Sed0025336 (gene) Chayote v1

BLAST of Sed0025336 vs. NCBI nr
Match: XP_038897143.1 (protein SRG1-like [Benincasa hispida])

HSP 1 Score: 557.4 bits (1435), Expect = 9.1e-155
Identity = 271/356 (76.12%), Postives = 303/356 (85.11%), Query Frame = 0

Query: 5   EAKKNNKSRYGSSILVPCVQELAKQPIIEVPPRYLR-----HFQIAASASEIPVIDMSKL 64
           E    N SRYGSSILVP VQELAKQPI E+PPRY+R     H  IA++ASEIPVIDMS  
Sbjct: 3   EENSTNTSRYGSSILVPSVQELAKQPITEIPPRYIRPDLLHHVPIASAASEIPVIDMSNF 62

Query: 65  LSDSATDSELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKLWQME 124
            SD+  DSELARLHSAC+NWGFFQLV HGV DS+IE MKIE ++LFDL +EEKKKLWQ E
Sbjct: 63  HSDATIDSELARLHSACKNWGFFQLVNHGVSDSLIEKMKIETKKLFDLPIEEKKKLWQRE 122

Query: 125 GDVEGFGQAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDL 184
           GDVEGFGQAFV SEEQKLDWSD+FF+AT+P+HLRK RLFQNLPL+FR  +E+YSAEVKDL
Sbjct: 123 GDVEGFGQAFVVSEEQKLDWSDLFFIATSPSHLRKQRLFQNLPLTFRKTLEDYSAEVKDL 182

Query: 185 AAAILRQFERALKITDGELTEMFTDGNQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILL 244
           AAAI    E+ L I  GEL EMF DGNQ+MRMNYYP CP+PEK IG+TPHSDAVG TILL
Sbjct: 183 AAAIFEGVEKVLGIKKGELKEMFKDGNQSMRMNYYPRCPEPEKAIGLTPHSDAVGLTILL 242

Query: 245 QINQVEGLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSI 304
           QIN+VEGLKIKKDGNWI V PLPNAF+VN+GD+MEM+TNG+YKSIEHCATVNSKSERLSI
Sbjct: 243 QINEVEGLKIKKDGNWIPVMPLPNAFVVNVGDIMEMVTNGEYKSIEHCATVNSKSERLSI 302

Query: 305 ATFNNPRFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRIH 356
           ATFNNP  +KEI+   SLIT HSPPLFKTLTYQEFV GLF ++L+GKSYLD MRIH
Sbjct: 303 ATFNNPSMEKEIRPIPSLITPHSPPLFKTLTYQEFVKGLFTQKLDGKSYLDAMRIH 358

BLAST of Sed0025336 vs. NCBI nr
Match: KAG6591778.1 (Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 553.9 bits (1426), Expect = 1.0e-153
Identity = 272/357 (76.19%), Postives = 310/357 (86.83%), Query Frame = 0

Query: 6   AKKNNKSRYGSSILVPCVQELAKQPIIEVPPRYLR----HFQIAASASEIPVIDMSKLLS 65
           A+KNN SR+G SILVP VQELAKQPIIEVP RY+R     F I ++ASEIPVIDMSK  S
Sbjct: 2   AEKNNTSRFGGSILVPSVQELAKQPIIEVPSRYIRPDLHSFPIVSAASEIPVIDMSKFHS 61

Query: 66  DSATDSELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKK-LWQMEG 125
           D++  SELARLHSAC+NWGFFQLV HGV DS+IE MK E Q+ F+L +EEKKK LWQ E 
Sbjct: 62  DASMHSELARLHSACKNWGFFQLVNHGVSDSLIEKMKAETQKFFNLPIEEKKKRLWQSEE 121

Query: 126 DVEGFGQAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLA 185
           D EGFGQAFV SEEQKLDW D+FF+ATAP HLRKPRLFQNLPL+FRN MEEYSAEVK+LA
Sbjct: 122 DAEGFGQAFVVSEEQKLDWCDLFFIATAPPHLRKPRLFQNLPLAFRNTMEEYSAEVKNLA 181

Query: 186 AAILRQFERALKITDGELTEMFTDGNQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQ 245
            AI  QFE+AL I +GELTE+F DG+Q+MRMNYYPPCP+PEKVIG+TPHSDAVG TILLQ
Sbjct: 182 MAIFEQFEKALGIKEGELTEIFKDGSQSMRMNYYPPCPEPEKVIGLTPHSDAVGLTILLQ 241

Query: 246 INQVEGLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIA 305
           IN++EGLKIKKDGNW+AVTPLPNAFIVNIGD+ME+ITNG+YKSIEHCATVN+K ERLSIA
Sbjct: 242 INEMEGLKIKKDGNWVAVTPLPNAFIVNIGDIMEIITNGEYKSIEHCATVNAKRERLSIA 301

Query: 306 TFNNPRFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRIHRP 358
           TFNNP  +KEIK+  SLIT HSPPLF TLTYQ+FV  LF+R+L+GKS+LDT+RIH P
Sbjct: 302 TFNNPSLEKEIKAIPSLITPHSPPLFTTLTYQQFVRLLFSRKLDGKSHLDTLRIHTP 358

BLAST of Sed0025336 vs. NCBI nr
Match: XP_022935942.1 (protein SRG1-like [Cucurbita moschata])

HSP 1 Score: 553.1 bits (1424), Expect = 1.7e-153
Identity = 272/357 (76.19%), Postives = 309/357 (86.55%), Query Frame = 0

Query: 6   AKKNNKSRYGSSILVPCVQELAKQPIIEVPPRYLR----HFQIAASASEIPVIDMSKLLS 65
           A+KNN SR+G SILVP VQELAKQPIIEVP RY+R     F I ++ASEIPVIDMS   S
Sbjct: 2   AEKNNTSRFGGSILVPSVQELAKQPIIEVPSRYIRPDLHSFPIVSAASEIPVIDMSNFHS 61

Query: 66  DSATDSELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKK-LWQMEG 125
           D++  SELARLHSAC+NWGFFQLV HGV DS+IE MK E Q  F+L +EEKKK LWQ E 
Sbjct: 62  DASMHSELARLHSACKNWGFFQLVNHGVSDSLIEKMKAETQMFFNLPIEEKKKRLWQSEE 121

Query: 126 DVEGFGQAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLA 185
           D EGFGQAFV SEEQKLDW D+FF+ATAP HLRKPRLFQNLPL+FRN MEEYSAEVK+LA
Sbjct: 122 DAEGFGQAFVVSEEQKLDWCDLFFIATAPPHLRKPRLFQNLPLAFRNTMEEYSAEVKNLA 181

Query: 186 AAILRQFERALKITDGELTEMFTDGNQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQ 245
            AI  QFE+AL I +GELTE+F DG+Q+MRMNYYPPCP+PEKVIG+TPHSDAVG TILLQ
Sbjct: 182 MAIFEQFEKALGIKEGELTEIFKDGSQSMRMNYYPPCPEPEKVIGLTPHSDAVGLTILLQ 241

Query: 246 INQVEGLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIA 305
           IN++EGLKIKKDGNW+AVTPLPNAFIVNIGD+ME+ITNG+YKSIEHCATVN+K ERLSIA
Sbjct: 242 INEMEGLKIKKDGNWVAVTPLPNAFIVNIGDIMEIITNGEYKSIEHCATVNAKRERLSIA 301

Query: 306 TFNNPRFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRIHRP 358
           TFNNPR +KEIK+  SLIT HSPPLF TLTYQ+FV  LF+R+L+GKS+LDT+RIH P
Sbjct: 302 TFNNPRLEKEIKAIPSLITPHSPPLFTTLTYQQFVRLLFSRKLDGKSHLDTLRIHTP 358

BLAST of Sed0025336 vs. NCBI nr
Match: KAG7024656.1 (Protein SRG1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 551.6 bits (1420), Expect = 5.0e-153
Identity = 271/357 (75.91%), Postives = 309/357 (86.55%), Query Frame = 0

Query: 6   AKKNNKSRYGSSILVPCVQELAKQPIIEVPPRYLR----HFQIAASASEIPVIDMSKLLS 65
           A+KNN SR+G SILVP VQELAKQPIIEVP RY+R     F I ++ASEIPVIDMSK  S
Sbjct: 349 AEKNNTSRFGGSILVPSVQELAKQPIIEVPSRYIRPDLHSFPIVSAASEIPVIDMSKFHS 408

Query: 66  DSATDSELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKK-LWQMEG 125
           D++  SELARLHSAC+NWGFFQLV HGV DS+IE MK E Q+ F+L +EEKKK LWQ E 
Sbjct: 409 DASMHSELARLHSACKNWGFFQLVNHGVSDSLIEKMKAETQKFFNLPIEEKKKRLWQSEE 468

Query: 126 DVEGFGQAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLA 185
           D EGFGQAFV SEEQKLDW D+FF+ATAP HLRKPRLFQNLPL+FRN MEEYSAEVK+LA
Sbjct: 469 DAEGFGQAFVVSEEQKLDWCDLFFIATAPPHLRKPRLFQNLPLAFRNTMEEYSAEVKNLA 528

Query: 186 AAILRQFERALKITDGELTEMFTDGNQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQ 245
            AI  QFE+AL I +GELTE+F DG+Q+MR NYYPPCP+PEKVIG+TPHSDAVG TILLQ
Sbjct: 529 MAIFEQFEKALGIKEGELTEIFKDGSQSMRTNYYPPCPEPEKVIGLTPHSDAVGLTILLQ 588

Query: 246 INQVEGLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIA 305
           IN++EGLKIKKDGNW+AVTPLPNAFIVNIGD+ME+ITNG+YKSIEHCATVN+K ERLSIA
Sbjct: 589 INEMEGLKIKKDGNWVAVTPLPNAFIVNIGDIMEIITNGEYKSIEHCATVNAKRERLSIA 648

Query: 306 TFNNPRFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRIHRP 358
           TFNNP  +KEIK+  SLIT HSPPLF TLTYQ+FV  LF+R+L+GKS+LDT+RIH P
Sbjct: 649 TFNNPSLEKEIKAIPSLITPHSPPLFTTLTYQQFVRLLFSRKLDGKSHLDTLRIHTP 705

BLAST of Sed0025336 vs. NCBI nr
Match: XP_008463796.1 (PREDICTED: probable 2-oxoglutarate/Fe(II)-dependent dioxygenase [Cucumis melo])

HSP 1 Score: 538.5 bits (1386), Expect = 4.4e-149
Identity = 261/354 (73.73%), Postives = 299/354 (84.46%), Query Frame = 0

Query: 7   KKNNKSRYGSSILVPCVQELAKQPIIEVPPRY-----LRHFQIAASASEIPVIDMSKLLS 66
           +KN+  RYGSSILVP VQELAK PI E+P RY     L H  I++ AS+IPVIDMS L S
Sbjct: 4   EKNDTLRYGSSILVPSVQELAKHPITEIPHRYIHPDLLHHSPISSDASQIPVIDMSNLRS 63

Query: 67  DSATDSELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKLWQMEGD 126
               DSEL+RLHSAC+NWGFFQLVKHGV DS++E MK+E Q+LF+L +EEKKKLWQ EGD
Sbjct: 64  HDTMDSELSRLHSACKNWGFFQLVKHGVSDSLVERMKMETQKLFELPIEEKKKLWQREGD 123

Query: 127 VEGFGQAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLAA 186
           VEGFGQAFV SEEQKLDW DIFF+AT P H RKPRLFQNLPLS R  +EEYSA VKD+AA
Sbjct: 124 VEGFGQAFVTSEEQKLDWCDIFFIATLPLHFRKPRLFQNLPLSLRETLEEYSAAVKDVAA 183

Query: 187 AILRQFERALKITDGELTEMFTDGNQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQI 246
            IL   E+AL I +GEL+E+F DGNQ+MRMNYYPPCP+PEKVIG+TPHSDA G TILLQI
Sbjct: 184 EILDGVEKALGIKEGELSELFKDGNQSMRMNYYPPCPEPEKVIGLTPHSDAAGLTILLQI 243

Query: 247 NQVEGLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIAT 306
           N+VEGLKIKK+GNWI V PLPNAFIVNIGD++EM+TNG+YKSIEHCATVNSKSERLSIAT
Sbjct: 244 NKVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGEYKSIEHCATVNSKSERLSIAT 303

Query: 307 FNNPRFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRIH 356
           FN+P  +KEI+   SLIT HSPPLF TLTYQE+V G+F+R+L+GKSYLD MRIH
Sbjct: 304 FNSPSLEKEIRPTPSLITPHSPPLFTTLTYQEYVRGIFSRKLDGKSYLDAMRIH 357

BLAST of Sed0025336 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 3.7e-106
Identity = 191/349 (54.73%), Postives = 245/349 (70.20%), Query Frame = 0

Query: 16  SSILVPCVQELAKQ-PIIEVPPRYLRHFQIAASAS-------EIPVIDMSKLLSDSATDS 75
           SSILVP VQE+ K+  I  VPPRY+R  Q             EIP+IDM +L S +  DS
Sbjct: 10  SSILVPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDS 69

Query: 76  ELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKLWQMEGDVEGFGQ 135
           E+ +L  AC+ WGFFQLV HG+  S ++ +K E Q  F+L MEEKKK WQ   ++EGFGQ
Sbjct: 70  EVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQ 129

Query: 136 AFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLAAAILRQF 195
           AFV SE+QKLDW+D+FF    P  LRKP LF  LPL FR+ +E YS+EV+ +A  ++ + 
Sbjct: 130 AFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKM 189

Query: 196 ERALKITDGELTEMF--TDGNQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQINQVE 255
            RAL+I   EL ++F   D  Q+MRMNYYPPCP P++VIG+TPHSD+VG T+L+Q+N VE
Sbjct: 190 ARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVE 249

Query: 256 GLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIATFNNP 315
           GL+IKKDG W+ V PLPNAFIVNIGD++E+ITNG Y+SIEH   VNS+ ERLSIATF+N 
Sbjct: 250 GLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNV 309

Query: 316 RFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRI 355
              KE+    SL+       FK LT +E+  GLF+R L+GK+YLD +RI
Sbjct: 310 GMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of Sed0025336 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.2e-101
Identity = 185/357 (51.82%), Postives = 250/357 (70.03%), Query Frame = 0

Query: 13  RYGSSILVPCVQELAKQPIIEVPPRYL---RHFQIAASAS------EIPVIDMSKLLSDS 72
           + G+ + +P VQELAK  + E+P RY+    + Q+   AS       +PVID+  L+S  
Sbjct: 8   KLGNGMSIPSVQELAKLTLAEIPSRYICTVENLQLPVGASVIDDHETVPVIDIENLISSE 67

Query: 73  ATDS--ELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKLWQMEGD 132
                 EL RLHSAC+ WGFFQ+V HGV  S+++++K + Q  F+L M EK K  Q +GD
Sbjct: 68  PVTEKLELDRLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLSMNEKIKYGQKDGD 127

Query: 133 VEGFGQAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLAA 192
           VEGFGQAFVASE+Q LDW+DIF + T P HLRKP LF  LPL  R  +E YS+E+K L+ 
Sbjct: 128 VEGFGQAFVASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRETIESYSSEMKKLSM 187

Query: 193 AILRQFERALKITD---GELTEMFTDGNQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTIL 252
            +  + E+AL++      E++E+F D  Q MRMNYYPPCP PE  IG+TPHSD  G TIL
Sbjct: 188 VLFEKMEKALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGLTPHSDFGGLTIL 247

Query: 253 LQINQVEGLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLS 312
           LQ+N+VEGL+IK +G WI+V PLPNAF+VN+GD++E++TNG Y+S++H A VNS  ERLS
Sbjct: 248 LQLNEVEGLQIKNEGRWISVKPLPNAFVVNVGDVLEIMTNGMYRSVDHRAVVNSTKERLS 307

Query: 313 IATFNNPRFDKEIKSPSSLITHHSPPLFKT-LTYQEFVSGLFARELNGKSYLDTMRI 355
           IATF++P  + EI   SSLIT ++P LF++  TY E V    +R+L+GKS+LD+MR+
Sbjct: 308 IATFHDPNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSRKLDGKSFLDSMRM 364

BLAST of Sed0025336 vs. ExPASy Swiss-Prot
Match: D4N500 (Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 1.8e-97
Identity = 184/363 (50.69%), Postives = 246/363 (67.77%), Query Frame = 0

Query: 7   KKNNKSRYGSSILVPCVQELAKQPIIEVPPRYL---RHFQIAASAS------EIPVIDMS 66
           +K    + G+ + +P VQELAK  + E+P RY+    +  +   AS       IPVID+ 
Sbjct: 2   EKAKLMKLGNGMEIPSVQELAKLTLAEIPSRYVCANENLLLPMGASVINDHETIPVIDIE 61

Query: 67  KLLSDSAT--DSELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKL 126
            LLS        EL RLH AC+ WGFFQ+V HGV  S+++S+K E Q  F+L M+EK K 
Sbjct: 62  NLLSPEPIIGKLELDRLHFACKEWGFFQVVNHGVDASLVDSVKSEIQGFFNLSMDEKTKY 121

Query: 127 WQMEGDVEGFGQAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAE 186
            Q +GDVEGFGQ F+ SE+Q LDW+DIF + T P HLRKP LF  LP+  R  +E YS+E
Sbjct: 122 EQEDGDVEGFGQGFIESEDQTLDWADIFMMFTLPLHLRKPHLFSKLPVPLRETIESYSSE 181

Query: 187 VKDLAAAILRQFERALKITDGE---LTEMFTDGNQTMRMNYYPPCPDPEKVIGVTPHSDA 246
           +K L+  +  + E+AL++   E   ++E+F DG Q MRMNYYPPCP P   IG+T HSD 
Sbjct: 182 MKKLSMVLFNKMEKALQVQAAEIKGMSEVFIDGTQAMRMNYYPPCPQPNLAIGLTSHSDF 241

Query: 247 VGFTILLQINQVEGLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNS 306
            G TILLQIN+VEGL+IK++G WI+V PLPNAF+VN+GD++E++TNG Y S++H A VNS
Sbjct: 242 GGLTILLQINEVEGLQIKREGTWISVKPLPNAFVVNVGDILEIMTNGIYHSVDHRAVVNS 301

Query: 307 KSERLSIATFNNPRFDKEIKSPSSLITHHSPPLFKT-LTYQEFVSGLFARELNGKSYLDT 355
            +ERLSIATF++P  +  I   SSLIT  +P LFK+  TY + V     R+L+GKS+LD+
Sbjct: 302 TNERLSIATFHDPSLESVIGPISSLITPETPALFKSGSTYGDLVEECKTRKLDGKSFLDS 361

BLAST of Sed0025336 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 1.1e-94
Identity = 170/353 (48.16%), Postives = 246/353 (69.69%), Query Frame = 0

Query: 13  RYGSSILVPCVQELAKQPIIEVPPRYLRHFQ-----IAASASE---IPVIDMSKLLSDSA 72
           + G+ + +P VQELAK  + E+P RY    +     I AS ++   +PVID+  LLS   
Sbjct: 8   KLGNGLSIPSVQELAKLTLAEIPSRYTCTGESPLNNIGASVTDDETVPVIDLQNLLSPEP 67

Query: 73  T--DSELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKLWQMEGDV 132
                EL +LHSAC+ WGFFQLV HGV   +++++K E +  F+L M EK K  Q +GD 
Sbjct: 68  VVGKLELDKLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDGDF 127

Query: 133 EGFGQAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLAAA 192
           EGFGQ ++ SE+Q+LDW+++F + + P HLRKP LF  LPL FR  +E Y +++K L+  
Sbjct: 128 EGFGQPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETLESYLSKMKKLSTV 187

Query: 193 ILRQFERALKITDGE-LTEMFTDGNQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQI 252
           +    E++L++ + + +T++F DG QTMRMNYYPPCP PE V+G+T HSD  G TILLQ+
Sbjct: 188 VFEMLEKSLQLVEIKGMTDLFEDGLQTMRMNYYPPCPRPELVLGLTSHSDFSGLTILLQL 247

Query: 253 NQVEGLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIAT 312
           N+VEGL+I+K+  WI++ PLP+AFIVN+GD++E++TNG Y+S+EH A VNS  ERLSIAT
Sbjct: 248 NEVEGLQIRKEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTKERLSIAT 307

Query: 313 FNNPRFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRI 355
           F++ + + EI   SSL+T  +P LFK   Y++ +    +R+L+GKS+LD MR+
Sbjct: 308 FHDSKLESEIGPISSLVTPETPALFKRGRYEDILKENLSRKLDGKSFLDYMRM 360

BLAST of Sed0025336 vs. ExPASy Swiss-Prot
Match: A2A1A0 (S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 8.5e-71
Identity = 145/352 (41.19%), Postives = 216/352 (61.36%), Query Frame = 0

Query: 10  NKSRYGSSILVPCVQELAKQPIIEVPPRYLR----HFQIAA--SASEIPVIDMSKLLSDS 69
           N +  G S+ V  VQ LA + +  +P RY+R    H  +    ++ EIPVID+S+LL   
Sbjct: 4   NLTGVGGSLPVENVQVLAGKELKNLPNRYVRPELEHDDVVPIDNSLEIPVIDLSRLLDQQ 63

Query: 70  ATDSELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKLWQMEGDVE 129
               ELA+ HSAC +WGFFQL+ HGVR+ VIE MK++ +  F L  +EK    Q+   +E
Sbjct: 64  YACDELAKFHSACLDWGFFQLINHGVREEVIEKMKVDTEDFFRLPFKEKNAYRQLPNGME 123

Query: 130 GFGQAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLAAAI 189
           G+GQAFV SEEQKLDW+D+ FL T P   R  R +   P SFR  ME+YS E++ +A  +
Sbjct: 124 GYGQAFVTSEEQKLDWADMHFLITKPVQERNMRFWPTSPTSFRETMEKYSMELQKVAMCL 183

Query: 190 LRQFERALKITDGELTE-MFTDGNQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQIN 249
                + L +    LT+ + T  N+   +   P      + +G++PHSDA G T+L+Q+N
Sbjct: 184 TGMMAKNLGLESEILTKPLRTVFNREDEL--LPSMSSCGEGLGLSPHSDATGLTLLIQVN 243

Query: 250 QVEGLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIATF 309
           +V GL IKKD  W+ + P+  AF+VNIGD++E+++NG YKSIEH A +N+  ERLSIA F
Sbjct: 244 EVNGLHIKKDEKWVPIKPILGAFVVNIGDVIEIMSNGIYKSIEHRAVINTDKERLSIAAF 303

Query: 310 NNPRFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRI 355
           ++P +  +I     L+  +    +KT+ Y++++      +L+GKS LD M++
Sbjct: 304 HDPEYGTKIGPLPDLVKENGVK-YKTIDYEDYLIRSSNIKLDGKSLLDQMKL 352

BLAST of Sed0025336 vs. ExPASy TrEMBL
Match: A0A6J1F704 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111442702 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 8.3e-154
Identity = 272/357 (76.19%), Postives = 309/357 (86.55%), Query Frame = 0

Query: 6   AKKNNKSRYGSSILVPCVQELAKQPIIEVPPRYLR----HFQIAASASEIPVIDMSKLLS 65
           A+KNN SR+G SILVP VQELAKQPIIEVP RY+R     F I ++ASEIPVIDMS   S
Sbjct: 2   AEKNNTSRFGGSILVPSVQELAKQPIIEVPSRYIRPDLHSFPIVSAASEIPVIDMSNFHS 61

Query: 66  DSATDSELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKK-LWQMEG 125
           D++  SELARLHSAC+NWGFFQLV HGV DS+IE MK E Q  F+L +EEKKK LWQ E 
Sbjct: 62  DASMHSELARLHSACKNWGFFQLVNHGVSDSLIEKMKAETQMFFNLPIEEKKKRLWQSEE 121

Query: 126 DVEGFGQAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLA 185
           D EGFGQAFV SEEQKLDW D+FF+ATAP HLRKPRLFQNLPL+FRN MEEYSAEVK+LA
Sbjct: 122 DAEGFGQAFVVSEEQKLDWCDLFFIATAPPHLRKPRLFQNLPLAFRNTMEEYSAEVKNLA 181

Query: 186 AAILRQFERALKITDGELTEMFTDGNQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQ 245
            AI  QFE+AL I +GELTE+F DG+Q+MRMNYYPPCP+PEKVIG+TPHSDAVG TILLQ
Sbjct: 182 MAIFEQFEKALGIKEGELTEIFKDGSQSMRMNYYPPCPEPEKVIGLTPHSDAVGLTILLQ 241

Query: 246 INQVEGLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIA 305
           IN++EGLKIKKDGNW+AVTPLPNAFIVNIGD+ME+ITNG+YKSIEHCATVN+K ERLSIA
Sbjct: 242 INEMEGLKIKKDGNWVAVTPLPNAFIVNIGDIMEIITNGEYKSIEHCATVNAKRERLSIA 301

Query: 306 TFNNPRFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRIHRP 358
           TFNNPR +KEIK+  SLIT HSPPLF TLTYQ+FV  LF+R+L+GKS+LDT+RIH P
Sbjct: 302 TFNNPRLEKEIKAIPSLITPHSPPLFTTLTYQQFVRLLFSRKLDGKSHLDTLRIHTP 358

BLAST of Sed0025336 vs. ExPASy TrEMBL
Match: A0A1S3CK11 (probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo OX=3656 GN=LOC103501850 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 2.1e-149
Identity = 261/354 (73.73%), Postives = 299/354 (84.46%), Query Frame = 0

Query: 7   KKNNKSRYGSSILVPCVQELAKQPIIEVPPRY-----LRHFQIAASASEIPVIDMSKLLS 66
           +KN+  RYGSSILVP VQELAK PI E+P RY     L H  I++ AS+IPVIDMS L S
Sbjct: 4   EKNDTLRYGSSILVPSVQELAKHPITEIPHRYIHPDLLHHSPISSDASQIPVIDMSNLRS 63

Query: 67  DSATDSELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKLWQMEGD 126
               DSEL+RLHSAC+NWGFFQLVKHGV DS++E MK+E Q+LF+L +EEKKKLWQ EGD
Sbjct: 64  HDTMDSELSRLHSACKNWGFFQLVKHGVSDSLVERMKMETQKLFELPIEEKKKLWQREGD 123

Query: 127 VEGFGQAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLAA 186
           VEGFGQAFV SEEQKLDW DIFF+AT P H RKPRLFQNLPLS R  +EEYSA VKD+AA
Sbjct: 124 VEGFGQAFVTSEEQKLDWCDIFFIATLPLHFRKPRLFQNLPLSLRETLEEYSAAVKDVAA 183

Query: 187 AILRQFERALKITDGELTEMFTDGNQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQI 246
            IL   E+AL I +GEL+E+F DGNQ+MRMNYYPPCP+PEKVIG+TPHSDA G TILLQI
Sbjct: 184 EILDGVEKALGIKEGELSELFKDGNQSMRMNYYPPCPEPEKVIGLTPHSDAAGLTILLQI 243

Query: 247 NQVEGLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIAT 306
           N+VEGLKIKK+GNWI V PLPNAFIVNIGD++EM+TNG+YKSIEHCATVNSKSERLSIAT
Sbjct: 244 NKVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGEYKSIEHCATVNSKSERLSIAT 303

Query: 307 FNNPRFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRIH 356
           FN+P  +KEI+   SLIT HSPPLF TLTYQE+V G+F+R+L+GKSYLD MRIH
Sbjct: 304 FNSPSLEKEIRPTPSLITPHSPPLFTTLTYQEYVRGIFSRKLDGKSYLDAMRIH 357

BLAST of Sed0025336 vs. ExPASy TrEMBL
Match: A0A6J1BUE5 (protein SRG1-like OS=Momordica charantia OX=3673 GN=LOC111004846 PE=3 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 2.0e-147
Identity = 263/358 (73.46%), Postives = 301/358 (84.08%), Query Frame = 0

Query: 4   KEAKKNNKSRYGSSILVPCVQELAKQPIIEVPPRYLR----HFQIAASASEIPVIDMSKL 63
           K  +KN  S YGSSI VP VQELAKQP IE+PPRY+R       I ++ASEIPVID + L
Sbjct: 40  KNMEKNASSTYGSSIPVPSVQELAKQPFIEIPPRYVRSDLLRSPIVSAASEIPVIDFASL 99

Query: 64  LSDSATDSELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKLWQME 123
           LS ++ DSE ARLHSAC +WGFFQLV HGV+   IE M+IE Q  F L +EE+KKLWQ+E
Sbjct: 100 LSGASMDSESARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEERKKLWQIE 159

Query: 124 GDVEGFGQAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDL 183
           GDVEGFGQAFV SEEQKLDWSDIFFL TAP H RKPRLFQNLPLSFRNA+E+YS+EVKD+
Sbjct: 160 GDVEGFGQAFVVSEEQKLDWSDIFFLCTAPEHQRKPRLFQNLPLSFRNALEDYSSEVKDV 219

Query: 184 AAAILRQFERALKITDGELTEMFTDGNQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILL 243
           AAAI+R+ ERAL+I +GE+TEMF DGNQ+MRMNYYP CP+ EKVIG+TPHSDAVG TILL
Sbjct: 220 AAAIVRELERALEIKEGEMTEMFKDGNQSMRMNYYPACPETEKVIGLTPHSDAVGLTILL 279

Query: 244 QINQVEGLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSI 303
           QINQVEGLKIKK+G WI V PLP+AFIVNIGD+MEMITNGKY+SIEHCATVNS+SERLSI
Sbjct: 280 QINQVEGLKIKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSI 339

Query: 304 ATFNNPRFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRIHRP 358
           ATF NP F+K I+   SLIT HSPPLF +LTYQ +VSGLF R+L+GKSYLD MRI+ P
Sbjct: 340 ATFCNPSFEKLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINHP 394

BLAST of Sed0025336 vs. ExPASy TrEMBL
Match: A0A067JV41 (Fe2OG dioxygenase domain-containing protein OS=Jatropha curcas OX=180498 GN=JCGZ_27165 PE=3 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 9.9e-123
Identity = 221/352 (62.78%), Postives = 266/352 (75.57%), Query Frame = 0

Query: 12  SRYGSSILVPCVQELAKQPIIEVPPRYLR----HFQIAA----SASEIPVIDMSKLLSDS 71
           S  GSS+LVP VQELAK+P++ VP RY+R    H  ++A    S  +IPVIDMSKL    
Sbjct: 6   SSLGSSLLVPSVQELAKEPLVSVPQRYIRPDQNHSIVSANEDGSLPDIPVIDMSKLTHQE 65

Query: 72  ATDSELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKLWQMEGDVE 131
           + DSELA+LHSAC  WGFFQLV HGV  S++E MKI+ Q  FDL +EEKKK WQ  G+VE
Sbjct: 66  SMDSELAKLHSACTEWGFFQLVNHGVSSSLLEEMKIQVQEFFDLPVEEKKKFWQYPGEVE 125

Query: 132 GFGQAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLAAAI 191
           GFGQ+FV SEEQKLDWSD+FF+ T P HLRKP L   LPL FR  +E YS EVK+LAAAI
Sbjct: 126 GFGQSFVVSEEQKLDWSDMFFMVTQPVHLRKPHLLPKLPLPFRETVENYSLEVKNLAAAI 185

Query: 192 LRQFERALKITDGELTEMFTDG-NQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQIN 251
           L Q  + LKI   E+ E+FT G  QTMRMNYYPPCP+P+KV+G+TPHSD  G TILLQ++
Sbjct: 186 LEQMAKVLKIRVEEMRELFTQGIRQTMRMNYYPPCPEPDKVLGITPHSDGTGLTILLQVS 245

Query: 252 QVEGLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIATF 311
            VEGL+I KDGNWI V PLPNAF+VNIGD++E+ITNG Y+SI H ATVN K +RLSIA+F
Sbjct: 246 DVEGLQIMKDGNWIPVKPLPNAFVVNIGDILEIITNGAYRSILHRATVNCKEKRLSIASF 305

Query: 312 NNPRFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRI 355
           ++PRFD EI    SLI   +P LF+ +T Q+F  GLF+REL  KSYLDT+RI
Sbjct: 306 HSPRFDGEIYPAPSLINEETPALFRRVTVQQFFKGLFSRELRAKSYLDTLRI 357

BLAST of Sed0025336 vs. ExPASy TrEMBL
Match: A0A2C9UE38 (Fe2OG dioxygenase domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_15G078000 PE=3 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 9.9e-123
Identity = 217/360 (60.28%), Postives = 273/360 (75.83%), Query Frame = 0

Query: 4   KEAKKNNKSRYGSSILVPCVQELAKQPIIEVPPRYLR----HFQIA----ASASEIPVID 63
           K++ ++     GSS+LVPCVQELAK+ ++ VPPRY+R    H  +A    AS  E+PVID
Sbjct: 14  KKSMESKGGSVGSSLLVPCVQELAKEHLVTVPPRYIRPDQDHPIVAAGDDASLLEVPVID 73

Query: 64  MSKLLSDSATDSELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKL 123
           M +L    + DSELA+LHSAC+ WGFFQLV HGV  S++E +K + Q  F+L MEEKKK 
Sbjct: 74  MQRLHDPESMDSELAKLHSACKEWGFFQLVNHGVSSSLLERIKTDIQDFFNLPMEEKKKF 133

Query: 124 WQMEGDVEGFGQAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAE 183
           WQ  G+VEGFGQAFV +EEQKLDW+D+FF+ T P HLRKP LF  LPL FR+ +E YS+E
Sbjct: 134 WQYPGEVEGFGQAFVVAEEQKLDWADLFFMVTQPVHLRKPHLFPKLPLPFRDTLESYSSE 193

Query: 184 VKDLAAAILRQFERALKITDGELTEMFTDG-NQTMRMNYYPPCPDPEKVIGVTPHSDAVG 243
           VK+LA ++L Q  + LK+ D E+ ++FT+G  QTMRMNYYPPCP PEKVIG+TPHSD  G
Sbjct: 194 VKNLAVSVLEQMAKVLKMRDDEMRDLFTEGIRQTMRMNYYPPCPQPEKVIGLTPHSDGTG 253

Query: 244 FTILLQINQVEGLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKS 303
            +ILLQIN VEGL+I KDG WI V PLPNAF+VNIGD+ME+ITNG Y+SI H ATVNS++
Sbjct: 254 LSILLQINDVEGLQIMKDGKWIPVKPLPNAFVVNIGDIMEIITNGTYRSILHRATVNSQT 313

Query: 304 ERLSIATFNNPRFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRI 355
           ERLS+  F++PRFD EI    SLIT  +P LFK +T ++F  GLF+REL  KSYLDT+R+
Sbjct: 314 ERLSVVAFHSPRFDGEISPAPSLITEETPALFKRITVKDFFKGLFSRELRSKSYLDTLRV 373

BLAST of Sed0025336 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 397.1 bits (1019), Expect = 1.5e-110
Identity = 194/348 (55.75%), Postives = 253/348 (72.70%), Query Frame = 0

Query: 16  SSILVPCVQELAKQPIIE--VPPRYLRHFQ------IAASASEIPVIDMSKLLSDSATDS 75
           SS++VP VQE+ K+ +I   +PPRY+R  Q      I +  ++IP+IDMS L S ++ DS
Sbjct: 9   SSVIVPSVQEMVKEKVITTVLPPRYVRSDQEKGEAAIDSGENQIPIIDMSLLSSSTSMDS 68

Query: 76  ELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKLWQMEGDVEGFGQ 135
           E+ +L  AC+ WGFFQLV HG+    ++  K + Q  F+L MEEKKKLWQ  GD+EGFGQ
Sbjct: 69  EIDKLDFACKEWGFFQLVNHGMD---LDKFKSDIQDFFNLPMEEKKKLWQQPGDIEGFGQ 128

Query: 136 AFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLAAAILRQF 195
           AFV SEEQKLDW+D+FFL   P  LRKP LF  LPL FR+ ++ YSAE+K +A  +  + 
Sbjct: 129 AFVFSEEQKLDWADVFFLTMQPVPLRKPHLFPKLPLPFRDTLDTYSAELKSIAKVLFAKL 188

Query: 196 ERALKITDGELTEMFTDG-NQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQINQVEG 255
             ALKI   E+ ++F D   Q +RMNYYPPCP+P+K IG+TPHSDA G TILLQ+N+VEG
Sbjct: 189 ASALKIKPEEMEKLFDDELGQRIRMNYYPPCPEPDKAIGLTPHSDATGLTILLQVNEVEG 248

Query: 256 LKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIATFNNPR 315
           L+IKKDG W++V PLPNA +VN+GD++E+ITNG Y+SIEH   VNS+ ERLS+A+F+N  
Sbjct: 249 LQIKKDGKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVASFHNTG 308

Query: 316 FDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRI 355
           F KEI    SL+  H   LFKTLT +E+  GLF+REL+GK+YLD MRI
Sbjct: 309 FGKEIGPMRSLVERHKGALFKTLTTEEYFHGLFSRELDGKAYLDVMRI 353

BLAST of Sed0025336 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 386.3 bits (991), Expect = 2.6e-107
Identity = 191/349 (54.73%), Postives = 245/349 (70.20%), Query Frame = 0

Query: 16  SSILVPCVQELAKQ-PIIEVPPRYLRHFQIAASAS-------EIPVIDMSKLLSDSATDS 75
           SSILVP VQE+ K+  I  VPPRY+R  Q             EIP+IDM +L S +  DS
Sbjct: 10  SSILVPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDS 69

Query: 76  ELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKLWQMEGDVEGFGQ 135
           E+ +L  AC+ WGFFQLV HG+  S ++ +K E Q  F+L MEEKKK WQ   ++EGFGQ
Sbjct: 70  EVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQ 129

Query: 136 AFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLAAAILRQF 195
           AFV SE+QKLDW+D+FF    P  LRKP LF  LPL FR+ +E YS+EV+ +A  ++ + 
Sbjct: 130 AFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKM 189

Query: 196 ERALKITDGELTEMF--TDGNQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQINQVE 255
            RAL+I   EL ++F   D  Q+MRMNYYPPCP P++VIG+TPHSD+VG T+L+Q+N VE
Sbjct: 190 ARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVE 249

Query: 256 GLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIATFNNP 315
           GL+IKKDG W+ V PLPNAFIVNIGD++E+ITNG Y+SIEH   VNS+ ERLSIATF+N 
Sbjct: 250 GLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNV 309

Query: 316 RFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRI 355
              KE+    SL+       FK LT +E+  GLF+R L+GK+YLD +RI
Sbjct: 310 GMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of Sed0025336 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 384.8 bits (987), Expect = 7.6e-107
Identity = 188/348 (54.02%), Postives = 248/348 (71.26%), Query Frame = 0

Query: 16  SSILVPCVQELAKQPII-EVPPRYLRHFQIAAS-------ASEIPVIDMSKLLSDSATDS 75
           SSI+VP VQE+ K+ +I  VPPRY+R  Q  A         ++IP+IDMS L S ++ DS
Sbjct: 9   SSIIVPSVQEMVKEKMITTVPPRYVRSDQDVAEIAVDSGLRNQIPIIDMSLLCSSTSMDS 68

Query: 76  ELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKLWQMEGDVEGFGQ 135
           E+ +L SAC+ WGFFQLV HG+  S +  +K E Q  F+L MEEKK LWQ   ++EGFGQ
Sbjct: 69  EIDKLDSACKEWGFFQLVNHGMESSFLNKVKSEVQDFFNLPMEEKKNLWQQPDEIEGFGQ 128

Query: 136 AFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLAAAILRQF 195
            FV SEEQKLDW+D+FFL   P  LRKP LF  LPL FR+ ++ YSAEVK +A  +L + 
Sbjct: 129 VFVVSEEQKLDWADMFFLTMQPVRLRKPHLFPKLPLPFRDTLDMYSAEVKSIAKILLGKI 188

Query: 196 ERALKITDGELTEMFTDG-NQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQINQVEG 255
             ALKI   E+ ++F D   Q +R+NYYP CP+P+KVIG+TPHSD+ G TILLQ N+VEG
Sbjct: 189 AVALKIKPEEMDKLFDDELGQRIRLNYYPRCPEPDKVIGLTPHSDSTGLTILLQANEVEG 248

Query: 256 LKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIATFNNPR 315
           L+IKK+  W++V PLPNA +VN+GD++E+ITNG Y+SIEH   VNS+ ERLS+A F+N  
Sbjct: 249 LQIKKNAKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVAAFHNIG 308

Query: 316 FDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRI 355
             KEI    SL+  H    FK++T +E+ +GLF+REL+GK+YLD MR+
Sbjct: 309 LGKEIGPMRSLVERHKAAFFKSVTTEEYFNGLFSRELDGKAYLDVMRL 356

BLAST of Sed0025336 vs. TAIR 10
Match: AT1G17010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 375.2 bits (962), Expect = 6.0e-104
Identity = 190/349 (54.44%), Postives = 245/349 (70.20%), Query Frame = 0

Query: 16  SSILVPCVQELAKQPII-EVPPRYLRHFQIAASA--------SEIPVIDMSKLLSDSATD 75
           SSILVP VQE+ K  +I  VPPRY+R+ Q             SEIP+IDM++L S +A D
Sbjct: 10  SSILVPSVQEMVKDKMITTVPPRYVRYDQDKTEVVVHDSGLISEIPIIDMNRLCSSTAVD 69

Query: 76  SELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKLWQMEGDVEGFG 135
           SE+ +L  AC+ +GFFQLV HG+  S ++ +K E Q  F+L MEEKKKLWQ    +EGFG
Sbjct: 70  SEVEKLDFACKEYGFFQLVNHGIDPSFLDKIKSEIQDFFNLPMEEKKKLWQTPAVMEGFG 129

Query: 136 QAFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLAAAILRQ 195
           QAFV SE+QKLDW+D+FFL   P  LRK  LF  LPL FR+ ++ YS  VK +A  +L +
Sbjct: 130 QAFVVSEDQKLDWADLFFLIMQPVQLRKRHLFPKLPLPFRDTLDMYSTRVKSIAKILLAK 189

Query: 196 FERALKITDGELTEMFTDG-NQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQINQVE 255
             +AL+I   E+ E+F D   Q+MRMNYYPPCP P  V G+ PHSDAVG TILLQ+N+V+
Sbjct: 190 MAKALQIKPEEVEEIFGDDMMQSMRMNYYPPCPQPNLVTGLIPHSDAVGLTILLQVNEVD 249

Query: 256 GLKIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIATFNNP 315
           GL+IKK+G W  V PL NAFIVN+GD++E+ITNG Y+SIEH A VN + ERLSIATF+N 
Sbjct: 250 GLQIKKNGKWFFVKPLQNAFIVNVGDVLEIITNGTYRSIEHRAMVNLEKERLSIATFHNT 309

Query: 316 RFDKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRI 355
             DKEI    SL+       F++L  +++++GLF+REL GK+YLD MRI
Sbjct: 310 GMDKEIGPARSLVQRQEAAKFRSLKTKDYLNGLFSRELKGKAYLDAMRI 358

BLAST of Sed0025336 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 357.5 bits (916), Expect = 1.3e-98
Identity = 173/347 (49.86%), Postives = 240/347 (69.16%), Query Frame = 0

Query: 16  SSILVPCVQELAKQP-IIEVPPRYLRHFQ-------IAASASEIPVIDMSKLLSDSATDS 75
           SS++VP V E+ K+     +PPRY+R  Q        ++ +SEIPVIDM++L S SA DS
Sbjct: 10  SSLIVPFVLEIVKEKNFTTIPPRYVRVDQEKTEILNDSSLSSEIPVIDMTRLCSVSAMDS 69

Query: 76  ELARLHSACENWGFFQLVKHGVRDSVIESMKIEAQRLFDLEMEEKKKLWQMEGDVEGFGQ 135
           EL +L  AC++WGFFQLV HG+  S +E ++ E Q  F+L M+EK+KLWQ  G+ EGFGQ
Sbjct: 70  ELKKLDFACQDWGFFQLVNHGIDSSFLEKLETEVQEFFNLPMKEKQKLWQRSGEFEGFGQ 129

Query: 136 AFVASEEQKLDWSDIFFLATAPAHLRKPRLFQNLPLSFRNAMEEYSAEVKDLAAAILRQF 195
             + SE QKLDW D+F L T P   RK  LF  LP  FR  +E YS+EVK +A  +  + 
Sbjct: 130 VNIVSENQKLDWGDMFILTTEPIRSRKSHLFSKLPPPFRETLETYSSEVKSIAKILFAKM 189

Query: 196 ERALKITDGELTEMFTDGNQTMRMNYYPPCPDPEKVIGVTPHSDAVGFTILLQINQVEGL 255
              L+I   E+ ++F D  Q++++NYYPPCP P++V+G+T HSDA G TILLQ+NQVEGL
Sbjct: 190 ASVLEIKHEEMEDLFDDVWQSIKINYYPPCPQPDQVMGLTQHSDAAGLTILLQVNQVEGL 249

Query: 256 KIKKDGNWIAVTPLPNAFIVNIGDLMEMITNGKYKSIEHCATVNSKSERLSIATFNNPRF 315
           +IKKDG W+ V PL +A +VN+G+++E+ITNG+Y+SIEH A VNS+ ERLS+A F++P  
Sbjct: 250 QIKKDGKWVVVKPLRDALVVNVGEILEIITNGRYRSIEHRAVVNSEKERLSVAMFHSPGK 309

Query: 316 DKEIKSPSSLITHHSPPLFKTLTYQEFVSGLFARELNGKSYLDTMRI 355
           +  I+   SL+      LFK+++ QE+    F ++LNGKS+LD MRI
Sbjct: 310 ETIIRPAKSLVDRQKQCLFKSMSTQEYFDAFFTQKLNGKSHLDLMRI 356