Sed0024349 (gene) Chayote v1

Overview


Name	Sed0024349
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Omega-amidase
Location	LG05: 31459408 .. 31463776 (+)
RNA-Seq Expression	Sed0024349
Synteny	Sed0024349

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAGACTGTTTTGAGGGGTAGGTTGAACAGTCTTAACAACTGGCGTCAATTTTAGCAGCCAAGTTCCGCTGCCACTCCACCAAGTCGTCGCCGATTATGGTGTCATCCTTCAATCCCGAGCACCTGCACTTCCTTTGCTGATCCCTCCCATCACCAAGGTTAGTTTTACTGGTGAACATGGTCGGTTGCATTACTTTAATTTTTAGGACTAGTCTAAAGTTTTGAGTTCGTCAATCTTCTAACTCGTCCAATCCTATGCATTAGAGCAACTCAATTCAACCCTATACTGAGTTGGGTTAATGTATAAACAAATAAAAGATGATGATTCATGCATGGTAGATTACTTATTTTATCGGTGCATGTATAAAAAAATCTATCTTATGTATTATTTTGGTCAATCCAACCACGAACCCGACCCATTCCATCTAAAATTAGAAAACTATAAACCATTTCAACCCTTGGATAGTTTGGGTGGCTCGGGTTGTCAGTTATTTTATACTTGTATACCTACTTAGTTTGTCTTGAGAAATCGAAGTTTTGCTCCACTATAATTGGAATAACCTTGCCTGTCTCTAGGCTGGACATTGAAGGAATGGAGATGGAAGGTGTCTACAAATTAATGTCACGGGTGATTCTCGGATTCTGAGGGGACCAACACATTTAAAGCCAAGCCTTCAACCATTGCACCACCGACACTTATTTTCGCTTCTTTGATTAATGTGATCGTCCGTGTTTCTGGTTTGTTTCGGTGTAGTTTAAGATCGGGCTGTGCCAATTGACGGCGACGACTGATAAGCAAAGGAATATTGAACATGCTCGTGAAGCCATTGTGGAGGCTGTGGAAAAGGGCGCTCAGCTTGTTGTCTTGCCGGTGAGCTTTTAGATTTTGAAAATGATAATTGTTTATTGCATTTTACATCTTCCTAGTGGTATTTCTGTTCTGCTTGTGATATCCTTTTCATATCTATGTGACAGGAATTATGGAATCGTCCTTATTCGAATGATAGCTTCCCAGTTTATGCTGAGGACATTGGAGCCAGTGGCGATGCATCTCCTTCAACAGCAATGTTATCTGAAGTTTCTCGTCGTTTAAAGGTCACGATCATCGGTGGCTAAATCGCAGAGCGATCGGGGGATAAATTGTACAATGCTTGTTGTGTGTTTGGTACTGATGGAAAGCTGAAAGCTAAACATAGGAAGGTATACAATCATATCAATACGTGGTTTGTGACTCTTGTGATGGAGTTTTAGCATTTGAGATGCATTAAAAAATTTCCTAATTTTATTAGGGGGATGATGATTTGAGGCCTTGATGAATCTCTTCCGTTGTTTAGTGTAAATTTTTTAATGTGTTTCCTCGTAGTTTCACTTATTGATGTTTAGGTGAGTCAACCATCATAGAGTGACCTAGTGGTCTAGAGGGCCTTGAGTAGCTAAAAGATAATGGGTTCAATCTATCTACCTAGGAATTAATTTCCTACGAGTTTCTTTGACATCCAAATATTGTAAAGTCAGGTGGTATGTCTTCTGACAATAGTCGAGGAGACCACAAGTTGGCCTGAACATTAACGGATGTCAAAAAAAAAAAAAAAGAACATGTGGACTGGAGTCTGATAGTGATATAAGATATCTTCTTATCCCCAAAACCGATTCATCTTTTTGTAACTAGACAATTTTGAAAGGATTAAATAATTTTTAAATTCCTGAACCGTACCTGTGCACGTTTCATCTATGGAATTTCAATCTTTAGCATACTCTTCAGTCTTGGCTACATGGACTTAAATTTGGAAATTAACATTTTGGTGTTTTCTTGTTTGTAATTCATCACTCGCAAGTTTATTATGCTCTTTTCAACTGCAGATACATCTTTTTAATATTGATATTCCTGGAAAGATCACTTTTATCGAGTCTAAGACCTTTACAGCAGGTCAAACACCTACTGTTGTGGACACAGGTTGGTTTGTGGTTCTTTTACTTTTCATGGAAGTTCTTGTAACTAGCACTTGATTACGATAAGCTTAACGACTATTGAGTTCCATCAAAAGAATAAGTATTGTTAATTGTGGGGGGCATTGAGAGGGAAATAATAGAATCTTTTGAGGGGTTGAGAGGTCTCCAAATGATGTCGTTTGGTCTCTTATTAGATTCTATGTTTCTCATTAGGCTTTGATGGTGATTTTATTTTATTTTTGTAATTATTCTTTAGGCTTCGGTAGGTTGCCTTTTTTGAATGCCTTTGTATTCACTTGTTCTTAATGAATGCTTGGTTTATTATACAAAAAGTATTGTTCTTTGTTGTTGGATAATTAACTATTTGCTGGTTCATGCAGAAGTTGGCCGTATTGGCATTGGAATCTGTTATGACGTTCGTTTTCCAGAACTTGCCATGATATATGCTGCAAGAGGTTTTCCCATGAACATCTTATCTTAAAAGAAAATATCAGTCACCTATGAACATCTTATCTTTTCTCATAATAGTGAAATTTGGCTTACATTAGCACTCTAGTTTATACATTAACCATATAATCATTTTCATTTTGTCAATAGTAGTGACTCTCTCTCCCCCATATATACATTCCTTGTGAGATTATTAACTCCAGACAGTAAAAGATGTATTCCTTAACTTCTGTTTATAATAGTTGCAGATGTTGATTATGAATTTACAACATTTCAGTATCTATGCTATTCATCGATCTCTTATGGTGCATACCTGCATTTTGTTAGAGCAGAGTGAAATTATGTGTATGCACATATTTTGGCTTGATTAACATGGTCTAATAAAAGATGCATCTTTGAGGGGTAAATCAGATTTTTTTTTTTTTTAAAACTCAGATAGCTCATAGATTTTTTTTGAAACAAGGGTAACCACGCCTGTCCCTAGGCTAGGCACCGGGAGACATCAAAGGAGTAATGTCACAGCTGAGTTTCGAGGGACCTTGAGGGGAGCATACCCTCAAAGCCCAAGTCTTCAACCACTGTGCCACCCCTTGGGGACCAGATAGCTCATAGATTGTTGTGGTGGCCATACAAAATCAATCCTTTTTATTGAAGTTGACCTTATGTCGACTGTCCTGATAGAACCATTTTCCTGCTGACACTAACTAGTTGTTAACTTCAGGTGCTCACTTGATTTGCTATCCTGGAGCATTTAACATGACTACTGGACCATTGCACTGGGAATTATTGCAGCGTGCAAGGTACAAACTTCTTTATTAATTTTCTGCTGTTATGATTTGTTGCTTATGTTTGTCGACATAACTTGATGTCGTGAACATGTTAATTAGTATAAAGATGGTTCGATCTTCAAGATGTGAGTTTTAGTTTATACCCCCTGCATATGATGCATAAAATACAGAGTAAGATTCTTTCTGGGATATCATATATTGACTACAACTACCAACAGTTTTGAGTACAAAGTCATCTTTTTTCAAACAAGGGTTTAGGGGTTAATCGTTGGTTTCTTTAAGAAAAAGGGAAAAAGAAAGTTTCAACATTCATTACCTTGTGACCACACCACATTTGGTTATTTGACCATTCGATAATCTGATTCTTTTGGCCTATTCTCTTTTAAGATAAAATTTTAGATAATTGAAATATTTGGTTGTTTTAACTGATATTGAGCTTTGATGTTTTGTGCAGGGCTGCAGATAATCAGGTATTCTACGAGACTCAAGCTTTCGTTCTTGTCTGTTTTATGCTCAAACAAAGCAATATTAAAGAAGAAATGATATCAATATGACATTGTTGGTTGTGTAGCTGAATTTCCATTGACAAAAATACTACATAGGTTATAAAGAAGCTATTAAAATAATAATTGTTTACTGTGAGATTTGTCTTGCAGTCTTGAAGTATTTTTTCTTAATAAATGGTTCATTATAACGTTTTTGAAAACTGAAGAACGTATGCATAGTTTCCAAAAACGTACAGAAGAATCTTTTTGTCTGGTCGGTATACAGAACAATTTTTCATTGATGATTTTTGTCGTTTAGTTGTATGTGGCAACTTGTTCACCTACTCGAGATGTTGGAGCTGATTATGTAGCTTGGGGCCACTCAACCCTTGTTGGGCCATTATGTATTTTTTTCCACAAAATGATTATAACGTATGCATGTTGCAGTTTGGGGAAGTGTTAGCCACAACAGAACATGATGAAGCAATAATCTTAGCGGAGATTGATTATTCACTTGTTGAGCTTAGAAGGTGATCCGTCTTTCAGACCTTTTAAGAAGTGTTTTTCATTCATTATTCAACTCTCTCTCTTATTGATAGTATGCTTAAACTATTCAGGACCAACCTACCATTTCCGAAGCAAAAGCGTGGTGATCTTTACCAGTTGGTAGATGTTCTGAGGCTGAGATCCGAATGA

mRNA sequence

ATGTCAGACTGTTTTGAGGGCCAAGTTCCGCTGCCACTCCACCAAGTCGTCGCCGATTATGGTGTCATCCTTCAATCCCGAGCACCTGCACTTCCTTTGCTGATCCCTCCCATCACCAAGTTTAAGATCGGGCTGTGCCAATTGACGGCGACGACTGATAAGCAAAGGAATATTGAACATGCTCGTGAAGCCATTGTGGAGGCTGTGGAAAAGGGCGCTCAGCTTGTTGTCTTGCCGGAATTATGGAATCGTCCTTATTCGAATGATAGCTTCCCAGTTTATGCTGAGGACATTGGAGCCAGTGGCGATGCATCTCCTTCAACAGCAATGTTATCTGAAGTTTCTCGTCGTTTAAAGCGATCGGGGGATAAATTGTACAATGCTTGTTGTGTGTTTGGTACTGATGGAAAGCTGAAAGCTAAACATAGGAAGATACATCTTTTTAATATTGATATTCCTGGAAAGATCACTTTTATCGAGTCTAAGACCTTTACAGCAGGTCAAACACCTACTGTTGTGGACACAGAAGTTGGCCGTATTGGCATTGGAATCTGTTATGACGTTCGTTTTCCAGAACTTGCCATGATATATGCTGCAAGAGGTGCTCACTTGATTTGCTATCCTGGAGCATTTAACATGACTACTGGACCATTGCACTGGGAATTATTGCAGCGTGCAAGGGCTGCAGATAATCAGTTGTATGTGGCAACTTGTTCACCTACTCGAGATGTTGGAGCTGATTATGTAGCTTGGGGCCACTCAACCCTTTTTGGGGAAGTGTTAGCCACAACAGAACATGATGAAGCAATAATCTTAGCGGAGATTGATTATTCACTTGTTGAGCTTAGAAGGACCAACCTACCATTTCCGAAGCAAAAGCGTGGTGATCTTTACCAGTTGGTAGATGTTCTGAGGCTGAGATCCGAATGA

Coding sequence (CDS)

ATGTCAGACTGTTTTGAGGGCCAAGTTCCGCTGCCACTCCACCAAGTCGTCGCCGATTATGGTGTCATCCTTCAATCCCGAGCACCTGCACTTCCTTTGCTGATCCCTCCCATCACCAAGTTTAAGATCGGGCTGTGCCAATTGACGGCGACGACTGATAAGCAAAGGAATATTGAACATGCTCGTGAAGCCATTGTGGAGGCTGTGGAAAAGGGCGCTCAGCTTGTTGTCTTGCCGGAATTATGGAATCGTCCTTATTCGAATGATAGCTTCCCAGTTTATGCTGAGGACATTGGAGCCAGTGGCGATGCATCTCCTTCAACAGCAATGTTATCTGAAGTTTCTCGTCGTTTAAAGCGATCGGGGGATAAATTGTACAATGCTTGTTGTGTGTTTGGTACTGATGGAAAGCTGAAAGCTAAACATAGGAAGATACATCTTTTTAATATTGATATTCCTGGAAAGATCACTTTTATCGAGTCTAAGACCTTTACAGCAGGTCAAACACCTACTGTTGTGGACACAGAAGTTGGCCGTATTGGCATTGGAATCTGTTATGACGTTCGTTTTCCAGAACTTGCCATGATATATGCTGCAAGAGGTGCTCACTTGATTTGCTATCCTGGAGCATTTAACATGACTACTGGACCATTGCACTGGGAATTATTGCAGCGTGCAAGGGCTGCAGATAATCAGTTGTATGTGGCAACTTGTTCACCTACTCGAGATGTTGGAGCTGATTATGTAGCTTGGGGCCACTCAACCCTTTTTGGGGAAGTGTTAGCCACAACAGAACATGATGAAGCAATAATCTTAGCGGAGATTGATTATTCACTTGTTGAGCTTAGAAGGACCAACCTACCATTTCCGAAGCAAAAGCGTGGTGATCTTTACCAGTTGGTAGATGTTCTGAGGCTGAGATCCGAATGA

Protein sequence

MSDCFEGQVPLPLHQVVADYGVILQSRAPALPLLIPPITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSNDSFPVYAEDIGASGDASPSTAMLSEVSRRLKRSGDKLYNACCVFGTDGKLKAKHRKIHLFNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYAARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTLFGEVLATTEHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLVDVLRLRSE

Homology

[Close]

BLAST of Sed0024349 vs. NCBI nr
Match: KAG6608344.1 (Omega-amidase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037697.1 Omega-amidase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 517.7 bits (1332), Expect = 6.9e-143
Identity = 257/294 (87.41%), Postives = 267/294 (90.82%), Query Frame = 0


Query: 29  PALPLLIPPITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSN 88
           PA+PL IPP+TKFKIGLCQLT T DKQRNIEHAR+AI EAVEKGAQLVVLPE+WN PYSN
Sbjct: 82  PAVPLPIPPVTKFKIGLCQLTVTPDKQRNIEHARKAIEEAVEKGAQLVVLPEIWNGPYSN 141

Query: 89  DSFPVYAEDIGASGDASPSTAMLSEVSRRLK----------RSGDKLYNACCVFGTDGKL 148
           DSFPVYAEDI A GDASPSTAMLSEVSRRLK          RSGDKLYNACC+FGTDGKL
Sbjct: 142 DSFPVYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSIAERSGDKLYNACCIFGTDGKL 201

Query: 149 KAKHRKIHLFNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYA 208
           KAKHRKIHLF+IDIPGKITFIESKT TAGQTPTVVDTEVGRIGIGICYD+RF ELAMIYA
Sbjct: 202 KAKHRKIHLFDIDIPGKITFIESKTLTAGQTPTVVDTEVGRIGIGICYDIRFQELAMIYA 261

Query: 209 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL-- 268
           ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSP RDVGA YVAWGHSTL  
Sbjct: 262 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPARDVGAGYVAWGHSTLVG 321

Query: 269 -FGEVLATTEHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLVDVLRLRSE 310
            FGEVLATTEHDEAII+AEIDYSL+ELRRTNLP  KQKRGDLYQLVDV RL+SE
Sbjct: 322 PFGEVLATTEHDEAIIIAEIDYSLIELRRTNLPLLKQKRGDLYQLVDVERLKSE 375

[Close]

BLAST of Sed0024349 vs. NCBI nr
Match: XP_022940145.1 (omega-amidase, chloroplastic [Cucurbita moschata] >XP_022940146.1 omega-amidase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 517.7 bits (1332), Expect = 6.9e-143
Identity = 257/294 (87.41%), Postives = 267/294 (90.82%), Query Frame = 0


Query: 29  PALPLLIPPITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSN 88
           PA+PL IPP+TKFKIGLCQLT T DKQRNIEHAR+AI EAVEKGAQLVVLPE+WN PYSN
Sbjct: 82  PAVPLPIPPVTKFKIGLCQLTVTPDKQRNIEHARKAIEEAVEKGAQLVVLPEIWNGPYSN 141

Query: 89  DSFPVYAEDIGASGDASPSTAMLSEVSRRLK----------RSGDKLYNACCVFGTDGKL 148
           DSFPVYAEDI A GDASPSTAMLSEVSRRLK          RSGDKLYNACC+FGTDGKL
Sbjct: 142 DSFPVYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSIAERSGDKLYNACCIFGTDGKL 201

Query: 149 KAKHRKIHLFNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYA 208
           KAKHRKIHLF+IDIPGKITFIESKT TAGQTPTVVDTEVGRIGIGICYD+RF ELAMIYA
Sbjct: 202 KAKHRKIHLFDIDIPGKITFIESKTLTAGQTPTVVDTEVGRIGIGICYDIRFQELAMIYA 261

Query: 209 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL-- 268
           ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSP RDVGA YVAWGHSTL  
Sbjct: 262 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPARDVGAGYVAWGHSTLVG 321

Query: 269 -FGEVLATTEHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLVDVLRLRSE 310
            FGEVLATTEHDEAII+AEIDYSL+ELRRTNLP  KQKRGDLYQLVDV RL+SE
Sbjct: 322 PFGEVLATTEHDEAIIIAEIDYSLIELRRTNLPLLKQKRGDLYQLVDVERLKSE 375

[Close]

BLAST of Sed0024349 vs. NCBI nr
Match: XP_022134239.1 (omega-amidase, chloroplastic [Momordica charantia])

HSP 1 Score: 515.4 bits (1326), Expect = 3.4e-142
Identity = 254/294 (86.39%), Postives = 268/294 (91.16%), Query Frame = 0


Query: 29  PALPLLIPPITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSN 88
           PALPL IPP++KFKIGLCQLT T+DKQRNIEHAR+AI EAVEKGAQLVVLPE+WN PYSN
Sbjct: 76  PALPLPIPPVSKFKIGLCQLTVTSDKQRNIEHARKAIEEAVEKGAQLVVLPEIWNGPYSN 135

Query: 89  DSFPVYAEDIGASGDASPSTAMLSEVSRRLK----------RSGDKLYNACCVFGTDGKL 148
           DSFPVYAEDI A GDASPSTAMLSEVSRRLK          RSGD+LYNACCVFGTDGKL
Sbjct: 136 DSFPVYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSIAERSGDRLYNACCVFGTDGKL 195

Query: 149 KAKHRKIHLFNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYA 208
           KAKHRKIHLF+IDIPGKITFIESKT TAGQTPT+VDTEVG IGIGICYD+RFPELAMIYA
Sbjct: 196 KAKHRKIHLFDIDIPGKITFIESKTLTAGQTPTIVDTEVGXIGIGICYDIRFPELAMIYA 255

Query: 209 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL-- 268
           ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSP RDVGA YVAWGHSTL  
Sbjct: 256 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPARDVGAGYVAWGHSTLVG 315

Query: 269 -FGEVLATTEHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLVDVLRLRSE 310
            FGE+LATTEH+EAII+AEIDYSL+ELRRTNLP  KQKRGDLYQLVDV RL+SE
Sbjct: 316 PFGEMLATTEHEEAIIIAEIDYSLIELRRTNLPLLKQKRGDLYQLVDVQRLKSE 369

[Close]

BLAST of Sed0024349 vs. NCBI nr
Match: XP_023524248.1 (omega-amidase, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023524249.1 omega-amidase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 515.0 bits (1325), Expect = 4.5e-142
Identity = 256/294 (87.07%), Postives = 266/294 (90.48%), Query Frame = 0


Query: 29  PALPLLIPPITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSN 88
           PA+PL IPP+TKFKIGLCQLT T DKQRNIEHAR+AI EAV KGAQLVVLPE+WN PYSN
Sbjct: 82  PAVPLPIPPVTKFKIGLCQLTVTPDKQRNIEHARKAIEEAVGKGAQLVVLPEIWNGPYSN 141

Query: 89  DSFPVYAEDIGASGDASPSTAMLSEVSRRLK----------RSGDKLYNACCVFGTDGKL 148
           DSFPVYAEDI A GDASPSTAMLSEVSRRLK          RSGDKLYNACC+FGTDGKL
Sbjct: 142 DSFPVYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSIAERSGDKLYNACCIFGTDGKL 201

Query: 149 KAKHRKIHLFNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYA 208
           KAKHRKIHLF+IDIPGKITFIESKT TAGQTPTVVDTEVGRIGIGICYD+RF ELAMIYA
Sbjct: 202 KAKHRKIHLFDIDIPGKITFIESKTLTAGQTPTVVDTEVGRIGIGICYDIRFQELAMIYA 261

Query: 209 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL-- 268
           ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSP RDVGA YVAWGHSTL  
Sbjct: 262 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPARDVGAGYVAWGHSTLVG 321

Query: 269 -FGEVLATTEHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLVDVLRLRSE 310
            FGEVLATTEHDEAII+AEIDYSL+ELRRTNLP  KQKRGDLYQLVDV RL+SE
Sbjct: 322 PFGEVLATTEHDEAIIIAEIDYSLIELRRTNLPLLKQKRGDLYQLVDVERLKSE 375

[Close]

BLAST of Sed0024349 vs. NCBI nr
Match: XP_022982376.1 (omega-amidase, chloroplastic [Cucurbita maxima] >XP_022982377.1 omega-amidase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 512.7 bits (1319), Expect = 2.2e-141
Identity = 255/294 (86.73%), Postives = 265/294 (90.14%), Query Frame = 0


Query: 29  PALPLLIPPITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSN 88
           PALPL IPP+TKFKIGLCQL  T DKQRNIEHAR+AI EAVEKGAQLVVLPE+WN PYSN
Sbjct: 82  PALPLPIPPVTKFKIGLCQLMVTPDKQRNIEHARKAIEEAVEKGAQLVVLPEIWNGPYSN 141

Query: 89  DSFPVYAEDIGASGDASPSTAMLSEVSRRLK----------RSGDKLYNACCVFGTDGKL 148
           DSFPVYAEDI A GDASPSTAMLSEVSRRLK          RSGDKLYNACC+FGTDGKL
Sbjct: 142 DSFPVYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSIAERSGDKLYNACCIFGTDGKL 201

Query: 149 KAKHRKIHLFNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYA 208
           KAKHRKIHLF+IDIPGKITFIESKT TAGQTPTVVDTEVGRIGIGICYD+RF ELAMIYA
Sbjct: 202 KAKHRKIHLFDIDIPGKITFIESKTLTAGQTPTVVDTEVGRIGIGICYDIRFQELAMIYA 261

Query: 209 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL-- 268
           ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLY+ATCSP RD GA YVAWGHSTL  
Sbjct: 262 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYLATCSPARDDGAGYVAWGHSTLVG 321

Query: 269 -FGEVLATTEHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLVDVLRLRSE 310
            FGEVLATTEHDEAII+AEIDYSL+ELRRTNLP  KQKRGDLYQLVDV RL+SE
Sbjct: 322 PFGEVLATTEHDEAIIIAEIDYSLIELRRTNLPLLKQKRGDLYQLVDVERLKSE 375

[Close]

BLAST of Sed0024349 vs. ExPASy Swiss-Prot
Match: Q8RUF8 (Omega-amidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NLP3 PE=1 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 5.7e-132
Identity = 229/299 (76.59%), Postives = 259/299 (86.62%), Query Frame = 0


Query: 25  QSRAP-ALPLLIPPITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWN 84
           Q+R P ALPL  PP+TKF IGLCQL+ T+DK+RNI HA++AI EA  KGA+LV+LPE+WN
Sbjct: 71  QARVPSALPLPAPPLTKFNIGLCQLSVTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWN 130

Query: 85  RPYSNDSFPVYAEDIGASGDASPSTAMLSEVSRRLK----------RSGDKLYNACCVFG 144
            PYSNDSFPVYAE+I A GDASPSTAMLSEVS+RLK          R GD+LYN CCVFG
Sbjct: 131 SPYSNDSFPVYAEEIDAGGDASPSTAMLSEVSKRLKITIIGGSIPERVGDRLYNTCCVFG 190

Query: 145 TDGKLKAKHRKIHLFNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPEL 204
           +DG+LKAKHRKIHLF+IDIPGKITF+ESKT TAG+TPT+VDT+VGRIGIGICYD+RF EL
Sbjct: 191 SDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQEL 250

Query: 205 AMIYAARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGH 264
           AMIYAARGAHL+CYPGAFNMTTGPLHWELLQRARA DNQLYVATCSP RD GA Y AWGH
Sbjct: 251 AMIYAARGAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGH 310

Query: 265 STL---FGEVLATTEHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLVDVLRLRSE 310
           STL   FGEVLATTEH+EAII+AEIDYS++E RRT+LP  +Q+RGDLYQLVDV RL S+
Sbjct: 311 STLVGPFGEVLATTEHEEAIIIAEIDYSILEQRRTSLPLNRQRRGDLYQLVDVQRLDSK 369

[Close]

BLAST of Sed0024349 vs. ExPASy Swiss-Prot
Match: Q6IR61 (Omega-amidase NIT2-A OS=Xenopus laevis OX=8355 GN=nit2a PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.0e-72
Identity = 139/276 (50.36%), Postives = 176/276 (63.77%), Query Frame = 0


Query: 38  ITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSNDSFPVYAED 97
           + KFK+ L Q   +  K  N+  A + I EA +KGAQ+V LPE +N PY    FP YAE 
Sbjct: 1   MAKFKLSLVQFLVSPVKSDNLNRACKLIKEAAQKGAQIVALPECFNSPYGTTYFPEYAEK 60

Query: 98  IGASGDASPSTAMLSEVSRRL----------KRSGDKLYNACCVFGTDGKLKAKHRKIHL 157
           I        ST +LS+V++            +    KLYN C VFG DG L  KHRKIHL
Sbjct: 61  I-----PGESTELLSQVAKECGIYLIGGSIPEEDCGKLYNTCAVFGPDGTLLVKHRKIHL 120

Query: 158 FNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYAARGAHLICY 217
           F+ID+PGKI F ES+T + G + +V DT   ++G+GICYD+RF ELA IYA +G  L+ Y
Sbjct: 121 FDIDVPGKIRFQESETLSPGDSFSVFDTPYCKVGVGICYDIRFAELAQIYANKGCQLLVY 180

Query: 218 PGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL---FGEVLATT 277
           PGAFNMTTGP HWELLQRARA DNQ+YVAT SP RD  A YVAWGHST+   +GEV+A  
Sbjct: 181 PGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVVAKA 240

Query: 278 EHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQL 301
             +E ++ AEID   +   R  +P  +Q+R DLY +
Sbjct: 241 GSEETVLSAEIDLQYLAEIREQIPIRRQRRRDLYNV 271

[Close]

BLAST of Sed0024349 vs. ExPASy Swiss-Prot
Match: Q10166 (Hydrolase C26A3.11 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC26A3.11 PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 6.6e-72
Identity = 135/274 (49.27%), Postives = 183/274 (66.79%), Query Frame = 0


Query: 41  FKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSNDSFPVYAEDIGA 100
           F+IGL QL  T DK  N++ AR  ++EA + G+ ++VLPE++N PY    F  YAE I  
Sbjct: 44  FRIGLVQLANTKDKSENLQLARLKVLEAAKNGSNVIVLPEIFNSPYGTGYFNQYAEPI-- 103

Query: 101 SGDASPSTAMLSEVSRRLK----------RSGDKLYNACCVFGTDGKLKAKHRKIHLFNI 160
             ++SPS   LS +++  K          R   KLYN   VF   GKL A HRKIHLF+I
Sbjct: 104 -EESSPSYQALSSMAKDTKTYLFGGSIPERKDGKLYNTAMVFDPSGKLIAVHRKIHLFDI 163

Query: 161 DIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYAARGAHLICYPGA 220
           DIPG ++F ES + + G   T+VDTE G+ G+GICYD+RFPELAMI A  G  ++ YPGA
Sbjct: 164 DIPGGVSFRESDSLSPGDAMTMVDTEYGKFGLGICYDIRFPELAMIAARNGCSVMIYPGA 223

Query: 221 FNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL---FGEVLATTEHD 280
           FN++TGPLHWELL RARA DN+++VA C+P RD+ ADY +WGHST+   FG+V+ATT+  
Sbjct: 224 FNLSTGPLHWELLARARAVDNEMFVACCAPARDMNADYHSWGHSTVVDPFGKVIATTDEK 283

Query: 281 EAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLV 302
            +I+ A+ID S++   R ++P   Q+R D+Y  V
Sbjct: 284 PSIVYADIDPSVMSTARNSVPIYTQRRFDVYSEV 314

[Close]

BLAST of Sed0024349 vs. ExPASy Swiss-Prot
Match: Q497B0 (Omega-amidase NIT2 OS=Rattus norvegicus OX=10116 GN=Nit2 PE=1 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.1e-71
Identity = 140/276 (50.72%), Postives = 175/276 (63.41%), Query Frame = 0


Query: 38  ITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSNDSFPVYAED 97
           ++ F++ L QL  ++ K  NI  A   + EA ++GA +V LPE +N PY  + FP YAE 
Sbjct: 1   MSTFRLALIQLQVSSIKSDNITRACSLVREAAKQGANIVSLPECFNSPYGTNYFPEYAEK 60

Query: 98  IGASGDASPSTAMLSEVSRR----------LKRSGDKLYNACCVFGTDGKLKAKHRKIHL 157
           I        ST  LSEV++            +    KLYN C VFG DG L  KHRKIHL
Sbjct: 61  I-----PGESTKKLSEVAKENSIYLIGGSIPEEDDGKLYNTCAVFGPDGNLLVKHRKIHL 120

Query: 158 FNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYAARGAHLICY 217
           F+ID+PGKITF ESKT + G + +  DT   R+G+GICYD+RF ELA IYA RG  L+ Y
Sbjct: 121 FDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYARRGCQLLVY 180

Query: 218 PGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL---FGEVLATT 277
           PGAFNMTTGP HWELLQRARA DNQ+YVAT SP RD  A YVAWGHST+   +G+VL   
Sbjct: 181 PGAFNMTTGPAHWELLQRARAVDNQVYVATASPARDEKASYVAWGHSTVVDPWGQVLTKA 240

Query: 278 EHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQL 301
             +E I+ ++ID   +   R  +P  KQKR DLY +
Sbjct: 241 GTEETILYSDIDLKKLSEIRQQIPILKQKRADLYSV 271

[Close]

BLAST of Sed0024349 vs. ExPASy Swiss-Prot
Match: Q2T9R6 (Omega-amidase NIT2 OS=Bos taurus OX=9913 GN=NIT2 PE=2 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.5e-71
Identity = 141/273 (51.65%), Postives = 177/273 (64.84%), Query Frame = 0


Query: 41  FKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSNDSFPVYAEDIGA 100
           F++ L QL  ++ K  N+  A   I EA ++GAQ+V LPE +N PY    FP YAE I  
Sbjct: 4   FRLALIQLQVSSIKSENLTRACGLIREASKQGAQIVSLPECFNSPYGTKYFPDYAEKI-- 63

Query: 101 SGDASPSTAMLSEVSRRL----------KRSGDKLYNACCVFGTDGKLKAKHRKIHLFNI 160
            GD   ST  LSEV++            ++   KLYN C VFG DG L  KHRK+HLF+I
Sbjct: 64  PGD---STQKLSEVAKECSMYVIGGSIPEKDAGKLYNTCAVFGPDGTLLVKHRKLHLFDI 123

Query: 161 DIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYAARGAHLICYPGA 220
           D+PGKITF ES+T + G + ++ DT   R+G+GICYD+RF ELA IYA RG  L+ YPGA
Sbjct: 124 DVPGKITFQESETLSPGDSFSLFDTPYCRVGLGICYDIRFAELAQIYAQRGCQLLVYPGA 183

Query: 221 FNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL---FGEVLATTEHD 280
           FN+TTGP HWELLQR RA DNQ+YVAT SP RD  A YVAWGHST+   +GEVLA    +
Sbjct: 184 FNLTTGPAHWELLQRGRAVDNQVYVATASPARDEKASYVAWGHSTVVNPWGEVLAKAGTE 243

Query: 281 EAIILAEIDYSLVELRRTNLPFPKQKRGDLYQL 301
           E I+ A+ID   +   R  +P   QKR DLY++
Sbjct: 244 ETIVYADIDLKKLAEIRQQIPIFSQKRSDLYEV 271

[Close]

BLAST of Sed0024349 vs. ExPASy TrEMBL
Match: A0A6J1FPQ5 (omega-amidase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445858 PE=4 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 3.4e-143
Identity = 257/294 (87.41%), Postives = 267/294 (90.82%), Query Frame = 0


Query: 29  PALPLLIPPITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSN 88
           PA+PL IPP+TKFKIGLCQLT T DKQRNIEHAR+AI EAVEKGAQLVVLPE+WN PYSN
Sbjct: 82  PAVPLPIPPVTKFKIGLCQLTVTPDKQRNIEHARKAIEEAVEKGAQLVVLPEIWNGPYSN 141

Query: 89  DSFPVYAEDIGASGDASPSTAMLSEVSRRLK----------RSGDKLYNACCVFGTDGKL 148
           DSFPVYAEDI A GDASPSTAMLSEVSRRLK          RSGDKLYNACC+FGTDGKL
Sbjct: 142 DSFPVYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSIAERSGDKLYNACCIFGTDGKL 201

Query: 149 KAKHRKIHLFNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYA 208
           KAKHRKIHLF+IDIPGKITFIESKT TAGQTPTVVDTEVGRIGIGICYD+RF ELAMIYA
Sbjct: 202 KAKHRKIHLFDIDIPGKITFIESKTLTAGQTPTVVDTEVGRIGIGICYDIRFQELAMIYA 261

Query: 209 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL-- 268
           ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSP RDVGA YVAWGHSTL  
Sbjct: 262 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPARDVGAGYVAWGHSTLVG 321

Query: 269 -FGEVLATTEHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLVDVLRLRSE 310
            FGEVLATTEHDEAII+AEIDYSL+ELRRTNLP  KQKRGDLYQLVDV RL+SE
Sbjct: 322 PFGEVLATTEHDEAIIIAEIDYSLIELRRTNLPLLKQKRGDLYQLVDVERLKSE 375

[Close]

BLAST of Sed0024349 vs. ExPASy TrEMBL
Match: A0A6J1BY88 (omega-amidase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006541 PE=3 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.7e-142
Identity = 254/294 (86.39%), Postives = 268/294 (91.16%), Query Frame = 0


Query: 29  PALPLLIPPITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSN 88
           PALPL IPP++KFKIGLCQLT T+DKQRNIEHAR+AI EAVEKGAQLVVLPE+WN PYSN
Sbjct: 76  PALPLPIPPVSKFKIGLCQLTVTSDKQRNIEHARKAIEEAVEKGAQLVVLPEIWNGPYSN 135

Query: 89  DSFPVYAEDIGASGDASPSTAMLSEVSRRLK----------RSGDKLYNACCVFGTDGKL 148
           DSFPVYAEDI A GDASPSTAMLSEVSRRLK          RSGD+LYNACCVFGTDGKL
Sbjct: 136 DSFPVYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSIAERSGDRLYNACCVFGTDGKL 195

Query: 149 KAKHRKIHLFNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYA 208
           KAKHRKIHLF+IDIPGKITFIESKT TAGQTPT+VDTEVG IGIGICYD+RFPELAMIYA
Sbjct: 196 KAKHRKIHLFDIDIPGKITFIESKTLTAGQTPTIVDTEVGXIGIGICYDIRFPELAMIYA 255

Query: 209 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL-- 268
           ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSP RDVGA YVAWGHSTL  
Sbjct: 256 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPARDVGAGYVAWGHSTLVG 315

Query: 269 -FGEVLATTEHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLVDVLRLRSE 310
            FGE+LATTEH+EAII+AEIDYSL+ELRRTNLP  KQKRGDLYQLVDV RL+SE
Sbjct: 316 PFGEMLATTEHEEAIIIAEIDYSLIELRRTNLPLLKQKRGDLYQLVDVQRLKSE 369

[Close]

BLAST of Sed0024349 vs. ExPASy TrEMBL
Match: A0A6J1IZ64 (omega-amidase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111481222 PE=4 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.1e-141
Identity = 255/294 (86.73%), Postives = 265/294 (90.14%), Query Frame = 0


Query: 29  PALPLLIPPITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSN 88
           PALPL IPP+TKFKIGLCQL  T DKQRNIEHAR+AI EAVEKGAQLVVLPE+WN PYSN
Sbjct: 82  PALPLPIPPVTKFKIGLCQLMVTPDKQRNIEHARKAIEEAVEKGAQLVVLPEIWNGPYSN 141

Query: 89  DSFPVYAEDIGASGDASPSTAMLSEVSRRLK----------RSGDKLYNACCVFGTDGKL 148
           DSFPVYAEDI A GDASPSTAMLSEVSRRLK          RSGDKLYNACC+FGTDGKL
Sbjct: 142 DSFPVYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSIAERSGDKLYNACCIFGTDGKL 201

Query: 149 KAKHRKIHLFNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYA 208
           KAKHRKIHLF+IDIPGKITFIESKT TAGQTPTVVDTEVGRIGIGICYD+RF ELAMIYA
Sbjct: 202 KAKHRKIHLFDIDIPGKITFIESKTLTAGQTPTVVDTEVGRIGIGICYDIRFQELAMIYA 261

Query: 209 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL-- 268
           ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLY+ATCSP RD GA YVAWGHSTL  
Sbjct: 262 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYLATCSPARDDGAGYVAWGHSTLVG 321

Query: 269 -FGEVLATTEHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLVDVLRLRSE 310
            FGEVLATTEHDEAII+AEIDYSL+ELRRTNLP  KQKRGDLYQLVDV RL+SE
Sbjct: 322 PFGEVLATTEHDEAIIIAEIDYSLIELRRTNLPLLKQKRGDLYQLVDVERLKSE 375

[Close]

BLAST of Sed0024349 vs. ExPASy TrEMBL
Match: A0A1S3BUK3 (omega-amidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493689 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.3e-134
Identity = 240/294 (81.63%), Postives = 258/294 (87.76%), Query Frame = 0


Query: 29  PALPLLIPPITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSN 88
           PALPL IPP+TKFKI LCQL  T DKQRNIEHAR+AI EAVEKGAQLVVLPE+WN PYS+
Sbjct: 84  PALPLSIPPLTKFKIALCQLAVTADKQRNIEHARKAIEEAVEKGAQLVVLPEIWNCPYSH 143

Query: 89  DSFPVYAEDIGASGDASPSTAMLSEVSRRLK----------RSGDKLYNACCVFGTDGKL 148
           D  P+YAEDI A GDASPSTAMLSEVSRRLK          RSGDKLYN+ CVFGTDGKL
Sbjct: 144 DCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSIVERSGDKLYNSSCVFGTDGKL 203

Query: 149 KAKHRKIHLFNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYA 208
           KAKHRKIHLF+IDIPGKITFIESKT  AG+TPTVVDTEVGRIGIGICYD+RFPELAM+YA
Sbjct: 204 KAKHRKIHLFDIDIPGKITFIESKTIAAGETPTVVDTEVGRIGIGICYDIRFPELAMMYA 263

Query: 209 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL-- 268
           ARGAHLICYPGAFNMTTGPLHWELLQR+RA DNQLYVATCSP R+  A YVAWGHSTL  
Sbjct: 264 ARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYVATCSPARNADASYVAWGHSTLVG 323

Query: 269 -FGEVLATTEHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLVDVLRLRSE 310
            FGEVLATTEHDEAI+++EIDYS +ELRRTNLP  KQ+RGDLYQLVDV RL+SE
Sbjct: 324 PFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQRRGDLYQLVDVQRLKSE 377

[Close]

BLAST of Sed0024349 vs. ExPASy TrEMBL
Match: A0A0A0L5D9 (CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G646400 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 6.3e-134
Identity = 239/294 (81.29%), Postives = 256/294 (87.07%), Query Frame = 0


Query: 29  PALPLLIPPITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSN 88
           PALPL IPP+TKFKI LCQL  T DKQRNIEHAR+AI EAVEKGAQLVVLPE+WN PYS 
Sbjct: 81  PALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAIEEAVEKGAQLVVLPEIWNCPYSP 140

Query: 89  DSFPVYAEDIGASGDASPSTAMLSEVSRRLK----------RSGDKLYNACCVFGTDGKL 148
           D  P+YAEDI A GDASPSTAMLSEVSRRLK          RSGDK+YN+ CVFGTDGKL
Sbjct: 141 DCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSIVERSGDKIYNSSCVFGTDGKL 200

Query: 149 KAKHRKIHLFNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPELAMIYA 208
           KAKHRKIHLF+IDIPGKITFIESKT  AGQTPTVVDTEVGRIGIGICYD+RFPELAM+YA
Sbjct: 201 KAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDTEVGRIGIGICYDIRFPELAMMYA 260

Query: 209 ARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL-- 268
           ARGAHLICYPGAFNMTTGPLHWELLQR+RA DNQLYVATCSP R+  A YVAWGHSTL  
Sbjct: 261 ARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYVATCSPARNADASYVAWGHSTLVG 320

Query: 269 -FGEVLATTEHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLVDVLRLRSE 310
            FGEVLATTEHDEAI+++EIDYS +ELRRTNLP  KQ+RGDLYQLVD  RL+SE
Sbjct: 321 PFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQRRGDLYQLVDAQRLKSE 374

[Close]

BLAST of Sed0024349 vs. TAIR 10
Match: AT5G12040.1 (Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein )

HSP 1 Score: 471.9 bits (1213), Expect = 4.1e-133
Identity = 229/299 (76.59%), Postives = 259/299 (86.62%), Query Frame = 0


Query: 25  QSRAP-ALPLLIPPITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWN 84
           Q+R P ALPL  PP+TKF IGLCQL+ T+DK+RNI HA++AI EA  KGA+LV+LPE+WN
Sbjct: 71  QARVPSALPLPAPPLTKFNIGLCQLSVTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWN 130

Query: 85  RPYSNDSFPVYAEDIGASGDASPSTAMLSEVSRRLK----------RSGDKLYNACCVFG 144
            PYSNDSFPVYAE+I A GDASPSTAMLSEVS+RLK          R GD+LYN CCVFG
Sbjct: 131 SPYSNDSFPVYAEEIDAGGDASPSTAMLSEVSKRLKITIIGGSIPERVGDRLYNTCCVFG 190

Query: 145 TDGKLKAKHRKIHLFNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPEL 204
           +DG+LKAKHRKIHLF+IDIPGKITF+ESKT TAG+TPT+VDT+VGRIGIGICYD+RF EL
Sbjct: 191 SDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQEL 250

Query: 205 AMIYAARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGH 264
           AMIYAARGAHL+CYPGAFNMTTGPLHWELLQRARA DNQLYVATCSP RD GA Y AWGH
Sbjct: 251 AMIYAARGAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGH 310

Query: 265 STL---FGEVLATTEHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLVDVLRLRSE 310
           STL   FGEVLATTEH+EAII+AEIDYS++E RRT+LP  +Q+RGDLYQLVDV RL S+
Sbjct: 311 STLVGPFGEVLATTEHEEAIIIAEIDYSILEQRRTSLPLNRQRRGDLYQLVDVQRLDSK 369

[Close]

BLAST of Sed0024349 vs. TAIR 10
Match: AT5G12040.2 (Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein )

HSP 1 Score: 354.8 bits (909), Expect = 7.2e-98
Identity = 169/221 (76.47%), Postives = 193/221 (87.33%), Query Frame = 0


Query: 25  QSRAP-ALPLLIPPITKFKIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWN 84
           Q+R P ALPL  PP+TKF IGLCQL+ T+DK+RNI HA++AI EA  KGA+LV+LPE+WN
Sbjct: 71  QARVPSALPLPAPPLTKFNIGLCQLSVTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWN 130

Query: 85  RPYSNDSFPVYAEDIGASGDASPSTAMLSEVSRRLK----------RSGDKLYNACCVFG 144
            PYSNDSFPVYAE+I A GDASPSTAMLSEVS+RLK          R GD+LYN CCVFG
Sbjct: 131 SPYSNDSFPVYAEEIDAGGDASPSTAMLSEVSKRLKITIIGGSIPERVGDRLYNTCCVFG 190

Query: 145 TDGKLKAKHRKIHLFNIDIPGKITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPEL 204
           +DG+LKAKHRKIHLF+IDIPGKITF+ESKT TAG+TPT+VDT+VGRIGIGICYD+RF EL
Sbjct: 191 SDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQEL 250

Query: 205 AMIYAARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLY 235
           AMIYAARGAHL+CYPGAFNMTTGPLHWELLQRARA DNQ++
Sbjct: 251 AMIYAARGAHLLCYPGAFNMTTGPLHWELLQRARATDNQVH 291

[Close]

BLAST of Sed0024349 vs. TAIR 10
Match: AT4G08790.1 (Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein )

HSP 1 Score: 117.9 bits (294), Expect = 1.5e-26
Identity = 75/265 (28.30%), Postives = 126/265 (47.55%), Query Frame = 0


Query: 42  KIGLCQLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPYSNDSFPV-YAEDIGA 101
           ++   Q+T+  D   N       + EA   GA+L+  PE ++     +   V  AE +  
Sbjct: 38  RVAAAQMTSVNDLMTNFATCSRLVQEAALAGAKLICFPENFSFVGDKEGESVKIAEPLDG 97

Query: 102 ------SGDASPSTAMLSEVSRRLKRSGDKLYNACCVFGTDGKLKAKHRKIHLFNIDIPG 161
                    A  S   LS    + +     L N   V    G ++  ++K+HLF++D+PG
Sbjct: 98  PVMERYCSLARDSNIWLSLGGFQERFDDTHLCNTHVVIDDAGMIRDTYQKMHLFDVDVPG 157

Query: 162 KITFIESKTFTAGQTPTVVDTEVGRIGIGICYDVRFPEL-AMIYAARGAHLICYPGAFNM 221
             ++ ES     G     VD+ VGR+G+ +CYD+RFP++   +   + A ++  P AF  
Sbjct: 158 GSSYKESSFTVPGTKIVSVDSPVGRLGLTVCYDLRFPKIYQQLRFEQKAQVLLVPSAFTK 217

Query: 222 TTGPLHWELLQRARAADNQLYVATCSPTRDVGADYVAWGHSTL---FGEVLATTEH--DE 281
            TG  HWE+L RARA + Q YV   +          ++G + +   +G V+         
Sbjct: 218 VTGEAHWEILLRARAIETQCYVIAAAQAGKHNEKRESYGDTLIIDPWGTVVGRLPDRVST 277

Query: 282 AIILAEIDYSLVELRRTNLPFPKQK 294
            I++A+ID+SL++  RT +P  KQ+
Sbjct: 278 GIVVADIDFSLIDSVRTKMPIDKQR 302

[Close]

BLAST of Sed0024349 vs. TAIR 10
Match: AT2G27450.1 (nitrilase-like protein 1 )

HSP 1 Score: 74.7 bits (182), Expect = 1.4e-13
Identity = 67/289 (23.18%), Postives = 119/289 (41.18%), Query Frame = 0


Query: 47  QLTATTDKQRNIEHAREAIVEAVEKGAQLVVLPELWNRPY----SNDSFPVYAEDIGASG 106
           Q   + D   N+  A   + EA  KGA ++++ EL+   Y      + F   A+      
Sbjct: 16  QFACSDDISTNVAAAERLVREAHAKGANIILIQELFEGYYFCQAQREDFFKRAKPY---- 75

Query: 107 DASPSTAMLSEVSRRL---------KRSGDKLYNACCVFGTDGKLKAKHRKIHLFNIDIP 166
              P+ A + ++++ L         + +    YN+  +   DG     +RK H     IP
Sbjct: 76  KNHPTIARMQKLAKELGVVIPVSFFEEANTAHYNSIAIIDADGTDLGIYRKSH-----IP 135

Query: 167 GKITFIESKTFTAGQTP-TVVDTEVGRIGIGICYDVRFPELAMIYAARGAHLICYPGAFN 226
               + E   F  G T   V  T+  +IG+ IC+D  FPE A     +GA ++ YP A  
Sbjct: 136 DGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTAIG 195

Query: 227 MTTGPL------HWELLQRARAADNQLYVATCSPTRDVGADYVAWGH--------STLF- 286
                       HW  + +  A  N   V     +  +G + +   H         T F 
Sbjct: 196 SEPQDQGLDSRDHWRRVMQGHAGAN---VVPLVASNRIGKEIIETEHGPSQITFYGTSFI 255

Query: 287 ----GEVLA-TTEHDEAIILAEIDYSLVELRRTNLPFPKQKRGDLYQLV 302
               GE++A   +  EA+++A+ D  +++ +R +    + +R DLY+++
Sbjct: 256 AGPTGEIVAEADDKSEAVLVAQFDLDMIKSKRQSWGVFRDRRPDLYKVL 292

[Close]

BLAST of Sed0024349 vs. TAIR 10
Match: AT2G27450.2 (nitrilase-like protein 1 )

HSP 1 Score: 68.6 bits (166), Expect = 1.0e-11
Identity = 63/269 (23.42%), Postives = 112/269 (41.64%), Query Frame = 0


Query: 67  EAVEKGAQLVVLPELWNRPY----SNDSFPVYAEDIGASGDASPSTAMLSEVSRRL---- 126
           EA  KGA ++++ EL+   Y      + F   A+         P+ A + ++++ L    
Sbjct: 63  EAHAKGANIILIQELFEGYYFCQAQREDFFKRAKPY----KNHPTIARMQKLAKELGVVI 122

Query: 127 -----KRSGDKLYNACCVFGTDGKLKAKHRKIHLFNIDIPGKITFIESKTFTAGQTP-TV 186
                + +    YN+  +   DG     +RK H     IP    + E   F  G T   V
Sbjct: 123 PVSFFEEANTAHYNSIAIIDADGTDLGIYRKSH-----IPDGPGYQEKFYFNPGDTGFKV 182

Query: 187 VDTEVGRIGIGICYDVRFPELAMIYAARGAHLICYPGAFNMTTGPL------HWELLQRA 246
             T+  +IG+ IC+D  FPE A     +GA ++ YP A              HW  + + 
Sbjct: 183 FQTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSRDHWRRVMQG 242

Query: 247 RAADNQLYVATCSPTRDVGADYVAWGH--------STLF-----GEVLA-TTEHDEAIIL 302
            A  N   V     +  +G + +   H         T F     GE++A   +  EA+++
Sbjct: 243 HAGAN---VVPLVASNRIGKEIIETEHGPSQITFYGTSFIAGPTGEIVAEADDKSEAVLV 302

The following BLAST results are available for this feature:

BLAST of Sed0024349 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6608344.1	6.9e-143	87.41	Omega-amidase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >K...	[more]
XP_022940145.1	6.9e-143	87.41	omega-amidase, chloroplastic [Cucurbita moschata] >XP_022940146.1 omega-amidase,...	[more]
XP_022134239.1	3.4e-142	86.39	omega-amidase, chloroplastic [Momordica charantia]	[more]
XP_023524248.1	4.5e-142	87.07	omega-amidase, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023524249.1 omega-...	[more]
XP_022982376.1	2.2e-141	86.73	omega-amidase, chloroplastic [Cucurbita maxima] >XP_022982377.1 omega-amidase, c...	[more]

BLAST of Sed0024349 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RUF8	5.7e-132	76.59	Omega-amidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NLP3 PE=1 SV=1	[more]
Q6IR61	3.0e-72	50.36	Omega-amidase NIT2-A OS=Xenopus laevis OX=8355 GN=nit2a PE=2 SV=1	[more]
Q10166	6.6e-72	49.27	Hydrolase C26A3.11 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284...	[more]
Q497B0	1.1e-71	50.72	Omega-amidase NIT2 OS=Rattus norvegicus OX=10116 GN=Nit2 PE=1 SV=1	[more]
Q2T9R6	1.5e-71	51.65	Omega-amidase NIT2 OS=Bos taurus OX=9913 GN=NIT2 PE=2 SV=1	[more]

BLAST of Sed0024349 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FPQ5	3.4e-143	87.41	omega-amidase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445858 PE=4 ...	[more]
A0A6J1BY88	1.7e-142	86.39	omega-amidase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006541 PE=3...	[more]
A0A6J1IZ64	1.1e-141	86.73	omega-amidase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111481222 PE=4 SV...	[more]
A0A1S3BUK3	1.3e-134	81.63	omega-amidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493689 PE=3 SV=1	[more]
A0A0A0L5D9	6.3e-134	81.29	CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G64640...	[more]

BLAST of Sed0024349 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G12040.1	4.1e-133	76.59	Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein	[more]
AT5G12040.2	7.2e-98	76.47	Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein	[more]
AT4G08790.1	1.5e-26	28.30	Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein	[more]
AT2G27450.1	1.4e-13	23.18	nitrilase-like protein 1	[more]
AT2G27450.2	1.0e-11	23.42	nitrilase-like protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003010	Carbon-nitrogen hydrolase	PFAM	PF00795	CN_hydrolase	coord: 42..282 e-value: 4.0E-48 score: 164.0
IPR003010	Carbon-nitrogen hydrolase	PROSITE	PS50263	CN_HYDROLASE	coord: 41..277 score: 51.458332
IPR036526	Carbon-nitrogen hydrolase superfamily	GENE3D	3.60.110.10		coord: 38..303 e-value: 1.5E-80 score: 272.2
IPR036526	Carbon-nitrogen hydrolase superfamily	SUPERFAMILY	56317	Carbon-nitrogen hydrolase	coord: 38..289
None	No IPR available	PANTHER	PTHR23088	NITRILASE-RELATED	coord: 29..308
None	No IPR available	PANTHER	PTHR23088:SF54	OMEGA-AMIDASE, CHLOROPLASTIC-LIKE	coord: 29..308
None	No IPR available	CDD	cd07572	nit	coord: 42..294 e-value: 2.64039E-129 score: 367.137
IPR001110	Uncharacterised protein family UPF0012, conserved site	PROSITE	PS01227	UPF0012	coord: 181..201

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0024349.1	Sed0024349.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006528	asparagine metabolic process
biological_process	GO:0006541	glutamine metabolic process
biological_process	GO:0006108	malate metabolic process
biological_process	GO:0006107	oxaloacetate metabolic process
biological_process	GO:0006807	nitrogen compound metabolic process
molecular_function	GO:0050152	omega-amidase activity