Sed0023991 (gene) Chayote v1

Overview
NameSed0023991
Typegene
OrganismSechium edule (Chayote v1)
Descriptionmechanosensitive ion channel protein 1, mitochondrial-like
LocationLG12: 906125 .. 910701 (+)
RNA-Seq ExpressionSed0023991
SyntenySed0023991
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCGAGCTCACTGTTCACAAGAATGTTGCTTCGATTTTCTTCACCCTTACAGATCCTTTTCCATTTCGTTGCTCGCAAGCAAGGAACTCGTCTGAAGAGCTTCAAGGAGCTGTTGCTTCATTCCGTTTCCTCCATTATAGGTCAGTGATCTTGAGGTTTCTAAAAATATATGTTCTAGTTTCAAGATTAAGTTAGAGGAGAATTTATTGGAATTTACCGGTGATTTGTGTGTTTGATCGTTTGTTTTAGCTAACGATTTGATTCGATTTTTTCCCTTCAATTTATGGTTTTAGATTAGGGTTTTTAGTATATGGATGTAGTCATATGTGTTTTTTTTTCTATGAATTGTTTTTTTGTGATGTTGAATCAATCTGTTCTTGGATTTTCATTAGCTGATTATATTCTCTTGCAGTTTTGGAACTCAAGTGATAAATCTTGAGTTCAGTCTGGTTCTTAGGGTGTTTATTGGATGCGAGCCGAAGCAATGGCTGGCACAAGGCTTTTCATTCTCAAGTCGATTTATAGTTCCTTCAACTTTTATTCGAAGCCGTGCTCGTTTCAGTCTTGCAAGTCTTACATAAAGATTTCCGAGCACGCTGACCGGGTTGTTAGACCAAGTGTTCTAAATTTACAACACTGCAAAGATGAATCTCGCAGCTCGAAGAACATCTCACACATTGGTTTCCAAAGAGCAGCTTCTGTATGTCGTTTACATATGCACAGTTACGGTACATTGTTGCCTGCATCATCAGCGAAATACTGGAACACTATGCCTCTTACTTCTCTCAGCCTTCTGTCAAATCGTCGGTTATATTCATCGGCTTCTAGCGGGAAAGTTGATATAAATGGAGATGCTGGTGTTCCAGCAGCTACTTCTGGTGTTGATGTGGATATTAGCAACCCCTCAGATGTTGGAAGCAATTTGCTTGAAAGAGCTAAGGATGTCTGGCAGGGTGCTTTAGATGCAGCATCTTTTACTGGGCAGAAGGCTAGAGAAGTGTATGATGAGTTGTCTCCTTATGCAGATAAACTATTGGATTCACATCCATATCTAAGAAATGTAATTGTTCCTGTGAGTATGACCTTAACTGCTACTCTTTTGGCTTGGGTGGTAATGCCTAGGCTTTTGAGAAGATTCCATAAGTATTCAATGCGAGGTCCTGTCGCCATACTATCTGGAAGCATATCTGTTGAGGAAATTCCCTATGAGAAAAGTTTTTGGGGTGCCTTGGAGGATCCTTTACGATATCTGGTTACCTTCTTTGCATTCTCTCAAATGTTAGTGTCTCAAACTTCAGCGTATATCTCTTAACTGCACTATGGTTTTTATCTTCCCTTAATATTTGTCTAAACTTTGATTTGCAGTGGCACAATGGTTGCACCAACCGCAGTAGCATCAGAGTTTGTTTCACAGGCATGCAGGGGAGCTGTTATCCTTTCCCTTGTCTGGTTTATATATCGTTGGAAAACAAACGTGTTATCTCGAGCCTTGGTCTCCCAGAATTTTGCAAGGCTTGAGCGGGAAAGGCTAATTACACTAGACAAAGTTTCATCTGTTGGCCTATTTGCAATCGGATTAATGGCTTTGGCCGAGGCATCTGGGGTGGCTGTTCAATCTATGTTGACAGTTGGTGGCATTGGAGGTAATAGCTTGCAGTCAAATGGCTTTGCAGTTTTATTTCCATTCTAATTGCAGCCATTTTTTGGCAATAAACAAGCTTGCTTATTGTTAATGACCTGGAACATGTGTAATGTAATTCAAATCTCTTCCTCGTTGAAGGAGTGGCCACTGCTTTTGCTGCAAGGGACGTACTTGGGAATTTTCTAAGTGGCTTAACAATGCAGTTTTCACAACCCTTCTCTCTCGGGGATACAATCAAGGTGTGTAAGTAATAATTTTCCTTCTTTATCTTTAGGTGAGGGACGGAGATGTGTCTAGTACTCCTGTCGCATATATGAATATGATCCAGATCCACAAAAGTTGCAAGATTTGAATATGAGGTCGAGAGTTCATTTTTTTCTTTTACTACTGTCTTTCTTATTCTGTTGATAATAGCATAGTTGAAGTTGATACCTCAGTGATGCTTGGACTTCTAGATTACATTTATCTTTGTGTCATGTTGATCTTTTTGTTATACATTGTTATTTTATTAAATAGTATGAGGTTCCAACTCAACTCAAAACCAATTGGTAATGAGAGGAGTATCTCTATGAACTTATCAAGCTTGTGTGGCTTCCTCGCCTTTTTAAGAAGGGACAAGGGGTGAGGATCCTCAAATTATATGGTCAAATCCATGGTGGTGAATCACAGTCATGTGTGTAATTTTAGTCTATAATCTCTCTCTGTATTTACTAGAAGGTAATGACATCAAACAAATTGGTGGAAACTTGTAGATTGTTTCTGCAAGTTTGTTTTTATTTGTCCTTTCTAGTTAATTTAAAATCAAACGAGTTGCAATTTTCCATTTGCAGGCTGGTGCTATTGAAGGTCAAGTTGTGGAAATGGGTCTCATGACAACATCATTATTAAGTGCTGAAAAATTTCCAATCGTTGTTCCAAATTCTCTCTTTTCTAGTCAGGTGGATCCTTTTTTTTTCCTCACTTTTATGTACTTGTATTTTTTATTACTTTTATATTATTATCATCTTCCAAATTCTCCATGGCCTTTTATTTATATTCCTATATAATCACTATATCTTTACTTGGTCACCAGCCGTACTGAACTCAATGCTTTTCTTATTTAGAATTTTTTATAATGTCAAGGCATATTTGATTAAGTCTCCACAGCTGAACGATGTATGTGTGTAGCCATTAATTTGATTAAGTACATTTGTTATCAGGTAGATTTGAGGGATATTGACTTTCTATTTGAAATCAGGTTTCGAGTTCTTTTTTTGTCCAATACATTTTTCTGGAAGTCTCTGAGTTTGTCTTGGCAATATCTTTTACGAATTTTGGCATTTTCCAGGTGGATTTATAAATTATCCATTTTAGATATCTCTATAACTTGTGAAAATATCACATTTCTAACTCTAGTGAGGAGAAAAGTTCAGCAAGAAACTTTTTTGGACTTGGATGTATTGAGATACAAATAAAGTTGTCTCAGAAGATTATAGGTATATGAGTGACGACAACCATCTTCGTTGAAATGAAACTTTTTGGATGAAACAAAAACTAAGTCATGAGAGCATATGCCTAAAGTAGAAAATATCATATTAATATGAACATCAGTCCAGGATTGTTGTTCTTATTATAGATGGTCAATAGTCATGACTCAAAAGTGGTGATTGTTCTCGTCTTTCACTCGGAAAACTTTCTTGTAAGGCCATCTCGATTCTTGAATTCCTGCGGGAATATGTTTGGCATAGCTTTGTTGTGTTCTTAATGTTTTCGCATGAATCTCTTTGCTAGATTGGTTAAGGGTGTTTGCTCATTTGGTCGTATGCTTCAACCCACTTCTTGAATTTATGTCTGACTGCGATGGATTTCTTGGATTAAGATAGCAGTTTTGATGAATTTATTTACTGTGTGGTTACATAAAGTTTCTGTTAAAAATGAAATGATAATGTTTGCAGGTAATTATAAATAAGTCTCGAGCGCAATGGCGGGCAATCACGAAGAAAATTCCCTTGCAAGTTGATGATCTTAGTACGGTTTCTCAGATATCGGATTATATTAAGAGTATGCTTAGGTCTCACCCTAAAGTGTTCTTGGGAAAGGAGGCTCCTTATGCTTTCCTCTCCCAAATTGAAAGCACATATGCAGAATTGACAATTGGTTGCAACCTTCAAAAGATGGTAAGTTTGATATTTCCAATGGAAACCTTACCTGTTAATGTCTTACGGTCATGTCGTAGTTGCTCGACGTGTTGTCCCTTCGAACTTCACGCTTACTTCTAAATTGTATTTGTGTAGGGTAAAGAAGAGCTTTTCTCTACTGAGCAAGATATTCTTCTTCAATCAGTGCAAATCATTAAGGAAAATGGCGCAACCCTAGGTACTACCATGTCGGATTGGACAAACAAGTGATGATGCAAATTATACTTACTTCAACCTTAGATTCTGCTACTCGTCGTCTATGCGAGCACTTTGGCGATGTTTCTTGATGTGACTGTAAAGCCACGAATTCCGCTGTCCAGTTTGTTAAACTCGTGTTTTTGAAGAAAACAACTCATCCATCAATGTAAAGGTCTGAAATCCCCACCTTACAAACTTCCTTGATGATGTACAGTTTGTCTTTGTTGTTTTTGGTAGCCTGGAATCTATGTGTGCGAAGAGAATGTTGGAACGGAATGCTCGATATCACTGCTTGTCACAGGCATGGGCACGGGCGTGGGCATTTTTGGATAGTTTCACAGCTCACTCATGCCAAATGTTGTAATAGAAAGGGTTGCCGAAAAAATTCTTTTGAAACCAGTTCTTACCCATGAAGCTTTGCTGAATTTTGTGATTCAAAGTTTAGATTTACTCCCTTTTCAAATTAGTTTAATTAGCATGTTTTAATGGCTATATTTGGTATTGTGTTTTAATTCAAATTGATTTTGAGATTGGGTTTTGGTTTATCTTAAGTAACTTAG

mRNA sequence

GCCGAGCTCACTGTTCACAAGAATGTTGCTTCGATTTTCTTCACCCTTACAGATCCTTTTCCATTTCGTTGCTCGCAAGCAAGGAACTCGTCTGAAGAGCTTCAAGGAGCTGTTGCTTCATTCCGTTTCCTCCATTATAGTTTTGGAACTCAAGTGATAAATCTTGAGTTCAGTCTGGTTCTTAGGGTGTTTATTGGATGCGAGCCGAAGCAATGGCTGGCACAAGGCTTTTCATTCTCAAGTCGATTTATAGTTCCTTCAACTTTTATTCGAAGCCGTGCTCGTTTCAGTCTTGCAAGTCTTACATAAAGATTTCCGAGCACGCTGACCGGGTTGTTAGACCAAGTGTTCTAAATTTACAACACTGCAAAGATGAATCTCGCAGCTCGAAGAACATCTCACACATTGGTTTCCAAAGAGCAGCTTCTGTATGTCGTTTACATATGCACAGTTACGGTACATTGTTGCCTGCATCATCAGCGAAATACTGGAACACTATGCCTCTTACTTCTCTCAGCCTTCTGTCAAATCGTCGGTTATATTCATCGGCTTCTAGCGGGAAAGTTGATATAAATGGAGATGCTGGTGTTCCAGCAGCTACTTCTGGTGTTGATGTGGATATTAGCAACCCCTCAGATGTTGGAAGCAATTTGCTTGAAAGAGCTAAGGATGTCTGGCAGGGTGCTTTAGATGCAGCATCTTTTACTGGGCAGAAGGCTAGAGAAGTGTATGATGAGTTGTCTCCTTATGCAGATAAACTATTGGATTCACATCCATATCTAAGAAATGTAATTGTTCCTGTGAGTATGACCTTAACTGCTACTCTTTTGGCTTGGGTGGTAATGCCTAGGCTTTTGAGAAGATTCCATAAGTATTCAATGCGAGGTCCTGTCGCCATACTATCTGGAAGCATATCTGTTGAGGAAATTCCCTATGAGAAAAGTTTTTGGGGTGCCTTGGAGGATCCTTTACGATATCTGGTTACCTTCTTTGCATTCTCTCAAATTGGCACAATGGTTGCACCAACCGCAGTAGCATCAGAGTTTGTTTCACAGGCATGCAGGGGAGCTGTTATCCTTTCCCTTGTCTGGTTTATATATCGTTGGAAAACAAACGTGTTATCTCGAGCCTTGGTCTCCCAGAATTTTGCAAGGCTTGAGCGGGAAAGGCTAATTACACTAGACAAAGTTTCATCTGTTGGCCTATTTGCAATCGGATTAATGGCTTTGGCCGAGGCATCTGGGGTGGCTGTTCAATCTATGTTGACAGTTGGTGGCATTGGAGGAGTGGCCACTGCTTTTGCTGCAAGGGACGTACTTGGGAATTTTCTAAGTGGCTTAACAATGCAGTTTTCACAACCCTTCTCTCTCGGGGATACAATCAAGGCTGGTGCTATTGAAGGTCAAGTTGTGGAAATGGGTCTCATGACAACATCATTATTAAGTGCTGAAAAATTTCCAATCGTTGTTCCAAATTCTCTCTTTTCTAGTCAGGTAATTATAAATAAGTCTCGAGCGCAATGGCGGGCAATCACGAAGAAAATTCCCTTGCAAGTTGATGATCTTAGTACGGTTTCTCAGATATCGGATTATATTAAGAGTATGCTTAGGTCTCACCCTAAAGTGTTCTTGGGAAAGGAGGCTCCTTATGCTTTCCTCTCCCAAATTGAAAGCACATATGCAGAATTGACAATTGGTTGCAACCTTCAAAAGATGGGTAAAGAAGAGCTTTTCTCTACTGAGCAAGATATTCTTCTTCAATCAGTGCAAATCATTAAGGAAAATGGCGCAACCCTAGGTACTACCATGTCGGATTGGACAAACAAGTGATGATGCAAATTATACTTACTTCAACCTTAGATTCTGCTACTCGTCGTCTATGCGAGCACTTTGGCGATGTTTCTTGATGTGACTGTAAAGCCACGAATTCCGCTGTCCAGTTTGTTAAACTCGTGTTTTTGAAGAAAACAACTCATCCATCAATGTAAAGGTCTGAAATCCCCACCTTACAAACTTCCTTGATGATGTACAGTTTGTCTTTGTTGTTTTTGGTAGCCTGGAATCTATGTGTGCGAAGAGAATGTTGGAACGGAATGCTCGATATCACTGCTTGTCACAGGCATGGGCACGGGCGTGGGCATTTTTGGATAGTTTCACAGCTCACTCATGCCAAATGTTGTAATAGAAAGGGTTGCCGAAAAAATTCTTTTGAAACCAGTTCTTACCCATGAAGCTTTGCTGAATTTTGTGATTCAAAGTTTAGATTTACTCCCTTTTCAAATTAGTTTAATTAGCATGTTTTAATGGCTATATTTGGTATTGTGTTTTAATTCAAATTGATTTTGAGATTGGGTTTTGGTTTATCTTAAGTAACTTAG

Coding sequence (CDS)

ATGCGAGCCGAAGCAATGGCTGGCACAAGGCTTTTCATTCTCAAGTCGATTTATAGTTCCTTCAACTTTTATTCGAAGCCGTGCTCGTTTCAGTCTTGCAAGTCTTACATAAAGATTTCCGAGCACGCTGACCGGGTTGTTAGACCAAGTGTTCTAAATTTACAACACTGCAAAGATGAATCTCGCAGCTCGAAGAACATCTCACACATTGGTTTCCAAAGAGCAGCTTCTGTATGTCGTTTACATATGCACAGTTACGGTACATTGTTGCCTGCATCATCAGCGAAATACTGGAACACTATGCCTCTTACTTCTCTCAGCCTTCTGTCAAATCGTCGGTTATATTCATCGGCTTCTAGCGGGAAAGTTGATATAAATGGAGATGCTGGTGTTCCAGCAGCTACTTCTGGTGTTGATGTGGATATTAGCAACCCCTCAGATGTTGGAAGCAATTTGCTTGAAAGAGCTAAGGATGTCTGGCAGGGTGCTTTAGATGCAGCATCTTTTACTGGGCAGAAGGCTAGAGAAGTGTATGATGAGTTGTCTCCTTATGCAGATAAACTATTGGATTCACATCCATATCTAAGAAATGTAATTGTTCCTGTGAGTATGACCTTAACTGCTACTCTTTTGGCTTGGGTGGTAATGCCTAGGCTTTTGAGAAGATTCCATAAGTATTCAATGCGAGGTCCTGTCGCCATACTATCTGGAAGCATATCTGTTGAGGAAATTCCCTATGAGAAAAGTTTTTGGGGTGCCTTGGAGGATCCTTTACGATATCTGGTTACCTTCTTTGCATTCTCTCAAATTGGCACAATGGTTGCACCAACCGCAGTAGCATCAGAGTTTGTTTCACAGGCATGCAGGGGAGCTGTTATCCTTTCCCTTGTCTGGTTTATATATCGTTGGAAAACAAACGTGTTATCTCGAGCCTTGGTCTCCCAGAATTTTGCAAGGCTTGAGCGGGAAAGGCTAATTACACTAGACAAAGTTTCATCTGTTGGCCTATTTGCAATCGGATTAATGGCTTTGGCCGAGGCATCTGGGGTGGCTGTTCAATCTATGTTGACAGTTGGTGGCATTGGAGGAGTGGCCACTGCTTTTGCTGCAAGGGACGTACTTGGGAATTTTCTAAGTGGCTTAACAATGCAGTTTTCACAACCCTTCTCTCTCGGGGATACAATCAAGGCTGGTGCTATTGAAGGTCAAGTTGTGGAAATGGGTCTCATGACAACATCATTATTAAGTGCTGAAAAATTTCCAATCGTTGTTCCAAATTCTCTCTTTTCTAGTCAGGTAATTATAAATAAGTCTCGAGCGCAATGGCGGGCAATCACGAAGAAAATTCCCTTGCAAGTTGATGATCTTAGTACGGTTTCTCAGATATCGGATTATATTAAGAGTATGCTTAGGTCTCACCCTAAAGTGTTCTTGGGAAAGGAGGCTCCTTATGCTTTCCTCTCCCAAATTGAAAGCACATATGCAGAATTGACAATTGGTTGCAACCTTCAAAAGATGGGTAAAGAAGAGCTTTTCTCTACTGAGCAAGATATTCTTCTTCAATCAGTGCAAATCATTAAGGAAAATGGCGCAACCCTAGGTACTACCATGTCGGATTGGACAAACAAGTGA

Protein sequence

MRAEAMAGTRLFILKSIYSSFNFYSKPCSFQSCKSYIKISEHADRVVRPSVLNLQHCKDESRSSKNISHIGFQRAASVCRLHMHSYGTLLPASSAKYWNTMPLTSLSLLSNRRLYSSASSGKVDINGDAGVPAATSGVDVDISNPSDVGSNLLERAKDVWQGALDAASFTGQKAREVYDELSPYADKLLDSHPYLRNVIVPVSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAILSGSISVEEIPYEKSFWGALEDPLRYLVTFFAFSQIGTMVAPTAVASEFVSQACRGAVILSLVWFIYRWKTNVLSRALVSQNFARLERERLITLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQISDYIKSMLRSHPKVFLGKEAPYAFLSQIESTYAELTIGCNLQKMGKEELFSTEQDILLQSVQIIKENGATLGTTMSDWTNK
Homology
BLAST of Sed0023991 vs. NCBI nr
Match: XP_022969688.1 (mechanosensitive ion channel protein 1, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 831.6 bits (2147), Expect = 3.8e-237
Identity = 438/531 (82.49%), Postives = 467/531 (87.95%), Query Frame = 0

Query: 10  RLFILKSIYSSFNFYSKPCSFQSCKSYIKISEHADRVVRPS-VLNLQHCKDESRSSKNIS 69
           RLFIL SIYS+   Y  PCSFQ CKSY KISE+ +RV+RPS VLN Q+ KDES S KN+S
Sbjct: 2   RLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRPSVVLNKQYYKDESHSLKNVS 61

Query: 70  HIGFQRAASVCRLHMHSYG-----TLLPASSAKYWNTMPLTSLSLLSNRRLYSSASSGKV 129
             GF RA SVCRLH  SYG     T  PAS  KYWNT+P TSL+ LSN R YSS SS K 
Sbjct: 62  RTGFIRAPSVCRLHTPSYGTHPIATFSPASPVKYWNTIPPTSLAYLSNPRFYSSTSSRKA 121

Query: 130 DINGDAGVPAATSGVDVDISNPSDVGSNLLERAKDVWQGALDAASFTGQKAREVYDELSP 189
           DI GD GVP A+SGV+ DISNP DVG + LER KD WQ A+DAASFTGQKAREV DE SP
Sbjct: 122 DITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSAVDAASFTGQKAREVSDEFSP 181

Query: 190 YADKLLDSHPYLRNVIVPVSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAILSGSISVEE 249
           + DK+LDSHPYL+N I PV MTLTATLLAWVVMPRLLRRFHKYSMR PVAILSGS S+EE
Sbjct: 182 HVDKILDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIEE 241

Query: 250 IPYEKSFWGALEDPLRYLVTFFAFSQIGTMVAPTAVASEFVSQACRGAVILSLVWFIYRW 309
           IPYEKSFWGALEDPLRYLVTF+AFSQIG MVAPTA+ASEFVSQACRGAVILSLVWFIYRW
Sbjct: 242 IPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYRW 301

Query: 310 KTNVLSRALVSQNFARLERERLITLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG 369
           KTNV SRAL SQNF+ L+RE+L+TLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG
Sbjct: 302 KTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG 361

Query: 370 VATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIV 429
           VATAFA+RD+LGN LSGLTMQFSQPFSLGDTIKAGAIEGQV+EMGLMTTSLLSAEKFPIV
Sbjct: 362 VATAFASRDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVIEMGLMTTSLLSAEKFPIV 421

Query: 430 VPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQISDYIKSMLRSHPKVFLGKEAP 489
           VPNSLFSSQVIINKSRAQWRAITKKIP QV D STVS+ISD IKSMLRSHPKVFLGKEAP
Sbjct: 422 VPNSLFSSQVIINKSRAQWRAITKKIPFQVADFSTVSKISDDIKSMLRSHPKVFLGKEAP 481

Query: 490 YAFLSQIESTYAELTIGCNLQKMGKEELFSTEQDILLQSVQIIKENGATLG 535
           Y FLSQIES+YAELTIGCNL++MGKEELFSTEQDILLQSVQIIKENGATLG
Sbjct: 482 YCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIIKENGATLG 532

BLAST of Sed0023991 vs. NCBI nr
Match: XP_023538305.1 (mechanosensitive ion channel protein 1, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 831.6 bits (2147), Expect = 3.8e-237
Identity = 438/531 (82.49%), Postives = 466/531 (87.76%), Query Frame = 0

Query: 10  RLFILKSIYSSFNFYSKPCSFQSCKSYIKISEHADRVVRPS-VLNLQHCKDESRSSKNIS 69
           RLFIL SIYS+   Y  PCSFQ CKSY KISE+ +RV+RPS VLN Q+ KDES S KN+S
Sbjct: 2   RLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRPSVVLNKQYYKDESHSLKNVS 61

Query: 70  HIGFQRAASVCRLHMHSYG-----TLLPASSAKYWNTMPLTSLSLLSNRRLYSSASSGKV 129
             GF RA SVCRLH  SYG     T  PAS  KYWNT+P TSL  LSN R YSS SS K 
Sbjct: 62  RTGFLRAPSVCRLHTPSYGTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRKA 121

Query: 130 DINGDAGVPAATSGVDVDISNPSDVGSNLLERAKDVWQGALDAASFTGQKAREVYDELSP 189
           DI GD GVP A+SGV+ DISNP DVG + LER KD WQ A+DAASFTGQKAREV DE SP
Sbjct: 122 DITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSAVDAASFTGQKAREVSDEFSP 181

Query: 190 YADKLLDSHPYLRNVIVPVSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAILSGSISVEE 249
           + DK+LDSHPYL+N I PV MTLTATLLAWVVMPRLLRRFHKYSMR PVAILSGS S+EE
Sbjct: 182 HVDKILDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIEE 241

Query: 250 IPYEKSFWGALEDPLRYLVTFFAFSQIGTMVAPTAVASEFVSQACRGAVILSLVWFIYRW 309
           IPYEKSFWGALEDPLRYLVTF+AFSQIG MVAPTA+ASEFVSQACRGAVILSLVWFIYRW
Sbjct: 242 IPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYRW 301

Query: 310 KTNVLSRALVSQNFARLERERLITLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG 369
           KTNV SRAL SQNF+ L+RE+L+TLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG
Sbjct: 302 KTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG 361

Query: 370 VATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIV 429
           VATAFAARD+LGN LSGLTMQFSQPFSLGDTIKAGAIEGQV+EMGLMTTSLLSAEKFPIV
Sbjct: 362 VATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVIEMGLMTTSLLSAEKFPIV 421

Query: 430 VPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQISDYIKSMLRSHPKVFLGKEAP 489
           VPNSLFSSQVIINKSRAQWRAITKKIP QV D STVS+ISD IKSMLRSHPKVFLGKEAP
Sbjct: 422 VPNSLFSSQVIINKSRAQWRAITKKIPFQVADFSTVSKISDDIKSMLRSHPKVFLGKEAP 481

Query: 490 YAFLSQIESTYAELTIGCNLQKMGKEELFSTEQDILLQSVQIIKENGATLG 535
           Y FLSQIES+YAELTIGCNL++MGKEELFSTEQDILLQSVQI+KENGATLG
Sbjct: 482 YCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIMKENGATLG 532

BLAST of Sed0023991 vs. NCBI nr
Match: KAG6586069.1 (Mechanosensitive ion channel protein 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 826.6 bits (2134), Expect = 1.2e-235
Identity = 436/531 (82.11%), Postives = 464/531 (87.38%), Query Frame = 0

Query: 10  RLFILKSIYSSFNFYSKPCSFQSCKSYIKISEHADRVVRPS-VLNLQHCKDESRSSKNIS 69
           RLFIL SIYS+   Y  PCSFQ CKSY KISE+ +RV+RPS VLN Q+ KDES S KN+S
Sbjct: 2   RLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRPSVVLNKQYYKDESHSLKNVS 61

Query: 70  HIGFQRAASVCRLHMHSY-----GTLLPASSAKYWNTMPLTSLSLLSNRRLYSSASSGKV 129
             GF RA SVC LH  SY     GT  PAS  KYWNT+P TSL  LSN R YSS SS K 
Sbjct: 62  RTGFLRAPSVCCLHTPSYGTHPAGTFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRKA 121

Query: 130 DINGDAGVPAATSGVDVDISNPSDVGSNLLERAKDVWQGALDAASFTGQKAREVYDELSP 189
           DI GD GVP A+SGV+ DISNP DVG + LER KD WQ  +DAASFTGQKAREV DE SP
Sbjct: 122 DITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSVVDAASFTGQKAREVSDEFSP 181

Query: 190 YADKLLDSHPYLRNVIVPVSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAILSGSISVEE 249
           + DKLLDSHPYL+N I PV MTLTATLLAWVVMPRLLRRFHKYSMR PVAILSGS S+EE
Sbjct: 182 HVDKLLDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIEE 241

Query: 250 IPYEKSFWGALEDPLRYLVTFFAFSQIGTMVAPTAVASEFVSQACRGAVILSLVWFIYRW 309
           IPYEKSFWGALEDPLRYLVTF+AFSQIG MVAPTA+ASEFVSQACRGAVILSLVWFIYRW
Sbjct: 242 IPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYRW 301

Query: 310 KTNVLSRALVSQNFARLERERLITLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG 369
           KTNV SRAL SQNF+ L+RE+L+TLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG
Sbjct: 302 KTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG 361

Query: 370 VATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIV 429
           VATAFAARD+LGN LSGLTMQFSQPFSLGDTIKAGAIEGQV+EMGLMTTSLLSAEKFPIV
Sbjct: 362 VATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVIEMGLMTTSLLSAEKFPIV 421

Query: 430 VPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQISDYIKSMLRSHPKVFLGKEAP 489
           VPNSLFSSQVIINKSRAQWRAITKKIP QV DLSTVS+ISD IKSML+SHPKVFLGKEAP
Sbjct: 422 VPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKSMLKSHPKVFLGKEAP 481

Query: 490 YAFLSQIESTYAELTIGCNLQKMGKEELFSTEQDILLQSVQIIKENGATLG 535
           Y FLSQIES+YAELTIGCNL++MGKEELFSTEQDILLQSVQI+KENGAT G
Sbjct: 482 YCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIMKENGATFG 532

BLAST of Sed0023991 vs. NCBI nr
Match: XP_022937845.1 (mechanosensitive ion channel protein 1, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 825.9 bits (2132), Expect = 2.1e-235
Identity = 438/531 (82.49%), Postives = 464/531 (87.38%), Query Frame = 0

Query: 10  RLFILKSIYSSFNFYSKPCSFQSCKSYIKISEHADRVVRPS-VLNLQHCKDESRSSKNIS 69
           RLFIL SIYS+   Y  PCSFQ CKSY KISE+ +RV+R S VLN Q+ KDES S KN+S
Sbjct: 2   RLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRLSVVLNKQYYKDESHSLKNVS 61

Query: 70  HIGFQRAASVCRLHMHSYG-----TLLPASSAKYWNTMPLTSLSLLSNRRLYSSASSGKV 129
             GF RA SVC LH  SYG     T  PAS  KYWNT+P TSL  LSN R YSS SS K 
Sbjct: 62  RTGFLRAPSVCCLHTPSYGTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRKA 121

Query: 130 DINGDAGVPAATSGVDVDISNPSDVGSNLLERAKDVWQGALDAASFTGQKAREVYDELSP 189
           DI GD GVP A+SGV+ DISNP DVG + LER KD WQ  +DAASFTGQKAREV DE SP
Sbjct: 122 DITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSVVDAASFTGQKAREVSDEFSP 181

Query: 190 YADKLLDSHPYLRNVIVPVSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAILSGSISVEE 249
           + DKLLDSHPYL+N I PV MTLTATLLAWVVMPRLLRRFHKYSMR PVAILSGS S+EE
Sbjct: 182 HVDKLLDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIEE 241

Query: 250 IPYEKSFWGALEDPLRYLVTFFAFSQIGTMVAPTAVASEFVSQACRGAVILSLVWFIYRW 309
           IPYEKSFWGALEDPLRYLVTF+AFSQIG MVAPTA+ASEFVSQACRGAVILSLVWFIYRW
Sbjct: 242 IPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYRW 301

Query: 310 KTNVLSRALVSQNFARLERERLITLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG 369
           KTNV SRAL SQNF+ L+RE+L+TLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG
Sbjct: 302 KTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG 361

Query: 370 VATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIV 429
           VATAFAARD+LGN LSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIV
Sbjct: 362 VATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIV 421

Query: 430 VPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQISDYIKSMLRSHPKVFLGKEAP 489
           VPNSLFSSQVIINKSRAQWRAITKKIP QV DLSTVS+ISD IKSMLRSHPKVFLGKEAP
Sbjct: 422 VPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKSMLRSHPKVFLGKEAP 481

Query: 490 YAFLSQIESTYAELTIGCNLQKMGKEELFSTEQDILLQSVQIIKENGATLG 535
           Y FLSQIES+YAELTIGCNL++MGKEELFSTEQDILLQSVQI+KENGATLG
Sbjct: 482 YCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIMKENGATLG 532

BLAST of Sed0023991 vs. NCBI nr
Match: XP_022147379.1 (mechanosensitive ion channel protein 1, mitochondrial-like [Momordica charantia] >XP_022147381.1 mechanosensitive ion channel protein 1, mitochondrial-like [Momordica charantia] >XP_022147382.1 mechanosensitive ion channel protein 1, mitochondrial-like [Momordica charantia])

HSP 1 Score: 825.9 bits (2132), Expect = 2.1e-235
Identity = 442/546 (80.95%), Postives = 479/546 (87.73%), Query Frame = 0

Query: 6   MAGTRLFILKSIYSSFNFYSKPCSFQSCKSYIKISEHADRVVRPS-VLNLQHCKDESRSS 65
           MAG R+FILKSIY+S   Y  P S QS K Y KISE+ DR +RPS VLN +   D SR +
Sbjct: 1   MAGIRIFILKSIYTSSRSYLNPYSSQSYKPYTKISEYVDRGLRPSTVLNKKCHNDNSRRT 60

Query: 66  KNISHIGFQRAASVCRLHMHSYG-----TLLPASSAKYWNTMPLTSLSLLSNRRLYSSAS 125
           KN S  GF RAASVC L++ S+G     T LPA S +YW T+PLTS   LSN RLYSSAS
Sbjct: 61  KNTSGTGFPRAASVCGLNISSHGAYPIDTFLPAWSVRYWRTVPLTSPGHLSNPRLYSSAS 120

Query: 126 SGKVDINGDAGVPAATSGVDVDISNPSDVGS--NLLERAKDVWQGALDAASFTGQKAREV 185
           S K DI GD GV AA++GV+ DISN  DVG+    LER KDVWQ A+DAASFT QKA+EV
Sbjct: 121 SRKADITGDTGVSAASAGVEADISNTQDVGTGKEWLERVKDVWQSAVDAASFTEQKAKEV 180

Query: 186 YDELSPYADKLLDSHPYLRNVIVPVSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAILSG 245
            DE +P+ DKL DSHPYL+NVIVPVSMTLTATLLAW+VMPRLLRRFHKYSMRGPVA+LSG
Sbjct: 181 SDEFTPHVDKLFDSHPYLKNVIVPVSMTLTATLLAWLVMPRLLRRFHKYSMRGPVALLSG 240

Query: 246 SISVEEIPYEKSFWGALEDPLRYLVTFFAFSQIGTMVAPTAVASEFVSQACRGAVILSLV 305
           SISVEEIPYEKSFWGALEDPLRYLVTFFAFSQIG MVAPT VASE+V+QACRGAVILSLV
Sbjct: 241 SISVEEIPYEKSFWGALEDPLRYLVTFFAFSQIGMMVAPTTVASEYVTQACRGAVILSLV 300

Query: 306 WFIYRWKTNVLSRALVSQNFARLERERLITLDKVSSVGLFAIGLMALAEASGVAVQSMLT 365
           WFIYRWKTNVLSRAL SQ+F+ L+RE+L+TLDKVSSVGLFAIGLMALAEASGVAVQSMLT
Sbjct: 301 WFIYRWKTNVLSRALASQSFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLT 360

Query: 366 VGGIGGVATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSA 425
           VGGIGGVA AFAARD+LGN LSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSA
Sbjct: 361 VGGIGGVAAAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSA 420

Query: 426 EKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQISDYIKSMLRSHPKVF 485
           EKFPIVVPNSLFSSQVI+NKSRAQWRAITKKIPLQVD+LSTVSQISD IKSMLRSHPKVF
Sbjct: 421 EKFPIVVPNSLFSSQVIVNKSRAQWRAITKKIPLQVDNLSTVSQISDNIKSMLRSHPKVF 480

Query: 486 LGKEAPYAFLSQIESTYAELTIGCNLQKMGKEELFSTEQDILLQSVQIIKENGATLGTTM 544
           LGKEAPY FLSQIESTYAELTIGCNL+KM K+ELFSTEQDILLQSVQIIKE+GATLG+TM
Sbjct: 481 LGKEAPYCFLSQIESTYAELTIGCNLRKMSKDELFSTEQDILLQSVQIIKEHGATLGSTM 540

BLAST of Sed0023991 vs. ExPASy Swiss-Prot
Match: Q8VZL4 (Mechanosensitive ion channel protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MSL1 PE=1 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 2.8e-150
Identity = 270/428 (63.08%), Postives = 347/428 (81.07%), Query Frame = 0

Query: 115 YSSASSGKVDINGDAGVPAATSGVDVDISNPSDVGSNLLERAKDVWQGALDAASFTGQKA 174
           Y+ A S K D  G       T   D      +  G++ +E+AKDV Q ++DA + T +K 
Sbjct: 75  YARAFSSKSDDFGSIVASGVTGSGD-----GNGNGNDWVEKAKDVLQTSVDAVTETAKKT 134

Query: 175 REVYDELSPYADKLLDSHPYLRNVIVPVSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAI 234
           ++V DE+ P+  + LDS+PYL++VIVPVS+T+T TL AWVVMPR+LRRFH Y+M+    +
Sbjct: 135 KDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTMTGTLFAWVVMPRILRRFHTYAMQSSAKL 194

Query: 235 LSGSISVEEIPYEKSFWGALEDPLRYLVTFFAFSQIGTMVAPTAVASEFVSQACRGAVIL 294
           L    S E++PYEKSFWGALEDP RYLVTF AF+QI  MVAPT +A+++ S   +GAVIL
Sbjct: 195 LPVGFSNEDVPYEKSFWGALEDPARYLVTFIAFAQIAAMVAPTTIAAQYFSPTVKGAVIL 254

Query: 295 SLVWFIYRWKTNVLSRALVSQNFARLERERLITLDKVSSVGLFAIGLMALAEASGVAVQS 354
           SLVWF+YRWKTNV++R L +++F  L+RE+++TLDKVSSVGLFAIGLMA AEA GVAVQS
Sbjct: 255 SLVWFLYRWKTNVITRMLSAKSFGGLDREKVLTLDKVSSVGLFAIGLMASAEACGVAVQS 314

Query: 355 MLTVGGIGGVATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSL 414
           +LTVGG+GGVATAFAARD+LGN LSGL+MQFS+PFS+GDTIKAG++EGQV+EMGL TTSL
Sbjct: 315 ILTVGGVGGVATAFAARDILGNVLSGLSMQFSRPFSMGDTIKAGSVEGQVIEMGLTTTSL 374

Query: 415 LSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQISDYIKSMLRSHP 474
           L+AEKFP++VPNSLFSSQVI+NKSRAQWRAI  KIPLQ+DDL  + QIS+ IK MLRS+ 
Sbjct: 375 LNAEKFPVLVPNSLFSSQVIVNKSRAQWRAIASKIPLQIDDLDMIPQISNEIKEMLRSNT 434

Query: 475 KVFLGKEAPYAFLSQIESTYAELTIGCNLQKMGKEELFSTEQDILLQSVQIIKENGATLG 534
           KVFLGKEAP+ +LS++E ++AELTIGCNL +MGKEEL++T+Q++LL++V+IIK++G +LG
Sbjct: 435 KVFLGKEAPHCYLSRVEKSFAELTIGCNLIRMGKEELYNTQQEVLLEAVKIIKKHGVSLG 494

Query: 535 TTMSDWTN 543
           TT   W N
Sbjct: 495 TT---WDN 494

BLAST of Sed0023991 vs. ExPASy Swiss-Prot
Match: P0AEB6 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=83334 GN=ynaI PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 4.1e-24
Identity = 81/300 (27.00%), Postives = 151/300 (50.33%), Query Frame = 0

Query: 249 SFW---------GALEDPLRYLVTFFAFSQIGTMV--APTAVASEFVSQACRGAVILSLV 308
           SFW         G L   +++L+     S +G+++         +F++      +  SL+
Sbjct: 27  SFWFRRGNRKRKGFLFHAVQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLI 86

Query: 309 WFIYRWKTNVLSRALVSQNFARLERERLIT----LDKVSSVGLFAIGLMALAEASGVAVQ 368
             I   K  +L      Q   ++++ R IT    + ++  + +  + ++   E  G+++ 
Sbjct: 87  AVILTIKLFLLINQFEKQ---QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLS 146

Query: 369 SMLTVGGIGGVATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKA--GAIEGQVVEMGLMT 428
            +LT GGIGG+A   A +D+L NF SG+ + F +PFS+GD I++    IEG V E+G   
Sbjct: 147 GLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRI 206

Query: 429 TSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQISDYIKSMLR 488
           T + + +  P+ VPNSLFSS  + N  R   R IT  I L+ +D + V  I + ++ ML+
Sbjct: 207 TKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLK 266

Query: 489 SHPKVFLGKEAPYAFLSQIESTYAELTIGCNLQKMGKEELFSTEQDILLQSVQIIKENGA 532
           +HP +   ++    + +Q   +   + + C  +     E  + +QD+ L+ + I++ +GA
Sbjct: 267 NHPAID-QRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGA 322

BLAST of Sed0023991 vs. ExPASy Swiss-Prot
Match: P0AEB5 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) OX=83333 GN=ynaI PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 4.1e-24
Identity = 81/300 (27.00%), Postives = 151/300 (50.33%), Query Frame = 0

Query: 249 SFW---------GALEDPLRYLVTFFAFSQIGTMV--APTAVASEFVSQACRGAVILSLV 308
           SFW         G L   +++L+     S +G+++         +F++      +  SL+
Sbjct: 27  SFWFRRGNRKRKGFLFHAVQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLI 86

Query: 309 WFIYRWKTNVLSRALVSQNFARLERERLIT----LDKVSSVGLFAIGLMALAEASGVAVQ 368
             I   K  +L      Q   ++++ R IT    + ++  + +  + ++   E  G+++ 
Sbjct: 87  AVILTIKLFLLINQFEKQ---QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLS 146

Query: 369 SMLTVGGIGGVATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKA--GAIEGQVVEMGLMT 428
            +LT GGIGG+A   A +D+L NF SG+ + F +PFS+GD I++    IEG V E+G   
Sbjct: 147 GLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRI 206

Query: 429 TSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQISDYIKSMLR 488
           T + + +  P+ VPNSLFSS  + N  R   R IT  I L+ +D + V  I + ++ ML+
Sbjct: 207 TKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLK 266

Query: 489 SHPKVFLGKEAPYAFLSQIESTYAELTIGCNLQKMGKEELFSTEQDILLQSVQIIKENGA 532
           +HP +   ++    + +Q   +   + + C  +     E  + +QD+ L+ + I++ +GA
Sbjct: 267 NHPAID-QRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGA 322

BLAST of Sed0023991 vs. ExPASy Swiss-Prot
Match: O25170 (Uncharacterized MscS family protein HP_0415 OS=Helicobacter pylori (strain ATCC 700392 / 26695) OX=85962 GN=HP_0415 PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 3.8e-14
Identity = 62/203 (30.54%), Postives = 106/203 (52.22%), Query Frame = 0

Query: 290 GAV-ILSLVWFI---YRWKTNVLSRALVSQNFARLERERLITLDKVSSVGLFAIGLMALA 349
           GAV I+ L W +   ++     L   + +++     +E +  + KV    +F + L+ + 
Sbjct: 323 GAVYIMLLAWLVIALFKGYGEALVTNMATKSTHNFRKEVINLILKVVYFLIFIVALLGVL 382

Query: 350 EASGVAVQSMLTVGGIGGVATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKAGAIEGQVV 409
           +  G  V +++   GIGG+A A A +DVL NF + + +     FS GD I  G +EG VV
Sbjct: 383 KQLGFNVSAIIASLGIGGLAVALAVKDVLANFFASVILLLDNSFSQGDWIVCGEVEGTVV 442

Query: 410 EMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQ---- 469
           EMGL  T++ + +   + VPNS  + + I N SR   R + ++I +++    + SQ    
Sbjct: 443 EMGLRRTTIRAFDNALLSVPNSELAGKPIRNWSR---RKVGRRIKMEIGLTYSSSQSALQ 502

Query: 470 --ISDYIKSMLRSHPKVFLGKEA 483
             + D IK ML +HPK+  G ++
Sbjct: 503 LCVKD-IKEMLENHPKIANGADS 521

BLAST of Sed0023991 vs. ExPASy Swiss-Prot
Match: Q9ZKG7 (Uncharacterized MscS family protein jhp_0969 OS=Helicobacter pylori (strain J99 / ATCC 700824) OX=85963 GN=jhp_0969 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 5.0e-14
Identity = 62/203 (30.54%), Postives = 106/203 (52.22%), Query Frame = 0

Query: 290 GAV-ILSLVWFI---YRWKTNVLSRALVSQNFARLERERLITLDKVSSVGLFAIGLMALA 349
           GAV I+ L W +   ++     L   + +++     +E +  + KV    +F + L+ + 
Sbjct: 323 GAVYIMLLAWLVIALFKGYGEALVTNVATKSTHNFRKEVINLILKVVYFLIFIVALLGVL 382

Query: 350 EASGVAVQSMLTVGGIGGVATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKAGAIEGQVV 409
           +  G  V +++   GIGG+A A A +DVL NF + + +     FS GD I  G +EG VV
Sbjct: 383 KQLGFNVSAIIASLGIGGLAVALAVKDVLANFFASVILLLDNSFSQGDWIVCGEVEGTVV 442

Query: 410 EMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQ---- 469
           EMGL  T++ + +   + VPNS  + + I N SR   R + ++I +++    + SQ    
Sbjct: 443 EMGLRRTTIRAFDNALLSVPNSELAGKPIRNWSR---RKVGRRIKMEIGLTYSSSQSALQ 502

Query: 470 --ISDYIKSMLRSHPKVFLGKEA 483
             + D IK ML +HPK+  G ++
Sbjct: 503 LCVKD-IKEMLENHPKIANGADS 521

BLAST of Sed0023991 vs. ExPASy TrEMBL
Match: A0A6J1I1Q4 (mechanosensitive ion channel protein 1, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111468640 PE=3 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.8e-237
Identity = 438/531 (82.49%), Postives = 467/531 (87.95%), Query Frame = 0

Query: 10  RLFILKSIYSSFNFYSKPCSFQSCKSYIKISEHADRVVRPS-VLNLQHCKDESRSSKNIS 69
           RLFIL SIYS+   Y  PCSFQ CKSY KISE+ +RV+RPS VLN Q+ KDES S KN+S
Sbjct: 2   RLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRPSVVLNKQYYKDESHSLKNVS 61

Query: 70  HIGFQRAASVCRLHMHSYG-----TLLPASSAKYWNTMPLTSLSLLSNRRLYSSASSGKV 129
             GF RA SVCRLH  SYG     T  PAS  KYWNT+P TSL+ LSN R YSS SS K 
Sbjct: 62  RTGFIRAPSVCRLHTPSYGTHPIATFSPASPVKYWNTIPPTSLAYLSNPRFYSSTSSRKA 121

Query: 130 DINGDAGVPAATSGVDVDISNPSDVGSNLLERAKDVWQGALDAASFTGQKAREVYDELSP 189
           DI GD GVP A+SGV+ DISNP DVG + LER KD WQ A+DAASFTGQKAREV DE SP
Sbjct: 122 DITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSAVDAASFTGQKAREVSDEFSP 181

Query: 190 YADKLLDSHPYLRNVIVPVSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAILSGSISVEE 249
           + DK+LDSHPYL+N I PV MTLTATLLAWVVMPRLLRRFHKYSMR PVAILSGS S+EE
Sbjct: 182 HVDKILDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIEE 241

Query: 250 IPYEKSFWGALEDPLRYLVTFFAFSQIGTMVAPTAVASEFVSQACRGAVILSLVWFIYRW 309
           IPYEKSFWGALEDPLRYLVTF+AFSQIG MVAPTA+ASEFVSQACRGAVILSLVWFIYRW
Sbjct: 242 IPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYRW 301

Query: 310 KTNVLSRALVSQNFARLERERLITLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG 369
           KTNV SRAL SQNF+ L+RE+L+TLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG
Sbjct: 302 KTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG 361

Query: 370 VATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIV 429
           VATAFA+RD+LGN LSGLTMQFSQPFSLGDTIKAGAIEGQV+EMGLMTTSLLSAEKFPIV
Sbjct: 362 VATAFASRDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVIEMGLMTTSLLSAEKFPIV 421

Query: 430 VPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQISDYIKSMLRSHPKVFLGKEAP 489
           VPNSLFSSQVIINKSRAQWRAITKKIP QV D STVS+ISD IKSMLRSHPKVFLGKEAP
Sbjct: 422 VPNSLFSSQVIINKSRAQWRAITKKIPFQVADFSTVSKISDDIKSMLRSHPKVFLGKEAP 481

Query: 490 YAFLSQIESTYAELTIGCNLQKMGKEELFSTEQDILLQSVQIIKENGATLG 535
           Y FLSQIES+YAELTIGCNL++MGKEELFSTEQDILLQSVQIIKENGATLG
Sbjct: 482 YCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIIKENGATLG 532

BLAST of Sed0023991 vs. ExPASy TrEMBL
Match: A0A6J1D280 (mechanosensitive ion channel protein 1, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111016329 PE=3 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 1.0e-235
Identity = 442/546 (80.95%), Postives = 479/546 (87.73%), Query Frame = 0

Query: 6   MAGTRLFILKSIYSSFNFYSKPCSFQSCKSYIKISEHADRVVRPS-VLNLQHCKDESRSS 65
           MAG R+FILKSIY+S   Y  P S QS K Y KISE+ DR +RPS VLN +   D SR +
Sbjct: 1   MAGIRIFILKSIYTSSRSYLNPYSSQSYKPYTKISEYVDRGLRPSTVLNKKCHNDNSRRT 60

Query: 66  KNISHIGFQRAASVCRLHMHSYG-----TLLPASSAKYWNTMPLTSLSLLSNRRLYSSAS 125
           KN S  GF RAASVC L++ S+G     T LPA S +YW T+PLTS   LSN RLYSSAS
Sbjct: 61  KNTSGTGFPRAASVCGLNISSHGAYPIDTFLPAWSVRYWRTVPLTSPGHLSNPRLYSSAS 120

Query: 126 SGKVDINGDAGVPAATSGVDVDISNPSDVGS--NLLERAKDVWQGALDAASFTGQKAREV 185
           S K DI GD GV AA++GV+ DISN  DVG+    LER KDVWQ A+DAASFT QKA+EV
Sbjct: 121 SRKADITGDTGVSAASAGVEADISNTQDVGTGKEWLERVKDVWQSAVDAASFTEQKAKEV 180

Query: 186 YDELSPYADKLLDSHPYLRNVIVPVSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAILSG 245
            DE +P+ DKL DSHPYL+NVIVPVSMTLTATLLAW+VMPRLLRRFHKYSMRGPVA+LSG
Sbjct: 181 SDEFTPHVDKLFDSHPYLKNVIVPVSMTLTATLLAWLVMPRLLRRFHKYSMRGPVALLSG 240

Query: 246 SISVEEIPYEKSFWGALEDPLRYLVTFFAFSQIGTMVAPTAVASEFVSQACRGAVILSLV 305
           SISVEEIPYEKSFWGALEDPLRYLVTFFAFSQIG MVAPT VASE+V+QACRGAVILSLV
Sbjct: 241 SISVEEIPYEKSFWGALEDPLRYLVTFFAFSQIGMMVAPTTVASEYVTQACRGAVILSLV 300

Query: 306 WFIYRWKTNVLSRALVSQNFARLERERLITLDKVSSVGLFAIGLMALAEASGVAVQSMLT 365
           WFIYRWKTNVLSRAL SQ+F+ L+RE+L+TLDKVSSVGLFAIGLMALAEASGVAVQSMLT
Sbjct: 301 WFIYRWKTNVLSRALASQSFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLT 360

Query: 366 VGGIGGVATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSA 425
           VGGIGGVA AFAARD+LGN LSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSA
Sbjct: 361 VGGIGGVAAAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSA 420

Query: 426 EKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQISDYIKSMLRSHPKVF 485
           EKFPIVVPNSLFSSQVI+NKSRAQWRAITKKIPLQVD+LSTVSQISD IKSMLRSHPKVF
Sbjct: 421 EKFPIVVPNSLFSSQVIVNKSRAQWRAITKKIPLQVDNLSTVSQISDNIKSMLRSHPKVF 480

Query: 486 LGKEAPYAFLSQIESTYAELTIGCNLQKMGKEELFSTEQDILLQSVQIIKENGATLGTTM 544
           LGKEAPY FLSQIESTYAELTIGCNL+KM K+ELFSTEQDILLQSVQIIKE+GATLG+TM
Sbjct: 481 LGKEAPYCFLSQIESTYAELTIGCNLRKMSKDELFSTEQDILLQSVQIIKEHGATLGSTM 540

BLAST of Sed0023991 vs. ExPASy TrEMBL
Match: A0A6J1FHY5 (mechanosensitive ion channel protein 1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111444110 PE=3 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 1.0e-235
Identity = 438/531 (82.49%), Postives = 464/531 (87.38%), Query Frame = 0

Query: 10  RLFILKSIYSSFNFYSKPCSFQSCKSYIKISEHADRVVRPS-VLNLQHCKDESRSSKNIS 69
           RLFIL SIYS+   Y  PCSFQ CKSY KISE+ +RV+R S VLN Q+ KDES S KN+S
Sbjct: 2   RLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRLSVVLNKQYYKDESHSLKNVS 61

Query: 70  HIGFQRAASVCRLHMHSYG-----TLLPASSAKYWNTMPLTSLSLLSNRRLYSSASSGKV 129
             GF RA SVC LH  SYG     T  PAS  KYWNT+P TSL  LSN R YSS SS K 
Sbjct: 62  RTGFLRAPSVCCLHTPSYGTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRKA 121

Query: 130 DINGDAGVPAATSGVDVDISNPSDVGSNLLERAKDVWQGALDAASFTGQKAREVYDELSP 189
           DI GD GVP A+SGV+ DISNP DVG + LER KD WQ  +DAASFTGQKAREV DE SP
Sbjct: 122 DITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSVVDAASFTGQKAREVSDEFSP 181

Query: 190 YADKLLDSHPYLRNVIVPVSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAILSGSISVEE 249
           + DKLLDSHPYL+N I PV MTLTATLLAWVVMPRLLRRFHKYSMR PVAILSGS S+EE
Sbjct: 182 HVDKLLDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIEE 241

Query: 250 IPYEKSFWGALEDPLRYLVTFFAFSQIGTMVAPTAVASEFVSQACRGAVILSLVWFIYRW 309
           IPYEKSFWGALEDPLRYLVTF+AFSQIG MVAPTA+ASEFVSQACRGAVILSLVWFIYRW
Sbjct: 242 IPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYRW 301

Query: 310 KTNVLSRALVSQNFARLERERLITLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG 369
           KTNV SRAL SQNF+ L+RE+L+TLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG
Sbjct: 302 KTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGG 361

Query: 370 VATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIV 429
           VATAFAARD+LGN LSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIV
Sbjct: 362 VATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIV 421

Query: 430 VPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQISDYIKSMLRSHPKVFLGKEAP 489
           VPNSLFSSQVIINKSRAQWRAITKKIP QV DLSTVS+ISD IKSMLRSHPKVFLGKEAP
Sbjct: 422 VPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKSMLRSHPKVFLGKEAP 481

Query: 490 YAFLSQIESTYAELTIGCNLQKMGKEELFSTEQDILLQSVQIIKENGATLG 535
           Y FLSQIES+YAELTIGCNL++MGKEELFSTEQDILLQSVQI+KENGATLG
Sbjct: 482 YCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIMKENGATLG 532

BLAST of Sed0023991 vs. ExPASy TrEMBL
Match: A0A0A0L685 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G121740 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 7.3e-226
Identity = 420/522 (80.46%), Postives = 458/522 (87.74%), Query Frame = 0

Query: 28  CSFQSCKSYIKISEHADRVVRPS-VLNLQHCKDESRSSKNISHIGFQRAASVCRLHMHSY 87
           CSFQS K Y KISE+ DRV+RP+ VLN ++ KDES S K          A+V  LH+ SY
Sbjct: 9   CSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKR---------ATVRGLHIPSY 68

Query: 88  G-----TLLPASSAKYWNTMPLTSLSLLSNRRLYSSASSGKVDINGDAGVPAATSGVDVD 147
           G     T LPASS KYWNT+PLT    LSN R YSSAS  K DI+GD GVPAA+SGV+ D
Sbjct: 69  GTHRIDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPD 128

Query: 148 ISNPSDVGSNLLERAKDVWQGALDAASFTGQKAREVYDELSPYADKLLDSHPYLRNVIVP 207
           ISNP DVG +LLE+ KDVWQ A+DAASFTGQKA+EV DELSP+ DKLLDSHPYL+NVIVP
Sbjct: 129 ISNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVP 188

Query: 208 VSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAILSGSISVEEIPYEKSFWGALEDPLRYL 267
           VSMTL ATLLAW+VMPRLL+RFHKYSMR PVAI+SGS+  EEIPYEKSFWGALEDPLRYL
Sbjct: 189 VSMTLGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYL 248

Query: 268 VTFFAFSQIGTMVAPTAVASEFVSQACRGAVILSLVWFIYRWKTNVLSRALVSQNFARLE 327
           VTFFAFSQIG +VAPTAVA EFVSQACRGAVILSLVWFIYRWKTNVLSRAL ++ FA L+
Sbjct: 249 VTFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLD 308

Query: 328 RERLITLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGNFLSGL 387
           R+RL+TLDKVSSV LFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARD+LGN LSGL
Sbjct: 309 RDRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGL 368

Query: 388 TMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQ 447
           TMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFP+VVPNSLFSSQVIINKSRAQ
Sbjct: 369 TMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQ 428

Query: 448 WRAITKKIPLQVDDLSTVSQISDYIKSMLRSHPKVFLGKEAPYAFLSQIESTYAELTIGC 507
           WRAI K++PLQVDD+STVSQI+D IK+ML SHPKVFLGKEAPY FLSQIESTYAELT+GC
Sbjct: 429 WRAIVKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGC 488

Query: 508 NLQKMGKEELFSTEQDILLQSVQIIKENGATLGTTMSDWTNK 544
           NL+KMGK+E FSTEQDILLQSVQIIK  GATLG+TMSDWTNK
Sbjct: 489 NLRKMGKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTNK 521

BLAST of Sed0023991 vs. ExPASy TrEMBL
Match: A0A1S3AVZ3 (mechanosensitive ion channel protein 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103483257 PE=3 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 3.1e-224
Identity = 415/522 (79.50%), Postives = 452/522 (86.59%), Query Frame = 0

Query: 28  CSFQSCKSYIKISEHADRVVRPS-VLNLQHCKDESRSSKNISHIGFQRAASVCRLHMHSY 87
           CSFQS K Y KIS++ DRV+RP+ VLN ++  DES S K          A+VC LH+ SY
Sbjct: 9   CSFQSFKPYTKISKYVDRVLRPNVVLNKRYYNDESHSFKR---------ATVCGLHIPSY 68

Query: 88  G-----TLLPASSAKYWNTMPLTSLSLLSNRRLYSSASSGKVDINGDAGVPAATSGVDVD 147
           G     T LPASS KYWNT+PLTS+  LSN R YSS S  K DI GD GVPAA++GV+ D
Sbjct: 69  GTHRIDTFLPASSMKYWNTIPLTSIGNLSNYRNYSSTSGRKADITGDTGVPAASAGVEPD 128

Query: 148 ISNPSDVGSNLLERAKDVWQGALDAASFTGQKAREVYDELSPYADKLLDSHPYLRNVIVP 207
           ISNP DVG +LLE+ KDVWQ A+DAASFTGQKA+EV DELSP+ DKLLDSHPYL+NVIVP
Sbjct: 129 ISNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVP 188

Query: 208 VSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAILSGSISVEEIPYEKSFWGALEDPLRYL 267
           VSMTL  TLLAW VMPRLLRRFHKYSMR PVAI+SG++  EEIPYEKSFWGALEDPLRYL
Sbjct: 189 VSMTLAGTLLAWAVMPRLLRRFHKYSMRSPVAIISGALPSEEIPYEKSFWGALEDPLRYL 248

Query: 268 VTFFAFSQIGTMVAPTAVASEFVSQACRGAVILSLVWFIYRWKTNVLSRALVSQNFARLE 327
           VTFFAFSQIG +VAPTAVA EFVSQACRGAVILSLVWFIYRWKTNV SRAL +Q  A L+
Sbjct: 249 VTFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVFSRALATQTSAGLD 308

Query: 328 RERLITLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGNFLSGL 387
           R+RL+TLDKVSSV LFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGN LSGL
Sbjct: 309 RDRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGNILSGL 368

Query: 388 TMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQ 447
           TMQFSQPFSLGDTIKAG IEGQVVEMGLMTTSLLSAEKFP+VVPNSLFSSQVIINKSRAQ
Sbjct: 369 TMQFSQPFSLGDTIKAGTIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQ 428

Query: 448 WRAITKKIPLQVDDLSTVSQISDYIKSMLRSHPKVFLGKEAPYAFLSQIESTYAELTIGC 507
           WRAI K++PLQVDD+STVSQI+D IK ML SHPKVFLGKEAPY FLSQIESTYAELT+GC
Sbjct: 429 WRAIVKRVPLQVDDISTVSQITDDIKKMLMSHPKVFLGKEAPYCFLSQIESTYAELTVGC 488

Query: 508 NLQKMGKEELFSTEQDILLQSVQIIKENGATLGTTMSDWTNK 544
           NL+KMGK+E FSTEQDILLQSVQIIK  GATLG+T+SDWTNK
Sbjct: 489 NLKKMGKDESFSTEQDILLQSVQIIKARGATLGSTVSDWTNK 521

BLAST of Sed0023991 vs. TAIR 10
Match: AT4G00290.1 (Mechanosensitive ion channel protein )

HSP 1 Score: 533.5 bits (1373), Expect = 2.0e-151
Identity = 270/428 (63.08%), Postives = 347/428 (81.07%), Query Frame = 0

Query: 115 YSSASSGKVDINGDAGVPAATSGVDVDISNPSDVGSNLLERAKDVWQGALDAASFTGQKA 174
           Y+ A S K D  G       T   D      +  G++ +E+AKDV Q ++DA + T +K 
Sbjct: 75  YARAFSSKSDDFGSIVASGVTGSGD-----GNGNGNDWVEKAKDVLQTSVDAVTETAKKT 134

Query: 175 REVYDELSPYADKLLDSHPYLRNVIVPVSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAI 234
           ++V DE+ P+  + LDS+PYL++VIVPVS+T+T TL AWVVMPR+LRRFH Y+M+    +
Sbjct: 135 KDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTMTGTLFAWVVMPRILRRFHTYAMQSSAKL 194

Query: 235 LSGSISVEEIPYEKSFWGALEDPLRYLVTFFAFSQIGTMVAPTAVASEFVSQACRGAVIL 294
           L    S E++PYEKSFWGALEDP RYLVTF AF+QI  MVAPT +A+++ S   +GAVIL
Sbjct: 195 LPVGFSNEDVPYEKSFWGALEDPARYLVTFIAFAQIAAMVAPTTIAAQYFSPTVKGAVIL 254

Query: 295 SLVWFIYRWKTNVLSRALVSQNFARLERERLITLDKVSSVGLFAIGLMALAEASGVAVQS 354
           SLVWF+YRWKTNV++R L +++F  L+RE+++TLDKVSSVGLFAIGLMA AEA GVAVQS
Sbjct: 255 SLVWFLYRWKTNVITRMLSAKSFGGLDREKVLTLDKVSSVGLFAIGLMASAEACGVAVQS 314

Query: 355 MLTVGGIGGVATAFAARDVLGNFLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSL 414
           +LTVGG+GGVATAFAARD+LGN LSGL+MQFS+PFS+GDTIKAG++EGQV+EMGL TTSL
Sbjct: 315 ILTVGGVGGVATAFAARDILGNVLSGLSMQFSRPFSMGDTIKAGSVEGQVIEMGLTTTSL 374

Query: 415 LSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPLQVDDLSTVSQISDYIKSMLRSHP 474
           L+AEKFP++VPNSLFSSQVI+NKSRAQWRAI  KIPLQ+DDL  + QIS+ IK MLRS+ 
Sbjct: 375 LNAEKFPVLVPNSLFSSQVIVNKSRAQWRAIASKIPLQIDDLDMIPQISNEIKEMLRSNT 434

Query: 475 KVFLGKEAPYAFLSQIESTYAELTIGCNLQKMGKEELFSTEQDILLQSVQIIKENGATLG 534
           KVFLGKEAP+ +LS++E ++AELTIGCNL +MGKEEL++T+Q++LL++V+IIK++G +LG
Sbjct: 435 KVFLGKEAPHCYLSRVEKSFAELTIGCNLIRMGKEELYNTQQEVLLEAVKIIKKHGVSLG 494

Query: 535 TTMSDWTN 543
           TT   W N
Sbjct: 495 TT---WDN 494

BLAST of Sed0023991 vs. TAIR 10
Match: AT4G00234.1 (BEST Arabidopsis thaliana protein match is: Mechanosensitive ion channel protein (TAIR:AT4G00290.1); Has 57 Blast hits to 57 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 56; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 262.7 bits (670), Expect = 6.6e-70
Identity = 136/245 (55.51%), Postives = 182/245 (74.29%), Query Frame = 0

Query: 115 YSSASSGKVDINGDAGVPAATSGVDVDISNPSDVGSNLLERAKDVWQGALDAASFTGQKA 174
           Y+ A S K D  G       T   D +       G++ +E+AKDV Q +++A + T +K 
Sbjct: 25  YARAFSSKSDDFGSIVASGVTGSGDGN-------GNDWVEKAKDVVQTSVNAVAETAKKT 84

Query: 175 REVYDELSPYADKLLDSHPYLRNVIVPVSMTLTATLLAWVVMPRLLRRFHKYSMRGPVAI 234
           ++V DE+ P+  + LDS+PYL++VIVPVS+T+T TL AW+VMPR+LRRFH Y+M+    +
Sbjct: 85  KDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTVTGTLFAWIVMPRILRRFHTYAMQSSAKL 144

Query: 235 LSGSISVEEIPYEKSFWGALEDPLRYLVTFFAFSQIGTMVAPTAVASEFVSQACRGAVIL 294
           L    S E+IPYEKSFWGALEDP RYLVTF AF+QI  MVAPT +A+++ S   +GAVIL
Sbjct: 145 LPVGFSNEDIPYEKSFWGALEDPARYLVTFIAFAQIAAMVAPTTIAAQYFSPTVKGAVIL 204

Query: 295 SLVWFIYRWKTNVLSRALVSQNFARLERERLITLDKVSSVGLFAIGLMALAEASGVAVQS 354
           S+VWF+YRWKTNV++R L +++F  L+R++++TLDKVSSVGLFAIGLMA AEA GVAVQS
Sbjct: 205 SVVWFLYRWKTNVITRMLSAKSFGGLDRDKVLTLDKVSSVGLFAIGLMASAEACGVAVQS 262

Query: 355 MLTVG 360
           +LTVG
Sbjct: 265 ILTVG 262

BLAST of Sed0023991 vs. TAIR 10
Match: AT1G58200.1 (MSCS-like 3 )

HSP 1 Score: 72.4 bits (176), Expect = 1.3e-12
Identity = 55/198 (27.78%), Postives = 99/198 (50.00%), Query Frame = 0

Query: 346 EASGVAVQSMLTVGGIGGVATAFAARDVLGNFLSGLTMQFSQPFSLGDTI--KAGAIE-- 405
           E  G + Q  LT GG+G V    A R++L NFLS + +  ++PF L + I  K G  E  
Sbjct: 257 ELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKIGGYEVS 316

Query: 406 GQVVEMGLMTTSLLSA-EKFPIVVPNSLFSSQVIIN-KSRAQWRAITKKIPLQVDDLSTV 465
           G V ++G  + +++   ++  + +PN  FS  ++ N   +  WR I   + +   D+S +
Sbjct: 317 GTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWR-IKTHLAISHLDVSKI 376

Query: 466 SQISDYIKSMLRSHPKVFLGKEAPYAFLSQI--ESTYAELTIGCNLQKMGKEELFSTEQD 525
           + I   ++ +L  +P++   K     FL  I  E+    + I C ++    EE    ++ 
Sbjct: 377 NNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILISCFVKTSRFEEYLCVKEA 436

Query: 526 ILLQSVQIIKENGATLGT 536
           +LL  + +I+ +GA L T
Sbjct: 437 VLLDLLTVIRHHGARLAT 453

BLAST of Sed0023991 vs. TAIR 10
Match: AT1G58200.2 (MSCS-like 3 )

HSP 1 Score: 72.4 bits (176), Expect = 1.3e-12
Identity = 55/198 (27.78%), Postives = 99/198 (50.00%), Query Frame = 0

Query: 346 EASGVAVQSMLTVGGIGGVATAFAARDVLGNFLSGLTMQFSQPFSLGDTI--KAGAIE-- 405
           E  G + Q  LT GG+G V    A R++L NFLS + +  ++PF L + I  K G  E  
Sbjct: 257 ELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKIGGYEVS 316

Query: 406 GQVVEMGLMTTSLLSA-EKFPIVVPNSLFSSQVIIN-KSRAQWRAITKKIPLQVDDLSTV 465
           G V ++G  + +++   ++  + +PN  FS  ++ N   +  WR I   + +   D+S +
Sbjct: 317 GTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWR-IKTHLAISHLDVSKI 376

Query: 466 SQISDYIKSMLRSHPKVFLGKEAPYAFLSQI--ESTYAELTIGCNLQKMGKEELFSTEQD 525
           + I   ++ +L  +P++   K     FL  I  E+    + I C ++    EE    ++ 
Sbjct: 377 NNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILISCFVKTSRFEEYLCVKEA 436

Query: 526 ILLQSVQIIKENGATLGT 536
           +LL  + +I+ +GA L T
Sbjct: 437 VLLDLLTVIRHHGARLAT 453

BLAST of Sed0023991 vs. TAIR 10
Match: AT5G10490.1 (MSCS-like 2 )

HSP 1 Score: 66.6 bits (161), Expect = 6.9e-11
Identity = 56/212 (26.42%), Postives = 102/212 (48.11%), Query Frame = 0

Query: 332 SSVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGNFLSGLTMQFSQPFSL 391
           S+V + A+ L    E  G + Q  LT GG+G V    A R++L NFLS + +  ++PF L
Sbjct: 243 SAVWVAAVSL--FMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 302

Query: 392 GD----TIKAGAIEGQVVEMGLMTTSLLSAE-KFPIVVPNSLFSSQVIIN-KSRAQWRAI 451
            +     I+   + G V  +G  + +++  E +  I +PN  F+  V+ N   +  WR I
Sbjct: 303 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWR-I 362

Query: 452 TKKIPLQVDDLSTVSQISDYIKSMLRSHPKVFLGKEAPYAFLSQI--ESTYAELTIGCNL 511
              + +   D++ ++ I   ++ +L  +P V   +     FL  +  E+    + I C +
Sbjct: 363 KTHLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFV 422

Query: 512 QKMGKEELFSTEQDILLQSVQIIKENGATLGT 536
           +    EE    ++ ILL  +++I  + A L T
Sbjct: 423 KTSHHEEYLGVKEAILLDLLRVISHHRARLAT 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022969688.13.8e-23782.49mechanosensitive ion channel protein 1, mitochondrial-like [Cucurbita maxima][more]
XP_023538305.13.8e-23782.49mechanosensitive ion channel protein 1, mitochondrial-like [Cucurbita pepo subsp... [more]
KAG6586069.11.2e-23582.11Mechanosensitive ion channel protein 1, mitochondrial, partial [Cucurbita argyro... [more]
XP_022937845.12.1e-23582.49mechanosensitive ion channel protein 1, mitochondrial-like [Cucurbita moschata][more]
XP_022147379.12.1e-23580.95mechanosensitive ion channel protein 1, mitochondrial-like [Momordica charantia]... [more]
Match NameE-valueIdentityDescription
Q8VZL42.8e-15063.08Mechanosensitive ion channel protein 1, mitochondrial OS=Arabidopsis thaliana OX... [more]
P0AEB64.1e-2427.00Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=833... [more]
P0AEB54.1e-2427.00Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) O... [more]
O251703.8e-1430.54Uncharacterized MscS family protein HP_0415 OS=Helicobacter pylori (strain ATCC ... [more]
Q9ZKG75.0e-1430.54Uncharacterized MscS family protein jhp_0969 OS=Helicobacter pylori (strain J99 ... [more]
Match NameE-valueIdentityDescription
A0A6J1I1Q41.8e-23782.49mechanosensitive ion channel protein 1, mitochondrial-like OS=Cucurbita maxima O... [more]
A0A6J1D2801.0e-23580.95mechanosensitive ion channel protein 1, mitochondrial-like OS=Momordica charanti... [more]
A0A6J1FHY51.0e-23582.49mechanosensitive ion channel protein 1, mitochondrial-like OS=Cucurbita moschata... [more]
A0A0A0L6857.3e-22680.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G121740 PE=3 SV=1[more]
A0A1S3AVZ33.1e-22479.50mechanosensitive ion channel protein 1, mitochondrial OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT4G00290.12.0e-15163.08Mechanosensitive ion channel protein [more]
AT4G00234.16.6e-7055.51BEST Arabidopsis thaliana protein match is: Mechanosensitive ion channel protein... [more]
AT1G58200.11.3e-1227.78MSCS-like 3 [more]
AT1G58200.21.3e-1227.78MSCS-like 3 [more]
AT5G10490.16.9e-1126.42MSCS-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 330..398
e-value: 1.1E-12
score: 48.0
NoneNo IPR availableGENE3D1.10.287.1260coord: 284..386
e-value: 3.0E-14
score: 55.1
NoneNo IPR availablePANTHERPTHR30566:SF5MECHANOSENSITIVE ION CHANNEL PROTEIN 1, MITOCHONDRIAL-RELATEDcoord: 93..399
NoneNo IPR availablePANTHERPTHR30566YNAI-RELATED MECHANOSENSITIVE ION CHANNELcoord: 93..399
IPR011014Mechanosensitive ion channel MscS, transmembrane-2SUPERFAMILY82861Mechanosensitive channel protein MscS (YggB), transmembrane regioncoord: 289..371

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0023991.1Sed0023991.1mRNA
Sed0023991.2Sed0023991.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006811 ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane