Sed0025313 (gene) Chayote v1

Overview
NameSed0025313
Typegene
OrganismSechium edule (Chayote v1)
DescriptionProtein transport protein Sec61 subunit alpha-like
LocationLG04: 37799556 .. 37803070 (+)
RNA-Seq ExpressionSed0025313
SyntenySed0025313
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGCGTGGCTTTCGAGTTCTTCACTTGGCGAGGCCGTTTCTTTCGTTCCTACCGGAAGCTCGAAGCCCTGATCGAAAAGTGCCATTCAGAGAAAAGGTCATTTATACTGTGATTTCACTTTTCATCTTCTTGGTTTGCAGCCAACTCCCTCTGTATGGCATACACTCCACAACTGGTGCAGATCCTTTTTATTGGTTGCGTGTGATTCTTGCTTTGAGTCGCGGCATTGTCATGGAACTTGGAATAACCCCCATTGTCACATCTGGGATGGTCATGCAGCTCTTAGCTGGGTCTAAGCTCATTCAAGTTGACAACAATGTCAGAGAGGATAGTGCCCTTTTGTAAGTCCGGTCTGTTCTTATGTTCTTTTCATTACCCTTCTTTATTTGATAATTACCATTGTGGGTTCGTTCTGTAAAAGGATAGCTTAAGTTGGTTAGTTCTGTTATGATTCATTTTTCACTACCAATTGTATAAATATGTCATCAATCAGATAAATAAAGAACTGAGAATTTCATTTACCAGAGTCCATGCACAATTGGGTATTCGGTCCTAAGAAAATGAAATTGGGATTCGATCTAATAAACCAAAGAGTATTGTCTTGAGGGATATGTTAAGGATCCCACATTCAAAAAGAGACGAGGCCTCAATAGGGTTTTTAAAAACTCCAATAGGCGCGCCTATGAGGCGTGGATAGAAAGGCAAAGTTTCAAATTCAATCAATGATTAATTCAAAGTTTTATATTAAAAAAATTAAAAACTAAAACCTAATCATCAATTTTCATCTTTGTTCTCTCTGATTATTTTCAATGAAGGAGTGGATGGGATGCATTTTTATGCATGGTGCACTTTGAAAAAAAAAAAAAAAAAAAAAAGCATGCACCATTTGTGCACCTTGCGCCTTAGGCTCCAAAGTGTCATTGCACATTTGTGTGCCTTTAAAAGCATCAGGCCTCACAACCTTTATAAGGTCATTGGCTACTTTCCGTGCCAATGGAACCATGTTATCTAATATTCTTTCCATTCTAATTGTAATTTTTCTCTAAATATGCTTGGTGATTGTGAGTTTTCTTTTCCTAAGATATAAGTAATTTGAATTTTATTGTTAATTCCCCATTTGATGATTCTTCTTTTATTCAATAGAAATGGAGCACAAAAGTTACTTGGTGTACTCATAGCAATTGGCATGGCTATGGCTTATGTTCTCTCTGCCATGTACGGCAGTATTGGTCAACTTGGCGTTGGAAATGCCATTCTTATCATTGTTCAGCTTTGTTTTGTTAGTATTATAGTAATTTGTTTGGATGAGCTTCTTCAGAAAGGATATGGCCTAGGCTCTGGAATTTCCCTCTTTATAGCTACTAATATCTGGTGAAATTCTTGACCTCCATTTCCTTCCCTTGCTCATGTATATTTGGCTTGACACTGTTTGTTCTACTCACTTTGTTTCTCAAGTATCAAAGCTTTTGATTTTTCTTTACTTTGCAGTGAGAGCATTATTTGGAAAGCATTTAGTCCCACTACAATCAACAGTGGACGAGGAGTTGAATTCGAAGGAGCTGTTATTGCTCTATTTCATCTGTTAATAGTTCGATCAGACAAAATTCGGGCACTTCGCGAGGAATTTTACCGACAAAATCTTCCCAATGTGACAAATTTTCTTTCAACCGTTTTCGTCTTTTTAATCGTCATCTACTTTCAAGGATTCTGGGTGGTTTTGCCTGTGAGGTCGAAGAATGCCCGCGGTCAACAAGGTTCATATCCGATCAAGCAGTTCTACACGTCCAACATGCCAATCATTCTGCAATCTACCCTTTTGTAAAATGTTTATTTCATCTCTCAGGTAGGTTGAAGACTCCCTATTCGAGTGTGATGTGTGCTTTTCTATGTAACTGATCATCTTGTAAATCTGCACAGCTGCTGTATAGGAAGTATGGTGGAAATTTTCTTGTGAATCTTTTGGGTATATGGAAAGTATCTGAGCATTCATCTGGTCAATCTGTTCTTGTGGGTGATCTTGCCTATTATATCACTGCACCTTCAAGGTCATTATTCCTTGTGAAATCAGTGTTCTTTTCTTTCTTTATTTTTCCAAGCAATGTAGTCAATTATTTCATTCTAACATTTGATAGTTGTCTTATCATTTCGCAGCTTAGCCAATATGGCAGCCAATCCTTTTCATGCCATGCTCTATCTGGTTTTTATGTTGTCTGCTTGCGCCTTCTCTCAAAATTATGGATTGAAGTATCAGGATCTTCTGCTAAAGATGTTGCTAAGCAGCTTAAGGTAGTCTTTCTAACTATGTCAGCCTCAGATGTTTTACTGCAACTTTAGGAAATCATTTGTCCAATGCTTGTCATTTTTTTTTTTATATCTGTGGCTGGCCGAATCAGCTTGTGTCCACCTTGACCAATGTTACTGGGAATACCTCATGACCCTACAACATTTGGATGTCAAGAAAACCCGTAGGAAATGAATTCTTAGGTAGGTAGCCACCATGAATTAAACTCGTCACCTTAAGCACCCCAAGTCCTTCGACCATTAGGCCACTATGATGGTTAATGCTTGCCATTTATAATGCCAAGACCTTTATTGTCAAGGTTACTGAAAATCTTTTCTTCCTAACAAGACAGTAAAACAGAGAAGCCTTTTGAACCTTATGTTAGAATTTCAGGAGATTATCGTTCATTGATAAACTGTAGGGTTTTAATTATGGCAAGTGCATTTGAACCATACAACGATAAACTCATACAAAATCTGTTGAGAAGAAACATGTTATTGAATAAATTGTATACCATAAAAACGAAATCATGGTATTATTTACTTCAAGTTGTTTTTGAGATGCATAAAAGTCAGAAAAGCACCCACCAAAATGGGTTTAGCCTAGCGGTAATTAGCATATATTCTCGACTAAGAGATTGCAGGCTCAAATTCTTCAAGCGTTCAAACGGTCTATTCTTGACCAAGATCTACCAATTTTATTATCAAAACACCAAGAAACAAGGAGTTCGAGATCTCCCACTCTCCAATCTCAAAGAGATGACCTAACACAATGAATTGGAATACCTTCTCCTAATCCCACCCAAACTACTTATACTAACCAACTTACAAAGACACTTCCCACACGCAACAACACGCACGTGCCCACACCCCCACACTCTATTTCTTACGCCTAGAATACACATATTTAACCGCCGGGGTTGTATCATTATGGAGATTGCTGAATCTTTTTTTCTATGTTGTGTTTACCTGTATTGTATATGATGATTATAGGAACAACAAATGGTGATGCCTTGACACAGAGAATCAAACCTACAGAAAGAGCTGAACCGCTACATACCAACAGCAGCAGCATTTGGAGGCATGTGCATTGGCGCACTCACTTGTTGGCTGATTTTATTGGCGCAATTGGTTCAGGGACAGGAGTTCTTCTTGCAGTCACAATCATTTACCAATACTTTGAAACTTTTGAGAAGGAAAAAGCCACCGAGCTTGGCTTCTTTGGCTTCTAG

mRNA sequence

ATGGGGCGTGGCTTTCGAGTTCTTCACTTGGCGAGGCCGTTTCTTTCGTTCCTACCGGAAGCTCGAAGCCCTGATCGAAAAGTGCCATTCAGAGAAAAGGTCATTTATACTGTGATTTCACTTTTCATCTTCTTGGTTTGCAGCCAACTCCCTCTGTATGGCATACACTCCACAACTGGTGCAGATCCTTTTTATTGGTTGCGTGTGATTCTTGCTTTGAGTCGCGGCATTGTCATGGAACTTGGAATAACCCCCATTGTCACATCTGGGATGGTCATGCAGCTCTTAGCTGGGTCTAAGCTCATTCAAGTTGACAACAATGTCAGAGAGGATAGTGCCCTTTTAAATGGAGCACAAAAGTTACTTGGTGTACTCATAGCAATTGGCATGGCTATGGCTTATGTTCTCTCTGCCATGTACGGCAGTATTGGTCAACTTGGCGTTGGAAATGCCATTCTTATCATTGTTCAGCTTTGTTTTGTTAGTATTATAGTAATTTGTTTGGATGAGCTTCTTCAGAAAGGATATGGCCTAGGCTCTGGAATTTCCCTCTTTATAGCTACTAATATCTGTGAGAGCATTATTTGGAAAGCATTTAGTCCCACTACAATCAACAGTGGACGAGGAGTTGAATTCGAAGGAGCTGTTATTGCTCTATTTCATCTGTTAATAGTTCGATCAGACAAAATTCGGGCACTTCGCGAGGAATTTTACCGACAAAATCTTCCCAATGTGACAAATTTTCTTTCAACCGTTTTCGTCTTTTTAATCGTCATCTACTTTCAAGGATTCTGGGTGGTTTTGCCTGTGAGGTCGAAGAATGCCCGCGGTCAACAAGGTTCATATCCGATCAAGCAGTTCTACACGTCCAACATGCCAATCATTCTGCAATCTACCCTTTTGAAGTATGGTGGAAATTTTCTTGTGAATCTTTTGGGTATATGGAAAGTATCTGAGCATTCATCTGGTCAATCTGTTCTTGTGGGTGATCTTGCCTATTATATCACTGCACCTTCAAGCTTAGCCAATATGGCAGCCAATCCTTTTCATGCCATGCTCTATCTGGTTTTTATGTTGTCTGCTTGCGCCTTCTCTCAAAATTATGGATTGAAGTATCAGGATCTTCTGCTAAAGATAGAATCAAACCTACAGAAAGAGCTGAACCGCTACATACCAACAGCAGCAGCATTTGGAGGCATGTGCATTGGCGCACTCACTTGGACAGGAGTTCTTCTTGCAGTCACAATCATTTACCAATACTTTGAAACTTTTGAGAAGGAAAAAGCCACCGAGCTTGGCTTCTTTGGCTTCTAG

Coding sequence (CDS)

ATGGGGCGTGGCTTTCGAGTTCTTCACTTGGCGAGGCCGTTTCTTTCGTTCCTACCGGAAGCTCGAAGCCCTGATCGAAAAGTGCCATTCAGAGAAAAGGTCATTTATACTGTGATTTCACTTTTCATCTTCTTGGTTTGCAGCCAACTCCCTCTGTATGGCATACACTCCACAACTGGTGCAGATCCTTTTTATTGGTTGCGTGTGATTCTTGCTTTGAGTCGCGGCATTGTCATGGAACTTGGAATAACCCCCATTGTCACATCTGGGATGGTCATGCAGCTCTTAGCTGGGTCTAAGCTCATTCAAGTTGACAACAATGTCAGAGAGGATAGTGCCCTTTTAAATGGAGCACAAAAGTTACTTGGTGTACTCATAGCAATTGGCATGGCTATGGCTTATGTTCTCTCTGCCATGTACGGCAGTATTGGTCAACTTGGCGTTGGAAATGCCATTCTTATCATTGTTCAGCTTTGTTTTGTTAGTATTATAGTAATTTGTTTGGATGAGCTTCTTCAGAAAGGATATGGCCTAGGCTCTGGAATTTCCCTCTTTATAGCTACTAATATCTGTGAGAGCATTATTTGGAAAGCATTTAGTCCCACTACAATCAACAGTGGACGAGGAGTTGAATTCGAAGGAGCTGTTATTGCTCTATTTCATCTGTTAATAGTTCGATCAGACAAAATTCGGGCACTTCGCGAGGAATTTTACCGACAAAATCTTCCCAATGTGACAAATTTTCTTTCAACCGTTTTCGTCTTTTTAATCGTCATCTACTTTCAAGGATTCTGGGTGGTTTTGCCTGTGAGGTCGAAGAATGCCCGCGGTCAACAAGGTTCATATCCGATCAAGCAGTTCTACACGTCCAACATGCCAATCATTCTGCAATCTACCCTTTTGAAGTATGGTGGAAATTTTCTTGTGAATCTTTTGGGTATATGGAAAGTATCTGAGCATTCATCTGGTCAATCTGTTCTTGTGGGTGATCTTGCCTATTATATCACTGCACCTTCAAGCTTAGCCAATATGGCAGCCAATCCTTTTCATGCCATGCTCTATCTGGTTTTTATGTTGTCTGCTTGCGCCTTCTCTCAAAATTATGGATTGAAGTATCAGGATCTTCTGCTAAAGATAGAATCAAACCTACAGAAAGAGCTGAACCGCTACATACCAACAGCAGCAGCATTTGGAGGCATGTGCATTGGCGCACTCACTTGGACAGGAGTTCTTCTTGCAGTCACAATCATTTACCAATACTTTGAAACTTTTGAGAAGGAAAAAGCCACCGAGCTTGGCTTCTTTGGCTTCTAG

Protein sequence

MGRGFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADPFYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQKLLGVLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGSGISLFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQNLPNVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTLLKYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANPFHAMLYLVFMLSACAFSQNYGLKYQDLLLKIESNLQKELNRYIPTAAAFGGMCIGALTWTGVLLAVTIIYQYFETFEKEKATELGFFGF
Homology
BLAST of Sed0025313 vs. NCBI nr
Match: XP_038897181.1 (protein transport protein Sec61 subunit alpha-like [Benincasa hispida])

HSP 1 Score: 714.5 bits (1843), Expect = 5.5e-202
Identity = 383/476 (80.46%), Postives = 409/476 (85.92%), Query Frame = 0

Query: 1   MGRGFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MG GFRVLHL RPFLSFLPE ++ DRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLSFLPEVQNADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQK 120
           ADPFYW+RVILA +RG VMELGITPIVTSGMVMQLLAGSK+I+VDNNVRED ALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLAGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGVLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGS 180
           LLG+LIAIG A+AYVLS MYGS+GQLGVGNAILIIVQLCF  IIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVGQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQ 240
           GISLFIATNICE+IIWKAFSPTTINSGRGVEFEGAVIALFHLLI+RSDKIRALRE FYRQ
Sbjct: 181 GISLFIATNICENIIWKAFSPTTINSGRGVEFEGAVIALFHLLIIRSDKIRALREAFYRQ 240

Query: 241 NLPNVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL 300
           NLPNVTN L+TVF+FLIVIYFQGF VVLPVRSKNARGQQGSYPIK FYTSNMPIILQS L
Sbjct: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 L------------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANP 360
           +            KY GNFLVNLLGIWK SE+S+GQSV VG LAYYITAPSSLA+MAANP
Sbjct: 301 VSNVYFISQLLYRKYSGNFLVNLLGIWKESEYSAGQSVPVGGLAYYITAPSSLADMAANP 360

Query: 361 FHAMLYLVFMLSACA-FSQNY-------------GLKYQDLLL--KIESNLQKELNRYIP 420
           +HA+ YLVFMLSACA FS+ +              LK Q +++    ESNLQKELNRYIP
Sbjct: 361 YHAVFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFGF 438
           TAAAFGGMCIGALT            TG+LLAVTIIYQYFETFEKEKA+ELGFFGF
Sbjct: 421 TAAAFGGMCIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGFFGF 476

BLAST of Sed0025313 vs. NCBI nr
Match: XP_008463755.1 (PREDICTED: protein transport protein Sec61 subunit alpha-like [Cucumis melo] >XP_008463756.1 PREDICTED: protein transport protein Sec61 subunit alpha-like [Cucumis melo] >KAA0066757.1 protein transport protein Sec61 subunit alpha-like [Cucumis melo var. makuwa] >TYK27904.1 protein transport protein Sec61 subunit alpha-like [Cucumis melo var. makuwa])

HSP 1 Score: 709.9 bits (1831), Expect = 1.3e-200
Identity = 378/476 (79.41%), Postives = 406/476 (85.29%), Query Frame = 0

Query: 1   MGRGFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MG GFRV+HLARPFLSFLPE ++ DRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVIHLARPFLSFLPEVQNADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQK 120
           ADPFYW+RVILA +RG VMELGITPIVTSGMVMQLL GSK+I+VDNNVRED  LLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRTLLNGAQK 120

Query: 121 LLGVLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGS 180
           LLG+LIAIG A+AYVLS MYGS+GQLG GNAILII+QLCF  IIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVGQLGAGNAILIIIQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQ 240
           GISLFIATNICE+IIWKAFSPTTINSGRG+EFEGAVIALFHLLI+RSDKIRALRE FYRQ
Sbjct: 181 GISLFIATNICENIIWKAFSPTTINSGRGIEFEGAVIALFHLLIIRSDKIRALREAFYRQ 240

Query: 241 NLPNVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL 300
           NLPNVTN L+TVF+FLIVIYFQGF VVLPVRSKNARGQQGSYPIK FYTSNMPIILQS L
Sbjct: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 L------------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANP 360
           +            KY GNF+VNLLGIWK SE+S+GQSV VG LAYYITAPSSLA+MAANP
Sbjct: 301 VSNVYFISQLLYRKYSGNFIVNLLGIWKESEYSAGQSVPVGGLAYYITAPSSLADMAANP 360

Query: 361 FHAMLYLVFMLSACA-FSQNY-------------GLKYQDLLL--KIESNLQKELNRYIP 420
           FHAM YLVFMLSACA FS+ +              LK Q +++    ESNLQKELNRYIP
Sbjct: 361 FHAMFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFGF 438
           TAAAFGG+CIGALT            TG+LLAVTIIYQYFETFEKEKA ELGFFGF
Sbjct: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKANELGFFGF 476

BLAST of Sed0025313 vs. NCBI nr
Match: XP_030550124.1 (protein transport protein Sec61 subunit alpha-like [Rhodamnia argentea])

HSP 1 Score: 707.2 bits (1824), Expect = 8.7e-200
Identity = 376/476 (78.99%), Postives = 406/476 (85.29%), Query Frame = 0

Query: 1   MGRGFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MG GFRVLHL RPFLSFLPE +S DRK+PFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKIPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQK 120
           ADPFYW+RVILA +RG VMELGITPIVTSG+VMQLLAGSK+I+VDNNVRED ALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGLVMQLLAGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGVLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGS 180
           LLG+LIAIG A+AYVLS MYGS+GQLGVGNAILII+QLCF  IIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVGQLGVGNAILIIIQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQ 240
           GISLFIATNICE+IIWKAFSPTTINSGRG EFEGAVIALFHLLI R+DK+RALRE FYRQ
Sbjct: 181 GISLFIATNICETIIWKAFSPTTINSGRGAEFEGAVIALFHLLITRTDKVRALREAFYRQ 240

Query: 241 NLPNVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL 300
           NLPNVTN L+T+ VFLIVIYFQGF VVLPVRSKNARGQQGSYPIK FYTSNMPIILQS L
Sbjct: 241 NLPNVTNLLATILVFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 L------------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANP 360
           +            KY GNFLVNLLGIWK SE+SSGQSV VG +AYYITAPSSLA+MAANP
Sbjct: 301 VSNIYFISQLLYRKYSGNFLVNLLGIWKESEYSSGQSVPVGGIAYYITAPSSLADMAANP 360

Query: 361 FHAMLYLVFMLSACA-FSQNY----GLKYQDLLLKI-----------ESNLQKELNRYIP 420
           FHA+ YLVFMLSACA FS+ +    G   +D+  ++           ESNLQKELNRYIP
Sbjct: 361 FHALFYLVFMLSACALFSKTWIEVSGSSAKDVARQLREQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFGF 438
           TAAAFGG+CIGALT            TG+LLAVTIIYQYFETFEKEKA+ELGFFGF
Sbjct: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGFFGF 476

BLAST of Sed0025313 vs. NCBI nr
Match: XP_022140644.1 (protein transport protein Sec61 subunit alpha-like [Momordica charantia])

HSP 1 Score: 706.4 bits (1822), Expect = 1.5e-199
Identity = 379/476 (79.62%), Postives = 404/476 (84.87%), Query Frame = 0

Query: 1   MGRGFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MG GFRVLHL RPFLSFLPE +S DRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQK 120
           ADPFYW+RVILA +RG VMELGITPIVTSGMVMQLL GSK+I+VDNNVRED ALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGVLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGS 180
           LLG+LIAIG A+AYVLS MYGS+ QLGVGNAILIIVQLCF  IIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQ 240
           GISLFIATNICE+IIWKAFSPTTINSGRG EFEGAVIALFHLLI RSDK+RALRE FYRQ
Sbjct: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREPFYRQ 240

Query: 241 NLPNVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL 300
           NLPNVTN L+TVF+FLIVIY QGF VVLPVRSKNARGQQGSYPIK FYTSNMPIILQS L
Sbjct: 241 NLPNVTNLLATVFIFLIVIYVQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 L------------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANP 360
           +            KYGGNFLVNLLGIWK SE+SSGQSV VG LAYYITAPSSLA+MAANP
Sbjct: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360

Query: 361 FHAMLYLVFMLSACA-FSQNY-------------GLKYQDLLL--KIESNLQKELNRYIP 420
           FHA+ YL+FMLSACA FS+ +              LK Q +++    ESNLQKELNRYIP
Sbjct: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFGF 438
           TAAAFGG+CIGALT            TG+LLAVTIIYQYFETFEKEKA+ELG FGF
Sbjct: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 476

BLAST of Sed0025313 vs. NCBI nr
Match: XP_004146019.1 (protein transport protein Sec61 subunit alpha [Cucumis sativus] >KGN55053.1 hypothetical protein Csa_012650 [Cucumis sativus])

HSP 1 Score: 705.7 bits (1820), Expect = 2.5e-199
Identity = 374/476 (78.57%), Postives = 406/476 (85.29%), Query Frame = 0

Query: 1   MGRGFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MG GFRVLHLARPFLSFLPE ++ DRKVPFREKVIYTVISLF+FLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLARPFLSFLPEVQNADRKVPFREKVIYTVISLFVFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQK 120
           ADPFYW+RVILA +RG VMELGITPIVTSGMVMQLL GSK+I++DNNVRED ALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEIDNNVREDRALLNGAQK 120

Query: 121 LLGVLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGS 180
           LLG+LIAIG A+AYVLS MYGS+GQLG GNAILII+QLCF  IIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVGQLGAGNAILIIIQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQ 240
           GISLFIATNICE+IIWKAFSPTTINSGRG+EFEGAVIALFHLLI+RSDKI+ALRE FYRQ
Sbjct: 181 GISLFIATNICENIIWKAFSPTTINSGRGIEFEGAVIALFHLLIIRSDKIQALREAFYRQ 240

Query: 241 NLPNVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL 300
           NLPNVTN L+TVF+FLIVIYFQGF VVLPVRSKNARGQQGSYPIK FYTSNMPIILQS L
Sbjct: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 L------------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANP 360
           +            K+ GNF+VNLLGIWK SE+S+GQSV VG LAYYITAPSS A+MAANP
Sbjct: 301 VSNVYFISQLLYRKFSGNFIVNLLGIWKESEYSAGQSVPVGGLAYYITAPSSFADMAANP 360

Query: 361 FHAMLYLVFMLSACA-FSQNY-------------GLKYQDLLL--KIESNLQKELNRYIP 420
           FHAM YLVFMLSACA FS+ +              LK Q +++    ESNLQKELNRYIP
Sbjct: 361 FHAMFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFGF 438
           TAAAFGG+CIGALT            TG+LLAVTIIYQYFETFEKEK+ ELGFFGF
Sbjct: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKSNELGFFGF 476

BLAST of Sed0025313 vs. ExPASy Swiss-Prot
Match: Q54XK2 (Protein transport protein Sec61 subunit alpha OS=Dictyostelium discoideum OX=44689 GN=sec61a PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.2e-146
Identity = 273/472 (57.84%), Postives = 346/472 (73.31%), Query Frame = 0

Query: 4   GFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADP 63
           GFR L + +PF S +PE   PDRK+PFREKV++T I LFIFLVCSQ+PLYGI ST  +DP
Sbjct: 2   GFRFLDIVKPFTSLVPEVGQPDRKIPFREKVLWTAICLFIFLVCSQIPLYGIRSTDSSDP 61

Query: 64  FYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQKLLG 123
           FYW +VI+A +RG +MELGI+PIVTSGMVMQLLAG+KLI++D +V+ D  L + AQKL G
Sbjct: 62  FYWAKVIMASNRGTLMELGISPIVTSGMVMQLLAGAKLIEIDQSVKADRDLFSAAQKLFG 121

Query: 124 VLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGSGIS 183
           +LI +G  +AY+ S  YG    LG GN  LI++QL F  IIV+ LDELLQKGYG+GSGIS
Sbjct: 122 MLICVGQGVAYIWSGSYGDPAVLGFGNCFLIVLQLFFAGIIVMLLDELLQKGYGIGSGIS 181

Query: 184 LFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQNLP 243
           LFIATNICE+I+WK FSPTT++ G+G EFEGAVIALFHLL+ R+DK+RAL+E FYRQNLP
Sbjct: 182 LFIATNICETIVWKTFSPTTVSVGKGTEFEGAVIALFHLLLTRNDKVRALKEAFYRQNLP 241

Query: 244 NVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTLL-- 303
           N+TN L+TV +F++VIYFQGF V LPV+S    GQQG+YPIK FYTSN+PIILQS L+  
Sbjct: 242 NITNLLATVLIFMVVIYFQGFRVDLPVKSTRVSGQQGTYPIKLFYTSNIPIILQSALVSN 301

Query: 304 ----------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANPFHA 363
                     ++  N LVNL G W+ SE+S  Q + V  L YYI++P++++ + A+PFHA
Sbjct: 302 LYFISQLLYRRFPDNILVNLFGAWRTSEYSQ-QMIPVSGLTYYISSPNNMSAVLADPFHA 361

Query: 364 MLYLVFMLSACA-FSQNY-------------GLKYQDLLLK--IESNLQKELNRYIPTAA 423
           + Y+ FML++CA FS+ +              LK Q + +K   ++++ KELNRYIPTAA
Sbjct: 362 LFYITFMLTSCALFSKVWIEVSGSSARDVAKQLKDQQMTMKGHRDTSVIKELNRYIPTAA 421

Query: 424 AFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFG 437
           AFGG+CIGALT            TG+LLAVTIIYQYFETF KE+    G  G
Sbjct: 422 AFGGLCIGALTVVADFMGAIGSGTGILLAVTIIYQYFETFVKEQQELSGGIG 472

BLAST of Sed0025313 vs. ExPASy Swiss-Prot
Match: Q2KHX4 (Protein transport protein Sec61 subunit alpha isoform 2 OS=Bos taurus OX=9913 GN=SEC61A2 PE=2 SV=3)

HSP 1 Score: 501.5 bits (1290), Expect = 9.5e-141
Identity = 268/473 (56.66%), Postives = 342/473 (72.30%), Query Frame = 0

Query: 4   GFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADP 63
           G + L + +PF + LPE + P+RK+ FREKV++T I+LFIFLVC Q+PL+GI S+  ADP
Sbjct: 2   GIKFLEVIKPFCAVLPEIQKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 61

Query: 64  FYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQKLLG 123
           FYW+RVILA +RG +MELGI+PIVTSG++MQLLAG+K+I+V  +  +D AL NGAQKL G
Sbjct: 62  FYWMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEV-GDTPKDRALFNGAQKLFG 121

Query: 124 VLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGSGIS 183
           ++I IG A+ YV++ MYG   ++G G  +LII+QL    +IV+ LDELLQKGYGLGSGIS
Sbjct: 122 MIITIGQAIVYVMTGMYGDPAEMGAGICLLIIIQLFVAGLIVLLLDELLQKGYGLGSGIS 181

Query: 184 LFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQNLP 243
           LFIATNICE+I+WKAFSPTTIN+GRG EFEGAVIALFHLL  R+DK+RALRE FYRQNLP
Sbjct: 182 LFIATNICETIVWKAFSPTTINTGRGTEFEGAVIALFHLLATRTDKVRALREAFYRQNLP 241

Query: 244 NVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL--- 303
           N+ N ++TVFVF +VIYFQGF V LP++S   RGQ  SYPIK FYTSN+PIILQS L   
Sbjct: 242 NLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSN 301

Query: 304 ---------LKYGGNFLVNLLGIW-KVSEHSSGQSVLVGDLAYYITAPSSLANMAANPFH 363
                    +++ GNFLVNLLG W  VS     +S  VG L YY++ P S+  +  +P H
Sbjct: 302 LYVISQMLSVRFSGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFEDPVH 361

Query: 364 AMLYLVFMLSACAFSQN--------------YGLKYQDLLLK--IESNLQKELNRYIPTA 423
            ++Y++FML +CAF                   LK Q ++++   ++++  ELNRYIPTA
Sbjct: 362 VVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNRYIPTA 421

Query: 424 AAFGGMCIGAL-----------TWTGVLLAVTIIYQYFETFEKEKATELGFFG 437
           AAFGG+CIGAL           + TG+LLAVTIIYQYFE F KE+A E+G  G
Sbjct: 422 AAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQA-EVGGMG 472

BLAST of Sed0025313 vs. ExPASy Swiss-Prot
Match: Q9H9S3 (Protein transport protein Sec61 subunit alpha isoform 2 OS=Homo sapiens OX=9606 GN=SEC61A2 PE=2 SV=3)

HSP 1 Score: 501.5 bits (1290), Expect = 9.5e-141
Identity = 268/473 (56.66%), Postives = 342/473 (72.30%), Query Frame = 0

Query: 4   GFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADP 63
           G + L + +PF + LPE + P+RK+ FREKV++T I+LFIFLVC Q+PL+GI S+  ADP
Sbjct: 2   GIKFLEVIKPFCAVLPEIQKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 61

