Sed0023030 (gene) Chayote v1

Overview
NameSed0023030
Typegene
OrganismSechium edule (Chayote v1)
DescriptionTranscription factor GRAS
LocationLG01: 35446122 .. 35447799 (+)
RNA-Seq ExpressionSed0023030
SyntenySed0023030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTCCCTCCCTTTTCTTGTTCCTCATCTCTTCCATTGTCCTTTTAGAATTCACTCCTTAAAGAATTGATTCTAATTCAAATTTCCCATAAAAATTTCAGATCGCTTATCATCCAAACTGTAAAATCACCGATGGCTTTGGCCGTCGATGACCCTTTTAACGACATAGCTCTCTCCAATTGCAGCACCTCCGACGACTACCACAACCTCGCCGGAAACTGGAACTACTGGTCGCCGGTCGTCGATTGGGAGACCTTCCCACGCACCCACGACGACTTCCACGACCTTTTAGACTCCATCATGGCTGTCGACGCTCCACCATATACGGCGGAGGTCAATGGCCGCAGCAACTGCAACTCCGACTTCAGTCTGACGGAGGAAGAAGAAGAAACAAAAACAGAGGATTATACGGGGCTCCGCCTCTACCACCTCCTCACGGCGGCCGCCGACGCCTTGTTTGGCGTCAACAAAAGCCACGATTTGGCTCACGTGATATTGGTTCGTCTCAACGAATTGGCTTCACCTTCACACGGGACTAATCTGGAACGCCTCACAGCCTATTACGCCCATGCTTTACAGGGCATACTCGATAGTGCCGCCGGAGGTTCGAATAAAACCTACCACCATTATCTCAGCCGCGACGACCATACTCCGGCCCACGTGCTCGCGGCGTTTCAATTGCTGCATGAGATGTCGCCATACGTGAAATTCGGCCATTTCACTGCAAATCAAGCGATTCTCGAAGCCGTCGCCGGCGACCGGAGAGTTCACATAGTGGATTACGATATAATGGAAGGGATTCAATGGGCGTCGTTAATGCAAGCTTTTATGTCCAGAAAAGACGGTTTACCAGCACCCTATTTAAGAATCACCGCCATTTCCAGAGGCGGTGGCGGTGGAGTCAACAACCGGAGATTGATTGGCACAGTGCAGGAAACAGGCCGGCGACTGGTGGCGTTTGCGGCTTCAATTAATCAGCCATTTTCGTTTCATCACTGTAAATTGGACTCCGATGAGAGTTTTCGTCCATCTGGGTTGAAATTGGTAAGAGGAGAAGCGATTATAGTGAACTGTATGCTTCATCTTCCACATTTCAGTTGCCGGTCGCCGGAATCCACTGTCTCTTTTTTATCCGGCACAAAAACGTTGAATCCAAGGCTGGTGACTTTGGTCGAAGAGGAAATCGAGCATGGACCCACCATGGACGGCGATTACAAGGTCCAATTCCTCGATTCCTTGGAGCGATACTCGGCGATTTATGATTCACTCGAAGCAGGCTTTCAGATGAAAAACAGAGCAAGGGCATTGGTGGAGAGAGTTTTCATCGGGCCAAGAATCGCCGCTGCCGTAACCCGAATCGGACAGCCGCGTACGGCGGAAGATACAAACTCTTCGTGGGGAGATCGATTGGAGAAGATGGGATTCAAGGCGGCGCCGATCAGCTTTGCAAATCACTGCCAAGCAAGGTTGCTGGTTGATTTGTTCAACGATGGATACAGAGTTGAAGAATTGGCAAACAATAAACTGACTTTGGGATGGAAATCAAAGCGTTTGCTTTCAGCTTGCATCTGGGTTTCTTTTCCTTCTTCTTCTTCATCATCCTCTTTTTCAGATTTCGATTAGATCTGTAATTCTTTACGATTGTTCCTCTTGTTCTTTATTTCTCTCTGTTTTTTTCG

