Sed0024029 (gene) Chayote v1

BLAST of Sed0024029 vs. NCBI nr
Match: KAG7015844.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 861.7 bits (2225), Expect = 3.3e-246
Identity = 436/527 (82.73%), Postives = 469/527 (88.99%), Query Frame = 0

Query: 5   SPANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTD 64
           SPANF PLSPV F KRAA VY  RPSV+YG RVFTWSETY R L+L+SALVHHFHISP D
Sbjct: 7   SPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHFHISPGD 66

Query: 65  LVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPIV 124
           +VAAMAPN+PELYELHFAVPMAGAIISPLNTKLD+PTL+LLLQQL PK+IFLDS FLP V
Sbjct: 67  VVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTV 126

Query: 125 LKSLSQN-GNSIKIPALVLIPADPDTPPSEFLNYNQVLQMTFSDFTPQPIGELDPISVNC 184
           LKSLSQ  GNSI+ PALVLIPA PDTPPSEFL+YN+VL M F DFTP+   ELD IS+NC
Sbjct: 127 LKSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELDAISINC 186

Query: 185 TSGSTGLHKGVVYSHRAAYLNSLATMFRTRIC-NTPSPVFLWTVDMFRCNGWCFVWAMAA 244
           TSGSTGLHKGVVYSHRAAYLNSLAT+FR+ IC NT SPVFLWTVDMFRCNGWCF+WAMAA
Sbjct: 187 TSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAA 246

Query: 245 LGGCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMISESS---NRKP-LSRRVDLVVA 304
           LGGCN+CLR VTADAIF N+ELHKVTLLCGP+TLLKMI ESS   N KP  SRRVDL+VA
Sbjct: 247 LGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRRVDLIVA 306

Query: 305 GALPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITSLEIDVK 364
           GALPI EILTKV+E GF+ISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDL TSLEIDVK
Sbjct: 307 GALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTSLEIDVK 366

Query: 365 DPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDWYRTGDVAVRHKSGRIE 424
           DPMSMESVL DGETLGEVML+GNTLMSGYYKNLKAT +AF GDWYRTGDV VRHKSGRIE
Sbjct: 367 DPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRHKSGRIE 426

Query: 425 VKDRAKDVVVRADG-ATVSTVAVEAVLTSHPKVVEAAVIGFGERLCGFVKLKNGSRVSEE 484
           +KDRAKD+VVRA+G ATVSTV VEAVL SHP V EAAV+  GERLCGFVKLKNGSR S E
Sbjct: 427 MKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVV--GERLCGFVKLKNGSRASAE 486

Query: 485 EIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREKIKALNTS 525
           EIVEFC  HLP+FM+PERVVFGDLPMNS GKVQKF VREK K LNT+
Sbjct: 487 EIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTN 531

BLAST of Sed0024029 vs. NCBI nr
Match: KAG6577804.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 855.9 bits (2210), Expect = 1.8e-244
Identity = 433/527 (82.16%), Postives = 466/527 (88.43%), Query Frame = 0

Query: 5   SPANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTD 64
           SPANF PLSPV F KRAA VY  RPSV+YG RVFTWSETY R L+L+SALVHHFHISP D
Sbjct: 7   SPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHFHISPGD 66

Query: 65  LVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPIV 124
           +VAAMAPN+PELYELHFAVPMAGAIISPLNTKLD+PTL+LLLQQL PK+IFLDS FLP V
Sbjct: 67  VVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTV 126

Query: 125 LKSLSQN-GNSIKIPALVLIPADPDTPPSEFLNYNQVLQMTFSDFTPQPIGELDPISVNC 184
           LKSLSQ  GNSI+ PALVLIP  PDTPPSEFL+YN+VL M F DFTP+   ELD IS+NC
Sbjct: 127 LKSLSQTYGNSIQFPALVLIPTHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELDAISINC 186

Query: 185 TSGSTGLHKGVVYSHRAAYLNSLATMFRTRIC-NTPSPVFLWTVDMFRCNGWCFVWAMAA 244
           TSGSTGLHKGVVYSHRAAYLNSLAT+FR+ IC NT SPVFLWTVDMFRCNGWCF+WAMAA
Sbjct: 187 TSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAA 246

Query: 245 LGGCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMISESS---NRKP-LSRRVDLVVA 304
           LGGCN+CLR VTADAIF N+ELHKVTLLCGP+TLLKMI ESS   N KP  SRRVDL+VA
Sbjct: 247 LGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRRVDLIVA 306

Query: 305 GALPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITSLEIDVK 364
           GALPI EILTKV+E GF+ISYGYGMTEA+GPA+IRPWKPNFDEE VQFEDL TSLEIDVK
Sbjct: 307 GALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEEIVQFEDLFTSLEIDVK 366

Query: 365 DPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDWYRTGDVAVRHKSGRIE 424
           DPMSMESVL DGETLGEVML+GNTLMSGYYKNLKAT +AF GDWYRTGDV VRHKSGRIE
Sbjct: 367 DPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRHKSGRIE 426

Query: 425 VKDRAKDVVVRADG-ATVSTVAVEAVLTSHPKVVEAAVIGFGERLCGFVKLKNGSRVSEE 484
           +KDRAKD+VVRA+G A VSTV VEAVL SHP V EAAV+  GERLCGFVKLKNGSR S E
Sbjct: 427 MKDRAKDIVVRANGEAAVSTVQVEAVLMSHPSVAEAAVV--GERLCGFVKLKNGSRASAE 486

Query: 485 EIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREKIKALNTS 525
           EIVEFC  HLP+FM+PERVVFGDLPMNS GKVQKF VREK K LNT+
Sbjct: 487 EIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTN 531

BLAST of Sed0024029 vs. NCBI nr
Match: XP_022923197.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 855.1 bits (2208), Expect = 3.1e-244
Identity = 430/527 (81.59%), Postives = 469/527 (88.99%), Query Frame = 0

Query: 5   SPANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTD 64
           SPANF PLSPV FLK++A VY  RPSV+YG RVFTWSETY R L+L+SALVHHFH+SP D
Sbjct: 55  SPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHFHVSPGD 114

Query: 65  LVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPIV 124
           +VAAMAPN+PELYELHFAVPMAGAIISPLNTKLD+PTL+LLLQQL PK+IFLDS FLP V
Sbjct: 115 VVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTV 174

Query: 125 LKSLSQN-GNSIKIPALVLIPADPDTPPSEFLNYNQVLQMTFSDFTPQPIGELDPISVNC 184
           L+SLSQ  GNSI+ PALVLIPA PDTPPSEFL+YN+VL M F DFTP+   ELD IS+NC
Sbjct: 175 LQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELDAISINC 234

Query: 185 TSGSTGLHKGVVYSHRAAYLNSLATMFRTRIC-NTPSPVFLWTVDMFRCNGWCFVWAMAA 244
           TSGSTGLHKGVVYSHRAAYLNSLAT+FR+ IC NT SPVFLWTVDMFRCNGWCF+WAMAA
Sbjct: 235 TSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAA 294

Query: 245 LGGCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMISESS---NRKP-LSRRVDLVVA 304
           LGGCN+CLR VT DAIF N+ELHKVTLLCGP+TLLKMI ESS   N KP LSRRVDL+VA
Sbjct: 295 LGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRRVDLIVA 354

Query: 305 GALPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITSLEIDVK 364
           GALPI EILTKV+E GF+ISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDLITSLEIDVK
Sbjct: 355 GALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLITSLEIDVK 414

Query: 365 DPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDWYRTGDVAVRHKSGRIE 424
           DP+SMESVL DGETLGEVML+GNTLMSGYYKNLKAT +AF GDWYRTGDV VRHKSGRIE
Sbjct: 415 DPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRHKSGRIE 474

Query: 425 VKDRAKDVVVRADG-ATVSTVAVEAVLTSHPKVVEAAVIGFGERLCGFVKLKNGSRVSEE 484
           +KDRAKD+VVRA+G   VSTV VEAVL SHP V EAAV+  GERLCGFVKLKNGSR S E
Sbjct: 475 MKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVV--GERLCGFVKLKNGSRASAE 534

Query: 485 EIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREKIKALNTS 525
           EIV+FC  HLP+FM+PERVVFGDLPMNS GKVQKF  REK KALNT+
Sbjct: 535 EIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTN 579

BLAST of Sed0024029 vs. NCBI nr
Match: XP_023552791.1 (probable acyl-activating enzyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852.8 bits (2202), Expect = 1.5e-243
Identity = 429/526 (81.56%), Postives = 469/526 (89.16%), Query Frame = 0

Query: 6   PANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTDL 65
           PANF PLSP+ F  ++A VYG RPS++YG RVFTWSETY RCL+L+SALVHHFHISP D+
Sbjct: 8   PANFVPLSPLTFFNKSAAVYGARPSLIYGARVFTWSETYGRCLHLASALVHHFHISPGDV 67

Query: 66  VAAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPIVL 125
           VAAMAPN+PELYELHFAVPMAGAIISPLNTKLD+PTL+LLLQQL+PK+IFLDS FLP VL
Sbjct: 68  VAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLDPKLIFLDSDFLPTVL 127

Query: 126 KSLSQN-GNSIKIPALVLIPADPDTPPSEFLNYNQVLQMTFSDFTPQPIGELDPISVNCT 185
           KSLSQ  GNS++ PALVLIPA PDTP SEFL+YN+VL M F DFTP+   ELD IS+NCT
Sbjct: 128 KSLSQTYGNSVQFPALVLIPAHPDTPLSEFLDYNRVLGMRFDDFTPRTNAELDAISINCT 187

Query: 186 SGSTGLHKGVVYSHRAAYLNSLATMFRTRIC-NTPSPVFLWTVDMFRCNGWCFVWAMAAL 245
           SGSTGLHKGVVYSHRAAYLNSLAT+FR+ IC NT SPVFLWTVDMFRCNGWCF+WAMAAL
Sbjct: 188 SGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAAL 247

Query: 246 GGCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMISESS---NRKP-LSRRVDLVVAG 305
           GGCN+CLR VT + IF N+ELHKVTLLCGP+TLLKMI ESS   N KP LSRRVDL+VAG
Sbjct: 248 GGCNICLRNVTTNVIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRRVDLIVAG 307

Query: 306 ALPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITSLEIDVKD 365
           ALPI EILTKV+E GF+ISYGYGMTEA+GPA+IRPWKPNF EETVQFEDLITSLEIDVKD
Sbjct: 308 ALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFGEETVQFEDLITSLEIDVKD 367

Query: 366 PMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDWYRTGDVAVRHKSGRIEV 425
           P+SMESVL DGETLGEVML+GNTLMSGYYKNLKAT +AF GDWYRTGDV VRHKSGRIE+
Sbjct: 368 PLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRHKSGRIEM 427

Query: 426 KDRAKDVVVRADG-ATVSTVAVEAVLTSHPKVVEAAVIGFGERLCGFVKLKNGSRVSEEE 485
           KDRAKD+VVRA+G A VSTV VEAVL SHP VVEAAVI  GERLCGFVKLKNGSR S EE
Sbjct: 428 KDRAKDIVVRANGEAAVSTVEVEAVLMSHPSVVEAAVI--GERLCGFVKLKNGSRASAEE 487

Query: 486 IVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREKIKALNTS 525
           IVEFC  HLP+FM+PERVVFGDLPMNS GKVQKF VREK KALNT+
Sbjct: 488 IVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKALNTN 531

BLAST of Sed0024029 vs. NCBI nr
Match: XP_023007650.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima])

HSP 1 Score: 850.9 bits (2197), Expect = 5.8e-243
Identity = 427/527 (81.02%), Postives = 466/527 (88.43%), Query Frame = 0

Query: 5   SPANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTD 64
           SPANF PLSP+ F KRAA VYGGRPSV+YG RVFTWSETY RCL+L+SALVHHFHISP D
Sbjct: 48  SPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHFHISPGD 107

Query: 65  LVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPIV 124
           +VAAMAPN+PELYELHFAVPMAGAIISPLNTKLD+PTL+LLLQQL PK+IFLDS FLP +
Sbjct: 108 VVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDSDFLPTL 167

Query: 125 LKSLSQN-GNSIKIPALVLIPADPDTPPSEFLNYNQVLQMTFSDFTPQPIGELDPISVNC 184
           L+SLSQ  GNSI+ PALVLIPA PDTPPSEFL+YN+VL+M F +FTP+   ELD IS+NC
Sbjct: 168 LQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLEMRFDEFTPRTNSELDAISINC 227

Query: 185 TSGSTGLHKGVVYSHRAAYLNSLATMFRTRIC-NTPSPVFLWTVDMFRCNGWCFVWAMAA 244
           TSGSTGLHKGVVYSHRAAYLNSLAT+FR+ IC NT SPVFLWTVDMFRCNGWCF+WAMAA
Sbjct: 228 TSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAA 287

Query: 245 LGGCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMISESS---NRKP-LSRRVDLVVA 304
           LGGCN+CLR VT DAIF N+ELHKVTLLCGP+TLLKMI ESS   N KP   RRVDL+VA
Sbjct: 288 LGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRRVDLIVA 347

Query: 305 GALPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITSLEIDVK 364
           GALPI EILTKV+E GF+ISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDLI SLEIDVK
Sbjct: 348 GALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLIASLEIDVK 407

Query: 365 DPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDWYRTGDVAVRHKSGRIE 424
           DP SMESVL DGETLGEVML+GN+LMSGYYKNLKAT +AF GDWYRTGDV V+HKSGRIE
Sbjct: 408 DPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKHKSGRIE 467

Query: 425 VKDRAKDVVVRADG-ATVSTVAVEAVLTSHPKVVEAAVIGFGERLCGFVKLKNGSRVSEE 484
           +KDRAKD+VVR +G   VSTV VEAVL SHP V EAAVI  GERLCG VKLKNGSR S E
Sbjct: 468 MKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI--GERLCGLVKLKNGSRASAE 527

Query: 485 EIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREKIKALNTS 525
           EIVEFC  HLP+FM+PERVVFGDLPMNS GKVQKF  REK KALNT+
Sbjct: 528 EIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTN 572

BLAST of Sed0024029 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 3.6e-134
Identity = 261/547 (47.71%), Postives = 359/547 (65.63%), Query Frame = 0

Query: 5   SPANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTD 64
           SPANF+PLSP+ FL+R+A VY  R S+V+G    TW +TY+RCL L+SAL  +  IS  D
Sbjct: 54  SPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NLGISRGD 113

Query: 65  LVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPI- 124
           +VAA+APN+P ++ELHFAVPMAG I+ PLNT+LD  TL++LL   E KI+F+D Q L I 
Sbjct: 114 VVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIA 173

Query: 125 --VLKSLSQNGNSIKIPALVLIPADPDTPPSE-------------FLNYNQVLQMTFSDF 184
              L  L+++  + K   LVLI    D   S+                Y  +L+   S+F
Sbjct: 174 HGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGDSEF 233

Query: 185 -TPQPIGELDPISVNCTSGSTGLHKGVVYSHRAAYLNSLATMFRTRICNTPSPVFLWTVD 244
              +P  E DPIS+N TSG+T   KGVVYSHR AYLNSLAT+F  ++  +  PV+LWTV 
Sbjct: 234 EIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM--SVYPVYLWTVP 293

Query: 245 MFRCNGWCFVWAMAALGGCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMISE--SSN 304
           MF CNGWC VW +AA GG N+CLR V+   IF N+ +HKVT + G  T+L MI     + 
Sbjct: 294 MFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTE 353

Query: 305 RKPLSRRVDLVVAGALPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFDEETVQ 364
            KPL  RV+++  G+ P+P+IL K+ E GF++S+ YG+TE  GP     WKP +D  +++
Sbjct: 354 HKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSLSLE 413

Query: 365 FEDLITSLE---------IDVKDPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCD 424
               + + +         +DVKDP++ME+V  DG T+GEVM +GNT+MSGY+K+++AT  
Sbjct: 414 ERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEATRK 473

Query: 425 AFIGDWYRTGDVAVRHKSGRIEVKDRAKDVVVRADGATVSTVAVEAVLTSHPKVVEAAVI 484
           AF GDW+ +GD+AV++  G IE+KDR KDV++ + G  +S+V VE VL SH  V+EAAV+
Sbjct: 474 AFEGDWFHSGDLAVKYPDGYIEIKDRLKDVII-SGGENISSVEVERVLCSHQAVLEAAVV 533

Query: 485 G-----FGERLCGFVKLKNG-SRVSEEEIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQ 518
                 +G+  CGFVKLK G   +  EEI+ FC  HLP +M P+ +VFGD+P  STGKVQ
Sbjct: 534 ARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGKVQ 593

BLAST of Sed0024029 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 2.0e-129
Identity = 252/544 (46.32%), Postives = 347/544 (63.79%), Query Frame = 0

Query: 5   SPANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTD 64
           SPAN+ PL+P+ FL R+A VY  R S+VYG   +TW +T +RC+ ++SAL     IS  D
Sbjct: 9   SPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQLGISTGD 68

Query: 65  LVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPI- 124
           +V+ +APN+P + ELHF VPMAGA++  LN + DS  +A+LL+    K+IF D QFL I 
Sbjct: 69  VVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIA 128

Query: 125 --VLKSLSQNGNSIKIPALVLIPADPDTP-------PSEFLNYNQVLQMTFSDF-TPQPI 184
               + LS  G+  K+P LVLIP +P T          E + Y  V+ M  SDF   +P 
Sbjct: 129 EGACEILSNKGD--KVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPT 188

Query: 185 GELDPISVNCTSGSTGLHKGVVYSHRAAYLNSLATMFRTRICNTPSPVFLWTVDMFRCNG 244
            E D ISVN TSG+T   KGVVYSHR AYLNSLA +    + +  SP +LWT  MF CNG
Sbjct: 189 DECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHS--SPTYLWTNPMFHCNG 248

Query: 245 WCFVWAMAALGGCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMI--SESSNRKPLSR 304
           WC +W + A+GG N+CLR VTA AIF+N+  HKVT + G  T+L MI  +  S +KPL  
Sbjct: 249 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 308

Query: 305 RVDLVVAGALPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFD----EETVQFE 364
           +V  +   A P   ++ K+ E GF + + YG+TE  GP  I  WKP +D    EE  + +
Sbjct: 309 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 368

Query: 365 -----DLITSLEIDVKDPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDW 424
                + +   EI VKDP++M ++ ADG T+GEV+ +GNT+M+GY KN +AT +AF G W
Sbjct: 369 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 428

Query: 425 YRTGDVAVRHKSGRIEVKDRAKDVVVRADGATVSTVAVEAVLTSHPKVVEAAVIG----- 484
           + +GD+ V+H  G IE+KDR+KD+++ + G  +S++ VE+ L +HP V+EAAV+      
Sbjct: 429 FWSGDLGVKHPDGYIELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVARPDEY 488

Query: 485 FGERLCGFVKLKNGSRVSEEEIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREK 522
           +GE  C FVKLK+GS+ S EE++ +C   LP +M P  +VF DLP  STGKVQKF +R K
Sbjct: 489 WGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTK 545

BLAST of Sed0024029 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 5.5e-127
Identity = 252/538 (46.84%), Postives = 344/538 (63.94%), Query Frame = 0

Query: 7   ANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTDLV 66
           AN  PLSP+ FL+R++  Y    S+VYG   +TW++T+ RCL L+SAL  HF ISP D+V
Sbjct: 37  ANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHFGISPGDVV 96

Query: 67  AAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPIVLK 126
           A  + NIPE+YELHFAVPMAG I+  LN + DS  ++ LL   E K+IF++ Q L     
Sbjct: 97  ATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEPQLLETARA 156

Query: 127 SLSQNG-NSIKIPALVLIPADPDTPPSEFLNYNQVLQMTFSDF-TPQPIGELDPISVNCT 186
           +L       IK P LVL+        S + +YN +L     DF   +P  E DPIS+N T
Sbjct: 157 ALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNECDPISINYT 216

Query: 187 SGSTGLHKGVVYSHRAAYLNSLATMFRTRICNTPSPVFLWTVDMFRCNGWCFVWAMAALG 246
           SG+T   K VVYSHR AYLNS+AT+    +  T S V+LW+V MF CNGWCF W  AA G
Sbjct: 217 SGTTARPKAVVYSHRGAYLNSIATVLLHGM-GTRS-VYLWSVPMFHCNGWCFPWGAAAQG 276

Query: 247 GCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMI---SESSNRKPLSRRVDLVVAGAL 306
             N+C+R V+  AIF+N+ LHKVT      T+L MI    E +   PL  +V+++  G+ 
Sbjct: 277 ATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKVEVMTGGSP 336

Query: 307 PIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFD----EETVQFE-----DLITS 366
           P P+++ ++ E GF +++ YG+TE  GPA     KP +D    EE    +     + +  
Sbjct: 337 PPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPEERYALKARQGLNHLAM 396

Query: 367 LEIDVKDPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDWYRTGDVAVRH 426
            E+DV+DP+SMESV ADG T+GEVM +GNT+MSGY+K+LKAT +AF G W+RTGD+ V+H
Sbjct: 397 EEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFRTGDLGVKH 456

Query: 427 KSGRIEVKDRAKDVVVRADGATVSTVAVEAVLTSHPKVVEAAVIG-----FGERLCGFVK 486
           + G I++KDR KDVV+ + G  VSTV VE VL SH  V+EAAV+      +GE  C FV 
Sbjct: 457 EDGYIQLKDRKKDVVI-SGGENVSTVEVETVLYSHEAVLEAAVVARPDKLWGETPCAFVT 516

Query: 487 LKNG--SRVSEEEIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREKIKALNT 524
           LK G  + VS ++I++FC   LP +M P+ VVF +LP  STGK+QK+ ++EK KA+ +
Sbjct: 517 LKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEKAKAMGS 571

BLAST of Sed0024029 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 5.1e-125
Identity = 245/538 (45.54%), Postives = 341/538 (63.38%), Query Frame = 0

Query: 7   ANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTDLV 66
           AN  PLSP+ FL+R++  Y    S+VYG   +TW++T+ RCL L+SAL  H  ISP D+V
Sbjct: 37  ANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHLGISPGDVV 96

Query: 67  AAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPIVLK 126
           A  + N+PE+YELHFAVPMAG I+  LN + DS  ++ LL   E K+IF++ Q L     
Sbjct: 97  ATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEPQLLETARA 156

Query: 127 SLSQNG-NSIKIPALVLIPADPDTPPSEFLNYNQVLQMTFSDF-TPQPIGELDPISVNCT 186
           +L       IK P LVL+        S + +YN +L     DF   +P  E DPIS+N T
Sbjct: 157 ALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEWDPISINYT 216

Query: 187 SGSTGLHKGVVYSHRAAYLNSLATMFRTRICNTPSPVFLWTVDMFRCNGWCFVWAMAALG 246
           SG+T   K VVYSHR AYLNS+AT+    +  T   V+LW+V MF CNGWCF W  AA G
Sbjct: 217 SGTTARPKAVVYSHRGAYLNSIATVLLHGMGTT--SVYLWSVPMFHCNGWCFPWGAAAQG 276

Query: 247 GCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMI---SESSNRKPLSRRVDLVVAGAL 306
             N+C+R V+  AIF+N+ LHKVT      T+L MI    E +   PL  +V+++  G+ 
Sbjct: 277 ATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKVEVMTGGSP 336

Query: 307 PIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFD----EETVQFE-----DLITS 366
           P P+++ ++ E GF +++ YG+TE  GPA     KP +D    EE    +     + +  
Sbjct: 337 PPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERYALKARQGLNHLAM 396

Query: 367 LEIDVKDPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDWYRTGDVAVRH 426
            E+DV+DP++MESV ADG T+GEVM +GNT+MSGY+K+LKAT +AF G W+R+GD+ V+H
Sbjct: 397 EEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFRSGDLGVKH 456

Query: 427 KSGRIEVKDRAKDVVVRADGATVSTVAVEAVLTSHPKVVEAAVIG-----FGERLCGFVK 486
           + G I++KDR KDVV+ + G  +STV VE VL SH  V+EAAV+      +GE  C FV 
Sbjct: 457 EDGYIQLKDRKKDVVI-SGGENISTVEVETVLYSHEAVLEAAVVARPDKLWGETPCAFVT 516

Query: 487 LKNG--SRVSEEEIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREKIKALNT 524
           LK G  + VS ++I++FC   LP +M P+ VVF +LP  STGK+QK+ ++EK  A+ +
Sbjct: 517 LKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEKAMAMGS 571

BLAST of Sed0024029 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 2.1e-110
Identity = 222/546 (40.66%), Postives = 345/546 (63.19%), Query Frame = 0

Query: 7   ANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTDLV 66
           AN  PL+P+ FLKRA+  Y  R S++YG   FTW +TY+RC  L+++L+   +I+  D+V
Sbjct: 9   ANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SLNITRNDVV 68

Query: 67  AAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPIV-- 126
           + +APN+P +YE+HF+VPM GA+++P+NT+LD+ T+A++L+  EPKI+F+D +F P++  
Sbjct: 69  SILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYEFAPLIQE 128

Query: 127 LKSLSQNGNSIKIPALVLI-PADPDTPP-SEFLNYNQVLQMTFSDFTPQPI-------GE 186
           +  L     S   P ++LI   D  T P S+ L+Y  +++    + TP           E
Sbjct: 129 VLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRK--GEPTPSSSASMFRVHNE 188

Query: 187 LDPISVNCTSGSTGLHKGVVYSHRAAYLNSLATMFRTRICNTPSPVFLWTVDMFRCNGWC 246
            DPIS+N TSG+T   KGVV SH+ AYL++L+++    +     PV+LWT+ MF CNGW 
Sbjct: 189 HDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEM--GIFPVYLWTLPMFHCNGWT 248

Query: 247 FVWAMAALGGCNVCLRTVTADAIFNNVELHKVT-LLCGPTTLLKMISES-SNRKPLSRRV 306
             W++AA GG NVC+R VTA  I+ N+ELH VT + C PT    ++  S +++ P S  V
Sbjct: 249 HTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKSSPV 308

Query: 307 DLVVAGALPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFDE--ETVQFE---- 366
            ++  G+ P   ++ KV + GF + +GYG+TEA GP +   W+  +++  E  Q E    
Sbjct: 309 QVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIELQQR 368

Query: 367 ---DLITSLEIDVKDPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDWYR 426
                +T  ++DVK+  ++ESV  DG+T+GE+++KG++LM GY KN KAT +AF   W  
Sbjct: 369 QGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHGWLN 428

Query: 427 TGDVAVRHKSGRIEVKDRAKDVVVRADGATVSTVAVEAVLTSHPKVVEAAVIG-----FG 486
           TGD+ V H  G +E+KDR+KD+++ + G  +S++ VE VL  + +V+EAAV+      +G
Sbjct: 429 TGDIGVIHPDGYVEIKDRSKDIII-SGGENISSIEVEKVLYMYQEVLEAAVVAMPHPLWG 488

Query: 487 ERLCGFVKLKNGSR---VSEEEIVEFCEFHLPKFMVPERVV-FGDLPMNSTGKVQKFTVR 522
           E  C FV LK G      SE +++++C  ++P FM P++VV F +LP NS GK+ K  +R
Sbjct: 489 ETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSKLR 548

BLAST of Sed0024029 vs. ExPASy TrEMBL
Match: A0A6J1E5I3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430963 PE=4 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.5e-244
Identity = 430/527 (81.59%), Postives = 469/527 (88.99%), Query Frame = 0

Query: 5   SPANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTD 64
           SPANF PLSPV FLK++A VY  RPSV+YG RVFTWSETY R L+L+SALVHHFH+SP D
Sbjct: 55  SPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHFHVSPGD 114

Query: 65  LVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPIV 124
           +VAAMAPN+PELYELHFAVPMAGAIISPLNTKLD+PTL+LLLQQL PK+IFLDS FLP V
Sbjct: 115 VVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTV 174

Query: 125 LKSLSQN-GNSIKIPALVLIPADPDTPPSEFLNYNQVLQMTFSDFTPQPIGELDPISVNC 184
           L+SLSQ  GNSI+ PALVLIPA PDTPPSEFL+YN+VL M F DFTP+   ELD IS+NC
Sbjct: 175 LQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELDAISINC 234

Query: 185 TSGSTGLHKGVVYSHRAAYLNSLATMFRTRIC-NTPSPVFLWTVDMFRCNGWCFVWAMAA 244
           TSGSTGLHKGVVYSHRAAYLNSLAT+FR+ IC NT SPVFLWTVDMFRCNGWCF+WAMAA
Sbjct: 235 TSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAA 294

Query: 245 LGGCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMISESS---NRKP-LSRRVDLVVA 304
           LGGCN+CLR VT DAIF N+ELHKVTLLCGP+TLLKMI ESS   N KP LSRRVDL+VA
Sbjct: 295 LGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRRVDLIVA 354

Query: 305 GALPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITSLEIDVK 364
           GALPI EILTKV+E GF+ISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDLITSLEIDVK
Sbjct: 355 GALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLITSLEIDVK 414

Query: 365 DPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDWYRTGDVAVRHKSGRIE 424
           DP+SMESVL DGETLGEVML+GNTLMSGYYKNLKAT +AF GDWYRTGDV VRHKSGRIE
Sbjct: 415 DPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRHKSGRIE 474

Query: 425 VKDRAKDVVVRADG-ATVSTVAVEAVLTSHPKVVEAAVIGFGERLCGFVKLKNGSRVSEE 484
           +KDRAKD+VVRA+G   VSTV VEAVL SHP V EAAV+  GERLCGFVKLKNGSR S E
Sbjct: 475 MKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVV--GERLCGFVKLKNGSRASAE 534

Query: 485 EIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREKIKALNTS 525
           EIV+FC  HLP+FM+PERVVFGDLPMNS GKVQKF  REK KALNT+
Sbjct: 535 EIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTN 579

BLAST of Sed0024029 vs. ExPASy TrEMBL
Match: A0A6J1L3K3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111500216 PE=4 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 2.8e-243
Identity = 427/527 (81.02%), Postives = 466/527 (88.43%), Query Frame = 0

Query: 5   SPANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTD 64
           SPANF PLSP+ F KRAA VYGGRPSV+YG RVFTWSETY RCL+L+SALVHHFHISP D
Sbjct: 48  SPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHFHISPGD 107

Query: 65  LVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPIV 124
           +VAAMAPN+PELYELHFAVPMAGAIISPLNTKLD+PTL+LLLQQL PK+IFLDS FLP +
Sbjct: 108 VVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDSDFLPTL 167

Query: 125 LKSLSQN-GNSIKIPALVLIPADPDTPPSEFLNYNQVLQMTFSDFTPQPIGELDPISVNC 184
           L+SLSQ  GNSI+ PALVLIPA PDTPPSEFL+YN+VL+M F +FTP+   ELD IS+NC
Sbjct: 168 LQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLEMRFDEFTPRTNSELDAISINC 227

Query: 185 TSGSTGLHKGVVYSHRAAYLNSLATMFRTRIC-NTPSPVFLWTVDMFRCNGWCFVWAMAA 244
           TSGSTGLHKGVVYSHRAAYLNSLAT+FR+ IC NT SPVFLWTVDMFRCNGWCF+WAMAA
Sbjct: 228 TSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAA 287

Query: 245 LGGCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMISESS---NRKP-LSRRVDLVVA 304
           LGGCN+CLR VT DAIF N+ELHKVTLLCGP+TLLKMI ESS   N KP   RRVDL+VA
Sbjct: 288 LGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRRVDLIVA 347

Query: 305 GALPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITSLEIDVK 364
           GALPI EILTKV+E GF+ISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDLI SLEIDVK
Sbjct: 348 GALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLIASLEIDVK 407

Query: 365 DPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDWYRTGDVAVRHKSGRIE 424
           DP SMESVL DGETLGEVML+GN+LMSGYYKNLKAT +AF GDWYRTGDV V+HKSGRIE
Sbjct: 408 DPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKHKSGRIE 467

Query: 425 VKDRAKDVVVRADG-ATVSTVAVEAVLTSHPKVVEAAVIGFGERLCGFVKLKNGSRVSEE 484
           +KDRAKD+VVR +G   VSTV VEAVL SHP V EAAVI  GERLCG VKLKNGSR S E
Sbjct: 468 MKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI--GERLCGLVKLKNGSRASAE 527

Query: 485 EIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREKIKALNTS 525
           EIVEFC  HLP+FM+PERVVFGDLPMNS GKVQKF  REK KALNT+
Sbjct: 528 EIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTN 572

BLAST of Sed0024029 vs. ExPASy TrEMBL
Match: A0A1S3BKP8 (probable acyl-activating enzyme 2 OS=Cucumis melo OX=3656 GN=LOC103490658 PE=4 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 1.1e-226
Identity = 404/531 (76.08%), Postives = 460/531 (86.63%), Query Frame = 0

Query: 3   HFSPANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISP 62
           H  P NFAPLSPV FLKRA+T+YG RPS++YG RVFTWS+TY RCL+++SALVHHFH+SP
Sbjct: 5   HHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHFHLSP 64

Query: 63  TDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLP 122
            DLV AMAPNIPELYELHFAVPMAG IIS LNTKLDSPTL+LLLQQL PK+IFLDSQFLP
Sbjct: 65  ADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLP 124

Query: 123 IVLKSLSQNGNSIKIPALVLIPADPDTP-PSEFLNYNQVLQMTF--SDFTPQPIGELDPI 182
           I+LKSL     SIK PAL+LIP+DP+T  PS+FL+YN++L M     +FTP+P  ELDPI
Sbjct: 125 ILLKSL--ENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPI 184

Query: 183 SVNCTSGSTGLHKGVVYSHRAAYLNSLATMFRTRIC-NTPSPVFLWTVDMFRCNGWCFVW 242
           S+N TSGSTGLHKGV+YSHRAAYLNSLAT+FR++IC +T SPVFLWTVDMFRCNGWCF+W
Sbjct: 185 SINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIW 244

Query: 243 AMAALGGCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMISESSNR----KPLSRRVD 302
            MAALGGCN+CLRTVTADAIF NVELH+VTLLCGP+TLLKMI ESS+     + LSRRVD
Sbjct: 245 VMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVD 304

Query: 303 LVVAGALPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNF-DEETVQFED-LITS 362
           L+VAGALPI EILTKV+E GF+ISYGYGMTEA+GPAIIRPWKP+F D++ VQF+D LITS
Sbjct: 305 LIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITS 364

Query: 363 LEIDVKDPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGD-WYRTGDVAVR 422
           LEIDVKDP+SMESVL DGETLGEVML+GNTLMSGYYKNLKAT +AF+G+ WYRTGDV VR
Sbjct: 365 LEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVR 424

Query: 423 HKSGRIEVKDRAKDVVVRADG-ATVSTVAVEAVLTSHPKVVEAAVIGFGERLCGFVKLKN 482
           HKSGRIE+KDRAKD+VVR DG   VSTV VE VL SHP V E AV+G    L GFVKLKN
Sbjct: 425 HKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVG-ERTLYGFVKLKN 484

Query: 483 GSRVSEEEIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREKIKAL 522
           GS+ + +EIVEFC  HLP+FMVP+ +VFGDLPMNSTGKVQKF +REK KAL
Sbjct: 485 GSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKAL 532

BLAST of Sed0024029 vs. ExPASy TrEMBL
Match: A0A0A0L3L0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002400 PE=4 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 3.2e-202
Identity = 364/463 (78.62%), Postives = 407/463 (87.90%), Query Frame = 0

Query: 69  MAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPIVLKSL 128
           MAPNIPELYELHFAVPMAGAIIS LNTKLDSPTL+LLLQQL PK+IF+DSQFLPI+LKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFIDSQFLPILLKSL 60

Query: 129 SQNGNSIKIPALVLIPADPDTP-PSEFLNYNQVLQMTF--SDFTPQPIGELDPISVNCTS 188
               +SIK PALVLIP+DPDTP PSEFL+YN+VL M F   DFTP+P  ELDPIS+N TS
Sbjct: 61  --ENSSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINYTS 120

Query: 189 GSTGLHKGVVYSHRAAYLNSLATMFRTRIC-NTPSPVFLWTVDMFRCNGWCFVWAMAALG 248
           GSTGLHKGV+YSHRAAYLNSLAT+FR++IC +T SPVFLWTVDMFRCNGWCF+W MAALG
Sbjct: 121 GSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALG 180

Query: 249 GCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMISESSNR----KPLSRRVDLVVAGA 308
           GCN+CLRTVTADAIF NVELH+VTLLCGP TLLKMI ESS+     + LSRRVDL+VAGA
Sbjct: 181 GCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIYESSSNNCMPRRLSRRVDLIVAGA 240

Query: 309 LPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITSLEIDVKDP 368
           LPI EILTKV+E GF+ISYGYGMTEA+GPAIIRPWKP F+E+ VQFEDLITSLEIDVKDP
Sbjct: 241 LPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITSLEIDVKDP 300

Query: 369 MSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGD-WYRTGDVAVRHKSGRIEV 428
           +SMESVL DGETLGEVML+GNTLMSGYYKN+KAT +AFIG+ WYRTGDV VRHKSGRIE+
Sbjct: 301 ISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVRHKSGRIEM 360

Query: 429 KDRAKDVVVRADG-ATVSTVAVEAVLTSHPKVVEAAVIGFGERLCGFVKLKNGSRVSEEE 488
           KDRAKD+VVR DG   VSTV VE VL SHP V EAAV+G    L GFVKLKN S+ + +E
Sbjct: 361 KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAEAAVVG-ERTLYGFVKLKNRSKENGDE 420

Query: 489 IVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREKIKAL 522
           IVEFC  HLP+FM+P+R+VFGDLPMNSTGKVQKF +REK+KAL
Sbjct: 421 IVEFCRMHLPEFMIPKRIVFGDLPMNSTGKVQKFALREKVKAL 460

BLAST of Sed0024029 vs. ExPASy TrEMBL
Match: A0A5A7UEI8 (Putative acyl-activating enzyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00360 PE=4 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 2.9e-195
Identity = 356/465 (76.56%), Postives = 403/465 (86.67%), Query Frame = 0

Query: 69  MAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPIVLKSL 128
           MAPNIPELYELHFAVPMAG IIS LNTKLDSPTL+LLLQQL PK+IFLDSQFLPI+LKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 60

Query: 129 SQNGNSIKIPALVLIPADPDTP-PSEFLNYNQVLQMTF--SDFTPQPIGELDPISVNCTS 188
                SIK PAL+LIP+DP+T  PS+FL+YN++L M     +FTP+P  ELDPIS+N TS
Sbjct: 61  --ENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYTS 120

Query: 189 GSTGLHKGVVYSHRAAYLNSLATMFRTRIC-NTPSPVFLWTVDMFRCNGWCFVWAMAALG 248
           GSTGLHKGV+YSHRAAYLNSLAT+FR++IC +T SPVFLWTVDMFRCNGWCF+W MAALG
Sbjct: 121 GSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALG 180

Query: 249 GCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMISESSNR----KPLSRRVDLVVAGA 308
           GCN+CLRTVTADAIF NVELH+VTLLCGP+TLLKMI ESS+     + LSRRVDL+VAGA
Sbjct: 181 GCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAGA 240

Query: 309 LPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNF-DEETVQFED-LITSLEIDVK 368
           LPI EILTKV+E GF+ISYGYGMTEA+GPAIIRPWKP+F D++ VQF+D LITSLEIDVK
Sbjct: 241 LPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDVK 300

Query: 369 DPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGD-WYRTGDVAVRHKSGRI 428
           DP+SMESVL DGETLGEVML+GNTLMSGYYKNLKAT +AF+G+ WYRTGDV VRHKSGRI
Sbjct: 301 DPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGRI 360

Query: 429 EVKDRAKDVVVRADG-ATVSTVAVEAVLTSHPKVVEAAVIGFGERLCGFVKLKNGSRVSE 488
           E+KDRAKD+VVR DG   VSTV VE VL SHP V E AV+G    L GFVKLKNGS+ + 
Sbjct: 361 EMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVG-ERTLYGFVKLKNGSKENN 420

Query: 489 EEIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREKIKAL 522
           +EIVEFC  HLP+FMVP+ +VFGDLPMNSTGKVQKF +REK KAL
Sbjct: 421 DEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKAL 462

BLAST of Sed0024029 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 479.9 bits (1234), Expect = 2.5e-135
Identity = 261/547 (47.71%), Postives = 359/547 (65.63%), Query Frame = 0

Query: 5   SPANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTD 64
           SPANF+PLSP+ FL+R+A VY  R S+V+G    TW +TY+RCL L+SAL  +  IS  D
Sbjct: 54  SPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NLGISRGD 113

Query: 65  LVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPI- 124
           +VAA+APN+P ++ELHFAVPMAG I+ PLNT+LD  TL++LL   E KI+F+D Q L I 
Sbjct: 114 VVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIA 173

Query: 125 --VLKSLSQNGNSIKIPALVLIPADPDTPPSE-------------FLNYNQVLQMTFSDF 184
              L  L+++  + K   LVLI    D   S+                Y  +L+   S+F
Sbjct: 174 HGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGDSEF 233

Query: 185 -TPQPIGELDPISVNCTSGSTGLHKGVVYSHRAAYLNSLATMFRTRICNTPSPVFLWTVD 244
              +P  E DPIS+N TSG+T   KGVVYSHR AYLNSLAT+F  ++  +  PV+LWTV 
Sbjct: 234 EIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM--SVYPVYLWTVP 293

Query: 245 MFRCNGWCFVWAMAALGGCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMISE--SSN 304
           MF CNGWC VW +AA GG N+CLR V+   IF N+ +HKVT + G  T+L MI     + 
Sbjct: 294 MFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTE 353

Query: 305 RKPLSRRVDLVVAGALPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFDEETVQ 364
            KPL  RV+++  G+ P+P+IL K+ E GF++S+ YG+TE  GP     WKP +D  +++
Sbjct: 354 HKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSLSLE 413

Query: 365 FEDLITSLE---------IDVKDPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCD 424
               + + +         +DVKDP++ME+V  DG T+GEVM +GNT+MSGY+K+++AT  
Sbjct: 414 ERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEATRK 473

Query: 425 AFIGDWYRTGDVAVRHKSGRIEVKDRAKDVVVRADGATVSTVAVEAVLTSHPKVVEAAVI 484
           AF GDW+ +GD+AV++  G IE+KDR KDV++ + G  +S+V VE VL SH  V+EAAV+
Sbjct: 474 AFEGDWFHSGDLAVKYPDGYIEIKDRLKDVII-SGGENISSVEVERVLCSHQAVLEAAVV 533

Query: 485 G-----FGERLCGFVKLKNG-SRVSEEEIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQ 518
                 +G+  CGFVKLK G   +  EEI+ FC  HLP +M P+ +VFGD+P  STGKVQ
Sbjct: 534 ARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGKVQ 593

BLAST of Sed0024029 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 464.2 bits (1193), Expect = 1.4e-130
Identity = 252/544 (46.32%), Postives = 347/544 (63.79%), Query Frame = 0

Query: 5   SPANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTD 64
           SPAN+ PL+P+ FL R+A VY  R S+VYG   +TW +T +RC+ ++SAL     IS  D
Sbjct: 9   SPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQLGISTGD 68

Query: 65  LVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPI- 124
           +V+ +APN+P + ELHF VPMAGA++  LN + DS  +A+LL+    K+IF D QFL I 
Sbjct: 69  VVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIA 128

Query: 125 --VLKSLSQNGNSIKIPALVLIPADPDTP-------PSEFLNYNQVLQMTFSDF-TPQPI 184
               + LS  G+  K+P LVLIP +P T          E + Y  V+ M  SDF   +P 
Sbjct: 129 EGACEILSNKGD--KVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPT 188

Query: 185 GELDPISVNCTSGSTGLHKGVVYSHRAAYLNSLATMFRTRICNTPSPVFLWTVDMFRCNG 244
            E D ISVN TSG+T   KGVVYSHR AYLNSLA +    + +  SP +LWT  MF CNG
Sbjct: 189 DECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHS--SPTYLWTNPMFHCNG 248

Query: 245 WCFVWAMAALGGCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMI--SESSNRKPLSR 304
           WC +W + A+GG N+CLR VTA AIF+N+  HKVT + G  T+L MI  +  S +KPL  
Sbjct: 249 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 308

Query: 305 RVDLVVAGALPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFD----EETVQFE 364
           +V  +   A P   ++ K+ E GF + + YG+TE  GP  I  WKP +D    EE  + +
Sbjct: 309 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 368

Query: 365 -----DLITSLEIDVKDPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDW 424
                + +   EI VKDP++M ++ ADG T+GEV+ +GNT+M+GY KN +AT +AF G W
Sbjct: 369 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 428

Query: 425 YRTGDVAVRHKSGRIEVKDRAKDVVVRADGATVSTVAVEAVLTSHPKVVEAAVIG----- 484
           + +GD+ V+H  G IE+KDR+KD+++ + G  +S++ VE+ L +HP V+EAAV+      
Sbjct: 429 FWSGDLGVKHPDGYIELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVARPDEY 488

Query: 485 FGERLCGFVKLKNGSRVSEEEIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREK 522
           +GE  C FVKLK+GS+ S EE++ +C   LP +M P  +VF DLP  STGKVQKF +R K
Sbjct: 489 WGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTK 545

BLAST of Sed0024029 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 401.0 bits (1029), Expect = 1.5e-111
Identity = 222/546 (40.66%), Postives = 345/546 (63.19%), Query Frame = 0

Query: 7   ANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTDLV 66
           AN  PL+P+ FLKRA+  Y  R S++YG   FTW +TY+RC  L+++L+   +I+  D+V
Sbjct: 9   ANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SLNITRNDVV 68

Query: 67  AAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPIV-- 126
           + +APN+P +YE+HF+VPM GA+++P+NT+LD+ T+A++L+  EPKI+F+D +F P++  
Sbjct: 69  SILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYEFAPLIQE 128

Query: 127 LKSLSQNGNSIKIPALVLI-PADPDTPP-SEFLNYNQVLQMTFSDFTPQPI-------GE 186
           +  L     S   P ++LI   D  T P S+ L+Y  +++    + TP           E
Sbjct: 129 VLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRK--GEPTPSSSASMFRVHNE 188

Query: 187 LDPISVNCTSGSTGLHKGVVYSHRAAYLNSLATMFRTRICNTPSPVFLWTVDMFRCNGWC 246
            DPIS+N TSG+T   KGVV SH+ AYL++L+++    +     PV+LWT+ MF CNGW 
Sbjct: 189 HDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEM--GIFPVYLWTLPMFHCNGWT 248

Query: 247 FVWAMAALGGCNVCLRTVTADAIFNNVELHKVT-LLCGPTTLLKMISES-SNRKPLSRRV 306
             W++AA GG NVC+R VTA  I+ N+ELH VT + C PT    ++  S +++ P S  V
Sbjct: 249 HTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKSSPV 308

Query: 307 DLVVAGALPIPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFDE--ETVQFE---- 366
            ++  G+ P   ++ KV + GF + +GYG+TEA GP +   W+  +++  E  Q E    
Sbjct: 309 QVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIELQQR 368

Query: 367 ---DLITSLEIDVKDPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDWYR 426
                +T  ++DVK+  ++ESV  DG+T+GE+++KG++LM GY KN KAT +AF   W  
Sbjct: 369 QGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHGWLN 428

Query: 427 TGDVAVRHKSGRIEVKDRAKDVVVRADGATVSTVAVEAVLTSHPKVVEAAVIG-----FG 486
           TGD+ V H  G +E+KDR+KD+++ + G  +S++ VE VL  + +V+EAAV+      +G
Sbjct: 429 TGDIGVIHPDGYVEIKDRSKDIII-SGGENISSIEVEKVLYMYQEVLEAAVVAMPHPLWG 488

Query: 487 ERLCGFVKLKNGSR---VSEEEIVEFCEFHLPKFMVPERVV-FGDLPMNSTGKVQKFTVR 522
           E  C FV LK G      SE +++++C  ++P FM P++VV F +LP NS GK+ K  +R
Sbjct: 489 ETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSKLR 548

BLAST of Sed0024029 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 393.7 bits (1010), Expect = 2.4e-109
Identity = 218/473 (46.09%), Postives = 298/473 (63.00%), Query Frame = 0

Query: 76  LYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPI---VLKSLSQNG 135
           + ELHF VPMAGA++  LN + DS  +A+LL+    K+IF D QFL I     + LS  G
Sbjct: 1   MVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKG 60

Query: 136 NSIKIPALVLIPADPDTP-------PSEFLNYNQVLQMTFSDF-TPQPIGELDPISVNCT 195
           +  K+P LVLIP +P T          E + Y  V+ M  SDF   +P  E D ISVN T
Sbjct: 61  D--KVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYT 120

Query: 196 SGSTGLHKGVVYSHRAAYLNSLATMFRTRICNTPSPVFLWTVDMFRCNGWCFVWAMAALG 255
           SG+T   KGVVYSHR AYLNSLA +    + +  SP +LWT  MF CNGWC +W + A+G
Sbjct: 121 SGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHS--SPTYLWTNPMFHCNGWCLLWGVTAIG 180

Query: 256 GCNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMI--SESSNRKPLSRRVDLVVAGALP 315
           G N+CLR VTA AIF+N+  HKVT + G  T+L MI  +  S +KPL  +V  +   A P
Sbjct: 181 GTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGAAPP 240

Query: 316 IPEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNFD----EETVQFE-----DLITSL 375
              ++ K+ E GF + + YG+TE  GP  I  WKP +D    EE  + +     + +   
Sbjct: 241 PAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLGLE 300

Query: 376 EIDVKDPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDWYRTGDVAVRHK 435
           EI VKDP++M ++ ADG T+GEV+ +GNT+M+GY KN +AT +AF G W+ +GD+ V+H 
Sbjct: 301 EIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVKHP 360

Query: 436 SGRIEVKDRAKDVVVRADGATVSTVAVEAVLTSHPKVVEAAVIG-----FGERLCGFVKL 495
            G IE+KDR+KD+++ + G  +S++ VE+ L +HP V+EAAV+      +GE  C FVKL
Sbjct: 361 DGYIELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKL 420

Query: 496 KNGSRVSEEEIVEFCEFHLPKFMVPERVVFGDLPMNSTGKVQKFTVREKIKAL 522
           K+GS+ S EE++ +C   LP +M P  +VF DLP  STGKVQKF +R K KAL
Sbjct: 421 KDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKAL 467

BLAST of Sed0024029 vs. TAIR 10
Match: AT1G76290.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 392.9 bits (1008), Expect = 4.1e-109
Identity = 220/533 (41.28%), Postives = 322/533 (60.41%), Query Frame = 0

Query: 7   ANFAPLSPVGFLKRAATVYGGRPSVVYGGRVFTWSETYERCLNLSSALVHHFHISPTDLV 66
           AN+ PLSP+ FL+RAA V+G R SVVYG   +TW +T +RC+ L+SAL     +S  D+V
Sbjct: 9   ANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASAL-SDLGLSRHDVV 68

Query: 67  AAMAPNIPELYELHFAVPMAGAIISPLNTKLDSPTLALLLQQLEPKIIFLDSQFLPIVLK 126
           AA+APN+P L EL+F  PMAGA++  LNT  DS  LA+ L++ +PK+ F+DS+FL +  +
Sbjct: 69  AALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMALEKTKPKVFFVDSEFLSVAEE 128

Query: 127 SLSQNGNSIKIPALVLIPADPDTPPSEFLNYNQVLQMTFSDFTP-QPIGELDPISVNCTS 186
           SLS   N  + P ++ I  +P T  S++  Y   L     +F P +P+ E DPI++N TS
Sbjct: 129 SLSLLSNIEEKPLIITITENP-TEQSKYEQYEDFLSTGNPNFKPIRPVDECDPIALNFTS 188

Query: 187 GSTGLHKGVVYSHRAAYLNSLATMFRTRICNTPSPVFLWTVDMFRCNGWCFVWAMAALGG 246
           G+T   K VVYSHR AYLN+ A      +   P PV+L TV M+ C+GWC++W + A GG
Sbjct: 189 GTTSTPKCVVYSHRGAYLNATAVGVMNEM--KPMPVYLCTVPMYHCSGWCYIWTVTAFGG 248

Query: 247 CNVCLRTVTADAIFNNVELHKVTLLCGPTTLLKMISES--SNRKPLSRRVDLVVAGALPI 306
             VCLR V  + IF+++  HKVT   G   +L MI+ +  S +K     V ++  G+ P 
Sbjct: 249 VIVCLREVNDEVIFDSIVKHKVTNFGGSPPVLNMIANARDSVKKSFPWTVQVMSGGSSP- 308

Query: 307 PEILTKVSEFGFDISYGYGMTEALGPAIIRPWKPNF----DEETVQFE-----DLITSLE 366
           PE++ K+ + GF +   YG +E  G      W P +    +EE+++ +     +      
Sbjct: 309 PEVMLKLKKLGFKVMMAYGCSEVYGLGTACLWMPEWETLPEEESLRLKARDGLNHFAKEA 368

Query: 367 IDVKDPMSMESVLADGETLGEVMLKGNTLMSGYYKNLKATCDAFIGDWYRTGDVAVRHKS 426
           +DV DP +M+SV  DG+T+  + L+GNT+MSGY+K+ +AT  AF G WY + D+ V    
Sbjct: 369 VDVLDPTTMKSVPHDGKTIRVIALRGNTVMSGYFKDKEATEAAFRGGWYWSRDMGVIDPD 428

Query: 427 GRIEVKDRAKDVVVRADGATVSTVAVEAVLTSHPKVVEAAVIG-----FGERLCGFVKLK 486
           G I+ KDR++DV+    G  V +  +E +L SHP V +A V+G      GE +C FVKLK
Sbjct: 429 GYIQFKDRSQDVIT-CGGEIVGSKEIEGILYSHPAVYDAGVVGRPDETLGESMCAFVKLK 488

Query: 487 NGSRVSEEEIVEFCEFHLPK---FMVPERVVFGDLPMNSTGKVQKFTVREKIK 520
            G+   EEEI+EFC+  L      M+P+ VVF D+P   TGK++K  +R+  K
Sbjct: 489 EGAEAREEEIIEFCKRKLGNKNMKMIPKTVVFSDVPKTPTGKIRKNVLRKMAK 535