Query: 64  FYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQKLLG 123
           FYW+RVILA +RG +MELGI+PIVTSG++MQLLAG+K+I+V  +  +D AL NGAQKL G
Sbjct: 62  FYWMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEV-GDTPKDRALFNGAQKLFG 121

Query: 124 VLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGSGIS 183
           ++I IG A+ YV++ MYG   ++G G  +LII+QL    +IV+ LDELLQKGYGLGSGIS
Sbjct: 122 MIITIGQAIVYVMTGMYGDPAEMGAGICLLIIIQLFVAGLIVLLLDELLQKGYGLGSGIS 181

Query: 184 LFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQNLP 243
           LFIATNICE+I+WKAFSPTTIN+GRG EFEGAVIALFHLL  R+DK+RALRE FYRQNLP
Sbjct: 182 LFIATNICETIVWKAFSPTTINTGRGTEFEGAVIALFHLLATRTDKVRALREAFYRQNLP 241

Query: 244 NVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL--- 303
           N+ N ++TVFVF +VIYFQGF V LP++S   RGQ  SYPIK FYTSN+PIILQS L   
Sbjct: 242 NLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSN 301

Query: 304 ---------LKYGGNFLVNLLGIW-KVSEHSSGQSVLVGDLAYYITAPSSLANMAANPFH 363
                    +++ GNFLVNLLG W  VS     +S  VG L YY++ P S+  +  +P H
Sbjct: 302 LYVISQMLSVRFSGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFEDPVH 361

Query: 364 AMLYLVFMLSACAFSQN--------------YGLKYQDLLLK--IESNLQKELNRYIPTA 423
            ++Y++FML +CAF                   LK Q ++++   ++++  ELNRYIPTA
Sbjct: 362 VVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNRYIPTA 421

Query: 424 AAFGGMCIGAL-----------TWTGVLLAVTIIYQYFETFEKEKATELGFFG 437
           AAFGG+CIGAL           + TG+LLAVTIIYQYFE F KE+A E+G  G
Sbjct: 422 AAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQA-EVGGMG 472

BLAST of Sed0025313 vs. ExPASy Swiss-Prot
Match: Q9JLR1 (Protein transport protein Sec61 subunit alpha isoform 2 OS=Mus musculus OX=10090 GN=Sec61a2 PE=2 SV=3)

HSP 1 Score: 501.5 bits (1290), Expect = 9.5e-141
Identity = 268/473 (56.66%), Postives = 342/473 (72.30%), Query Frame = 0

Query: 4   GFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADP 63
           G + L + +PF + LPE + P+RK+ FREKV++T I+LFIFLVC Q+PL+GI S+  ADP
Sbjct: 2   GIKFLEVIKPFCAVLPEIQKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 61

Query: 64  FYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQKLLG 123
           FYW+RVILA +RG +MELGI+PIVTSG++MQLLAG+K+I+V  +  +D AL NGAQKL G
Sbjct: 62  FYWMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEV-GDTPKDRALFNGAQKLFG 121

Query: 124 VLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGSGIS 183
           ++I IG A+ YV++ MYG   ++G G  +LII+QL    +IV+ LDELLQKGYGLGSGIS
Sbjct: 122 MIITIGQAIVYVMTGMYGDPAEMGAGICLLIIIQLFVAGLIVLLLDELLQKGYGLGSGIS 181

Query: 184 LFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQNLP 243
           LFIATNICE+I+WKAFSPTTIN+GRG EFEGAVIALFHLL  R+DK+RALRE FYRQNLP
Sbjct: 182 LFIATNICETIVWKAFSPTTINTGRGTEFEGAVIALFHLLATRTDKVRALREAFYRQNLP 241

Query: 244 NVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL--- 303
           N+ N ++TVFVF +VIYFQGF V LP++S   RGQ  SYPIK FYTSN+PIILQS L   
Sbjct: 242 NLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSN 301

Query: 304 ---------LKYGGNFLVNLLGIW-KVSEHSSGQSVLVGDLAYYITAPSSLANMAANPFH 363
                    +++ GNFLVNLLG W  VS     +S  VG L YY++ P S+  +  +P H
Sbjct: 302 LYVISQMLSVRFSGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFEDPVH 361

Query: 364 AMLYLVFMLSACAFSQN--------------YGLKYQDLLLK--IESNLQKELNRYIPTA 423
            ++Y++FML +CAF                   LK Q ++++   ++++  ELNRYIPTA
Sbjct: 362 VVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNRYIPTA 421

Query: 424 AAFGGMCIGAL-----------TWTGVLLAVTIIYQYFETFEKEKATELGFFG 437
           AAFGG+CIGAL           + TG+LLAVTIIYQYFE F KE+A E+G  G
Sbjct: 422 AAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQA-EVGGMG 472

BLAST of Sed0025313 vs. ExPASy Swiss-Prot
Match: Q5NVM7 (Protein transport protein Sec61 subunit alpha isoform 2 OS=Pongo abelii OX=9601 GN=SEC61A2 PE=2 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 6.2e-140
Identity = 267/473 (56.45%), Postives = 341/473 (72.09%), Query Frame = 0

Query: 4   GFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADP 63
           G + L + +PF + LPE + P+RK+ FREKV++T I+LFIFLVC Q+PL+GI S+  ADP
Sbjct: 2   GIKFLEVIKPFCAVLPEIQKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 61

Query: 64  FYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQKLLG 123
           FYW+RVILA +RG +MELGI+PIVTSG++MQLLAG+K+I+V  +  +D AL NGAQKL G
Sbjct: 62  FYWMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEV-GDTPKDRALFNGAQKLFG 121

Query: 124 VLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGSGIS 183
           ++I IG A+ YV++ MYG   ++G G  +LII+QL    +IV+ LDELLQKGYGLGSGIS
Sbjct: 122 MIITIGQAIVYVMTGMYGDPAEMGAGICLLIIIQLFVAGLIVLLLDELLQKGYGLGSGIS 181

Query: 184 LFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQNLP 243
           LFIATNICE+I+WKA SPTTIN+GRG EFEGAVIALFHLL  R+DK+RALRE FYRQNLP
Sbjct: 182 LFIATNICETIVWKASSPTTINTGRGTEFEGAVIALFHLLATRTDKVRALREAFYRQNLP 241

Query: 244 NVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL--- 303
           N+ N ++TVFVF +VIYFQGF V LP++S   RGQ  SYPIK FYTSN+PIILQS L   
Sbjct: 242 NLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSN 301

Query: 304 ---------LKYGGNFLVNLLGIW-KVSEHSSGQSVLVGDLAYYITAPSSLANMAANPFH 363
                    +++ GNFLVNLLG W  VS     +S  VG L YY++ P S+  +  +P H
Sbjct: 302 LYVISQMLSVRFSGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAILEDPVH 361

Query: 364 AMLYLVFMLSACAFSQN--------------YGLKYQDLLLK--IESNLQKELNRYIPTA 423
            ++Y++FML +CAF                   LK Q ++++   ++++  ELNRYIPTA
Sbjct: 362 VVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNRYIPTA 421

Query: 424 AAFGGMCIGAL-----------TWTGVLLAVTIIYQYFETFEKEKATELGFFG 437
           AAFGG+CIGAL           + TG+LLAVTIIYQYFE F KE+A E+G  G
Sbjct: 422 AAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQA-EVGGMG 472

BLAST of Sed0025313 vs. ExPASy TrEMBL
Match: A0A1S3CK18 (protein transport protein Sec61 subunit alpha-like OS=Cucumis melo OX=3656 GN=LOC103501823 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 6.5e-201
Identity = 378/476 (79.41%), Postives = 406/476 (85.29%), Query Frame = 0

Query: 1   MGRGFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MG GFRV+HLARPFLSFLPE ++ DRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVIHLARPFLSFLPEVQNADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQK 120
           ADPFYW+RVILA +RG VMELGITPIVTSGMVMQLL GSK+I+VDNNVRED  LLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRTLLNGAQK 120

Query: 121 LLGVLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGS 180
           LLG+LIAIG A+AYVLS MYGS+GQLG GNAILII+QLCF  IIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVGQLGAGNAILIIIQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQ 240
           GISLFIATNICE+IIWKAFSPTTINSGRG+EFEGAVIALFHLLI+RSDKIRALRE FYRQ
Sbjct: 181 GISLFIATNICENIIWKAFSPTTINSGRGIEFEGAVIALFHLLIIRSDKIRALREAFYRQ 240

Query: 241 NLPNVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL 300
           NLPNVTN L+TVF+FLIVIYFQGF VVLPVRSKNARGQQGSYPIK FYTSNMPIILQS L
Sbjct: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 L------------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANP 360
           +            KY GNF+VNLLGIWK SE+S+GQSV VG LAYYITAPSSLA+MAANP
Sbjct: 301 VSNVYFISQLLYRKYSGNFIVNLLGIWKESEYSAGQSVPVGGLAYYITAPSSLADMAANP 360

Query: 361 FHAMLYLVFMLSACA-FSQNY-------------GLKYQDLLL--KIESNLQKELNRYIP 420
           FHAM YLVFMLSACA FS+ +              LK Q +++    ESNLQKELNRYIP
Sbjct: 361 FHAMFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFGF 438
           TAAAFGG+CIGALT            TG+LLAVTIIYQYFETFEKEKA ELGFFGF
Sbjct: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKANELGFFGF 476

BLAST of Sed0025313 vs. ExPASy TrEMBL
Match: A0A5A7VI03 (Protein transport protein Sec61 subunit alpha-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G00890 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 6.5e-201
Identity = 378/476 (79.41%), Postives = 406/476 (85.29%), Query Frame = 0

Query: 1   MGRGFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MG GFRV+HLARPFLSFLPE ++ DRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVIHLARPFLSFLPEVQNADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQK 120
           ADPFYW+RVILA +RG VMELGITPIVTSGMVMQLL GSK+I+VDNNVRED  LLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRTLLNGAQK 120

Query: 121 LLGVLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGS 180
           LLG+LIAIG A+AYVLS MYGS+GQLG GNAILII+QLCF  IIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVGQLGAGNAILIIIQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQ 240
           GISLFIATNICE+IIWKAFSPTTINSGRG+EFEGAVIALFHLLI+RSDKIRALRE FYRQ
Sbjct: 181 GISLFIATNICENIIWKAFSPTTINSGRGIEFEGAVIALFHLLIIRSDKIRALREAFYRQ 240

Query: 241 NLPNVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL 300
           NLPNVTN L+TVF+FLIVIYFQGF VVLPVRSKNARGQQGSYPIK FYTSNMPIILQS L
Sbjct: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 L------------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANP 360
           +            KY GNF+VNLLGIWK SE+S+GQSV VG LAYYITAPSSLA+MAANP
Sbjct: 301 VSNVYFISQLLYRKYSGNFIVNLLGIWKESEYSAGQSVPVGGLAYYITAPSSLADMAANP 360

Query: 361 FHAMLYLVFMLSACA-FSQNY-------------GLKYQDLLL--KIESNLQKELNRYIP 420
           FHAM YLVFMLSACA FS+ +              LK Q +++    ESNLQKELNRYIP
Sbjct: 361 FHAMFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFGF 438
           TAAAFGG+CIGALT            TG+LLAVTIIYQYFETFEKEKA ELGFFGF
Sbjct: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKANELGFFGF 476

BLAST of Sed0025313 vs. ExPASy TrEMBL
Match: A0A6J1CG91 (protein transport protein Sec61 subunit alpha-like OS=Momordica charantia OX=3673 GN=LOC111011251 PE=3 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 7.2e-200
Identity = 379/476 (79.62%), Postives = 404/476 (84.87%), Query Frame = 0

Query: 1   MGRGFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MG GFRVLHL RPFLSFLPE +S DRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQK 120
           ADPFYW+RVILA +RG VMELGITPIVTSGMVMQLL GSK+I+VDNNVRED ALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGVLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGS 180
           LLG+LIAIG A+AYVLS MYGS+ QLGVGNAILIIVQLCF  IIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQ 240
           GISLFIATNICE+IIWKAFSPTTINSGRG EFEGAVIALFHLLI RSDK+RALRE FYRQ
Sbjct: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREPFYRQ 240

Query: 241 NLPNVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL 300
           NLPNVTN L+TVF+FLIVIY QGF VVLPVRSKNARGQQGSYPIK FYTSNMPIILQS L
Sbjct: 241 NLPNVTNLLATVFIFLIVIYVQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 L------------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANP 360
           +            KYGGNFLVNLLGIWK SE+SSGQSV VG LAYYITAPSSLA+MAANP
Sbjct: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360

Query: 361 FHAMLYLVFMLSACA-FSQNY-------------GLKYQDLLL--KIESNLQKELNRYIP 420
           FHA+ YL+FMLSACA FS+ +              LK Q +++    ESNLQKELNRYIP
Sbjct: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFGF 438
           TAAAFGG+CIGALT            TG+LLAVTIIYQYFETFEKEKA+ELG FGF
Sbjct: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 476

BLAST of Sed0025313 vs. ExPASy TrEMBL
Match: A0A0A0L016 (Plug_translocon domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G625570 PE=3 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 1.2e-199
Identity = 374/476 (78.57%), Postives = 406/476 (85.29%), Query Frame = 0

Query: 1   MGRGFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MG GFRVLHLARPFLSFLPE ++ DRKVPFREKVIYTVISLF+FLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLARPFLSFLPEVQNADRKVPFREKVIYTVISLFVFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQK 120
           ADPFYW+RVILA +RG VMELGITPIVTSGMVMQLL GSK+I++DNNVRED ALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEIDNNVREDRALLNGAQK 120

Query: 121 LLGVLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGS 180
           LLG+LIAIG A+AYVLS MYGS+GQLG GNAILII+QLCF  IIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVGQLGAGNAILIIIQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQ 240
           GISLFIATNICE+IIWKAFSPTTINSGRG+EFEGAVIALFHLLI+RSDKI+ALRE FYRQ
Sbjct: 181 GISLFIATNICENIIWKAFSPTTINSGRGIEFEGAVIALFHLLIIRSDKIQALREAFYRQ 240

Query: 241 NLPNVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL 300
           NLPNVTN L+TVF+FLIVIYFQGF VVLPVRSKNARGQQGSYPIK FYTSNMPIILQS L
Sbjct: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 L------------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANP 360
           +            K+ GNF+VNLLGIWK SE+S+GQSV VG LAYYITAPSS A+MAANP
Sbjct: 301 VSNVYFISQLLYRKFSGNFIVNLLGIWKESEYSAGQSVPVGGLAYYITAPSSFADMAANP 360

Query: 361 FHAMLYLVFMLSACA-FSQNY-------------GLKYQDLLL--KIESNLQKELNRYIP 420
           FHAM YLVFMLSACA FS+ +              LK Q +++    ESNLQKELNRYIP
Sbjct: 361 FHAMFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFGF 438
           TAAAFGG+CIGALT            TG+LLAVTIIYQYFETFEKEK+ ELGFFGF
Sbjct: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKSNELGFFGF 476

BLAST of Sed0025313 vs. ExPASy TrEMBL
Match: A0A059BMT1 (Plug_translocon domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_F01251 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 2.1e-199
Identity = 375/476 (78.78%), Postives = 404/476 (84.87%), Query Frame = 0

Query: 1   MGRGFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MG GFRVLHL RPFLSFLPE +S DRK+PFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKIPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQK 120
           ADPFYW+RVILA +RG VMELGITPIVTSG+VMQLLAGSK+I+VDNNVRED ALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGLVMQLLAGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGVLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGS 180
           LLG+LIAIG A+AYVLS MYGS+GQLGVGNAILII+QLCF  IIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVGQLGVGNAILIIIQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQ 240
           GISLFIATNICESIIWKAFSPTTINSGRG EFEGAVIALFHLLI R+DK+RALRE FYRQ
Sbjct: 181 GISLFIATNICESIIWKAFSPTTINSGRGAEFEGAVIALFHLLITRTDKVRALREAFYRQ 240

Query: 241 NLPNVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL 300
           NLPNVTN L+TV +FLIVIYFQGF VVLPVRSKNARGQQGSYPIK FYTSNMPIILQS L
Sbjct: 241 NLPNVTNLLATVLIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 L------------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANP 360
           +            KY GNF+VNLLG WK SE+S GQSV VG +AYYITAPSSLA+MAANP
Sbjct: 301 VTNIYFISQLLHRKYSGNFIVNLLGKWKESEYSGGQSVPVGGIAYYITAPSSLADMAANP 360

Query: 361 FHAMLYLVFMLSACA-FSQNY----GLKYQDLLLKI-----------ESNLQKELNRYIP 420
           FHA+ YLVFMLSACA FS+ +    G   +D+  ++           ESNLQKELNRYIP
Sbjct: 361 FHALFYLVFMLSACALFSKTWIEVSGSSAKDVARQLREQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFGF 438
           TAAAFGGMCIGALT            TG+LLAVTIIYQYFETFEKEKA+ELGFFGF
Sbjct: 421 TAAAFGGMCIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGFFGF 476

BLAST of Sed0025313 vs. TAIR 10
Match: AT1G78720.1 (SecY protein transport family protein )

HSP 1 Score: 682.6 bits (1760), Expect = 2.1e-196
Identity = 359/472 (76.06%), Postives = 401/472 (84.96%), Query Frame = 0

Query: 4   GFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADP 63
           GFRV+HL RPFL+FLPE +SP+RK+PFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADP
Sbjct: 4   GFRVIHLVRPFLAFLPEVQSPERKIPFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADP 63

Query: 64  FYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQKLLG 123
           FYW+RVILA SRG VMELGITPIVTSGMVMQLLAGSK+I++DNNVRED ALLNGAQKLLG
Sbjct: 64  FYWMRVILASSRGTVMELGITPIVTSGMVMQLLAGSKIIEIDNNVREDRALLNGAQKLLG 123

Query: 124 VLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGSGIS 183
           +LIA+G A+AYVLS MYGS+G+LGVGNAILIIVQLCF +IIV+CLDELLQKGYGLGSGIS
Sbjct: 124 ILIAVGQAVAYVLSGMYGSVGELGVGNAILIIVQLCFAAIIVLCLDELLQKGYGLGSGIS 183

Query: 184 LFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQNLP 243
           LFIATNICESIIWKAFSPTTINSGRG +FEGAVIALFHLLI R+DK+RALRE F+RQNLP
Sbjct: 184 LFIATNICESIIWKAFSPTTINSGRGAQFEGAVIALFHLLITRTDKVRALREAFFRQNLP 243

Query: 244 NVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTLL-- 303
           NVTN  +TV +FLIVIYFQGF VVLPVRSKNARGQ+GSYPIK FYTSNMPIILQS L+  
Sbjct: 244 NVTNLHATVLIFLIVIYFQGFRVVLPVRSKNARGQRGSYPIKLFYTSNMPIILQSALVSN 303

Query: 304 ----------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANPFHA 363
                     K+GGNFLVNL+G WK SE+ SGQS+ VG +AYYITAPSSLA MA +PFHA
Sbjct: 304 IYFISQILYRKFGGNFLVNLIGTWKESEY-SGQSIPVGGIAYYITAPSSLAEMATHPFHA 363

Query: 364 MLYLVFMLSACA-FSQNY----GLKYQDLLLKI-----------ESNLQKELNRYIPTAA 423
           + YLVFML+ACA FS+ +    G   +D+  ++           +SNLQKELNRYIPTAA
Sbjct: 364 LFYLVFMLAACALFSKTWIEVSGSSAKDVARQLREQQMVMPGHRDSNLQKELNRYIPTAA 423

Query: 424 AFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFG 437
           AFGG+CIGALT            TG+LLAVTIIYQYFETFEKEKA+ELGFFG
Sbjct: 424 AFGGLCIGALTVLADLMGAIGSGTGILLAVTIIYQYFETFEKEKASELGFFG 474

BLAST of Sed0025313 vs. TAIR 10
Match: AT2G34250.1 (SecY protein transport family protein )

HSP 1 Score: 669.1 bits (1725), Expect = 2.4e-192
Identity = 359/476 (75.42%), Postives = 397/476 (83.40%), Query Frame = 0

Query: 1   MGRGFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MG GFRVLHL RPFL+FLPE +S DRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLAFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQK 120
           ADPFYW+RVILA +RG VMELGITPIVTSG+VMQLLAGSK+I+VDNNVRED ALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGLVMQLLAGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGVLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGS 180
           LLG+LIAIG A+AYVLS MYG +GQLGVGNAILII+QL F  IIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGPVGQLGVGNAILIILQLFFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQ 240
           GISLFIATNICESIIWKAFSPTTIN+GRG EFEGAVIALFH+LI +S+K+ ALR+ FYRQ
Sbjct: 181 GISLFIATNICESIIWKAFSPTTINTGRGAEFEGAVIALFHMLITKSNKVAALRQAFYRQ 240

Query: 241 NLPNVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL 300
           NLPNVTN L+TV +FLIVIYFQGF VVLPVRSKNARGQQGSYPIK FYTSNMPIILQS L
Sbjct: 241 NLPNVTNLLATVLIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 L------------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANP 360
           +            K+ GNF VNLLG WK SE+ SGQS+ V  LAY ITAP+S ++MAA+P
Sbjct: 301 VSNLYFISQLLYRKFSGNFFVNLLGQWKESEY-SGQSIPVSGLAYLITAPASFSDMAAHP 360

Query: 361 FHAMLYLVFMLSACA-FSQNY-------------GLKYQDLLL--KIESNLQKELNRYIP 420
           FHA+ Y+VFML+ACA FS+ +              LK Q +++    ESNLQKELNRYIP
Sbjct: 361 FHALFYIVFMLTACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFGF 438
           TAAAFGG+CIGALT            TG+LLAVTIIYQYFETFEKEKA+ELGFFGF
Sbjct: 421 TAAAFGGVCIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGFFGF 475

BLAST of Sed0025313 vs. TAIR 10
Match: AT2G34250.2 (SecY protein transport family protein )

HSP 1 Score: 669.1 bits (1725), Expect = 2.4e-192
Identity = 359/476 (75.42%), Postives = 397/476 (83.40%), Query Frame = 0

Query: 1   MGRGFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MG GFRVLHL RPFL+FLPE +S DRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLAFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQK 120
           ADPFYW+RVILA +RG VMELGITPIVTSG+VMQLLAGSK+I+VDNNVRED ALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGLVMQLLAGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGVLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGS 180
           LLG+LIAIG A+AYVLS MYG +GQLGVGNAILII+QL F  IIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGPVGQLGVGNAILIILQLFFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQ 240
           GISLFIATNICESIIWKAFSPTTIN+GRG EFEGAVIALFH+LI +S+K+ ALR+ FYRQ
Sbjct: 181 GISLFIATNICESIIWKAFSPTTINTGRGAEFEGAVIALFHMLITKSNKVAALRQAFYRQ 240

Query: 241 NLPNVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL 300
           NLPNVTN L+TV +FLIVIYFQGF VVLPVRSKNARGQQGSYPIK FYTSNMPIILQS L
Sbjct: 241 NLPNVTNLLATVLIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 L------------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANP 360
           +            K+ GNF VNLLG WK SE+ SGQS+ V  LAY ITAP+S ++MAA+P
Sbjct: 301 VSNLYFISQLLYRKFSGNFFVNLLGQWKESEY-SGQSIPVSGLAYLITAPASFSDMAAHP 360

Query: 361 FHAMLYLVFMLSACA-FSQNY-------------GLKYQDLLL--KIESNLQKELNRYIP 420
           FHA+ Y+VFML+ACA FS+ +              LK Q +++    ESNLQKELNRYIP
Sbjct: 361 FHALFYIVFMLTACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFGF 438
           TAAAFGG+CIGALT            TG+LLAVTIIYQYFETFEKEKA+ELGFFGF
Sbjct: 421 TAAAFGGVCIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGFFGF 475

BLAST of Sed0025313 vs. TAIR 10
Match: AT1G29310.1 (SecY protein transport family protein )

HSP 1 Score: 667.9 bits (1722), Expect = 5.5e-192
Identity = 358/476 (75.21%), Postives = 397/476 (83.40%), Query Frame = 0

Query: 1   MGRGFRVLHLARPFLSFLPEARSPDRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MG GFRVLHL RPFL+FLPE +S DRK+PFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLAFLPEVQSADRKIPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWLRVILALSRGIVMELGITPIVTSGMVMQLLAGSKLIQVDNNVREDSALLNGAQK 120
           ADPFYW+RVILA +RG VMELGITPIVTSG+VMQLLAGSK+I+VDNNVRED ALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGLVMQLLAGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGVLIAIGMAMAYVLSAMYGSIGQLGVGNAILIIVQLCFVSIIVICLDELLQKGYGLGS 180
           LLG+LIAIG A+AYVLS MYG +GQLGVGNAILII+QL F  IIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGPVGQLGVGNAILIILQLFFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICESIIWKAFSPTTINSGRGVEFEGAVIALFHLLIVRSDKIRALREEFYRQ 240
           GISLFIATNICESIIWKAFSPTTIN+GRG EFEGAVIALFH+LI +S+K+ ALR+ FYRQ
Sbjct: 181 GISLFIATNICESIIWKAFSPTTINTGRGAEFEGAVIALFHMLITKSNKVAALRQAFYRQ 240

Query: 241 NLPNVTNFLSTVFVFLIVIYFQGFWVVLPVRSKNARGQQGSYPIKQFYTSNMPIILQSTL 300
           NLPNVTN L+TV +FLIVIYFQGF VVLPVRSK+ARGQQGSYPIK FYTSNMPIILQS L
Sbjct: 241 NLPNVTNLLATVLIFLIVIYFQGFRVVLPVRSKSARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 L------------KYGGNFLVNLLGIWKVSEHSSGQSVLVGDLAYYITAPSSLANMAANP 360
           +            K+ GNF VNLLG WK SE+ SGQS+ V  LAY ITAP+S A+MAA+P
Sbjct: 301 VSNLYFISQLLYRKFSGNFFVNLLGQWKESEY-SGQSIPVSGLAYLITAPASFADMAAHP 360

Query: 361 FHAMLYLVFMLSACA-FSQNY-------------GLKYQDLLL--KIESNLQKELNRYIP 420
           FHA+ Y+VFML+ACA FS+ +              LK Q +++    ESNLQKELNRYIP
Sbjct: 361 FHALFYIVFMLTACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGMCIGALT-----------WTGVLLAVTIIYQYFETFEKEKATELGFFGF 438
           TAAAFGG+CIGALT            TG+LLAVTIIYQYFETFEKEKA+ELGFFGF
Sbjct: 421 TAAAFGGVCIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGFFGF 475

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897181.15.5e-20280.46protein transport protein Sec61 subunit alpha-like [Benincasa hispida][more]
XP_008463755.11.3e-20079.41PREDICTED: protein transport protein Sec61 subunit alpha-like [Cucumis melo] >XP... [more]
XP_030550124.18.7e-20078.99protein transport protein Sec61 subunit alpha-like [Rhodamnia argentea][more]
XP_022140644.11.5e-19979.62protein transport protein Sec61 subunit alpha-like [Momordica charantia][more]
XP_004146019.12.5e-19978.57protein transport protein Sec61 subunit alpha [Cucumis sativus] >KGN55053.1 hypo... [more]
Match NameE-valueIdentityDescription
Q54XK21.2e-14657.84Protein transport protein Sec61 subunit alpha OS=Dictyostelium discoideum OX=446... [more]
Q2KHX49.5e-14156.66Protein transport protein Sec61 subunit alpha isoform 2 OS=Bos taurus OX=9913 GN... [more]
Q9H9S39.5e-14156.66Protein transport protein Sec61 subunit alpha isoform 2 OS=Homo sapiens OX=9606 ... [more]
Q9JLR19.5e-14156.66Protein transport protein Sec61 subunit alpha isoform 2 OS=Mus musculus OX=10090... [more]
Q5NVM76.2e-14056.45Protein transport protein Sec61 subunit alpha isoform 2 OS=Pongo abelii OX=9601 ... [more]
Match NameE-valueIdentityDescription
A0A1S3CK186.5e-20179.41protein transport protein Sec61 subunit alpha-like OS=Cucumis melo OX=3656 GN=LO... [more]
A0A5A7VI036.5e-20179.41Protein transport protein Sec61 subunit alpha-like OS=Cucumis melo var. makuwa O... [more]
A0A6J1CG917.2e-20079.62protein transport protein Sec61 subunit alpha-like OS=Momordica charantia OX=367... [more]
A0A0A0L0161.2e-19978.57Plug_translocon domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G62... [more]
A0A059BMT12.1e-19978.78Plug_translocon domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGR... [more]
Match NameE-valueIdentityDescription
AT1G78720.12.1e-19676.06SecY protein transport family protein [more]
AT2G34250.12.4e-19275.42SecY protein transport family protein [more]
AT2G34250.22.4e-19275.42SecY protein transport family protein [more]
AT1G29310.15.5e-19275.21SecY protein transport family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002208SecY/SEC61-alpha familyPIRSFPIRSF004557SecY_Sec61alphacoord: 8..432
e-value: 3.3E-60
score: 201.8
IPR002208SecY/SEC61-alpha familyPFAMPF00344SecYcoord: 78..414
e-value: 1.6E-44
score: 152.4
IPR002208SecY/SEC61-alpha familyPANTHERPTHR10906SECY/SEC61-ALPHA FAMILY MEMBERcoord: 1..437
IPR019561Translocon Sec61/SecY, plug domainPFAMPF10559Plug_transloconcoord: 42..76
e-value: 9.3E-15
score: 54.2
IPR023201SecY domain superfamilyGENE3D1.10.3370.10SecY subunit domaincoord: 9..435
e-value: 7.6E-119
score: 399.2
IPR023201SecY domain superfamilySUPERFAMILY103491Preprotein translocase SecY subunitcoord: 11..427
NoneNo IPR availablePANTHERPTHR10906:SF31F9K20.24 PROTEINcoord: 1..437

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0025313.1Sed0025313.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031204 posttranslational protein targeting to membrane, translocation
biological_process GO:0006616 SRP-dependent cotranslational protein targeting to membrane, translocation
biological_process GO:0015031 protein transport
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005784 Sec61 translocon complex
cellular_component GO:0016020 membrane
molecular_function GO:0008320 protein transmembrane transporter activity
molecular_function GO:0043022 ribosome binding
molecular_function GO:0005048 signal sequence binding