Sed0024037 (gene) Chayote v1

Overview


Name	Sed0024037
Type	gene
Organism	Sechium edule (Chayote v1)
Description	gibberellin 3-beta-dioxygenase 1-like
Location	LG01: 9953099 .. 9954896 (+)
RNA-Seq Expression	Sed0024037
Synteny	Sed0024037

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGATCAAAAGCTTCCTCCCCCAAGCCAACTTGTTCCTCTCGACTTCAGCTCAATTCAAACTGTTCCTAAATCTCATGTTTGGCTCGACTCCGATGAGCCATCCTCAGCGATCTGCTTGGACGAAAAGATTTCGCTCCCATTGATCGATCTTCTTGATGACCGAGCATCAGAGCTTCTAGGGAAGGCTTGTGAGAAATGGGGAATGTTTCAGTTGATTAACCATGGTGTCCCCAAAACCCTAATAGCCGAAACCGAGGAAGAGACTCAACGTCTATTCACTCTTCCAACAAGAGATAAGATGAAGACATTACAGGCTCCCGACAACATCACCGGTTACGGCATGGCCGTAATATCGAAACATTATTCGAAATACATGTGGCATGAAGGATTCACCATCTTGGGTTCTCCGATTGATGATGCTAAGAAGCTTTGGCCATCTAACTACAAACGGTTTTGGTAAATTTCATGCACTTTCATGCACGATACAATTAGGGTTTATCGCTATAAAAATATAAAATCTATTTGGTAGCAAGACAGTTCATCAACATATACGGTACATAAGAAAAAATGTATAAAAAATAAGTTCCTTTGAATGAATAGATTCGGTCGCAATTTTAAAATATGTAATTCAAAAAAGAAATAATACTCTTAATTAATTAACCATAGAGTTACATTGAAAAATACAATCAACGAATATTTATCATGTTTGAAAAAGGTTAGAAATTGAGTAAATTTCATGCATTTTTTGGAATAAAAAATTACCTTAATAATTTCCTTCATTATAATTTAGTGTTGATTTTTATTTTGTCGATTCTGATTATTTAAGTATGTAGTAAAATTTGATTCTTCTATACACAACTCTTCATAAAAGCCATTTAGATGCTTTTGACAGAGTTTGCTTCTTTCTTTTATATATATATATATATATATATATTTAGTTCACGAATATTTTGTAAATAGAAGATTCCACCTAAAACCTCTAAATAATAAATACATATTTTATATTGATTGAACTATGTTCTTGTGGGTAAAATTGACTAAATTATTTGACTATGGCAAACCTTAAAAGGTTAACGGATTAAATTTCATTTGTAATTCGATTATACCCTTCATTAATTTATTTTTTATGTTAAATTTTATAAATGAGTTAATGATCACAATCATATAACTAAACATTTTATCATCTAAAATTTTCTACTGATTGTTGTAGTGAGGTAATGGAAGAGTATCAAACCAAAATGAAGGGTTTAGTAGAAATGTTACTAAGGTTAACCTTCAAGTTTCTCGACATCTCCGAGGAAGAGATAACGAATGTGTTGTCTTCGATCGAAAACTCTGGCAAGCCCAACACCGCTCTCCGCCTGAACTCCTATCCACCGTGCCCCGATCCGGATCAAGTCATGGGCCTCGCTGCCCACACCGACACCTCCCTCTTCACCATCGTTCACCAGGCATGCAATGATGGCCTCCAAATTTTTAACGACGGAGTAGGTTGGGTTCCGTTGTCTCCAATGAGCGGTACTCTCATCGTCAATGTCGGTGATCTCCTGCACATTATATCGAATGGGAGGTTTCGGAGCATTCTTCATCGGGTGATGATACGAAAGAAGGCTGAGCATAGATTTTCTTTGGCATATTTCTATAGTCCGCCGGCTGATTTATACATTTCACCTTATTGCAAGCCATTGAGTAAATCTCAACAGATTCCCATTTATCGCAGTGTGACTGTGAAAGAATATACCACCATAAAGGCCAAGAACAATGCGGATGGACTTTCTATCATAAAAATGTGA

mRNA sequence

ATGGCAGATCAAAAGCTTCCTCCCCCAAGCCAACTTGTTCCTCTCGACTTCAGCTCAATTCAAACTGTTCCTAAATCTCATGTTTGGCTCGACTCCGATGAGCCATCCTCAGCGATCTGCTTGGACGAAAAGATTTCGCTCCCATTGATCGATCTTCTTGATGACCGAGCATCAGAGCTTCTAGGGAAGGCTTGTGAGAAATGGGGAATGTTTCAGTTGATTAACCATGGTGTCCCCAAAACCCTAATAGCCGAAACCGAGGAAGAGACTCAACGTCTATTCACTCTTCCAACAAGAGATAAGATGAAGACATTACAGGCTCCCGACAACATCACCGGTTACGGCATGGCCGTAATATCGAAACATTATTCGAAATACATGTGGCATGAAGGATTCACCATCTTGGGTTCTCCGATTGATGATGCTAAGAAGCTTTGGCCATCTAACTACAAACGGTTTTGTGAGGTAATGGAAGAGTATCAAACCAAAATGAAGGGTTTAGTAGAAATGTTACTAAGGTTAACCTTCAAGTTTCTCGACATCTCCGAGGAAGAGATAACGAATGTGTTGTCTTCGATCGAAAACTCTGGCAAGCCCAACACCGCTCTCCGCCTGAACTCCTATCCACCGTGCCCCGATCCGGATCAAGTCATGGGCCTCGCTGCCCACACCGACACCTCCCTCTTCACCATCGTTCACCAGGCATGCAATGATGGCCTCCAAATTTTTAACGACGGAGTAGGTTGGGTTCCGTTGTCTCCAATGAGCGGTACTCTCATCGTCAATGTCGGTGATCTCCTGCACATTATATCGAATGGGAGGTTTCGGAGCATTCTTCATCGGGTGATGATACGAAAGAAGGCTGAGCATAGATTTTCTTTGGCATATTTCTATAGTCCGCCGGCTGATTTATACATTTCACCTTATTGCAAGCCATTGAGTAAATCTCAACAGATTCCCATTTATCGCAGTGTGACTGTGAAAGAATATACCACCATAAAGGCCAAGAACAATGCGGATGGACTTTCTATCATAAAAATGTGA

Coding sequence (CDS)

ATGGCAGATCAAAAGCTTCCTCCCCCAAGCCAACTTGTTCCTCTCGACTTCAGCTCAATTCAAACTGTTCCTAAATCTCATGTTTGGCTCGACTCCGATGAGCCATCCTCAGCGATCTGCTTGGACGAAAAGATTTCGCTCCCATTGATCGATCTTCTTGATGACCGAGCATCAGAGCTTCTAGGGAAGGCTTGTGAGAAATGGGGAATGTTTCAGTTGATTAACCATGGTGTCCCCAAAACCCTAATAGCCGAAACCGAGGAAGAGACTCAACGTCTATTCACTCTTCCAACAAGAGATAAGATGAAGACATTACAGGCTCCCGACAACATCACCGGTTACGGCATGGCCGTAATATCGAAACATTATTCGAAATACATGTGGCATGAAGGATTCACCATCTTGGGTTCTCCGATTGATGATGCTAAGAAGCTTTGGCCATCTAACTACAAACGGTTTTGTGAGGTAATGGAAGAGTATCAAACCAAAATGAAGGGTTTAGTAGAAATGTTACTAAGGTTAACCTTCAAGTTTCTCGACATCTCCGAGGAAGAGATAACGAATGTGTTGTCTTCGATCGAAAACTCTGGCAAGCCCAACACCGCTCTCCGCCTGAACTCCTATCCACCGTGCCCCGATCCGGATCAAGTCATGGGCCTCGCTGCCCACACCGACACCTCCCTCTTCACCATCGTTCACCAGGCATGCAATGATGGCCTCCAAATTTTTAACGACGGAGTAGGTTGGGTTCCGTTGTCTCCAATGAGCGGTACTCTCATCGTCAATGTCGGTGATCTCCTGCACATTATATCGAATGGGAGGTTTCGGAGCATTCTTCATCGGGTGATGATACGAAAGAAGGCTGAGCATAGATTTTCTTTGGCATATTTCTATAGTCCGCCGGCTGATTTATACATTTCACCTTATTGCAAGCCATTGAGTAAATCTCAACAGATTCCCATTTATCGCAGTGTGACTGTGAAAGAATATACCACCATAAAGGCCAAGAACAATGCGGATGGACTTTCTATCATAAAAATGTGA

Protein sequence

MADQKLPPPSQLVPLDFSSIQTVPKSHVWLDSDEPSSAICLDEKISLPLIDLLDDRASELLGKACEKWGMFQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVISKHYSKYMWHEGFTILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLDISEEEITNVLSSIENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFNDGVGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSPPADLYISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIKM

Homology

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BLAST of Sed0024037 vs. NCBI nr
Match: AAF80661.1 (putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 539.7 bits (1389), Expect = 1.9e-149
Identity = 263/349 (75.36%), Postives = 300/349 (85.96%), Query Frame = 0


Query: 1   MADQKLPPPSQLVPLDFSSIQTVPKSHVWLDSDEPSSAICLDEKISLPLIDLLDDRASEL 60
           MADQ+LPPPSQLVPLDF+SIQTVP+SH+W +S E S AI LDE+ISLP+ID LDD ASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  LGKACEKWGMFQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPD-NITGYGMAVI 120
           LG+ACE+WGMFQLINHGVPKTLI E EEET+RLF+LP R KMKTL+      TGYGMA I
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120

Query: 121 SKHYSKYMWHEGFTILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFL 180
           SK++ K MWHEGFT+ GSP+DDAKKLWP++YKRFC+VMEEYQ++MKGL E ++RL FKFL
Sbjct: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180

Query: 181 DISEEEITNVLSSI-ENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACND 240
           DISEEE+  +L+S  E++GKP  ALRLNSYPPCPDP +VMGLAAHTDTSLFTIVHQA +D
Sbjct: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240

Query: 241 GLQIFNDGVGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFY 300
           GLQ+F DG GWVPLSPMSGTLIVNVGD+LHI+SNGRF SILHRVMI +  EHRFSLAYFY
Sbjct: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300

Query: 301 SPPADLYISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIKM 348
            PP DLYISPYCKPLS+S QIP+YR V VKEY TIKAK    GLS IK+
Sbjct: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

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BLAST of Sed0024037 vs. NCBI nr
Match: XP_038885582.1 (gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida])

HSP 1 Score: 535.0 bits (1377), Expect = 4.7e-148
Identity = 254/346 (73.41%), Postives = 298/346 (86.13%), Query Frame = 0


Query: 3   DQKLPPPSQLVPLDFSSIQTVPKSHVWLDSDEPSSAICLDEKISLPLIDLLDDRASELLG 62
           DQ+LPPPSQ VPLDFSSIQTVP+SH+W DS+E S A+ LDE++SLP+IDLLD+ ASELLG
Sbjct: 5   DQELPPPSQFVPLDFSSIQTVPESHLWPDSNELSLAVELDERVSLPIIDLLDNGASELLG 64

Query: 63  KACEKWGMFQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVISKH 122
           +ACE+WGMFQLINHGVPKTLI E EEET+RLFTLP R KMK LQ+P +ITGYGMA ISK+
Sbjct: 65  RACEEWGMFQLINHGVPKTLIDEAEEETRRLFTLPARQKMKALQSPSSITGYGMAGISKY 124

Query: 123 YSKYMWHEGFTILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLDIS 182
           + K MWHEGFT+LGSP+DDAKKLWP++YKRFC+VMEEYQ +MKGL E L+RL FKFL IS
Sbjct: 125 HPKLMWHEGFTVLGSPVDDAKKLWPNDYKRFCDVMEEYQNQMKGLGERLIRLIFKFLHIS 184

Query: 183 EEEITNVL-SSIENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQ 242
           ++E+  +L S+ ++ GKP  ALRLNSYPPCPDP +V+GLAAHTDTSL TI+HQ  +DGLQ
Sbjct: 185 DKEMVKMLTSTCDSGGKPYMALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQ 244

Query: 243 IFNDGVGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSPP 302
           +FNDG GWVPLSPMSGTLIVNVGD+LHI+SNGRF SILHRV I++  EHR+SL YFY PP
Sbjct: 245 VFNDGAGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPP 304

Query: 303 ADLYISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIKM 348
           A LYISPYCKPLS+S QIP+YR V V+EY TIKA N   GLS IK+
Sbjct: 305 AGLYISPYCKPLSESPQIPLYRCVNVQEYFTIKASNKGKGLSAIKI 350

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BLAST of Sed0024037 vs. NCBI nr
Match: XP_022951873.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 525.8 bits (1353), Expect = 2.9e-145
Identity = 254/347 (73.20%), Postives = 295/347 (85.01%), Query Frame = 0


Query: 1   MADQKLPPPSQLVPLDFSSIQTVPKSHVWLDSDEPSSAICLDEKISLPLIDLLDDRASEL 60
           MADQ      + +PLDF+S+QTVP+SH+W DSDE SS I LDEKISLP IDLLDD ASEL
Sbjct: 1   MADQ------EFLPLDFNSVQTVPESHLWPDSDELSSVIDLDEKISLPSIDLLDDGASEL 60

Query: 61  LGKACEKWGMFQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVIS 120
           LG+ACE+WGMFQL NHG+ KTLIAETEEE +RLFTLP ++KMKTL++ D +TGYG AVIS
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAETEEEARRLFTLPVKEKMKTLRSADRVTGYGTAVIS 120

Query: 121 KHYSKYMWHEGFTILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLD 180
           K++ K MWHEGFTILGSP DDAKKLWPS+ KRFC+VMEEYQ++MK L + L++L FKFLD
Sbjct: 121 KYHPKCMWHEGFTILGSPADDAKKLWPSDSKRFCDVMEEYQSEMKRLSDRLIKLIFKFLD 180

Query: 181 ISEEEITNVLSSIENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGL 240
           IS EE   +LSS+++SGK  TAL+LNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGL
Sbjct: 181 ISVEETMKLLSSMDDSGKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGL 240

Query: 241 QIFNDGVGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSP 300
           QIF D  GWVPLSPM G+ +VNVGDLLHI+SNGRFRSILHRV I+ K  HRFSLAYFY P
Sbjct: 241 QIFKDKAGWVPLSPMRGSFVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYP 300

Query: 301 PADLYISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIKM 348
           P  LYISP+CKPLS+S Q P+YR V+VKEY T+KAKNN +GLS +K+
Sbjct: 301 PPYLYISPHCKPLSESPQTPLYRCVSVKEYFTMKAKNNGEGLSTVKI 341

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BLAST of Sed0024037 vs. NCBI nr
Match: XP_023537854.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521.2 bits (1341), Expect = 7.0e-144
Identity = 248/337 (73.59%), Postives = 291/337 (86.35%), Query Frame = 0


Query: 11  QLVPLDFSSIQTVPKSHVWLDSDEPSSAICLDEKISLPLIDLLDDRASELLGKACEKWGM 70
           + +PLDF+S+QTVP+SH+W DSDE SS I LDEKISLP IDLLDD ASELLG+ACE+WGM
Sbjct: 5   EFLPLDFNSVQTVPESHLWPDSDELSSVIDLDEKISLPSIDLLDDGASELLGRACEQWGM 64

Query: 71  FQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVISKHYSKYMWHE 130
           FQL NHG+ KTLIA+ EEE +RLFTLP ++KMKTL++ D +TGYG AVISK++SK MWHE
Sbjct: 65  FQLTNHGISKTLIAKMEEEARRLFTLPAKEKMKTLRSADRVTGYGTAVISKYHSKCMWHE 124

Query: 131 GFTILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLDISEEEITNVL 190
           GFTILGSP DDAKKLWPS+ KRFC+VMEEYQ++MK L + L++L FKFLDISEEE   +L
Sbjct: 125 GFTILGSPADDAKKLWPSDSKRFCDVMEEYQSEMKRLSDRLIKLIFKFLDISEEETMKLL 184

Query: 191 SSIENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFNDGVGWV 250
           SS+++SGK  TAL+LNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIF D  GW+
Sbjct: 185 SSMDDSGKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWI 244

Query: 251 PLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSPPADLYISPYC 310
           PLSPM G+ +VNVGDLLHI+SNGRFRSILHRV I+ K  HRFSLAYFY PP  LYI+P+C
Sbjct: 245 PLSPMRGSFVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHC 304

Query: 311 KPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIKM 348
           K LS+S Q P+YR V+VKEY T+KAKNN +GLS IK+
Sbjct: 305 KALSESPQTPLYRCVSVKEYFTMKAKNNGEGLSTIKI 341

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BLAST of Sed0024037 vs. NCBI nr
Match: NP_001275523.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >KAE8652189.1 hypothetical protein Csa_021993 [Cucumis sativus] >CCG14217.1 GA 3-oxidase [Cucumis sativus])

HSP 1 Score: 520.0 bits (1338), Expect = 1.6e-143
Identity = 250/350 (71.43%), Postives = 297/350 (84.86%), Query Frame = 0


Query: 1   MADQKLPPPSQLVPLDFSSIQTVPKSHVWLDSDEPSSAICLDEKISLPLIDLLDDRASEL 60
           MADQ++PPPSQLVPLDF+S+QTVP+SH+W ++ E S+AI LD+++SLPLIDL+ D ASEL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  LGKACEKWGMFQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNI--TGYGMAV 120
           LG+ACE+WGMFQLINHG+ KTLI E EEET+RLFTLP   KMKTL++P ++  TGYGMA 
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 ISKHYSKYMWHEGFTILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKF 180
           ISK++ K MWHEGFTILGSPI++AKKLWP++YKRFC+VMEEYQ +MKGL E ++RL  KF
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180

Query: 181 LDISEEEITNVLSSIENS-GKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACN 240
           L ISEEE+  +L+S + S GKP+ ALRLNSYPPCPDP QVMGLAAHTDTSL TI+HQ  N
Sbjct: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240

Query: 241 DGLQIFNDGVGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYF 300
           +GLQIF DG GWVPLSPMSGTLIVN+GDLLHI+SNGRF SILHRVMI++  EHRFSLAYF
Sbjct: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300

Query: 301 YSPPADLYISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIKM 348
           Y PP ++YISPYCKPLS + Q P+YRSV VKEY  IKAK    GL  IK+
Sbjct: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

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BLAST of Sed0024037 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 7.4e-80
Identity = 146/336 (43.45%), Postives = 221/336 (65.77%), Query Frame = 0


Query: 16  DFSSIQTVPKSHVWLDSDE----PSSAICLDEKISLPLIDLLDDRASELLGKACEKWGMF 75
           DF+S+Q +P+S+ W   D+     S+ I  +   ++P+IDL D  AS+L+G AC+ WG++
Sbjct: 21  DFNSLQELPESYNWTHLDDHTLIDSNNIMKESTTTVPVIDLNDPNASKLIGLACKTWGVY 80

Query: 76  QLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVISKHYSKYMWHEG 135
           Q++NHG+P +L+ + +   Q LF+LP+  K K  ++PD ++GYG+A IS  + K MW+EG
Sbjct: 81  QVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGVSGYGIARISSFFPKLMWYEG 140

Query: 136 FTILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLDISEEEITNVLS 195
           FTI+GSP+D  ++LWP +Y RFC+++ +Y   MK L   L+ L    L I++E+I    S
Sbjct: 141 FTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLAGTLMCLMLDSLGITKEDIKWAGS 200

Query: 196 SIENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFNDGVGWVP 255
             +   K   AL+LNSYP CPDPD  MGLA HTD++  TI+ Q    GLQ+  +G GW+ 
Sbjct: 201 KAQFE-KACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILSQNDISGLQVNREGSGWIT 260

Query: 256 LSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSPPADLYISPYCK 315
           + P+ G L+VNVGDL HI+SNG + S+LHRV++  +   RFS+AY Y PP+++ I P+ K
Sbjct: 261 VPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLV-NRTRQRFSVAYLYGPPSNVEICPHAK 320

Query: 316 PLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIKM 348
            +  ++  P+YRSVT  EY   KAK+    LS +++
Sbjct: 321 LIGPTKP-PLYRSVTWNEYLGTKAKHFNKALSSVRL 353

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BLAST of Sed0024037 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 285.0 bits (728), Expect = 1.1e-75
Identity = 148/334 (44.31%), Postives = 211/334 (63.17%), Query Frame = 0


Query: 14  PLDFSSIQTVPKSHVWLDSDE-PSSAICLDEKISLPLIDLLDDRASELLGKACEKWGMFQ 73
           P DF S+   P S+ W   D+   SA   DE  +LPLIDL D   + L+G AC  WG FQ
Sbjct: 21  PPDFKSL---PDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTTWGAFQ 80

Query: 74  LINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVISKHYSKYMWHEGF 133
           + NHGVP  L+ + E  T  LF LP + K+K  ++ + ++GYG+A I+  ++K MW EGF
Sbjct: 81  ITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIASFFNKKMWSEGF 140

Query: 134 TILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLDISEEEITNVLSS 193
           T++GSP+ D +KLWPS++ ++CE++EEY+  M+ L   L+      L + E++I     +
Sbjct: 141 TVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEEKDIQWAGPN 200

Query: 194 IENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFNDGVGWVPL 253
            +  G     ++LN YP CP+PD+ MGLAAHTD++L TI++Q    GLQ+F D VGWV  
Sbjct: 201 SDFQG-TQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWVTA 260

Query: 254 SPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSPPADLYISPYCKP 313
            P+ G+L+VNVGDLLHI++NG F S+LHR  +      RFS+AY + PP+D+ ISP  K 
Sbjct: 261 PPVPGSLVVNVGDLLHILTNGIFPSVLHRARV-NHVRSRFSMAYLWGPPSDIMISPLPK- 320

Query: 314 LSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIK 347
           L    Q P+Y S+T K+Y   KA +    LSII+
Sbjct: 321 LVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of Sed0024037 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 280.0 bits (715), Expect = 3.6e-74
Identity = 149/345 (43.19%), Postives = 218/345 (63.19%), Query Frame = 0


Query: 9   PSQLVPLDFSSIQTVPKSHVWLDSDE------PSSAICLDEKISLPLIDLLDDRASELLG 68
           P   +P DF+S++ +P S+ W   D+      PS     +   ++PLIDL    A+  +G
Sbjct: 17  PHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGE---NIPLIDLDHPDATNQIG 76

Query: 69  KACEKWGMFQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVISKH 128
            AC  WG FQ+ NHGVP  L+ + E  T  LF LP + K+K+ ++   ++GYG+A I+  
Sbjct: 77  HACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGYGVARIASF 136

Query: 129 YSKYMWHEGFTILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLDIS 188
           ++K MW EGFTI GSP++D +KLWP ++  +C+++EEY+  MK L   L+ L    L +S
Sbjct: 137 FNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVS 196

Query: 189 EEEIT-NVLSSIENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQ 248
           EE+I    LSS  N  +   AL+LN YP CP+PD+ MGLAAHTD++L TI++Q    GLQ
Sbjct: 197 EEDIEWASLSSDLNWAQ--AALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQ 256

Query: 249 IFNDGVGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSPP 308
           +F D +GWV + P  G+L+VNVGDL HI+SNG F+S+LHR  +  +   R S+A+ + P 
Sbjct: 257 VFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARV-NQTRARLSVAFLWGPQ 316

Query: 309 ADLYISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIK 347
           +D+ ISP  K +S  +  P+Y+SVT KEY   KA +    LS+I+
Sbjct: 317 SDIKISPVPKLVSPVES-PLYQSVTWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of Sed0024037 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.7e-66
Identity = 136/342 (39.77%), Postives = 203/342 (59.36%), Query Frame = 0


Query: 11  QLVPLDFSSIQTVPKSHVWLDSDEPSSAICLDEKISLPLIDLLDDRASELLGKACEKWGM 70
           +++PLDF++ +T+P SHVW    EP +         +P+I L +     LL +ACE+WG+
Sbjct: 17  RIIPLDFTNTKTLPDSHVW-SKPEPETT-----SGPIPVISLSNPEEHGLLRQACEEWGV 76

Query: 71  FQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVISKHYSKYMWHE 130
           F + +HGV  +L+   + + +RLF+LP   K+  +++PD  TGYG+  IS  Y K MW E
Sbjct: 77  FHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYGVVRISMFYDKLMWSE 136

Query: 131 GFTILGSPI-DDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLDISEEEITNV 190
           GF+++GS +   A  LWP ++  FC VMEEYQ  M  L   L+ +    L ++ E++  +
Sbjct: 137 GFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHEDLGWL 196

Query: 191 LSSIENSGKPN--TALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFN--- 250
           +     SG  +  + L+LNSYP CPDP   MGLA HTD+SL TI++Q    GL+I +   
Sbjct: 197 VPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQE 256

Query: 251 DGVGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSPPADL 310
           +G  W+ + P+ G+L+V +GDL HIISNG+FRS +HR ++  K  HR S AYF  PP +L
Sbjct: 257 EGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVV-NKTHHRVSAAYFAGPPKNL 316

Query: 311 YISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIK 347
            I P     S     PIYR +  +EY   KA +    L++ +
Sbjct: 317 QIGPL---TSDKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348

[Close]

BLAST of Sed0024037 vs. ExPASy Swiss-Prot
Match: Q9FU53 (Gibberellin 3-beta-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA3OX2 PE=1 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.8e-62
Identity = 130/352 (36.93%), Postives = 200/352 (56.82%), Query Frame = 0


Query: 6   LPPPSQL---VPLDFSSIQTVPKSHVW--LDSDEPSSAICLDEKISLPLIDLLDDRASEL 65
           +P PS L   +  DF + + VP++H W  LD            + ++P++D+    A+  
Sbjct: 1   MPTPSHLKNPLCFDFRAARRVPETHAWPGLDDHPVVDGGGGGGEDAVPVVDVGAGDAAAR 60

Query: 66  LGKACEKWGMFQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVIS 125
           + +A E+WG F L+ HGVP  L++  EE   R+F+LP  +KM+ ++ P    GYG   IS
Sbjct: 61  VARAAEQWGAFLLVGHGVPAALLSRVEERVARVFSLPASEKMRAVRGPGEPCGYGSPPIS 120

Query: 126 KHYSKYMWHEGFTILGSPI-DDAKKLWP---SNYKRFCEVMEEYQTKMKGLVEMLLRLTF 185
             +SK MW EG+T   S +  + ++LWP    +Y  FC+VMEE+  +M+ L + LLRL  
Sbjct: 121 SFFSKLMWSEGYTFSPSSLRSELRRLWPKSGDDYLLFCDVMEEFHKEMRRLADELLRLFL 180

Query: 186 KFLDISEEEITNVLSSIENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQAC 245
           + L ++ EE+  V +      +    + LN YP CP+P + +GL AHTD+  FT V Q+ 
Sbjct: 181 RALGLTGEEVAGVEAERRIGERMTATVHLNWYPRCPEPRRALGLIAHTDSGFFTFVLQSL 240

Query: 246 NDGLQIFNDGVG-WVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLA 305
             GLQ+F  G   WV +  ++G  +VNVGDL HI++NGRF S+ HR ++ +  + R SL 
Sbjct: 241 VPGLQLFRRGPDRWVAVPAVAGAFVVNVGDLFHILTNGRFHSVYHRAVVNRDRD-RVSLG 300

Query: 306 YFYSPPADLYISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIKM 348
           YF  PP D  ++P  + +   +  P YR+VT  EY  ++ K  A G S +KM
Sbjct: 301 YFLGPPPDAEVAPLPEAVPAGRS-PAYRAVTWPEYMAVRKKAFATGGSALKM 350

[Close]

BLAST of Sed0024037 vs. ExPASy TrEMBL
Match: Q9LM06 (Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE=2 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 9.2e-150
Identity = 263/349 (75.36%), Postives = 300/349 (85.96%), Query Frame = 0


Query: 1   MADQKLPPPSQLVPLDFSSIQTVPKSHVWLDSDEPSSAICLDEKISLPLIDLLDDRASEL 60
           MADQ+LPPPSQLVPLDF+SIQTVP+SH+W +S E S AI LDE+ISLP+ID LDD ASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  LGKACEKWGMFQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPD-NITGYGMAVI 120
           LG+ACE+WGMFQLINHGVPKTLI E EEET+RLF+LP R KMKTL+      TGYGMA I
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120

Query: 121 SKHYSKYMWHEGFTILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFL 180
           SK++ K MWHEGFT+ GSP+DDAKKLWP++YKRFC+VMEEYQ++MKGL E ++RL FKFL
Sbjct: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180

Query: 181 DISEEEITNVLSSI-ENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACND 240
           DISEEE+  +L+S  E++GKP  ALRLNSYPPCPDP +VMGLAAHTDTSLFTIVHQA +D
Sbjct: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240

Query: 241 GLQIFNDGVGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFY 300
           GLQ+F DG GWVPLSPMSGTLIVNVGD+LHI+SNGRF SILHRVMI +  EHRFSLAYFY
Sbjct: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300

Query: 301 SPPADLYISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIKM 348
            PP DLYISPYCKPLS+S QIP+YR V VKEY TIKAK    GLS IK+
Sbjct: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

[Close]

BLAST of Sed0024037 vs. ExPASy TrEMBL
Match: A0A6J1GIY0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454608 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.4e-145
Identity = 254/347 (73.20%), Postives = 295/347 (85.01%), Query Frame = 0


Query: 1   MADQKLPPPSQLVPLDFSSIQTVPKSHVWLDSDEPSSAICLDEKISLPLIDLLDDRASEL 60
           MADQ      + +PLDF+S+QTVP+SH+W DSDE SS I LDEKISLP IDLLDD ASEL
Sbjct: 1   MADQ------EFLPLDFNSVQTVPESHLWPDSDELSSVIDLDEKISLPSIDLLDDGASEL 60

Query: 61  LGKACEKWGMFQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVIS 120
           LG+ACE+WGMFQL NHG+ KTLIAETEEE +RLFTLP ++KMKTL++ D +TGYG AVIS
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAETEEEARRLFTLPVKEKMKTLRSADRVTGYGTAVIS 120

Query: 121 KHYSKYMWHEGFTILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLD 180
           K++ K MWHEGFTILGSP DDAKKLWPS+ KRFC+VMEEYQ++MK L + L++L FKFLD
Sbjct: 121 KYHPKCMWHEGFTILGSPADDAKKLWPSDSKRFCDVMEEYQSEMKRLSDRLIKLIFKFLD 180

Query: 181 ISEEEITNVLSSIENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGL 240
           IS EE   +LSS+++SGK  TAL+LNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGL
Sbjct: 181 ISVEETMKLLSSMDDSGKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGL 240

Query: 241 QIFNDGVGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSP 300
           QIF D  GWVPLSPM G+ +VNVGDLLHI+SNGRFRSILHRV I+ K  HRFSLAYFY P
Sbjct: 241 QIFKDKAGWVPLSPMRGSFVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYP 300

Query: 301 PADLYISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIKM 348
           P  LYISP+CKPLS+S Q P+YR V+VKEY T+KAKNN +GLS +K+
Sbjct: 301 PPYLYISPHCKPLSESPQTPLYRCVSVKEYFTMKAKNNGEGLSTVKI 341

[Close]

BLAST of Sed0024037 vs. ExPASy TrEMBL
Match: S0BE47 (GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox4 PE=2 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 7.6e-144
Identity = 250/350 (71.43%), Postives = 297/350 (84.86%), Query Frame = 0


Query: 1   MADQKLPPPSQLVPLDFSSIQTVPKSHVWLDSDEPSSAICLDEKISLPLIDLLDDRASEL 60
           MADQ++PPPSQLVPLDF+S+QTVP+SH+W ++ E S+AI LD+++SLPLIDL+ D ASEL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  LGKACEKWGMFQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNI--TGYGMAV 120
           LG+ACE+WGMFQLINHG+ KTLI E EEET+RLFTLP   KMKTL++P ++  TGYGMA 
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 ISKHYSKYMWHEGFTILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKF 180
           ISK++ K MWHEGFTILGSPI++AKKLWP++YKRFC+VMEEYQ +MKGL E ++RL  KF
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180

Query: 181 LDISEEEITNVLSSIENS-GKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACN 240
           L ISEEE+  +L+S + S GKP+ ALRLNSYPPCPDP QVMGLAAHTDTSL TI+HQ  N
Sbjct: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240

Query: 241 DGLQIFNDGVGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYF 300
           +GLQIF DG GWVPLSPMSGTLIVN+GDLLHI+SNGRF SILHRVMI++  EHRFSLAYF
Sbjct: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300

Query: 301 YSPPADLYISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIKM 348
           Y PP ++YISPYCKPLS + Q P+YRSV VKEY  IKAK    GL  IK+
Sbjct: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

[Close]

BLAST of Sed0024037 vs. ExPASy TrEMBL
Match: A0A6J1KKU0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111496619 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.4e-142
Identity = 250/347 (72.05%), Postives = 290/347 (83.57%), Query Frame = 0


Query: 1   MADQKLPPPSQLVPLDFSSIQTVPKSHVWLDSDEPSSAICLDEKISLPLIDLLDDRASEL 60
           MADQ      + +PLDF+S+QTVP+SH+W DS+E SS I LDEKISLP IDLLDD ASEL
Sbjct: 1   MADQ------EFLPLDFNSVQTVPESHLWPDSEELSSVIDLDEKISLPFIDLLDDGASEL 60

Query: 61  LGKACEKWGMFQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVIS 120
           LG+ACE+WGMFQL NHG+ KTLIAE EEE +RLFTLP ++KMK L++ D +TGYG AVIS
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAEMEEEARRLFTLPAKEKMKALRSADRVTGYGTAVIS 120

Query: 121 KHYSKYMWHEGFTILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLD 180
           K++ K MWHEGFTILGSP +DAKKLWPS+ KRFC+VMEEYQ++MKGL + L+RL FKFLD
Sbjct: 121 KYHPKCMWHEGFTILGSPANDAKKLWPSDSKRFCDVMEEYQSQMKGLSDRLIRLIFKFLD 180

Query: 181 ISEEEITNVLSSIENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGL 240
           ISEEE   +LSS  +SGK  TAL+LNSYPPCPDPDQVMGLAAHTDTSLFTIVHQ CNDGL
Sbjct: 181 ISEEETMKLLSSTGDSGKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQVCNDGL 240

Query: 241 QIFNDGVGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSP 300
           QIF D   WVPLSPM G+ +VNVGDLLHI+SNGRF SILHRV I+ K  HRFSLAYFY P
Sbjct: 241 QIFKDRASWVPLSPMRGSFVVNVGDLLHIMSNGRFPSILHRVTIQGKKVHRFSLAYFYYP 300

Query: 301 PADLYISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIKM 348
           P  LYISP+CKPLS+S Q P+YR V+V+EY T+KAKNN +GLS IK+
Sbjct: 301 PPYLYISPHCKPLSESPQAPLYRCVSVEEYFTMKAKNNGEGLSTIKI 341

[Close]

BLAST of Sed0024037 vs. ExPASy TrEMBL
Match: A0A1S3BBH9 (gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488263 PE=3 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.7e-140
Identity = 247/351 (70.37%), Postives = 296/351 (84.33%), Query Frame = 0


Query: 1   MADQKLPPPSQLVPLDFSSIQT-VPKSHVWLDSDEPSSAICLDEKISLPLIDLLDDRASE 60
           MADQ++PPPSQLVPL+F+S+QT VP+SH+W +  E S+A+ LD+++SLPLIDL++D ASE
Sbjct: 1   MADQEIPPPSQLVPLNFASVQTHVPESHLWPNVKELSAAVKLDKRVSLPLIDLVNDDASE 60

Query: 61  LLGKACEKWGMFQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNI--TGYGMA 120
           LLG+ACE+WGMFQLINHGV +TLI E EEET+RLFTLP   KMKTL++P ++  TGYGMA
Sbjct: 61  LLGRACEEWGMFQLINHGVSQTLIEEAEEETRRLFTLPAAQKMKTLRSPGSLTATGYGMA 120

Query: 121 VISKHYSKYMWHEGFTILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFK 180
            ISK++ K MWHEGFTI+GSPI++AKKLWP++YKRFC+VMEEYQ +MKGL E ++RL  K
Sbjct: 121 GISKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKRFCDVMEEYQRQMKGLGERIIRLILK 180

Query: 181 FLDISEEEITNVLSSIENS-GKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQAC 240
           FL ISEEE+  +L+S + S GKP  ALRLNSYPPCPDP QVMGLAAHTDTSL TI+HQ  
Sbjct: 181 FLGISEEEMMKLLTSTDESIGKPYMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVR 240

Query: 241 NDGLQIFNDGVGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAY 300
           ++GLQIF DG GWVPLSPMSGTL+VNVGDLLHI+SNGRF SILHRVMI++  EHRFSLAY
Sbjct: 241 SNGLQIFKDGTGWVPLSPMSGTLVVNVGDLLHILSNGRFTSILHRVMIQENKEHRFSLAY 300

Query: 301 FYSPPADLYISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIKM 348
           FY PP D+YISPYCKPLS + Q P+YRSV VKEY  IKAK    GL  IK+
Sbjct: 301 FYDPPGDIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKMGSGLPAIKI 351

[Close]

BLAST of Sed0024037 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 285.0 bits (728), Expect = 7.9e-77
Identity = 148/334 (44.31%), Postives = 211/334 (63.17%), Query Frame = 0


Query: 14  PLDFSSIQTVPKSHVWLDSDE-PSSAICLDEKISLPLIDLLDDRASELLGKACEKWGMFQ 73
           P DF S+   P S+ W   D+   SA   DE  +LPLIDL D   + L+G AC  WG FQ
Sbjct: 21  PPDFKSL---PDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTTWGAFQ 80

Query: 74  LINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVISKHYSKYMWHEGF 133
           + NHGVP  L+ + E  T  LF LP + K+K  ++ + ++GYG+A I+  ++K MW EGF
Sbjct: 81  ITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIASFFNKKMWSEGF 140

Query: 134 TILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLDISEEEITNVLSS 193
           T++GSP+ D +KLWPS++ ++CE++EEY+  M+ L   L+      L + E++I     +
Sbjct: 141 TVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEEKDIQWAGPN 200

Query: 194 IENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFNDGVGWVPL 253
            +  G     ++LN YP CP+PD+ MGLAAHTD++L TI++Q    GLQ+F D VGWV  
Sbjct: 201 SDFQG-TQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWVTA 260

Query: 254 SPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSPPADLYISPYCKP 313
            P+ G+L+VNVGDLLHI++NG F S+LHR  +      RFS+AY + PP+D+ ISP  K 
Sbjct: 261 PPVPGSLVVNVGDLLHILTNGIFPSVLHRARV-NHVRSRFSMAYLWGPPSDIMISPLPK- 320

Query: 314 LSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIK 347
           L    Q P+Y S+T K+Y   KA +    LSII+
Sbjct: 321 LVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of Sed0024037 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 280.0 bits (715), Expect = 2.5e-75
Identity = 149/345 (43.19%), Postives = 218/345 (63.19%), Query Frame = 0


Query: 9   PSQLVPLDFSSIQTVPKSHVWLDSDE------PSSAICLDEKISLPLIDLLDDRASELLG 68
           P   +P DF+S++ +P S+ W   D+      PS     +   ++PLIDL    A+  +G
Sbjct: 17  PHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGE---NIPLIDLDHPDATNQIG 76

Query: 69  KACEKWGMFQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVISKH 128
            AC  WG FQ+ NHGVP  L+ + E  T  LF LP + K+K+ ++   ++GYG+A I+  
Sbjct: 77  HACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGYGVARIASF 136

Query: 129 YSKYMWHEGFTILGSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLDIS 188
           ++K MW EGFTI GSP++D +KLWP ++  +C+++EEY+  MK L   L+ L    L +S
Sbjct: 137 FNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVS 196

Query: 189 EEEIT-NVLSSIENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQ 248
           EE+I    LSS  N  +   AL+LN YP CP+PD+ MGLAAHTD++L TI++Q    GLQ
Sbjct: 197 EEDIEWASLSSDLNWAQ--AALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQ 256

Query: 249 IFNDGVGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSPP 308
           +F D +GWV + P  G+L+VNVGDL HI+SNG F+S+LHR  +  +   R S+A+ + P 
Sbjct: 257 VFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARV-NQTRARLSVAFLWGPQ 316

Query: 309 ADLYISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIK 347
           +D+ ISP  K +S  +  P+Y+SVT KEY   KA +    LS+I+
Sbjct: 317 SDIKISPVPKLVSPVES-PLYQSVTWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of Sed0024037 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 253.1 bits (645), Expect = 3.3e-67
Identity = 136/342 (39.77%), Postives = 203/342 (59.36%), Query Frame = 0


Query: 11  QLVPLDFSSIQTVPKSHVWLDSDEPSSAICLDEKISLPLIDLLDDRASELLGKACEKWGM 70
           +++PLDF++ +T+P SHVW    EP +         +P+I L +     LL +ACE+WG+
Sbjct: 17  RIIPLDFTNTKTLPDSHVW-SKPEPETT-----SGPIPVISLSNPEEHGLLRQACEEWGV 76

Query: 71  FQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVISKHYSKYMWHE 130
           F + +HGV  +L+   + + +RLF+LP   K+  +++PD  TGYG+  IS  Y K MW E
Sbjct: 77  FHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYGVVRISMFYDKLMWSE 136

Query: 131 GFTILGSPI-DDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLDISEEEITNV 190
           GF+++GS +   A  LWP ++  FC VMEEYQ  M  L   L+ +    L ++ E++  +
Sbjct: 137 GFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHEDLGWL 196

Query: 191 LSSIENSGKPN--TALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFN--- 250
           +     SG  +  + L+LNSYP CPDP   MGLA HTD+SL TI++Q    GL+I +   
Sbjct: 197 VPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQE 256

Query: 251 DGVGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSPPADL 310
           +G  W+ + P+ G+L+V +GDL HIISNG+FRS +HR ++  K  HR S AYF  PP +L
Sbjct: 257 EGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVV-NKTHHRVSAAYFAGPPKNL 316

Query: 311 YISPYCKPLSKSQQIPIYRSVTVKEYTTIKAKNNADGLSIIK 347
            I P     S     PIYR +  +EY   KA +    L++ +
Sbjct: 317 QIGPL---TSDKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348

[Close]

BLAST of Sed0024037 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 234.6 bits (597), Expect = 1.2e-61
Identity = 128/329 (38.91%), Postives = 196/329 (59.57%), Query Frame = 0


Query: 16  DFSSIQTVPKSHVW----LDS-DEPSSAICLDE-KISLPLIDLLDDRASELLGKACEKWG 75
           +  S+QT+P+S  W     DS   PSSA+  D  + S+P+IDL +   + L+G A + WG
Sbjct: 12  NLGSLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWG 71

Query: 76  MFQLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVISKHYSKYMWH 135
            FQ+ NHG+ + L+ + E  ++ LF +P+  K++   +   ++GYG   IS  + K MW 
Sbjct: 72  AFQIANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWS 131

Query: 136 EGFTIL-GSPIDDAKKLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLDISEEEITN 195
           EGFTI   S  +    LWP ++ ++C +++EY  +M+ L   LL      L ++ E+I  
Sbjct: 132 EGFTIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDI-E 191

Query: 196 VLSSIENSGKP--NTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFNDG 255
               +E SG      A+RLN YP CP+P++ MGLAAHTD+++ TI+HQ+   GLQ+F + 
Sbjct: 192 WAHKLEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREE 251

Query: 256 VGWVPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSPPA-DLY 315
            GWV + P  G L+VN+GDL HI+SNG+  S++HR  +      R S+AY +  PA D+ 
Sbjct: 252 SGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKV-NHTRSRISIAYLWGGPAGDVQ 311

Query: 316 ISPYCKPLSKSQQIPIYRSVTVKEYTTIK 335
           I+P  K L+   +  +YRS+T KEY  IK
Sbjct: 312 IAPISK-LTGPAEPSLYRSITWKEYLQIK 337

[Close]

BLAST of Sed0024037 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 193.7 bits (491), Expect = 2.4e-49
Identity = 112/332 (33.73%), Postives = 180/332 (54.22%), Query Frame = 0


Query: 19  SIQTVPKSHVWLDSDEPSSAICLDEKISLPLIDL-------LDDRASELLGKACEKWGMF 78
           +I TVP  +V  D D+       D KI +P+ID+         D   E L  AC++WG F
Sbjct: 25  TITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFF 84

Query: 79  QLINHGVPKTLIAETEEETQRLFTLPTRDKMKTLQAPDNITGYGMAVISKHYSKYMWHEG 138
           QL+NHG+  + + + + E Q  F LP  +K K  Q PD I G+G A +     K  W + 
Sbjct: 85  QLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADL 144

Query: 139 FTILGSPIDDAK-KLWPSNYKRFCEVMEEYQTKMKGLVEMLLRLTFKFLDISEEEITNVL 198
           F     P++  K  L+P     F + +E Y ++++ + ++L+    + L+I  EE+  + 
Sbjct: 145 FFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARALEIKPEELEKLF 204

Query: 199 SSIENSGKPNTALRLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACN-DGLQIFNDGVGW 258
             +++      ++R+N YPPCP PDQV+GL  H+D+   T++ Q  + +GLQI  DG  W
Sbjct: 205 DDVDSV----QSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKKDG-KW 264

Query: 259 VPLSPMSGTLIVNVGDLLHIISNGRFRSILHRVMIRKKAEHRFSLAYFYSPPADLYISPY 318
           VP+ P+    IVN+GD+L II+NG +RSI HR ++  + E R S+A F++      + P 
Sbjct: 265 VPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKE-RLSIATFHNVGMYKEVGP- 324

Query: 319 CKPLSKSQQIPIYRSVTVKEYTTIKAKNNADG 342
            K L + Q++  ++ +T+KEY         DG
Sbjct: 325 AKSLVERQKVARFKRLTMKEYNDGLFSRTLDG 349

The following BLAST results are available for this feature:

BLAST of Sed0024037 vs. NCBI nr


Match Name	E-value	Identity	Description
AAF80661.1	1.9e-149	75.36	putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris]	[more]
XP_038885582.1	4.7e-148	73.41	gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida]	[more]
XP_022951873.1	2.9e-145	73.20	gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata]	[more]
XP_023537854.1	7.0e-144	73.59	gibberellin 3-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo]	[more]
NP_001275523.1	1.6e-143	71.43	gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >KAE8652189.1 hypothetic...	[more]

BLAST of Sed0024037 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24648	7.4e-80	43.45	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1	[more]
Q9ZT84	1.1e-75	44.31	Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 ...	[more]
Q39103	3.6e-74	43.19	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ...	[more]
Q9SVS8	4.7e-66	39.77	Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 ...	[more]
Q9FU53	1.8e-62	36.93	Gibberellin 3-beta-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA3...	[more]

BLAST of Sed0024037 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q9LM06	9.2e-150	75.36	Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE...	[more]
A0A6J1GIY0	1.4e-145	73.20	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454...	[more]
S0BE47	7.6e-144	71.43	GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox4 PE=2 SV=1	[more]
A0A6J1KKU0	1.4e-142	72.05	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11149661...	[more]
A0A1S3BBH9	1.7e-140	70.37	gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488263 PE...	[more]

BLAST of Sed0024037 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80340.1	7.9e-77	44.31	gibberellin 3-oxidase 2	[more]
AT1G15550.1	2.5e-75	43.19	gibberellin 3-oxidase 1	[more]
AT4G21690.1	3.3e-67	39.77	gibberellin 3-oxidase 3	[more]
AT1G80330.1	1.2e-61	38.91	gibberellin 3-oxidase 4	[more]
AT1G17020.1	2.4e-49	33.73	senescence-related gene 1	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 11..332 e-value: 6.1E-79 score: 267.9
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 202..300 e-value: 1.2E-25 score: 89.9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 48..148 e-value: 4.5E-19 score: 69.3
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 11..337
None	No IPR available	PANTHER	PTHR47991:SF77	GIBBERELLIN 3-BETA-DIOXYGENASE 1-LIKE	coord: 11..337
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 13..339
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 198..300 score: 12.65136

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0024037.1	Sed0024037.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding