Sed0023995 (gene) Chayote v1

Overview
NameSed0023995
Typegene
OrganismSechium edule (Chayote v1)
Description(1->3)-beta-glucan endohydrolase
LocationLG13: 19337098 .. 19340356 (-)
RNA-Seq ExpressionSed0023995
SyntenySed0023995
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGCAAATGTTACCTCGCAAGAATATTCTAAAAGGATTTAAAATAAAAAATGATTAACCTTCAAGAGCAATATATAGCTTATTTGTATATTTTCCAGCGAACCATGGGCCTGTTAATGGGCCTAAAAGAATCCCGAGATGCGTGGGCCCCACCTTCTGCACCACTGCCCCACTCTCTACTTCGAATTTTCACTTTTCAACTTTTCCAGTTTTCCCTCTCCTCAGCGCCAATGGCTTGCTCTCCGTTTCTCTCTCTCCTGCTCTTCTCTCTGCTCCTCCGCCTCTCCGCCGCCGCCTACTCCATCGGCGTCAACTACGGCACCGTCGCCGACAACCTCCCTCCGCCCTCCCAGGTCGCCGCCTTCCTCAAATCCCACACCACCATCGACCGCGTCAAGATCTTCGACGCCAACCCCGACATCCTGCACGCCTTCGCCGGCACCGCCATCTCCCTCACCGTCTCCGTCGCCAACGCCGACATCCCAGCCCTCGCCAAGCTCCCCGCCGCCCAATCCTGGATCGCCAGCAACATCCTCCCCTTCCACCCCGCCACTCTCATCAACCACATCGCCGTCGGCAACGAGATCCTCGCCACCTCCGATAAGAATCTCATCGCTCACATGCTCCCCGCCATGAAGGCCTTACACACCGCTCTTAAACTCGCCAACATCACCGATATCAAGGTCTCCACTCCACACTCCCTAGGCATTTTGTCTGCCTCTGAACCGCCCAGTGCCGGCCGGTTCCGCCGAGGGTACGACCGGGTCATCTTCGCTCCGATCCTCGAGTTTCACCGCCAGACGCAAACGCCGTTCATGGTGAATCCATATCCATTCTTCGGGTTTAATTCCACGACGTTGAACTACGCTCTGTTCAAGCCCAACACCGGAGTTTTCGACAAGGCCACCGGGAAGCTCTACACGAACATGTTCGACGCGCAGATGGATGCGGTTTACTCGGCGATGAAGAAACTCGGATACGGCGATGTGGACATTGTTGTGGCGGAGACCGGCTGGCCGTCCGTCGGCGAACCGAACCAACCCGGGGTGACCTTGGAGAATGCTATTTCGTTCAACCGGAATCTCGTGAAGCACGTCAGCTCCGGCATGGGTACGCCGATGATGCCGAACCGAACGTTTGAAACTTACGTCTTCTCGCTTTTCAATGAGAATCTCAAACCGTCGGTCTCCGAGCGGAACTACGGTCTGTTTAACCCGGATTTCACTCCGGTCTACGACGTCGGAATCCTCCGGAATGAACCGGTAAACCGCTTTTTCTTCTAGTAAAATCATTTTAGCCTCGCCGGAGTTAGGCAAATCACGGCAGGGGTAGTTTAGTAAATTCATCTGTTAGGAAAGCACGCAAAATTACTAGTCTGTAAGAAGAAGAGTATACGGCATTTCCATTTCCCCAAGAGCTCAGAACACGTGTCAATTTCTTATTCGTTTGTAATTTCCGTTGTTTGTTGACTAATAAAAAACCGACGCGTGGCCGTACGAGTTCCCGACATCAAGCGTGTATTCCACGTGTCCATTTTGTGACAAAAATACCCTCACTTTTCTTTCTACTTCAAGGGCAATGAAGTGAATATGCTTTTTGTTTTTGAATTTGTGTTTTTGACGAATTTTGGATCTTGACCGTTGGGTTTGGTGATGTAACAGTCGAAGGGGCCCACGGCGCCAGGTGGGCAGGCGCCGAAACCGTCCGATGCTGGGCGGAAGTGGTGTGTGCCGAAAACGGACGCTTCAGATGAGGCGTTGCAGAAGAATATTGATTACGTGTGCAGTAGTGGAGTGGACTGTGGGCCCATACAAGCGGGTGGGGCCTGCTACGATCCAAATACGGTGCGGTCGCATGCATCGTATGCCATGGACGCTTATTTTCAGTTTGCTGGACGCCATGATTACAACTGTGATTTCGACCATACTGGCCTTCTCACTAGCACCGACCCCAGTAAGATTTATTTATTTATTTTTTATATTGAATTATTTAGATTATCATTTGCTCTTTGAATTATTATTATTATTATTATTTTGGTCCCAATAGATCTAGATATTTAGTTAACTATACTTTAGTTGTCAATACTTTTTGTTTTGTGTCTGCTTAATATATTAGTTGGAAATTGAGGAATATTTGAAATTAAATTATTATTATTATTATTATAAAGTGATATTAAATAATCTTTTAGAGCGTGTTGGATTTGGTAGAAAAGAATGAAGTGGAGTGGAATGAAAGAATTAATGATACTTGGGATTATTTAATGTTAGTAGGGCTAATTAATTTAAAATTTAAATGTTTTTCAAAATTTCATCCAGAAATGTTATGATAAATATATTTGTTTTAATAAAAAATTAAATGGATTAACAAACTTAATCGATAGGTAAGATTTCTGCAATATTTTAAAGCAAATTGATAAGTTAGTTTTAGTGTTGATTTTCATCTTAAAGCGACACCAACTAATCCATGAACATTTTTATTGTTTGTGGTAATTAATTATTTGTTTGAAAGTTGATGTTAATAATAGTTTAATGCTAAGTTTAATTTAATTAGTTTGATGGAGGATTGTTAAAAGGTGACTGACTGTTTACGACCAATACGTGTAATAAGATTCTAGAAGGGTGATTGTTTGCCATTCTTCTGCCAAACACGAACACACCCTAGATTAGACTTAATTGAAGATTTTAGGTGTATTCGATAGTTAATAGTTTAGTGGCCTATTATTAGACAATTGTGTGTTTTTAGTTCAATAATAATTATTGAGGATATGATCTTTTGGTTAGTGGATATATATTAATGTCACTATAAAATACTAGTTAATTATTGTTTAAAAGAATTTTATTTTCAAATTTGATTAAATCTTACAATCAAATTGTTATTATTATTATTTTTTTTCTTTCTAAACGATGCTTGAAATAACCCCATCTTATTTAGATTGGTTTATTAAATCCGAACTAAATTTGAATATAATATGTCTATAATTTTGGAATTTTACAGGCTACGAGGCCTGTAGTTACCCGTTGGATGCAGAAAAATTAACGGAGAAATCGGTGGCGTCTGCGGCGGCGATGAGGCGGAGGACGATGTCAGATGCGGTGGCGCGTGTAGTTATTGCGGTGGTAATTGGCTGTGTTTTCTTAATATAAATTAGAAATGGTGGAGCTTTGTAGAGTTGATGGGACATGCGTGCAATATCAAGGTTAAATTAAACGTTTGGTAAAATGAATTTTGGGGAACTATATATATATTTTTTCTCTTAAAAA

mRNA sequence

GTGCAAATGTTACCTCGCAAGAATATTCTAAAAGGATTTAAAATAAAAAATGATTAACCTTCAAGAGCAATATATAGCTTATTTGTATATTTTCCAGCGAACCATGGGCCTGTTAATGGGCCTAAAAGAATCCCGAGATGCGTGGGCCCCACCTTCTGCACCACTGCCCCACTCTCTACTTCGAATTTTCACTTTTCAACTTTTCCAGTTTTCCCTCTCCTCAGCGCCAATGGCTTGCTCTCCGTTTCTCTCTCTCCTGCTCTTCTCTCTGCTCCTCCGCCTCTCCGCCGCCGCCTACTCCATCGGCGTCAACTACGGCACCGTCGCCGACAACCTCCCTCCGCCCTCCCAGGTCGCCGCCTTCCTCAAATCCCACACCACCATCGACCGCGTCAAGATCTTCGACGCCAACCCCGACATCCTGCACGCCTTCGCCGGCACCGCCATCTCCCTCACCGTCTCCGTCGCCAACGCCGACATCCCAGCCCTCGCCAAGCTCCCCGCCGCCCAATCCTGGATCGCCAGCAACATCCTCCCCTTCCACCCCGCCACTCTCATCAACCACATCGCCGTCGGCAACGAGATCCTCGCCACCTCCGATAAGAATCTCATCGCTCACATGCTCCCCGCCATGAAGGCCTTACACACCGCTCTTAAACTCGCCAACATCACCGATATCAAGGTCTCCACTCCACACTCCCTAGGCATTTTGTCTGCCTCTGAACCGCCCAGTGCCGGCCGGTTCCGCCGAGGGTACGACCGGGTCATCTTCGCTCCGATCCTCGAGTTTCACCGCCAGACGCAAACGCCGTTCATGGTGAATCCATATCCATTCTTCGGGTTTAATTCCACGACGTTGAACTACGCTCTGTTCAAGCCCAACACCGGAGTTTTCGACAAGGCCACCGGGAAGCTCTACACGAACATGTTCGACGCGCAGATGGATGCGGTTTACTCGGCGATGAAGAAACTCGGATACGGCGATGTGGACATTGTTGTGGCGGAGACCGGCTGGCCGTCCGTCGGCGAACCGAACCAACCCGGGGTGACCTTGGAGAATGCTATTTCGTTCAACCGGAATCTCGTGAAGCACGTCAGCTCCGGCATGGGTACGCCGATGATGCCGAACCGAACGTTTGAAACTTACGTCTTCTCGCTTTTCAATGAGAATCTCAAACCGTCGGTCTCCGAGCGGAACTACGGTCTGTTTAACCCGGATTTCACTCCGGTCTACGACGTCGGAATCCTCCGGAATGAACCGTCGAAGGGGCCCACGGCGCCAGGTGGGCAGGCGCCGAAACCGTCCGATGCTGGGCGGAAGTGGTGTGTGCCGAAAACGGACGCTTCAGATGAGGCGTTGCAGAAGAATATTGATTACGTGTGCAGTAGTGGAGTGGACTGTGGGCCCATACAAGCGGGTGGGGCCTGCTACGATCCAAATACGGTGCGGTCGCATGCATCGTATGCCATGGACGCTTATTTTCAGTTTGCTGGACGCCATGATTACAACTGTGATTTCGACCATACTGGCCTTCTCACTAGCACCGACCCCAGCTACGAGGCCTGTAGTTACCCGTTGGATGCAGAAAAATTAACGGAGAAATCGGTGGCGTCTGCGGCGGCGATGAGGCGGAGGACGATGTCAGATGCGGTGGCGCGTGTAGTTATTGCGGTGGTAATTGGCTGTGTTTTCTTAATATAAATTAGAAATGGTGGAGCTTTGTAGAGTTGATGGGACATGCGTGCAATATCAAGGTTAAATTAAACGTTTGGTAAAATGAATTTTGGGGAACTATATATATATTTTTTCTCTTAAAAA

Coding sequence (CDS)

ATGATTAACCTTCAAGAGCAATATATAGCTTATTTGTATATTTTCCAGCGAACCATGGGCCTGTTAATGGGCCTAAAAGAATCCCGAGATGCGTGGGCCCCACCTTCTGCACCACTGCCCCACTCTCTACTTCGAATTTTCACTTTTCAACTTTTCCAGTTTTCCCTCTCCTCAGCGCCAATGGCTTGCTCTCCGTTTCTCTCTCTCCTGCTCTTCTCTCTGCTCCTCCGCCTCTCCGCCGCCGCCTACTCCATCGGCGTCAACTACGGCACCGTCGCCGACAACCTCCCTCCGCCCTCCCAGGTCGCCGCCTTCCTCAAATCCCACACCACCATCGACCGCGTCAAGATCTTCGACGCCAACCCCGACATCCTGCACGCCTTCGCCGGCACCGCCATCTCCCTCACCGTCTCCGTCGCCAACGCCGACATCCCAGCCCTCGCCAAGCTCCCCGCCGCCCAATCCTGGATCGCCAGCAACATCCTCCCCTTCCACCCCGCCACTCTCATCAACCACATCGCCGTCGGCAACGAGATCCTCGCCACCTCCGATAAGAATCTCATCGCTCACATGCTCCCCGCCATGAAGGCCTTACACACCGCTCTTAAACTCGCCAACATCACCGATATCAAGGTCTCCACTCCACACTCCCTAGGCATTTTGTCTGCCTCTGAACCGCCCAGTGCCGGCCGGTTCCGCCGAGGGTACGACCGGGTCATCTTCGCTCCGATCCTCGAGTTTCACCGCCAGACGCAAACGCCGTTCATGGTGAATCCATATCCATTCTTCGGGTTTAATTCCACGACGTTGAACTACGCTCTGTTCAAGCCCAACACCGGAGTTTTCGACAAGGCCACCGGGAAGCTCTACACGAACATGTTCGACGCGCAGATGGATGCGGTTTACTCGGCGATGAAGAAACTCGGATACGGCGATGTGGACATTGTTGTGGCGGAGACCGGCTGGCCGTCCGTCGGCGAACCGAACCAACCCGGGGTGACCTTGGAGAATGCTATTTCGTTCAACCGGAATCTCGTGAAGCACGTCAGCTCCGGCATGGGTACGCCGATGATGCCGAACCGAACGTTTGAAACTTACGTCTTCTCGCTTTTCAATGAGAATCTCAAACCGTCGGTCTCCGAGCGGAACTACGGTCTGTTTAACCCGGATTTCACTCCGGTCTACGACGTCGGAATCCTCCGGAATGAACCGTCGAAGGGGCCCACGGCGCCAGGTGGGCAGGCGCCGAAACCGTCCGATGCTGGGCGGAAGTGGTGTGTGCCGAAAACGGACGCTTCAGATGAGGCGTTGCAGAAGAATATTGATTACGTGTGCAGTAGTGGAGTGGACTGTGGGCCCATACAAGCGGGTGGGGCCTGCTACGATCCAAATACGGTGCGGTCGCATGCATCGTATGCCATGGACGCTTATTTTCAGTTTGCTGGACGCCATGATTACAACTGTGATTTCGACCATACTGGCCTTCTCACTAGCACCGACCCCAGCTACGAGGCCTGTAGTTACCCGTTGGATGCAGAAAAATTAACGGAGAAATCGGTGGCGTCTGCGGCGGCGATGAGGCGGAGGACGATGTCAGATGCGGTGGCGCGTGTAGTTATTGCGGTGGTAATTGGCTGTGTTTTCTTAATATAA

Protein sequence

MINLQEQYIAYLYIFQRTMGLLMGLKESRDAWAPPSAPLPHSLLRIFTFQLFQFSLSSAPMACSPFLSLLLFSLLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPDILHAFAGTAISLTVSVANADIPALAKLPAAQSWIASNILPFHPATLINHIAVGNEILATSDKNLIAHMLPAMKALHTALKLANITDIKVSTPHSLGILSASEPPSAGRFRRGYDRVIFAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNTGVFDKATGKLYTNMFDAQMDAVYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGTPMMPNRTFETYVFSLFNENLKPSVSERNYGLFNPDFTPVYDVGILRNEPSKGPTAPGGQAPKPSDAGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQFAGRHDYNCDFDHTGLLTSTDPSYEACSYPLDAEKLTEKSVASAAAMRRRTMSDAVARVVIAVVIGCVFLI
Homology
BLAST of Sed0023995 vs. NCBI nr
Match: XP_022948604.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita moschata])

HSP 1 Score: 847.4 bits (2188), Expect = 6.8e-242
Identity = 426/491 (86.76%), Postives = 450/491 (91.65%), Query Frame = 0

Query: 61  MACSPFLSLLLFSLLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 120
           MA S  LSLLLFSL LRLS+A YSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA
Sbjct: 1   MANSLLLSLLLFSLFLRLSSATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60

Query: 121 NPDILHAFAGTAISLTVSVANADIPALAKLPAAQSWIASNILPFHPATLINHIAVGNEIL 180
           NPDIL AFA T I+LTVSVAN DIPALAKLPAAQSWIA+NILPFHP TLINHIAVGNEIL
Sbjct: 61  NPDILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120

Query: 181 ATSDKNLIAHMLPAMKALHTALKLANITDIKVSTPHSLGILSASEPPSAGRFRRGYDRVI 240
           ATSDKNLIAHMLPAMKALH+AL LANITDIKVS+PHSLGILSASEPPS GRFR+GYDR I
Sbjct: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180

Query: 241 FAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNTGVFDKATGKLYTNMFDAQMDA 300
           FAPILEFHR+T+TPFMVNPYPFFGF   TL+YALFKPN G FD  TGK YTNMFDAQMDA
Sbjct: 181 FAPILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240

Query: 301 VYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGTPMMPN 360
           V++AM KLGYGDVDIVVAETGWPS G+PNQPGV+LENAIS+N+NLVKHV+SG GTPMMPN
Sbjct: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPN 300

Query: 361 RTFETYVFSLFNENLKPSVSERNYGLFNPDFTPVYDVGILRNEPSKGPTAPGGQAPKPSD 420
           RTFETYVFSLFNENLKPSVSERNYGLF PD TPVYDVGILRN P+ GPTAP GQAP+PSD
Sbjct: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPTTGPTAPAGQAPQPSD 360

Query: 421 AGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQF 480
            GRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQ 
Sbjct: 361 GGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQS 420

Query: 481 AGRHDYNCDFDHTGLLTSTDPSYEACSYPLDAEKLTEKSVASAAAMRRR-TMSDAVARVV 540
           +GRHD+NCDF+HTG+LTSTDPSY+ACSYPL AEKLTEKSVASAAAMRRR  +  AVAR  
Sbjct: 421 SGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSVASAAAMRRRIRLLGAVARTC 480

Query: 541 IAVVIGCVFLI 551
           IAVV G V LI
Sbjct: 481 IAVVAGGVLLI 491

BLAST of Sed0023995 vs. NCBI nr
Match: KAG6607245.1 (Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 846.3 bits (2185), Expect = 1.5e-241
Identity = 426/491 (86.76%), Postives = 449/491 (91.45%), Query Frame = 0

Query: 61  MACSPFLSLLLFSLLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 120
           MA S  LSLLLFSL LRLS A YSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA
Sbjct: 1   MANSLLLSLLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60

Query: 121 NPDILHAFAGTAISLTVSVANADIPALAKLPAAQSWIASNILPFHPATLINHIAVGNEIL 180
           NPDIL AFA T I+LTVSVAN DIPALAKLPAAQSWIA+NILPFHP TLINHIAVGNEIL
Sbjct: 61  NPDILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120

Query: 181 ATSDKNLIAHMLPAMKALHTALKLANITDIKVSTPHSLGILSASEPPSAGRFRRGYDRVI 240
           ATSDKNLIAHMLPAMKALH+AL LANITDIKVS+PHSLGILSASEPPS GRFR+GYDR I
Sbjct: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180

Query: 241 FAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNTGVFDKATGKLYTNMFDAQMDA 300
           FAPILEFHR+T+TPFMVNPYPFFGF   TL+YALFKPN G FD  TGK YTNMFDAQMDA
Sbjct: 181 FAPILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240

Query: 301 VYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGTPMMPN 360
           V++AM KLGYGDVDIVVAETGWPS G+PNQPGV+LENAIS+N+NLVKHV+SG GTPMMPN
Sbjct: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPN 300

Query: 361 RTFETYVFSLFNENLKPSVSERNYGLFNPDFTPVYDVGILRNEPSKGPTAPGGQAPKPSD 420
           RTFETYVFSLFNENLKPSVSERNYGLF PD TPVYDVGILRN P+ GPTAP GQAP+PSD
Sbjct: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPTTGPTAPAGQAPQPSD 360

Query: 421 AGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQF 480
            GRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQ 
Sbjct: 361 GGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQS 420

Query: 481 AGRHDYNCDFDHTGLLTSTDPSYEACSYPLDAEKLTEKSVASAAAMRRR-TMSDAVARVV 540
           +GRHD+NCDF+HTG+LTSTDPSY+ACSYPL AEKLTEKSVASAAAMRRR  +  AVAR  
Sbjct: 421 SGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSVASAAAMRRRIRLLGAVARTC 480

Query: 541 IAVVIGCVFLI 551
           IAVV G V LI
Sbjct: 481 IAVVAGGVLLI 491

BLAST of Sed0023995 vs. NCBI nr
Match: XP_022998328.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita maxima])

HSP 1 Score: 845.9 bits (2184), Expect = 2.0e-241
Identity = 426/491 (86.76%), Postives = 449/491 (91.45%), Query Frame = 0

Query: 61  MACSPFLSLLLFSLLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 120
           MA S  LSLLL SL LRLS+A +SIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA
Sbjct: 1   MANSLLLSLLLVSLFLRLSSATFSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60

Query: 121 NPDILHAFAGTAISLTVSVANADIPALAKLPAAQSWIASNILPFHPATLINHIAVGNEIL 180
           NPDIL AFAGT I+LTVSVAN DIPALAKLPAAQSWIA+NILPFHP TLINHIAVGNEIL
Sbjct: 61  NPDILRAFAGTGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120

Query: 181 ATSDKNLIAHMLPAMKALHTALKLANITDIKVSTPHSLGILSASEPPSAGRFRRGYDRVI 240
           ATSDKNLIAHMLPAMKALH+AL LANITDIKVS+PHSLGILSASEPPS GRFR+GYDR I
Sbjct: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180

Query: 241 FAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNTGVFDKATGKLYTNMFDAQMDA 300
           FAPILEFHR+T+TPFMVNPYPFFGF   TL YALFKPN G FD  TGK YTNMFDAQMDA
Sbjct: 181 FAPILEFHRKTKTPFMVNPYPFFGFRPATLEYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240

Query: 301 VYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGTPMMPN 360
           V++AM KLGYGDVDIVVAETGWPS G+PNQPGV+LENAIS+NRNLVKHV+SG GTPMMPN
Sbjct: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNRNLVKHVNSGKGTPMMPN 300

Query: 361 RTFETYVFSLFNENLKPSVSERNYGLFNPDFTPVYDVGILRNEPSKGPTAPGGQAPKPSD 420
           RTFETYVFSLFNENLKPSVSERNYGLF PD TPVYDVGILRN P+ GPTAP GQAP+PSD
Sbjct: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPTTGPTAPTGQAPQPSD 360

Query: 421 AGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQF 480
            GRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQ 
Sbjct: 361 GGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQS 420

Query: 481 AGRHDYNCDFDHTGLLTSTDPSYEACSYPLDAEKLTEKSVASAAAMRRR-TMSDAVARVV 540
           +GRHD+NCDF+HTG+LTSTDPSY+ACSYPL AEKLTEKSVASAAAMRRR  +  AVAR  
Sbjct: 421 SGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSVASAAAMRRRIRLLGAVARAC 480

Query: 541 IAVVIGCVFLI 551
           IAVV G V LI
Sbjct: 481 IAVVAGGVLLI 491

BLAST of Sed0023995 vs. NCBI nr
Match: XP_023522990.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 841.6 bits (2173), Expect = 3.7e-240
Identity = 423/491 (86.15%), Postives = 449/491 (91.45%), Query Frame = 0

Query: 61  MACSPFLSLLLFSLLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 120
           MA S  LSLLLFSL LRLS+A YSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA
Sbjct: 1   MANSLLLSLLLFSLFLRLSSATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60

Query: 121 NPDILHAFAGTAISLTVSVANADIPALAKLPAAQSWIASNILPFHPATLINHIAVGNEIL 180
           NPDIL AFA T I+LTVSVAN DIP+LAKLPAAQSWIA+NILPFHP TLINHIAVGNEIL
Sbjct: 61  NPDILRAFAATGIALTVSVANGDIPSLAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120

Query: 181 ATSDKNLIAHMLPAMKALHTALKLANITDIKVSTPHSLGILSASEPPSAGRFRRGYDRVI 240
           ATSDKNLIAHMLPAMKALH+AL LANITDIKVS+PHSLGILSASEPPS GRFR+GYDR I
Sbjct: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180

Query: 241 FAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNTGVFDKATGKLYTNMFDAQMDA 300
           FAPILEFHR+++TPFMVNPYPFFGF   TL+YALFKPN G FD  TGK YTNMFDAQMDA
Sbjct: 181 FAPILEFHRKSKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240

Query: 301 VYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGTPMMPN 360
           V++AM KLGYGDVDIVVAETGWPS G+PNQPGV+LENAIS+NRNLVKHV+SG GTPMMPN
Sbjct: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNRNLVKHVNSGKGTPMMPN 300

Query: 361 RTFETYVFSLFNENLKPSVSERNYGLFNPDFTPVYDVGILRNEPSKGPTAPGGQAPKPSD 420
           RTFETYVFSLFNENLKPSVSERNYGLF PD TPVYDVGILRN P+ GPTAP GQAP+PSD
Sbjct: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPTTGPTAPAGQAPQPSD 360

Query: 421 AGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQF 480
             RKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQ 
Sbjct: 361 GVRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQS 420

Query: 481 AGRHDYNCDFDHTGLLTSTDPSYEACSYPLDAEKLTEKSVASAAAMRRR-TMSDAVARVV 540
           +GRHD+NCDF+HTG+LTSTDPSY+AC+YPL AEKLTEKSVASAAAMRRR  +  AVAR  
Sbjct: 421 SGRHDFNCDFNHTGVLTSTDPSYKACTYPLGAEKLTEKSVASAAAMRRRIRLLGAVARAC 480

Query: 541 IAVVIGCVFLI 551
           IAVV G V LI
Sbjct: 481 IAVVAGGVLLI 491

BLAST of Sed0023995 vs. NCBI nr
Match: KAG7036929.1 (Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 834.3 bits (2154), Expect = 5.9e-238
Identity = 426/511 (83.37%), Postives = 449/511 (87.87%), Query Frame = 0

Query: 61  MACSPFLSLLLFSLLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 120
           MA S  LSLLLFSL LRLS A YSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA
Sbjct: 1   MANSLLLSLLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60

Query: 121 NPDILHAFAGTAISLTVSVANADIPALAKLPAAQSWIASNILPFHPATLINHIAVGNEIL 180
           NPDIL AFA T I+LTVSVAN DIPALAKLPAAQSWIA+NILPFHP TLINHIAVGNEIL
Sbjct: 61  NPDILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120

Query: 181 ATSDKNLIAHMLPAMKALHTALKLANITDIKVSTPHSLGILSASEPPSAGRFRRGYDRVI 240
           ATSDKNLIAHMLPAMKALH+AL LANITDIKVS+PHSLGILSASEPPS GRFR+GYDR I
Sbjct: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180

Query: 241 FAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNTGVFDKATGKLYTNMFDAQMDA 300
           FAPILEFHR+T+TPFMVNPYPFFGF   TL+YALFKPN G FD  TGK YTNMFDAQMDA
Sbjct: 181 FAPILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240

Query: 301 VYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGTPMMPN 360
           V++AM KLGYGDVDIVVAETGWPS G+PNQPGV+LENAIS+N+NLVKHV+SG GTPMMPN
Sbjct: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPN 300

Query: 361 RTFETYVFSLFNENLKPSVSERNYGLFNPDFTPVYDVGILRN------------------ 420
           RTFETYVFSLFNENLKPSVSERNYGLF PD TPVYDVGILRN                  
Sbjct: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGI 360

Query: 421 --EPSKGPTAPGGQAPKPSDAGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGAC 480
              P+ GPTAP GQAP+PSD GRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGAC
Sbjct: 361 MPNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGAC 420

Query: 481 YDPNTVRSHASYAMDAYFQFAGRHDYNCDFDHTGLLTSTDPSYEACSYPLDAEKLTEKSV 540
           YDPNTVRSHASYAMDAYFQ +GRHD+NCDF+HTG+LTSTDPSY+ACSYPL AEKLTEKSV
Sbjct: 421 YDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSV 480

Query: 541 ASAAAMRRR-TMSDAVARVVIAVVIGCVFLI 551
           ASAAAMRRR  +  AVAR  IAVV G V LI
Sbjct: 481 ASAAAMRRRIRLLGAVARTCIAVVAGGVLLI 511

BLAST of Sed0023995 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 1.0e-136
Identity = 250/460 (54.35%), Postives = 321/460 (69.78%), Query Frame = 0

Query: 67  LSLLLFSLLLRLSAA-------AYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFD 126
           + LL+  +LL   AA       +  IGVNYG  ADNLP P+ VA FL + TTIDRVK+FD
Sbjct: 1   MPLLILLMLLAAGAAGAESATPSLHIGVNYGANADNLPSPTSVATFLATKTTIDRVKLFD 60

Query: 127 ANPDILHAFAGTAISLTVSVANADIPALAK----LPAAQSWIASNILPFHPATLINHIAV 186
           ANP  + AFAGT ISL VS+ N+ +PALA     L AA+SWI +N+ P+ PAT +  +  
Sbjct: 61  ANPTFISAFAGTPISLAVSLPNSALPALADKATGLDAARSWIRANLSPYVPATNVTLLLA 120

Query: 187 GNEILATSDKNLIAHMLPAMKALHTALKLANITDIKVSTPHSLGILSASEP-PSAGRFRR 246
           GNEIL ++D NLI  +LPAM+ L  ALK   +T ++V+TPH LGIL+ S+  PS   FR 
Sbjct: 121 GNEILLSTDTNLILSLLPAMRRLAQALKAEGLTGVRVTTPHYLGILAPSDGIPSNASFRA 180

Query: 247 GYDRVIFAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNTGVFDKATGKLYTNMF 306
           GY+  +F  +L+FHR T +PFMVNPYP+F +   TLNYALF+PN+G++D AT   YT+M 
Sbjct: 181 GYNTKLFPAMLQFHRDTGSPFMVNPYPYFSYRPETLNYALFRPNSGIYDPATKLNYTSML 240

Query: 307 DAQMDAVYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMG 366
           DAQMDA+Y+AMKKLGYGDVDI V E GWP+  EP Q GV ++ A  FN  +++  SSG G
Sbjct: 241 DAQMDAIYTAMKKLGYGDVDIAVGEAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKG 300

Query: 367 TPMMPNRTFETYVFSLFNENLKPS-VSERNYGLFNPDFTPVYDVGILRNEPSKGPTAPGG 426
           TP+MPNRTFETY+FSLF+EN KP  ++ER++GLFNPDFTPVYD+G+LR+  S  PT    
Sbjct: 301 TPLMPNRTFETYLFSLFDENQKPGPIAERHFGLFNPDFTPVYDLGLLRDGASVAPTPSPN 360

Query: 427 QAPKPS-----DAGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRS 486
            +P PS       G KWCV K  A+   LQ NI+Y C   VDC PIQ+GGAC+ PN++++
Sbjct: 361 PSPNPSPKPAPSGGGKWCVAKDGANGTDLQNNINYACGF-VDCKPIQSGGACFSPNSLQA 420

Query: 487 HASYAMDAYFQFAGRHDYNCDFDHTGLLTSTDPSYEACSY 509
           HASY M+AY+Q  G  D  CDF  TG++TS+DPSY  C Y
Sbjct: 421 HASYVMNAYYQANGHTDLACDFKGTGIVTSSDPSYGGCKY 459

BLAST of Sed0023995 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 6.2e-113
Identity = 213/461 (46.20%), Postives = 304/461 (65.94%), Query Frame = 0

Query: 61  MACSPFLSLLLFSLLLRL----SAAAYS-IGVNYGTVADNLPPPSQVAAFLKSHTTIDRV 120
           MA +   S LLF + L L    SA + S +GVNYG ++DNLP        LKS TTI +V
Sbjct: 1   MAANVQTSSLLFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKS-TTIQKV 60

Query: 121 KIFDANPDILHAFAGTAISLTVSVANADIPALAKLP-AAQSWIASNILPFHPATLINHIA 180
           ++F A P ++ AFA T + + +   N DIP LA  P  A  ++ SN++ F+PA+ I  I 
Sbjct: 61  RLFGAEPAVIKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAIT 120

Query: 181 VGNEILATSDKNLIAHMLPAMKALHTALKLANI-TDIKVSTPHSLGILSASEPPSAGRFR 240
           VGNE+L + D+ LI+ +LPAM+ +  AL  A++   +KVST H++ +LS S PPS+G F 
Sbjct: 121 VGNEVLTSGDQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFN 180

Query: 241 RGYDRVIFAPILEFHRQTQTPFMVNPYPFFGFNS----TTLNYALFKPNTGVFDKATGKL 300
            G    + A +L+F      PFM++PYP+F + +     TL + LF+PN G  D   G  
Sbjct: 181 PGLGDTMKA-LLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHK 240

Query: 301 YTNMFDAQMDAVYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHV 360
           YTNMFDAQ+DAV+SA+  +G+ D++IVVAETGWP  G+ N+ G +L+NA ++  NL+ H+
Sbjct: 241 YTNMFDAQVDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHL 300

Query: 361 SSGMGTPMMPNRTFETYVFSLFNENLKPSV-SERNYGLFNPDFTPVYDVGILRNEPSKGP 420
            S +GTP+MP ++ +TY+FSL++E+ K    SE+ +GLF PD +  YDVG+L+N  ++ P
Sbjct: 301 KSKVGTPLMPGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKN--TQNP 360

Query: 421 TAPGGQAPKPSDAGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRS 480
           T P    P P  AG  WCVPK   SD+ L  NI+Y C  G+DCGPIQ GGAC++PNTV++
Sbjct: 361 TTPATPTPTPKAAG-SWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNTVKA 420

Query: 481 HASYAMDAYFQFAGRHDYNCDFDHTGLLTSTDPSYEACSYP 510
           HA+Y M+ Y+Q AGR+ +NCDF  T  LT+T+PSY AC++P
Sbjct: 421 HAAYVMNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNFP 456

BLAST of Sed0023995 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 409.1 bits (1050), Expect = 8.1e-113
Identity = 222/483 (45.96%), Postives = 310/483 (64.18%), Query Frame = 0

Query: 67  LSLLLFSLLLRLSAAAYS-----IGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDAN 126
           LS+ ++ LL+ LS    S     IGVNYG VADNLPPPS+    L+S T+I +V+++ A+
Sbjct: 3   LSISIYFLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQS-TSIQKVRLYGAD 62

Query: 127 PDILHAFAGTAISLTVSVANADIPALAKLP-AAQSWIASNILPFHPATLINHIAVGNEIL 186
           P I+ A AGT + + +  AN D+P+LA  P AA  WI SN+LPF+PA+ I  I VGNEIL
Sbjct: 63  PAIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEIL 122

Query: 187 ATSDKNLIAHMLPAMKALHTALKLANI-TDIKVSTPHSLGILSASEPPSAGRFRRGYDRV 246
            ++D NL+  +LPAM+ +  AL+  ++   IKVST +S+ +L +S+PPS+G F  GY   
Sbjct: 123 MSNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG 182

Query: 247 IFAPILEFHRQTQTPFMVNPYPFFGFNS----TTLNYALFKPNTGVFDKATGKLYTNMFD 306
           +   IL+F   T +PF +NPYPFF + S     TL + LF+PN G  D  TG  YTNMFD
Sbjct: 183 L-KGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFD 242

Query: 307 AQMDAVYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGT 366
           AQ+DAV+SA+K +G+  V+IVVAETGW S G+ N+ G +++NA ++N NL+ H+ S +GT
Sbjct: 243 AQVDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGT 302

Query: 367 PMMPNRTFETYVFSLFNENLKPS-VSERNYGLFNPDFTPVYDVGILRNEPSKGPTAPGGQ 426
           P+MP +  +TY+F+L++ENLKP   SER +GLF  D + VYDVG+ ++  S        Q
Sbjct: 303 PLMPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKSSSS-------SQ 362

Query: 427 APKPSDAGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAM 486
            P        WCVPK  A++E LQ ++D+ C  G+DCG IQ GGAC++PN V SHA+YAM
Sbjct: 363 TPSGKVTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAM 422

Query: 487 DAYFQFAGRHDYNCDFDHTGLLTSTDPSYEACSYPLDAEKLTEKSVASAAAMRRRTMSDA 538
           + YFQ + +   +CDF  T  +TS +PSY  C YP            S A M +   SD 
Sbjct: 423 NMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGGGGGGGGGGGGSKAVMNKYVSSDK 476

BLAST of Sed0023995 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.3e-89
Identity = 190/471 (40.34%), Postives = 278/471 (59.02%), Query Frame = 0

Query: 66  FLSLLLFSLLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPDIL 125
           F+S+L F   L    A+  IG+ YG  ADNLP P++V+  ++ H  I  V+I+DAN D+L
Sbjct: 12  FVSILAF---LNFGMAS-KIGICYGRNADNLPSPNRVSELIQ-HLNIKFVRIYDANIDVL 71

Query: 126 HAFAGTAISLTVSVANADIPALAKLPA-AQSWIASNILPFHPATLINHIAVGNEILATSD 185
            AFA T I L + V NAD+ A A+  +   +W+++NILP++P+T I  I+VG E+    D
Sbjct: 72  KAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPD 131

Query: 186 KNLIAHMLPAMKALHTALKLANI-TDIKVSTPHSLGILSASEPPSAGRFRRGYDRVIFAP 245
            N    +LPAM+ +HTALK + +   IK+S+ HSL ILS S PPS+  F + +      P
Sbjct: 132 -NATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHS-AFLKP 191

Query: 246 ILEFHRQTQTPFMVNPYPFFGFNSTT----LNYALFKPNTGVFDKATGKLYTNMFDAQMD 305
           +LEF  + ++PFM++ YP++ +  +T    L YALF+ ++ V D ATG LY+NMFDAQ+D
Sbjct: 192 MLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLD 251

Query: 306 AVYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGTPMMP 365
           A+Y A+  + +  V ++V E+GWPS G P +   T ENA+++N NL++HV    GTP  P
Sbjct: 252 AIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKP 311

Query: 366 NRTFETYVFSLFNENLKPSV-SERNYGLFNPDFTPVYDVGIL-RNEPSKGPT-APGGQAP 425
               + Y+FSLFNEN KP + SERN+G+F  + T VY +     N     PT +  G +P
Sbjct: 312 GEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSP 371

Query: 426 KPSDA------------------GRKWCVPKTDASDEALQKNIDYVCSSG-VDCGPIQAG 485
            PS +                   +KWC+  + AS   LQ  +D+ C  G VDC  +Q  
Sbjct: 372 SPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPD 431

Query: 486 GACYDPNTVRSHASYAMDAYFQFAGRHDYNCDFDHTGLLTSTDPSYEACSY 509
             C++P+TV SHASYA + Y+Q +G    +C F+   +    DPSY  C Y
Sbjct: 432 QPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of Sed0023995 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 4.3e-82
Identity = 178/469 (37.95%), Postives = 267/469 (56.93%), Query Frame = 0

Query: 66  FLSLLLFSLLLRLSAAAY--SIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPD 125
           F  +   S+LL L    Y   +GV YG  AD+LP PS+V   ++ H  I  V+I+D N  
Sbjct: 5   FKLIFSISILLLLLDCCYGGKVGVCYGRSADDLPTPSKVVQLIQQH-NIKYVRIYDYNSQ 64

Query: 126 ILHAFAGTAISLTVSVANADIPALAKLPA-AQSWIASNILPFHPATLINHIAVGNEILAT 185
           +L AF  T+I L + V N+D+ A ++  +   +W+ +++LP++P T I +I VG E    
Sbjct: 65  VLKAFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDD 124

Query: 186 SDKNLIAHMLPAMKALHTALKLANIT-DIKVSTPHSLGILSASEPPSAGRFRRGYDRVIF 245
              N  + ++PAM+ + TAL+   ++  IKVST  SLGILS S PPSAG F   Y     
Sbjct: 125 PHINASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSY-AYFL 184

Query: 246 APILEFHRQTQTPFMVNPYPFFGF----NSTTLNYALFKPNTGVFDKATGKLYTNMFDAQ 305
            P+LEF  + ++PFM++ YP++ +    N+ +L+Y LF+ ++ V D  TG LY NMFDAQ
Sbjct: 185 RPMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQ 244

Query: 306 MDAVYSAMKKLGYGDVDIVVAETGWPSVGEPNQ-PGVTLENAISFNRNLVKHVSSGMGTP 365
           +DA+Y A+  L +  + I+V ETGWP+ G P +    + +NA ++N N+++HV +  GTP
Sbjct: 245 VDALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTP 304

Query: 366 MMPNRTFETYVFSLFNENLKPSV-SERNYGLFNPDFTPVYDVGILRNEPSKGPTAPGGQA 425
             P      Y+FSLFNEN K  + SERN+GLF PD T VY +            + G  +
Sbjct: 305 AKPGEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDFTGKSNGFHSNSSGTNS 364

Query: 426 PKPSDAGRKWCVPKTDASDEALQKNIDYVCSSG-VDCGPIQAGGACYDPNTVRSHASYAM 485
              S +   WC+  + AS+  L+  +D+ C  G VDC  IQ    C+ P+T+ SHAS+  
Sbjct: 365 ---SGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVF 424

Query: 486 DAYFQFAGRHDYNCDFDHTGLLTSTDPSYEACSYPLDAEKLTEKSVASA 524
           ++YFQ     D  C F   G+  + DPSY+ C Y       T+ + A+A
Sbjct: 425 NSYFQQNRATDVACSFGGAGVKVNKDPSYDKCIYITAGGNKTKATNATA 468

BLAST of Sed0023995 vs. ExPASy TrEMBL
Match: A0A6J1GAD3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111452230 PE=3 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 3.3e-242
Identity = 426/491 (86.76%), Postives = 450/491 (91.65%), Query Frame = 0

Query: 61  MACSPFLSLLLFSLLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 120
           MA S  LSLLLFSL LRLS+A YSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA
Sbjct: 1   MANSLLLSLLLFSLFLRLSSATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60

Query: 121 NPDILHAFAGTAISLTVSVANADIPALAKLPAAQSWIASNILPFHPATLINHIAVGNEIL 180
           NPDIL AFA T I+LTVSVAN DIPALAKLPAAQSWIA+NILPFHP TLINHIAVGNEIL
Sbjct: 61  NPDILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120

Query: 181 ATSDKNLIAHMLPAMKALHTALKLANITDIKVSTPHSLGILSASEPPSAGRFRRGYDRVI 240
           ATSDKNLIAHMLPAMKALH+AL LANITDIKVS+PHSLGILSASEPPS GRFR+GYDR I
Sbjct: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180

Query: 241 FAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNTGVFDKATGKLYTNMFDAQMDA 300
           FAPILEFHR+T+TPFMVNPYPFFGF   TL+YALFKPN G FD  TGK YTNMFDAQMDA
Sbjct: 181 FAPILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240

Query: 301 VYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGTPMMPN 360
           V++AM KLGYGDVDIVVAETGWPS G+PNQPGV+LENAIS+N+NLVKHV+SG GTPMMPN
Sbjct: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPN 300

Query: 361 RTFETYVFSLFNENLKPSVSERNYGLFNPDFTPVYDVGILRNEPSKGPTAPGGQAPKPSD 420
           RTFETYVFSLFNENLKPSVSERNYGLF PD TPVYDVGILRN P+ GPTAP GQAP+PSD
Sbjct: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPTTGPTAPAGQAPQPSD 360

Query: 421 AGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQF 480
            GRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQ 
Sbjct: 361 GGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQS 420

Query: 481 AGRHDYNCDFDHTGLLTSTDPSYEACSYPLDAEKLTEKSVASAAAMRRR-TMSDAVARVV 540
           +GRHD+NCDF+HTG+LTSTDPSY+ACSYPL AEKLTEKSVASAAAMRRR  +  AVAR  
Sbjct: 421 SGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSVASAAAMRRRIRLLGAVARTC 480

Query: 541 IAVVIGCVFLI 551
           IAVV G V LI
Sbjct: 481 IAVVAGGVLLI 491

BLAST of Sed0023995 vs. ExPASy TrEMBL
Match: A0A6J1KGF2 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111492999 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 9.6e-242
Identity = 426/491 (86.76%), Postives = 449/491 (91.45%), Query Frame = 0

Query: 61  MACSPFLSLLLFSLLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 120
           MA S  LSLLL SL LRLS+A +SIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA
Sbjct: 1   MANSLLLSLLLVSLFLRLSSATFSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60

Query: 121 NPDILHAFAGTAISLTVSVANADIPALAKLPAAQSWIASNILPFHPATLINHIAVGNEIL 180
           NPDIL AFAGT I+LTVSVAN DIPALAKLPAAQSWIA+NILPFHP TLINHIAVGNEIL
Sbjct: 61  NPDILRAFAGTGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120

Query: 181 ATSDKNLIAHMLPAMKALHTALKLANITDIKVSTPHSLGILSASEPPSAGRFRRGYDRVI 240
           ATSDKNLIAHMLPAMKALH+AL LANITDIKVS+PHSLGILSASEPPS GRFR+GYDR I
Sbjct: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180

Query: 241 FAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNTGVFDKATGKLYTNMFDAQMDA 300
           FAPILEFHR+T+TPFMVNPYPFFGF   TL YALFKPN G FD  TGK YTNMFDAQMDA
Sbjct: 181 FAPILEFHRKTKTPFMVNPYPFFGFRPATLEYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240

Query: 301 VYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGTPMMPN 360
           V++AM KLGYGDVDIVVAETGWPS G+PNQPGV+LENAIS+NRNLVKHV+SG GTPMMPN
Sbjct: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNRNLVKHVNSGKGTPMMPN 300

Query: 361 RTFETYVFSLFNENLKPSVSERNYGLFNPDFTPVYDVGILRNEPSKGPTAPGGQAPKPSD 420
           RTFETYVFSLFNENLKPSVSERNYGLF PD TPVYDVGILRN P+ GPTAP GQAP+PSD
Sbjct: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPTTGPTAPTGQAPQPSD 360

Query: 421 AGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQF 480
            GRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQ 
Sbjct: 361 GGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQS 420

Query: 481 AGRHDYNCDFDHTGLLTSTDPSYEACSYPLDAEKLTEKSVASAAAMRRR-TMSDAVARVV 540
           +GRHD+NCDF+HTG+LTSTDPSY+ACSYPL AEKLTEKSVASAAAMRRR  +  AVAR  
Sbjct: 421 SGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSVASAAAMRRRIRLLGAVARAC 480

Query: 541 IAVVIGCVFLI 551
           IAVV G V LI
Sbjct: 481 IAVVAGGVLLI 491

BLAST of Sed0023995 vs. ExPASy TrEMBL
Match: A0A5D3BDL6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G002220 PE=3 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 6.9e-232
Identity = 409/516 (79.26%), Postives = 452/516 (87.60%), Query Frame = 0

Query: 41  HSLLRIFTFQLFQFSLSSAPMACSPFLS-LLLFSLLLRLSAAAYSIGVNYGTVADNLPPP 100
           HS++ +F F         +PMA S FLS LLLF   + LS+AAYSIGVNYGTVA+NLPPP
Sbjct: 25  HSVISLFLF--------PSPMAYSLFLSLLLLFFFFIPLSSAAYSIGVNYGTVANNLPPP 84

Query: 101 SQVAAFLKSHTTIDRVKIFDANPDILHAFAGTAISLTVSVANADIPALAKLPAAQSWIAS 160
           +QVAAFLKS T+IDRVKIFDANPDIL AFAGT I+LTVS+AN DIPALAKLPAAQSW+A+
Sbjct: 85  TQVAAFLKSRTSIDRVKIFDANPDILRAFAGTGIALTVSIANGDIPALAKLPAAQSWVAN 144

Query: 161 NILPFHPATLINHIAVGNEILATSDKNLIAHMLPAMKALHTALKLANITDIKVSTPHSLG 220
           NILPFHP TLINHIAVGNEILATSDKNLIAH+LPAMKA+H+ALKLANI+D+KVSTPHSLG
Sbjct: 145 NILPFHPTTLINHIAVGNEILATSDKNLIAHLLPAMKAIHSALKLANISDVKVSTPHSLG 204

Query: 221 ILSASEPPSAGRFRRGYDRVIFAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNT 280
           ILSASEPPS GRFRRGYDR IFAPIL+FH QT+TPFMVNPYPFFGF   TL+YALFKPN 
Sbjct: 205 ILSASEPPSTGRFRRGYDRAIFAPILDFHNQTKTPFMVNPYPFFGFRPATLDYALFKPNA 264

Query: 281 GVFDKATGKLYTNMFDAQMDAVYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAI 340
           GVFD ATGK YTNMFDAQ+DAV+SAMKK+G+GDVDIVVAETGWPS G+PNQPGV++ENAI
Sbjct: 265 GVFDNATGKHYTNMFDAQLDAVHSAMKKVGFGDVDIVVAETGWPSAGDPNQPGVSMENAI 324

Query: 341 SFNRNLVKHVSSGMGTPMMPNRTFETYVFSLFNENLKPSVSERNYGLFNPDFTPVYDVGI 400
           S+NRNLVKHV+SG GTP+MPNRTFETY+FSLFNENLKP+ SERNYGLF PDFTPVYDVGI
Sbjct: 325 SYNRNLVKHVNSGKGTPLMPNRTFETYIFSLFNENLKPTTSERNYGLFQPDFTPVYDVGI 384

Query: 401 LRNEPSKGPTAPGGQAPKPSDAGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGA 460
           LRN P+ GPT+P G  PKPSD GR+WCVPKTDASDEALQKNIDYVCSSGVDCGPIQ GGA
Sbjct: 385 LRNPPTMGPTSPVG-GPKPSDEGRRWCVPKTDASDEALQKNIDYVCSSGVDCGPIQEGGA 444

Query: 461 CYDPNTVRSHASYAMDAYFQFAGRHDYNCDFDHTGLLTSTDPSYEACSYPLDAEKLTEKS 520
           CYDPNTVRSHASYAM+AYFQ AGRHD+NCDF+HT +LT TDPSYEACSYP D EKL EKS
Sbjct: 445 CYDPNTVRSHASYAMNAYFQTAGRHDFNCDFNHTAILTFTDPSYEACSYPFDEEKLAEKS 504

Query: 521 VASAAAMR-----RRTMSDAVARVVIAVVIGCVFLI 551
           VASAA +R        ++ AVAR  IAVV G V LI
Sbjct: 505 VASAATIRCSMVGAVAVAVAVARAFIAVVAGGVLLI 531

BLAST of Sed0023995 vs. ExPASy TrEMBL
Match: A0A5A7VQ76 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold352G00610 PE=3 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 6.9e-232
Identity = 410/522 (78.54%), Postives = 454/522 (86.97%), Query Frame = 0

Query: 41  HSLLRIFTFQLFQFSLSSAPMACSPFLSLLL-------FSLLLRLSAAAYSIGVNYGTVA 100
           HS++ +F F         +PMA S FLSLLL       F   + LS+AAYSIGVNYGTVA
Sbjct: 44  HSVISLFLF--------PSPMAYSLFLSLLLFFFFFFFFFFFIPLSSAAYSIGVNYGTVA 103

Query: 101 DNLPPPSQVAAFLKSHTTIDRVKIFDANPDILHAFAGTAISLTVSVANADIPALAKLPAA 160
           +NLPPP+QVAAFLKS T+IDRVKIFDANPDIL AFAGT I+LTVS+AN DIPALAKLPAA
Sbjct: 104 NNLPPPTQVAAFLKSRTSIDRVKIFDANPDILRAFAGTGIALTVSIANGDIPALAKLPAA 163

Query: 161 QSWIASNILPFHPATLINHIAVGNEILATSDKNLIAHMLPAMKALHTALKLANITDIKVS 220
           QSW+A+NILPFHP TLINHIAVGNEILATSDKNLIAH+LPAMKA+H+ALKLANI+D+KVS
Sbjct: 164 QSWVANNILPFHPTTLINHIAVGNEILATSDKNLIAHLLPAMKAIHSALKLANISDVKVS 223

Query: 221 TPHSLGILSASEPPSAGRFRRGYDRVIFAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYA 280
           TPHSLGILSASEPPSAGRFRRGYDR IFAPIL+FH QT+TPFMVNPYPFFGF   TL+YA
Sbjct: 224 TPHSLGILSASEPPSAGRFRRGYDRAIFAPILDFHNQTKTPFMVNPYPFFGFRPATLDYA 283

Query: 281 LFKPNTGVFDKATGKLYTNMFDAQMDAVYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGV 340
           LFKPN GVFD ATGK YTNMFDAQ+DAV+SAMKK+G+GDVDIVVAETGWPS G+PNQPGV
Sbjct: 284 LFKPNAGVFDNATGKHYTNMFDAQLDAVHSAMKKVGFGDVDIVVAETGWPSAGDPNQPGV 343

Query: 341 TLENAISFNRNLVKHVSSGMGTPMMPNRTFETYVFSLFNENLKPSVSERNYGLFNPDFTP 400
           ++ENAIS+NRNLVKHV+SG GTP+MPNRTFETY+FSLFNENLKP+ SERNYGLF PDFTP
Sbjct: 344 SMENAISYNRNLVKHVNSGKGTPLMPNRTFETYIFSLFNENLKPTTSERNYGLFQPDFTP 403

Query: 401 VYDVGILRNEPSKGPTAPGGQAPKPSDAGRKWCVPKTDASDEALQKNIDYVCSSGVDCGP 460
           VYDVGILRN P+ GPT+P G+ PKPSD GR+WCVPKTDASDEALQKNIDYVCSSGVDCGP
Sbjct: 404 VYDVGILRNPPTMGPTSPVGE-PKPSDEGRRWCVPKTDASDEALQKNIDYVCSSGVDCGP 463

Query: 461 IQAGGACYDPNTVRSHASYAMDAYFQFAGRHDYNCDFDHTGLLTSTDPSYEACSYPLDAE 520
           IQ GGACYDPNTVRSHASYAM+AYFQ AGRHD+NCDF+HT +LT TDPSYEACSYP D E
Sbjct: 464 IQEGGACYDPNTVRSHASYAMNAYFQTAGRHDFNCDFNHTAILTFTDPSYEACSYPFDEE 523

Query: 521 KLTEKSVASAAAMR-----RRTMSDAVARVVIAVVIGCVFLI 551
           KL EKSVASAA +R        ++ AVAR  IAVV G V LI
Sbjct: 524 KLAEKSVASAATIRCSMVGAVAVAVAVARAFIAVVAGGVLLI 556

BLAST of Sed0023995 vs. ExPASy TrEMBL
Match: A0A0A0LVC1 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G522000 PE=3 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 3.8e-230
Identity = 404/491 (82.28%), Postives = 443/491 (90.22%), Query Frame = 0

Query: 61  MACSPFLSLLLFSLLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 120
           MA S F SLLLF   + LS+AAYSIGVNYGTVA+NLPPPSQVAAFLKSHT+IDRVKIFDA
Sbjct: 1   MAYSLFFSLLLF-FFIPLSSAAYSIGVNYGTVANNLPPPSQVAAFLKSHTSIDRVKIFDA 60

Query: 121 NPDILHAFAGTAISLTVSVANADIPALAKLPAAQSWIASNILPFHPATLINHIAVGNEIL 180
           NPDIL AFAGT I+LTVSVAN DIPALAKLPAAQSW+A+NILPFHP TLINHIAVGNEIL
Sbjct: 61  NPDILRAFAGTGIALTVSVANGDIPALAKLPAAQSWVANNILPFHPTTLINHIAVGNEIL 120

Query: 181 ATSDKNLIAHMLPAMKALHTALKLANITDIKVSTPHSLGILSASEPPSAGRFRRGYDRVI 240
           ATSDKNLIAH+LPAMKA+H+ALKLA+I+D+KVSTPHSLGILSASEPPS GRFRRGYDR I
Sbjct: 121 ATSDKNLIAHLLPAMKAIHSALKLAHISDVKVSTPHSLGILSASEPPSTGRFRRGYDRAI 180

Query: 241 FAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNTGVFDKATGKLYTNMFDAQMDA 300
           FAPIL+FH QT+TPFMVNPYP+FGF   TL+YALFKPN GVFD ATGK YTNMFDAQ+DA
Sbjct: 181 FAPILDFHNQTKTPFMVNPYPYFGFRPATLDYALFKPNPGVFDNATGKHYTNMFDAQLDA 240

Query: 301 VYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGTPMMPN 360
           VYSAMKK+GYGDVDIVVAETGWPS G+PNQ GV++ENAIS+NRNLVKHV+SG GTP+MPN
Sbjct: 241 VYSAMKKVGYGDVDIVVAETGWPSAGDPNQAGVSMENAISYNRNLVKHVNSGKGTPLMPN 300

Query: 361 RTFETYVFSLFNENLKPSVSERNYGLFNPDFTPVYDVGILRNEPSKGPTAPGGQAPKPSD 420
           RTFETY+FSLFNENLKPS SERNYGLF PDFTPVYDVGIL+N P+ GPT+P G  PKPSD
Sbjct: 301 RTFETYIFSLFNENLKPSTSERNYGLFQPDFTPVYDVGILQNPPAMGPTSPVG-GPKPSD 360

Query: 421 AGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQF 480
             R+WCVPKTDASDEALQKNIDYVCSSGVDCGPIQ GGACYDPNTVRSHASYAM+AYFQ 
Sbjct: 361 EEREWCVPKTDASDEALQKNIDYVCSSGVDCGPIQEGGACYDPNTVRSHASYAMNAYFQT 420

Query: 481 AGRHDYNCDFDHTGLLTSTDPSYEACSYPLDAEKLTEKSVASAAAMRRRTM-SDAVARVV 540
           AGRH++NCDF+HT +LTSTDPSYEACSYP D EKLTEKSVAS A +R R + + A+AR  
Sbjct: 421 AGRHEFNCDFNHTAILTSTDPSYEACSYPFDEEKLTEKSVASGATIRCRMVGAVALARAC 480

Query: 541 IAVVIGCVFLI 551
           IAVV G V LI
Sbjct: 481 IAVVAGGVLLI 489

BLAST of Sed0023995 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 491.1 bits (1263), Expect = 1.2e-138
Identity = 239/450 (53.11%), Postives = 322/450 (71.56%), Query Frame = 0

Query: 65  PFLSLLLFS----LLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 124
           PF SL +F+    LL+  + +  SIGVNYGT+A+NLPPP QVA FL   T I+R+++FD 
Sbjct: 6   PFFSLSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDT 65

Query: 125 NPDILHAFAGTAISLTVSVANADIPALAKLPAAQSWIASNILPFHPATLINHIAVGNEIL 184
           +P IL AFA T I++TV+V N  IP L  L +A+ WI+ +I P  P+T I  I VGNE++
Sbjct: 66  DPQILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVI 125

Query: 185 ATSDKNLIAHMLPAMKALHTALKLANI-TDIKVSTPHSLGILSASEPPSAGRFRRGYDRV 244
           +T+D  LI  ++PAM++LHTAL  A++   I++STPHSLG L+ S PPS+ +FRRGYD  
Sbjct: 126 STADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQ 185

Query: 245 IFAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNTGVFDKATGKLYTNMFDAQMD 304
           +  P+L F R T +PF+VNPYPFFG++  TL++ALF+PN G+FD+ T  LYTNM DAQ+D
Sbjct: 186 VLKPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLD 245

Query: 305 AVYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGTPMMP 364
           +VYSAM KLG+ DV+IV+ E GWPS G+ +Q GV ++ A  FN+NL+  V SG GTP+MP
Sbjct: 246 SVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMP 305

Query: 365 NRTFETYVFSLFNENLKPS-VSERNYGLFNPDFTPVYDVGILRNEPSKGPTAPGGQAPKP 424
           NRTFETY+F+LFNENLK    SERN+G+F  D TP+YD+GILR            ++P  
Sbjct: 306 NRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDPVYNPRSPVR 365

Query: 425 SDAGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYF 484
             + ++WCV K  A   ALQ+NIDYVC  G+DC PI  GG CY PNTV++H+ YAM+ Y+
Sbjct: 366 GSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAMNLYY 425

Query: 485 QFAGRHDYNCDFDHTGLLTSTDPSYEACSY 509
           Q   +H+++CDFD+TG +T+ DPSY  C Y
Sbjct: 426 QTMEKHEFDCDFDNTGEITTIDPSYGNCEY 455

BLAST of Sed0023995 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 484.2 bits (1245), Expect = 1.4e-136
Identity = 236/444 (53.15%), Postives = 319/444 (71.85%), Query Frame = 0

Query: 65  PFLSLLLFS----LLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 124
           PF SL +F+    LL+  + +  SIGVNYGT+A+NLPPP QVA FL   T I+R+++FD 
Sbjct: 6   PFFSLSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDT 65

Query: 125 NPDILHAFAGTAISLTVSVANADIPALAKLPAAQSWIASNILPFHPATLINHIAVGNEIL 184
           +P IL AFA T I++TV+V N  IP L  L +A+ WI+ +I P  P+T I  I VGNE++
Sbjct: 66  DPQILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVI 125

Query: 185 ATSDKNLIAHMLPAMKALHTALKLANI-TDIKVSTPHSLGILSASEPPSAGRFRRGYDRV 244
           +T+D  LI  ++PAM++LHTAL  A++   I++STPHSLG L+ S PPS+ +FRRGYD  
Sbjct: 126 STADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQ 185

Query: 245 IFAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNTGVFDKATGKLYTNMFDAQMD 304
           +  P+L F R T +PF+VNPYPFFG++  TL++ALF+PN G+FD+ T  LYTNM DAQ+D
Sbjct: 186 VLKPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLD 245

Query: 305 AVYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGTPMMP 364
           +VYSAM KLG+ DV+IV+ E GWPS G+ +Q GV ++ A  FN+NL+  V SG GTP+MP
Sbjct: 246 SVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMP 305

Query: 365 NRTFETYVFSLFNENLKPS-VSERNYGLFNPDFTPVYDVGILRNEPSKGPTAPGGQAPKP 424
           NRTFETY+F+LFNENLK    SERN+G+F  D TP+YD+GILR            ++P  
Sbjct: 306 NRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDPVYNPRSPVR 365

Query: 425 SDAGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYF 484
             + ++WCV K  A   ALQ+NIDYVC  G+DC PI  GG CY PNTV++H+ YAM+ Y+
Sbjct: 366 GSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAMNLYY 425

Query: 485 QFAGRHDYNCDFDHTGLLTSTDPS 503
           Q   +H+++CDFD+TG +T+ DPS
Sbjct: 426 QTMEKHEFDCDFDNTGEITTIDPS 449

BLAST of Sed0023995 vs. TAIR 10
Match: AT3G55430.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 472.2 bits (1214), Expect = 5.5e-133
Identity = 247/457 (54.05%), Postives = 324/457 (70.90%), Query Frame = 0

Query: 56  LSSAPMACSPFLSLLLFSLLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRV 115
           ++ AP + S  L LL  ++ L + A   +IGVNYGT+  NLPPP+QVA F+K+ T+ID V
Sbjct: 1   MAKAPPSIS-LLLLLCAAVFLTIPAVISAIGVNYGTLG-NLPPPTQVANFIKTQTSIDSV 60

Query: 116 KIFDANPDILHAFAGTAISLTVSVANADIPALAKLPAAQSWIASNILPFHPATLINHIAV 175
           KIFD NPDIL AFAGT IS+ V+V N DIPALA    A+ W++ NILPFHP T I +I+V
Sbjct: 61  KIFDVNPDILRAFAGTGISVVVTVPNGDIPALANGRQARRWVSVNILPFHPQTKIKYISV 120

Query: 176 GNEILATSDKNLIAHMLPAMKALHTALKLANITDIKVSTPHSLGILS--ASEPPSAGRFR 235
           GNEIL T D N+I ++LPAM+ L+ AL  A + D+KV+T HSL I++   +  PS+GRFR
Sbjct: 121 GNEILLTGDNNMINNLLPAMRNLNNALVRAGVRDVKVTTAHSLNIIAYDLTGAPSSGRFR 180

Query: 236 RGYDRVIFAPILEFHRQTQTPFMVNPYPFFGFNSTTLNYALFK-PNTGVFDKATGKLYTN 295
            G+D+ I APIL +HR+T++PFMVNPYP+FGF+   +N+A+F+ P   V D  T  +YTN
Sbjct: 181 PGWDKGILAPILAYHRRTKSPFMVNPYPYFGFDPKNVNFAIFRTPYKAVRDPFTRHVYTN 240

Query: 296 MFDAQMDAVYSAMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSG 355
           MFDA MD+ YSAMK LGYGDV+IVV ETGWPS  +   P  +  NA  FN N++K  + G
Sbjct: 241 MFDALMDSTYSAMKALGYGDVNIVVGETGWPSACD--APWCSPANAAWFNLNIIKR-AQG 300

Query: 356 MGTPMMPNRTFETYVFSLFNENLKPS-VSERNYGLFNPDFTPVYDVGILRNEPSKGPTAP 415
            GTP+MPNR FETY+F LFNE  KP   +ERN+GLF  DF+PVYDVG+LRN    G    
Sbjct: 301 QGTPLMPNRRFETYIFGLFNEEGKPGPTAERNWGLFRADFSPVYDVGLLRNGQGGGGRP- 360

Query: 416 GGQAPKPSDAGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHAS 475
               P PS AG KWCV ++ A++  LQ +I++VC  GVDC PIQAGG+C++P+++R+HAS
Sbjct: 361 --ALPAPSTAGGKWCVARSGATNTQLQDSINWVCGQGVDCKPIQAGGSCFNPSSLRTHAS 420

Query: 476 YAMDAYFQFAGRHDYNCDFDHTGLLTSTDPSYEACSY 509
           + M+AYFQ  GR D  C+F  TG++   +PS  AC Y
Sbjct: 421 FVMNAYFQSHGRTDGACNFSGTGMIVGNNPSNGACKY 449

BLAST of Sed0023995 vs. TAIR 10
Match: AT3G46570.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 448.4 bits (1152), Expect = 8.6e-126
Identity = 227/338 (67.16%), Postives = 275/338 (81.36%), Query Frame = 0

Query: 67  LSLLLFSLLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPDILH 126
           L  L+ +L+L  S AAY +GVNYGTVA+NLPPP QV  F+K+ T I+ VKIFD N DIL 
Sbjct: 10  LYFLILTLILPFS-AAYQVGVNYGTVANNLPPPHQVVNFIKTKTIINHVKIFDTNHDILA 69

Query: 127 AFAG-TAISLTVSVANADIPALAKLPAAQSWIASNILPFHPATLINHIAVGNEILATSDK 186
           AF+G T ISLTV+V N+DI +L+KL  A+SW++ N+LPF   T I +IAVGNE++ATSDK
Sbjct: 70  AFSGITGISLTVTVPNSDIISLSKLSNARSWLSDNLLPFLLTTSIRYIAVGNEVVATSDK 129

Query: 187 NLIAHMLPAMKALHTALKLANITDIKVSTPHSLGILS-ASEPPSAGRFRRGYDRVIFAPI 246
            LI H+LPAM+ L  AL LAN++ I VSTPHSLGILS +SEPPS+G+FR+GYD+ IF+PI
Sbjct: 130 TLITHLLPAMETLTLALHLANVSRILVSTPHSLGILSGSSEPPSSGKFRKGYDKAIFSPI 189

Query: 247 LEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNTGVF-DKATGKLYTNMFDAQMDAVYS 306
           L+FH +T++PFMVNPYP+FGF   TLNYALF  N  V+ D  T   YTNMFDAQ+DAVYS
Sbjct: 190 LDFHNRTKSPFMVNPYPYFGFGPETLNYALFNTNDVVYVDPVTKLNYTNMFDAQLDAVYS 249

Query: 307 AMKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGTPMMPNRTF 366
           AMK+ GYGDVDIVVAETGWPS GEPNQ GV L+ A ++N NL+KHV+SG GTP+MPNR F
Sbjct: 250 AMKRFGYGDVDIVVAETGWPSAGEPNQTGVGLDYAAAYNGNLIKHVNSGKGTPLMPNRVF 309

Query: 367 ETYVFSLFNENLKPSVSERNYGLFNPDFTPVYDVGILR 402
           ETYVFSLFNENLK SVSE+N+GLF PDFTPVYDVGI++
Sbjct: 310 ETYVFSLFNENLKSSVSEQNFGLFKPDFTPVYDVGIMK 346

BLAST of Sed0023995 vs. TAIR 10
Match: AT2G39640.1 (glycosyl hydrolase family 17 protein )

HSP 1 Score: 418.7 bits (1075), Expect = 7.3e-117
Identity = 215/450 (47.78%), Postives = 303/450 (67.33%), Query Frame = 0

Query: 65  PFLSLLLFSLLLRLSAAAYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPDI 124
           PFL L++  ++ +LSA   +IG+NYGT+  NL PP QV  F+K+ TT D VKI+DANPDI
Sbjct: 11  PFL-LIVAGVIFQLSAVTSAIGINYGTLG-NLQPPQQVVDFIKTKTTFDSVKIYDANPDI 70

Query: 125 LHAFAGTAISLTVSVANADIPALAKLPAAQSWIASNILPFHPATLINHIAVGNEILATSD 184
           L AFAG+ I++T+ V N +IPA+  +  A+ W+A+N+LPF       ++ VGNEILA++D
Sbjct: 71  LRAFAGSEINITIMVPNGNIPAMVNVANARQWVAANVLPFQQQIKFKYVCVGNEILASND 130

Query: 185 KNLIAHMLPAMKALHTALKLANITDIKVSTPHSLGILSASEPPSAGRFRRGYDRVIFAPI 244
            NLI++++PAM++L+ ALK +N+T IKV+TPH+  I      PS  RF     + IF  I
Sbjct: 131 NNLISNLVPAMQSLNEALKASNLTYIKVTTPHAFTISYNRNTPSESRFTND-QKDIFTKI 190

Query: 245 LEFHRQTQTPFMVNPYPFFGFNSTTLNYALFKPNTGVFDKATGKLYTNMFDAQMDAVYSA 304
           LEFHRQ ++PFM+N Y FF  ++  +NYA+F P+  + D  T + YTNMFDA MDA YSA
Sbjct: 191 LEFHRQAKSPFMINAYTFFTMDTNNVNYAIFGPSNAITDTNTQQTYTNMFDAVMDATYSA 250

Query: 305 MKKLGYGDVDIVVAETGWPSVGEPNQPGVTLENAISFNRNLVKHVSSGMGTPMMPNRTFE 364
           MK LGYGDVDI V ETGWP+  + +    + +NA ++N N++K  +  +GTP+MPNR  +
Sbjct: 251 MKALGYGDVDIAVGETGWPTACDAS--WCSPQNAENYNLNIIKR-AQVIGTPLMPNRHID 310

Query: 365 TYVFSLFNENLKPS-VSERNYGLFNPDFTPVYDVGILRNEPSKGPTAPGGQAP---KPSD 424
            ++F+LFNE+ KP    ERN+G+F PDF+P+YDVG+L+          GG +P    P +
Sbjct: 311 IFIFALFNEDGKPGPTRERNWGIFKPDFSPMYDVGVLK----------GGGSPLPFPPIN 370

Query: 425 AGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQF 484
              KWCV K +A+   LQ NID+VCS G+DC PI  GG C+D N + + +S+ M+AY+Q 
Sbjct: 371 NNGKWCVGKPEATLMQLQANIDWVCSHGIDCTPISPGGICFDNNNMTTRSSFIMNAYYQS 430

Query: 485 AGRHDYNCDFDHTGLLTSTDPSYEACSYPL 511
            G  D  CDF  TG++TST+PS   C  P+
Sbjct: 431 KGCVDVVCDFSGTGIVTSTNPSTSTCPIPI 444

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022948604.16.8e-24286.76glucan endo-1,3-beta-glucosidase-like [Cucurbita moschata][more]
KAG6607245.11.5e-24186.76Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022998328.12.0e-24186.76glucan endo-1,3-beta-glucosidase-like [Cucurbita maxima][more]
XP_023522990.13.7e-24086.15glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo][more]
KAG7036929.15.9e-23883.37Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. argyros... [more]
Match NameE-valueIdentityDescription
P524091.0e-13654.35Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1[more]
Q94G866.2e-11346.20Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Q9M0698.1e-11345.96Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q8VYE51.3e-8940.34Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9FJU94.3e-8237.95Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Match NameE-valueIdentityDescription
A0A6J1GAD33.3e-24286.76(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111452230 P... [more]
A0A6J1KGF29.6e-24286.76(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111492999 PE=... [more]
A0A5D3BDL66.9e-23279.26(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7VQ766.9e-23278.54(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A0A0LVC13.8e-23082.28(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G522000 PE=3... [more]
Match NameE-valueIdentityDescription
AT5G24318.11.2e-13853.11O-Glycosyl hydrolases family 17 protein [more]
AT5G24318.21.4e-13653.15O-Glycosyl hydrolases family 17 protein [more]
AT3G55430.15.5e-13354.05O-Glycosyl hydrolases family 17 protein [more]
AT3G46570.18.6e-12667.16Glycosyl hydrolase superfamily protein [more]
AT2G39640.17.3e-11747.78glycosyl hydrolase family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 85..397
e-value: 4.5E-81
score: 272.9
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 313..326
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 83..405
e-value: 2.0E-116
score: 390.4
NoneNo IPR availablePANTHERPTHR32227:SF359GLUCOSIDASE-LIKE PROTEINcoord: 65..404
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 65..404
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 85..398

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0023995.1Sed0023995.1mRNA
Sed0023995.2Sed0023995.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds