Sed0023549 (gene) Chayote v1

BLAST of Sed0023549 vs. NCBI nr
Match: XP_022151211.1 (protein NRT1/ PTR FAMILY 2.13-like [Momordica charantia])

HSP 1 Score: 867.1 bits (2239), Expect = 8.2e-248
Identity = 443/575 (77.04%), Postives = 482/575 (83.83%), Query Frame = 0

Query: 15  SSSQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTATSVINIWSIAN 74
           SS QRKPGGWKTMPYILGNETFERLASTGLLANFMVYLR VYH+NQV ATS+IN+WS+AN
Sbjct: 27  SSPQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRRVYHLNQVAATSLINVWSMAN 86

Query: 75  NCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCNAPLEATDQCK 134
           N TPLVGAF+SDA+LGRF+TIAI S+ S+LGMVGMTFTAWIP LHPP+C+ PL+AT QCK
Sbjct: 87  NFTPLVGAFLSDAHLGRFYTIAIGSVASILGMVGMTFTAWIPQLHPPECSNPLQATSQCK 146

Query: 135 GPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSFYNLYYASFSI 194
           G T+SQ+GFLIMSL LLSIGSGGIRPCSVPFGVDQFDTT+E+GRKGLGSFYN YYA+FSI
Sbjct: 147 GATTSQMGFLIMSLSLLSIGSGGIRPCSVPFGVDQFDTTSEEGRKGLGSFYNWYYATFSI 206

Query: 195 ILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVGSVFTGLAQVL 254
           ILVFT TVVVYIQDSVSWVLGY IPT+LMFCAIVLFFLGTRVYVY+KP GSVFTGLA+V+
Sbjct: 207 ILVFTATVVVYIQDSVSWVLGYGIPTLLMFCAIVLFFLGTRVYVYMKPEGSVFTGLARVV 266

Query: 255 VATYKKRNLKLSSNGAID--QPTFYDPPLDSGTAVVSKLPLTIQY--------------- 314
           VATYKKR LKLSS  A D   PTF DPPLDSGTAV+ KLPLT QY               
Sbjct: 267 VATYKKRKLKLSSPDATDAKTPTFXDPPLDSGTAVIPKLPLTTQYRFLNKAAIVLENELK 326

Query: 315 ------------------------RVLPIWGSTILCLLTIVQQGTFSVSQALQMDRHMGP 374
                                   RV+PIW STILCL TI+QQGTFSVSQALQMDRH+GP
Sbjct: 327 PDGTPVNKWSLCSIQQVEELKCLVRVIPIWASTILCLTTIIQQGTFSVSQALQMDRHLGP 386

Query: 375 KFQVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGSVFAVMAMV 434
            F+VPAASI V SYLTL+IWI LYDRV VP+LRKIT HPNGITQLQRIGIGSVFAV+AMV
Sbjct: 387 SFKVPAASIGVTSYLTLAIWIPLYDRVIVPQLRKITNHPNGITQLQRIGIGSVFAVIAMV 446

Query: 435 IAGIIEKERRNMALRHNQKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFPEHLRTMAN 494
           +AGI+EKERR MA RH Q+MSVFWL PQH LMGLCEAFSIIGQ+EFFNNEFPE LRTMAN
Sbjct: 447 VAGIVEKERREMAHRH-QQMSVFWLAPQHILMGLCEAFSIIGQMEFFNNEFPEQLRTMAN 506

Query: 495 ALLSISFTIAVYVSTLVIIIVHKTTGGHGKPDWLTNDLNDGRVDYFYFIVAGIAFFNLFL 549
           ALLSISFT A YVSTLV+IIVHKTTGGHGKPDWLTNDLN GRVDY+Y+IVA IAF N+FL
Sbjct: 507 ALLSISFTTATYVSTLVVIIVHKTTGGHGKPDWLTNDLNAGRVDYYYYIVAAIAFVNIFL 566

BLAST of Sed0023549 vs. NCBI nr
Match: KAG6601403.1 (Protein NRT1/ PTR FAMILY 2.13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 692.2 bits (1785), Expect = 3.6e-195
Identity = 353/584 (60.45%), Postives = 432/584 (73.97%), Query Frame = 0

Query: 10  SNAFVSSSQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTATSVINI 69
           +N  +  S+R+PGGWK+MPYILGNETFERLAS GLLANFMVYLR VYHM+QV+ATS++ +
Sbjct: 9   ANPKLRKSRREPGGWKSMPYILGNETFERLASMGLLANFMVYLRKVYHMDQVSATSLMGL 68

Query: 70  WSIANNCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCNAPLEA 129
           W+   N  PL+GAF+SDAY GR++TIA+AS  S LGM  MT TAW+P LHPP C+     
Sbjct: 69  WTGVTNFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPTCSGA--- 128

Query: 130 TDQCKGPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSFYNLYY 189
              C+GPT+SQLGFL+MSLCL+SIGS GIRPCS+PFGVDQFD TT+KGRKG+ SFYN YY
Sbjct: 129 --NCEGPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYNWYY 188

Query: 190 ASFSIILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVGSVFTG 249
           A+FS++LVFT T+VVYIQDSVSWVLGY IPTVLMFC+IVLFF+GT VYV++KP GS+FTG
Sbjct: 189 ATFSVVLVFTLTIVVYIQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSIFTG 248

Query: 250 LAQVLVATYKKRNLKLSSNGAIDQPTFYDPPLDSGTAVVSKLPLTIQY------------ 309
           LAQV VA YKKR+L+L S    D+  FYDPPL+ G+ V+SKLPLT Q+            
Sbjct: 249 LAQVAVAAYKKRHLELPS--LDDRTAFYDPPLEPGSVVLSKLPLTNQFSFLNKAAIVVEK 308

Query: 310 ---------------------------RVLPIWGSTILCLLTIVQQGTFSVSQALQMDRH 369
                                      RV PIW + IL L  I+QQ TFS+SQALQMDRH
Sbjct: 309 ELNPDGTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQMDRH 368

Query: 370 MGPKFQVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGSVFAVM 429
           MGP FQ+P ASI VIS+LT++ +I LYD+  VP  RK T HPNGIT+LQR+ IG +FAV+
Sbjct: 369 MGPNFQMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTHHPNGITELQRMAIGIIFAVL 428

Query: 430 AMVIAGIIEKERRNMALRHN--QKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFPEHL 489
           +M++AGI+EKERRN A   N    MSVFWL PQ  LMGLCEAF+IIGQ+EFFN EFPEH+
Sbjct: 429 SMIVAGIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHM 488

Query: 490 RTMANALLSISFTIAVYVSTLVIIIVHKTT---GGHGKPDWLTNDLNDGRVDYFYFIVAG 549
           RTM NA  S S  ++ YV+T +++IVH+TT   G HGKPDWL NDLN GR+DYFY++VA 
Sbjct: 489 RTMGNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLANDLNKGRLDYFYYVVAA 548

BLAST of Sed0023549 vs. NCBI nr
Match: XP_022957160.1 (protein NRT1/ PTR FAMILY 2.13-like [Cucurbita moschata])

HSP 1 Score: 692.2 bits (1785), Expect = 3.6e-195
Identity = 352/584 (60.27%), Postives = 432/584 (73.97%), Query Frame = 0

Query: 10  SNAFVSSSQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTATSVINI 69
           +N  +  S+R+PGGWK+MPYILGNETFERLAS GLLANFMVYLR VYHM+QV+ATS++ +
Sbjct: 9   ANPKLRKSRREPGGWKSMPYILGNETFERLASMGLLANFMVYLRKVYHMDQVSATSLMGL 68

Query: 70  WSIANNCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCNAPLEA 129
           W+   N  PL+GAF+SDAY GR++TIA+AS  S LGM  MT TAW+P LHPP C+     
Sbjct: 69  WTGVTNFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPTCSGA--- 128

Query: 130 TDQCKGPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSFYNLYY 189
              C+GPT+SQLGFL+MSLCL+SIGS GIRPCS+PFGVDQFD TT+KGRKG+ SFYN YY
Sbjct: 129 --NCEGPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYNWYY 188

Query: 190 ASFSIILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVGSVFTG 249
           A+FS++LVFT T+VVYIQDSVSWVLGY IPTVLMFC+IVLFF+GT VYV++KP GS+FTG
Sbjct: 189 ATFSVVLVFTLTIVVYIQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSIFTG 248

Query: 250 LAQVLVATYKKRNLKLSSNGAIDQPTFYDPPLDSGTAVVSKLPLTIQY------------ 309
           LAQV VA YKKR+L+L S    D+  FYDPPL+ G+ V+SKLPLT Q+            
Sbjct: 249 LAQVAVAAYKKRHLELPS--LDDRTAFYDPPLEPGSVVLSKLPLTNQFSFLNKAAIVVEK 308

Query: 310 ---------------------------RVLPIWGSTILCLLTIVQQGTFSVSQALQMDRH 369
                                      RV PIW + IL L  I+QQ TFS+SQALQMDRH
Sbjct: 309 ELNPDGTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQMDRH 368

Query: 370 MGPKFQVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGSVFAVM 429
           MGP FQ+P ASI VIS+LT++ +I LYD+  VP  RK T HPNGIT+LQR+ +G +FAV+
Sbjct: 369 MGPNFQMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTHHPNGITELQRMAVGIIFAVL 428

Query: 430 AMVIAGIIEKERRNMALRHN--QKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFPEHL 489
           +M++AGI+EKERRN A   N    MSVFWL PQ  LMGLCEAF+IIGQ+EFFN EFPEH+
Sbjct: 429 SMIVAGIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHM 488

Query: 490 RTMANALLSISFTIAVYVSTLVIIIVHKTT---GGHGKPDWLTNDLNDGRVDYFYFIVAG 549
           RTM NA  S S  ++ YV+T +++IVH+TT   G HGKPDWL NDLN GR+DYFY++VA 
Sbjct: 489 RTMGNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLANDLNKGRLDYFYYVVAA 548

BLAST of Sed0023549 vs. NCBI nr
Match: KAG7032183.1 (Protein NRT1/ PTR FAMILY 2.13 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 691.8 bits (1784), Expect = 4.7e-195
Identity = 352/584 (60.27%), Postives = 432/584 (73.97%), Query Frame = 0

Query: 10  SNAFVSSSQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTATSVINI 69
           +N  +  S+R+PGGWK+MPYILGNETFERLAS GLLANFMVYLR VYHM+QV+ATS++ +
Sbjct: 9   ANPKLRKSRREPGGWKSMPYILGNETFERLASMGLLANFMVYLRKVYHMDQVSATSLMGL 68

Query: 70  WSIANNCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCNAPLEA 129
           W+   N  PL+GAF+SDAY GR++TIA+AS  S LGM  MT TAW+P LHPP C+     
Sbjct: 69  WTGVTNFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPTCSGA--- 128

Query: 130 TDQCKGPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSFYNLYY 189
              C+GPT+SQLGFL+MSLCL+SIGS GIRPCS+PFGVDQFD TT+KGRKG+ SFYN YY
Sbjct: 129 --NCEGPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYNWYY 188

Query: 190 ASFSIILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVGSVFTG 249
           A+FS++LVFT T+VVY+QDSVSWVLGY IPTVLMFC+IVLFF+GT VYV++KP GS+FTG
Sbjct: 189 ATFSVVLVFTLTIVVYVQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSIFTG 248

Query: 250 LAQVLVATYKKRNLKLSSNGAIDQPTFYDPPLDSGTAVVSKLPLTIQY------------ 309
           LAQV VA YKKR+L+L S    D+  FYDPPL+ G+ V+SKLPLT Q+            
Sbjct: 249 LAQVAVAAYKKRHLELPS--LDDRTAFYDPPLEPGSVVLSKLPLTNQFSFLNKAAIVVEK 308

Query: 310 ---------------------------RVLPIWGSTILCLLTIVQQGTFSVSQALQMDRH 369
                                      RV PIW + IL L  I+QQ TFS+SQALQMDRH
Sbjct: 309 ELNPDGTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQMDRH 368

Query: 370 MGPKFQVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGSVFAVM 429
           MGP FQ+P ASI VIS+LT++ +I LYD+  VP  RK T HPNGIT+LQR+ IG +FAV+
Sbjct: 369 MGPNFQMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTHHPNGITELQRMAIGIIFAVL 428

Query: 430 AMVIAGIIEKERRNMALRHN--QKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFPEHL 489
           +M++AGI+EKERRN A   N    MSVFWL PQ  LMGLCEAF+IIGQ+EFFN EFPEH+
Sbjct: 429 SMIVAGIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHM 488

Query: 490 RTMANALLSISFTIAVYVSTLVIIIVHKTT---GGHGKPDWLTNDLNDGRVDYFYFIVAG 549
           RTM NA  S S  ++ YV+T +++IVH+TT   G HGKPDWL NDLN GR+DYFY++VA 
Sbjct: 489 RTMGNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLANDLNKGRLDYFYYVVAA 548

BLAST of Sed0023549 vs. NCBI nr
Match: XP_022998476.1 (protein NRT1/ PTR FAMILY 2.13-like [Cucurbita maxima])

HSP 1 Score: 688.0 bits (1774), Expect = 6.9e-194
Identity = 349/579 (60.28%), Postives = 430/579 (74.27%), Query Frame = 0

Query: 15  SSSQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTATSVINIWSIAN 74
           + ++R+PGGWK+MPYILGNETFERLAS GLLANFMVYL  VYHM+QV+ATS++ +W+   
Sbjct: 19  NKAKREPGGWKSMPYILGNETFERLASMGLLANFMVYLTKVYHMDQVSATSLMGLWTGVT 78

Query: 75  NCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCNAPLEATDQCK 134
           N  PL+GAF+SDAY GR++TIA+AS  S LGM  MT TAW+P LHPP C+        C+
Sbjct: 79  NFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPTCSGA-----NCE 138

Query: 135 GPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSFYNLYYASFSI 194
           GPT+SQLGFL+MSLCL+SIGS GIRPCS+PFGVDQFD TT+KGRKG+ SFYN YYA+FS+
Sbjct: 139 GPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYNWYYATFSV 198

Query: 195 ILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVGSVFTGLAQVL 254
           +LVFT T+VVYIQDSVSWVLGY IPTVLMFC+IVLFF+GT VYV++KP GS+FTGLAQV 
Sbjct: 199 VLVFTLTIVVYIQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSIFTGLAQVA 258

Query: 255 VATYKKRNLKLSSNGAIDQPTFYDPPLDSGTAVVSKLPLTIQY----------------- 314
           VA YKKR+L+L S    D+  FYDPPL+  + V+SKLPLT Q+                 
Sbjct: 259 VAAYKKRHLELPS--LDDRTAFYDPPLEPASVVLSKLPLTNQFSFLTKAAIVVEKELNPD 318

Query: 315 ----------------------RVLPIWGSTILCLLTIVQQGTFSVSQALQMDRHMGPKF 374
                                 RV PIW + IL L  I+QQ TFS+SQALQMDRHMGP F
Sbjct: 319 GTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQMDRHMGPNF 378

Query: 375 QVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGSVFAVMAMVIA 434
           Q+P ASI VIS+LT++ +I LYD+  VP  RK T+HPNGIT+LQR+ +G +FAV++M++A
Sbjct: 379 QMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTQHPNGITELQRMAVGIIFAVLSMIVA 438

Query: 435 GIIEKERRNMALRHN--QKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFPEHLRTMAN 494
           GI+EKERRN A   N    MSVFWL PQ  LMGLCEAF+IIGQ+EFFN EFPEH+RTM N
Sbjct: 439 GIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRTMGN 498

Query: 495 ALLSISFTIAVYVSTLVIIIVHKTT---GGHGKPDWLTNDLNDGRVDYFYFIVAGIAFFN 549
           A  S S  ++ YV+T +++IVH+TT   G HGKPDWLTNDLN GR+DYFY++VA  AFFN
Sbjct: 499 AFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLTNDLNKGRLDYFYYVVAATAFFN 558

BLAST of Sed0023549 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 1.6e-161
Identity = 298/596 (50.00%), Postives = 406/596 (68.12%), Query Frame = 0

Query: 7   NPTSNAFVSSSQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTATSV 66
           +P S       ++KPGGW+ + +ILGNET ERL S GLLANFMVYL  V+H+ QV A +V
Sbjct: 38  SPGSILDAEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANV 97

Query: 67  INIWSIANNCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCNA- 126
           INIWS   N TPLVGA+ISD Y+GRF TIA AS  +LLG++ +T TA  P LHP  CN+ 
Sbjct: 98  INIWSGFTNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQ 157

Query: 127 -PLEATDQCKGPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSF 186
            PL     C GP   Q+G L++ LC LS+GSGGIRPCS+PFGVDQFD  TE+G KG+ SF
Sbjct: 158 DPL----SCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASF 217

Query: 187 YNLYYASFSIILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVG 246
           +N YY +F+++L+ T TVVVYIQD VSW++G++IPT LM  A+V+FF G + YVY+KP G
Sbjct: 218 FNWYYMTFTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEG 277

Query: 247 SVFTGLAQVLVATYKKRNLKL--SSNGAIDQPTFYDPPLDSGTAVVSKLPLTIQY----- 306
           S+F+G+AQV+VA  KKR LKL    +G +   T+YDP + S  +V+SKL  + Q+     
Sbjct: 278 SIFSGIAQVIVAARKKRKLKLPAEDDGTV---TYYDPAIKS--SVLSKLHRSNQFRCLDK 337

Query: 307 ----------------------------------RVLPIWGSTILCLLTIVQQGTFSVSQ 366
                                             R++PIW + I+ L  +  QGTF+VSQ
Sbjct: 338 AAVVIEGDLTPEGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQ 397

Query: 367 ALQMDRHMGPKFQVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGI 426
           AL+MDR++GPKF++PA S++VIS LT+ I++  YDRVFVP +R+IT H +GIT LQRIG 
Sbjct: 398 ALKMDRNLGPKFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGT 457

Query: 427 GSVFAVMAMVIAGIIEKERRNMALRHNQ-----KMSVFWLVPQHSLMGLCEAFSIIGQLE 486
           G VFA+ +M++AGI+E+ RR  ++          MSVFWL PQ  LMGLCEAF+IIGQ+E
Sbjct: 458 GIVFAIFSMIVAGIVERMRRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIE 517

Query: 487 FFNNEFPEHLRTMANALLSISFTIAVYVSTLVIIIVHKTTGGHGKPDWLTNDLNDGRVDY 546
           FFN++FPEH+R++AN+L S+SF  + Y+S+ ++ +VHK +GGH +PDWL  +LN G++DY
Sbjct: 518 FFNSQFPEHMRSIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDY 577

BLAST of Sed0023549 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 515.8 bits (1327), Expect = 6.1e-145
Identity = 270/548 (49.27%), Postives = 369/548 (67.34%), Query Frame = 0

Query: 18  QRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTATSVINIWSIANNCT 77
           ++K GGW+ + +ILGNET E+L S G+ ANFM+YLR+V+HM  V A +V  +W    N  
Sbjct: 12  EKKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFA 71

Query: 78  PLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCNAPLEATDQCKGPT 137
           PL+GA ISDAY+GRF TIA AS+ S+LG++ +T TA +P LHPP CN P    D+C  P 
Sbjct: 72  PLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNP--HPDECDDPN 131

Query: 138 SSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSFYNLYYASFSIILV 197
             QLG L + L  LSIGSGGIRPCS+PFGVDQFD  TE+G KG+ SF+N YY + +++L+
Sbjct: 132 KLQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLI 191

Query: 198 FTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVGSVFTGLAQVLVAT 257
           F+ TVVVY+Q +VSWV+G++IPT LM CA+VLFF+G R YVY+KP GSVF+G+A+V+VA 
Sbjct: 192 FSHTVVVYLQ-TVSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAA 251

Query: 258 YKKRNLKLSSNGAIDQPT--FYDPPLDSGTAVVSKLPLTIQY------------------ 317
            KKR+LK+S    +D  T  +Y+PP+  G  V+SKLPLT Q+                  
Sbjct: 252 RKKRDLKIS---LVDDGTEEYYEPPVKPG--VLSKLPLTDQFKFLDKAAVILDGDLTSEG 311

Query: 318 ---------------------RVLPIWGSTILCLLTIVQQGTFSVSQALQMDRHMGPKFQ 377
                                RV+P+W + I+ ++ +  Q TF V QA +MDRHMGP F+
Sbjct: 312 VPANKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFE 371

Query: 378 VPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGSVFAVMAMVIAG 437
           +PAASI VISY+T+ IW+ +Y+ + VP L ++ K    +T LQR+GIG VFA+++M  AG
Sbjct: 372 IPAASITVISYITIGIWVPIYEHLLVPFLWRMRKF--RVTLLQRMGIGIVFAILSMFTAG 431

Query: 438 IIEKERRNMALRHNQKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFPEHLRTMANALL 497
            +E  RR  A    Q MSVFWL     LMGLCE+F+ IG +EFFN++FPEH+R++AN+L 
Sbjct: 432 FVEGVRRTRATEMTQ-MSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLF 491

Query: 498 SISFTIAVYVSTLVIIIVHKTTGGHGKPDWLTNDLNDGRVDYFYFIVAGIAFFNLFLFVY 525
            +SF  A Y+S+L++  VHK +G    PDWL  DL+ G++DYFY+++A +   NL  F Y
Sbjct: 492 PLSFAAANYLSSLLVTTVHKVSGTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVNLVYFWY 548

BLAST of Sed0023549 vs. ExPASy Swiss-Prot
Match: Q9CAR9 (Putative protein NRT1/ PTR FAMILY 2.14 OS=Arabidopsis thaliana OX=3702 GN=NPF2.14 PE=5 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 6.6e-131
Identity = 250/556 (44.96%), Postives = 365/556 (65.65%), Query Frame = 0

Query: 5   NENPTSNAFVSSSQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTAT 64
           NE  TS++ +++ QRKP GWK MPYI+GNET ERLA+ GL+ANFMVY+   YHM+QV A 
Sbjct: 3   NEKGTSSSDLTTRQRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQAV 62

Query: 65  SVINIWSIANNCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCN 124
           ++IN WS   N  P++GAFISD+Y G+F TI   SI  LLGM+ +TFT+ +P+L PP C 
Sbjct: 63  TLINTWSALTNFAPIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPCT 122

Query: 125 APLEATDQCKGPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSF 184
           A  + T QC   + SQL  L+  L LLS+G+GGIR CSVPF +DQFD +TE+GR+G  SF
Sbjct: 123 AD-QITGQCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSF 182

Query: 185 YNLYYASFSIILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVG 244
           ++ YY + +I+ + + T+V+Y+Q+++SW +G+AIPTVL F A++L F+GTR YV++KP G
Sbjct: 183 FSWYYTTHTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEG 242

Query: 245 SVFTGLAQVLVATYKKRNLKLSSNGAIDQPTFYDPPLDSGTAVVSKLPLTIQYR------ 304
           SVF+G+ +VLVA YKKR  + +S   ID    Y  PL       +KL LT Q+R      
Sbjct: 243 SVFSGVFKVLVAAYKKRKARFTS--GID----YHQPLLETDLQSNKLVLTDQFRFLNKAV 302

Query: 305 -----------------------------VLPIWGSTILCLLTIVQQGTFSVSQALQMD- 364
                                        ++PI+ S+I+  L + QQ TF+VSQAL+MD 
Sbjct: 303 IVMNNDEAGNEEWRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDL 362

Query: 365 RHMGPKFQVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGSVFA 424
           +  G  + +P ASI VIS L + IW+  Y+ V V  +  ITK   GI+ LQ++GIG++F+
Sbjct: 363 QFPGTSYLIPPASITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFS 422

Query: 425 VMAMVIAGIIEKERRNMALRHNQKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFPEHL 484
           +  M+I+GI+E++RR+++L +  KMSVFWL PQ  LMG  + F+I+G  EFFN + P ++
Sbjct: 423 ISTMLISGIVERKRRDLSL-NGVKMSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINM 482

Query: 485 RTMANALLSISFTIAVYVSTLVIIIVHKTTGGHGKPDWLTNDLNDGRVDYFYFIVAGIAF 525
           R++ N+LL +  ++A Y+S+ ++ IVH  T   G   WLT+D++  ++D FY+ +A ++ 
Sbjct: 483 RSIGNSLLYLGLSLASYLSSAMVSIVHSVT-ARGGQSWLTDDIDKSKLDCFYYFIAALST 542

BLAST of Sed0023549 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 1.3e-110
Identity = 218/560 (38.93%), Postives = 322/560 (57.50%), Query Frame = 0

Query: 23  GWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTATSVINIWSIANNCTPLVGA 82
           GWK MP+I+GNETFE+L   G L+N +VYL +V+++  +TA ++IN +S   N    V A
Sbjct: 46  GWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTINFGTFVAA 105

Query: 83  FISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCNAPLEATDQCKGPTSSQLG 142
           F+ D Y GR+ T+++A I   LG   +  TA +P LHP  C    ++   C GP+  Q+ 
Sbjct: 106 FLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSI--CNGPSGGQIA 165

Query: 143 FLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSFYNLYYASFSIILVFTGTV 202
           FL+M L  L +G+GGIRPC++ FG DQF+  +E G++G+ SF+N Y+ +F+   + + T+
Sbjct: 166 FLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQILSLTL 225

Query: 203 VVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVGSVFTGLAQVLVATYKKRN 262
           VVY+Q +VSW +G  IP VLMF A ++FF G ++YV +K  GS   G+AQV+    KKR 
Sbjct: 226 VVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIKKRG 285

Query: 263 LKLSSNGAIDQPTFYDPPLDSGTAVVSKLPLTIQY------------------------- 322
           LK +    ++   +Y P   +     SKL  T Q+                         
Sbjct: 286 LKPAKQPWLNLYNYYPPKYAN-----SKLKYTDQFRFLDKAAILTPEDKLQPDGKPADPW 345

Query: 323 ---------------RVLPIWGSTILCLLTIVQQGTFSVSQALQMDRHMGP-KFQVPAAS 382
                          RVLPIW ++ +  LTI QQ T+ V QALQ DR +G   F +PAA+
Sbjct: 346 KLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAAT 405

Query: 383 IAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGSVFAVMAMVIAGIIEKE 442
             V     ++++I++YDRV VP +R+IT    GIT LQRIG G  FA  ++V+AG +E+ 
Sbjct: 406 YVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEER 465

Query: 443 RRNMALR-----------HNQKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFPEHLRT 502
           RR  AL                MS  WL+PQ SL G+ EAF+ IGQ+EF+  +FPE++R+
Sbjct: 466 RRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMRS 525

Query: 503 MANALLSISFTIAVYVSTLVIIIVHKTTGGHGKPDWLTNDLNDGRVDYFYFIVAGIAFFN 531
            A ++  +   ++ Y+ + +I  VH+TT      +WL  DLN GR+D FYF++AGI   N
Sbjct: 526 FAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWLAEDLNKGRLDLFYFMIAGILAVN 585

BLAST of Sed0023549 vs. ExPASy Swiss-Prot
Match: Q944G5 (Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana OX=3702 GN=NPF2.10 PE=1 SV=3)

HSP 1 Score: 395.2 bits (1014), Expect = 1.2e-108
Identity = 231/597 (38.69%), Postives = 340/597 (56.95%), Query Frame = 0

Query: 1   METQNENPTSNAFVSSS----QRK--PGGWKTMPYILGNETFERLASTGLLANFMVYLRS 60
           +E +    T+N  V  S    QRK    GWK MP+I+GNETFE+L   G L+N +VYL S
Sbjct: 36  LEVEPSTTTTNTDVVDSFEEEQRKIVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTS 95

Query: 61  VYHMNQVTATSVINIWSIANNCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAW 120
           V+++   TA ++IN +S   N    + AF+ D Y GR+ T+++A I   LG   +  TA 
Sbjct: 96  VFNLKSYTAATIINAFSGTINFGTFIAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAA 155

Query: 121 IPHLHPPKCNAPLEATDQCKGPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTT 180
           IP LHP  C   +     C+GP+  Q+ FL+M L  L +G+GGIRPC++ FG DQF+  +
Sbjct: 156 IPSLHPVACGNKI----SCEGPSVGQILFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKS 215

Query: 181 EKGRKGLGSFYNLYYASFSIILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGT 240
           E G+KG+ SF+N Y+ +F+   + + T VVYIQ +VSW +G  IP  LMF A V+FF G 
Sbjct: 216 ESGKKGINSFFNWYFFTFTFAQIISLTAVVYIQSNVSWTIGLIIPVALMFLACVIFFAGD 275

Query: 241 RVYVYLKPVGSVFTGLAQVLVATYKKRNLK------------LSSNGA------IDQPTF 300
           R+YV +K  GS   G+A+V+ A  KKR LK            + SN A       DQ  F
Sbjct: 276 RLYVKVKASGSPLAGIARVIAAAIKKRGLKPVKQPWVNLYNHIPSNYANTTLKYTDQFRF 335

Query: 301 YDPPL---------DSGTAVVSKLPLTIQ--------YRVLPIWGSTILCLLTIVQQGTF 360
            D              GTA       T+Q         RV+PIW ++ +  L I  Q T+
Sbjct: 336 LDKAAIMTPEEKLNSDGTASDPWKLCTLQQVEEVKCIVRVIPIWFASTIYYLAITIQMTY 395

Query: 361 SVSQALQMDRHMGP-KFQVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQL 420
            V QALQ DR +G   F++PAA+  V     ++++I+ YDRV VP LR++T    GI+ L
Sbjct: 396 PVFQALQSDRRLGSGGFRIPAATYVVFLMTGMTVFIIFYDRVLVPSLRRVTGLETGISLL 455

Query: 421 QRIGIGSVFAVMAMVIAGIIEKERRNMALR-----------HNQKMSVFWLVPQHSLMGL 480
           QRIG G  FA+M+++++G IE+ RRN AL                MS  WL+PQ +L G+
Sbjct: 456 QRIGAGFTFAIMSLLVSGFIEERRRNFALTKPTLGMAPRTGEISSMSALWLIPQLTLAGI 515

Query: 481 CEAFSIIGQLEFFNNEFPEHLRTMANALLSISFTIAVYVSTLVIIIVHKTTGGHGKPDWL 540
            EAF+ IGQ+EF+  +FPE++++ A ++  +   ++ Y+++ +I  VH+TT      +WL
Sbjct: 516 AEAFAAIGQMEFYYKQFPENMKSFAGSIFYVGAGVSSYLASFLISTVHRTTAHSPSGNWL 575

Query: 541 TNDLNDGRVDYFYFIVAGIAFFNLFLFVYCARSYRYKGTGENLGVKPMDEVELSSEK 545
             DLN  ++DYFYF++ G+   N+  F+  AR YRYKG  +    + + E+E + E+
Sbjct: 576 AEDLNKAKLDYFYFMLTGLMVVNMAYFLLMARWYRYKGGND----EDITEIETNEEE 624

BLAST of Sed0023549 vs. ExPASy TrEMBL
Match: A0A6J1DDX4 (protein NRT1/ PTR FAMILY 2.13-like OS=Momordica charantia OX=3673 GN=LOC111019188 PE=3 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 4.0e-248
Identity = 443/575 (77.04%), Postives = 482/575 (83.83%), Query Frame = 0

Query: 15  SSSQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTATSVINIWSIAN 74
           SS QRKPGGWKTMPYILGNETFERLASTGLLANFMVYLR VYH+NQV ATS+IN+WS+AN
Sbjct: 27  SSPQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRRVYHLNQVAATSLINVWSMAN 86

Query: 75  NCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCNAPLEATDQCK 134
           N TPLVGAF+SDA+LGRF+TIAI S+ S+LGMVGMTFTAWIP LHPP+C+ PL+AT QCK
Sbjct: 87  NFTPLVGAFLSDAHLGRFYTIAIGSVASILGMVGMTFTAWIPQLHPPECSNPLQATSQCK 146

Query: 135 GPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSFYNLYYASFSI 194
           G T+SQ+GFLIMSL LLSIGSGGIRPCSVPFGVDQFDTT+E+GRKGLGSFYN YYA+FSI
Sbjct: 147 GATTSQMGFLIMSLSLLSIGSGGIRPCSVPFGVDQFDTTSEEGRKGLGSFYNWYYATFSI 206

Query: 195 ILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVGSVFTGLAQVL 254
           ILVFT TVVVYIQDSVSWVLGY IPT+LMFCAIVLFFLGTRVYVY+KP GSVFTGLA+V+
Sbjct: 207 ILVFTATVVVYIQDSVSWVLGYGIPTLLMFCAIVLFFLGTRVYVYMKPEGSVFTGLARVV 266

Query: 255 VATYKKRNLKLSSNGAID--QPTFYDPPLDSGTAVVSKLPLTIQY--------------- 314
           VATYKKR LKLSS  A D   PTF DPPLDSGTAV+ KLPLT QY               
Sbjct: 267 VATYKKRKLKLSSPDATDAKTPTFXDPPLDSGTAVIPKLPLTTQYRFLNKAAIVLENELK 326

Query: 315 ------------------------RVLPIWGSTILCLLTIVQQGTFSVSQALQMDRHMGP 374
                                   RV+PIW STILCL TI+QQGTFSVSQALQMDRH+GP
Sbjct: 327 PDGTPVNKWSLCSIQQVEELKCLVRVIPIWASTILCLTTIIQQGTFSVSQALQMDRHLGP 386

Query: 375 KFQVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGSVFAVMAMV 434
            F+VPAASI V SYLTL+IWI LYDRV VP+LRKIT HPNGITQLQRIGIGSVFAV+AMV
Sbjct: 387 SFKVPAASIGVTSYLTLAIWIPLYDRVIVPQLRKITNHPNGITQLQRIGIGSVFAVIAMV 446

Query: 435 IAGIIEKERRNMALRHNQKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFPEHLRTMAN 494
           +AGI+EKERR MA RH Q+MSVFWL PQH LMGLCEAFSIIGQ+EFFNNEFPE LRTMAN
Sbjct: 447 VAGIVEKERREMAHRH-QQMSVFWLAPQHILMGLCEAFSIIGQMEFFNNEFPEQLRTMAN 506

Query: 495 ALLSISFTIAVYVSTLVIIIVHKTTGGHGKPDWLTNDLNDGRVDYFYFIVAGIAFFNLFL 549
           ALLSISFT A YVSTLV+IIVHKTTGGHGKPDWLTNDLN GRVDY+Y+IVA IAF N+FL
Sbjct: 507 ALLSISFTTATYVSTLVVIIVHKTTGGHGKPDWLTNDLNAGRVDYYYYIVAAIAFVNIFL 566

BLAST of Sed0023549 vs. ExPASy TrEMBL
Match: A0A6J1GZR8 (protein NRT1/ PTR FAMILY 2.13-like OS=Cucurbita moschata OX=3662 GN=LOC111458629 PE=3 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.8e-195
Identity = 352/584 (60.27%), Postives = 432/584 (73.97%), Query Frame = 0

Query: 10  SNAFVSSSQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTATSVINI 69
           +N  +  S+R+PGGWK+MPYILGNETFERLAS GLLANFMVYLR VYHM+QV+ATS++ +
Sbjct: 9   ANPKLRKSRREPGGWKSMPYILGNETFERLASMGLLANFMVYLRKVYHMDQVSATSLMGL 68

Query: 70  WSIANNCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCNAPLEA 129
           W+   N  PL+GAF+SDAY GR++TIA+AS  S LGM  MT TAW+P LHPP C+     
Sbjct: 69  WTGVTNFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPTCSGA--- 128

Query: 130 TDQCKGPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSFYNLYY 189
              C+GPT+SQLGFL+MSLCL+SIGS GIRPCS+PFGVDQFD TT+KGRKG+ SFYN YY
Sbjct: 129 --NCEGPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYNWYY 188

Query: 190 ASFSIILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVGSVFTG 249
           A+FS++LVFT T+VVYIQDSVSWVLGY IPTVLMFC+IVLFF+GT VYV++KP GS+FTG
Sbjct: 189 ATFSVVLVFTLTIVVYIQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSIFTG 248

Query: 250 LAQVLVATYKKRNLKLSSNGAIDQPTFYDPPLDSGTAVVSKLPLTIQY------------ 309
           LAQV VA YKKR+L+L S    D+  FYDPPL+ G+ V+SKLPLT Q+            
Sbjct: 249 LAQVAVAAYKKRHLELPS--LDDRTAFYDPPLEPGSVVLSKLPLTNQFSFLNKAAIVVEK 308

Query: 310 ---------------------------RVLPIWGSTILCLLTIVQQGTFSVSQALQMDRH 369
                                      RV PIW + IL L  I+QQ TFS+SQALQMDRH
Sbjct: 309 ELNPDGTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQMDRH 368

Query: 370 MGPKFQVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGSVFAVM 429
           MGP FQ+P ASI VIS+LT++ +I LYD+  VP  RK T HPNGIT+LQR+ +G +FAV+
Sbjct: 369 MGPNFQMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTHHPNGITELQRMAVGIIFAVL 428

Query: 430 AMVIAGIIEKERRNMALRHN--QKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFPEHL 489
           +M++AGI+EKERRN A   N    MSVFWL PQ  LMGLCEAF+IIGQ+EFFN EFPEH+
Sbjct: 429 SMIVAGIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHM 488

Query: 490 RTMANALLSISFTIAVYVSTLVIIIVHKTT---GGHGKPDWLTNDLNDGRVDYFYFIVAG 549
           RTM NA  S S  ++ YV+T +++IVH+TT   G HGKPDWL NDLN GR+DYFY++VA 
Sbjct: 489 RTMGNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLANDLNKGRLDYFYYVVAA 548

BLAST of Sed0023549 vs. ExPASy TrEMBL
Match: A0A6J1KAA8 (protein NRT1/ PTR FAMILY 2.13-like OS=Cucurbita maxima OX=3661 GN=LOC111493070 PE=3 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 3.3e-194
Identity = 349/579 (60.28%), Postives = 430/579 (74.27%), Query Frame = 0

Query: 15  SSSQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTATSVINIWSIAN 74
           + ++R+PGGWK+MPYILGNETFERLAS GLLANFMVYL  VYHM+QV+ATS++ +W+   
Sbjct: 19  NKAKREPGGWKSMPYILGNETFERLASMGLLANFMVYLTKVYHMDQVSATSLMGLWTGVT 78

Query: 75  NCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCNAPLEATDQCK 134
           N  PL+GAF+SDAY GR++TIA+AS  S LGM  MT TAW+P LHPP C+        C+
Sbjct: 79  NFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPTCSGA-----NCE 138

Query: 135 GPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSFYNLYYASFSI 194
           GPT+SQLGFL+MSLCL+SIGS GIRPCS+PFGVDQFD TT+KGRKG+ SFYN YYA+FS+
Sbjct: 139 GPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYNWYYATFSV 198

Query: 195 ILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVGSVFTGLAQVL 254
           +LVFT T+VVYIQDSVSWVLGY IPTVLMFC+IVLFF+GT VYV++KP GS+FTGLAQV 
Sbjct: 199 VLVFTLTIVVYIQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSIFTGLAQVA 258

Query: 255 VATYKKRNLKLSSNGAIDQPTFYDPPLDSGTAVVSKLPLTIQY----------------- 314
           VA YKKR+L+L S    D+  FYDPPL+  + V+SKLPLT Q+                 
Sbjct: 259 VAAYKKRHLELPS--LDDRTAFYDPPLEPASVVLSKLPLTNQFSFLTKAAIVVEKELNPD 318

Query: 315 ----------------------RVLPIWGSTILCLLTIVQQGTFSVSQALQMDRHMGPKF 374
                                 RV PIW + IL L  I+QQ TFS+SQALQMDRHMGP F
Sbjct: 319 GTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQMDRHMGPNF 378

Query: 375 QVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGSVFAVMAMVIA 434
           Q+P ASI VIS+LT++ +I LYD+  VP  RK T+HPNGIT+LQR+ +G +FAV++M++A
Sbjct: 379 QMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTQHPNGITELQRMAVGIIFAVLSMIVA 438

Query: 435 GIIEKERRNMALRHN--QKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFPEHLRTMAN 494
           GI+EKERRN A   N    MSVFWL PQ  LMGLCEAF+IIGQ+EFFN EFPEH+RTM N
Sbjct: 439 GIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRTMGN 498

Query: 495 ALLSISFTIAVYVSTLVIIIVHKTT---GGHGKPDWLTNDLNDGRVDYFYFIVAGIAFFN 549
           A  S S  ++ YV+T +++IVH+TT   G HGKPDWLTNDLN GR+DYFY++VA  AFFN
Sbjct: 499 AFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLTNDLNKGRLDYFYYVVAATAFFN 558

BLAST of Sed0023549 vs. ExPASy TrEMBL
Match: A0A0A0KTW3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G604170 PE=3 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 1.1e-192
Identity = 341/563 (60.57%), Postives = 420/563 (74.60%), Query Frame = 0

Query: 5   NENPTSNAFVSSSQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTAT 64
           N NP  +   + S+R+PGGWK+MPYILGNETFERLA+ GLLANFMVYL+ +YHM+QV+AT
Sbjct: 23  NNNPKLSIISNKSKREPGGWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSAT 82

Query: 65  SVINIWSIANNCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCN 124
           S++ +W+   +  PL+GAF+SDAY+GR++TIAIASI S LGM  MT TAW+P LHPP C 
Sbjct: 83  SLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCG 142

Query: 125 APLEATDQCKGPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSF 184
             +    +C  PT+SQLGFLIMSLCL+SIGSGGIRPCS+PFGVDQFD TTEKGRKG+ SF
Sbjct: 143 GSV----KCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASF 202

Query: 185 YNLYYASFSIILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVG 244
           YN YYA+F ++LV T T+VVYIQDSVSWVLGY IPTVLMFC+I+LFF+GT VYV++KP G
Sbjct: 203 YNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEG 262

Query: 245 SVFTGLAQVLVATYKKRNLKLSSNGAIDQPTFYDPPLDSGTAVVSKLPLTIQ-------- 304
           S+FTGLAQV VA YKKR+LKL      D+  FYDPPLD  + V+SKLPLT Q        
Sbjct: 263 SIFTGLAQVAVAAYKKRHLKLPD----DRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAA 322

Query: 305 -------------------------------YRVLPIWGSTILCLLTIVQQGTFSVSQAL 364
                                          +RV PIW + IL L  I+QQ TFS+SQAL
Sbjct: 323 IKTENDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQAL 382

Query: 365 QMDRHMGPKFQVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGS 424
           +M+RHMG  FQ+P ASI VIS+LT++ +I  YD+  VP LRK T HPNGIT+LQR+ IG 
Sbjct: 383 KMNRHMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGI 442

Query: 425 VFAVMAMVIAGIIEKERRNMALRHNQKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFP 484
           VFAV++MV+AG+IE +RRN A  +   MSVFWL PQ  LMGLCEAF+IIGQ+EFFN EFP
Sbjct: 443 VFAVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFP 502

Query: 485 EHLRTMANALLSISFTIAVYVSTLVIIIVHKTT---GGHGKPDWLTNDLNDGRVDYFYFI 526
           EH+RTM NA  S S  ++ Y++T +++IVH+TT   G HGKPDWL +DLN GR+DYFY++
Sbjct: 503 EHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYV 562

BLAST of Sed0023549 vs. ExPASy TrEMBL
Match: A0A1S3BEQ4 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 2.13-like OS=Cucumis melo OX=3656 GN=LOC103489074 PE=3 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 3.1e-192
Identity = 343/563 (60.92%), Postives = 420/563 (74.60%), Query Frame = 0

Query: 5   NENPTSNAFVSSSQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTAT 64
           N N    +  + S+R+PGGWK+MPYILGNETFERLAS GLLANFMVYL++VYHM+QV+AT
Sbjct: 23  NNNNPKLSISNKSKREPGGWKSMPYILGNETFERLASMGLLANFMVYLKTVYHMDQVSAT 82

Query: 65  SVINIWSIANNCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCN 124
           S++ +W+   +  PL+GAF+SDAY+GR++TIAIASI S LGM  MT TAW+P LHPP C 
Sbjct: 83  SLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCG 142

Query: 125 APLEATDQCKGPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSF 184
             +    +C  PT+SQLGFLIMSLCL+SIGSGGIRPCS+PFGVDQFD TTEKGRKG+ SF
Sbjct: 143 GSV----KCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASF 202

Query: 185 YNLYYASFSIILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVG 244
           YN YYA+F ++LVFT T+VVYIQDSVSWVLGY IPT LMFC+I+LFF+GT VYV++KP G
Sbjct: 203 YNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCSIILFFMGTHVYVFIKPEG 262

Query: 245 SVFTGLAQVLVATYKKRNLKLSSNGAIDQPTFYDPPLDSGTAVVSKLPLTIQ-------- 304
           S+FTGLAQV VA YKKR+LKL      D+  FYDPPLD  + V+SKLPLT Q        
Sbjct: 263 SIFTGLAQVAVAAYKKRHLKLPD----DRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAA 322

Query: 305 -------------------------------YRVLPIWGSTILCLLTIVQQGTFSVSQAL 364
                                          +RV PIW + IL L  I+QQ TFS+SQAL
Sbjct: 323 IVTENEIKPDGTRLNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQAL 382

Query: 365 QMDRHMGPKFQVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGS 424
           QM+RHMG  FQ+P ASI VIS+LT++ +I LYDR  VP LRK T HPNGIT+LQR+ IG 
Sbjct: 383 QMNRHMGSNFQIPPASIIVISFLTITFFIPLYDRFLVPTLRKFTGHPNGITELQRMAIGI 442

Query: 425 VFAVMAMVIAGIIEKERRNMALRHNQKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFP 484
           +FAV++MV+AG++E +RRN A      MSVFWL PQ  LMGLCEAF+IIGQ+EFFN EFP
Sbjct: 443 IFAVLSMVVAGLVEMQRRNQANNDGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFP 502

Query: 485 EHLRTMANALLSISFTIAVYVSTLVIIIVHKTT---GGHGKPDWLTNDLNDGRVDYFYFI 526
           EH+RTM NA  S S  ++ Y++T +++IVH+TT   G HGKPDWL +DLN GR+DYFY+I
Sbjct: 503 EHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYI 562

BLAST of Sed0023549 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 570.9 bits (1470), Expect = 1.1e-162
Identity = 298/596 (50.00%), Postives = 406/596 (68.12%), Query Frame = 0

Query: 7   NPTSNAFVSSSQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTATSV 66
           +P S       ++KPGGW+ + +ILGNET ERL S GLLANFMVYL  V+H+ QV A +V
Sbjct: 38  SPGSILDAEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANV 97

Query: 67  INIWSIANNCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCNA- 126
           INIWS   N TPLVGA+ISD Y+GRF TIA AS  +LLG++ +T TA  P LHP  CN+ 
Sbjct: 98  INIWSGFTNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQ 157

Query: 127 -PLEATDQCKGPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSF 186
            PL     C GP   Q+G L++ LC LS+GSGGIRPCS+PFGVDQFD  TE+G KG+ SF
Sbjct: 158 DPL----SCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASF 217

Query: 187 YNLYYASFSIILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVG 246
           +N YY +F+++L+ T TVVVYIQD VSW++G++IPT LM  A+V+FF G + YVY+KP G
Sbjct: 218 FNWYYMTFTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEG 277

Query: 247 SVFTGLAQVLVATYKKRNLKL--SSNGAIDQPTFYDPPLDSGTAVVSKLPLTIQY----- 306
           S+F+G+AQV+VA  KKR LKL    +G +   T+YDP + S  +V+SKL  + Q+     
Sbjct: 278 SIFSGIAQVIVAARKKRKLKLPAEDDGTV---TYYDPAIKS--SVLSKLHRSNQFRCLDK 337

Query: 307 ----------------------------------RVLPIWGSTILCLLTIVQQGTFSVSQ 366
                                             R++PIW + I+ L  +  QGTF+VSQ
Sbjct: 338 AAVVIEGDLTPEGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQ 397

Query: 367 ALQMDRHMGPKFQVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGI 426
           AL+MDR++GPKF++PA S++VIS LT+ I++  YDRVFVP +R+IT H +GIT LQRIG 
Sbjct: 398 ALKMDRNLGPKFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGT 457

Query: 427 GSVFAVMAMVIAGIIEKERRNMALRHNQ-----KMSVFWLVPQHSLMGLCEAFSIIGQLE 486
           G VFA+ +M++AGI+E+ RR  ++          MSVFWL PQ  LMGLCEAF+IIGQ+E
Sbjct: 458 GIVFAIFSMIVAGIVERMRRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIE 517

Query: 487 FFNNEFPEHLRTMANALLSISFTIAVYVSTLVIIIVHKTTGGHGKPDWLTNDLNDGRVDY 546
           FFN++FPEH+R++AN+L S+SF  + Y+S+ ++ +VHK +GGH +PDWL  +LN G++DY
Sbjct: 518 FFNSQFPEHMRSIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDY 577

BLAST of Sed0023549 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 515.8 bits (1327), Expect = 4.3e-146
Identity = 270/548 (49.27%), Postives = 369/548 (67.34%), Query Frame = 0

Query: 18  QRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTATSVINIWSIANNCT 77
           ++K GGW+ + +ILGNET E+L S G+ ANFM+YLR+V+HM  V A +V  +W    N  
Sbjct: 12  EKKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFA 71

Query: 78  PLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCNAPLEATDQCKGPT 137
           PL+GA ISDAY+GRF TIA AS+ S+LG++ +T TA +P LHPP CN P    D+C  P 
Sbjct: 72  PLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNP--HPDECDDPN 131

Query: 138 SSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSFYNLYYASFSIILV 197
             QLG L + L  LSIGSGGIRPCS+PFGVDQFD  TE+G KG+ SF+N YY + +++L+
Sbjct: 132 KLQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLI 191

Query: 198 FTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVGSVFTGLAQVLVAT 257
           F+ TVVVY+Q +VSWV+G++IPT LM CA+VLFF+G R YVY+KP GSVF+G+A+V+VA 
Sbjct: 192 FSHTVVVYLQ-TVSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAA 251

Query: 258 YKKRNLKLSSNGAIDQPT--FYDPPLDSGTAVVSKLPLTIQY------------------ 317
            KKR+LK+S    +D  T  +Y+PP+  G  V+SKLPLT Q+                  
Sbjct: 252 RKKRDLKIS---LVDDGTEEYYEPPVKPG--VLSKLPLTDQFKFLDKAAVILDGDLTSEG 311

Query: 318 ---------------------RVLPIWGSTILCLLTIVQQGTFSVSQALQMDRHMGPKFQ 377
                                RV+P+W + I+ ++ +  Q TF V QA +MDRHMGP F+
Sbjct: 312 VPANKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFE 371

Query: 378 VPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGSVFAVMAMVIAG 437
           +PAASI VISY+T+ IW+ +Y+ + VP L ++ K    +T LQR+GIG VFA+++M  AG
Sbjct: 372 IPAASITVISYITIGIWVPIYEHLLVPFLWRMRKF--RVTLLQRMGIGIVFAILSMFTAG 431

Query: 438 IIEKERRNMALRHNQKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFPEHLRTMANALL 497
            +E  RR  A    Q MSVFWL     LMGLCE+F+ IG +EFFN++FPEH+R++AN+L 
Sbjct: 432 FVEGVRRTRATEMTQ-MSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLF 491

Query: 498 SISFTIAVYVSTLVIIIVHKTTGGHGKPDWLTNDLNDGRVDYFYFIVAGIAFFNLFLFVY 525
            +SF  A Y+S+L++  VHK +G    PDWL  DL+ G++DYFY+++A +   NL  F Y
Sbjct: 492 PLSFAAANYLSSLLVTTVHKVSGTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVNLVYFWY 548

BLAST of Sed0023549 vs. TAIR 10
Match: AT1G69860.1 (Major facilitator superfamily protein )

HSP 1 Score: 469.2 bits (1206), Expect = 4.7e-132
Identity = 250/556 (44.96%), Postives = 365/556 (65.65%), Query Frame = 0

Query: 5   NENPTSNAFVSSSQRKPGGWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTAT 64
           NE  TS++ +++ QRKP GWK MPYI+GNET ERLA+ GL+ANFMVY+   YHM+QV A 
Sbjct: 3   NEKGTSSSDLTTRQRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQAV 62

Query: 65  SVINIWSIANNCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCN 124
           ++IN WS   N  P++GAFISD+Y G+F TI   SI  LLGM+ +TFT+ +P+L PP C 
Sbjct: 63  TLINTWSALTNFAPIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPCT 122

Query: 125 APLEATDQCKGPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSF 184
           A  + T QC   + SQL  L+  L LLS+G+GGIR CSVPF +DQFD +TE+GR+G  SF
Sbjct: 123 AD-QITGQCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSF 182

Query: 185 YNLYYASFSIILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVG 244
           ++ YY + +I+ + + T+V+Y+Q+++SW +G+AIPTVL F A++L F+GTR YV++KP G
Sbjct: 183 FSWYYTTHTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEG 242

Query: 245 SVFTGLAQVLVATYKKRNLKLSSNGAIDQPTFYDPPLDSGTAVVSKLPLTIQYR------ 304
           SVF+G+ +VLVA YKKR  + +S   ID    Y  PL       +KL LT Q+R      
Sbjct: 243 SVFSGVFKVLVAAYKKRKARFTS--GID----YHQPLLETDLQSNKLVLTDQFRFLNKAV 302

Query: 305 -----------------------------VLPIWGSTILCLLTIVQQGTFSVSQALQMD- 364
                                        ++PI+ S+I+  L + QQ TF+VSQAL+MD 
Sbjct: 303 IVMNNDEAGNEEWRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDL 362

Query: 365 RHMGPKFQVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGSVFA 424
           +  G  + +P ASI VIS L + IW+  Y+ V V  +  ITK   GI+ LQ++GIG++F+
Sbjct: 363 QFPGTSYLIPPASITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFS 422

Query: 425 VMAMVIAGIIEKERRNMALRHNQKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFPEHL 484
           +  M+I+GI+E++RR+++L +  KMSVFWL PQ  LMG  + F+I+G  EFFN + P ++
Sbjct: 423 ISTMLISGIVERKRRDLSL-NGVKMSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINM 482

Query: 485 RTMANALLSISFTIAVYVSTLVIIIVHKTTGGHGKPDWLTNDLNDGRVDYFYFIVAGIAF 525
           R++ N+LL +  ++A Y+S+ ++ IVH  T   G   WLT+D++  ++D FY+ +A ++ 
Sbjct: 483 RSIGNSLLYLGLSLASYLSSAMVSIVHSVT-ARGGQSWLTDDIDKSKLDCFYYFIAALST 542

BLAST of Sed0023549 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 401.7 bits (1031), Expect = 9.1e-112
Identity = 218/560 (38.93%), Postives = 322/560 (57.50%), Query Frame = 0

Query: 23  GWKTMPYILGNETFERLASTGLLANFMVYLRSVYHMNQVTATSVINIWSIANNCTPLVGA 82
           GWK MP+I+GNETFE+L   G L+N +VYL +V+++  +TA ++IN +S   N    V A
Sbjct: 46  GWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTINFGTFVAA 105

Query: 83  FISDAYLGRFFTIAIASIVSLLGMVGMTFTAWIPHLHPPKCNAPLEATDQCKGPTSSQLG 142
           F+ D Y GR+ T+++A I   LG   +  TA +P LHP  C    ++   C GP+  Q+ 
Sbjct: 106 FLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSI--CNGPSGGQIA 165

Query: 143 FLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTTEKGRKGLGSFYNLYYASFSIILVFTGTV 202
           FL+M L  L +G+GGIRPC++ FG DQF+  +E G++G+ SF+N Y+ +F+   + + T+
Sbjct: 166 FLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQILSLTL 225

Query: 203 VVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGTRVYVYLKPVGSVFTGLAQVLVATYKKRN 262
           VVY+Q +VSW +G  IP VLMF A ++FF G ++YV +K  GS   G+AQV+    KKR 
Sbjct: 226 VVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIKKRG 285

Query: 263 LKLSSNGAIDQPTFYDPPLDSGTAVVSKLPLTIQY------------------------- 322
           LK +    ++   +Y P   +     SKL  T Q+                         
Sbjct: 286 LKPAKQPWLNLYNYYPPKYAN-----SKLKYTDQFRFLDKAAILTPEDKLQPDGKPADPW 345

Query: 323 ---------------RVLPIWGSTILCLLTIVQQGTFSVSQALQMDRHMGP-KFQVPAAS 382
                          RVLPIW ++ +  LTI QQ T+ V QALQ DR +G   F +PAA+
Sbjct: 346 KLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAAT 405

Query: 383 IAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQLQRIGIGSVFAVMAMVIAGIIEKE 442
             V     ++++I++YDRV VP +R+IT    GIT LQRIG G  FA  ++V+AG +E+ 
Sbjct: 406 YVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEER 465

Query: 443 RRNMALR-----------HNQKMSVFWLVPQHSLMGLCEAFSIIGQLEFFNNEFPEHLRT 502
           RR  AL                MS  WL+PQ SL G+ EAF+ IGQ+EF+  +FPE++R+
Sbjct: 466 RRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMRS 525

Query: 503 MANALLSISFTIAVYVSTLVIIIVHKTTGGHGKPDWLTNDLNDGRVDYFYFIVAGIAFFN 531
            A ++  +   ++ Y+ + +I  VH+TT      +WL  DLN GR+D FYF++AGI   N
Sbjct: 526 FAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWLAEDLNKGRLDLFYFMIAGILAVN 585

BLAST of Sed0023549 vs. TAIR 10
Match: AT3G47960.1 (Major facilitator superfamily protein )

HSP 1 Score: 395.2 bits (1014), Expect = 8.6e-110
Identity = 231/597 (38.69%), Postives = 340/597 (56.95%), Query Frame = 0

Query: 1   METQNENPTSNAFVSSS----QRK--PGGWKTMPYILGNETFERLASTGLLANFMVYLRS 60
           +E +    T+N  V  S    QRK    GWK MP+I+GNETFE+L   G L+N +VYL S
Sbjct: 36  LEVEPSTTTTNTDVVDSFEEEQRKIVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTS 95

Query: 61  VYHMNQVTATSVINIWSIANNCTPLVGAFISDAYLGRFFTIAIASIVSLLGMVGMTFTAW 120
           V+++   TA ++IN +S   N    + AF+ D Y GR+ T+++A I   LG   +  TA 
Sbjct: 96  VFNLKSYTAATIINAFSGTINFGTFIAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAA 155

Query: 121 IPHLHPPKCNAPLEATDQCKGPTSSQLGFLIMSLCLLSIGSGGIRPCSVPFGVDQFDTTT 180
           IP LHP  C   +     C+GP+  Q+ FL+M L  L +G+GGIRPC++ FG DQF+  +
Sbjct: 156 IPSLHPVACGNKI----SCEGPSVGQILFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKS 215

Query: 181 EKGRKGLGSFYNLYYASFSIILVFTGTVVVYIQDSVSWVLGYAIPTVLMFCAIVLFFLGT 240
           E G+KG+ SF+N Y+ +F+   + + T VVYIQ +VSW +G  IP  LMF A V+FF G 
Sbjct: 216 ESGKKGINSFFNWYFFTFTFAQIISLTAVVYIQSNVSWTIGLIIPVALMFLACVIFFAGD 275

Query: 241 RVYVYLKPVGSVFTGLAQVLVATYKKRNLK------------LSSNGA------IDQPTF 300
           R+YV +K  GS   G+A+V+ A  KKR LK            + SN A       DQ  F
Sbjct: 276 RLYVKVKASGSPLAGIARVIAAAIKKRGLKPVKQPWVNLYNHIPSNYANTTLKYTDQFRF 335

Query: 301 YDPPL---------DSGTAVVSKLPLTIQ--------YRVLPIWGSTILCLLTIVQQGTF 360
            D              GTA       T+Q         RV+PIW ++ +  L I  Q T+
Sbjct: 336 LDKAAIMTPEEKLNSDGTASDPWKLCTLQQVEEVKCIVRVIPIWFASTIYYLAITIQMTY 395

Query: 361 SVSQALQMDRHMGP-KFQVPAASIAVISYLTLSIWILLYDRVFVPRLRKITKHPNGITQL 420
            V QALQ DR +G   F++PAA+  V     ++++I+ YDRV VP LR++T    GI+ L
Sbjct: 396 PVFQALQSDRRLGSGGFRIPAATYVVFLMTGMTVFIIFYDRVLVPSLRRVTGLETGISLL 455

Query: 421 QRIGIGSVFAVMAMVIAGIIEKERRNMALR-----------HNQKMSVFWLVPQHSLMGL 480
           QRIG G  FA+M+++++G IE+ RRN AL                MS  WL+PQ +L G+
Sbjct: 456 QRIGAGFTFAIMSLLVSGFIEERRRNFALTKPTLGMAPRTGEISSMSALWLIPQLTLAGI 515

Query: 481 CEAFSIIGQLEFFNNEFPEHLRTMANALLSISFTIAVYVSTLVIIIVHKTTGGHGKPDWL 540
            EAF+ IGQ+EF+  +FPE++++ A ++  +   ++ Y+++ +I  VH+TT      +WL
Sbjct: 516 AEAFAAIGQMEFYYKQFPENMKSFAGSIFYVGAGVSSYLASFLISTVHRTTAHSPSGNWL 575

Query: 541 TNDLNDGRVDYFYFIVAGIAFFNLFLFVYCARSYRYKGTGENLGVKPMDEVELSSEK 545
             DLN  ++DYFYF++ G+   N+  F+  AR YRYKG  +    + + E+E + E+
Sbjct: 576 AEDLNKAKLDYFYFMLTGLMVVNMAYFLLMARWYRYKGGND----EDITEIETNEEE 624