Sed0024343 (gene) Chayote v1

Overview


Name	Sed0024343
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Cytochrome P450
Location	LG13: 22942308 .. 22942844 (-)
RNA-Seq Expression	Sed0024343
Synteny	Sed0024343

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAGGCCTGGACCGAGATAAACGAGTCCGTCGGAGACCAACGCCTCGTCGAAGAAGCCGATCTCAACAAGCTACACTACCTTAAAGCCATAATCAACGAGACGTACCGGTTGTTTCCAGCCCTACCGGTATTGATTGCGCGAGAGTCTATAGAAGACTGCACAATCGGAGGCTACAACGTACCAAAAGGAACCATGTTATTAGTGAATGCATGGACGATCCATCGAGACCCAAAAGTGTGGCCCGATCCCACTTGCTTTCGACCGGAACGGTTTGGAGAGGGGGAAACCATGGAAGCGAGTAAATTGTTGTCGTTTGGAATGGGGAGAAGGGCTTGTCCTGGAGGTGGGCTAGCCTACAGGGTGGTGGCATTGGCGCTGGCTAGTATGATTCAGTGCTTTGAGTGGGAAAGGCTGGACCAAAAGGAAGTGGATTTGTCTCAAGGAATTGGACTCACCATGCCAAAAGCTAGCCCTTTGGTGGCTATGTGCAAACCTCGTAATTCCATGCTTCATTTACTATCCCAACTTTGA

mRNA sequence

ATGAAGAAGGCCTGGACCGAGATAAACGAGTCCGTCGGAGACCAACGCCTCGTCGAAGAAGCCGATCTCAACAAGCTACACTACCTTAAAGCCATAATCAACGAGACGTACCGGTTGTTTCCAGCCCTACCGGTATTGATTGCGCGAGAGTCTATAGAAGACTGCACAATCGGAGGCTACAACGTACCAAAAGGAACCATGTTATTAGTGAATGCATGGACGATCCATCGAGACCCAAAAGTGTGGCCCGATCCCACTTGCTTTCGACCGGAACGGTTTGGAGAGGGGGAAACCATGGAAGCGAGTAAATTGTTGTCGTTTGGAATGGGGAGAAGGGCTTGTCCTGGAGGTGGGCTAGCCTACAGGGTGGTGGCATTGGCGCTGGCTAGTATGATTCAGTGCTTTGAGTGGGAAAGGCTGGACCAAAAGGAAGTGGATTTGTCTCAAGGAATTGGACTCACCATGCCAAAAGCTAGCCCTTTGGTGGCTATGTGCAAACCTCGTAATTCCATGCTTCATTTACTATCCCAACTTTGA

Coding sequence (CDS)

ATGAAGAAGGCCTGGACCGAGATAAACGAGTCCGTCGGAGACCAACGCCTCGTCGAAGAAGCCGATCTCAACAAGCTACACTACCTTAAAGCCATAATCAACGAGACGTACCGGTTGTTTCCAGCCCTACCGGTATTGATTGCGCGAGAGTCTATAGAAGACTGCACAATCGGAGGCTACAACGTACCAAAAGGAACCATGTTATTAGTGAATGCATGGACGATCCATCGAGACCCAAAAGTGTGGCCCGATCCCACTTGCTTTCGACCGGAACGGTTTGGAGAGGGGGAAACCATGGAAGCGAGTAAATTGTTGTCGTTTGGAATGGGGAGAAGGGCTTGTCCTGGAGGTGGGCTAGCCTACAGGGTGGTGGCATTGGCGCTGGCTAGTATGATTCAGTGCTTTGAGTGGGAAAGGCTGGACCAAAAGGAAGTGGATTTGTCTCAAGGAATTGGACTCACCATGCCAAAAGCTAGCCCTTTGGTGGCTATGTGCAAACCTCGTAATTCCATGCTTCATTTACTATCCCAACTTTGA

Protein sequence

MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGYNVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLAYRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLLSQL

Homology

[Close]

BLAST of Sed0024343 vs. NCBI nr
Match: XP_023523499.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 307.0 bits (785), Expect = 1.1e-79
Identity = 143/178 (80.34%), Postives = 159/178 (89.33%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           MKKAWTEINE VGD+RLVEE DLN+LHYL+ IINETYRLFPALP+L+ RES EDCTIGG+
Sbjct: 334 MKKAWTEINEHVGDERLVEEMDLNQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGF 393

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLA 120
           +VPKGTML+VNAW IHRDPKVW DP  FRPERF EGE++E SKLL FGMGRRACPG GLA
Sbjct: 394 DVPKGTMLVVNAWAIHRDPKVWEDPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLA 453

Query: 121 YRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLLSQL 179
           +RVV LALA++IQCFEWERLD KEVDL+ GIGLTMPKASPLVAM KPR  ++HLLSQL
Sbjct: 454 HRVVGLALATLIQCFEWERLDSKEVDLTPGIGLTMPKASPLVAMFKPRTPIIHLLSQL 511

[Close]

BLAST of Sed0024343 vs. NCBI nr
Match: KAG7037779.1 (Cytochrome P450 81E8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 306.2 bits (783), Expect = 1.8e-79
Identity = 143/178 (80.34%), Postives = 159/178 (89.33%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           MKKAWTEINE VGD+RLVEE DLN+LHYL+ IINETYRLFPALP+L+ RES EDCTIGG+
Sbjct: 348 MKKAWTEINEHVGDERLVEEMDLNQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGF 407

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLA 120
           +VPKGTML+VNAW IHRDPKVW DP  FRPERF EGE++E SKLL FGMGRRACPG GLA
Sbjct: 408 DVPKGTMLVVNAWAIHRDPKVWEDPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLA 467

Query: 121 YRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLLSQL 179
           +RVV LALA++IQCFEWERLD KEVDL+ GIGLTMPKASPLVAM KPR  ++HLLSQL
Sbjct: 468 HRVVGLALATLIQCFEWERLDNKEVDLTPGIGLTMPKASPLVAMFKPRAPIIHLLSQL 525

[Close]

BLAST of Sed0024343 vs. NCBI nr
Match: XP_022940149.1 (cytochrome P450 81E8-like [Cucurbita moschata])

HSP 1 Score: 304.3 bits (778), Expect = 6.9e-79
Identity = 142/178 (79.78%), Postives = 158/178 (88.76%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           MKKAWTEINE VGD+RLVEE DLN+LHYL+ IINETYRLFPALP+L+ RES EDCTIGG+
Sbjct: 334 MKKAWTEINEHVGDERLVEEMDLNQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGF 393

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLA 120
           +VPKGTML+VNAW IHRDPKVW DP  FRPERF EGE++E SKLL FGMGRRACPG GLA
Sbjct: 394 DVPKGTMLVVNAWAIHRDPKVWEDPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLA 453

Query: 121 YRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLLSQL 179
           +RVV LALA++IQCFEWERLD KEVDL+ GIGLTMPKA PLVAM KPR  ++HLLSQL
Sbjct: 454 HRVVGLALATLIQCFEWERLDNKEVDLTPGIGLTMPKARPLVAMFKPRAPIIHLLSQL 511

[Close]

BLAST of Sed0024343 vs. NCBI nr
Match: XP_022982228.1 (isoflavone 3'-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 300.8 bits (769), Expect = 7.7e-78
Identity = 141/178 (79.21%), Postives = 157/178 (88.20%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           MKKAWTEINE VGD+RLVEE DLN+LHYL+ IINETYRLFPALP+L+ RES EDCTIGG+
Sbjct: 335 MKKAWTEINEHVGDERLVEEMDLNQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGF 394

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLA 120
           +VP GTML+VNAW IHRDPKVW DP  FRPERF EGE++E SKLL FGMGRRACPG GLA
Sbjct: 395 DVPIGTMLVVNAWAIHRDPKVWEDPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLA 454

Query: 121 YRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLLSQL 179
           +RVV LALA++IQCFEWERLD KEVDL+ GIGLTMPK+SPLVAM KPR  + HLLSQL
Sbjct: 455 HRVVGLALATLIQCFEWERLDNKEVDLTPGIGLTMPKSSPLVAMFKPRAPITHLLSQL 512

[Close]

BLAST of Sed0024343 vs. NCBI nr
Match: XP_004141394.1 (cytochrome P450 81Q32 [Cucumis sativus] >KGN55244.1 hypothetical protein Csa_012467 [Cucumis sativus])

HSP 1 Score: 295.4 bits (755), Expect = 3.2e-76
Identity = 135/179 (75.42%), Postives = 158/179 (88.27%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           ++KAWTEIN+ VGDQRL+ EAD NKLHYL+AIINETYRLFPALP+L+ RES EDCTIGG+
Sbjct: 345 LEKAWTEINQVVGDQRLIREADTNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGF 404

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLA 120
           ++PKGTML+VNAW +HRDP VW DP CF+PERF +GET+E +KLL FGMGRRACPG GLA
Sbjct: 405 DIPKGTMLVVNAWAVHRDPNVWEDPNCFKPERFKQGETVEVNKLLPFGMGRRACPGAGLA 464

Query: 121 YRVVALALASMIQCFEWERLD-QKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLLSQL 179
           +RVV+LALA++IQCFEW +LD QK+VDL  GIGLTMPKA PL AMCKPR S+ HLLSQL
Sbjct: 465 HRVVSLALATLIQCFEWGKLDNQKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 523

[Close]

BLAST of Sed0024343 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 6.3e-59
Identity = 104/177 (58.76%), Postives = 136/177 (76.84%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           ++KA TEI   VG  RL+EE DL KL YL  II+ET+RL PA P+L+  ES +DC + GY
Sbjct: 322 LEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGY 381

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLA 120
           +VPKGT+LLVNAW IHRDP+ W +PT F+PER G G  +E SKL+ FGMGRR+CPG GLA
Sbjct: 382 DVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHG-GVELEPSKLMPFGMGRRSCPGSGLA 441

Query: 121 YRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLLSQ 178
            RVV L L ++IQCFEW+R+ + ++D+++G GLTMPKA PL A+CKPRN +  ++S+
Sbjct: 442 QRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQPLEALCKPRNILHKVVSE 497

[Close]

BLAST of Sed0024343 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.2e-54
Identity = 102/176 (57.95%), Postives = 129/176 (73.30%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           + KA  EI+  VG  RL+EEADL++L YLK I+ ET RL PA P+L+   + EDC IG Y
Sbjct: 329 ISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSY 388

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLA 120
           ++P+GT LLVNAW IHRDP  W DP  F+PERF + E  EA KLL+FG+GRRACPG GLA
Sbjct: 389 DMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKEE--EAQKLLAFGLGRRACPGSGLA 448

Query: 121 YRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLLS 177
            R+V LAL S+IQCFEWER+   EVD+ +G+G T+PKA PL A+CK R  +  ++S
Sbjct: 449 QRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKIIS 502

[Close]

BLAST of Sed0024343 vs. ExPASy Swiss-Prot
Match: Q9SZU1 (Cytochrome P450 81F4 OS=Arabidopsis thaliana OX=3702 GN=CYP81F4 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 4.1e-50
Identity = 89/173 (51.45%), Postives = 125/173 (72.25%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           + K   EI+E +G  RL+EE+D+  L YL+ +++ET RL PA PVL+ R + ED  IGGY
Sbjct: 320 LDKVKLEIDEIIGQDRLIEESDIANLPYLQNVVSETLRLHPAAPVLVPRSTAEDIKIGGY 379

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERF--GEGETMEASKLLSFGMGRRACPGGG 120
           +VP+ TM++VNAW IHRDP +W +P  F PERF  GEGE  +   L++FG GRR CPG G
Sbjct: 380 DVPRDTMVMVNAWAIHRDPDLWTEPERFNPERFNGGEGEKDDVRMLIAFGSGRRICPGVG 439

Query: 121 LAYRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPRNSM 172
           LA+++V LAL S+IQCF+W+++++KE+D+S+G G+ M    PL A+CK R  M
Sbjct: 440 LAHKIVTLALGSLIQCFDWKKVNEKEIDMSEGPGMAMRMMVPLRALCKTRPIM 492

[Close]

BLAST of Sed0024343 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 2.7e-49
Identity = 91/169 (53.85%), Postives = 126/169 (74.56%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           ++KA TEI++ +G  RLVEE D+ KL YL+ I++ET RL+P  P+L+   + EDC + GY
Sbjct: 331 LRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGY 390

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLA 120
           +VP+GT++LVNAW IHRDPK+W +P  F+PERF   +  E  KL+ FG+GRR+CPG GLA
Sbjct: 391 DVPRGTIILVNAWAIHRDPKLWEEPEKFKPERF--EKKGEDKKLMPFGIGRRSCPGSGLA 450

Query: 121 YRVVALALASMIQCFEWERLDQKEVDLSQG-IGLTMPKASPLVAMCKPR 169
            R+V LAL S++QCFEWER+++K +D+ +   G TM KA+ L AMCK R
Sbjct: 451 QRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQAMCKAR 497

[Close]

BLAST of Sed0024343 vs. ExPASy Swiss-Prot
Match: O65790 (Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 195.7 bits (496), Expect = 4.6e-49
Identity = 89/177 (50.28%), Postives = 123/177 (69.49%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           ++KA +EI+E +G  RL++E+D+  L YL+ +++ET+RLFP  P LI R   +D  IGGY
Sbjct: 323 LEKARSEIDEKIGKDRLIDESDIAVLPYLQNVVSETFRLFPVAPFLIPRSPTDDMKIGGY 382

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERF--GEGETMEASKLLSFGMGRRACPGGG 120
           +VP+ T+++VNAW IHRDP++W +P  F P+R+  G G      KL+ FG GRR CPG G
Sbjct: 383 DVPRDTIVMVNAWAIHRDPEIWEEPEKFNPDRYNDGCGSDYYVYKLMPFGNGRRTCPGAG 442

Query: 121 LAYRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLL 176
           L  R+V LAL S+IQCFEWE +  +E+D+S+  GL M K  PL AMC+PR  M  LL
Sbjct: 443 LGQRIVTLALGSLIQCFEWENVKGEEMDMSESTGLGMRKMDPLRAMCRPRPIMSKLL 499

[Close]

BLAST of Sed0024343 vs. ExPASy TrEMBL
Match: A0A6J1FJ78 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111445861 PE=3 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 3.4e-79
Identity = 142/178 (79.78%), Postives = 158/178 (88.76%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           MKKAWTEINE VGD+RLVEE DLN+LHYL+ IINETYRLFPALP+L+ RES EDCTIGG+
Sbjct: 334 MKKAWTEINEHVGDERLVEEMDLNQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGF 393

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLA 120
           +VPKGTML+VNAW IHRDPKVW DP  FRPERF EGE++E SKLL FGMGRRACPG GLA
Sbjct: 394 DVPKGTMLVVNAWAIHRDPKVWEDPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLA 453

Query: 121 YRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLLSQL 179
           +RVV LALA++IQCFEWERLD KEVDL+ GIGLTMPKA PLVAM KPR  ++HLLSQL
Sbjct: 454 HRVVGLALATLIQCFEWERLDNKEVDLTPGIGLTMPKARPLVAMFKPRAPIIHLLSQL 511

[Close]

BLAST of Sed0024343 vs. ExPASy TrEMBL
Match: A0A6J1J4B3 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111481120 PE=3 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 3.7e-78
Identity = 141/178 (79.21%), Postives = 157/178 (88.20%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           MKKAWTEINE VGD+RLVEE DLN+LHYL+ IINETYRLFPALP+L+ RES EDCTIGG+
Sbjct: 335 MKKAWTEINEHVGDERLVEEMDLNQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGF 394

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLA 120
           +VP GTML+VNAW IHRDPKVW DP  FRPERF EGE++E SKLL FGMGRRACPG GLA
Sbjct: 395 DVPIGTMLVVNAWAIHRDPKVWEDPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLA 454

Query: 121 YRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLLSQL 179
           +RVV LALA++IQCFEWERLD KEVDL+ GIGLTMPK+SPLVAM KPR  + HLLSQL
Sbjct: 455 HRVVGLALATLIQCFEWERLDNKEVDLTPGIGLTMPKSSPLVAMFKPRAPITHLLSQL 512

[Close]

BLAST of Sed0024343 vs. ExPASy TrEMBL
Match: A0A0A0L2I1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641760 PE=3 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.6e-76
Identity = 135/179 (75.42%), Postives = 158/179 (88.27%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           ++KAWTEIN+ VGDQRL+ EAD NKLHYL+AIINETYRLFPALP+L+ RES EDCTIGG+
Sbjct: 345 LEKAWTEINQVVGDQRLIREADTNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGF 404

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLA 120
           ++PKGTML+VNAW +HRDP VW DP CF+PERF +GET+E +KLL FGMGRRACPG GLA
Sbjct: 405 DIPKGTMLVVNAWAVHRDPNVWEDPNCFKPERFKQGETVEVNKLLPFGMGRRACPGAGLA 464

Query: 121 YRVVALALASMIQCFEWERLD-QKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLLSQL 179
           +RVV+LALA++IQCFEW +LD QK+VDL  GIGLTMPKA PL AMCKPR S+ HLLSQL
Sbjct: 465 HRVVSLALATLIQCFEWGKLDNQKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 523

[Close]

BLAST of Sed0024343 vs. ExPASy TrEMBL
Match: A0A5D3D9B5 (Isoflavone 2'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002940 PE=3 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 2.2e-75
Identity = 134/179 (74.86%), Postives = 157/179 (87.71%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           M KAWTEIN+ VGDQRLV EAD N LHYL++IINETYRLFPALP+L+ RES EDCTIGG+
Sbjct: 344 MNKAWTEINQVVGDQRLVREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGF 403

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLA 120
           ++PKGTML+VNAW +HR+P+VW DP CF+PERF +GET+E SKLL FGMGRRACPG GLA
Sbjct: 404 DIPKGTMLVVNAWAVHRNPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLA 463

Query: 121 YRVVALALASMIQCFEWERLD-QKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLLSQL 179
           +RVV+LALA++IQCFEW +LD +K+VDL  GIGLTMPKA PL AMCKPR S+ HLLSQL
Sbjct: 464 HRVVSLALATLIQCFEWGKLDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522

[Close]

BLAST of Sed0024343 vs. ExPASy TrEMBL
Match: A0A1S3BTN1 (isoflavone 2'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103493583 PE=3 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 2.2e-75
Identity = 134/179 (74.86%), Postives = 157/179 (87.71%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           M KAWTEIN+ VGDQRLV EAD N LHYL++IINETYRLFPALP+L+ RES EDCTIGG+
Sbjct: 344 MNKAWTEINQVVGDQRLVREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGF 403

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLA 120
           ++PKGTML+VNAW +HR+P+VW DP CF+PERF +GET+E SKLL FGMGRRACPG GLA
Sbjct: 404 DIPKGTMLVVNAWAVHRNPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLA 463

Query: 121 YRVVALALASMIQCFEWERLD-QKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLLSQL 179
           +RVV+LALA++IQCFEW +LD +K+VDL  GIGLTMPKA PL AMCKPR S+ HLLSQL
Sbjct: 464 HRVVSLALATLIQCFEWGKLDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522

[Close]

BLAST of Sed0024343 vs. TAIR 10
Match: AT1G66540.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 215.3 bits (547), Expect = 3.9e-56
Identity = 100/169 (59.17%), Postives = 132/169 (78.11%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           +KK   EI+  +G  RL+EE+D+  L YL+ I++ET RL+PA P+L+   S EDC +GGY
Sbjct: 210 LKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLRLYPAGPLLVPHISSEDCKVGGY 269

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERF-GEGETMEASKLLSFGMGRRACPGGGL 120
           ++P GTMLLVN W IHRDP++W DP  F+PERF  EGET    KLL+FG+GRRACPG GL
Sbjct: 270 DMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFEKEGET---HKLLTFGLGRRACPGSGL 329

Query: 121 AYRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPR 169
           A R+V+L+L S+IQCFEWER+ ++EVD+++G GLTMP+A PLVAMC+ R
Sbjct: 330 ARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLTMPRAIPLVAMCRAR 375

[Close]

BLAST of Sed0024343 vs. TAIR 10
Match: AT1G66540.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 215.3 bits (547), Expect = 3.9e-56
Identity = 100/169 (59.17%), Postives = 132/169 (78.11%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           +KK   EI+  +G  RL+EE+D+  L YL+ I++ET RL+PA P+L+   S EDC +GGY
Sbjct: 151 LKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLRLYPAGPLLVPHISSEDCKVGGY 210

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERF-GEGETMEASKLLSFGMGRRACPGGGL 120
           ++P GTMLLVN W IHRDP++W DP  F+PERF  EGET    KLL+FG+GRRACPG GL
Sbjct: 211 DMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFEKEGET---HKLLTFGLGRRACPGSGL 270

Query: 121 AYRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPR 169
           A R+V+L+L S+IQCFEWER+ ++EVD+++G GLTMP+A PLVAMC+ R
Sbjct: 271 ARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLTMPRAIPLVAMCRAR 316

[Close]

BLAST of Sed0024343 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 214.2 bits (544), Expect = 8.8e-56
Identity = 98/170 (57.65%), Postives = 131/170 (77.06%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           + KA  EI+  +G  RL+EE+D+  L YL+ I++ET RL+PA P+L+   + +DC +GGY
Sbjct: 323 LNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGY 382

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERF-GEGETMEASKLLSFGMGRRACPGGGL 120
           ++P+GTMLL NAW IHRDP +W DPT F+PERF  EG   EA KL+ FG+GRRACPG GL
Sbjct: 383 DMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEG---EAKKLMPFGLGRRACPGSGL 442

Query: 121 AYRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPRN 170
           A R+V L+L S+IQCFEWER+ ++EVD+++G GLTMPKA PL AMC+ R+
Sbjct: 443 AQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEAMCRARD 489

[Close]

BLAST of Sed0024343 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 214.2 bits (544), Expect = 8.8e-56
Identity = 102/176 (57.95%), Postives = 129/176 (73.30%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           + KA  EI+  VG  RL+EEADL++L YLK I+ ET RL PA P+L+   + EDC IG Y
Sbjct: 329 ISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSY 388

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERFGEGETMEASKLLSFGMGRRACPGGGLA 120
           ++P+GT LLVNAW IHRDP  W DP  F+PERF + E  EA KLL+FG+GRRACPG GLA
Sbjct: 389 DMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKEE--EAQKLLAFGLGRRACPGSGLA 448

Query: 121 YRVVALALASMIQCFEWERLDQKEVDLSQGIGLTMPKASPLVAMCKPRNSMLHLLS 177
            R+V LAL S+IQCFEWER+   EVD+ +G+G T+PKA PL A+CK R  +  ++S
Sbjct: 449 QRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKIIS 502

[Close]

BLAST of Sed0024343 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 213.8 bits (543), Expect = 1.1e-55
Identity = 102/179 (56.98%), Postives = 136/179 (75.98%), Query Frame = 0


Query: 1   MKKAWTEINESVGDQRLVEEADLNKLHYLKAIINETYRLFPALPVLIARESIEDCTIGGY 60
           + KA  EI+  VG  RLVEE+DL+ L YL+ I++E+ RL+PA P+L+   + EDC +GGY
Sbjct: 323 LSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGY 382

Query: 61  NVPKGTMLLVNAWTIHRDPKVWPDPTCFRPERF-GEGETMEASKLLSFGMGRRACPGGGL 120
           ++P+GTMLL NAW IHRDPK+W DPT F+PERF  EG   EA KLL FG+GRRACPG GL
Sbjct: 383 HMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKEG---EAQKLLGFGLGRRACPGSGL 442

Query: 121 AYRVVALALASMIQCFEWERLDQKEVDLSQ-GIGLTMPKASPLVAMCKPRNSMLHLLSQ 178
           A R+ +L + S+IQCFEWER+ ++EVD+++ G G+ MPKA PLVAMCK R  +  +L++
Sbjct: 443 AQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAIPLVAMCKARPVVGKILNE 498

The following BLAST results are available for this feature:

BLAST of Sed0024343 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023523499.1	1.1e-79	80.34	cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo]	[more]
KAG7037779.1	1.8e-79	80.34	Cytochrome P450 81E8 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022940149.1	6.9e-79	79.78	cytochrome P450 81E8-like [Cucurbita moschata]	[more]
XP_022982228.1	7.7e-78	79.21	isoflavone 3'-hydroxylase-like [Cucurbita maxima]	[more]
XP_004141394.1	3.2e-76	75.42	cytochrome P450 81Q32 [Cucumis sativus] >KGN55244.1 hypothetical protein Csa_012...	[more]

BLAST of Sed0024343 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	6.3e-59	58.76	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q9FG65	1.2e-54	57.95	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]
Q9SZU1	4.1e-50	51.45	Cytochrome P450 81F4 OS=Arabidopsis thaliana OX=3702 GN=CYP81F4 PE=1 SV=1	[more]
Q9LHA1	2.7e-49	53.85	Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1	[more]
O65790	4.6e-49	50.28	Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2	[more]

BLAST of Sed0024343 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FJ78	3.4e-79	79.78	cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111445861 PE=3 SV=...	[more]
A0A6J1J4B3	3.7e-78	79.21	isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111481120 PE=3 ...	[more]
A0A0A0L2I1	1.6e-76	75.42	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641760 PE=3 SV=1	[more]
A0A5D3D9B5	2.2e-75	74.86	Isoflavone 2'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3BTN1	2.2e-75	74.86	isoflavone 2'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103493583 PE=3 SV=1	[more]

BLAST of Sed0024343 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G66540.1	3.9e-56	59.17	Cytochrome P450 superfamily protein	[more]
AT1G66540.2	3.9e-56	59.17	Cytochrome P450 superfamily protein	[more]
AT4G37360.1	8.8e-56	57.65	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT5G36220.1	8.8e-56	57.95	cytochrome p450 81d1	[more]
AT4G37340.1	1.1e-55	56.98	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 31..42 score: 41.42 coord: 105..114 score: 52.75 coord: 114..125 score: 33.24
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..157 e-value: 2.1E-46 score: 158.7
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 114..137 score: 34.0 coord: 71..95 score: 39.67 coord: 30..48 score: 33.95 coord: 104..114 score: 50.08
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..171 e-value: 4.9E-56 score: 192.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 1..168
None	No IPR available	PANTHER	PTHR47947:SF27	SUBFAMILY NOT NAMED	coord: 2..176
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 2..176
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 107..116

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0024343.1	Sed0024343.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen