Sed0024540 (gene) Chayote v1

BLAST of Sed0024540 vs. NCBI nr
Match: XP_038882517.1 (subtilisin-like protease SBT5.6 [Benincasa hispida])

HSP 1 Score: 1352.8 bits (3500), Expect = 0.0e+00
Identity = 668/784 (85.20%), Postives = 721/784 (91.96%), Query Frame = 0

Query: 1   MEISTFFCF-LLLLCSLIQ-QASSQNQKQAYIVYFGEHQGDKAWEEIEKIHHSYLLSVKD 60
           ME S+FFCF LLLL   IQ QASS +  +AYIVYFGEH GDK+WEEIE  HHSYL+SVK+
Sbjct: 1   MEKSSFFCFVLLLLLPFIQLQASSSHNHKAYIVYFGEHHGDKSWEEIEARHHSYLMSVKE 60

Query: 61  NKEDAKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTTRSWEFSGV 120
           ++EDAK SL+Y+YKH+IN FAAILTPQ+A KLSEL EVV+VIESKKY MHTTRSWEFSGV
Sbjct: 61  SEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRSWEFSGV 120

Query: 121 EEDEPRVHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGICQTGPGFES 180
           EED+P ++DLV +A +GKDVVIGMLDSGVWPKSKSFSD+GMGPIPKSWKGICQTGP F+S
Sbjct: 121 EEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAFQS 180

Query: 181 AHCNRKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRVHNVSAFGG 240
           AHCNRKIIGARYY+KGYEHHFGRLNET D+RSPCDKDGHGSHTAS AGGRRV+NVSAFGG
Sbjct: 181 AHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNVSAFGG 240

Query: 241 VARGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGVHVLSLSIG 300
           VA GTASGGAP ARLAIYKVCWAIPNQMK LGNVCFDTDMLAAMDDAIADGV VLSLSIG
Sbjct: 241 VAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLSIG 300

Query: 301 KSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGASTVDREFY 360
           KSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSN+APWIITVGASTVDREFY
Sbjct: 301 KSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGASTVDREFY 360

Query: 361 SPVILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAGSLSHEKTK 420
           SPVILGNGLKIKGLSVAPSKL K+KKMYPLVYA DI KPHVPRN+SGLCVAGSLSHEK K
Sbjct: 361 SPVILGNGLKIKGLSVAPSKL-KRKKMYPLVYAGDILKPHVPRNQSGLCVAGSLSHEKAK 420

Query: 421 GKIVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANL 480
           GKIVLC+RGEGISR+AGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDAN+
Sbjct: 421 GKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANM 480

Query: 481 ILKYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITAPGVDILAA 540
           ILKYI S   PTA IVPPITIYGSRPAPAMANFSSRGPN +DPHF+KPDITAPGVDILAA
Sbjct: 481 ILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPGVDILAA 540

Query: 541 WSEQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAAIRSALMTT 600
           WSEQDSPTKLPK LD RIVK+NLYSGTSMSCPHV+AAAALLR+IHPSWSQAAIRSALMTT
Sbjct: 541 WSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTT 600

Query: 601 STTNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDYLHYLCALN 660
           +TT NK G P+TD++SPD+ S A+PFSFGSGHFRPS A DPGLVYD+NYTDYLHYLC L 
Sbjct: 601 ATTINKSGHPLTDDASPDN-SPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLK 660

Query: 661 MNSIDPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERMVTNVGGGGKSVYFFYSRAPPG 720
           MNSIDPSF CPPRA HPHDLNYPSIAVP+LRGAVRI+R VTNVGGGGKSVYFF+S APPG
Sbjct: 661 MNSIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYFFHSEAPPG 720

Query: 721 VAVRASPNVLYFDGIGERKMFSIIVSGKVK---GKVDASGYAFGWFAWSDGIHYVRSPIA 780
           VAV ASPN+LYF+ +GERK F+I VSGKVK      + +GY+FGWFAW+DGIHYVRSPIA
Sbjct: 721 VAVSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAWTDGIHYVRSPIA 780

BLAST of Sed0024540 vs. NCBI nr
Match: XP_022926980.1 (subtilisin-like protease SBT5.6 [Cucurbita moschata])

HSP 1 Score: 1341.6 bits (3471), Expect = 0.0e+00
Identity = 661/779 (84.85%), Postives = 714/779 (91.66%), Query Frame = 0

Query: 1   MEISTFFCFLLLLCSLIQQASSQNQKQAYIVYFGEHQGDKAWEEIEKIHHSYLLSVKDNK 60
           MEIS+ FC LLLL  L+  +S   QKQAYIVYFGEH GDK+WEEIE+ HHSYL+SVKD +
Sbjct: 1   MEISSLFCLLLLLL-LLPPSSCLAQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKDTE 60

Query: 61  EDAKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTTRSWEFSGVEE 120
            DAK SL+YSYKHTINGFAA+LT Q+A KLSEL+EVV+VIESKKY MHTTRSWEFSGVEE
Sbjct: 61  NDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKKYGMHTTRSWEFSGVEE 120

Query: 121 DEPRVHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGICQTGPGFESAH 180
           D+PR+ DLVSKA  GKDVVIGMLDSGVWPKSKSFSD+GMGP+PKSWKGICQTGP FES+H
Sbjct: 121 DKPRLSDLVSKAKAGKDVVIGMLDSGVWPKSKSFSDKGMGPVPKSWKGICQTGPAFESSH 180

Query: 181 CNRKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRVHNVSAFGGVA 240
           CNRKIIGARYY+KGYEHHFGRLNET DFRSPCD DGHGSHTAS AGGRRV+NVSAFGGVA
Sbjct: 181 CNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGGVA 240

Query: 241 RGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGVHVLSLSIGKS 300
           RGTASGGAP  RLAIYKVCWAIPNQMK LGNVCFDTD+LAAMDDAI+DGV VLSLSIGKS
Sbjct: 241 RGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIGKS 300

Query: 301 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGASTVDREFYSP 360
           DPYNYTDDGIAIGALHAVKRDIVVSCSAGN GPTPS LSNVAPWIITVGASTVDREFYSP
Sbjct: 301 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFYSP 360

Query: 361 VILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAGSLSHEKTKGK 420
           VILGNGLKIKGLSVAPSKL ++ KMYPLVYAVDIQKPHVPRNESG C+AGSLSH+K KGK
Sbjct: 361 VILGNGLKIKGLSVAPSKL-RRNKMYPLVYAVDIQKPHVPRNESGSCIAGSLSHKKAKGK 420

Query: 421 IVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANLIL 480
           IVLCYRG+GISRYAGSLEVKRSGGAGMILG VPAVG R HADPHFVPATAVSY+DAN IL
Sbjct: 421 IVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRIL 480

Query: 481 KYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITAPGVDILAAWS 540
           KYI SH+ PTATIVPPITIYGSRPAPAMANF+SRGP+L+DPHF+KPDITAPGVDILAAWS
Sbjct: 481 KYIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAPGVDILAAWS 540

Query: 541 EQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAAIRSALMTTST 600
           E+DSPTKLPKS D RIVK+N+YSGTSMSCPHV+AAAALLR+IHP+WSQAAIRSALMTT+T
Sbjct: 541 EKDSPTKLPKSFDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTAT 600

Query: 601 TNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDYLHYLCALNMN 660
           T NK G PITD+S+ D+ S ATPFSFGSGHFRP+ A DPGLVYDANYTDYLHYLC L MN
Sbjct: 601 TTNKYGHPITDDSTTDN-SPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKMN 660

Query: 661 SIDPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERMVTNVGGGGKSVYFFYSRAPPGVA 720
           SID SFKCPPRA HPHDLNYPSIA+PKL+G VRI+R VTNVGGGGKSVYFF S APPGVA
Sbjct: 661 SIDRSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKSVYFFESWAPPGVA 720

Query: 721 VRASPNVLYFDGIGERKMFSIIVSGKVKGKVDASGYAFGWFAWSDGIHYVRSPIAISST 780
           VRASP+VLYFD +G+RK F+I VSGKVKG    +GY+FGWFAWSDG+HYVRSPIAISST
Sbjct: 721 VRASPSVLYFDRVGQRKRFTITVSGKVKG----NGYSFGWFAWSDGVHYVRSPIAISST 772

BLAST of Sed0024540 vs. NCBI nr
Match: KAG6594514.1 (Subtilisin-like protease 5.6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1340.5 bits (3468), Expect = 0.0e+00
Identity = 660/779 (84.72%), Postives = 714/779 (91.66%), Query Frame = 0

Query: 1   MEISTFFCFLLLLCSLIQQASSQNQKQAYIVYFGEHQGDKAWEEIEKIHHSYLLSVKDNK 60
           MEIS+ FC LLLL  L+  +S   QKQAYIVYFGEH GDK+WEEIE+ HHSYL+SVKD +
Sbjct: 1   MEISSLFC-LLLLVLLLPPSSCLAQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKDTE 60

Query: 61  EDAKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTTRSWEFSGVEE 120
            DAK SL+YSYKHTINGFAA+LT Q+A KLSEL+EVV+VIESKKY MHTTRSWEFSGV+E
Sbjct: 61  NDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKKYGMHTTRSWEFSGVQE 120

Query: 121 DEPRVHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGICQTGPGFESAH 180
           D+PR+ DLVSKA  GKDVVIGMLDSGVWPKSKSFSD+GMGP+PKSWKGICQTGP FES+H
Sbjct: 121 DKPRLSDLVSKAKSGKDVVIGMLDSGVWPKSKSFSDKGMGPVPKSWKGICQTGPAFESSH 180

Query: 181 CNRKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRVHNVSAFGGVA 240
           CNRKIIGARYY+KGYEHHFGRLNET DFRSPCD DGHGSHTAS AGGRRV+NVSAFGGVA
Sbjct: 181 CNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGGVA 240

Query: 241 RGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGVHVLSLSIGKS 300
           RGTASGGAP  RLAIYKVCWAIPNQMK LGNVCFDTD+LAAMDDAI+DGV VLSLSIGKS
Sbjct: 241 RGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIGKS 300

Query: 301 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGASTVDREFYSP 360
           DPYNYTDDGIAIGALHAVKRDIVVSCSAGN GPTPS LSNVAPWIITVGASTVDREFYSP
Sbjct: 301 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFYSP 360

Query: 361 VILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAGSLSHEKTKGK 420
           VILGNGLKIKGLSVAPSKL ++ KMYPLVYAVDIQKPHVPRNESG C+AGSLSH+K KGK
Sbjct: 361 VILGNGLKIKGLSVAPSKL-RRNKMYPLVYAVDIQKPHVPRNESGSCIAGSLSHKKAKGK 420

Query: 421 IVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANLIL 480
           IVLCYRG+GISRYAGSLEVKRSGGAGMILG VPAVG R HADPHFVPATAVSY+DAN IL
Sbjct: 421 IVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRIL 480

Query: 481 KYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITAPGVDILAAWS 540
           KYI SH+ PTATIVPPITIYGSRPAPAMANF+SRGP+L+DPHF+KPDITAPGVDILAAWS
Sbjct: 481 KYIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAPGVDILAAWS 540

Query: 541 EQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAAIRSALMTTST 600
           E+DSPTKLPKS D RIVK+N+YSGTSMSCPHV+AAAALLR+IHP+WSQAAIRSALMTT+T
Sbjct: 541 EKDSPTKLPKSFDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTAT 600

Query: 601 TNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDYLHYLCALNMN 660
           T NK G PITD+S+ D+ S ATPFSFGSGHFRP+ A DPGLVYDANYTDYLHYLC L MN
Sbjct: 601 TTNKYGHPITDDSTTDN-SPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKMN 660

Query: 661 SIDPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERMVTNVGGGGKSVYFFYSRAPPGVA 720
           SID SFKCPPRA HPHDLNYPSIA+PKL+G VRI+R VTNVGGGGKSVYFF S APPGVA
Sbjct: 661 SIDRSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKSVYFFESWAPPGVA 720

Query: 721 VRASPNVLYFDGIGERKMFSIIVSGKVKGKVDASGYAFGWFAWSDGIHYVRSPIAISST 780
           VRASP+VLYFD +G+RK F+I VSGKVKG    +GY+FGWFAWSDG+HYVRSPIAISST
Sbjct: 721 VRASPSVLYFDRVGQRKRFTITVSGKVKG----NGYSFGWFAWSDGVHYVRSPIAISST 772

BLAST of Sed0024540 vs. NCBI nr
Match: XP_023517293.1 (subtilisin-like protease SBT5.6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 659/780 (84.49%), Postives = 713/780 (91.41%), Query Frame = 0

Query: 1   MEISTFFCFLLLLCSLIQQASS-QNQKQAYIVYFGEHQGDKAWEEIEKIHHSYLLSVKDN 60
           ME S+ FC LLLL  L+   SS   QKQAYIVYFGEH GDK+WEEIE+ HHSYL+SVKD 
Sbjct: 1   METSSLFCLLLLLLLLLLPPSSCLAQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKDT 60

Query: 61  KEDAKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTTRSWEFSGVE 120
           + DAK SL+YSYKHTINGFAA+LT Q+A KLSEL+EVV+VIESK+Y MHTTRSWEFSGVE
Sbjct: 61  ENDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKRYGMHTTRSWEFSGVE 120

Query: 121 EDEPRVHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGICQTGPGFESA 180
           ED+PR+ DLVSKA  GKDVVIGMLDSGVWPKSKSFSD+GMGP+PKSWKGICQTGPGF+S 
Sbjct: 121 EDKPRLSDLVSKAKSGKDVVIGMLDSGVWPKSKSFSDKGMGPVPKSWKGICQTGPGFQSP 180

Query: 181 HCNRKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRVHNVSAFGGV 240
           HCNRKIIGARYY+KGYEHHFGRLNET DFRSPCD DGHGSHTAS AGGRRV+NVSAFGGV
Sbjct: 181 HCNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGGV 240

Query: 241 ARGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGVHVLSLSIGK 300
           ARGTASGGAP  RLAIYKVCWAIPNQMK LGNVCFDTD+LAAMDDAI+DGV VLSLSIGK
Sbjct: 241 ARGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIGK 300

Query: 301 SDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGASTVDREFYS 360
           SDPYNYTDDGIAIGALHAVKRDIVVSCSAGN GPTPS LSNVAPWIITVGASTVDREFYS
Sbjct: 301 SDPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFYS 360

Query: 361 PVILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAGSLSHEKTKG 420
           PVILGNGLKIKGLSVAPSKL ++ KMYPLVYAVDIQKPHVPRNESG C+AGSLSH+K KG
Sbjct: 361 PVILGNGLKIKGLSVAPSKL-RRNKMYPLVYAVDIQKPHVPRNESGSCIAGSLSHKKAKG 420

Query: 421 KIVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANLI 480
           KIVLCYRG+GISRYAGSLEVKRSGGAGMILG VPAVG R HADPHFVPATAVSY+DAN I
Sbjct: 421 KIVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRI 480

Query: 481 LKYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITAPGVDILAAW 540
           LKYI SH+ PTATIVPPITIYGSRPAPAMA F+SRGP+L+DPHF+KPDITAPGVDILAAW
Sbjct: 481 LKYIKSHRNPTATIVPPITIYGSRPAPAMAYFTSRGPSLVDPHFLKPDITAPGVDILAAW 540

Query: 541 SEQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAAIRSALMTTS 600
           SE+DSPTKLPKS D RIVK+N+YSGTSMSCPHV+AAAALLR+IHP+WSQAAIRSALMTT+
Sbjct: 541 SEKDSPTKLPKSFDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTA 600

Query: 601 TTNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDYLHYLCALNM 660
           TT NK G PITD+S+ D+ S ATPFSFGSGHFRP+ A DPGLVYDANYTDYLHYLC L M
Sbjct: 601 TTTNKYGHPITDDSTTDN-SPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKM 660

Query: 661 NSIDPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERMVTNVGGGGKSVYFFYSRAPPGV 720
           NSIDPSFKCPPRA HPHDLNYPSIA+PKL+G VRI+R VTNVGGGGK+VYFF S APPGV
Sbjct: 661 NSIDPSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKTVYFFESWAPPGV 720

Query: 721 AVRASPNVLYFDGIGERKMFSIIVSGKVKGKVDASGYAFGWFAWSDGIHYVRSPIAISST 780
           AVRASP+VLYFD +G+RK F++ VSGKVKG    +GY+FGWFAWSDGIHYVRSPIAISST
Sbjct: 721 AVRASPSVLYFDRVGQRKRFTVTVSGKVKG----NGYSFGWFAWSDGIHYVRSPIAISST 774

BLAST of Sed0024540 vs. NCBI nr
Match: XP_023003763.1 (subtilisin-like protease SBT5.6 [Cucurbita maxima] >XP_023003764.1 subtilisin-like protease SBT5.6 [Cucurbita maxima])

HSP 1 Score: 1335.9 bits (3456), Expect = 0.0e+00
Identity = 660/779 (84.72%), Postives = 709/779 (91.01%), Query Frame = 0

Query: 1   MEISTFFCFLLLLCSLIQQASSQNQKQAYIVYFGEHQGDKAWEEIEKIHHSYLLSVKDNK 60
           ME ST FC LLLL  L+  +S   QKQAYIVYFGEH GDK+WEEIE+ HHSYL+SVKD +
Sbjct: 1   METSTLFCLLLLL--LLPPSSCLAQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKDTE 60

Query: 61  EDAKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTTRSWEFSGVEE 120
            DAK SL+YSYKHTINGFAA+LT Q+A KLSEL+EVV+VIESK Y MHTTRSWEFSGV E
Sbjct: 61  NDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKTYGMHTTRSWEFSGVVE 120

Query: 121 DEPRVHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGICQTGPGFESAH 180
           D+PR+ DLVSKA  GKDVVIGMLDSGVWPKSKSFSD GMGP+PKSWKGICQTGPGFES H
Sbjct: 121 DKPRLSDLVSKAKSGKDVVIGMLDSGVWPKSKSFSDNGMGPVPKSWKGICQTGPGFESPH 180

Query: 181 CNRKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRVHNVSAFGGVA 240
           CNRKIIGARYY+KGYEHHFGRLNET DFRSPCD DGHGSHTAS AGGRRV+NVSAFGGVA
Sbjct: 181 CNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGGVA 240

Query: 241 RGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGVHVLSLSIGKS 300
           RGTASGGAP  RLAIYKVCWAIPNQMK LGNVCFDTD+LAAMDDAI+DGV VLSLSIGKS
Sbjct: 241 RGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIGKS 300

Query: 301 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGASTVDREFYSP 360
           DPYNYTDDGIAIGALHAVKRDIVVSCSAGN GPTPS LSNVAPWIITVGASTVDREFYSP
Sbjct: 301 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFYSP 360

Query: 361 VILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAGSLSHEKTKGK 420
           VILGNGLKIKGLSVAPSKL ++ KMYPLVYAVDIQKPHVP+NESG C+AGSLSH+K KGK
Sbjct: 361 VILGNGLKIKGLSVAPSKL-RRNKMYPLVYAVDIQKPHVPQNESGSCIAGSLSHKKAKGK 420

Query: 421 IVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANLIL 480
           IVLCYRG+GISRYAGSLEVKRSGGAGMILG VPAVG R HADPHFVPATAVSY+DAN IL
Sbjct: 421 IVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRIL 480

Query: 481 KYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITAPGVDILAAWS 540
           KYI SH+ PTATIVPPITIYGSRPAPAMANF+SRGP+L+DPHF+KPDITAPGVDILAAWS
Sbjct: 481 KYIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAPGVDILAAWS 540

Query: 541 EQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAAIRSALMTTST 600
           E+DSPT LPK LD RIVK+N+YSGTSMSCPHV+AAAALLR+IHP+WSQAAIRSALMTTST
Sbjct: 541 EKDSPTXLPKFLDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTST 600

Query: 601 TNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDYLHYLCALNMN 660
           T NK G PITD+S+ D+ S ATPFSFGSGHFRP+ A DPGLVYDANYTDYLHYLC L MN
Sbjct: 601 TTNKYGHPITDDSTSDN-SPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKMN 660

Query: 661 SIDPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERMVTNVGGGGKSVYFFYSRAPPGVA 720
           SID SFKCPPRA HPHDLNYPSIA+PKL+G VRI+R VTNVGGGGKSVYFF S APPGVA
Sbjct: 661 SIDRSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKSVYFFESWAPPGVA 720

Query: 721 VRASPNVLYFDGIGERKMFSIIVSGKVKGKVDASGYAFGWFAWSDGIHYVRSPIAISST 780
           VRASP+VLYFD +G+RK F++ VSGKVKG    SGY+FGWFAWSDGIHYVRSPIAISST
Sbjct: 721 VRASPSVLYFDRVGQRKRFTVTVSGKVKG----SGYSFGWFAWSDGIHYVRSPIAISST 771

BLAST of Sed0024540 vs. ExPASy Swiss-Prot
Match: Q9FK76 (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 4.8e-252
Identity = 448/792 (56.57%), Postives = 567/792 (71.59%), Query Frame = 0

Query: 10  LLLLCSLIQQASSQNQKQAYIVYFGEHQGDKAWEEIEKIHHSYLLSVKDNKEDAKFSLIY 69
           LL L  L+  AS   +KQ YIVYFGEH+GDKA+ EIE+ HHSYL SVK+++EDA+ SL+Y
Sbjct: 10  LLFLIPLL--ASCAEEKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSVKESEEDARASLLY 69

Query: 70  SYKHTINGFAAILTPQEAFKLSELKEVVTVIES--KKYSMHTTRSWEFSGVEEDE----- 129
           SYKH+INGFAA LTP +A KL +L EVV+V +S  +KY  HTTRSWEF G+EE+E     
Sbjct: 70  SYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDV 129

Query: 130 PR-----------VHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGICQ 189
           PR             + + KA HG  +++G+LDSGVWP+SKSF+D+GMGP+PKSWKGICQ
Sbjct: 130 PRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGICQ 189

Query: 190 TGPGFESAHCNRKIIGARYYIKGYEHHFGRLNETI--DFRSPCDKDGHGSHTASTAGGRR 249
           TG  F S+HCNRKIIGARYY+KGYE ++G  N T   DF SP D DGHGSHTASTA GRR
Sbjct: 190 TGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGRR 249

Query: 250 VHNVSAFGGVARGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADG 309
           V   SA GG A+G+ASGGAP+ARLAIYK CWA PN  K+ GN+C + DMLAA+DDAIADG
Sbjct: 250 VLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADG 309

Query: 310 VHVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVG 369
           VHV+S+SIG ++P+ +T DGIA+GALHAVKR+IVV+ SAGN GP P  LSN+APWIITVG
Sbjct: 310 VHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVG 369

Query: 370 ASTVDREFYSPVILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVA 429
           AST+DR F   ++LGNG  IK  S+   K+    K  PLVYA ++  P +  NE+  C+ 
Sbjct: 370 ASTLDRAFVGGLVLGNGYTIKTDSITAFKM---DKFAPLVYASNVVVPGIALNETSQCLP 429

Query: 430 GSLSHEKTKGKIVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPAT 489
            SL  E   GK+VLC RG G SR    +EVKR+GGAGMILGN+ A G    +D HFVP  
Sbjct: 430 NSLKPELVSGKVVLCLRGAG-SRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPTA 489

Query: 490 AVSYEDANLILKYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDIT 549
            V+    + IL+YI + K P A I P  T+Y  + AP+M  FSSRGPN++DP+ +KPDIT
Sbjct: 490 GVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDIT 549

Query: 550 APGVDILAAWSEQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQA 609
           APG+ ILAAWS  DSP+K+  S+D R+  +N+YSGTSMSCPHVA A ALL++IHP WS A
Sbjct: 550 APGLYILAAWSGADSPSKM--SVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSSA 609

Query: 610 AIRSALMTTSTTNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTD 669
           AIRSALMTT+   N + +PI D +       A PF+ GSGHFRP+ A DPGLVYDA+Y  
Sbjct: 610 AIRSALMTTAWMTNDKKKPIQDTTG----LPANPFALGSGHFRPTKAADPGLVYDASYRA 669

Query: 670 YLHYLCALNMNSIDPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERMVTNVG-GGGKSV 729
           YL Y C++N+ +IDP+FKCP +    ++ NYPSIAVP L+  V ++R VTNVG G   S 
Sbjct: 670 YLLYGCSVNITNIDPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGNSTST 729

Query: 730 YFFYSRAPPGVAVRASPNVLYFDGIGERKMFSIIV---SGKVKGKVDASGYAFGWFAWSD 778
           Y F  + P G++V+A PN+L F+ IG+++ F I++     +V    +   Y FGWF+W+D
Sbjct: 730 YLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWFSWTD 789

BLAST of Sed0024540 vs. ExPASy Swiss-Prot
Match: F4KEL0 (Subtilisin-like protease SBT5.5 OS=Arabidopsis thaliana OX=3702 GN=SBT5.5 PE=3 SV=2)

HSP 1 Score: 795.4 bits (2053), Expect = 5.7e-229
Identity = 433/804 (53.86%), Postives = 544/804 (67.66%), Query Frame = 0

Query: 7   FCFLLLLCSLIQ-QASSQNQKQAYIVYFGEHQGDKAWEEIEKIHHSYLLSVKDNKEDAKF 66
           F FL LL  L+   AS   +KQ YIVYFGEH+GDKA+ EIE  HHSYL SVK+ +EDA  
Sbjct: 8   FIFLSLLLFLVPLLASCTKEKQVYIVYFGEHKGDKAFHEIEAHHHSYLQSVKETEEDATS 67

Query: 67  SLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIES--KKYSMHTTRSWEFSGVEEDE- 126
           SL+Y    +INGFAA LTP +A +L ELKEVV+V +S  +KY +HTTRSWEF G++E+E 
Sbjct: 68  SLLYRRASSINGFAAELTPDQASRLKELKEVVSVFKSDPRKYKIHTTRSWEFVGLKEEEG 127

Query: 127 ---------PR----VHD-------LVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPI 186
                    PR    V+D        +  A HG  V++G++DSGVWP+S+SF D+GMGPI
Sbjct: 128 EDYRSDGDAPRHKYDVNDRFRVGRKFLKNAKHGDGVIVGLIDSGVWPESRSFDDKGMGPI 187

Query: 187 PKSWKGICQTGPGFESAHCNRKIIGARYYIKGYEHHFGRLNETI--DFRSPCDKDGHGSH 246
           P+SWKGICQTG  F S+HCNRKIIGARYY +GYE ++G  N     DF SP D DGHGSH
Sbjct: 188 PESWKGICQTGVAFNSSHCNRKIIGARYYARGYERYYGPFNAEANKDFLSPRDADGHGSH 247

Query: 247 TASTAGGRRVHNVSAFGGVARGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLA 306
           TASTA GRRV  VSA GG+A GTASGGA +ARLA+YK CWA+PN+ K   N CFD DMLA
Sbjct: 248 TASTAVGRRVDGVSALGGIAMGTASGGASLARLAVYKACWAVPNKEKYATNTCFDEDMLA 307

Query: 307 AMDDAIADGVHVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSN 366
           A DDAIADGV+V+S+SIG  +P+ Y +DGIAIGALHAVKRDIVV+ SAGN GP    LSN
Sbjct: 308 AFDDAIADGVNVISISIGTVEPHTYLEDGIAIGALHAVKRDIVVAASAGNDGPARETLSN 367

Query: 367 VAPWIITVGASTVDREFYSPVILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVP 426
            APWIITVGAS++DR F   + LG+G   +  S+   K+       PLVYA D+  P V 
Sbjct: 368 PAPWIITVGASSLDRFFVGRLELGDGYVFESDSLTTLKM---DNYAPLVYAPDVVVPGVS 427

Query: 427 RNESGLCVAGSLSHEKTKGKIVLCYRGEGISRYAG-SLEVKRSGGAGMILGNVPAVGRRP 486
           RN++ LC+  +LS +  +GK+VLC RG G     G  LEVKR+GG GMIL N        
Sbjct: 428 RNDAMLCLPNALSPDHVRGKVVLCLRGYGSGSTIGKGLEVKRAGGVGMILAN-SRDNDAF 487

Query: 487 HADPHFVPATAVSYEDANLILKYIMSHKKPTATIVPPITI-YGSRPAPAMANFSSRGPNL 546
             + HFVP   V     + IL YI +  +P A I P  T+ Y ++P  ++  FSSR PN 
Sbjct: 488 DVESHFVPTALVFSSTVDRILDYIYNTYEPVAFIKPAETVLYRNQPEDSVYPFSSRAPNW 547

Query: 547 IDPHFIKPDITAPGVDILAAWSEQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAAL 606
           +D + +KPDI APG++ILAAWS  DS +K   S+D R++ +NL SGTSMSCPHVA A AL
Sbjct: 548 VDANILKPDIIAPGLNILAAWSGADSASK--DSIDRRVLDYNLDSGTSMSCPHVAGAIAL 607

Query: 607 LRSIHPSWSQAAIRSALMTTSTTNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVD 666
           L+S+HP+WS AAIRSALMTT++  N+  +PI D       S A PF+ GS HFRP+ A  
Sbjct: 608 LKSMHPTWSSAAIRSALMTTASMTNEDNEPIQDYDG----SPANPFALGSRHFRPTKAAS 667

Query: 667 PGLVYDANYTDYLHYLCALNMNSIDPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERMV 726
           PGLVYDA+Y  YL Y C++ + ++DP+FKCP R    ++LNYPSI++P L G V + R V
Sbjct: 668 PGLVYDASYQSYLLYCCSVGLTNLDPTFKCPSRIPPGYNLNYPSISIPYLSGTVTVTRTV 727

Query: 727 TNVG--GGGKSVYFFYSRAPPGVAVRASPNVLYFDGIGERKMFSIIVSG---KVKGKVDA 778
           T VG  G   SVY F ++ P GV V+A PNVL FD IG++K F+II +    +  G+   
Sbjct: 728 TCVGRTGNSTSVYVFNAQPPNGVLVKAEPNVLVFDKIGQKKRFNIIFTTQRYEFTGEARR 787

BLAST of Sed0024540 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 2.5e-176
Identity = 364/793 (45.90%), Postives = 485/793 (61.16%), Query Frame = 0

Query: 1   MEISTFFCFLLLLCSLIQQAS----SQNQKQAYIVYFGEHQ-----GDKAWEEIEKIHHS 60
           M+++  F FLLLL  L+  +S    +     +Y+VYFG H       + A + +++ H+ 
Sbjct: 1   MKLTHNFSFLLLLL-LVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYD 60

Query: 61  YLLSVKDNKEDAKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTTR 120
           +L S   ++E A  ++ YSY   INGFAA L    A+++S+  EVV+V  +K   +HTTR
Sbjct: 61  FLGSFTGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTR 120

Query: 121 SWEFSGVEEDE-PRVHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGIC 180
           SW+F G+E +       +  KA  G+D +I  LD+GVWP+SKSF DEG+GPIP  WKGIC
Sbjct: 121 SWDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGIC 180

Query: 181 QTGPGFESAHCNRKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRV 240
           Q      + HCNRK+IGARY+ KGY    G LN + D  SP D DGHGSHT STA G  V
Sbjct: 181 QNQKD-ATFHCNRKLIGARYFNKGYAAAVGHLNSSFD--SPRDLDGHGSHTLSTAAGDFV 240

Query: 241 HNVSAFGGVARGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGV 300
             VS F G   GTA GG+P AR+A YKVCW       + GN C+D D+LAA D AI DG 
Sbjct: 241 PGVSIF-GQGNGTAKGGSPRARVAAYKVCWP-----PVKGNECYDADVLAAFDAAIHDGA 300

Query: 301 HVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360
            V+S+S+G  +P ++ +D +AIG+ HA K+ IVV CSAGN GP  S +SNVAPW ITVGA
Sbjct: 301 DVISVSLG-GEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGA 360

Query: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAG 420
           ST+DREF S ++LGNG   KG S++ + L    K YP++ +V+ +  +    ++ LC  G
Sbjct: 361 STMDREFASNLVLGNGKHYKGQSLSSTAL-PHAKFYPIMASVNAKAKNASALDAQLCKLG 420

Query: 421 SLSHEKTKGKIVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATA 480
           SL   KTKGKI++C RG+   R      V   GG GM+L N    G    ADPH +PAT 
Sbjct: 421 SLDPIKTKGKILVCLRGQN-GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQ 480

Query: 481 VSYEDANLILKYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITA 540
           ++ +D+  + +YI   KKP A I P  T  G +PAP MA+FSS+GP+++ P  +KPDITA
Sbjct: 481 LTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITA 540

Query: 541 PGVDILAAWSEQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAA 600
           PGV ++AA++   SPT   +  D R + FN  SGTSMSCPH++  A LL++ +PSWS AA
Sbjct: 541 PGVSVIAAYTGAVSPTN--EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAA 600

Query: 601 IRSALMTTSTTNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDY 660
           IRSA+MTT+T  +    PI + ++      ATPFSFG+GH +P+ AV+PGLVYD    DY
Sbjct: 601 IRSAIMTTATIMDDIPGPIQNATN----MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDY 660

Query: 661 LHYLCALNMNSIDPS------FKCPPRARHPHDLNYPSIAVPKLRGA-VRIERMVTNVGG 720
           L++LC+L  N+   S      F C        +LNYPSI VP L  + V + R V NV  
Sbjct: 661 LNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNV-- 720

Query: 721 GGKSVYFFYSRAPPGVAVRASPNVLYFDGIGERKMFSIIVSGKVKGKVDASGYAFGWFAW 777
           G  S+Y      P GV V   P  L F  +GE+K F +I+  K KG V A GY FG   W
Sbjct: 721 GRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILV-KSKGNV-AKGYVFGELVW 770

BLAST of Sed0024540 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 6.0e-170
Identity = 351/790 (44.43%), Postives = 466/790 (58.99%), Query Frame = 0

Query: 1   MEISTFFCFLLLLCSLIQQASSQNQKQAYIVYFGEHQ-----GDKAWEEIEKIHHSYLLS 60
           M + +    LLL+      A +   K++YIVY G H           + +   H ++L S
Sbjct: 16  MSLQSLSSLLLLVTLFFSPAFA--LKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLAS 75

Query: 61  VKDNKEDAKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTTRSWEF 120
              + E+AK ++ YSYK  INGFAAIL   EA ++++  +VV+V  +K   +HTT SW F
Sbjct: 76  FVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNF 135

Query: 121 SGVEEDEPRVH--DLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGICQTG 180
             + ++   VH   L +KA +G+D +I  LD+GVWP+SKSFSDEG G +P  WKG C   
Sbjct: 136 MLLAKNGV-VHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH-- 195

Query: 181 PGFESAHCNRKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRVHNV 240
              +   CNRK+IGARY+ KGY  + G L     + +  D DGHGSHT STA G  V   
Sbjct: 196 ---KDVPCNRKLIGARYFNKGYLAYTG-LPSNASYETCRDHDGHGSHTLSTAAGNFVPGA 255

Query: 241 SAFGGVARGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGVHVL 300
           + F G+  GTASGG+P AR+A YKVCW       + G  CFD D+LAA++ AI DGV VL
Sbjct: 256 NVF-GIGNGTASGGSPKARVAAYKVCWP-----PVDGAECFDADILAAIEAAIEDGVDVL 315

Query: 301 SLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGASTV 360
           S S+G  D  +Y  DGIAIG+ HAVK  + V CSAGN GP    +SNVAPW+ITVGAS++
Sbjct: 316 SASVG-GDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSM 375

Query: 361 DREFYSPVILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAGSLS 420
           DREF + V L NG   KG S+  SK   ++KMY L+ A D    +    ++ LC  GSL 
Sbjct: 376 DREFQAFVELKNGQSFKGTSL--SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLD 435

Query: 421 HEKTKGKIVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSY 480
            +K KGKI++C RG+  +R    ++   +G AGM+L N  A G    +D H +PA+ + Y
Sbjct: 436 PKKVKGKILVCLRGDN-ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDY 495

Query: 481 EDANLILKYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITAPGV 540
           +D   +  Y+ S K P   I  P     ++PAP MA+FSSRGPN I P  +KPDITAPGV
Sbjct: 496 KDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGV 555

Query: 541 DILAAWSEQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAAIRS 600
           +I+AA++E   PT L    D+R   FN  SGTSMSCPH++    LL+++HP WS AAIRS
Sbjct: 556 NIIAAFTEATGPTDLDS--DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRS 615

Query: 601 ALMTTSTTNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDYLHY 660
           A+MTTS T N R +P+ D    +S   A PFS+GSGH +P+ A  PGLVYD    DYL +
Sbjct: 616 AIMTTSRTRNNRRKPMVD----ESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDF 675

Query: 661 LCALNMNSI-------DPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERMVTNVGGGGK 720
           LCA+  N+        DP + C   A +  D NYPSI VP L G++ + R + NV  G  
Sbjct: 676 LCAVGYNNTVVQLFAEDPQYTCRQGA-NLLDFNYPSITVPNLTGSITVTRKLKNV--GPP 735

Query: 721 SVYFFYSRAPPGVAVRASPNVLYFDGIGERKMFSIIVSGKVKGKVDASGYAFGWFAWSDG 777
           + Y    R P GV V   P  L F+  GE K+F + +       V  SGY FG   W+D 
Sbjct: 736 ATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPL---PVTPSGYVFGELTWTDS 774

BLAST of Sed0024540 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 4.7e-167
Identity = 351/785 (44.71%), Postives = 475/785 (60.51%), Query Frame = 0

Query: 3   ISTFFCFLLLLCSLIQQASSQNQKQAYIVYFGEHQGDKAWEEIEKIHHSYLLSVKDNKED 62
           +S+   FLLL       +SS + +  YIV+  + Q   +++     + S L S+ D+ E 
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE- 65

Query: 63  AKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTTRSWEFSGVEEDE 122
               L+Y+Y++ I+GF+  LT +EA  L     V++V+   +Y +HTTR+  F G++E  
Sbjct: 66  ----LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE-- 125

Query: 123 PRVHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGICQTGPGFESAHCN 182
               DL  +A    DVV+G+LD+GVWP+SKS+SDEG GPIP SWKG C+ G  F ++ CN
Sbjct: 126 -HTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCN 185

Query: 183 RKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRVHNVSAFGGVARG 242
           RK+IGAR++ +GYE   G ++E+ + RSP D DGHG+HT+STA G  V   S   G A G
Sbjct: 186 RKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL-GYASG 245

Query: 243 TASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGVHVLSLSIGKSDP 302
           TA G AP AR+A+YKVCW        LG  CF +D+LAA+D AIAD V+VLS+S+G    
Sbjct: 246 TARGMAPRARVAVYKVCW--------LGG-CFSSDILAAIDKAIADNVNVLSMSLG-GGM 305

Query: 303 YNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGASTVDREFYSPVI 362
            +Y  DG+AIGA  A++R I+VSCSAGN GP+ S+LSNVAPWI TVGA T+DR+F +  I
Sbjct: 306 SDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAI 365

Query: 363 LGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAGSLSHEKTKGKIV 422
           LGNG    G+S+   +     K+ P +YA +            LC+ G+L  EK KGKIV
Sbjct: 366 LGNGKNFTGVSLFKGE-ALPDKLLPFIYAGNASN----ATNGNLCMTGTLIPEKVKGKIV 425

Query: 423 LCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANLILKY 482
           +C RG       G + VK +GG GMIL N  A G    AD H +PAT V  +  ++I  Y
Sbjct: 426 MCDRGINARVQKGDV-VKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHY 485

Query: 483 IMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITAPGVDILAAWSEQ 542
           + +   PTA+I    T+ G +P+P +A FSSRGPN I P+ +KPD+ APGV+ILAAW+  
Sbjct: 486 VTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGA 545

Query: 543 DSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAAIRSALMTTSTTN 602
             PT L  + DSR V+FN+ SGTSMSCPHV+  AALL+S+HP WS AAIRSALMTT+   
Sbjct: 546 AGPTGL--ASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKT 605

Query: 603 NKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDYLHYLCALNMNSI 662
            K G+P+ D ++      +TPF  G+GH  P+ A +PGL+YD    DYL +LCALN  S 
Sbjct: 606 YKDGKPLLDIATG---KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSP 665

Query: 663 D------PSFKC-PPRARHPHDLNYPSIAV-PKLRGAVRIERMVTNVGGGGKSVYFFYSR 722
                   ++ C P ++    DLNYPS AV     GA +  R VT+VGG G       S 
Sbjct: 666 QIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSE 725

Query: 723 APPGVAVRASPNVLYFDGIGERKMFSIIVSGKVKGKVDASGYAFGWFAWSDGIHYVRSPI 780
              GV +   P VL F    E+K +++  +  V     +   +FG   WSDG H V SP+
Sbjct: 726 T-TGVKISVEPAVLNFKEANEKKSYTVTFT--VDSSKPSGSNSFGSIEWSDGKHVVGSPV 757

BLAST of Sed0024540 vs. ExPASy TrEMBL
Match: A0A6J1EMK8 (subtilisin-like protease SBT5.6 OS=Cucurbita moschata OX=3662 GN=LOC111433935 PE=3 SV=1)

HSP 1 Score: 1341.6 bits (3471), Expect = 0.0e+00
Identity = 661/779 (84.85%), Postives = 714/779 (91.66%), Query Frame = 0

Query: 1   MEISTFFCFLLLLCSLIQQASSQNQKQAYIVYFGEHQGDKAWEEIEKIHHSYLLSVKDNK 60
           MEIS+ FC LLLL  L+  +S   QKQAYIVYFGEH GDK+WEEIE+ HHSYL+SVKD +
Sbjct: 1   MEISSLFCLLLLLL-LLPPSSCLAQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKDTE 60

Query: 61  EDAKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTTRSWEFSGVEE 120
            DAK SL+YSYKHTINGFAA+LT Q+A KLSEL+EVV+VIESKKY MHTTRSWEFSGVEE
Sbjct: 61  NDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKKYGMHTTRSWEFSGVEE 120

Query: 121 DEPRVHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGICQTGPGFESAH 180
           D+PR+ DLVSKA  GKDVVIGMLDSGVWPKSKSFSD+GMGP+PKSWKGICQTGP FES+H
Sbjct: 121 DKPRLSDLVSKAKAGKDVVIGMLDSGVWPKSKSFSDKGMGPVPKSWKGICQTGPAFESSH 180

Query: 181 CNRKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRVHNVSAFGGVA 240
           CNRKIIGARYY+KGYEHHFGRLNET DFRSPCD DGHGSHTAS AGGRRV+NVSAFGGVA
Sbjct: 181 CNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGGVA 240

Query: 241 RGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGVHVLSLSIGKS 300
           RGTASGGAP  RLAIYKVCWAIPNQMK LGNVCFDTD+LAAMDDAI+DGV VLSLSIGKS
Sbjct: 241 RGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIGKS 300

Query: 301 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGASTVDREFYSP 360
           DPYNYTDDGIAIGALHAVKRDIVVSCSAGN GPTPS LSNVAPWIITVGASTVDREFYSP
Sbjct: 301 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFYSP 360

Query: 361 VILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAGSLSHEKTKGK 420
           VILGNGLKIKGLSVAPSKL ++ KMYPLVYAVDIQKPHVPRNESG C+AGSLSH+K KGK
Sbjct: 361 VILGNGLKIKGLSVAPSKL-RRNKMYPLVYAVDIQKPHVPRNESGSCIAGSLSHKKAKGK 420

Query: 421 IVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANLIL 480
           IVLCYRG+GISRYAGSLEVKRSGGAGMILG VPAVG R HADPHFVPATAVSY+DAN IL
Sbjct: 421 IVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRIL 480

Query: 481 KYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITAPGVDILAAWS 540
           KYI SH+ PTATIVPPITIYGSRPAPAMANF+SRGP+L+DPHF+KPDITAPGVDILAAWS
Sbjct: 481 KYIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAPGVDILAAWS 540

Query: 541 EQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAAIRSALMTTST 600
           E+DSPTKLPKS D RIVK+N+YSGTSMSCPHV+AAAALLR+IHP+WSQAAIRSALMTT+T
Sbjct: 541 EKDSPTKLPKSFDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTAT 600

Query: 601 TNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDYLHYLCALNMN 660
           T NK G PITD+S+ D+ S ATPFSFGSGHFRP+ A DPGLVYDANYTDYLHYLC L MN
Sbjct: 601 TTNKYGHPITDDSTTDN-SPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKMN 660

Query: 661 SIDPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERMVTNVGGGGKSVYFFYSRAPPGVA 720
           SID SFKCPPRA HPHDLNYPSIA+PKL+G VRI+R VTNVGGGGKSVYFF S APPGVA
Sbjct: 661 SIDRSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKSVYFFESWAPPGVA 720

Query: 721 VRASPNVLYFDGIGERKMFSIIVSGKVKGKVDASGYAFGWFAWSDGIHYVRSPIAISST 780
           VRASP+VLYFD +G+RK F+I VSGKVKG    +GY+FGWFAWSDG+HYVRSPIAISST
Sbjct: 721 VRASPSVLYFDRVGQRKRFTITVSGKVKG----NGYSFGWFAWSDGVHYVRSPIAISST 772

BLAST of Sed0024540 vs. ExPASy TrEMBL
Match: A0A6J1KU93 (subtilisin-like protease SBT5.6 OS=Cucurbita maxima OX=3661 GN=LOC111497250 PE=3 SV=1)

HSP 1 Score: 1335.9 bits (3456), Expect = 0.0e+00
Identity = 660/779 (84.72%), Postives = 709/779 (91.01%), Query Frame = 0

Query: 1   MEISTFFCFLLLLCSLIQQASSQNQKQAYIVYFGEHQGDKAWEEIEKIHHSYLLSVKDNK 60
           ME ST FC LLLL  L+  +S   QKQAYIVYFGEH GDK+WEEIE+ HHSYL+SVKD +
Sbjct: 1   METSTLFCLLLLL--LLPPSSCLAQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKDTE 60

Query: 61  EDAKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTTRSWEFSGVEE 120
            DAK SL+YSYKHTINGFAA+LT Q+A KLSEL+EVV+VIESK Y MHTTRSWEFSGV E
Sbjct: 61  NDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKTYGMHTTRSWEFSGVVE 120

Query: 121 DEPRVHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGICQTGPGFESAH 180
           D+PR+ DLVSKA  GKDVVIGMLDSGVWPKSKSFSD GMGP+PKSWKGICQTGPGFES H
Sbjct: 121 DKPRLSDLVSKAKSGKDVVIGMLDSGVWPKSKSFSDNGMGPVPKSWKGICQTGPGFESPH 180

Query: 181 CNRKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRVHNVSAFGGVA 240
           CNRKIIGARYY+KGYEHHFGRLNET DFRSPCD DGHGSHTAS AGGRRV+NVSAFGGVA
Sbjct: 181 CNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGGVA 240

Query: 241 RGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGVHVLSLSIGKS 300
           RGTASGGAP  RLAIYKVCWAIPNQMK LGNVCFDTD+LAAMDDAI+DGV VLSLSIGKS
Sbjct: 241 RGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIGKS 300

Query: 301 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGASTVDREFYSP 360
           DPYNYTDDGIAIGALHAVKRDIVVSCSAGN GPTPS LSNVAPWIITVGASTVDREFYSP
Sbjct: 301 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFYSP 360

Query: 361 VILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAGSLSHEKTKGK 420
           VILGNGLKIKGLSVAPSKL ++ KMYPLVYAVDIQKPHVP+NESG C+AGSLSH+K KGK
Sbjct: 361 VILGNGLKIKGLSVAPSKL-RRNKMYPLVYAVDIQKPHVPQNESGSCIAGSLSHKKAKGK 420

Query: 421 IVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANLIL 480
           IVLCYRG+GISRYAGSLEVKRSGGAGMILG VPAVG R HADPHFVPATAVSY+DAN IL
Sbjct: 421 IVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRIL 480

Query: 481 KYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITAPGVDILAAWS 540
           KYI SH+ PTATIVPPITIYGSRPAPAMANF+SRGP+L+DPHF+KPDITAPGVDILAAWS
Sbjct: 481 KYIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAPGVDILAAWS 540

Query: 541 EQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAAIRSALMTTST 600
           E+DSPT LPK LD RIVK+N+YSGTSMSCPHV+AAAALLR+IHP+WSQAAIRSALMTTST
Sbjct: 541 EKDSPTXLPKFLDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTST 600

Query: 601 TNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDYLHYLCALNMN 660
           T NK G PITD+S+ D+ S ATPFSFGSGHFRP+ A DPGLVYDANYTDYLHYLC L MN
Sbjct: 601 TTNKYGHPITDDSTSDN-SPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKMN 660

Query: 661 SIDPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERMVTNVGGGGKSVYFFYSRAPPGVA 720
           SID SFKCPPRA HPHDLNYPSIA+PKL+G VRI+R VTNVGGGGKSVYFF S APPGVA
Sbjct: 661 SIDRSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKSVYFFESWAPPGVA 720

Query: 721 VRASPNVLYFDGIGERKMFSIIVSGKVKGKVDASGYAFGWFAWSDGIHYVRSPIAISST 780
           VRASP+VLYFD +G+RK F++ VSGKVKG    SGY+FGWFAWSDGIHYVRSPIAISST
Sbjct: 721 VRASPSVLYFDRVGQRKRFTVTVSGKVKG----SGYSFGWFAWSDGIHYVRSPIAISST 771

BLAST of Sed0024540 vs. ExPASy TrEMBL
Match: A0A0A0KHZ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G520430 PE=3 SV=1)

HSP 1 Score: 1316.6 bits (3406), Expect = 0.0e+00
Identity = 654/793 (82.47%), Postives = 714/793 (90.04%), Query Frame = 0

Query: 1   MEISTFFC--------FLLLLCSLIQQA--SSQNQKQAYIVYFGEHQGDKAWEEIEKIHH 60
           ME S+F C         LLL+   IQQA  SS NQK+AYIVYFGEH G+K+ EEI++ HH
Sbjct: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60

Query: 61  SYLLSVKDNKEDAKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTT 120
           SYL+ VK+++EDAK  L+Y+YKH+IN FAAILTPQ+A KLS+L EVV+VIESKKY M TT
Sbjct: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120

Query: 121 RSWEFSGVEEDEPRVHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGIC 180
           RSWEFSGVEED+P ++DLVS+A +GKDVVIGMLDSGVWPKSKSFSD+GMGPIPKSWKGIC
Sbjct: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180

Query: 181 QTGPGFESAHCNRKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRV 240
           QTGP F+SAHCNRKIIGARYY+KGYEHHFGRLN+T D+RSPCDKDGHGSHTAS AGGRRV
Sbjct: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV 240

Query: 241 HNVSAFGGVARGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGV 300
           +NVSAFGGVA GTASGGAP ARLAIYKVCWAIPNQMK LGNVCFDTDMLAAMDDAIADGV
Sbjct: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300

Query: 301 HVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360
            VLSLSIGKS+PYNYTDDG+AIGALHAVK+DIVVSCSAGNYGPTPSALSNVAPWIITVGA
Sbjct: 301 DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360

Query: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAG 420
           STVDREFYSPVILGNGLKIKGLSVAPSKL ++KKMYPLVYA DI  PH PRN+SGLCVAG
Sbjct: 361 STVDREFYSPVILGNGLKIKGLSVAPSKL-ERKKMYPLVYAGDIMNPHAPRNQSGLCVAG 420

Query: 421 SLSHEKTKGKIVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATA 480
           SLSHEK KGKIVLC+RGEGISR+AGSLEV+RSGGAGMILGNVPAVGRRPHADPHFVPATA
Sbjct: 421 SLSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATA 480

Query: 481 VSYEDANLILKYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITA 540
           VSYEDAN+ILKYI S K PTATIVPP+TIYGSRPAPAMANFSSRGPN IDPHF+KPDITA
Sbjct: 481 VSYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITA 540

Query: 541 PGVDILAAWSEQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAA 600
           PGVDILAAWSEQDSPTKLPK LD RIV++NLYSGTSMSCPHV+AAAALLR+IHP+WSQAA
Sbjct: 541 PGVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAA 600

Query: 601 IRSALMTTSTTNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDY 660
           IRSALMTTSTTNNK GQPITD+S+ D+ S ATPFSFGSGHFRPS A DPGLVYD+NYTDY
Sbjct: 601 IRSALMTTSTTNNKYGQPITDDSTLDN-SPATPFSFGSGHFRPSKAADPGLVYDSNYTDY 660

Query: 661 LHYLCALNMNSIDPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERMVTNVGGGGKSVYF 720
           LHYLC L MNSIDPSFKCPPRA HPHDLNYPSIAVP+LR  VRI+R VTNVGGGGK+VYF
Sbjct: 661 LHYLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYF 720

Query: 721 FYSRAPPGVAVRASPNVLYFDGIGERKMFSIIVSGKVKGKVDAS----GYAFGWFAWSDG 780
           F S AP GVAV ASPN+LYF+ +GERK F+I +S KV     +S     Y+FGWFAWSDG
Sbjct: 721 FKSEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDG 780

BLAST of Sed0024540 vs. ExPASy TrEMBL
Match: A0A1S3B024 (LOW QUALITY PROTEIN: subtilisin-like protease SBT5.6 OS=Cucumis melo OX=3656 GN=LOC103484415 PE=3 SV=1)

HSP 1 Score: 1306.2 bits (3379), Expect = 0.0e+00
Identity = 649/795 (81.64%), Postives = 707/795 (88.93%), Query Frame = 0

Query: 1   MEISTFFC---------FLLLLCSLIQQA--SSQNQKQAYIVYFGEHQGDKAWEEIEKIH 60
           ME S+F C          LLL+   IQQA  SS NQK+AYIVYFGEH G+K+ EEI++ H
Sbjct: 1   MEKSSFLCHCHVLLLLLLLLLIGPFIQQAASSSNNQKKAYIVYFGEHHGEKSVEEIKERH 60

Query: 61  HSYLLSVKDNKEDAKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHT 120
           HSYL+ VK+++EDAK  L+Y+YKH+IN FAAILTPQ+A KLSEL EVV+VIESKKY M T
Sbjct: 61  HSYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMET 120

Query: 121 TRSWEFSGVEEDEPRVHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGI 180
           TRSWEFSGVEED+P ++DLVS+A +GKDVVIGMLDSGVWP SKSFSD+GMGP+PKSWKGI
Sbjct: 121 TRSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPNSKSFSDKGMGPLPKSWKGI 180

Query: 181 CQTGPGFESAHCNRKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRR 240
           CQTGP F+SAHCNRKIIGARYY+KGYEHHFGRLNET D+RSPCDKDGHGSHTAS AGGRR
Sbjct: 181 CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRR 240

Query: 241 VHNVSAFGGVARGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADG 300
           V+NVSAFGGVA GTASGGAP ARLAIYKVCWAIPNQMK LGNVCFDTDMLAAMDDAIADG
Sbjct: 241 VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG 300

Query: 301 VHVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVG 360
           V VLSLSIGKS+PYNYTDDGIAIGALHAVK+DIVVSCSAGNYGPTPSALSNVAPWIITVG
Sbjct: 301 VDVLSLSIGKSEPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVG 360

Query: 361 ASTVDREFYSPVILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVA 420
           ASTVDREFYSPVILGNGLKIKGLSVAPSKL ++KKMYPLVYA DI  PH PRN+SGLCVA
Sbjct: 361 ASTVDREFYSPVILGNGLKIKGLSVAPSKL-ERKKMYPLVYAGDIMNPHAPRNQSGLCVA 420

Query: 421 GSLSHEKTKGKIVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPAT 480
           GSLSHEK KGK VLC+RGEGISR+AGSLEV+RSGGAGMILGNVPAVGRRPHADPHFVPAT
Sbjct: 421 GSLSHEKAKGKXVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPAT 480

Query: 481 AVSYEDANLILKYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDIT 540
           AVSYEDAN+ILKYI S   PTATIVPP+TIYGSRPAPAMANFSSRGPN IDPHF+KPDIT
Sbjct: 481 AVSYEDANVILKYIKSRTNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDIT 540

Query: 541 APGVDILAAWSEQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQA 600
           APGVDILAAWSEQDSPTKL K LD RIV++NLYSGTSMSCPHV+AAAALLR+IHP+WSQA
Sbjct: 541 APGVDILAAWSEQDSPTKLSKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQA 600

Query: 601 AIRSALMTTSTTNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTD 660
           AIRSALMTTSTTNNK GQPITD+S+PD+ S ATPFSFGSGHFRPS A DPGLVYD+NYTD
Sbjct: 601 AIRSALMTTSTTNNKSGQPITDDSTPDN-SPATPFSFGSGHFRPSKAADPGLVYDSNYTD 660

Query: 661 YLHYLCALNMNSIDPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERMVTNVGGGGKSVY 720
           YLHYLC L MNSIDPSF CPPR  HPHDLNYPSIAVP+LR  VRI+R VTNVGGGGKSVY
Sbjct: 661 YLHYLCGLKMNSIDPSFTCPPRPLHPHDLNYPSIAVPQLRDVVRIKRTVTNVGGGGKSVY 720

Query: 721 FFYSRAPPGVAVRASPNVLYFDGIGERKMFSIIVSGKVKGKVDA------SGYAFGWFAW 779
           FF S APPGVAV ASPN+LYF+ +GERK F+I +S KV     +        Y+FGWFAW
Sbjct: 721 FFKSEAPPGVAVSASPNILYFNRVGERKKFTITISRKVNSSSSSRRSKKGEEYSFGWFAW 780

BLAST of Sed0024540 vs. ExPASy TrEMBL
Match: A0A6J1CJX5 (subtilisin-like protease SBT5.6 OS=Momordica charantia OX=3673 GN=LOC111012300 PE=3 SV=1)

HSP 1 Score: 1257.7 bits (3253), Expect = 0.0e+00
Identity = 634/784 (80.87%), Postives = 696/784 (88.78%), Query Frame = 0

Query: 4   STFFCFLLLLCSLI-QQA--SSQNQKQAYIVYFGEHQGDKAWEEIEKIHHSYLLSVKDNK 63
           STF C LLLL  L+ QQA  S  N + AYIVYFGEH+GDKAW EIE  HHSYL SVKD +
Sbjct: 36  STFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSVKDTE 95

Query: 64  EDAKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTTRSWEFSGVEE 123
           E+A+ SLIYSYKH+INGFAA+L+ +EA KLSE+  VV VI S+KYS  TTRSWEFSGVEE
Sbjct: 96  EEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVRVIRSEKYSTQTTRSWEFSGVEE 155

Query: 124 DEPRVHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGICQTGPGFESAH 183
           D+PR++DLVS+A  GKDVVIGMLDSGVWPKSKSFSD+GMGPIPKSW+GICQTGP F+SAH
Sbjct: 156 DKPRLNDLVSRAKFGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWRGICQTGPAFQSAH 215

Query: 184 CNRKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRVHNVSAFGGVA 243
           CNRKIIGARYY+KGYE++FGRLNET D+RSPCDKDGHGSHTASTAGGRRV NVSAFGGVA
Sbjct: 216 CNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAFGGVA 275

Query: 244 RGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGVHVLSLSIGKS 303
           RGTASGGAP ARLAIYKVCWAIP QMKILGNVCFDTD+LAAMDDAIADGV VLSLSIGK+
Sbjct: 276 RGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLSIGKA 335

Query: 304 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGASTVDREFYSP 363
           DPYNYTDDGIAIGALHAVK+DIVVSCSAGNYGPTPSALSN APWIITVGASTVDREFYSP
Sbjct: 336 DPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDREFYSP 395

Query: 364 VILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAGSLSHEKTKGK 423
           VILGNG KI+GLSVAPS L K+KKMYPLVYA DI KPHVP+N+SG+CVAGSLSHEK +GK
Sbjct: 396 VILGNGFKIRGLSVAPSML-KRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKARGK 455

Query: 424 IVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANLIL 483
           IVLCYR EGISRYAGSLEVKRSGGAGMILGNVPAVGR+ HADPHFVPATAVSYEDAN+IL
Sbjct: 456 IVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDANIIL 515

Query: 484 KYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITAPGVDILAAWS 543
           KYI S   PTATIVPPITIYGSRPAPAMANFSSRGPN IDPH +KPDITAPGVDILAAWS
Sbjct: 516 KYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILAAWS 575

Query: 544 EQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAAIRSALMTTST 603
            +DSPTKLPKSLD R+V +NLYSGTSMSCPHVAAAAALLR+IHP+WSQAAIRSALMTT+T
Sbjct: 576 GEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMTTAT 635

Query: 604 TNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDYLHYLCALNM- 663
           T NK G+PITD++S D  + ATPFS+GSGHFRPS A DPGLVYDANYTDYL +LCAL M 
Sbjct: 636 TTNKYGRPITDDAS-DHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALKMA 695

Query: 664 NSIDPSFKCPPRARHPHDLNYPSIAVPKLR----GAVRIERMVTNVGGGGKSVYFFYSRA 723
           +SIDPSF CP RAR  HDLNYPS+AVP+LR    G VR+ R V NV GG KS YFF + A
Sbjct: 696 HSIDPSFTCPARAR-AHDLNYPSVAVPRLRGGGGGGVRVVRTVRNV-GGSKSAYFFRASA 755

Query: 724 PPGVAVRASPNVLYFDGIGERKMFSIIVSGKVK-GKVDASGYAFGWFAWSDGIHYVRSPI 779
           P GV+VRASP+VLYF  +G+RK F+I +SGK + G VD SGY+FGWFAW+DGIH+VRSPI
Sbjct: 756 PAGVSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSPI 815

BLAST of Sed0024540 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 872.1 bits (2252), Expect = 3.4e-253
Identity = 448/792 (56.57%), Postives = 567/792 (71.59%), Query Frame = 0

Query: 10  LLLLCSLIQQASSQNQKQAYIVYFGEHQGDKAWEEIEKIHHSYLLSVKDNKEDAKFSLIY 69
           LL L  L+  AS   +KQ YIVYFGEH+GDKA+ EIE+ HHSYL SVK+++EDA+ SL+Y
Sbjct: 10  LLFLIPLL--ASCAEEKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSVKESEEDARASLLY 69

Query: 70  SYKHTINGFAAILTPQEAFKLSELKEVVTVIES--KKYSMHTTRSWEFSGVEEDE----- 129
           SYKH+INGFAA LTP +A KL +L EVV+V +S  +KY  HTTRSWEF G+EE+E     
Sbjct: 70  SYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDV 129

Query: 130 PR-----------VHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGICQ 189
           PR             + + KA HG  +++G+LDSGVWP+SKSF+D+GMGP+PKSWKGICQ
Sbjct: 130 PRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGICQ 189

Query: 190 TGPGFESAHCNRKIIGARYYIKGYEHHFGRLNETI--DFRSPCDKDGHGSHTASTAGGRR 249
           TG  F S+HCNRKIIGARYY+KGYE ++G  N T   DF SP D DGHGSHTASTA GRR
Sbjct: 190 TGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGRR 249

Query: 250 VHNVSAFGGVARGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADG 309
           V   SA GG A+G+ASGGAP+ARLAIYK CWA PN  K+ GN+C + DMLAA+DDAIADG
Sbjct: 250 VLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADG 309

Query: 310 VHVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVG 369
           VHV+S+SIG ++P+ +T DGIA+GALHAVKR+IVV+ SAGN GP P  LSN+APWIITVG
Sbjct: 310 VHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVG 369

Query: 370 ASTVDREFYSPVILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVA 429
           AST+DR F   ++LGNG  IK  S+   K+    K  PLVYA ++  P +  NE+  C+ 
Sbjct: 370 ASTLDRAFVGGLVLGNGYTIKTDSITAFKM---DKFAPLVYASNVVVPGIALNETSQCLP 429

Query: 430 GSLSHEKTKGKIVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPAT 489
            SL  E   GK+VLC RG G SR    +EVKR+GGAGMILGN+ A G    +D HFVP  
Sbjct: 430 NSLKPELVSGKVVLCLRGAG-SRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPTA 489

Query: 490 AVSYEDANLILKYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDIT 549
            V+    + IL+YI + K P A I P  T+Y  + AP+M  FSSRGPN++DP+ +KPDIT
Sbjct: 490 GVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDIT 549

Query: 550 APGVDILAAWSEQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQA 609
           APG+ ILAAWS  DSP+K+  S+D R+  +N+YSGTSMSCPHVA A ALL++IHP WS A
Sbjct: 550 APGLYILAAWSGADSPSKM--SVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSSA 609

Query: 610 AIRSALMTTSTTNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTD 669
           AIRSALMTT+   N + +PI D +       A PF+ GSGHFRP+ A DPGLVYDA+Y  
Sbjct: 610 AIRSALMTTAWMTNDKKKPIQDTTG----LPANPFALGSGHFRPTKAADPGLVYDASYRA 669

Query: 670 YLHYLCALNMNSIDPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERMVTNVG-GGGKSV 729
           YL Y C++N+ +IDP+FKCP +    ++ NYPSIAVP L+  V ++R VTNVG G   S 
Sbjct: 670 YLLYGCSVNITNIDPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGNSTST 729

Query: 730 YFFYSRAPPGVAVRASPNVLYFDGIGERKMFSIIV---SGKVKGKVDASGYAFGWFAWSD 778
           Y F  + P G++V+A PN+L F+ IG+++ F I++     +V    +   Y FGWF+W+D
Sbjct: 730 YLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWFSWTD 789

BLAST of Sed0024540 vs. TAIR 10
Match: AT5G45640.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 690.3 bits (1780), Expect = 1.8e-198
Identity = 384/745 (51.54%), Postives = 483/745 (64.83%), Query Frame = 0

Query: 74  TINGFAAILTPQEAFKLSELKEVVTVIES--KKYSMHTTRSWEFSGVEEDE--------- 133
           +INGFAA LTP +A +L ELKEVV+V +S  +KY +HTTRSWEF G++E+E         
Sbjct: 37  SINGFAAELTPDQASRLKELKEVVSVFKSDPRKYKIHTTRSWEFVGLKEEEGEDYRSDGD 96

Query: 134 -PR----VHD-------LVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGIC 193
            PR    V+D        +  A HG  V++G++DSGVWP+S+SF D+GMGPIP+SWKGIC
Sbjct: 97  APRHKYDVNDRFRVGRKFLKNAKHGDGVIVGLIDSGVWPESRSFDDKGMGPIPESWKGIC 156

Query: 194 QTGPGFESAHCNRKIIGARYYIKGYEHHFGRLNETI--DFRSPCDKDGHGSHTASTAGGR 253
           QTG  F S+HCN      RYY +GYE ++G  N     DF SP D DGHGSHTASTA GR
Sbjct: 157 QTGVAFNSSHCN------RYYARGYERYYGPFNAEANKDFLSPRDADGHGSHTASTAVGR 216

Query: 254 RVHNVSAFGGVARGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIAD 313
           RV  VSA GG+A GTASGGA +ARLA+YK CWA+PN+ K   N CFD DMLAA DDAIAD
Sbjct: 217 RVDGVSALGGIAMGTASGGASLARLAVYKACWAVPNKEKYATNTCFDEDMLAAFDDAIAD 276

Query: 314 GVHVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITV 373
           GV+V+S+SIG  +P+ Y +DGIAIGALHAVKRDIVV+ SAGN GP    LSN APWIITV
Sbjct: 277 GVNVISISIGTVEPHTYLEDGIAIGALHAVKRDIVVAASAGNDGPARETLSNPAPWIITV 336

Query: 374 GASTVDREFYSPVILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCV 433
           GAS++DR F   + LG+G   +  S+   K+       PLVYA D+  P V RN++ LC+
Sbjct: 337 GASSLDRFFVGRLELGDGYVFESDSLTTLKM---DNYAPLVYAPDVVVPGVSRNDAMLCL 396

Query: 434 AGSLSHEKTKGKIVLCYRGEGISRYAG-SLEVKRSGGAGMILGNVPAVGRRPHADPHFVP 493
             +LS +  +GK+VLC RG G     G  LEVKR+GG GMIL N          + HFVP
Sbjct: 397 PNALSPDHVRGKVVLCLRGYGSGSTIGKGLEVKRAGGVGMILAN-SRDNDAFDVESHFVP 456

Query: 494 ATAVSYEDANLILKYIMSHKKPTATIVPPIT----------IYGSRPAPAMANFSSRGPN 553
              V     + IL YI +  +P A I P  T          +Y  +PAP M +F      
Sbjct: 457 TALVFSSTVDRILDYIYNTYEPVAFIKPAETVLYRNQPEDSVYPYKPAPFMTSF------ 516

Query: 554 LIDPHFIKPDITAPGVDILAAWSEQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAA 613
                   PDI APG++ILAAWS  DS +K   S+D R++ +NL SGTSMSCPHVA A A
Sbjct: 517 -------LPDIIAPGLNILAAWSGADSASK--DSIDRRVLDYNLDSGTSMSCPHVAGAIA 576

Query: 614 LLRSIHPSWSQAAIRSALMTTSTTNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAV 673
           LL+S+HP+WS AAIRSALMTT++  N+  +PI D       S A PF+ GS HFRP+ A 
Sbjct: 577 LLKSMHPTWSSAAIRSALMTTASMTNEDNEPIQDYDG----SPANPFALGSRHFRPTKAA 636

Query: 674 DPGLVYDANYTDYLHYLCALNMNSIDPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERM 733
            PGLVYDA+Y  YL Y C++ + ++DP+FKCP R    ++LNYPSI++P L G V + R 
Sbjct: 637 SPGLVYDASYQSYLLYCCSVGLTNLDPTFKCPSRIPPGYNLNYPSISIPYLSGTVTVTRT 696

Query: 734 VTNVG--GGGKSVYFFYSRAPPGVAVRASPNVLYFDGIGERKMFSIIVSG---KVKGKVD 778
           VT VG  G   SVY F ++ P GV V+A PNVL FD IG++K F+II +    +  G+  
Sbjct: 697 VTCVGRTGNSTSVYVFNAQPPNGVLVKAEPNVLVFDKIGQKKRFNIIFTTQRYEFTGEAR 752

BLAST of Sed0024540 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 620.5 bits (1599), Expect = 1.8e-177
Identity = 364/793 (45.90%), Postives = 485/793 (61.16%), Query Frame = 0

Query: 1   MEISTFFCFLLLLCSLIQQAS----SQNQKQAYIVYFGEHQ-----GDKAWEEIEKIHHS 60
           M+++  F FLLLL  L+  +S    +     +Y+VYFG H       + A + +++ H+ 
Sbjct: 1   MKLTHNFSFLLLLL-LVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYD 60

Query: 61  YLLSVKDNKEDAKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTTR 120
           +L S   ++E A  ++ YSY   INGFAA L    A+++S+  EVV+V  +K   +HTTR
Sbjct: 61  FLGSFTGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTR 120

Query: 121 SWEFSGVEEDE-PRVHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGIC 180
           SW+F G+E +       +  KA  G+D +I  LD+GVWP+SKSF DEG+GPIP  WKGIC
Sbjct: 121 SWDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGIC 180

Query: 181 QTGPGFESAHCNRKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRV 240
           Q      + HCNRK+IGARY+ KGY    G LN + D  SP D DGHGSHT STA G  V
Sbjct: 181 QNQKD-ATFHCNRKLIGARYFNKGYAAAVGHLNSSFD--SPRDLDGHGSHTLSTAAGDFV 240

Query: 241 HNVSAFGGVARGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGV 300
             VS F G   GTA GG+P AR+A YKVCW       + GN C+D D+LAA D AI DG 
Sbjct: 241 PGVSIF-GQGNGTAKGGSPRARVAAYKVCWP-----PVKGNECYDADVLAAFDAAIHDGA 300

Query: 301 HVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360
            V+S+S+G  +P ++ +D +AIG+ HA K+ IVV CSAGN GP  S +SNVAPW ITVGA
Sbjct: 301 DVISVSLG-GEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGA 360

Query: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAG 420
           ST+DREF S ++LGNG   KG S++ + L    K YP++ +V+ +  +    ++ LC  G
Sbjct: 361 STMDREFASNLVLGNGKHYKGQSLSSTAL-PHAKFYPIMASVNAKAKNASALDAQLCKLG 420

Query: 421 SLSHEKTKGKIVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATA 480
           SL   KTKGKI++C RG+   R      V   GG GM+L N    G    ADPH +PAT 
Sbjct: 421 SLDPIKTKGKILVCLRGQN-GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQ 480

Query: 481 VSYEDANLILKYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITA 540
           ++ +D+  + +YI   KKP A I P  T  G +PAP MA+FSS+GP+++ P  +KPDITA
Sbjct: 481 LTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITA 540

Query: 541 PGVDILAAWSEQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAA 600
           PGV ++AA++   SPT   +  D R + FN  SGTSMSCPH++  A LL++ +PSWS AA
Sbjct: 541 PGVSVIAAYTGAVSPTN--EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAA 600

Query: 601 IRSALMTTSTTNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDY 660
           IRSA+MTT+T  +    PI + ++      ATPFSFG+GH +P+ AV+PGLVYD    DY
Sbjct: 601 IRSAIMTTATIMDDIPGPIQNATN----MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDY 660

Query: 661 LHYLCALNMNSIDPS------FKCPPRARHPHDLNYPSIAVPKLRGA-VRIERMVTNVGG 720
           L++LC+L  N+   S      F C        +LNYPSI VP L  + V + R V NV  
Sbjct: 661 LNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNV-- 720

Query: 721 GGKSVYFFYSRAPPGVAVRASPNVLYFDGIGERKMFSIIVSGKVKGKVDASGYAFGWFAW 777
           G  S+Y      P GV V   P  L F  +GE+K F +I+  K KG V A GY FG   W
Sbjct: 721 GRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILV-KSKGNV-AKGYVFGELVW 770

BLAST of Sed0024540 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 599.4 bits (1544), Expect = 4.2e-171
Identity = 351/790 (44.43%), Postives = 466/790 (58.99%), Query Frame = 0

Query: 1   MEISTFFCFLLLLCSLIQQASSQNQKQAYIVYFGEHQ-----GDKAWEEIEKIHHSYLLS 60
           M + +    LLL+      A +   K++YIVY G H           + +   H ++L S
Sbjct: 16  MSLQSLSSLLLLVTLFFSPAFA--LKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLAS 75

Query: 61  VKDNKEDAKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTTRSWEF 120
              + E+AK ++ YSYK  INGFAAIL   EA ++++  +VV+V  +K   +HTT SW F
Sbjct: 76  FVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNF 135

Query: 121 SGVEEDEPRVH--DLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGICQTG 180
             + ++   VH   L +KA +G+D +I  LD+GVWP+SKSFSDEG G +P  WKG C   
Sbjct: 136 MLLAKNGV-VHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH-- 195

Query: 181 PGFESAHCNRKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRVHNV 240
              +   CNRK+IGARY+ KGY  + G L     + +  D DGHGSHT STA G  V   
Sbjct: 196 ---KDVPCNRKLIGARYFNKGYLAYTG-LPSNASYETCRDHDGHGSHTLSTAAGNFVPGA 255

Query: 241 SAFGGVARGTASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGVHVL 300
           + F G+  GTASGG+P AR+A YKVCW       + G  CFD D+LAA++ AI DGV VL
Sbjct: 256 NVF-GIGNGTASGGSPKARVAAYKVCWP-----PVDGAECFDADILAAIEAAIEDGVDVL 315

Query: 301 SLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGASTV 360
           S S+G  D  +Y  DGIAIG+ HAVK  + V CSAGN GP    +SNVAPW+ITVGAS++
Sbjct: 316 SASVG-GDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSM 375

Query: 361 DREFYSPVILGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAGSLS 420
           DREF + V L NG   KG S+  SK   ++KMY L+ A D    +    ++ LC  GSL 
Sbjct: 376 DREFQAFVELKNGQSFKGTSL--SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLD 435

Query: 421 HEKTKGKIVLCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSY 480
            +K KGKI++C RG+  +R    ++   +G AGM+L N  A G    +D H +PA+ + Y
Sbjct: 436 PKKVKGKILVCLRGDN-ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDY 495

Query: 481 EDANLILKYIMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITAPGV 540
           +D   +  Y+ S K P   I  P     ++PAP MA+FSSRGPN I P  +KPDITAPGV
Sbjct: 496 KDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGV 555

Query: 541 DILAAWSEQDSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAAIRS 600
           +I+AA++E   PT L    D+R   FN  SGTSMSCPH++    LL+++HP WS AAIRS
Sbjct: 556 NIIAAFTEATGPTDLDS--DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRS 615

Query: 601 ALMTTSTTNNKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDYLHY 660
           A+MTTS T N R +P+ D    +S   A PFS+GSGH +P+ A  PGLVYD    DYL +
Sbjct: 616 AIMTTSRTRNNRRKPMVD----ESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDF 675

Query: 661 LCALNMNSI-------DPSFKCPPRARHPHDLNYPSIAVPKLRGAVRIERMVTNVGGGGK 720
           LCA+  N+        DP + C   A +  D NYPSI VP L G++ + R + NV  G  
Sbjct: 676 LCAVGYNNTVVQLFAEDPQYTCRQGA-NLLDFNYPSITVPNLTGSITVTRKLKNV--GPP 735

Query: 721 SVYFFYSRAPPGVAVRASPNVLYFDGIGERKMFSIIVSGKVKGKVDASGYAFGWFAWSDG 777
           + Y    R P GV V   P  L F+  GE K+F + +       V  SGY FG   W+D 
Sbjct: 736 ATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPL---PVTPSGYVFGELTWTDS 774

BLAST of Sed0024540 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 589.7 bits (1519), Expect = 3.4e-168
Identity = 351/785 (44.71%), Postives = 475/785 (60.51%), Query Frame = 0

Query: 3   ISTFFCFLLLLCSLIQQASSQNQKQAYIVYFGEHQGDKAWEEIEKIHHSYLLSVKDNKED 62
           +S+   FLLL       +SS + +  YIV+  + Q   +++     + S L S+ D+ E 
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE- 65

Query: 63  AKFSLIYSYKHTINGFAAILTPQEAFKLSELKEVVTVIESKKYSMHTTRSWEFSGVEEDE 122
               L+Y+Y++ I+GF+  LT +EA  L     V++V+   +Y +HTTR+  F G++E  
Sbjct: 66  ----LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE-- 125

Query: 123 PRVHDLVSKAYHGKDVVIGMLDSGVWPKSKSFSDEGMGPIPKSWKGICQTGPGFESAHCN 182
               DL  +A    DVV+G+LD+GVWP+SKS+SDEG GPIP SWKG C+ G  F ++ CN
Sbjct: 126 -HTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCN 185

Query: 183 RKIIGARYYIKGYEHHFGRLNETIDFRSPCDKDGHGSHTASTAGGRRVHNVSAFGGVARG 242
           RK+IGAR++ +GYE   G ++E+ + RSP D DGHG+HT+STA G  V   S   G A G
Sbjct: 186 RKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL-GYASG 245

Query: 243 TASGGAPMARLAIYKVCWAIPNQMKILGNVCFDTDMLAAMDDAIADGVHVLSLSIGKSDP 302
           TA G AP AR+A+YKVCW        LG  CF +D+LAA+D AIAD V+VLS+S+G    
Sbjct: 246 TARGMAPRARVAVYKVCW--------LGG-CFSSDILAAIDKAIADNVNVLSMSLG-GGM 305

Query: 303 YNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNVAPWIITVGASTVDREFYSPVI 362
            +Y  DG+AIGA  A++R I+VSCSAGN GP+ S+LSNVAPWI TVGA T+DR+F +  I
Sbjct: 306 SDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAI 365

Query: 363 LGNGLKIKGLSVAPSKLKKKKKMYPLVYAVDIQKPHVPRNESGLCVAGSLSHEKTKGKIV 422
           LGNG    G+S+   +     K+ P +YA +            LC+ G+L  EK KGKIV
Sbjct: 366 LGNGKNFTGVSLFKGE-ALPDKLLPFIYAGNASN----ATNGNLCMTGTLIPEKVKGKIV 425

Query: 423 LCYRGEGISRYAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANLILKY 482
           +C RG       G + VK +GG GMIL N  A G    AD H +PAT V  +  ++I  Y
Sbjct: 426 MCDRGINARVQKGDV-VKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHY 485

Query: 483 IMSHKKPTATIVPPITIYGSRPAPAMANFSSRGPNLIDPHFIKPDITAPGVDILAAWSEQ 542
           + +   PTA+I    T+ G +P+P +A FSSRGPN I P+ +KPD+ APGV+ILAAW+  
Sbjct: 486 VTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGA 545

Query: 543 DSPTKLPKSLDSRIVKFNLYSGTSMSCPHVAAAAALLRSIHPSWSQAAIRSALMTTSTTN 602
             PT L  + DSR V+FN+ SGTSMSCPHV+  AALL+S+HP WS AAIRSALMTT+   
Sbjct: 546 AGPTGL--ASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKT 605

Query: 603 NKRGQPITDNSSPDSLSHATPFSFGSGHFRPSHAVDPGLVYDANYTDYLHYLCALNMNSI 662
            K G+P+ D ++      +TPF  G+GH  P+ A +PGL+YD    DYL +LCALN  S 
Sbjct: 606 YKDGKPLLDIATG---KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSP 665

Query: 663 D------PSFKC-PPRARHPHDLNYPSIAV-PKLRGAVRIERMVTNVGGGGKSVYFFYSR 722
                   ++ C P ++    DLNYPS AV     GA +  R VT+VGG G       S 
Sbjct: 666 QIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSE 725

Query: 723 APPGVAVRASPNVLYFDGIGERKMFSIIVSGKVKGKVDASGYAFGWFAWSDGIHYVRSPI 780
              GV +   P VL F    E+K +++  +  V     +   +FG   WSDG H V SP+
Sbjct: 726 T-TGVKISVEPAVLNFKEANEKKSYTVTFT--VDSSKPSGSNSFGSIEWSDGKHVVGSPV 757