Sed0023845 (gene) Chayote v1

BLAST of Sed0023845 vs. NCBI nr
Match: XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.1 putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 954.1 bits (2465), Expect = 5.2e-274
Identity = 460/551 (83.48%), Postives = 498/551 (90.38%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEARYTWSETHRRCLQLASSL-SSA 60
           MEDLKP PANSSPLTP+GFLERAA +YGDCPSIIY    YTW+ETHRRCLQLASSL S  
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDEA 120
           I RG VVSV+SPNT AMYELHFAVPMAGAVLNTINLRLDARTISV+L HC++ L+FVD+A
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDPEF 180
           S  LI  A +L PP+ KRPPL+LI D  AA +  ++S V    DFVDSYE+MV+KGDP F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 KWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLPMF 240
           +W+RPAS+WDPIVLNYTSGTTSSPKGVVHCHRGIF V+LDSL+EWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDNRPL 300
           HANGWS+PWGIAA+GGTN+CIRKFDA AIF+LI R+HVTH+CGAPVVLNMLTNSPDNRPL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEERAR 360
           DRPVKILTAGAPPPAAVL RTE+LGFDV HGYGLTET GLVICCSWK  W+RLPA ERAR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSMTE 420
           LKARQGVRT AMAVADV+DP TGKSVK DG SIGEI LRG S+MLGYLKDP ATAK+MT 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVARPD 480
           EGWF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAV+EAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           EFWGETPCAFVSL+EGL RRPTE EI+EYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLRQMAKSMGP 551
           VLR+MAKSMGP
Sbjct: 541 VLREMAKSMGP 551

BLAST of Sed0023845 vs. NCBI nr
Match: XP_023550909.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 945.3 bits (2442), Expect = 2.4e-271
Identity = 458/555 (82.52%), Postives = 498/555 (89.73%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEARYTWSETHRRCLQLASSL-SSA 60
           MEDLKP PANSSPLTP+GFLERAA +YGDC SIIY    YTW+ETHRRCLQLASSL S  
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCRSIIYDSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDEA 120
           I RG VVSV+SPNT AMYELHFAVPMAGAVLNTINLRLDARTISV+L HC++ L+FVD+A
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITL----DFVDSYEDMVRKG 180
           S  LI  A +L PP+ KRPPL+LI D  AA +  +SS    ++    DFVDSYE+MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVLNRADFVDSYEEMVQKG 180

Query: 181 DPEFKWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWT 240
           DP F+W+RPAS+WDPIVLNYTSGTTSSPKGVVHCHRGIF V+LDSL+EWGVPK+SVYLWT
Sbjct: 181 DPLFQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWT 240

Query: 241 LPMFHANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPD 300
           LPMFHANGWS+PWGIAA+GGTN+CIRKFDA AIF+LI R+HVTH+CGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NRPLDRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAE 360
           NRPLDRPVKILTAGAPPPAAVL RTE+LGFDV HGYGLTET GLVICCSWK  W+RLPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 ERARLKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAK 420
           +RARLKARQGVRT AMAVADV+DP TGKSVK DG SIGEI LRG S+MLGYLKDP ATAK
Sbjct: 361 DRARLKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 SMTEEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVV 480
           +MT EGWF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAV+EAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540
           ARPDEFWGETPCAFVSL+EGL RRPTE EI+EYCRGKLPRFMVPKTVVFTAELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540

Query: 541 IQKFVLRQMAKSMGP 551
           IQKFVLR+MAKSMGP
Sbjct: 541 IQKFVLREMAKSMGP 555

BLAST of Sed0023845 vs. NCBI nr
Match: XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 941.8 bits (2433), Expect = 2.7e-270
Identity = 460/561 (82.00%), Postives = 497/561 (88.59%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEARYTWSETHRRCLQLASSL-SSA 60
           MEDLKP PANSSPLTP+GFLERAA +YGDCPSIIY    YTW+ETHRRCLQLASSL S  
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDEA 120
           I RG VVSV+SPNTPAMYELHFAVPMAGAVLNTINLRLD+RTISV+L HC++ L+FVD+A
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTS----SPVPITLDFVDSYEDMVRKG 180
           S  LI  A +L PP+ KRPPL+LI D  AA +  +S    S V    DFVDSYE+MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 DPEFKWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWT 240
           DP F+W+RP S+WDPIVLNYTSGTTSSPKGVVHCHRGIF V+LDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPMFHANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPD 300
           LPMFHANGWS+PWGIAA+GGTN+CIRKFDA  IF+LI R+HVTH+CGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NRPLDRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAE 360
           NRPLDRPVKILTAGAPPPAAVL RTE+LGFDV HGYGLTET GLVICCSWK  W+RLPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 ERARLKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAK 420
           ERARLKARQGVRT AMAVADV+ P TGKSVK DG SIGEI LRG S+MLGYLKDP ATAK
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 SMTEEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVV 480
           +MT EGWF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAV+EAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540
           ARPDEFWGETPCAFVSL+EGL RRPTE EI+EYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 IQKFVLRQMAKSMGPP-RGRL 556
           IQKFVLR+MAKSMGP  R RL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of Sed0023845 vs. NCBI nr
Match: XP_023554638.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 912.5 bits (2357), Expect = 1.7e-261
Identity = 446/557 (80.07%), Postives = 491/557 (88.15%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEARYTWSETHRRCLQLASSLSSA- 60
           MEDLKPR  NSSPLTP+GFLERAA IYGD PSIIYG+  YTWSETH RCLQLASSLSS+ 
Sbjct: 39  MEDLKPRTPNSSPLTPIGFLERAATIYGDSPSIIYGDTTYTWSETHHRCLQLASSLSSSG 98

Query: 61  IHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDEA 120
           I RG VVSV+SPNTP MYELHFAVPMAGA+LNTIN RLDARTIS LL H +SKLVFVD+A
Sbjct: 99  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQA 158

Query: 121 SSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFT-SSPVPITLDFVDSYEDMVRKGDPE 180
             +LIF A +L PP+   P LVLI D  AAAEE + SS V I +DFVDSYE+M++KGD +
Sbjct: 159 YCALIFDALALFPPETNPPSLVLIDDDAAAAEEESLSSSVSIRIDFVDSYEEMIQKGDSK 218

Query: 181 FKWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLPM 240
           F+W+RP S+WDPIVLNYTSGTTSSPKGVVHCHRG+F+ + DSLLEWGVPK SVYLWTLPM
Sbjct: 219 FQWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPM 278

Query: 241 FHANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDNRP 300
           FH NGWS+ WGIAA+GGTN+CIRKFDA  IFSLIRRHHVTHMC APVVLNMLTNS D+RP
Sbjct: 279 FHINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDDRP 338

Query: 301 LDRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEERA 360
           LDRPVKILT GAP PAAVL R E+LGFDV HGYGLTETAG+VICCSWK +W+RLPA E+A
Sbjct: 339 LDRPVKILTGGAPLPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQA 398

Query: 361 RLKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSMT 420
           RLKARQGVRTAA+A  DVVD  TGKSVK DGVSIGEI +RG SIM+GY KD  ATAKSMT
Sbjct: 399 RLKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMT 458

Query: 421 EEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVARP 480
           +EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNP+V+EAAVVARP
Sbjct: 459 DEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARP 518

Query: 481 DEFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQK 540
           DEFWGETPCAFVSLREGL R+PTEEEI+EYCRGKLPRFMVPKTVVF AELPKTSTGKI+K
Sbjct: 519 DEFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGKIKK 578

Query: 541 FVLRQMAKSMGPPRGRL 556
           +VL+++A S+GP   RL
Sbjct: 579 YVLKEIAMSIGPKPSRL 595

BLAST of Sed0023845 vs. NCBI nr
Match: XP_022990524.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 905.2 bits (2338), Expect = 2.8e-259
Identity = 442/556 (79.50%), Postives = 487/556 (87.59%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEARYTWSETHRRCLQLASSLSSA- 60
           MEDLKPRP NS+PLTP+GFLERAA IYG+ PSIIYG+  YTWSETH RCLQLASSLSS+ 
Sbjct: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSG 98

Query: 61  IHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDEA 120
           I RG VVSV+SPNTP MYELHFAVPMAGA+LNTIN RLDARTIS LL H +SKLVFVD+A
Sbjct: 99  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQA 158

Query: 121 SSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDPEF 180
             +LI  A +L PP+   P LVLI D DAAAEE  SS V I +DF DSYE+M++KGD +F
Sbjct: 159 YCALISDALALFPPETNPPSLVLI-DDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKF 218

Query: 181 KWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLPMF 240
           +W+RP S+WDPIVLNYTSGTTSSPKGVVHCHRG+F+ + DSLLEWGVPK SVYLWTL MF
Sbjct: 219 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMF 278

Query: 241 HANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDNRPL 300
           H NGWS+ WGIAA+GGTNICIRKFDA  IFSLIRRHHVTHMC APVVLNMLTNS DNR L
Sbjct: 279 HINGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSL 338

Query: 301 DRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEERAR 360
           DRPVKILT G+PPPAAVL R E+LGFDV HGYGLTETAG+VICCSWK +W+RLPA  +AR
Sbjct: 339 DRPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQAR 398

Query: 361 LKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSMTE 420
           LKARQGVRTAA+A  DVVD  TGKSVK DGVSIGEI +RG SIM+GY KD  ATAKS+T+
Sbjct: 399 LKARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITD 458

Query: 421 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVARPD 480
           EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LYTNP V+EAAVVARPD
Sbjct: 459 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPD 518

Query: 481 EFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           EFWGETPCAFVSLREGL R+PTEEEI+EYCRGKLPRFMVPKTVVF AELPKTSTGKI+K+
Sbjct: 519 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKY 578

Query: 541 VLRQMAKSMGPPRGRL 556
           VL++MA S+GP   RL
Sbjct: 579 VLKEMAMSIGPKPSRL 593

BLAST of Sed0023845 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 1.8e-237
Identity = 386/550 (70.18%), Postives = 458/550 (83.27%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEARYTWSETHRRCLQLASSLSS-A 60
           MEDLKPRPA+SSPLTPLGFLERAA +YGDC S++Y    YTWS+THRRCL LASS++S  
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 61  IHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDEA 120
           I  G VVSVL+PN P MYELHFAVPMAGA+LN +NLRLDARTIS+LL H +SKL+FVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 121 SSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDPEF 180
           S  LI  A +L P  A  P LV + D     E  + +   +  +F  SY+D++ +GDP+F
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMAD-----ESESGNSSELGKEFFCSYKDLIDRGDPDF 180

Query: 181 KWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLPMF 240
           KWV P S+WDP++LNYTSGTTSSPKGVVHCHRGIF++++DSL++WGVPKQ VYLWTLPMF
Sbjct: 181 KWVMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDNRPL 300
           HANGWSYPWG+AA+GGTNIC+RKFD+  I+ +I+RH VTHMCGAPVVLNML+N+P + PL
Sbjct: 241 HANGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEERAR 360
              V+I+TAGAPPP+AVL RTE+LGF V HGYGLTETAGLV+ C+WK +W+ LPA ERAR
Sbjct: 301 KTTVQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERAR 360

Query: 361 LKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSMTE 420
           LK+RQGV T      DVVDP TG +VK DG ++GE+ LRG S+MLGYLKDP  TAKSMT 
Sbjct: 361 LKSRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTA 420

Query: 421 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVARPD 480
           +GWF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY++P + EAAVVARPD
Sbjct: 421 DGWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           EFWGETPCAFVSL++GL ++PTE+EI+EYCR KLPR+MVPKTVVF  ELPKTSTGK+QKF
Sbjct: 481 EFWGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKF 540

Query: 541 VLRQMAKSMG 550
           +LR MA+ MG
Sbjct: 541 ILRDMARGMG 545

BLAST of Sed0023845 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 6.7e-224
Identity = 377/554 (68.05%), Postives = 450/554 (81.23%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYG-EARYTWSETHRRCLQLASSLSS- 60
           ME +KP  ANS PLTP+GFLERAA +YGDC SI+YG    YTW ET+ RCL++ASSLSS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  AIHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDE 120
            I R DVVSVLSPNTPAMYEL FAVPM+GA+LN IN RLDART+SVLL HC SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDPE 180
            S  L   A S++  D   P LV I D +   EE   + V     F  +Y+D++ +GD +
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIADKE---EEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FKWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLPM 240
           FKW+RP S+WDP+VLNYTSGTTS+PKGVVHCHRGIF++S+DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDNRP 300
           FHANGWSYPWGIAA+GGTN+C+RKFDA  I+ LIR H VTHMCGAPVVLNML+ + + +P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEERA 360
           L+RPV ILTAGAPPPAAVLLR E++GF + HGYGLTETAGL + C+WKPQW+RLPA +RA
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSMT 420
           RLKARQGVRT      DVVDP +G+SV+ +G ++GEI +RG SIMLGYLKDP  T K++ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420

Query: 421 EEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVARP 480
           + GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAV+E AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQK 540
           D FWGETPCAFVSL+ GL +RPTE E++EYCR K+P++MVPKTV F  ELPKTSTGK+ K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 FVLRQMAKSMGPPR 553
           FVLR++AK MG  R
Sbjct: 541 FVLREIAKKMGTTR 548

BLAST of Sed0023845 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 1.4e-221
Identity = 375/552 (67.93%), Postives = 447/552 (80.98%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEARYTWSETHRRCLQLASSLSS-- 60
           ME+LKPR ANS PLTPLGFLERAA +YGDC S++Y +  YTWS+TH RCL++AS + S  
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 61  AIHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDE 120
            + +G VVSV++PN PAMYEL+FAVPMAG VLN IN RL+A TISV+L H +SKLVFVD+
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 121 ASSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDPE 180
            S  L+  A SL P +   P LVLI D +   E  T   V     FV  YED+V KGD +
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIAD-NITGENLT---VEDREHFVCCYEDLVEKGDDK 180

Query: 181 -FKWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLP 240
            FKWVRP S+WDPIVLNYTSGTTSSPKGVVH HR +F+V+LDSL++WGVPKQ VYLWTLP
Sbjct: 181 TFKWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLP 240

Query: 241 MFHANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDNR 300
           MFHANGW Y WGIAA+GGTNIC+R+FD   IF+LIRRH VTHMC AP+VLNML+NSP+  
Sbjct: 241 MFHANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAE 300

Query: 301 PLDRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEER 360
           PL  PV ++T GAPPPAAVLLRTE+LGF + HGYG+TE  G+V+ C+WK +W+RLPA E+
Sbjct: 301 PLPNPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQ 360

Query: 361 ARLKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSM 420
           ARLK+RQGVRTAAM   DVVDP +G SVK DG+++GEI L+G SIMLGYLK+ AATAK +
Sbjct: 361 ARLKSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCI 420

Query: 421 TEEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVAR 480
             +GWF TGD+ VMHPDGYLEIKDRSKDVIISGGEN+SSVEVES LY++PAV EAAVVA 
Sbjct: 421 RADGWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVAC 480

Query: 481 PDEFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQ 540
           PDE+WGETP AFV+L++G+R RPTE+EI+EYCR KL  FMVPK VVF  +LPKTSTGKIQ
Sbjct: 481 PDEYWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQ 540

Query: 541 KFVLRQMAKSMG 550
           KF L+++ K+MG
Sbjct: 541 KFTLKEIVKTMG 548

BLAST of Sed0023845 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 5.9e-220
Identity = 366/551 (66.42%), Postives = 447/551 (81.13%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYG-EARYTWSETHRRCLQLASSLSS- 60
           ME++KP  ANS PLTP+GFLERAA +YGDC SI+YG    YTW ET+ RCL++ASSLSS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  AIHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDE 120
            I R DVVSVLSPNTPAMYEL FAVPM+GA+LN IN RLDART+SVLL HC+SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDPE 180
            S  L   A S++  D   P LV+I D +   EE   + V     F  +Y+D++ +GDP 
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIADKE---EEGGVADVADLSKFSYTYDDLIERGDPG 180

Query: 181 FKWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLPM 240
           FKW+RP S+WDP+VLNYTSGTTS+PKGVVHCHRGIF++S+DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDNRP 300
           FH+NGW+ PWGIAA+GGTN+C+RKFDA  I+ LIR H VTHMCGAPVVLNML+ + +++P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEERA 360
           L+ PV ILTAG+PPPA VLLR E++GF + HGYGLTETAG+++ C+WKP+W+ LPA +RA
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSMT 420
           RLKARQGVRT      DVVDP +G SV+ +G ++GEI +RG S+MLGYLKDP  T K++ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420

Query: 421 EEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVARP 480
           + GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAV+E AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQK 540
           DEFWGETPCAFVSL+ G   +PTEEE+MEYCR K+P++MVPKTV F  ELPK+STGK+ K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 FVLRQMAKSMG 550
           FVLR +AK MG
Sbjct: 541 FVLRDIAKKMG 545

BLAST of Sed0023845 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 1.9e-210
Identity = 360/551 (65.34%), Postives = 439/551 (79.67%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEAR-YTWSETHRRCLQLASSLSS- 60
           MEDLKP  ANS PLT LGFLERAA +YGDC SI+YG +  YTW ET+ RCL +AS+LSS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  AIHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDE 120
            I R DVVSVLS NTP MYEL F+VPM+GA+LN IN RLDART+SVLL HC+SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDPE 180
             S L   A ++L      P LVLI + +   EE   + V     F   Y D++ +G+P+
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEE---EEEGGAEVTERSKFCYLYSDLITRGNPD 180

Query: 181 FKWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLPM 240
           FKW+RP S+WDPIV+NYTSGTTSSPKGVVHCHRGIF+++LDSL +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPD-NR 300
           FHANGW+YPWGIAA+GGTN+C+RK  A +I+ LIR H VTHM GAP+VL +L+ S + ++
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEER 360
           PL  PV  LTAG+ PPA VLLR E+LGF V HGYGLTETAG+++ C+WKP W+RLPA ++
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSM 420
           A+LK+RQGVRT   +  DVVDP +G+SV+ DG ++GEI LRG SIMLGYLK+P  T  S 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 TEEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVAR 480
            + GWF TGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNPAV+EAAVVAR
Sbjct: 421 -KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQ 540
           PDEFWGETPCAFVSL+ GL R+PT++EI+EYC+ K+PR+M PKTV F  ELPKTSTGKI 
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KFVLRQMAKSM 549
           K +L+++AK+M
Sbjct: 541 KSLLKEIAKNM 544

BLAST of Sed0023845 vs. ExPASy TrEMBL
Match: A0A6J1FL76 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445243 PE=4 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 2.5e-274
Identity = 460/551 (83.48%), Postives = 498/551 (90.38%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEARYTWSETHRRCLQLASSL-SSA 60
           MEDLKP PANSSPLTP+GFLERAA +YGDCPSIIY    YTW+ETHRRCLQLASSL S  
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDEA 120
           I RG VVSV+SPNT AMYELHFAVPMAGAVLNTINLRLDARTISV+L HC++ L+FVD+A
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDPEF 180
           S  LI  A +L PP+ KRPPL+LI D  AA +  ++S V    DFVDSYE+MV+KGDP F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 KWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLPMF 240
           +W+RPAS+WDPIVLNYTSGTTSSPKGVVHCHRGIF V+LDSL+EWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDNRPL 300
           HANGWS+PWGIAA+GGTN+CIRKFDA AIF+LI R+HVTH+CGAPVVLNMLTNSPDNRPL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEERAR 360
           DRPVKILTAGAPPPAAVL RTE+LGFDV HGYGLTET GLVICCSWK  W+RLPA ERAR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSMTE 420
           LKARQGVRT AMAVADV+DP TGKSVK DG SIGEI LRG S+MLGYLKDP ATAK+MT 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVARPD 480
           EGWF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAV+EAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           EFWGETPCAFVSL+EGL RRPTE EI+EYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLRQMAKSMGP 551
           VLR+MAKSMGP
Sbjct: 541 VLREMAKSMGP 551

BLAST of Sed0023845 vs. ExPASy TrEMBL
Match: A0A6J1JTQ2 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489657 PE=4 SV=1)

HSP 1 Score: 941.8 bits (2433), Expect = 1.3e-270
Identity = 460/561 (82.00%), Postives = 497/561 (88.59%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEARYTWSETHRRCLQLASSL-SSA 60
           MEDLKP PANSSPLTP+GFLERAA +YGDCPSIIY    YTW+ETHRRCLQLASSL S  
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDEA 120
           I RG VVSV+SPNTPAMYELHFAVPMAGAVLNTINLRLD+RTISV+L HC++ L+FVD+A
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTS----SPVPITLDFVDSYEDMVRKG 180
           S  LI  A +L PP+ KRPPL+LI D  AA +  +S    S V    DFVDSYE+MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 DPEFKWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWT 240
           DP F+W+RP S+WDPIVLNYTSGTTSSPKGVVHCHRGIF V+LDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPMFHANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPD 300
           LPMFHANGWS+PWGIAA+GGTN+CIRKFDA  IF+LI R+HVTH+CGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NRPLDRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAE 360
           NRPLDRPVKILTAGAPPPAAVL RTE+LGFDV HGYGLTET GLVICCSWK  W+RLPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 ERARLKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAK 420
           ERARLKARQGVRT AMAVADV+ P TGKSVK DG SIGEI LRG S+MLGYLKDP ATAK
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 SMTEEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVV 480
           +MT EGWF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAV+EAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540
           ARPDEFWGETPCAFVSL+EGL RRPTE EI+EYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 IQKFVLRQMAKSMGPP-RGRL 556
           IQKFVLR+MAKSMGP  R RL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of Sed0023845 vs. ExPASy TrEMBL
Match: A0A6J1JS99 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487376 PE=4 SV=1)

HSP 1 Score: 905.2 bits (2338), Expect = 1.3e-259
Identity = 442/556 (79.50%), Postives = 487/556 (87.59%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEARYTWSETHRRCLQLASSLSSA- 60
           MEDLKPRP NS+PLTP+GFLERAA IYG+ PSIIYG+  YTWSETH RCLQLASSLSS+ 
Sbjct: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSG 98

Query: 61  IHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDEA 120
           I RG VVSV+SPNTP MYELHFAVPMAGA+LNTIN RLDARTIS LL H +SKLVFVD+A
Sbjct: 99  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQA 158

Query: 121 SSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDPEF 180
             +LI  A +L PP+   P LVLI D DAAAEE  SS V I +DF DSYE+M++KGD +F
Sbjct: 159 YCALISDALALFPPETNPPSLVLI-DDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKF 218

Query: 181 KWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLPMF 240
           +W+RP S+WDPIVLNYTSGTTSSPKGVVHCHRG+F+ + DSLLEWGVPK SVYLWTL MF
Sbjct: 219 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMF 278

Query: 241 HANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDNRPL 300
           H NGWS+ WGIAA+GGTNICIRKFDA  IFSLIRRHHVTHMC APVVLNMLTNS DNR L
Sbjct: 279 HINGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSL 338

Query: 301 DRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEERAR 360
           DRPVKILT G+PPPAAVL R E+LGFDV HGYGLTETAG+VICCSWK +W+RLPA  +AR
Sbjct: 339 DRPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQAR 398

Query: 361 LKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSMTE 420
           LKARQGVRTAA+A  DVVD  TGKSVK DGVSIGEI +RG SIM+GY KD  ATAKS+T+
Sbjct: 399 LKARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITD 458

Query: 421 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVARPD 480
           EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LYTNP V+EAAVVARPD
Sbjct: 459 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPD 518

Query: 481 EFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           EFWGETPCAFVSLREGL R+PTEEEI+EYCRGKLPRFMVPKTVVF AELPKTSTGKI+K+
Sbjct: 519 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKY 578

Query: 541 VLRQMAKSMGPPRGRL 556
           VL++MA S+GP   RL
Sbjct: 579 VLKEMAMSIGPKPSRL 593

BLAST of Sed0023845 vs. ExPASy TrEMBL
Match: A0A6J1HFJ6 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111463113 PE=4 SV=1)

HSP 1 Score: 903.7 bits (2334), Expect = 3.9e-259
Identity = 441/556 (79.32%), Postives = 485/556 (87.23%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEARYTWSETHRRCLQLASSLSSA- 60
           MEDLKPR  NS+PLTP+GFLERAA IYGD PSIIYG   YTWSETH RCLQLASSLSS+ 
Sbjct: 39  MEDLKPRTPNSAPLTPIGFLERAATIYGDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 98

Query: 61  IHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDEA 120
           I RG VVSV+SPNTP MYELHFAVPMAGA+LNTIN RLDARTIS LLSH +SKLVFV +A
Sbjct: 99  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVHQA 158

Query: 121 SSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDPEF 180
             +LI  A +L P +   P LVLI D  AAAEE  SS V I +DFVDSYE+M++KGD +F
Sbjct: 159 YCALISDALALFPLETNPPSLVLIDDDAAAAEESLSSSVSIRIDFVDSYEEMIQKGDSKF 218

Query: 181 KWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLPMF 240
           +W+RP S+WDPIVLNYTSGTTSSPKGVVHCHRG+F+ + DSLLEWGVPK SVYLWTLPMF
Sbjct: 219 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMF 278

Query: 241 HANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDNRPL 300
           H NGWS+ WGIAA+GGTN+CIRKFDA  IFSLIRRHHVTHMC APVVLNMLTNS DNR L
Sbjct: 279 HINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSL 338

Query: 301 DRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEERAR 360
           DRPVKILT GAPPPAAVL R E+LGFDV HGYGLTETAG+VICCSWK +W+RLPA E+AR
Sbjct: 339 DRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQAR 398

Query: 361 LKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSMTE 420
           LKARQGVRTAA+A  DVVD  TGKSVK DGVSIGEI + G SIM+GY KD  ATAKSMT+
Sbjct: 399 LKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVSGGSIMVGYWKDREATAKSMTD 458

Query: 421 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVARPD 480
           EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNP+V+EAAVVARPD
Sbjct: 459 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARPD 518

Query: 481 EFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           EFWGETPCAFVSLREGL R+PTEEEI++YCRGKLPRFMVPKTVVF AELPKTSTGKI+K+
Sbjct: 519 EFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKY 578

Query: 541 VLRQMAKSMGPPRGRL 556
           VL+++A S+G    RL
Sbjct: 579 VLKEIAMSIGSKSSRL 594

BLAST of Sed0023845 vs. ExPASy TrEMBL
Match: A0A5D3D623 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00690 PE=4 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 8.7e-259
Identity = 432/557 (77.56%), Postives = 486/557 (87.25%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEARYTWSETHRRCLQLASSLSS-A 60
           MEDLKP PANS PLTP+GF++RAA IYGD  SIIY    YTWSETHRRCLQLASS+SS  
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDEA 120
           I RG VVSV++PNTPAMYELHFAVPM GA+LNTINLRLD RTIS+LL H +SKL+FVD+A
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDPEF 180
           S +LIF A +L PP+ KRP LVLI D  A  +    S V I  +FVDSYEDMV++GD +F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 KWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLPMF 240
           +W+RP S+WDPIVLNYTSGTTSSPKGVVHCHRGIF V+LDSLLEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDNRPL 300
           H NGWS+PWGIAA+GGTN+CIRKFDA  IFSLIRRHHVTH+CGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEERAR 360
           DRPVKILTAGAPPPA++LLRTE+LGF V HGYGLTE+AG +ICCSWK +W++LPA E+AR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSMTE 420
           LKARQGVR  A+   DV+DP T KSVK DG SIGEI +RG S+ LGYLKDP  TAK+M+E
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVARPD 480
           +GWF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NP V+EAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           EFWGETPCAF+SL+EGL R+PTEEEI+EYC+GKLPRFMVPKTV+F  E+PKT+TGKIQKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLRQMAKSMGP-PRGRL 556
           VLRQ AKS+GP P+ RL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of Sed0023845 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 778.1 bits (2008), Expect = 4.8e-225
Identity = 377/554 (68.05%), Postives = 450/554 (81.23%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYG-EARYTWSETHRRCLQLASSLSS- 60
           ME +KP  ANS PLTP+GFLERAA +YGDC SI+YG    YTW ET+ RCL++ASSLSS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  AIHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDE 120
            I R DVVSVLSPNTPAMYEL FAVPM+GA+LN IN RLDART+SVLL HC SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDPE 180
            S  L   A S++  D   P LV I D +   EE   + V     F  +Y+D++ +GD +
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIADKE---EEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FKWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLPM 240
           FKW+RP S+WDP+VLNYTSGTTS+PKGVVHCHRGIF++S+DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDNRP 300
           FHANGWSYPWGIAA+GGTN+C+RKFDA  I+ LIR H VTHMCGAPVVLNML+ + + +P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEERA 360
           L+RPV ILTAGAPPPAAVLLR E++GF + HGYGLTETAGL + C+WKPQW+RLPA +RA
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSMT 420
           RLKARQGVRT      DVVDP +G+SV+ +G ++GEI +RG SIMLGYLKDP  T K++ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420

Query: 421 EEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVARP 480
           + GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAV+E AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQK 540
           D FWGETPCAFVSL+ GL +RPTE E++EYCR K+P++MVPKTV F  ELPKTSTGK+ K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 FVLRQMAKSMGPPR 553
           FVLR++AK MG  R
Sbjct: 541 FVLREIAKKMGTTR 548

BLAST of Sed0023845 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 765.0 bits (1974), Expect = 4.2e-221
Identity = 366/551 (66.42%), Postives = 447/551 (81.13%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYG-EARYTWSETHRRCLQLASSLSS- 60
           ME++KP  ANS PLTP+GFLERAA +YGDC SI+YG    YTW ET+ RCL++ASSLSS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  AIHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDE 120
            I R DVVSVLSPNTPAMYEL FAVPM+GA+LN IN RLDART+SVLL HC+SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDPE 180
            S  L   A S++  D   P LV+I D +   EE   + V     F  +Y+D++ +GDP 
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIADKE---EEGGVADVADLSKFSYTYDDLIERGDPG 180

Query: 181 FKWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLPM 240
           FKW+RP S+WDP+VLNYTSGTTS+PKGVVHCHRGIF++S+DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDNRP 300
           FH+NGW+ PWGIAA+GGTN+C+RKFDA  I+ LIR H VTHMCGAPVVLNML+ + +++P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEERA 360
           L+ PV ILTAG+PPPA VLLR E++GF + HGYGLTETAG+++ C+WKP+W+ LPA +RA
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSMT 420
           RLKARQGVRT      DVVDP +G SV+ +G ++GEI +RG S+MLGYLKDP  T K++ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420

Query: 421 EEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVARP 480
           + GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAV+E AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQK 540
           DEFWGETPCAFVSL+ G   +PTEEE+MEYCR K+P++MVPKTV F  ELPK+STGK+ K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 FVLRQMAKSMG 550
           FVLR +AK MG
Sbjct: 541 FVLRDIAKKMG 545

BLAST of Sed0023845 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 733.4 bits (1892), Expect = 1.3e-211
Identity = 360/551 (65.34%), Postives = 439/551 (79.67%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEAR-YTWSETHRRCLQLASSLSS- 60
           MEDLKP  ANS PLT LGFLERAA +YGDC SI+YG +  YTW ET+ RCL +AS+LSS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  AIHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVDE 120
            I R DVVSVLS NTP MYEL F+VPM+GA+LN IN RLDART+SVLL HC+SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDPE 180
             S L   A ++L      P LVLI + +   EE   + V     F   Y D++ +G+P+
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEE---EEEGGAEVTERSKFCYLYSDLITRGNPD 180

Query: 181 FKWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLPM 240
           FKW+RP S+WDPIV+NYTSGTTSSPKGVVHCHRGIF+++LDSL +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPD-NR 300
           FHANGW+YPWGIAA+GGTN+C+RK  A +I+ LIR H VTHM GAP+VL +L+ S + ++
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEER 360
           PL  PV  LTAG+ PPA VLLR E+LGF V HGYGLTETAG+++ C+WKP W+RLPA ++
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSM 420
           A+LK+RQGVRT   +  DVVDP +G+SV+ DG ++GEI LRG SIMLGYLK+P  T  S 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 TEEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVAR 480
            + GWF TGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNPAV+EAAVVAR
Sbjct: 421 -KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFVSLREGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQ 540
           PDEFWGETPCAFVSL+ GL R+PT++EI+EYC+ K+PR+M PKTV F  ELPKTSTGKI 
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KFVLRQMAKSM 549
           K +L+++AK+M
Sbjct: 541 KSLLKEIAKNM 544

BLAST of Sed0023845 vs. TAIR 10
Match: AT1G77240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 727.2 bits (1876), Expect = 9.6e-210
Identity = 348/553 (62.93%), Postives = 431/553 (77.94%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIYGEARYTWSETHRRCLQLASSLSSA- 60
           ME L P  +NS PLT LGFLERAA+++GD PS+++    +TWSETH RCL++AS+LSSA 
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 61  --IHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFVD 120
             I+RG VVSV+ PN P++YEL FAVPM+GAVLN IN RLDA  +SVLL H +SKLVFVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 121 EASSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGDP 180
             SSSL+  A S LP D +RP LV++ DG+      ++      +DF+D+YE  + +GD 
Sbjct: 121 HHSSSLVLEAVSFLPKD-ERPRLVILNDGNDMPSSSSAD-----MDFLDTYEGFMERGDL 180

Query: 181 EFKWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTLP 240
            FKWVRP S+W P+VLNYTSGTTSSPKGVVH HR +F+ +++SLL+W +P + VYLWTLP
Sbjct: 181 RFKWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLP 240

Query: 241 MFHANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDNR 300
           MFHANGWSY W  AA+G  NIC+ + D   IF+LI ++ VTHMC AP+VLNMLTN P  +
Sbjct: 241 MFHANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQK 300

Query: 301 PLDRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEER 360
           PL  PVK++TAGAPPPA V+ + EALGFDV HGYG+TET GLV+ C+ KP+WDRL  +ER
Sbjct: 301 PLQSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDER 360

Query: 361 ARLKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKSM 420
           A+ K+RQG+RTA  A  DV DP +GKSVK DG ++GEI  RG S+MLGY KDP  TA SM
Sbjct: 361 AKQKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASM 420

Query: 421 TEEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVAR 480
            E+GWF TGD+GVMHPDGYLE+KDRSKDV+I GGEN+SS E+E+VLYTNPA+ EAAVVA+
Sbjct: 421 REDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAK 480

Query: 481 PDEFWGETPCAFVSLR--EGLRRRPTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540
           PD+ WGETPCAFVSL+  +G     TE EI E+C+ KLP++MVP+ VVF  ELPKTSTGK
Sbjct: 481 PDKMWGETPCAFVSLKYHDG---SVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGK 540

Query: 541 IQKFVLRQMAKSM 549
           IQKF+LRQMAKS+
Sbjct: 541 IQKFLLRQMAKSL 544

BLAST of Sed0023845 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 707.2 bits (1824), Expect = 1.0e-203
Identity = 342/554 (61.73%), Postives = 427/554 (77.08%), Query Frame = 0

Query: 1   MEDLKPRPANSSPLTPLGFLERAAAIYGDCPSIIY-GEARYTWSETHRRCLQLASSLSSA 60
           ME L P P+NS+PLT LGFL+RAA++YGDCPSI++     +TWSETH RCL++AS+L+S+
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  ---IHRGDVVSVLSPNTPAMYELHFAVPMAGAVLNTINLRLDARTISVLLSHCDSKLVFV 120
              I+RG VVSV+ PN P++YEL FAVPM+GA+LN IN RLDA  +SVLL H +SKLVFV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DEASSSLIFHAFSLLPPDAKRPPLVLIRDGDAAAEEFTSSPVPITLDFVDSYEDMVRKGD 180
           D  S S++  A S +  + K P LVL+ D     E+ + SP   + DF+D+Y+ ++ +GD
Sbjct: 121 DPNSISVVLEAVSFMRQNEK-PHLVLLDDDQ---EDGSLSPSAAS-DFLDTYQGVMERGD 180

Query: 181 PEFKWVRPASDWDPIVLNYTSGTTSSPKGVVHCHRGIFLVSLDSLLEWGVPKQSVYLWTL 240
             FKW+RP ++W P++LNYTSGTTSSPKGVV  HR IF++++ SLL+W  P + VYLWTL
Sbjct: 181 SRFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTL 240

Query: 241 PMFHANGWSYPWGIAAIGGTNICIRKFDATAIFSLIRRHHVTHMCGAPVVLNMLTNSPDN 300
           PMFHANGW Y WG AA+G TN+C R+ DA  I+ LI +HHVTHMC AP+VLNMLTN P  
Sbjct: 241 PMFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSR 300

Query: 301 RPLDRPVKILTAGAPPPAAVLLRTEALGFDVCHGYGLTETAGLVICCSWKPQWDRLPAEE 360
           +PL  PV+++TAGAPPPAA++ R E LGF+V HGYGLTET G V+ C+WK +WD L   E
Sbjct: 301 KPLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLE 360

Query: 361 RARLKARQGVRTAAMAVADVVDPATGKSVKCDGVSIGEIALRGPSIMLGYLKDPAATAKS 420
           RARLK+RQGVRT   A  DV DP TGKSV+ DGVS+GEI L+G S+MLGY KDP  TA  
Sbjct: 361 RARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAAC 420

Query: 421 MTEEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVHEAAVVA 480
           M E+GWF +GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE+VLYTNP V EAAVVA
Sbjct: 421 MREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVA 480

Query: 481 RPDEFWGETPCAFVSLREGLRRR--PTEEEIMEYCRGKLPRFMVPKTVVFTAELPKTSTG 540
           +PD+ WGETPCAFVSL+         TE EI E+C+ +LP++MVP+ V+F  ELPKTSTG
Sbjct: 481 KPDKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTSTG 540

Query: 541 KIQKFVLRQMAKSM 549
           KIQKF+LRQMAKS+
Sbjct: 541 KIQKFLLRQMAKSL 549