Sed0023117 (gene) Chayote v1

BLAST of Sed0023117 vs. NCBI nr
Match: XP_023551263.1 (GDSL esterase/lipase At3g27950-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 551.6 bits (1420), Expect = 4.8e-153
Identity = 279/381 (73.23%), Postives = 303/381 (79.53%), Query Frame = 0

Query: 1   MDPVKRRRLLVVLILGQTVAFICAAAGG--CGFPAVYNFGDSNSDTGGVSAAMKGIQPPN 60
           M  +K RRLLVVLILG  +    A  G   CGFPAVYNFGDSNSDTGG+SAA   IQPPN
Sbjct: 1   MGLLKLRRLLVVLILGLLIGVTTAGGGSGKCGFPAVYNFGDSNSDTGGISAAFNAIQPPN 60

Query: 61  GETFFGHPAGRLCDGRLIVDFIAEKLKLPYLSTYLDSLSTNFRHGANFATAGSSIRPGGY 120
           GETFF  P+GR CDGRLIVDFIAEKLKLPYLS YLDSL TNFRHGANFAT GSSI PGGY
Sbjct: 61  GETFFRRPSGRACDGRLIVDFIAEKLKLPYLSAYLDSLGTNFRHGANFATGGSSICPGGY 120

Query: 121 NPFHLGIQVSQFIQFKSRTTDLYNRLRSNNRTPLPIKRSIARPWEFPKALYMFDIGQNDL 180
           +PFHLGIQVSQFIQFKSRTTDL+NRL SNN+T +PIKR IARP EF KALY FDIGQNDL
Sbjct: 121 SPFHLGIQVSQFIQFKSRTTDLFNRLHSNNKTSIPIKR-IARPQEFSKALYTFDIGQNDL 180

Query: 181 GYGYQHTSEEQVRASIPDILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYNSSPH 240
            YGYQH+SEEQVRASIPDIL+TFSEAVQQLYKEGARYFWVHNTGPIGCL Y ILYN SP 
Sbjct: 181 AYGYQHSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPD 240

Query: 241 DRDSNGCVKSQNKVAQEFNWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGFV 300
           +RDSNGCV+SQN+V++EFN QLK+ LLKLGKKLP+ RI  +DMYSAKYLLI+ AK+ GFV
Sbjct: 241 NRDSNGCVESQNEVSREFNRQLKSRLLKLGKKLPVARIIHIDMYSAKYLLITEAKTQGFV 300

Query: 301 NN---------------------------------PSRHITWDGIHYSEAANLWIANHIM 347
            N                                 PSRHI+WDGIHYSEAANLWIANHI+
Sbjct: 301 KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSEAANLWIANHIL 360

BLAST of Sed0023117 vs. NCBI nr
Match: XP_022938610.1 (GDSL esterase/lipase At3g27950-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 550.4 bits (1417), Expect = 1.1e-152
Identity = 279/381 (73.23%), Postives = 302/381 (79.27%), Query Frame = 0

Query: 1   MDPVKRRRLLVVLILGQTVAFICAAAGG--CGFPAVYNFGDSNSDTGGVSAAMKGIQPPN 60
           M  +K RRLLVVLILG  +    A  G   CGFPAVYNFGDSNSDTGG+SAA   IQPPN
Sbjct: 1   MGLLKLRRLLVVLILGLLIGVTTAGGGSGKCGFPAVYNFGDSNSDTGGISAAFNAIQPPN 60

Query: 61  GETFFGHPAGRLCDGRLIVDFIAEKLKLPYLSTYLDSLSTNFRHGANFATAGSSIRPGGY 120
           GETFF  P+GR CDGRLIVDFIAEKLKLPYLS YLDSL TNFRHGANFAT GSSI PGGY
Sbjct: 61  GETFFRRPSGRACDGRLIVDFIAEKLKLPYLSAYLDSLGTNFRHGANFATGGSSICPGGY 120

Query: 121 NPFHLGIQVSQFIQFKSRTTDLYNRLRSNNRTPLPIKRSIARPWEFPKALYMFDIGQNDL 180
           +PFHLGIQVSQFIQFKSR TDL+NRL SNN+T +PIKR IARP EF KALY FDIGQNDL
Sbjct: 121 SPFHLGIQVSQFIQFKSRITDLFNRLHSNNKTSIPIKR-IARPQEFSKALYTFDIGQNDL 180

Query: 181 GYGYQHTSEEQVRASIPDILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYNSSPH 240
            YGYQH+SEEQVRASIPDIL+TFSEAVQQLYKEGARYFWVHNTGPIGCL Y ILYN SP 
Sbjct: 181 AYGYQHSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPD 240

Query: 241 DRDSNGCVKSQNKVAQEFNWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGFV 300
           +RDSNGCV+SQN+V++EFN QLK+ LLKLGKKLPL RI  +DMYSAKYLLI+ AK+ GFV
Sbjct: 241 NRDSNGCVESQNEVSREFNRQLKSRLLKLGKKLPLARIIHIDMYSAKYLLITEAKTQGFV 300

Query: 301 NN---------------------------------PSRHITWDGIHYSEAANLWIANHIM 347
            N                                 PSRHI+WDGIHYSEAANLWIANHI+
Sbjct: 301 KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSEAANLWIANHIL 360

BLAST of Sed0023117 vs. NCBI nr
Match: XP_004142359.1 (GDSL esterase/lipase At3g27950 [Cucumis sativus] >KGN52374.1 hypothetical protein Csa_009178 [Cucumis sativus])

HSP 1 Score: 547.0 bits (1408), Expect = 1.2e-151
Identity = 275/382 (71.99%), Postives = 299/382 (78.27%), Query Frame = 0

Query: 1   MDPVKRRRLLVVLIL---GQTVAFICAAAGGCGFPAVYNFGDSNSDTGGVSAAMKGIQPP 60
           M+ +K R L  +LIL   G+TV     AA  CGFPA+YNFGDSNSDTGG+SAA+  IQPP
Sbjct: 1   MNSLKLRHLWALLILGLVGRTVTLFGDAARTCGFPAIYNFGDSNSDTGGISAALNAIQPP 60

Query: 61  NGETFFGHPAGRLCDGRLIVDFIAEKLKLPYLSTYLDSLSTNFRHGANFATAGSSIRPGG 120
           NGETFFGHP+GR CDGRLI+DFIAEKLKLPYLS YLDSL T+FRHGANFAT GSSIRPGG
Sbjct: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPGG 120

Query: 121 YNPFHLGIQVSQFIQFKSRTTDLYNRLRSNNRTPLPIKRSIARPWEFPKALYMFDIGQND 180
           Y+PFHLGIQVSQFIQFKSRTTDLYNRLRS  RT +PI+  IARP EF KALY FDIGQND
Sbjct: 121 YSPFHLGIQVSQFIQFKSRTTDLYNRLRSRIRTSIPIEH-IARPQEFSKALYTFDIGQND 180

Query: 181 LGYGYQHTSEEQVRASIPDILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYNSSP 240
           L YGYQH+SEEQVRASIPDIL  F EAVQQLYKEGARYFWVHNTGPIGCL Y ILYN SP
Sbjct: 181 LAYGYQHSSEEQVRASIPDILDAFCEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP 240

Query: 241 HDRDSNGCVKSQNKVAQEFNWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGF 300
            +RDSNGCVKSQN V++EFN QLK+ LLKLGKKLP  RI  VD+YS KYLLI+ AK+ GF
Sbjct: 241 ENRDSNGCVKSQNTVSREFNRQLKSQLLKLGKKLPFARIIHVDVYSVKYLLITKAKTQGF 300

Query: 301 VNN---------------------------------PSRHITWDGIHYSEAANLWIANHI 347
           V N                                 PSRHI+WDGIHYSEAANLWIANHI
Sbjct: 301 VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCEDPSRHISWDGIHYSEAANLWIANHI 360

BLAST of Sed0023117 vs. NCBI nr
Match: XP_022134509.1 (GDSL esterase/lipase At5g14450 [Momordica charantia])

HSP 1 Score: 544.7 bits (1402), Expect = 5.9e-151
Identity = 275/386 (71.24%), Postives = 302/386 (78.24%), Query Frame = 0

Query: 1   MDPVKRRRLLVVLILGQTVAFICAAAGG--------CGFPAVYNFGDSNSDTGGVSAAMK 60
           M P+K R +LV+LILG     + ++ GG        CGFPAVYNFGDSNSDTGG+SAA  
Sbjct: 1   MCPLKLRPVLVLLILGLVGRPVASSGGGGGRGGSGSCGFPAVYNFGDSNSDTGGISAAFN 60

Query: 61  GIQPPNGETFFGHPAGRLCDGRLIVDFIAEKLKLPYLSTYLDSLSTNFRHGANFATAGSS 120
            IQ PNGETFFGHP+GR CDGRLI+DFIAEKLKLPYLS YLDS+ T+FRHGANFAT GSS
Sbjct: 61  VIQSPNGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSVGTSFRHGANFATGGSS 120

Query: 121 IRPGGYNPFHLGIQVSQFIQFKSRTTDLYNRLRSNNRTPLPIKRSIARPWEFPKALYMFD 180
           IRPGGY+PFHLGIQVSQFIQFKSRTT+LY +LRSNNRT LPIK SIARP EF KALY FD
Sbjct: 121 IRPGGYSPFHLGIQVSQFIQFKSRTTNLYYQLRSNNRTSLPIKSSIARPGEFSKALYTFD 180

Query: 181 IGQNDLGYGYQHTSEEQVRASIPDILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPIL 240
           I QNDL YGYQH+SEEQVRASIPDIL+TFSEAVQQLYKEGARYFWVHNTGP+GCL + IL
Sbjct: 181 IAQNDLSYGYQHSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPLGCLPFNIL 240

Query: 241 YNSSPHDRDSNGCVKSQNKVAQEFNWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNA 300
           YN +P++ DS GCVKSQN+VA+E NWQLKNLLL L K LPL RIT VDM+SAKY LISNA
Sbjct: 241 YNKNPNNLDSIGCVKSQNEVARELNWQLKNLLLNLKKMLPLARITHVDMHSAKYQLISNA 300

Query: 301 KSLGFVNN---------------------------------PSRHITWDGIHYSEAANLW 346
           K+ GFVNN                                 PSRHI+WDGIHYSEAANLW
Sbjct: 301 KTQGFVNNPVKFCCGSYYGFHINCGKKEVVNGTVYGSPCKDPSRHISWDGIHYSEAANLW 360

BLAST of Sed0023117 vs. NCBI nr
Match: XP_008458756.1 (PREDICTED: GDSL esterase/lipase At3g27950-like isoform X1 [Cucumis melo] >XP_008458757.1 PREDICTED: GDSL esterase/lipase At3g27950-like isoform X1 [Cucumis melo] >XP_016902334.1 PREDICTED: GDSL esterase/lipase At3g27950-like isoform X1 [Cucumis melo])

HSP 1 Score: 544.3 bits (1401), Expect = 7.7e-151
Identity = 274/382 (71.73%), Postives = 301/382 (78.80%), Query Frame = 0

Query: 1   MDPVKRRRLLVVLIL---GQTVAFICAAAGGCGFPAVYNFGDSNSDTGGVSAAMKGIQPP 60
           M+ +K RRL  +LIL   G+TV    AAAG CGFPA+YNFGDSNSDTGG+SAA+  IQPP
Sbjct: 1   MNSLKLRRLWALLILGLVGRTVTLFGAAAGTCGFPAIYNFGDSNSDTGGISAALNAIQPP 60

Query: 61  NGETFFGHPAGRLCDGRLIVDFIAEKLKLPYLSTYLDSLSTNFRHGANFATAGSSIRPGG 120
           NGETFFGHP+GR CDGRLI+DFIAEKLKLPYLS YLDS+ T+FRHGANFAT GSSIRPGG
Sbjct: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSIGTSFRHGANFATGGSSIRPGG 120

Query: 121 YNPFHLGIQVSQFIQFKSRTTDLYNRLRSNNRTPLPIKRSIARPWEFPKALYMFDIGQND 180
           Y+PFHLGIQVSQFIQFKSRTTDL+NRLRS+NRT +PIK  IARP EF KALY FDIGQND
Sbjct: 121 YSPFHLGIQVSQFIQFKSRTTDLFNRLRSHNRTAIPIKH-IARPQEFSKALYTFDIGQND 180

Query: 181 LGYGYQHTSEEQVRASIPDILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYNSSP 240
           L YGYQH+SEEQVRASIPDIL TF EAVQQLYKEGARYFWVHNTGPIGCL Y ILYN SP
Sbjct: 181 LAYGYQHSSEEQVRASIPDILDTFCEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP 240

Query: 241 HDRDSNGCVKSQNKVAQEFNWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGF 300
            +RDS+GCVKSQN V++EFN QLK+ LLKL KKLP  RI  VDMYS KYLLI+ AKS GF
Sbjct: 241 ENRDSSGCVKSQNTVSREFNRQLKSQLLKLRKKLPFARIVHVDMYSVKYLLITKAKSQGF 300

Query: 301 VNN---------------------------------PSRHITWDGIHYSEAANLWIANHI 347
           V N                                 PSR I+WDGIHYSEAAN WIANHI
Sbjct: 301 VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCEDPSRQISWDGIHYSEAANSWIANHI 360

BLAST of Sed0023117 vs. ExPASy Swiss-Prot
Match: Q9LII9 (GDSL esterase/lipase At3g27950 OS=Arabidopsis thaliana OX=3702 GN=At3g27950 PE=2 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 2.9e-92
Identity = 185/368 (50.27%), Postives = 233/368 (63.32%), Query Frame = 0

Query: 10  LVVLILGQTVAFICAAAGGCGFPAVYNFGDSNSDTGGVSAAMKGIQPPNGETFFGHPAGR 69
           ++VL+LG T   + A +  C FPAV+NFGDSNSDTG +SAA+  + PPNG  FFG  AGR
Sbjct: 10  IIVLLLGFTEK-LSALSSSCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFFGRSAGR 69

Query: 70  LCDGRLIVDFIAEKLKLPYLSTYLDSLSTNFRHGANFATAGSSIRP--GGYNPFHLGIQV 129
             DGRLI+DFI E L LPYL+ YLDS+  N+RHGANFAT GS IRP    ++PFHLG QV
Sbjct: 70  HSDGRLIIDFITENLTLPYLTPYLDSVGANYRHGANFATGGSCIRPTLACFSPFHLGTQV 129

Query: 130 SQFIQFKSRTTDLYNRLRSN-NRTPLPIKRSIARPWEFPKALYMFDIGQNDLGYGYQHTS 189
           SQFI FK+RT  LYN+     NR        ++    F KALY  DIGQNDL  G+Q+ +
Sbjct: 130 SQFIHFKTRTLSLYNQTNGKFNR--------LSHTNYFSKALYTLDIGQNDLAIGFQNMT 189

Query: 190 EEQVRASIPDILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYNSSPHDRDSNGCV 249
           EEQ++A+IP I+  F+ A++ LYKEGAR+F +HNTGP GCL Y +L       RD  GC+
Sbjct: 190 EEQLKATIPLIIENFTIALKLLYKEGARFFSIHNTGPTGCLPY-LLKAFPAIPRDPYGCL 249

Query: 250 KSQNKVAQEFNWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGFVN------- 309
           K  N VA EFN QLKN + +L K+LP    T VD+YSAKY LI+ AK+LGF++       
Sbjct: 250 KPLNNVAIEFNKQLKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFIDPFDYCCV 309

Query: 310 --------------------------NPSRHITWDGIHYSEAANLWIANHIMNGSFSDPP 342
                                     N    I+WDGIHY+E AN+ +AN I++GS SDPP
Sbjct: 310 GAIGRGMGCGKTIFLNGTELYSSSCQNRKNFISWDGIHYTETANMLVANRILDGSISDPP 367

BLAST of Sed0023117 vs. ExPASy Swiss-Prot
Match: Q9LY84 (GDSL esterase/lipase At5g14450 OS=Arabidopsis thaliana OX=3702 GN=At5g14450 PE=2 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 6.3e-87
Identity = 168/356 (47.19%), Postives = 226/356 (63.48%), Query Frame = 0

Query: 29  CGFPAVYNFGDSNSDTGGVSAAMKGIQPPNGETFFGHPAGRLCDGRLIVDFIAEKLKLPY 88
           C FPA+YNFGDSNSDTGG+SAA + I+ P G+ FF  P GR  DGRL +DFIAE+L LPY
Sbjct: 37  CTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQGFFHRPTGRDSDGRLTIDFIAERLGLPY 96

Query: 89  LSTYLDSLSTNFRHGANFATAGSSIR-------PGGYNPFHLGIQVSQFIQFKSRTTDLY 148
           LS YL+SL +NFRHGANFAT GS+IR         G +PF L +Q++QF QFK+R+  L+
Sbjct: 97  LSAYLNSLGSNFRHGANFATGGSTIRRQNETIFQYGISPFSLDMQIAQFDQFKARSALLF 156

Query: 149 NRLRSN-NRTPLPIKRSIARPWEFPKALYMFDIGQNDLGYGYQHTSEEQVRASIPDILST 208
            +++S  +R  LP      R  EF KALY FDIGQNDL  G++  S +Q++A+IPDI++ 
Sbjct: 157 TQIKSRYDREKLP------RQEEFAKALYTFDIGQNDLSVGFRTMSVDQLKATIPDIVNH 216

Query: 209 FSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYNSSPHD--RDSNGCVKSQNKVAQEFNW 268
            + AV+ +Y++G R FWVHNTGP GCL   + Y  +P     D +GCVK+QN++A EFN 
Sbjct: 217 LASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPAPGYLDKSGCVKAQNEMAMEFNR 276

Query: 269 QLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGFVN------------------- 328
           +LK  ++ L K+L    IT VD+Y+AKY ++SN K LGF N                   
Sbjct: 277 KLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKLGFANPLKVCCGYHEKYDHIWCGN 336

Query: 329 ---------------NPSRHITWDGIHYSEAANLWIANHIMNGSFSDPPLSIDKAC 341
                          NP   ++WDG+HY+EAAN  +A+  +NG  +DPP+ I +AC
Sbjct: 337 KGKVNNTEIYGGSCPNPVMAVSWDGVHYTEAANKHVADRTLNGLLTDPPVPITRAC 386

BLAST of Sed0023117 vs. ExPASy Swiss-Prot
Match: B4FZ87 (GDSL esterase/lipase ACHE OS=Zea mays OX=4577 GN=ACHE PE=1 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 4.8e-79
Identity = 164/388 (42.27%), Postives = 218/388 (56.19%), Query Frame = 0

Query: 8   RLLVVLILGQTVAFI-----CAAAGGCGFPAVYNFGDSNSDTGGVSAAMKGIQPPNGETF 67
           R  V+L+L   +A         A G C FPAV+NFGDSNSDTGG+S+      PPNG TF
Sbjct: 12  RAAVLLLLSLALALALRPSDAGAGGDCHFPAVFNFGDSNSDTGGLSSLFGAAPPPNGRTF 71

Query: 68  FGHPAGRLCDGRLIVDFIAEKLKLPYLSTYLDSLSTNFRHGANFATAGSSIRP------- 127
           FG PAGR CDGRL++DFIAE L L +LS YL+S+ +NF  GANFATAGSSIR        
Sbjct: 72  FGMPAGRYCDGRLVIDFIAESLGLTHLSAYLNSIGSNFTQGANFATAGSSIRRQNTSLFL 131

Query: 128 GGYNPFHLGIQVSQFIQFKSRTTDLYNRLRSNNRTPLPIKRSIARPWEFPKALYMFDIGQ 187
            G++P  L +Q  +F QF +R+  +YN      R  LP      R   F +ALY FDIGQ
Sbjct: 132 SGFSPISLDVQFWEFEQFINRSQLVYNNKGGIYREILP------RAEYFSQALYTFDIGQ 191

Query: 188 NDLGYGY-QHTSEEQVRASIPDILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYN 247
           ND+   Y  + + E+V A IPD++   +  +Q +Y  G RYFW+HNTGP+GCL Y +L+ 
Sbjct: 192 NDITSSYFVNNTTEEVEAIIPDLMERLTSIIQSVYSRGGRYFWIHNTGPLGCLPYALLHR 251

Query: 248 SS-PHDRDSNGCVKSQNKVAQEFNWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAK 307
                  D  GC  + NKVAQ FN +LK  +  L K  P    T VD+Y+AKY LIS A 
Sbjct: 252 PDLAIPADGTGCSVTYNKVAQLFNLRLKETVASLRKTHPDAAFTYVDVYTAKYKLISQAN 311

Query: 308 SLGF---------------------------------------VNNPSRHITWDGIHYSE 343
            LGF                                         NPS+ ++WDG+H++E
Sbjct: 312 KLGFDDPLLTCCGYGGGRYNLDLSVGCGGKKQVNGTSVVVGKSCENPSKRVSWDGVHFTE 371

BLAST of Sed0023117 vs. ExPASy Swiss-Prot
Match: Q7Y1X1 (Esterase OS=Hevea brasiliensis OX=3981 PE=1 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 6.9e-78
Identity = 157/362 (43.37%), Postives = 210/362 (58.01%), Query Frame = 0

Query: 25  AAGGCGFPAVYNFGDSNSDTGGVSAAMKGIQPPNGETFFGHPAGRLCDGRLIVDFIAEKL 84
           A+  C FPA++NFGDSNSDTGG +AA   + PP GETFF    GR  DGRLI+DFIAE  
Sbjct: 26  ASETCDFPAIFNFGDSNSDTGGKAAAFYPLNPPYGETFFHRSTGRYSDGRLIIDFIAESF 85

Query: 85  KLPYLSTYLDSLSTNFRHGANFATAGSSIR--------PGGYNPFHLGIQVSQFIQFKSR 144
            LPYLS YL SL +NF+HGA+FATAGS+I+         GG++PF+L +Q SQF QF  R
Sbjct: 86  NLPYLSPYLSSLGSNFKHGADFATAGSTIKLPTTIIPAHGGFSPFYLDVQYSQFRQFIPR 145

Query: 145 TTDLYNRLRSNNRTPLPIKRSIARPWEFPKALYMFDIGQNDLGYGYQHTSEEQVRASIPD 204
           +  +         T       +   + F KALY FDIGQNDL  G+ + + E+V A++PD
Sbjct: 146 SQFI-------RETGGIFAELVPEEYYFEKALYTFDIGQNDLTEGFLNLTVEEVNATVPD 205

Query: 205 ILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYNSSPHDRDSNGCVKSQNKVAQEF 264
           ++++FS  V+++Y  GAR FW+HNTGPIGCL + + Y     ++DS GC K+ N+VAQ F
Sbjct: 206 LVNSFSANVKKIYDLGARTFWIHNTGPIGCLSFILTYFPWA-EKDSAGCAKAYNEVAQHF 265

Query: 265 NWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGF------------------- 324
           N +LK ++ +L K LPL     VD+YS KY L S  +  GF                   
Sbjct: 266 NHKLKEIVAQLRKDLPLATFVHVDIYSVKYSLFSEPEKHGFEFPLITCCGYGGKYNFSVT 325

Query: 325 -------------------VNNPSRHITWDGIHYSEAANLWIANHIMNGSFSDPPLSIDK 341
                                 PS  + WDG HY+EAAN +  + I  G+FSDPP+ ++ 
Sbjct: 326 APCGDTVTADDGTKIVVGSCACPSVRVNWDGAHYTEAANEYFFDQISTGAFSDPPVPLNM 379

BLAST of Sed0023117 vs. ExPASy Swiss-Prot
Match: Q9FXE5 (Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.0e-73
Identity = 154/360 (42.78%), Postives = 207/360 (57.50%), Query Frame = 0

Query: 29  CGFPAVYNFGDSNSDTGGVSAAMKGIQPPNGETFFGHPAGRLCDGRLIVDFIAEKLKLPY 88
           C FPA++NFGDSNSDTGG+SAA     PP+G +FFG PAGR CDGRL++DFIAE L LPY
Sbjct: 26  CHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSPAGRYCDGRLVIDFIAESLGLPY 85

Query: 89  LSTYLDSLSTNFRHGANFATAG-------SSIRPGGYNPFHLGIQVSQFIQFKSRTTDLY 148
           LS +LDS+ +NF HGANFATAG       S++R  G++PF L +Q  QF  F +R+  + 
Sbjct: 86  LSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFSLDVQFVQFYNFHNRSQTVR 145

Query: 149 NRLRSNNRTPLPIKRSIARPWEFPKALYMFDIGQNDLGYGY-QHTSEEQVRASIPDILST 208
           +R     +T LP   S      F KALY FDIGQNDL  GY  + + EQV   +P+I+S 
Sbjct: 146 SR-GGVYKTMLPESDS------FSKALYTFDIGQNDLTAGYFANKTVEQVETEVPEIISQ 205

Query: 209 FSEAVQQLYKEGARYFWVHNTGPIGCLHYPI-LYNSSPHDRDSNGCVKSQNKVAQEFNWQ 268
           F  A++ +Y +G RYFW+HNTGPIGCL Y I  + +   D DS+GCV   N +AQ+FN  
Sbjct: 206 FMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHGCVSPLNHLAQQFNHA 265

Query: 269 LKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGF---------------------- 328
           LK  +++L   L    IT VD+YS K+ L  +A+  GF                      
Sbjct: 266 LKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCGHGGKYNYNKGIGC 325

Query: 329 ----------------VNNPSRHITWDGIHYSEAANLWIANHIMNGSFSDPPLSIDKACQ 342
                            + P + + WDG+H+++AAN +I + I  G        + KAC+
Sbjct: 326 GMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKIAPG--------LSKACK 370

BLAST of Sed0023117 vs. ExPASy TrEMBL
Match: A0A6J1FK92 (GDSL esterase/lipase At3g27950-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444793 PE=3 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 5.2e-153
Identity = 279/381 (73.23%), Postives = 302/381 (79.27%), Query Frame = 0

Query: 1   MDPVKRRRLLVVLILGQTVAFICAAAGG--CGFPAVYNFGDSNSDTGGVSAAMKGIQPPN 60
           M  +K RRLLVVLILG  +    A  G   CGFPAVYNFGDSNSDTGG+SAA   IQPPN
Sbjct: 1   MGLLKLRRLLVVLILGLLIGVTTAGGGSGKCGFPAVYNFGDSNSDTGGISAAFNAIQPPN 60

Query: 61  GETFFGHPAGRLCDGRLIVDFIAEKLKLPYLSTYLDSLSTNFRHGANFATAGSSIRPGGY 120
           GETFF  P+GR CDGRLIVDFIAEKLKLPYLS YLDSL TNFRHGANFAT GSSI PGGY
Sbjct: 61  GETFFRRPSGRACDGRLIVDFIAEKLKLPYLSAYLDSLGTNFRHGANFATGGSSICPGGY 120

Query: 121 NPFHLGIQVSQFIQFKSRTTDLYNRLRSNNRTPLPIKRSIARPWEFPKALYMFDIGQNDL 180
           +PFHLGIQVSQFIQFKSR TDL+NRL SNN+T +PIKR IARP EF KALY FDIGQNDL
Sbjct: 121 SPFHLGIQVSQFIQFKSRITDLFNRLHSNNKTSIPIKR-IARPQEFSKALYTFDIGQNDL 180

Query: 181 GYGYQHTSEEQVRASIPDILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYNSSPH 240
            YGYQH+SEEQVRASIPDIL+TFSEAVQQLYKEGARYFWVHNTGPIGCL Y ILYN SP 
Sbjct: 181 AYGYQHSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPD 240

Query: 241 DRDSNGCVKSQNKVAQEFNWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGFV 300
           +RDSNGCV+SQN+V++EFN QLK+ LLKLGKKLPL RI  +DMYSAKYLLI+ AK+ GFV
Sbjct: 241 NRDSNGCVESQNEVSREFNRQLKSRLLKLGKKLPLARIIHIDMYSAKYLLITEAKTQGFV 300

Query: 301 NN---------------------------------PSRHITWDGIHYSEAANLWIANHIM 347
            N                                 PSRHI+WDGIHYSEAANLWIANHI+
Sbjct: 301 KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSEAANLWIANHIL 360

BLAST of Sed0023117 vs. ExPASy TrEMBL
Match: A0A0A0KTW4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G630770 PE=3 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 5.8e-152
Identity = 275/382 (71.99%), Postives = 299/382 (78.27%), Query Frame = 0

Query: 1   MDPVKRRRLLVVLIL---GQTVAFICAAAGGCGFPAVYNFGDSNSDTGGVSAAMKGIQPP 60
           M+ +K R L  +LIL   G+TV     AA  CGFPA+YNFGDSNSDTGG+SAA+  IQPP
Sbjct: 1   MNSLKLRHLWALLILGLVGRTVTLFGDAARTCGFPAIYNFGDSNSDTGGISAALNAIQPP 60

Query: 61  NGETFFGHPAGRLCDGRLIVDFIAEKLKLPYLSTYLDSLSTNFRHGANFATAGSSIRPGG 120
           NGETFFGHP+GR CDGRLI+DFIAEKLKLPYLS YLDSL T+FRHGANFAT GSSIRPGG
Sbjct: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPGG 120

Query: 121 YNPFHLGIQVSQFIQFKSRTTDLYNRLRSNNRTPLPIKRSIARPWEFPKALYMFDIGQND 180
           Y+PFHLGIQVSQFIQFKSRTTDLYNRLRS  RT +PI+  IARP EF KALY FDIGQND
Sbjct: 121 YSPFHLGIQVSQFIQFKSRTTDLYNRLRSRIRTSIPIEH-IARPQEFSKALYTFDIGQND 180

Query: 181 LGYGYQHTSEEQVRASIPDILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYNSSP 240
           L YGYQH+SEEQVRASIPDIL  F EAVQQLYKEGARYFWVHNTGPIGCL Y ILYN SP
Sbjct: 181 LAYGYQHSSEEQVRASIPDILDAFCEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP 240

Query: 241 HDRDSNGCVKSQNKVAQEFNWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGF 300
            +RDSNGCVKSQN V++EFN QLK+ LLKLGKKLP  RI  VD+YS KYLLI+ AK+ GF
Sbjct: 241 ENRDSNGCVKSQNTVSREFNRQLKSQLLKLGKKLPFARIIHVDVYSVKYLLITKAKTQGF 300

Query: 301 VNN---------------------------------PSRHITWDGIHYSEAANLWIANHI 347
           V N                                 PSRHI+WDGIHYSEAANLWIANHI
Sbjct: 301 VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCEDPSRHISWDGIHYSEAANLWIANHI 360

BLAST of Sed0023117 vs. ExPASy TrEMBL
Match: A0A6J1BYX6 (GDSL esterase/lipase At5g14450 OS=Momordica charantia OX=3673 GN=LOC111006734 PE=3 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 2.9e-151
Identity = 275/386 (71.24%), Postives = 302/386 (78.24%), Query Frame = 0

Query: 1   MDPVKRRRLLVVLILGQTVAFICAAAGG--------CGFPAVYNFGDSNSDTGGVSAAMK 60
           M P+K R +LV+LILG     + ++ GG        CGFPAVYNFGDSNSDTGG+SAA  
Sbjct: 1   MCPLKLRPVLVLLILGLVGRPVASSGGGGGRGGSGSCGFPAVYNFGDSNSDTGGISAAFN 60

Query: 61  GIQPPNGETFFGHPAGRLCDGRLIVDFIAEKLKLPYLSTYLDSLSTNFRHGANFATAGSS 120
            IQ PNGETFFGHP+GR CDGRLI+DFIAEKLKLPYLS YLDS+ T+FRHGANFAT GSS
Sbjct: 61  VIQSPNGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSVGTSFRHGANFATGGSS 120

Query: 121 IRPGGYNPFHLGIQVSQFIQFKSRTTDLYNRLRSNNRTPLPIKRSIARPWEFPKALYMFD 180
           IRPGGY+PFHLGIQVSQFIQFKSRTT+LY +LRSNNRT LPIK SIARP EF KALY FD
Sbjct: 121 IRPGGYSPFHLGIQVSQFIQFKSRTTNLYYQLRSNNRTSLPIKSSIARPGEFSKALYTFD 180

Query: 181 IGQNDLGYGYQHTSEEQVRASIPDILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPIL 240
           I QNDL YGYQH+SEEQVRASIPDIL+TFSEAVQQLYKEGARYFWVHNTGP+GCL + IL
Sbjct: 181 IAQNDLSYGYQHSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPLGCLPFNIL 240

Query: 241 YNSSPHDRDSNGCVKSQNKVAQEFNWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNA 300
           YN +P++ DS GCVKSQN+VA+E NWQLKNLLL L K LPL RIT VDM+SAKY LISNA
Sbjct: 241 YNKNPNNLDSIGCVKSQNEVARELNWQLKNLLLNLKKMLPLARITHVDMHSAKYQLISNA 300

Query: 301 KSLGFVNN---------------------------------PSRHITWDGIHYSEAANLW 346
           K+ GFVNN                                 PSRHI+WDGIHYSEAANLW
Sbjct: 301 KTQGFVNNPVKFCCGSYYGFHINCGKKEVVNGTVYGSPCKDPSRHISWDGIHYSEAANLW 360

BLAST of Sed0023117 vs. ExPASy TrEMBL
Match: A0A1S4E275 (GDSL esterase/lipase At3g27950-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498068 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.7e-151
Identity = 274/382 (71.73%), Postives = 301/382 (78.80%), Query Frame = 0

Query: 1   MDPVKRRRLLVVLIL---GQTVAFICAAAGGCGFPAVYNFGDSNSDTGGVSAAMKGIQPP 60
           M+ +K RRL  +LIL   G+TV    AAAG CGFPA+YNFGDSNSDTGG+SAA+  IQPP
Sbjct: 1   MNSLKLRRLWALLILGLVGRTVTLFGAAAGTCGFPAIYNFGDSNSDTGGISAALNAIQPP 60

Query: 61  NGETFFGHPAGRLCDGRLIVDFIAEKLKLPYLSTYLDSLSTNFRHGANFATAGSSIRPGG 120
           NGETFFGHP+GR CDGRLI+DFIAEKLKLPYLS YLDS+ T+FRHGANFAT GSSIRPGG
Sbjct: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSIGTSFRHGANFATGGSSIRPGG 120

Query: 121 YNPFHLGIQVSQFIQFKSRTTDLYNRLRSNNRTPLPIKRSIARPWEFPKALYMFDIGQND 180
           Y+PFHLGIQVSQFIQFKSRTTDL+NRLRS+NRT +PIK  IARP EF KALY FDIGQND
Sbjct: 121 YSPFHLGIQVSQFIQFKSRTTDLFNRLRSHNRTAIPIKH-IARPQEFSKALYTFDIGQND 180

Query: 181 LGYGYQHTSEEQVRASIPDILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYNSSP 240
           L YGYQH+SEEQVRASIPDIL TF EAVQQLYKEGARYFWVHNTGPIGCL Y ILYN SP
Sbjct: 181 LAYGYQHSSEEQVRASIPDILDTFCEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP 240

Query: 241 HDRDSNGCVKSQNKVAQEFNWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGF 300
            +RDS+GCVKSQN V++EFN QLK+ LLKL KKLP  RI  VDMYS KYLLI+ AKS GF
Sbjct: 241 ENRDSSGCVKSQNTVSREFNRQLKSQLLKLRKKLPFARIVHVDMYSVKYLLITKAKSQGF 300

Query: 301 VNN---------------------------------PSRHITWDGIHYSEAANLWIANHI 347
           V N                                 PSR I+WDGIHYSEAAN WIANHI
Sbjct: 301 VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCEDPSRQISWDGIHYSEAANSWIANHI 360

BLAST of Sed0023117 vs. ExPASy TrEMBL
Match: A0A6J1JTD2 (GDSL esterase/lipase At3g27950-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489560 PE=3 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 4.9e-151
Identity = 277/381 (72.70%), Postives = 299/381 (78.48%), Query Frame = 0

Query: 1   MDPVKRRRLLVVLILGQTVAFICAA--AGGCGFPAVYNFGDSNSDTGGVSAAMKGIQPPN 60
           M  +K RRLLV+ ILG  +    AA  +G CGFPAVYNFGDSNSDTGG+SAA   IQPPN
Sbjct: 1   MGLLKLRRLLVLRILGLLIGVTTAAGGSGNCGFPAVYNFGDSNSDTGGISAAFNAIQPPN 60

Query: 61  GETFFGHPAGRLCDGRLIVDFIAEKLKLPYLSTYLDSLSTNFRHGANFATAGSSIRPGGY 120
           GETFF  P+GR CDGRLIVDFIAEKLKLP+LS YLDSL TNFRHGANFAT GSSI PGGY
Sbjct: 61  GETFFRRPSGRACDGRLIVDFIAEKLKLPFLSAYLDSLGTNFRHGANFATGGSSICPGGY 120

Query: 121 NPFHLGIQVSQFIQFKSRTTDLYNRLRSNNRTPLPIKRSIARPWEFPKALYMFDIGQNDL 180
           +PFHLGIQVSQFIQFKSR TDL+NRL SNNRT   I R IARP EF KALY FDIGQNDL
Sbjct: 121 SPFHLGIQVSQFIQFKSRITDLFNRLHSNNRTSTSINR-IARPQEFSKALYTFDIGQNDL 180

Query: 181 GYGYQHTSEEQVRASIPDILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYNSSPH 240
            YGYQH+SEEQVRASIPDI++TFSEAVQQLYKEGARYFWVHNTGPIGCL Y ILYN SP 
Sbjct: 181 AYGYQHSSEEQVRASIPDIINTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPD 240

Query: 241 DRDSNGCVKSQNKVAQEFNWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGFV 300
           +RDSNGCV+SQN+V++EFN QLK+ LLKLGKKLPL RI  +DMYSAKYLLIS AK+ GFV
Sbjct: 241 NRDSNGCVESQNEVSREFNRQLKSRLLKLGKKLPLARIIHIDMYSAKYLLISKAKTQGFV 300

Query: 301 NN---------------------------------PSRHITWDGIHYSEAANLWIANHIM 347
            N                                 PSRHI+WDGIHYSEAANLWIANHI+
Sbjct: 301 KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSEAANLWIANHIL 360

BLAST of Sed0023117 vs. TAIR 10
Match: AT3G27950.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 339.0 bits (868), Expect = 4.6e-93
Identity = 183/367 (49.86%), Postives = 230/367 (62.67%), Query Frame = 0

Query: 10  LVVLILGQTVAFICAAAGGCGFPAVYNFGDSNSDTGGVSAAMKGIQPPNGETFFGHPAGR 69
           ++VL+LG T   + A +  C FPAV+NFGDSNSDTG +SAA+  + PPNG  FFG  AGR
Sbjct: 10  IIVLLLGFTEK-LSALSSSCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFFGRSAGR 69

Query: 70  LCDGRLIVDFIAEKLKLPYLSTYLDSLSTNFRHGANFATAGSSIRP--GGYNPFHLGIQV 129
             DGRLI+DFI E L LPYL+ YLDS+  N+RHGANFAT GS IRP    ++PFHLG QV
Sbjct: 70  HSDGRLIIDFITENLTLPYLTPYLDSVGANYRHGANFATGGSCIRPTLACFSPFHLGTQV 129

Query: 130 SQFIQFKSRTTDLYNRLRSNNRTPLPIKRSIARPWEFPKALYMFDIGQNDLGYGYQHTSE 189
           SQFI FK+RT  LYN+                   +F KALY  DIGQNDL  G+Q+ +E
Sbjct: 130 SQFIHFKTRTLSLYNQTN-----------------DFSKALYTLDIGQNDLAIGFQNMTE 189

Query: 190 EQVRASIPDILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYNSSPHDRDSNGCVK 249
           EQ++A+IP I+  F+ A++ LYKEGAR+F +HNTGP GCL Y +L       RD  GC+K
Sbjct: 190 EQLKATIPLIIENFTIALKLLYKEGARFFSIHNTGPTGCLPY-LLKAFPAIPRDPYGCLK 249

Query: 250 SQNKVAQEFNWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGFVN-------- 309
             N VA EFN QLKN + +L K+LP    T VD+YSAKY LI+ AK+LGF++        
Sbjct: 250 PLNNVAIEFNKQLKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFIDPFDYCCVG 309

Query: 310 -------------------------NPSRHITWDGIHYSEAANLWIANHIMNGSFSDPPL 342
                                    N    I+WDGIHY+E AN+ +AN I++GS SDPPL
Sbjct: 310 AIGRGMGCGKTIFLNGTELYSSSCQNRKNFISWDGIHYTETANMLVANRILDGSISDPPL 357

BLAST of Sed0023117 vs. TAIR 10
Match: AT5G14450.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 322.4 bits (825), Expect = 4.5e-88
Identity = 168/356 (47.19%), Postives = 226/356 (63.48%), Query Frame = 0

Query: 29  CGFPAVYNFGDSNSDTGGVSAAMKGIQPPNGETFFGHPAGRLCDGRLIVDFIAEKLKLPY 88
           C FPA+YNFGDSNSDTGG+SAA + I+ P G+ FF  P GR  DGRL +DFIAE+L LPY
Sbjct: 37  CTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQGFFHRPTGRDSDGRLTIDFIAERLGLPY 96

Query: 89  LSTYLDSLSTNFRHGANFATAGSSIR-------PGGYNPFHLGIQVSQFIQFKSRTTDLY 148
           LS YL+SL +NFRHGANFAT GS+IR         G +PF L +Q++QF QFK+R+  L+
Sbjct: 97  LSAYLNSLGSNFRHGANFATGGSTIRRQNETIFQYGISPFSLDMQIAQFDQFKARSALLF 156

Query: 149 NRLRSN-NRTPLPIKRSIARPWEFPKALYMFDIGQNDLGYGYQHTSEEQVRASIPDILST 208
            +++S  +R  LP      R  EF KALY FDIGQNDL  G++  S +Q++A+IPDI++ 
Sbjct: 157 TQIKSRYDREKLP------RQEEFAKALYTFDIGQNDLSVGFRTMSVDQLKATIPDIVNH 216

Query: 209 FSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYNSSPHD--RDSNGCVKSQNKVAQEFNW 268
            + AV+ +Y++G R FWVHNTGP GCL   + Y  +P     D +GCVK+QN++A EFN 
Sbjct: 217 LASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPAPGYLDKSGCVKAQNEMAMEFNR 276

Query: 269 QLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGFVN------------------- 328
           +LK  ++ L K+L    IT VD+Y+AKY ++SN K LGF N                   
Sbjct: 277 KLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKLGFANPLKVCCGYHEKYDHIWCGN 336

Query: 329 ---------------NPSRHITWDGIHYSEAANLWIANHIMNGSFSDPPLSIDKAC 341
                          NP   ++WDG+HY+EAAN  +A+  +NG  +DPP+ I +AC
Sbjct: 337 KGKVNNTEIYGGSCPNPVMAVSWDGVHYTEAANKHVADRTLNGLLTDPPVPITRAC 386

BLAST of Sed0023117 vs. TAIR 10
Match: AT1G67830.1 (alpha-fucosidase 1 )

HSP 1 Score: 278.5 bits (711), Expect = 7.4e-75
Identity = 154/360 (42.78%), Postives = 207/360 (57.50%), Query Frame = 0

Query: 29  CGFPAVYNFGDSNSDTGGVSAAMKGIQPPNGETFFGHPAGRLCDGRLIVDFIAEKLKLPY 88
           C FPA++NFGDSNSDTGG+SAA     PP+G +FFG PAGR CDGRL++DFIAE L LPY
Sbjct: 26  CHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSPAGRYCDGRLVIDFIAESLGLPY 85

Query: 89  LSTYLDSLSTNFRHGANFATAG-------SSIRPGGYNPFHLGIQVSQFIQFKSRTTDLY 148
           LS +LDS+ +NF HGANFATAG       S++R  G++PF L +Q  QF  F +R+  + 
Sbjct: 86  LSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFSLDVQFVQFYNFHNRSQTVR 145

Query: 149 NRLRSNNRTPLPIKRSIARPWEFPKALYMFDIGQNDLGYGY-QHTSEEQVRASIPDILST 208
           +R     +T LP   S      F KALY FDIGQNDL  GY  + + EQV   +P+I+S 
Sbjct: 146 SR-GGVYKTMLPESDS------FSKALYTFDIGQNDLTAGYFANKTVEQVETEVPEIISQ 205

Query: 209 FSEAVQQLYKEGARYFWVHNTGPIGCLHYPI-LYNSSPHDRDSNGCVKSQNKVAQEFNWQ 268
           F  A++ +Y +G RYFW+HNTGPIGCL Y I  + +   D DS+GCV   N +AQ+FN  
Sbjct: 206 FMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHGCVSPLNHLAQQFNHA 265

Query: 269 LKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGF---------------------- 328
           LK  +++L   L    IT VD+YS K+ L  +A+  GF                      
Sbjct: 266 LKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCGHGGKYNYNKGIGC 325

Query: 329 ----------------VNNPSRHITWDGIHYSEAANLWIANHIMNGSFSDPPLSIDKACQ 342
                            + P + + WDG+H+++AAN +I + I  G        + KAC+
Sbjct: 326 GMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKIAPG--------LSKACK 370

BLAST of Sed0023117 vs. TAIR 10
Match: AT3G26430.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 2.1e-74
Identity = 150/379 (39.58%), Postives = 217/379 (57.26%), Query Frame = 0

Query: 9   LLVVLILGQTVAFICAAAGGCGFPAVYNFGDSNSDTGGVSAAMKGIQPPNGETFFGHPAG 68
           L+  ++L   +    A +  C FPA++NFGDSNSDTGG+SA+      PNG+TFF  P+G
Sbjct: 7   LVKCVLLASCLIHPRACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQTFFHSPSG 66

Query: 69  RLCDGRLIVDFIAEKLKLPYLSTYLDSLSTNFRHGANFATAGSSIRP-------GGYNPF 128
           R  DGRLI+DFIAE+L LPYL+ +LDS+ +NF HGANFATAGS++RP        G +P 
Sbjct: 67  RFSDGRLIIDFIAEELGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATIAQSGVSPI 126

Query: 129 HLGIQVSQFIQFKSRTTDLYNRLRSNNRTPLPIKRSIARPWEFPKALYMFDIGQNDLGYG 188
            L +Q+ QF  F +R+  + NR           K+ + +   F +ALY FDIGQNDL  G
Sbjct: 127 SLDVQLVQFSDFITRSQLIRNR-------GGVFKKLLPKKEYFSQALYTFDIGQNDLTAG 186

Query: 189 YQ-HTSEEQVRASIPDILSTFSEAVQQLYKEGARYFWVHNTGPIGCLHYPILYNSSPHDR 248
            + + + +Q++A IPD+    S  ++++Y +G R FW+HNT P+GCL Y +     P  +
Sbjct: 187 LKLNMTSDQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPASQ 246

Query: 249 -DSNGCVKSQNKVAQEFNWQLKNLLLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGF-- 308
            D++GC   +N++A+ +N +LK  +++L K+L     T VD+YS K  LI+ AK LGF  
Sbjct: 247 IDNHGCAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGFRY 306

Query: 309 ------------------------------------VNNPSRHITWDGIHYSEAANLWIA 341
                                                N+ S  ++WDGIH++E  N WI 
Sbjct: 307 PLVACCGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSWIF 366

BLAST of Sed0023117 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 260.0 bits (663), Expect = 2.7e-69
Identity = 143/350 (40.86%), Postives = 198/350 (56.57%), Query Frame = 0

Query: 31  FPAVYNFGDSNSDTGG-VSAAMKGIQPPNGETFFGHPAGRLCDGRLIVDFIAEKLKLPYL 90
           +PA+ NFGDSNSDTG  +SA ++ + PP G+T+F  P+GR CDGRLIVDF+ +++ LP+L
Sbjct: 30  YPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNLPSGRYCDGRLIVDFLLDEMDLPFL 89

Query: 91  STYLDSLS-TNFRHGANFATAGSSI---RPGGYNPFHLGIQVSQFIQFKSRTTDLYNRLR 150
           + YLDSL   NF+ G NFA AGS+I    P   +PF   +Q+SQFI+FKSR  +L ++  
Sbjct: 90  NPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDLQISQFIRFKSRAIELLSKTG 149

Query: 151 SNNRTPLPIKRSIARPWE-FPKALYMFDIGQNDLGYGYQHTSEEQVRASIPDILSTFSEA 210
                 LP       P + + K LYM DIGQND+   +   + +QV ASIP IL TF   
Sbjct: 150 RKYEKYLP-------PIDYYSKGLYMIDIGQNDIAGAFYSKTLDQVLASIPSILETFEAG 209

Query: 211 VQQLYKEGARYFWVHNTGPIGCLHYPIL-YNSSPHDRDSNGCVKSQNKVAQEFNWQLKNL 270
           +++LY+EG R  W+HNTGP+GCL   I  + +     D  GCV S N+ A+ FN QL  +
Sbjct: 210 LKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSSHNQAAKLFNLQLHAM 269

Query: 271 LLKLGKKLPLVRITLVDMYSAKYLLISNAKSLGF-------------------------- 330
             K   + P   +T VD++S K  LI+N    GF                          
Sbjct: 270 SNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCGVGGAPLNYDSRITCGQT 329

Query: 331 ------------VNNPSRHITWDGIHYSEAANLWIANHIMNGSFSDPPLS 336
                        N+ S +I WDGIHY+EAAN ++++ I+ G +SDPP S
Sbjct: 330 KVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKYSDPPFS 372