Sed0024477 (gene) Chayote v1

Overview
NameSed0024477
Typegene
OrganismSechium edule (Chayote v1)
DescriptionProtein-L-isoaspartate O-methyltransferase
LocationLG12: 34430955 .. 34434532 (-)
RNA-Seq ExpressionSed0024477
SyntenySed0024477
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTTTCAGAGAGAGAAATGAATGTTACTCAAATATCATAATTCCCATTAGCATATGTGCTGCCACCACTCTGTGCTTCCTCTCCGCCGTCGTCGCCGCCGTCTCTCCGCCCTAACTCCGACCACCTCGTCTCCTAATTACCTCGGATTCTCACCTTCTTTCTCTTCTTTCAGAGATTTACGGTATCTGAAATTGGAGATTCTCGGATATACCAAAATGGATAAAATGCCCGCCCAGCCATTTTCGTCGCTCAAGGTATTTCGTGCTTGCGTGTTCATCGGTAGCCGAATTCGTTTTTTCATTTTTATGTTTTTGTGTTGAATTTTCACCTAGACATGGGAGAAGGCTGTGATTATCATCAACTGGAATATTCGCTTAATCGGAGTTCGTAATTCGTCTTTCTCCGTTCTTTGGATTGTTAGTAGCTCTTTCGATCTGATGTATATGTTCTTTTGCTAGTGAATATTTGTCGTTTAGTCCTCTCTTAGGATGCGAATTTTTTCTGTAGAGTGCTGGATTATCCCTTTGAAACTGCTTGATGAATTCTTAGGTCTCGTAAGTTCAATCATTCAATATATCTGTTTCAATACATGTAGTTTCAGGGGGTTTTGTTTCGAGTGGTGTGGCTTGTGTGTTTTATGCTGGTACATATATTTTCCCAGGGATAAGCAATCTTTTGATTATGATATTGCATACATGTCCGAGTGTATGCCCCTATAAGAATGAAGAACGAACTAAGCATCAATTTGAAGATAGATAGATTTACAAATTAATGCGTAGAGATGGGAGATAGCTTTAGAAACTTGTTACTTGGTTGATATAATTAAATTTACCCCAACCCATCAGCTTAGACTTTTGGATTGATTGGTGGTTTAACATAATTCAACAAAACTCCTTGTGAATTGTGCTCTGGTGTCGTTTTCGTTTCTTTGTTTTTGTTGAAAGTTTGGTTGCTCCATGTCGTTTTTTATCATTGCAGAGGTTCTGGACTGGAAGTGGCATAGGTACGAACAAGGACATGGTGGAGAATCTCCAGAAATGTGGAGTGGTTAGCTCGAAGAAGGTGTTCGAAGTAATGGAGAGTATAGATAGGGCCTTTTTTGCGCCGAATGGTACTTCACCTTATGTTGATACTCCAGTGCCAATAGGCTACAATGTCACTATATCTGCACCTCACATGCATGCTACTTGCCTTCAATTGTTGGAGAAACACTTGCAACCTGGAATGCACGCTTTGGACGTTGGCTCAGGTCTTCTAACTAACATCTTCTATGTCCTAAAAAATCAATTGATGATTTTATCATTCTTGTTGAATTCTTAAGAACGCTCAATTATGTTTTCTTTTGAAACCAATGCTCAATTATTTTTGAGTTCCATCTCCGAGGGTGGCGCAGTGGTTGAAAACTTGGGATTTGAAGGTGTGCTCCCCTCAAGGTCCTAGGTTTGAGACTCAACTATGACATTAATTTGTTGAGATCTCCCTTGTCTCCAATCCATTCCTATGATTTATACTGGTGCCGCAACCAAGGGATGAGCCAGTGGTGCCTCCGGGTTTAGAGGAGCATAAGCCCTAAGTTACCGGGTCATAAAAAAAATGTTTTAGTTTTAAATAACTTAAAATACATTCTGGTTCTGAATTTCTAATCAGCAACCCCATCTTTCTGGTCTGTCCTATGAGGGTTCAGTGTACTCTAGTCTAAACATAGTCATACACTGCCCATGTGATAGTCAAGTTGATTGTTAATGGATGTAGTCTTCATTGTTCAAGTTGCTATATTTTCAACTATTTGGTTTCCCGACTTGCCGCAATATGCTAAAAAAAAAACCCAACAAACTTCCTCCTACAGTTTGTTTTTCTAGGGTTGTGCAACTGTTACACTAAGAAGATACAATTTCCTTACCTTTTAGCATTTGAAATTTGCATTCCAAAAATGTCATATCCTTGACCTGCCTCGGAGTTCCTATAATATTTGAGTTGAGTTTACACATTTGTTCTCCTTGGCAGGAACGGGATATTTGACAGCATGCTTTGCGTTAATGGTTGGACCTGAAGGTCGTGCTGTTGGTGTGGAACATATTCCTGAGCTCGTTGCTTCTTCAATCGAGAATATTAATAAAAGTGCTGCTGATCCTTTACTGAAAGAAGGTTCCCTATCTGTACATGTTGGTGGTATGTTTGTCTTTTTTCCCTTTTCTAGAGCCATAGATTCTTTTGATCTAAGATTAAAGAACACTGTTCCTTAGTTCAGTCGATTTTAAGAAAAATGTAGTGAAGTGATCGTTAAGTAATTATCTCGATCCCCCTTTTTGAGTGCTTGCAATTTTTTAAATAAGAAAATCAAGGTACTTTCGGCTAGATACTAAATGCCGTGAGTAAAATCCTTAAATTTAGACATTGTACTTTTTACTACCACAAAAGAGTCATGCTAAAGCCATCTAAAACGAGATTTCTTCTCTTCGAGATTGAGCAGAGGAGAAAGTTGAAGAGTTCAATGGAATCTATAGTTCAATTCTTCGAGATTGAGCAGAGGAGAAAAATAGGATGAGCTGCGAGTGTGTGTGAGTGTTTGGGATCTTACAACATTTGAGTGTCAAAGAAACCCTAAAAAAAGGTGGTTACCGTGAATTAAACTTTTAGGTTGCCCAAAGCCATCTTACCACTAAGGGGGTATTTGGGGCTAAGAGGAAAATGAGTACGTGTGTCATTATTTGTGTCACTGAGAATATTCACTCTATAATTGCATTTCTTTAAAATCACTTTTTTTTTCCTCAACTACTTTTTGTTTTCACTATTTTTCACTCTCATCATATTCTTTGTATTTTTTTTTCTTCAAACACAAATAATGATTTTCTCTATAAAATTATTGTCTTTACCTTAAATACAAATAATCATTCTCCTCCTAATTCTCATTCTCCTTAGCTCAAATACCCCTAAAGCCAATTCATGATGGTTGTATTTGTTCTATATAAATATAATGTGTTGGAAATGGAAGTTTACTGTCAATTGGTTTTCGATGTTGTAGATGGCAGGCAGGGCTGGGCAGAGTGTGCGCCTTATGATGCCATTCATGTTGGGGCAGCGGCAGCTGAGATTCCGGCAGCTCTGATTGAGCAGTTAAAGCCAGGGGGGAGAATGGTGATTCCAGTTGGGAACATATTCCAGGATTTGAAGGTTGTGGACAAAGATGTTGATGGCTCTGTTACCATCCATGATGAGATTTCTGTTCGTTATGTGCCTCTCACCAGTCGAGAAGCTCAGTTACGTGACGACTGATCTCGTTTGGTAATCATTCTCAGGTGTTGTATTTATGATGAATGTTGTATAGAAACTTCTCGAAACCATTTTCGTCGAATTGAATCTTATTGTCCTTGTAAATTCTTCCTCAATGTGGATACTTGTCCAAATAAGGTTGTTGTACCCTTTGGTTTCTTCAATCAGCATACTACATTAAGATTACTCGTTTAAAAACTTGCGAAACGGCCCTCTTTGATCAACCTCTAGAGGGGGCTTGATATTTCATTGGTTTCTTTGGTATTTTTGTAGTTTGTACTACTGCATCATATTGAATACATTAGAG

mRNA sequence

TATTTTCAGAGAGAGAAATGAATGTTACTCAAATATCATAATTCCCATTAGCATATGTGCTGCCACCACTCTGTGCTTCCTCTCCGCCGTCGTCGCCGCCGTCTCTCCGCCCTAACTCCGACCACCTCGTCTCCTAATTACCTCGGATTCTCACCTTCTTTCTCTTCTTTCAGAGATTTACGGTATCTGAAATTGGAGATTCTCGGATATACCAAAATGGATAAAATGCCCGCCCAGCCATTTTCGTCGCTCAAGAGGTTCTGGACTGGAAGTGGCATAGGTACGAACAAGGACATGGTGGAGAATCTCCAGAAATGTGGAGTGGTTAGCTCGAAGAAGGTGTTCGAAGTAATGGAGAGTATAGATAGGGCCTTTTTTGCGCCGAATGGTACTTCACCTTATGTTGATACTCCAGTGCCAATAGGCTACAATGTCACTATATCTGCACCTCACATGCATGCTACTTGCCTTCAATTGTTGGAGAAACACTTGCAACCTGGAATGCACGCTTTGGACGTTGGCTCAGGAACGGGATATTTGACAGCATGCTTTGCGTTAATGGTTGGACCTGAAGGTCGTGCTGTTGGTGTGGAACATATTCCTGAGCTCGTTGCTTCTTCAATCGAGAATATTAATAAAAGTGCTGCTGATCCTTTACTGAAAGAAGGTTCCCTATCTGTACATGTTGGTGATGGCAGGCAGGGCTGGGCAGAGTGTGCGCCTTATGATGCCATTCATGTTGGGGCAGCGGCAGCTGAGATTCCGGCAGCTCTGATTGAGCAGTTAAAGCCAGGGGGGAGAATGGTGATTCCAGTTGGGAACATATTCCAGGATTTGAAGGTTGTGGACAAAGATGTTGATGGCTCTGTTACCATCCATGATGAGATTTCTGTTCGTTATGTGCCTCTCACCAGTCGAGAAGCTCAGTTACGTGACGACTGATCTCGTTTGGTAATCATTCTCAGGTGTTGTATTTATGATGAATGTTGTATAGAAACTTCTCGAAACCATTTTCGTCGAATTGAATCTTATTGTCCTTGTAAATTCTTCCTCAATGTGGATACTTGTCCAAATAAGGTTGTTGTACCCTTTGGTTTCTTCAATCAGCATACTACATTAAGATTACTCGTTTAAAAACTTGCGAAACGGCCCTCTTTGATCAACCTCTAGAGGGGGCTTGATATTTCATTGGTTTCTTTGGTATTTTTGTAGTTTGTACTACTGCATCATATTGAATACATTAGAG

Coding sequence (CDS)

ATGTGCTGCCACCACTCTGTGCTTCCTCTCCGCCGTCGTCGCCGCCGTCTCTCCGCCCTAACTCCGACCACCTCGTCTCCTAATTACCTCGGATTCTCACCTTCTTTCTCTTCTTTCAGAGATTTACGGTATCTGAAATTGGAGATTCTCGGATATACCAAAATGGATAAAATGCCCGCCCAGCCATTTTCGTCGCTCAAGAGGTTCTGGACTGGAAGTGGCATAGGTACGAACAAGGACATGGTGGAGAATCTCCAGAAATGTGGAGTGGTTAGCTCGAAGAAGGTGTTCGAAGTAATGGAGAGTATAGATAGGGCCTTTTTTGCGCCGAATGGTACTTCACCTTATGTTGATACTCCAGTGCCAATAGGCTACAATGTCACTATATCTGCACCTCACATGCATGCTACTTGCCTTCAATTGTTGGAGAAACACTTGCAACCTGGAATGCACGCTTTGGACGTTGGCTCAGGAACGGGATATTTGACAGCATGCTTTGCGTTAATGGTTGGACCTGAAGGTCGTGCTGTTGGTGTGGAACATATTCCTGAGCTCGTTGCTTCTTCAATCGAGAATATTAATAAAAGTGCTGCTGATCCTTTACTGAAAGAAGGTTCCCTATCTGTACATGTTGGTGATGGCAGGCAGGGCTGGGCAGAGTGTGCGCCTTATGATGCCATTCATGTTGGGGCAGCGGCAGCTGAGATTCCGGCAGCTCTGATTGAGCAGTTAAAGCCAGGGGGGAGAATGGTGATTCCAGTTGGGAACATATTCCAGGATTTGAAGGTTGTGGACAAAGATGTTGATGGCTCTGTTACCATCCATGATGAGATTTCTGTTCGTTATGTGCCTCTCACCAGTCGAGAAGCTCAGTTACGTGACGACTGA

Protein sequence

MCCHHSVLPLRRRRRRLSALTPTTSSPNYLGFSPSFSSFRDLRYLKLEILGYTKMDKMPAQPFSSLKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTSPYVDTPVPIGYNVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPEGRAVGVEHIPELVASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPAALIEQLKPGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQLRDD
Homology
BLAST of Sed0024477 vs. NCBI nr
Match: XP_016903206.1 (PREDICTED: protein-L-isoaspartate O-methyltransferase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 455.3 bits (1170), Expect = 4.0e-124
Identity = 237/306 (77.45%), Postives = 254/306 (83.01%), Query Frame = 0

Query: 1   MCCHHSVLPLRRRRRRL-SALTPTTSS------PNYLGFSPSFSSFRDLR----YLKLEI 60
           + CHH     RRRRRRL +AL PTTSS         + F P F     L       ++EI
Sbjct: 31  LSCHH-----RRRRRRLPTALPPTTSSVPKTTVTTIISFLPRFLDPHFLTGNCLLSRMEI 90

Query: 61  LGYTKMDKMPAQPFSSLKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFA 120
           LG+ KMDK+P + F++LKRFWTGSGIGTNKDMVE LQ  GVV SKKV EVME+IDRAFF 
Sbjct: 91  LGFLKMDKLPPRSFTTLKRFWTGSGIGTNKDMVEKLQTYGVVRSKKVSEVMENIDRAFFV 150

Query: 121 PNGTSPYVDTPVPIGYNVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALM 180
           P+   PYVDTPVPIGYN TISAPHMHATCLQLLEKHLQPGM ALDVGSGTGYLTACFALM
Sbjct: 151 PDDVPPYVDTPVPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALM 210

Query: 181 VGPEGRAVGVEHIPELVASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHV 240
           VGPEGR VGVEHIPELVASS+ENI KSAA PLLKEGSLS+HVGDGRQGWAECAPYDAIHV
Sbjct: 211 VGPEGRVVGVEHIPELVASSMENIKKSAAAPLLKEGSLSLHVGDGRQGWAECAPYDAIHV 270

Query: 241 GAAAAEIPAALIEQLKPGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSRE 296
           GAAAAEIP ALI+QLKPGGRMVIPVGNIFQDLKVVDKD DGSV+IHDE SVRYVPLTSRE
Sbjct: 271 GAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDSDGSVSIHDETSVRYVPLTSRE 330

BLAST of Sed0024477 vs. NCBI nr
Match: KAA0057806.1 (protein-L-isoaspartate O-methyltransferase 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 453.4 bits (1165), Expect = 1.5e-123
Identity = 236/306 (77.12%), Postives = 253/306 (82.68%), Query Frame = 0

Query: 1   MCCHHSVLPLRRRRRRL-SALTPTTSS------PNYLGFSPSFSSFRDLR----YLKLEI 60
           + CHH     RRRRRRL +AL PTTSS         + F P F     L       ++EI
Sbjct: 31  LSCHHH---RRRRRRRLPTALPPTTSSVPKTTVTTIISFLPRFLDPHFLTGNCLLSRMEI 90

Query: 61  LGYTKMDKMPAQPFSSLKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFA 120
           LG+ KMDK+P + F++LKRFWTGSGIGTNKDMVE LQ  GVV SKKV EVME+IDRAFF 
Sbjct: 91  LGFLKMDKLPPRSFTTLKRFWTGSGIGTNKDMVEKLQTYGVVRSKKVSEVMENIDRAFFV 150

Query: 121 PNGTSPYVDTPVPIGYNVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALM 180
           P+   PYVDTPVPIGYN TISAPHMHATCLQLLEKHLQPGM ALDVGSGTGYLTACFALM
Sbjct: 151 PDDVPPYVDTPVPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALM 210

Query: 181 VGPEGRAVGVEHIPELVASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHV 240
           VGPEGR VGVEHIPELVASS+ENI KSAA PLLKEGSLS+HVGDGRQGWAECAPYDAIHV
Sbjct: 211 VGPEGRVVGVEHIPELVASSMENIKKSAAAPLLKEGSLSLHVGDGRQGWAECAPYDAIHV 270

Query: 241 GAAAAEIPAALIEQLKPGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSRE 296
           GAAA EIP ALI+QLKPGGRMVIPVGNIFQDLKVVDKD DGSV+IHDE SVRYVPLTSRE
Sbjct: 271 GAAATEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDSDGSVSIHDETSVRYVPLTSRE 330

BLAST of Sed0024477 vs. NCBI nr
Match: XP_023515897.1 (protein-L-isoaspartate O-methyltransferase 1-like isoform X5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 435.6 bits (1119), Expect = 3.3e-118
Identity = 212/246 (86.18%), Postives = 225/246 (91.46%), Query Frame = 0

Query: 50  LGYTKMDKMPAQPFSSLKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFA 109
           + + KMD++P  PF +LKRFW+GSGIGTNK+MVENLQ  GVV SKKV EVMES+DRAFF 
Sbjct: 1   MDFPKMDELPPHPFLTLKRFWSGSGIGTNKEMVENLQNYGVVRSKKVSEVMESVDRAFFV 60

Query: 110 PNGTSPYVDTPVPIGYNVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALM 169
           P+G  PYVDTPVPIGYN TISAPHMHATCLQLLEKHLQPGM ALDVGSGTGYLTACFALM
Sbjct: 61  PDGVPPYVDTPVPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALM 120

Query: 170 VGPEGRAVGVEHIPELVASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHV 229
           VGPEGRAVGVEHIPELVASSI+NI KSAA PLLKEGSLSVHVGDGRQGWAECAPYDAIHV
Sbjct: 121 VGPEGRAVGVEHIPELVASSIKNIEKSAAAPLLKEGSLSVHVGDGRQGWAECAPYDAIHV 180

Query: 230 GAAAAEIPAALIEQLKPGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSRE 289
           GAAAAEIP ALI+QLKPGGRMVIPVGNIFQDLKVVDKD DGS+ IHDE SVRYVPLTSRE
Sbjct: 181 GAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLTSRE 240

Query: 290 AQLRDD 296
           AQL D+
Sbjct: 241 AQLGDN 246

BLAST of Sed0024477 vs. NCBI nr
Match: XP_031736272.1 (protein-L-isoaspartate O-methyltransferase 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 429.9 bits (1104), Expect = 1.8e-116
Identity = 210/241 (87.14%), Postives = 222/241 (92.12%), Query Frame = 0

Query: 55  MDKMPAQPFSSLKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTS 114
           MDK+P + F++LKRFW+GSGIGTNKDMVE LQ  GVV SK+V EVMESIDRAFF P+   
Sbjct: 1   MDKLPPRSFTTLKRFWSGSGIGTNKDMVEKLQTYGVVRSKRVSEVMESIDRAFFVPDDVP 60

Query: 115 PYVDTPVPIGYNVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPEG 174
           PYVDTPVPIGYN TISAPHMHATCLQLLEKHLQPGM ALDVGSGTGYLTACFALMVGPEG
Sbjct: 61  PYVDTPVPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEG 120

Query: 175 RAVGVEHIPELVASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAA 234
           R VGVEHIPELVASS+ENI KSAA PLLKEGSLS+HVGDGRQGWAECAPYDAIHVGAAAA
Sbjct: 121 RVVGVEHIPELVASSMENIKKSAAAPLLKEGSLSLHVGDGRQGWAECAPYDAIHVGAAAA 180

Query: 235 EIPAALIEQLKPGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQLRD 294
           EIP ALI+QLKPGGRMVIPVGN+FQDLKVVDKD DGSV+IHDE SVRYVPLTSREAQLRD
Sbjct: 181 EIPPALIDQLKPGGRMVIPVGNVFQDLKVVDKDSDGSVSIHDETSVRYVPLTSREAQLRD 240

Query: 295 D 296
           D
Sbjct: 241 D 241

BLAST of Sed0024477 vs. NCBI nr
Match: XP_022987123.1 (protein-L-isoaspartate O-methyltransferase 1-like isoform X5 [Cucurbita maxima])

HSP 1 Score: 429.9 bits (1104), Expect = 1.8e-116
Identity = 209/241 (86.72%), Postives = 222/241 (92.12%), Query Frame = 0

Query: 55  MDKMPAQPFSSLKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTS 114
           MD++P  PF +LKRFW+GSGIGTNK+MVENLQ  GVV SKKV EVMES+DRAFF P+G  
Sbjct: 1   MDELPPHPFLTLKRFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPDGVP 60

Query: 115 PYVDTPVPIGYNVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPEG 174
           PYVDTP+PIGYN TISAPHMHATCLQLLEKHLQPGM ALDVGSGTGYLTACFALMVGPEG
Sbjct: 61  PYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEG 120

Query: 175 RAVGVEHIPELVASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAA 234
           RAVGVEHIPELVASSI+NI KSAA PLLK+GSLSVHVGDGRQGWAECAPYDAIHVGAAAA
Sbjct: 121 RAVGVEHIPELVASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGAAAA 180

Query: 235 EIPAALIEQLKPGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQLRD 294
           EIP ALI+QLKPGGRMVIPVGNIFQDLKVVDKD DGS+ IHDE SVRYVPLTSREAQL D
Sbjct: 181 EIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQLGD 240

Query: 295 D 296
           +
Sbjct: 241 N 241

BLAST of Sed0024477 vs. ExPASy Swiss-Prot
Match: Q42539 (Protein-L-isoaspartate O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PIMT1 PE=1 SV=3)

HSP 1 Score: 355.1 bits (910), Expect = 7.4e-97
Identity = 174/230 (75.65%), Postives = 198/230 (86.09%), Query Frame = 0

Query: 66  LKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTSPYVDTPVPIGY 125
           +K+FW+ S I  NK MVENLQ  G+V+S +V + ME++DR  F  + +S YVD+P+ IGY
Sbjct: 1   MKQFWSPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGY 60

Query: 126 NVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPEGRAVGVEHIPEL 185
           NVTISAPHMHA CLQLLEKHL+PGM  LDVGSGTGYLTACFA+MVG EGRA+GVEHIPEL
Sbjct: 61  NVTISAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPEL 120

Query: 186 VASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPAALIEQLK 245
           VASS++NI  SAA P LKEGSL+VHVGDGRQGWAE APYDAIHVGAAA EIP ALI+QLK
Sbjct: 121 VASSVKNIEASAASPFLKEGSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLK 180

Query: 246 PGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQLRDD 296
           PGGR+VIPVGNIFQDL+VVDK+ DGSV+I DE SVRYVPLTSREAQLR D
Sbjct: 181 PGGRLVIPVGNIFQDLQVVDKNSDGSVSIKDETSVRYVPLTSREAQLRGD 230

BLAST of Sed0024477 vs. ExPASy Swiss-Prot
Match: Q64J17 (Protein-L-isoaspartate O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PIMT2 PE=1 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.0e-89
Identity = 171/291 (58.76%), Postives = 213/291 (73.20%), Query Frame = 0

Query: 10  LRRRRRRLSA---LTPTTSSPNYLGFSPS-----FSSFRDLRYLKLEILGYTKMDKMPAQ 69
           +++R++++ A   L PT ++   L  +P       SS    ++L L       + +    
Sbjct: 22  IKKRKKKMRAQVLLCPTITAYGRLYCAPRRLHRYNSSSSSSQFLNL------NLSRFSGA 81

Query: 70  PFSSLKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTSPYVDTPV 129
            F  +++F +G+G    + MVENL++ GV+SSK+V +VME++DR  F P G+S YVDTPV
Sbjct: 82  LFFHMEQFQSGTGSSGKRGMVENLKRYGVISSKRVAQVMEALDRGLFVPVGSSAYVDTPV 141

Query: 130 PIGYNVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPEGRAVGVEH 189
           PIGYN TISAPHMHATCLQLLE  L PGM ALDVGSGTGYLT CFALMVG EGR VGV+H
Sbjct: 142 PIGYNATISAPHMHATCLQLLEDKLHPGMRALDVGSGTGYLTGCFALMVGAEGRVVGVDH 201

Query: 190 IPELVASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPAALI 249
           IPELV  SI+NI KS A   LK+GSLS+HVGDGR+GW E APYDAIHVGAAA+EIP  L+
Sbjct: 202 IPELVDMSIKNIEKSVAASFLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAAASEIPQPLL 261

Query: 250 EQLKPGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQL 293
           +QLKPGGRMVIP+G  FQ+LKV+DK+ DGS+ +H E SVRYVPLTSR  QL
Sbjct: 262 DQLKPGGRMVIPLGTYFQELKVIDKNEDGSIKVHTETSVRYVPLTSRVEQL 306

BLAST of Sed0024477 vs. ExPASy Swiss-Prot
Match: Q43209 (Protein-L-isoaspartate O-methyltransferase OS=Triticum aestivum OX=4565 GN=PCM PE=1 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.3e-88
Identity = 155/229 (67.69%), Postives = 186/229 (81.22%), Query Frame = 0

Query: 66  LKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTSPYVDTPVPIGY 125
           + +FW    +  N  +VE L++ GVV + KV EVME+IDRA F P G +PY D+P+PIGY
Sbjct: 1   MAQFWAEGSLEKNNALVEYLKQYGVVRTDKVAEVMETIDRALFVPEGFTPYTDSPMPIGY 60

Query: 126 NVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPEGRAVGVEHIPEL 185
           N TISAPHMHATCL+LL+ +LQPGMHALDVGSG+GYLTACFA+MVGPEGRAVG+EHIPEL
Sbjct: 61  NATISAPHMHATCLELLKDYLQPGMHALDVGSGSGYLTACFAMMVGPEGRAVGIEHIPEL 120

Query: 186 VASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPAALIEQLK 245
           V +S EN+ +SAA  L+K+GSLS HV DGR GW + APYDAIHVGAAA EIP  L+EQLK
Sbjct: 121 VVASTENVERSAAAALMKDGSLSFHVSDGRLGWPDAAPYDAIHVGAAAPEIPRPLLEQLK 180

Query: 246 PGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQLRD 295
           PGGRMVIPVG   QDL+V+DK  DGS ++ ++ SVRYVPLTSR AQL+D
Sbjct: 181 PGGRMVIPVGTYSQDLQVIDKSADGSTSVRNDASVRYVPLTSRSAQLQD 229

BLAST of Sed0024477 vs. ExPASy Swiss-Prot
Match: Q5F3N1 (Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Gallus gallus OX=9031 GN=PCMT1 PE=2 SV=3)

HSP 1 Score: 221.9 bits (564), Expect = 9.8e-57
Identity = 121/226 (53.54%), Postives = 155/226 (68.58%), Query Frame = 0

Query: 73  SGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTSPYVDTPVPIGYNVTISAP 132
           SG  ++ +++ NL+K G++ S KVFEVM + DR  +A    +PY+D+P  IG+  TISAP
Sbjct: 5   SGGASHSELIHNLRKNGIIKSDKVFEVMLATDRCHYAK--YNPYMDSPQSIGFQATISAP 64

Query: 133 HMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPEGRAVGVEHIPELVASSIEN 192
           HMHA  L+LL   L  G  ALDVGSG+G LTACF+ MVGP+G+ VG++HI ELV  SI N
Sbjct: 65  HMHAYALELLSDQLHEGAKALDVGSGSGILTACFSRMVGPKGQVVGIDHIKELVDDSINN 124

Query: 193 INKSAADP-LLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPAALIEQLKPGGRMV 252
           + K   DP LL  G + + VGDGR G+AE APYDAIHVGAAA  +P ALI+QLKPGGR++
Sbjct: 125 VKKD--DPTLLSSGRVKLIVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLI 184

Query: 253 IPVGNI--FQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQLRDD 296
           +PVG     Q L+  DK  DGSV +   + V YVPLT +E Q   D
Sbjct: 185 LPVGPAGGNQMLEQYDKLEDGSVKMKPLMGVIYVPLTDKEKQWSRD 226

BLAST of Sed0024477 vs. ExPASy Swiss-Prot
Match: P80895 (Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Sus scrofa OX=9823 GN=PCMT1 PE=1 SV=3)

HSP 1 Score: 221.1 bits (562), Expect = 1.7e-56
Identity = 119/222 (53.60%), Postives = 154/222 (69.37%), Query Frame = 0

Query: 73  SGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTSPYVDTPVPIGYNVTISAP 132
           SG  ++ +++ NL+K G++ + KVFEVM + DR+ +A    +PY+D+P  IG+  TISAP
Sbjct: 5   SGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAK--CNPYMDSPQSIGFQATISAP 64

Query: 133 HMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPEGRAVGVEHIPELVASSIEN 192
           HMHA  L+LL   L  G  ALDVGSG+G LTACFA MVGP G+ +G++HI ELV  SI N
Sbjct: 65  HMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGPSGKVIGIDHIKELVDDSINN 124

Query: 193 INKSAADP-LLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPAALIEQLKPGGRMV 252
           + K   DP LL  G + + VGDGR G+AE APYDAIHVGAAA  +P ALI+QLKPGGR++
Sbjct: 125 VRKD--DPMLLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLI 184

Query: 253 IPVGNI--FQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQ 292
           +PVG     Q L+  DK  DGSV +   + V YVPLT +E Q
Sbjct: 185 LPVGPAGGNQMLEQYDKLQDGSVKMKPLMGVIYVPLTDKEKQ 222

BLAST of Sed0024477 vs. ExPASy TrEMBL
Match: A0A1S4E4P7 (protein-L-isoaspartate O-methyltransferase 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502358 PE=3 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.9e-124
Identity = 237/306 (77.45%), Postives = 254/306 (83.01%), Query Frame = 0

Query: 1   MCCHHSVLPLRRRRRRL-SALTPTTSS------PNYLGFSPSFSSFRDLR----YLKLEI 60
           + CHH     RRRRRRL +AL PTTSS         + F P F     L       ++EI
Sbjct: 31  LSCHH-----RRRRRRLPTALPPTTSSVPKTTVTTIISFLPRFLDPHFLTGNCLLSRMEI 90

Query: 61  LGYTKMDKMPAQPFSSLKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFA 120
           LG+ KMDK+P + F++LKRFWTGSGIGTNKDMVE LQ  GVV SKKV EVME+IDRAFF 
Sbjct: 91  LGFLKMDKLPPRSFTTLKRFWTGSGIGTNKDMVEKLQTYGVVRSKKVSEVMENIDRAFFV 150

Query: 121 PNGTSPYVDTPVPIGYNVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALM 180
           P+   PYVDTPVPIGYN TISAPHMHATCLQLLEKHLQPGM ALDVGSGTGYLTACFALM
Sbjct: 151 PDDVPPYVDTPVPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALM 210

Query: 181 VGPEGRAVGVEHIPELVASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHV 240
           VGPEGR VGVEHIPELVASS+ENI KSAA PLLKEGSLS+HVGDGRQGWAECAPYDAIHV
Sbjct: 211 VGPEGRVVGVEHIPELVASSMENIKKSAAAPLLKEGSLSLHVGDGRQGWAECAPYDAIHV 270

Query: 241 GAAAAEIPAALIEQLKPGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSRE 296
           GAAAAEIP ALI+QLKPGGRMVIPVGNIFQDLKVVDKD DGSV+IHDE SVRYVPLTSRE
Sbjct: 271 GAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDSDGSVSIHDETSVRYVPLTSRE 330

BLAST of Sed0024477 vs. ExPASy TrEMBL
Match: A0A5A7UUH3 (Protein-L-isoaspartate O-methyltransferase 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G00890 PE=3 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 7.4e-124
Identity = 236/306 (77.12%), Postives = 253/306 (82.68%), Query Frame = 0

Query: 1   MCCHHSVLPLRRRRRRL-SALTPTTSS------PNYLGFSPSFSSFRDLR----YLKLEI 60
           + CHH     RRRRRRL +AL PTTSS         + F P F     L       ++EI
Sbjct: 31  LSCHHH---RRRRRRRLPTALPPTTSSVPKTTVTTIISFLPRFLDPHFLTGNCLLSRMEI 90

Query: 61  LGYTKMDKMPAQPFSSLKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFA 120
           LG+ KMDK+P + F++LKRFWTGSGIGTNKDMVE LQ  GVV SKKV EVME+IDRAFF 
Sbjct: 91  LGFLKMDKLPPRSFTTLKRFWTGSGIGTNKDMVEKLQTYGVVRSKKVSEVMENIDRAFFV 150

Query: 121 PNGTSPYVDTPVPIGYNVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALM 180
           P+   PYVDTPVPIGYN TISAPHMHATCLQLLEKHLQPGM ALDVGSGTGYLTACFALM
Sbjct: 151 PDDVPPYVDTPVPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALM 210

Query: 181 VGPEGRAVGVEHIPELVASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHV 240
           VGPEGR VGVEHIPELVASS+ENI KSAA PLLKEGSLS+HVGDGRQGWAECAPYDAIHV
Sbjct: 211 VGPEGRVVGVEHIPELVASSMENIKKSAAAPLLKEGSLSLHVGDGRQGWAECAPYDAIHV 270

Query: 241 GAAAAEIPAALIEQLKPGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSRE 296
           GAAA EIP ALI+QLKPGGRMVIPVGNIFQDLKVVDKD DGSV+IHDE SVRYVPLTSRE
Sbjct: 271 GAAATEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDSDGSVSIHDETSVRYVPLTSRE 330

BLAST of Sed0024477 vs. ExPASy TrEMBL
Match: A0A6J1JIJ8 (Protein-L-isoaspartate O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484770 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 8.8e-117
Identity = 209/241 (86.72%), Postives = 222/241 (92.12%), Query Frame = 0

Query: 55  MDKMPAQPFSSLKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTS 114
           MD++P  PF +LKRFW+GSGIGTNK+MVENLQ  GVV SKKV EVMES+DRAFF P+G  
Sbjct: 1   MDELPPHPFLTLKRFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPDGVP 60

Query: 115 PYVDTPVPIGYNVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPEG 174
           PYVDTP+PIGYN TISAPHMHATCLQLLEKHLQPGM ALDVGSGTGYLTACFALMVGPEG
Sbjct: 61  PYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEG 120

Query: 175 RAVGVEHIPELVASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAA 234
           RAVGVEHIPELVASSI+NI KSAA PLLK+GSLSVHVGDGRQGWAECAPYDAIHVGAAAA
Sbjct: 121 RAVGVEHIPELVASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGAAAA 180

Query: 235 EIPAALIEQLKPGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQLRD 294
           EIP ALI+QLKPGGRMVIPVGNIFQDLKVVDKD DGS+ IHDE SVRYVPLTSREAQL D
Sbjct: 181 EIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQLGD 240

Query: 295 D 296
           +
Sbjct: 241 N 241

BLAST of Sed0024477 vs. ExPASy TrEMBL
Match: A0A6J1E441 (Protein-L-isoaspartate O-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111429755 PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 3.3e-116
Identity = 209/241 (86.72%), Postives = 221/241 (91.70%), Query Frame = 0

Query: 55  MDKMPAQPFSSLKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTS 114
           MD++P  PF +LKRFW+GSGIGTNK+MVENLQ  GVV SKKV EVMES+DRAFF P+G  
Sbjct: 1   MDELPPHPFLTLKRFWSGSGIGTNKEMVENLQNYGVVRSKKVSEVMESVDRAFFVPDGVP 60

Query: 115 PYVDTPVPIGYNVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPEG 174
           PYVDTPVPIGYN TISAPHMHATCLQLLEKHLQPGM ALDVGSGTGYLTACFALMVGP+G
Sbjct: 61  PYVDTPVPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPKG 120

Query: 175 RAVGVEHIPELVASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAA 234
           RAVGVEHIPELVASSI+NI KSAA PLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAA
Sbjct: 121 RAVGVEHIPELVASSIKNIEKSAAAPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAA 180

Query: 235 EIPAALIEQLKPGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQLRD 294
           EIP ALI+QLKPGGRMVIPVGNIFQDLKVVDKD DGS+ I DE SVRYVPLTSREAQL D
Sbjct: 181 EIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIRDETSVRYVPLTSREAQLGD 240

Query: 295 D 296
           +
Sbjct: 241 N 241

BLAST of Sed0024477 vs. ExPASy TrEMBL
Match: A0A1S3CLQ7 (Protein-L-isoaspartate O-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103502358 PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 5.7e-116
Identity = 228/302 (75.50%), Postives = 241/302 (79.80%), Query Frame = 0

Query: 1   MCCHHSVLPLRRRRRRL-SALTPTTSS------PNYLGFSPSFSSFRDLRYLKLEILGYT 60
           + CHH     RRRRRRL +AL PTTSS         + F P    F D  +L    L   
Sbjct: 31  LSCHH-----RRRRRRLPTALPPTTSSVPKTTVTTIISFLP---RFLDPHFLTGNCL--- 90

Query: 61  KMDKMPAQPFSSLKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGT 120
                     S ++RFWTGSGIGTNKDMVE LQ  GVV SKKV EVME+IDRAFF P+  
Sbjct: 91  ---------LSRMERFWTGSGIGTNKDMVEKLQTYGVVRSKKVSEVMENIDRAFFVPDDV 150

Query: 121 SPYVDTPVPIGYNVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPE 180
            PYVDTPVPIGYN TISAPHMHATCLQLLEKHLQPGM ALDVGSGTGYLTACFALMVGPE
Sbjct: 151 PPYVDTPVPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPE 210

Query: 181 GRAVGVEHIPELVASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAA 240
           GR VGVEHIPELVASS+ENI KSAA PLLKEGSLS+HVGDGRQGWAECAPYDAIHVGAAA
Sbjct: 211 GRVVGVEHIPELVASSMENIKKSAAAPLLKEGSLSLHVGDGRQGWAECAPYDAIHVGAAA 270

Query: 241 AEIPAALIEQLKPGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQLR 296
           AEIP ALI+QLKPGGRMVIPVGNIFQDLKVVDKD DGSV+IHDE SVRYVPLTSREAQLR
Sbjct: 271 AEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDSDGSVSIHDETSVRYVPLTSREAQLR 312

BLAST of Sed0024477 vs. TAIR 10
Match: AT3G48330.1 (protein-l-isoaspartate methyltransferase 1 )

HSP 1 Score: 355.1 bits (910), Expect = 5.3e-98
Identity = 174/230 (75.65%), Postives = 198/230 (86.09%), Query Frame = 0

Query: 66  LKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTSPYVDTPVPIGY 125
           +K+FW+ S I  NK MVENLQ  G+V+S +V + ME++DR  F  + +S YVD+P+ IGY
Sbjct: 1   MKQFWSPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGY 60

Query: 126 NVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPEGRAVGVEHIPEL 185
           NVTISAPHMHA CLQLLEKHL+PGM  LDVGSGTGYLTACFA+MVG EGRA+GVEHIPEL
Sbjct: 61  NVTISAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPEL 120

Query: 186 VASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPAALIEQLK 245
           VASS++NI  SAA P LKEGSL+VHVGDGRQGWAE APYDAIHVGAAA EIP ALI+QLK
Sbjct: 121 VASSVKNIEASAASPFLKEGSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLK 180

Query: 246 PGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQLRDD 296
           PGGR+VIPVGNIFQDL+VVDK+ DGSV+I DE SVRYVPLTSREAQLR D
Sbjct: 181 PGGRLVIPVGNIFQDLQVVDKNSDGSVSIKDETSVRYVPLTSREAQLRGD 230

BLAST of Sed0024477 vs. TAIR 10
Match: AT3G48330.2 (protein-l-isoaspartate methyltransferase 1 )

HSP 1 Score: 355.1 bits (910), Expect = 5.3e-98
Identity = 174/230 (75.65%), Postives = 198/230 (86.09%), Query Frame = 0

Query: 66  LKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTSPYVDTPVPIGY 125
           +K+FW+ S I  NK MVENLQ  G+V+S +V + ME++DR  F  + +S YVD+P+ IGY
Sbjct: 1   MKQFWSPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGY 60

Query: 126 NVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPEGRAVGVEHIPEL 185
           NVTISAPHMHA CLQLLEKHL+PGM  LDVGSGTGYLTACFA+MVG EGRA+GVEHIPEL
Sbjct: 61  NVTISAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPEL 120

Query: 186 VASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPAALIEQLK 245
           VASS++NI  SAA P LKEGSL+VHVGDGRQGWAE APYDAIHVGAAA EIP ALI+QLK
Sbjct: 121 VASSVKNIEASAASPFLKEGSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLK 180

Query: 246 PGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQLRDD 296
           PGGR+VIPVGNIFQDL+VVDK+ DGSV+I DE SVRYVPLTSREAQLR D
Sbjct: 181 PGGRLVIPVGNIFQDLQVVDKNSDGSVSIKDETSVRYVPLTSREAQLRGD 230

BLAST of Sed0024477 vs. TAIR 10
Match: AT5G50240.1 (protein-l-isoaspartate methyltransferase 2 )

HSP 1 Score: 330.5 bits (846), Expect = 1.4e-90
Identity = 171/291 (58.76%), Postives = 213/291 (73.20%), Query Frame = 0

Query: 10  LRRRRRRLSA---LTPTTSSPNYLGFSPS-----FSSFRDLRYLKLEILGYTKMDKMPAQ 69
           +++R++++ A   L PT ++   L  +P       SS    ++L L       + +    
Sbjct: 22  IKKRKKKMRAQVLLCPTITAYGRLYCAPRRLHRYNSSSSSSQFLNL------NLSRFSGA 81

Query: 70  PFSSLKRFWTGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTSPYVDTPV 129
            F  +++F +G+G    + MVENL++ GV+SSK+V +VME++DR  F P G+S YVDTPV
Sbjct: 82  LFFHMEQFQSGTGSSGKRGMVENLKRYGVISSKRVAQVMEALDRGLFVPVGSSAYVDTPV 141

Query: 130 PIGYNVTISAPHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPEGRAVGVEH 189
           PIGYN TISAPHMHATCLQLLE  L PGM ALDVGSGTGYLT CFALMVG EGR VGV+H
Sbjct: 142 PIGYNATISAPHMHATCLQLLEDKLHPGMRALDVGSGTGYLTGCFALMVGAEGRVVGVDH 201

Query: 190 IPELVASSIENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPAALI 249
           IPELV  SI+NI KS A   LK+GSLS+HVGDGR+GW E APYDAIHVGAAA+EIP  L+
Sbjct: 202 IPELVDMSIKNIEKSVAASFLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAAASEIPQPLL 261

Query: 250 EQLKPGGRMVIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQL 293
           +QLKPGGRMVIP+G  FQ+LKV+DK+ DGS+ +H E SVRYVPLTSR  QL
Sbjct: 262 DQLKPGGRMVIPLGTYFQELKVIDKNEDGSIKVHTETSVRYVPLTSRVEQL 306

BLAST of Sed0024477 vs. TAIR 10
Match: AT5G50240.2 (protein-l-isoaspartate methyltransferase 2 )

HSP 1 Score: 325.5 bits (833), Expect = 4.5e-89
Identity = 158/222 (71.17%), Postives = 183/222 (82.43%), Query Frame = 0

Query: 71  TGSGIGTNKDMVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTSPYVDTPVPIGYNVTIS 130
           +G+G    + MVENL++ GV+SSK+V +VME++DR  F P G+S YVDTPVPIGYN TIS
Sbjct: 82  SGTGSSGKRGMVENLKRYGVISSKRVAQVMEALDRGLFVPVGSSAYVDTPVPIGYNATIS 141

Query: 131 APHMHATCLQLLEKHLQPGMHALDVGSGTGYLTACFALMVGPEGRAVGVEHIPELVASSI 190
           APHMHATCLQLLE  L PGM ALDVGSGTGYLT CFALMVG EGR VGV+HIPELV  SI
Sbjct: 142 APHMHATCLQLLEDKLHPGMRALDVGSGTGYLTGCFALMVGAEGRVVGVDHIPELVDMSI 201

Query: 191 ENINKSAADPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPAALIEQLKPGGRM 250
           +NI KS A   LK+GSLS+HVGDGR+GW E APYDAIHVGAAA+EIP  L++QLKPGGRM
Sbjct: 202 KNIEKSVAASFLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAAASEIPQPLLDQLKPGGRM 261

Query: 251 VIPVGNIFQDLKVVDKDVDGSVTIHDEISVRYVPLTSREAQL 293
           VIP+G  FQ+LKV+DK+ DGS+ +H E SVRYVPLTSR  QL
Sbjct: 262 VIPLGTYFQELKVIDKNEDGSIKVHTETSVRYVPLTSRVEQL 303

BLAST of Sed0024477 vs. TAIR 10
Match: AT5G50240.3 (protein-l-isoaspartate methyltransferase 2 )

HSP 1 Score: 321.2 bits (822), Expect = 8.5e-88
Identity = 156/212 (73.58%), Postives = 178/212 (83.96%), Query Frame = 0

Query: 81  MVENLQKCGVVSSKKVFEVMESIDRAFFAPNGTSPYVDTPVPIGYNVTISAPHMHATCLQ 140
           MVENL++ GV+SSK+V +VME++DR  F P G+S YVDTPVPIGYN TISAPHMHATCLQ
Sbjct: 1   MVENLKRYGVISSKRVAQVMEALDRGLFVPVGSSAYVDTPVPIGYNATISAPHMHATCLQ 60

Query: 141 LLEKHLQPGMHALDVGSGTGYLTACFALMVGPEGRAVGVEHIPELVASSIENINKSAADP 200
           LLE  L PGM ALDVGSGTGYLT CFALMVG EGR VGV+HIPELV  SI+NI KS A  
Sbjct: 61  LLEDKLHPGMRALDVGSGTGYLTGCFALMVGAEGRVVGVDHIPELVDMSIKNIEKSVAAS 120

Query: 201 LLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPAALIEQLKPGGRMVIPVGNIFQD 260
            LK+GSLS+HVGDGR+GW E APYDAIHVGAAA+EIP  L++QLKPGGRMVIP+G  FQ+
Sbjct: 121 FLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAAASEIPQPLLDQLKPGGRMVIPLGTYFQE 180

Query: 261 LKVVDKDVDGSVTIHDEISVRYVPLTSREAQL 293
           LKV+DK+ DGS+ +H E SVRYVPLTSR  QL
Sbjct: 181 LKVIDKNEDGSIKVHTETSVRYVPLTSRVEQL 212

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_016903206.14.0e-12477.45PREDICTED: protein-L-isoaspartate O-methyltransferase 1 isoform X1 [Cucumis melo... [more]
KAA0057806.11.5e-12377.12protein-L-isoaspartate O-methyltransferase 1 isoform X1 [Cucumis melo var. makuw... [more]
XP_023515897.13.3e-11886.18protein-L-isoaspartate O-methyltransferase 1-like isoform X5 [Cucurbita pepo sub... [more]
XP_031736272.11.8e-11687.14protein-L-isoaspartate O-methyltransferase 1 isoform X2 [Cucumis sativus][more]
XP_022987123.11.8e-11686.72protein-L-isoaspartate O-methyltransferase 1-like isoform X5 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q425397.4e-9775.65Protein-L-isoaspartate O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q64J172.0e-8958.76Protein-L-isoaspartate O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q432091.3e-8867.69Protein-L-isoaspartate O-methyltransferase OS=Triticum aestivum OX=4565 GN=PCM P... [more]
Q5F3N19.8e-5753.54Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Gallus gallus OX=9031... [more]
P808951.7e-5653.60Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Sus scrofa OX=9823 GN... [more]
Match NameE-valueIdentityDescription
A0A1S4E4P71.9e-12477.45protein-L-isoaspartate O-methyltransferase 1 isoform X1 OS=Cucumis melo OX=3656 ... [more]
A0A5A7UUH37.4e-12477.12Protein-L-isoaspartate O-methyltransferase 1 isoform X1 OS=Cucumis melo var. mak... [more]
A0A6J1JIJ88.8e-11786.72Protein-L-isoaspartate O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1E4413.3e-11686.72Protein-L-isoaspartate O-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A1S3CLQ75.7e-11675.50Protein-L-isoaspartate O-methyltransferase OS=Cucumis melo OX=3656 GN=LOC1035023... [more]
Match NameE-valueIdentityDescription
AT3G48330.15.3e-9875.65protein-l-isoaspartate methyltransferase 1 [more]
AT3G48330.25.3e-9875.65protein-l-isoaspartate methyltransferase 1 [more]
AT5G50240.11.4e-9058.76protein-l-isoaspartate methyltransferase 2 [more]
AT5G50240.24.5e-8971.17protein-l-isoaspartate methyltransferase 2 [more]
AT5G50240.38.5e-8873.58protein-l-isoaspartate methyltransferase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000682Protein-L-isoaspartate(D-aspartate) O-methyltransferaseTIGRFAMTIGR00080TIGR00080coord: 77..291
e-value: 1.6E-64
score: 215.6
IPR000682Protein-L-isoaspartate(D-aspartate) O-methyltransferasePANTHERPTHR11579PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASEcoord: 56..294
IPR000682Protein-L-isoaspartate(D-aspartate) O-methyltransferasePROSITEPS01279PCMTcoord: 212..227
NoneNo IPR availablePFAMPF01135PCMTcoord: 78..289
e-value: 1.1E-83
score: 280.2
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 70..294
e-value: 1.3E-77
score: 262.6
NoneNo IPR availablePANTHERPTHR11579:SF26L-ISOASPARTATE METHYLTRANSFERASEcoord: 56..294
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 153..254
e-value: 1.631E-5
score: 41.2615
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 78..263

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0024477.1Sed0024477.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006479 protein methylation
biological_process GO:0006464 cellular protein modification process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004719 protein-L-isoaspartate (D-aspartate) O-methyltransferase activity