWQPCYVIPEH---VKPLLQQP----TAVLKVNNVFNADVLSVLSESDIEEVLEDIRF 691
           +   +   +  +     + ++ QP    T V+ +  V   D L    + +  +++ED+R 
Sbjct: 482 KPEQENVLLHAQQQIAFQRVMLQPGAVATTVVCLTQVVTEDELR--DDEEYGDIMEDMRQ 516

BLAST of Sed0025551 vs. TAIR 10
Match: AT4G36690.2 (U2 snRNP auxilliary factor, large subunit, splicing factor )

HSP 1 Score: 90.1 bits (222), Expect = 9.9e-18
Identity = 140/546 (25.64%), Postives = 236/546 (43.22%), Query Frame = 0

Query: 161 SRFEIENENG----KRHSKDAGGKDRH-EDRAKGKIERESKRKYEHGEDDRNRDKHIPKR 220
           S FE    NG      + ++ GG+D   ED+   K +RES R +E  E  R++D+   K 
Sbjct: 2   SEFEDHEGNGTVADAIYDEENGGRDGEIEDQLDSKPKRES-RDHER-ETSRSKDREREKG 61

Query: 221 HDHDKHHDLENRERKETKISSSSHYEDSKLKRRRKRSQDRESKHKRSGSRSPRPPKHSTK 280
            D D+  D E        +S  S   D +  + R R +DR+  H+    RS R   HS +
Sbjct: 62  RDKDRERDSE--------VSRRSRDRDGEKSKERSRDKDRD--HRERHHRSSRHRDHSRE 121

Query: 281 LARQNELPLDSHVNKSGRWISDSDKTRDFTNSSSNQYKRHSGSTSGLGGYSPRKRRTESA 340
              + E        + GR   D D  R   +   ++ +   G        S  K R+E  
Sbjct: 122 RGERRE--------RGGR---DDDDYRRSRDRDHDRRRDDRGGRRSRRSRSRSKDRSERR 181

Query: 341 VKTPSPVQSLEKKKEVS--DLHPLEKVGLLPGSA-TSNFQLSNPIISLGISNDQSGGALF 400
            ++ SP +S   K+ VS  D+ P     L  G+A T     + P +          GA  
Sbjct: 182 TRSRSPSKS---KQRVSGFDMAPPASAMLAAGAAVTGQVPPAPPTLP---------GAGM 241

Query: 401 SPSMGKPLLEVSSNNLATKTNASLDLIQLTQATRPVRRLYIENLPHSASEKAIIDYLNDF 460
            P+M       S   L+     ++      QATR  RR+Y+  L  +A+E+++  + +  
Sbjct: 242 FPNMFPLPTGQSFGGLSMMPIQAM----TQQATRHARRVYVGGLSPTANEQSVATFFSQV 301

Query: 461 LISPGVNRTEGTQPCISCILHKDRGQALVEFLTPDHASAALSFDGSDFSGSILKIRRPKD 520
           + + G N        ++  ++ ++  A VE  + + AS A+S DG  F G+ +K+RRP D
Sbjct: 302 MAAVGGNTAGPGDAVVNVYINHEKKFAFVEMRSVEEASNAMSLDGIIFEGAPVKVRRPSD 361

Query: 521 YVEIVTGDLDKS---------VVVVNEISDVVEDSPNKIIVAGISNRISSEMLRDIVTAF 580
           Y   +   L  S          V +   +    + P++I V G+    +   +R+++ +F
Sbjct: 362 YNPSLAATLGPSQPSPHLNLAAVGLTPGASGGLEGPDRIFVGGLPYYFTESQVRELLESF 421

Query: 581 GPLKAYHFEINNEL--NEPCAFLEYDDQSVMPKACAGLNGLKIGGGVLKVFPAVPFAPLE 640
           G LK +    + E   ++  AF  Y D SV   ACA LNG+K+G   L V  A     L+
Sbjct: 422 GGLKGFDLVKDRETGNSKGYAFCVYQDLSVTDIACAALNGIKMGDKTLTVRRANQGTMLQ 481

Query: 641 RNGWQPCYVIPEH---VKPLLQQP----TAVLKVNNVFNADVLSVLSESDIEEVLEDIRF 681
           +   +   +  +     + ++ QP    T V+ +  V   D L    + +  +++ED+R 
Sbjct: 482 KPEQENVLLHAQQQIAFQRVMLQPGAVATTVVCLTQVVTEDELR--DDEEYGDIMEDMRQ 506