mRNA sequence

CATTCCCTCCCTTTTCTTGTTCCTCATCTCTTCCATTGTCCTTTTAGAATTCACTCCTTAAAGAATTGATTCTAATTCAAATTTCCCATAAAAATTTCAGATCGCTTATCATCCAAACTGTAAAATCACCGATGGCTTTGGCCGTCGATGACCCTTTTAACGACATAGCTCTCTCCAATTGCAGCACCTCCGACGACTACCACAACCTCGCCGGAAACTGGAACTACTGGTCGCCGGTCGTCGATTGGGAGACCTTCCCACGCACCCACGACGACTTCCACGACCTTTTAGACTCCATCATGGCTGTCGACGCTCCACCATATACGGCGGAGGTCAATGGCCGCAGCAACTGCAACTCCGACTTCAGTCTGACGGAGGAAGAAGAAGAAACAAAAACAGAGGATTATACGGGGCTCCGCCTCTACCACCTCCTCACGGCGGCCGCCGACGCCTTGTTTGGCGTCAACAAAAGCCACGATTTGGCTCACGTGATATTGGTTCGTCTCAACGAATTGGCTTCACCTTCACACGGGACTAATCTGGAACGCCTCACAGCCTATTACGCCCATGCTTTACAGGGCATACTCGATAGTGCCGCCGGAGGTTCGAATAAAACCTACCACCATTATCTCAGCCGCGACGACCATACTCCGGCCCACGTGCTCGCGGCGTTTCAATTGCTGCATGAGATGTCGCCATACGTGAAATTCGGCCATTTCACTGCAAATCAAGCGATTCTCGAAGCCGTCGCCGGCGACCGGAGAGTTCACATAGTGGATTACGATATAATGGAAGGGATTCAATGGGCGTCGTTAATGCAAGCTTTTATGTCCAGAAAAGACGGTTTACCAGCACCCTATTTAAGAATCACCGCCATTTCCAGAGGCGGTGGCGGTGGAGTCAACAACCGGAGATTGATTGGCACAGTGCAGGAAACAGGCCGGCGACTGGTGGCGTTTGCGGCTTCAATTAATCAGCCATTTTCGTTTCATCACTGTAAATTGGACTCCGATGAGAGTTTTCGTCCATCTGGGTTGAAATTGGTAAGAGGAGAAGCGATTATAGTGAACTGTATGCTTCATCTTCCACATTTCAGTTGCCGGTCGCCGGAATCCACTGTCTCTTTTTTATCCGGCACAAAAACGTTGAATCCAAGGCTGGTGACTTTGGTCGAAGAGGAAATCGAGCATGGACCCACCATGGACGGCGATTACAAGGTCCAATTCCTCGATTCCTTGGAGCGATACTCGGCGATTTATGATTCACTCGAAGCAGGCTTTCAGATGAAAAACAGAGCAAGGGCATTGGTGGAGAGAGTTTTCATCGGGCCAAGAATCGCCGCTGCCGTAACCCGAATCGGACAGCCGCGTACGGCGGAAGATACAAACTCTTCGTGGGGAGATCGATTGGAGAAGATGGGATTCAAGGCGGCGCCGATCAGCTTTGCAAATCACTGCCAAGCAAGGTTGCTGGTTGATTTGTTCAACGATGGATACAGAGTTGAAGAATTGGCAAACAATAAACTGACTTTGGGATGGAAATCAAAGCGTTTGCTTTCAGCTTGCATCTGGGTTTCTTTTCCTTCTTCTTCTTCATCATCCTCTTTTTCAGATTTCGATTAGATCTGTAATTCTTTACGATTGTTCCTCTTGTTCTTTATTTCTCTCTGTTTTTTTCG

Coding sequence (CDS)

ATGGCTTTGGCCGTCGATGACCCTTTTAACGACATAGCTCTCTCCAATTGCAGCACCTCCGACGACTACCACAACCTCGCCGGAAACTGGAACTACTGGTCGCCGGTCGTCGATTGGGAGACCTTCCCACGCACCCACGACGACTTCCACGACCTTTTAGACTCCATCATGGCTGTCGACGCTCCACCATATACGGCGGAGGTCAATGGCCGCAGCAACTGCAACTCCGACTTCAGTCTGACGGAGGAAGAAGAAGAAACAAAAACAGAGGATTATACGGGGCTCCGCCTCTACCACCTCCTCACGGCGGCCGCCGACGCCTTGTTTGGCGTCAACAAAAGCCACGATTTGGCTCACGTGATATTGGTTCGTCTCAACGAATTGGCTTCACCTTCACACGGGACTAATCTGGAACGCCTCACAGCCTATTACGCCCATGCTTTACAGGGCATACTCGATAGTGCCGCCGGAGGTTCGAATAAAACCTACCACCATTATCTCAGCCGCGACGACCATACTCCGGCCCACGTGCTCGCGGCGTTTCAATTGCTGCATGAGATGTCGCCATACGTGAAATTCGGCCATTTCACTGCAAATCAAGCGATTCTCGAAGCCGTCGCCGGCGACCGGAGAGTTCACATAGTGGATTACGATATAATGGAAGGGATTCAATGGGCGTCGTTAATGCAAGCTTTTATGTCCAGAAAAGACGGTTTACCAGCACCCTATTTAAGAATCACCGCCATTTCCAGAGGCGGTGGCGGTGGAGTCAACAACCGGAGATTGATTGGCACAGTGCAGGAAACAGGCCGGCGACTGGTGGCGTTTGCGGCTTCAATTAATCAGCCATTTTCGTTTCATCACTGTAAATTGGACTCCGATGAGAGTTTTCGTCCATCTGGGTTGAAATTGGTAAGAGGAGAAGCGATTATAGTGAACTGTATGCTTCATCTTCCACATTTCAGTTGCCGGTCGCCGGAATCCACTGTCTCTTTTTTATCCGGCACAAAAACGTTGAATCCAAGGCTGGTGACTTTGGTCGAAGAGGAAATCGAGCATGGACCCACCATGGACGGCGATTACAAGGTCCAATTCCTCGATTCCTTGGAGCGATACTCGGCGATTTATGATTCACTCGAAGCAGGCTTTCAGATGAAAAACAGAGCAAGGGCATTGGTGGAGAGAGTTTTCATCGGGCCAAGAATCGCCGCTGCCGTAACCCGAATCGGACAGCCGCGTACGGCGGAAGATACAAACTCTTCGTGGGGAGATCGATTGGAGAAGATGGGATTCAAGGCGGCGCCGATCAGCTTTGCAAATCACTGCCAAGCAAGGTTGCTGGTTGATTTGTTCAACGATGGATACAGAGTTGAAGAATTGGCAAACAATAAACTGACTTTGGGATGGAAATCAAAGCGTTTGCTTTCAGCTTGCATCTGGGTTTCTTTTCCTTCTTCTTCTTCATCATCCTCTTTTTCAGATTTCGATTAG

Protein sequence

MALAVDDPFNDIALSNCSTSDDYHNLAGNWNYWSPVVDWETFPRTHDDFHDLLDSIMAVDAPPYTAEVNGRSNCNSDFSLTEEEEETKTEDYTGLRLYHLLTAAADALFGVNKSHDLAHVILVRLNELASPSHGTNLERLTAYYAHALQGILDSAAGGSNKTYHHYLSRDDHTPAHVLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIVDYDIMEGIQWASLMQAFMSRKDGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVAFAASINQPFSFHHCKLDSDESFRPSGLKLVRGEAIIVNCMLHLPHFSCRSPESTVSFLSGTKTLNPRLVTLVEEEIEHGPTMDGDYKVQFLDSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQPRTAEDTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLFNDGYRVEELANNKLTLGWKSKRLLSACIWVSFPSSSSSSSFSDFD
Homology
BLAST of Sed0023030 vs. NCBI nr
Match: XP_038884439.1 (protein NODULATION SIGNALING PATHWAY 2 [Benincasa hispida])

HSP 1 Score: 699.9 bits (1805), Expect = 1.6e-197
Identity = 371/503 (73.76%), Postives = 406/503 (80.72%), Query Frame = 0

Query: 1   MALAVDDPFNDIALSNCST----SDDYHNLAGNWNYWSPVVDWETFPRTHDDFHDLLDSI 60
           MALA+D+ FN+I LSN ST    SDD+ +LAGNWNY SP+VDWETFP TH+DFHDL+DS+
Sbjct: 23  MALAMDNLFNEITLSNYSTSTNNSDDHRHLAGNWNYGSPIVDWETFPDTHNDFHDLVDSM 82

Query: 61  MAVDAPPYTAEVNGRSNCNSDFSLTEEEEETKTEDYTGLRLYHLLTAAADALFGVNKSHD 120
           + +D PP               S TE+ EE   ED+ GLRLYHLL AAADA+FG +KS D
Sbjct: 83  ICIDNPP---------------SPTEDREE--EEDFKGLRLYHLLVAAADAVFGDHKSRD 142

Query: 121 LAHVILVRLNELASPSHGTNLERLTAYYAHALQGILDSAAGGSNKTYH--HYLSRDDHTP 180
           LAHVILVRLNEL SPSHGTNL+RLTAYYA A Q +LDS    SNKT+H  H+L RDDHTP
Sbjct: 143 LAHVILVRLNELVSPSHGTNLQRLTAYYAQAFQDLLDSTP-VSNKTHHHNHHLHRDDHTP 202

Query: 181 AHVLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIVDYDIMEGIQWASLMQAFMS 240
             VLAAFQLL EMSPYVKF HFTANQAILEAVA DRRVHIVDYDIMEGIQWASLMQAF+S
Sbjct: 203 TDVLAAFQLLQEMSPYVKFSHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVS 262

Query: 241 RKDGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVAFAASINQPFSFHHCKLDSD 300
                 AP+LRITAISR    G N RR IGTVQETGRRLVAFAASI QPFSFH CKLDSD
Sbjct: 263 SPS---APHLRITAISR----GANGRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSD 322

Query: 301 ESFRPSGLKLVRGEAIIVNCMLHLPHFSCRSPESTVSFLSGTKTLNPRLVTLVEEEIEHG 360
           ESFRPSGLKLV+GEA++VNC+LHLPHFS RSPES  SFLSGTK+LNPR+VTLVEEEI HG
Sbjct: 323 ESFRPSGLKLVKGEALVVNCILHLPHFSYRSPESIGSFLSGTKSLNPRIVTLVEEEIGHG 382

Query: 361 PTMDGDYKVQFLDSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQPRT 420
           PT+D DYKVQFLDSLERYSAIYDSLEA   MKNRARALVERVF+GPRI+A + RIGQ R 
Sbjct: 383 PTIDVDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVERVFLGPRISATLRRIGQRRW 442

Query: 421 A---EDTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLFNDGYRVEELANNKLTLGWKS 480
           A    + N  WG++LEKMG K A ISFANHCQARLL+ LFNDGYRVEEL NNKL LGWKS
Sbjct: 443 ATAGAEENCLWGEKLEKMGLKPATISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKS 500

Query: 481 KRLLSACIWVSFPSSSSSSSFSD 495
           KRLLSA IWVS   SSSSSS SD
Sbjct: 503 KRLLSASIWVSSSLSSSSSSLSD 500

BLAST of Sed0023030 vs. NCBI nr
Match: XP_008445682.1 (PREDICTED: nodulation-signaling pathway 2 protein [Cucumis melo] >KAA0063953.1 nodulation-signaling pathway 2 protein [Cucumis melo var. makuwa] >TYK05186.1 nodulation-signaling pathway 2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 690.6 bits (1781), Expect = 9.6e-195
Identity = 365/504 (72.42%), Postives = 403/504 (79.96%), Query Frame = 0

Query: 1   MALAVDDPFNDIALSNCST----SDDYHNLAGNWNYWSPVVDWETFPRTHDDFHDLLDSI 60
           MALA+++PFNDIALSN ST    SDD+ +LAGNWNY SP+VDWETF  TH+DF D+ DS 
Sbjct: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60

Query: 61  MAVDAPPYTAEVNGRSNCNSDFSLTEEEEETKTEDYTGLRLYHLLTAAADALFGVNKSHD 120
           + ++ PP+               LTE+ +E   +++ GLRLYHLL AAADA+FG ++S D
Sbjct: 61  ICIENPPF---------------LTEDRDE--EDEFKGLRLYHLLIAAADAVFGDHRSCD 120

Query: 121 LAHVILVRLNELASPSHGTNLERLTAYYAHALQGILDSAAGGSNKTY---HHYLSRDDHT 180
           LAHVILVRLNEL SPSHGTNLERLTAYYA A Q +LDSA   SNKT+   HH   RDDHT
Sbjct: 121 LAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAP-VSNKTHHHNHHIHHRDDHT 180

Query: 181 PAHVLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIVDYDIMEGIQWASLMQAFM 240
           P  VLAAFQLL EMSPYVKFGHFTANQAILEAVA DRRVHIVDYDIMEGIQWASLMQAF+
Sbjct: 181 PTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFV 240

Query: 241 SRKDGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVAFAASINQPFSFHHCKLDS 300
           S      AP+LRITAISR    G N RR IGTVQETGRRLVAFAASI QPFSFH CKLDS
Sbjct: 241 SSPS---APHLRITAISR----GANGRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDS 300

Query: 301 DESFRPSGLKLVRGEAIIVNCMLHLPHFSCRSPESTVSFLSGTKTLNPRLVTLVEEEIEH 360
           DESFRPSGLKLV+GEA++VNCMLHLPHFS RSPES  SFLSG K+L+PR+VTLVEEEI H
Sbjct: 301 DESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGH 360

Query: 361 GPTMDGDYKVQFLDSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQPR 420
           GPT+DGDYKVQFLDSLERYSAIYDSLEA   MKNRARALVERVF+GPRI+A + RIGQ R
Sbjct: 361 GPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVERVFLGPRISATLRRIGQRR 420

Query: 421 ---TAEDTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLFNDGYRVEELANNKLTLGWK 480
               A + N  WG++LEKMG K   ISFANHCQARLL+ LFNDGYRVEEL NNKL LGWK
Sbjct: 421 WSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWK 479

Query: 481 SKRLLSACIWVSFPSSSSSSSFSD 495
           SKRLLS  IW S  SSSSSSS SD
Sbjct: 481 SKRLLSVSIWGSSTSSSSSSSLSD 479

BLAST of Sed0023030 vs. NCBI nr
Match: XP_004140976.1 (protein NODULATION SIGNALING PATHWAY 2 [Cucumis sativus] >KGN45971.1 hypothetical protein Csa_004902 [Cucumis sativus])

HSP 1 Score: 679.1 bits (1751), Expect = 2.9e-191
Identity = 364/504 (72.22%), Postives = 402/504 (79.76%), Query Frame = 0

Query: 1   MALAVDDPFNDIALSNCST----SDDYHNLAGNWNYWSPVVDWETFPRTHDDFHDLLDSI 60
           MALA+++P  DIALSN ST    SDD+ +LAGNWNY SP+VDWETF  TH+DF D+ DS 
Sbjct: 1   MALAINNPPKDIALSNYSTSTNNSDDHCHLAGNWNYGSPIVDWETFSGTHNDFLDVFDST 60

Query: 61  MAVDAPPYTAEVNGRSNCNSDFSLTEEEEETKTEDYTGLRLYHLLTAAADALFGVNKSHD 120
           + ++ PP+               LTE+ +E   +++ GLRLYHLLTAAADA+ G +KS D
Sbjct: 61  ICIENPPF---------------LTEDRDE--EDEFKGLRLYHLLTAAADAVLGDHKSCD 120

Query: 121 LAHVILVRLNELASPSHGTNLERLTAYYAHALQGILDSAAGGSNKTY---HHYLSRDDHT 180
           LAHVILVRLNEL SPSHGTNLERLTAYYA A Q +LDSA   +NKT+   HH   RDDHT
Sbjct: 121 LAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAP-VANKTHHHNHHIHQRDDHT 180

Query: 181 PAHVLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIVDYDIMEGIQWASLMQAFM 240
           P  VLAAFQLL EMSPYVKFGHFTANQAILEAVA DRRVHIVDYDIMEGIQWASLMQAF+
Sbjct: 181 PTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFV 240

Query: 241 SRKDGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVAFAASINQPFSFHHCKLDS 300
           S      AP+LRITAISR    G N RR IGTVQETGRRLVAFAASI QPFSFH CKLDS
Sbjct: 241 SSPS---APHLRITAISR----GANGRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDS 300

Query: 301 DESFRPSGLKLVRGEAIIVNCMLHLPHFSCRSPESTVSFLSGTKTLNPRLVTLVEEEIEH 360
           DESFRPSGLKLV+GEA++VNCMLHLPHFS RSPES  SFLSG K+L+PR+VTLVEEEI H
Sbjct: 301 DESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGH 360

Query: 361 GPTMDGDYKVQFLDSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQPR 420
           GPT+DGDYKVQFLDSLERYSAIYDSLEA   MKNRARALVERVF+GPRI+A + RIGQ R
Sbjct: 361 GPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVERVFLGPRISATLRRIGQRR 420

Query: 421 --TAE-DTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLFNDGYRVEELANNKLTLGWK 480
             TAE + N  WG++LEKMG K   ISFANHCQARLL+ LFNDGYRVEEL NNKL LGWK
Sbjct: 421 WSTAEMEDNCLWGEKLEKMGLKTVKISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWK 476

Query: 481 SKRLLSACIWVSFPSSSSSSSFSD 495
           SKRLLS  IW    SSSSSSS SD
Sbjct: 481 SKRLLSVSIW---GSSSSSSSLSD 476

BLAST of Sed0023030 vs. NCBI nr
Match: XP_023551167.1 (nodulation-signaling pathway 2 protein isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 672.9 bits (1735), Expect = 2.1e-189
Identity = 356/500 (71.20%), Postives = 398/500 (79.60%), Query Frame = 0

Query: 1   MALAVDDPFNDIALSNCSTSDDYHNLAGNWNYWSPVVDWETFPRTHDDFHDLLDSIMAVD 60
           MA+AVD+ FN +A+S+ STS +                      + DDFHDL DSIM++D
Sbjct: 1   MAMAVDNAFNGVAVSDYSTSTN---------------------NSDDDFHDLFDSIMSID 60

Query: 61  APPYT---AEVNGRSNCNSDFSLTEEEEETKTEDYTGLRLYHLLTAAADALFGVNKSHDL 120
           A PY+    EV+G +NCNS  +  E+ EE + E++  LRLYHLL A ADA+FG +KS DL
Sbjct: 61  ALPYSPQVVEVDGGTNCNSPSNPAEDREE-EEEEFKSLRLYHLLMAVADAMFGDHKSRDL 120

Query: 121 AHVILVRLNELASPSHGTNLERLTAYYAHALQGILDSAA---GGSNKTYHHYLSRDDHTP 180
           A VIL+RLNEL S SHGTNLERLTAYYA + Q +LD AA   GGSNK   H+L RDDH+P
Sbjct: 121 AQVILIRLNELVSTSHGTNLERLTAYYAQSFQDLLDYAAVSGGGSNKP--HHLHRDDHSP 180

Query: 181 AHVLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIVDYDIMEGIQWASLMQAFMS 240
             VLAAFQLL EMSPYVKFGHFTANQAILEAVA DRRVHIVDY+IMEGIQWASLMQA +S
Sbjct: 181 TDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQWASLMQALVS 240

Query: 241 RKDGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVAFAASINQPFSFHHCKLDSD 300
           RKD  PAP+LRITAISR    G ++RR IGTVQETGRRLVAFAASI QPFSFH CKLDSD
Sbjct: 241 RKDSPPAPHLRITAISR----GPSSRRWIGTVQETGRRLVAFAASIGQPFSFHQCKLDSD 300

Query: 301 ESFRPSGLKLVRGEAIIVNCMLHLPHFSCRSPESTVSFLSGTKTLNPRLVTLVEEEIEHG 360
           ESFRPSGLKLVRGEA++VNC+LHLPHFS  SPES  SFL+G KTLNPRLVTLVEEEIEHG
Sbjct: 301 ESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIDSFLAGAKTLNPRLVTLVEEEIEHG 360

Query: 361 PTMDGDYKVQFLDSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQPR- 420
           PT+DGDYKVQFLDSLERYSAIYDSLEAG  MKNRAR LVE+VF+GPRI+A + RIGQP+ 
Sbjct: 361 PTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLKRIGQPQR 420

Query: 421 --TAEDTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLFNDGYRVEELANNKLTLGWKS 480
             TA + N SWG+RLEKMG KAA ISFANHCQARLLVDLFNDGYRVEEL +NKL LGWKS
Sbjct: 421 AATAAEENCSWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNKLVLGWKS 472

Query: 481 KRLLSACIWVSFPSSSSSSS 492
           KRLLS  IW S   SSSSSS
Sbjct: 481 KRLLSVSIWASSSLSSSSSS 472

BLAST of Sed0023030 vs. NCBI nr
Match: XP_022993952.1 (nodulation-signaling pathway 2 protein [Cucurbita maxima])

HSP 1 Score: 671.8 bits (1732), Expect = 4.6e-189
Identity = 353/498 (70.88%), Postives = 394/498 (79.12%), Query Frame = 0

Query: 1   MALAVDDPFNDIALSNCSTSDDYHNLAGNWNYWSPVVDWETFPRTHDDFHDLLDSIMAVD 60
           MA+AVD+ FN +A+SN STS +                      + DDFHDL DSIM++D
Sbjct: 1   MAMAVDNAFNGVAVSNYSTSTN---------------------NSDDDFHDLFDSIMSID 60

Query: 61  APPYT---AEVNGRSNCNSDFSLTEEEEETKTEDYTGLRLYHLLTAAADALFGVNKSHDL 120
           A PY+    EV+G +NCNS  +  E+ EE + E++  LRLYHLL A ADA+FG NKS +L
Sbjct: 61  ALPYSPQVVEVDGGTNCNSPSNPAEDREE-EEEEFKSLRLYHLLMAVADAMFGDNKSREL 120

Query: 121 AHVILVRLNELASPSHGTNLERLTAYYAHALQGILDSAA-GGSNKTYHHYLSRDDHTPAH 180
           A VIL+RLNEL S SHGTNLERLTAYYA A Q +LD AA  G      H+L RDDH+P  
Sbjct: 121 AQVILIRLNELVSTSHGTNLERLTAYYAQAFQDLLDCAAVSGGVPNKPHHLHRDDHSPTD 180

Query: 181 VLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIVDYDIMEGIQWASLMQAFMSRK 240
           VLAAFQLL +MSPYVKFGHFTANQAILEAVA DRRVHIVDY+IMEGIQWASLMQAF+SRK
Sbjct: 181 VLAAFQLLQDMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQWASLMQAFVSRK 240

Query: 241 DGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVAFAASINQPFSFHHCKLDSDES 300
           D  PAP+LRIT ISR    G ++RR IGTVQETGRRLVAFAASI QPFSFH CKLDSDES
Sbjct: 241 DSPPAPHLRITVISR----GPSSRRWIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDES 300

Query: 301 FRPSGLKLVRGEAIIVNCMLHLPHFSCRSPESTVSFLSGTKTLNPRLVTLVEEEIEHGPT 360
           FRPSGLKLVRGEA++VNC+LHLPHFS  SPES  SFL+G KTLNPRLVTLVEEEIEHGPT
Sbjct: 301 FRPSGLKLVRGEALVVNCVLHLPHFSNSSPESIASFLAGAKTLNPRLVTLVEEEIEHGPT 360

Query: 361 MDGDYKVQFLDSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQPR--- 420
           +DGDYKVQFLDSLERYSAIYDSLEAG  MKNRAR LVE+VF+GPRI+A + RIGQP+   
Sbjct: 361 IDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLRRIGQPQRAA 420

Query: 421 TAEDTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLFNDGYRVEELANNKLTLGWKSKR 480
           TA + N  WG+RLEKMG KAA ISFANHCQARLLVDLFNDGYRVEEL +NKL LGWKSKR
Sbjct: 421 TAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNKLVLGWKSKR 472

Query: 481 LLSACIWVSFPSSSSSSS 492
           LLS  IW S  SSSSSSS
Sbjct: 481 LLSVSIWASSSSSSSSSS 472

BLAST of Sed0023030 vs. ExPASy Swiss-Prot
Match: Q5NE24 (Protein NODULATION SIGNALING PATHWAY 2 OS=Medicago truncatula OX=3880 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 4.4e-142
Identity = 275/514 (53.50%), Postives = 354/514 (68.87%), Query Frame = 0

Query: 7   DPFNDI------ALSNCSTSDDYHNLAGNWNYWSPVVDWETFPRTHDDFHDLLDSIMAVD 66
           D  ND+      +L+N  TSD+ +     WN+WSP+V+W+TF    DDFH L+D+I+  D
Sbjct: 7   DAINDLHFSGHSSLTNTPTSDEDYGC--TWNHWSPIVNWDTFTGAPDDFHHLMDTIIE-D 66

Query: 67  APPYTAEVNGRSNCNSDFSLT--EEEEETKT---------------EDYTGLRLYHLLTA 126
                 +++      +  + T  EEEEE +T               +D  GL+L HLL A
Sbjct: 67  RTTVLEQLSPSITTTTTTTTTTDEEEEEMETTTTTTTTAIKTHEVGDDSKGLKLVHLLMA 126

Query: 127 AADALFGVNKSHDLAHVILVRLNELASP-SHGTNLERLTAYYAHALQGILDSAAGGSNKT 186
            A+AL G  K+ DLA VIL+RL EL S  ++G+N+ERL A++  AL G+L+ A G  N  
Sbjct: 127 GAEALTGSTKNRDLARVILIRLKELVSQHANGSNMERLAAHFTEALHGLLEGAGGAHNNH 186

Query: 187 YH-----HYLSRD--DHTPAHVLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIV 246
           +H     HYL+ +         LAAFQLL +MSPYVKFGHFTANQAI+EAVA +RRVH++
Sbjct: 187 HHHNNNKHYLTTNGPHDNQNDTLAAFQLLQDMSPYVKFGHFTANQAIIEAVAHERRVHVI 246

Query: 247 DYDIMEGIQWASLMQAFMSRKDGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVA 306
           DYDIMEG+QWASL+Q+  S  +G   P+LRITA+SR G G    RR I TVQETGRRL +
Sbjct: 247 DYDIMEGVQWASLIQSLASNNNG---PHLRITALSRTGTG----RRSIATVQETGRRLTS 306

Query: 307 FAASINQPFSFHHCKLDSDESFRPSGLKLVRGEAIIVNCMLHLPHFSCRSPESTVSFLSG 366
           FAAS+ QPFSFHHC+LDSDE+FRPS LKLVRGEA++ NCML+LPH S R+PES  SFL+G
Sbjct: 307 FAASLGQPFSFHHCRLDSDETFRPSALKLVRGEALVFNCMLNLPHLSYRAPESVASFLNG 366

Query: 367 TKTLNPRLVTLVEEEIEHGPTMDGDYKVQFLDSLERYSAIYDSLEAGFQMKNRARALVER 426
            KTLNP+LVTLVEEE+    ++ G +  +F+DSL  YSA++DSLEAGF M+NRAR LVER
Sbjct: 367 AKTLNPKLVTLVEEEV---GSVIGGFVERFMDSLHHYSAVFDSLEAGFPMQNRARTLVER 426

Query: 427 VFIGPRIAAAVTRIGQPRTAEDTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLFNDGY 486
           VF GPRIA ++ RI +    E+   SWG+ L ++GF+  P+SFANHCQA+LL+ LFNDGY
Sbjct: 427 VFFGPRIAGSLGRIYR-TGGEEERRSWGEWLGEVGFRGVPVSFANHCQAKLLLGLFNDGY 486

Query: 487 RVEE--LANNKLTLGWKSKRLLSACIWVSFPSSS 488
           RVEE  + +NKL L WKS+RLLSA +W    S S
Sbjct: 487 RVEEVGVGSNKLVLDWKSRRLLSASLWTCSSSDS 506

BLAST of Sed0023030 vs. ExPASy Swiss-Prot
Match: Q2PEG7 (Protein NODULATION SIGNALING PATHWAY 2 OS=Lotus japonicus OX=34305 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 4.1e-140
Identity = 267/489 (54.60%), Postives = 335/489 (68.51%), Query Frame = 0

Query: 14  LSNCSTSDDYHNLAGNWNYWSPVVDWETFPRTHDDFHDLLDSIMAVD--APPYTAEVNGR 73
           L+N  +SD+  N   +WN+WSPVV+W+ F    DDFH L+DS++  +   P ++      
Sbjct: 19  LTNTPSSDN-DNYGCSWNHWSPVVNWDAFTGNQDDFHHLIDSMIDDNNTGPAFS------ 78

Query: 74  SNCNSDFSLTEEEEETKT------------------EDYTGLRLYHLLTAAADALFGVNK 133
            +  S  S  EEEEE  T                  +D+ GLRL HLL A A+AL G NK
Sbjct: 79  DHTASTTSEEEEEEEATTTTMTTTTTTTTTTPEAADDDFKGLRLVHLLMAGAEALTGANK 138

Query: 134 SHDLAHVILVRLNELASPSHGTNLERLTAYYAHALQGILDSAAGGSN-KTYHHYLSRDDH 193
           + +LA VILVRL EL S + GTN+ERL AY+  ALQG+L+ A G  N  + HH +    H
Sbjct: 139 NRELARVILVRLKELVSHTDGTNMERLAAYFTEALQGLLEGAGGAYNSSSKHHVIGGPHH 198

Query: 194 TPAH-VLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIVDYDIMEGIQWASLMQA 253
            P +  LAAFQLL +MSPYVKFGHFTANQAI+EAVA +RRVHIVDYDIMEG+QWASLMQA
Sbjct: 199 EPQNDALAAFQLLQDMSPYVKFGHFTANQAIVEAVAHERRVHIVDYDIMEGVQWASLMQA 258

Query: 254 FMSRKDGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVAFAASINQPFSFHHCKL 313
             S  +G   P+LRITA+SR G G    RR + TVQETGRRL AFA S+ QPFSFHH +L
Sbjct: 259 LASNPNG---PHLRITALSRSGVG----RRSMATVQETGRRLTAFATSLGQPFSFHHSRL 318

Query: 314 DSDESFRPSGLKLVRGEAIIVNCMLHLPHFSCRSPESTVSFLSGTKTLNPRLVTLVEEEI 373
           +SDE+FRP+GLKLVRGEA++ NCML+LPH + RSP S  SFL+  K L PRLVT+VEEE+
Sbjct: 319 ESDETFRPAGLKLVRGEALVFNCMLNLPHLTYRSPNSVASFLTAAKALRPRLVTVVEEEV 378

Query: 374 EHGPTMDGDYKVQFLDSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQ 433
             G  + G +  +F+DSL  +SA++DSLEAGF M+ RARALVERVF+GPRI  ++ RI +
Sbjct: 379 --GSAL-GGFVERFMDSLHHFSAVFDSLEAGFPMQGRARALVERVFLGPRIVGSLARIYR 438

Query: 434 PRTAEDTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLFNDGYRVEELANNKLTLGWKS 481
                +   SW + L   GF    +S ANHCQ+ LL+ LFNDGYRVEEL +NKL L WK+
Sbjct: 439 TGGGGEERGSWREWLRAAGFSGVAVSSANHCQSNLLLGLFNDGYRVEELGSNKLVLHWKT 490

BLAST of Sed0023030 vs. ExPASy Swiss-Prot
Match: Q84Q92 (Protein NODULATION SIGNALING PATHWAY 2 OS=Oryza sativa subsp. japonica OX=39947 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.2e-115
Identity = 251/544 (46.14%), Postives = 319/544 (58.64%), Query Frame = 0

Query: 1   MALAVDDPFNDIALSNC---------STSDDYHNLAGNWNYWSPVVDWETFPRTHD---- 60
           M + ++D   D   S C         S+ DD   +   W   SPV DW  F  + D    
Sbjct: 1   MEVTMEDVAGDFEFSGCGSTTTTSSASSLDDGTGMCYAWGELSPVADWANFCCSDDDGGH 60

Query: 61  DFHDLLDSIMAVD---------------APPYTAEVNGRSNCNSDFSLTEE-------EE 120
           D H L++S++  D               A  +  ++    N ++  S T         ++
Sbjct: 61  DLHGLIESMLCDDTLVGVDDDGQAGLHHADMFRDDLYCYGNGSNPSSTTTTNPGSPVFDD 120

Query: 121 ETKTEDYTGLRLYHLLTAAADALFGVNKSHDLAHVILVRLNELAS-----PSHGTNLERL 180
            T+     GLRL HLL AAA+AL G +KS +LA VILVRL E+ S      +  +N+ERL
Sbjct: 121 PTQGCPEKGLRLLHLLMAAAEALSGPHKSRELARVILVRLKEMVSHTASANAAASNMERL 180

Query: 181 TAYYAHALQGILDSA--AGGSNKTYHHYLSRDDHTPAHVLAAFQLLHEMSPYVKFGHFTA 240
            A++  ALQG+LD +   GGS +      +   H    VL AFQ+L +MSPY+KFGHFTA
Sbjct: 181 AAHFTDALQGLLDGSHPVGGSGR--QAAAAASHHHAGDVLTAFQMLQDMSPYMKFGHFTA 240

Query: 241 NQAILEAVAGDRRVHIVDYDIMEGIQWASLMQAFMSRKDGLPAPYLRITAISRGGGGGVN 300
           NQAILEAV+GDRRVHIVDYDI EGIQWASLMQA  SR DG+PAP+LRITA+SR GGGG  
Sbjct: 241 NQAILEAVSGDRRVHIVDYDIAEGIQWASLMQAMTSRADGVPAPHLRITAVSRSGGGGAR 300

Query: 301 NRRLIGTVQETGRRLVAFAASINQPFSFHHCKLDSDESFRPSGLKLVRGEAIIVNCMLHL 360
                  VQE GRRL AFAASI QPFSF  C+LDSDE FRP+ +++V+GEA++ NC+LH 
Sbjct: 301 ------AVQEAGRRLSAFAASIGQPFSFGQCRLDSDERFRPATVRMVKGEALVANCVLHQ 360

Query: 361 PHFSC---RSPESTVSFLSGTKTLNPRLVTLVEEEIEHGPTMDGD---------YKVQFL 420
              +    R   S  SFLSG   L  +LVT+VEEE E     DGD         +  QF+
Sbjct: 361 AAATTTIRRPTGSVASFLSGMAALGAKLVTVVEEEGEAEKDDDGDSAGDAAAGGFVRQFM 420

Query: 421 DSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQPRTAEDTNSSWGDRL 480
           + L RYSA++DSLEAGF  ++R R LVERV + P IA AV+R  +    E     WG  +
Sbjct: 421 EELHRYSAVWDSLEAGFPTQSRVRGLVERVILAPNIAGAVSRAYRGVDGEG-RCGWGQWM 480

Query: 481 EKMGFKAAPISFANHCQARLLVDLFNDGYRVEELANNKLTLGWKSKRLLSACIWVSFPSS 491
              GF A P+S  NH QARLL+ LFNDGY VEE   NK+ LGWK++RL+SA +W   P  
Sbjct: 481 RGSGFTAVPLSCFNHSQARLLLGLFNDGYTVEETGPNKIVLGWKARRLMSASVWAPPPLP 535

BLAST of Sed0023030 vs. ExPASy Swiss-Prot
Match: Q9SUF5 (Scarecrow-like protein 26 OS=Arabidopsis thaliana OX=3702 GN=SCL26 PE=2 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.7e-114
Identity = 230/459 (50.11%), Postives = 297/459 (64.71%), Query Frame = 0

Query: 37  VDWETFPRTHDDFHDLLDSIMA---------VDAPPYTAEVNGRSN-CNSDFSLTEEEE- 96
           +DW+       DF D+++SIM           +A P   +  G  N  ++  S+ +    
Sbjct: 38  IDWDC------DFRDVIESIMGDEGAMMEPESEAVPMLHDQEGLCNSASTGLSVADGVSF 97

Query: 97  -ETKTEDYTGLRLYHLLTAAADALFGVNKSHDLAHVILVRLNELASPSHGTNLERLTAYY 156
            E KT++  GLRL HLL AAADA  G NKS +L  VIL RL +L SP   TN+ERL A++
Sbjct: 98  GEPKTDESKGLRLVHLLVAAADASTGANKSRELTRVILARLKDLVSPGDRTNMERLAAHF 157

Query: 157 AHALQGILDSAAGGSNKTYHHYLSRDD-HTPAHVLAAFQLLHEMSPYVKFGHFTANQAIL 216
            + L  +L+      +        RDD +  A V++AF+LL  MSPYV FG+ TA QAIL
Sbjct: 158 TNGLSKLLE-----RDSVLCPQQHRDDVYDQADVISAFELLQNMSPYVNFGYLTATQAIL 217

Query: 217 EAVAGDRRVHIVDYDIMEGIQWASLMQAFMSRKDGLPAPYLRITAISRGGGGGVNNRRLI 276
           EAV  +RR+HIVDYDI EG+QWASLMQA +SR  G  A +LRITA+SR      N ++ +
Sbjct: 218 EAVKYERRIHIVDYDINEGVQWASLMQALVSRNTGPSAQHLRITALSR----ATNGKKSV 277

Query: 277 GTVQETGRRLVAFAASINQPFSFHHCKLDSDESFRPSGLKLVRGEAIIVNCMLHLPHFSC 336
             VQETGRRL AFA SI QPFS+ HCKLD++ +F  S LKLVRGEA+++NCMLHLP FS 
Sbjct: 278 AAVQETGRRLTAFADSIGQPFSYQHCKLDTN-AFSTSSLKLVRGEAVVINCMLHLPRFSH 337

Query: 337 RSPESTVSFLSGTKTLNPRLVTLVEEEIEHGPTMDGDYKVQFLDSLERYSAIYDSLEAGF 396
           ++P S +SFLS  KTLNP+LVTLV EE+  G   +  +  +F+D L ++SAI+DSLEAG 
Sbjct: 338 QTPSSVISFLSEAKTLNPKLVTLVHEEV--GLMGNQGFLYRFMDLLHQFSAIFDSLEAGL 397

Query: 397 QMKNRARALVERVFIGPRIAAAVTRIGQPRTAEDTNSSWGDRLEKMGFKAAPISFANHCQ 456
            + N AR  VERVFIGP +A  +TRI       ++ +SW   LE  GFK   +SF N CQ
Sbjct: 398 SIANPARGFVERVFIGPWVANWLTRITANDAEVESFASWPQWLETNGFKPLEVSFTNRCQ 457

Query: 457 ARLLVDLFNDGYRVEELANNKLTLGWKSKRLLSACIWVS 483
           A+LL+ LFNDG+RVEEL  N L LGWKS+RL+SA  W S
Sbjct: 458 AKLLLSLFNDGFRVEELGQNGLVLGWKSRRLVSASFWAS 478

BLAST of Sed0023030 vs. ExPASy Swiss-Prot
Match: A2ZAX5 (Protein SCARECROW 1 OS=Oryza sativa subsp. indica OX=39946 GN=SCR1 PE=3 SV=2)

HSP 1 Score: 203.8 bits (517), Expect = 4.7e-51
Identity = 134/401 (33.42%), Postives = 212/401 (52.87%), Query Frame = 0

Query: 82  EEEEETKTEDYTGLRLYHLLTAAADALFGVNKSHDLAHVILVRLNELASPSHGTNLERLT 141
           +EE+  K  D  GL L  LL   A+++   N   D AH  L+ + ELA+P  GT+ +R+ 
Sbjct: 276 KEEQRRKQRDEEGLHLLTLLLQCAESVNADNL--DEAHRALLEIAELATP-FGTSTQRVA 335

Query: 142 AYYAHALQGILDSAAGGSNKTYHHYLSRDDHTPAHVLAAFQLLHEMSPYVKFGHFTANQA 201
           AY+A A+   L S+  G      +           V AAFQ+ + +SP+VKF HFTANQA
Sbjct: 336 AYFAEAMSARLVSSCLGLYAPLPNPSPAAARLHGRVAAAFQVFNGISPFVKFSHFTANQA 395

Query: 202 ILEAVAGDRRVHIVDYDIMEGIQWASLMQAFMSRKDGLPAPYLRITAISRGGGGGVNNRR 261
           I EA   + RVHI+D DIM+G+QW  L     SR  G   P +R+T +            
Sbjct: 396 IQEAFEREERVHIIDLDIMQGLQWPGLFHILASRPGG--PPRVRLTGLGAS--------- 455

Query: 262 LIGTVQETGRRLVAFAASINQPFSFHHCKL-DSDESFRPSGLKLVRGEAIIVNCMLHLPH 321
            +  ++ TG+RL  FA ++  PF F  C + D   +  P  L + R EA+ V+ + H  +
Sbjct: 456 -MEALEATGKRLSDFADTLGLPFEF--CPVADKAGNLDPEKLGVTRREAVAVHWLRHSLY 515

Query: 322 FSCRSPESTVSFLSGTKTLNPRLVTLVEEEIEHGPTMDGDYKVQFLDSLERYSAIYDSLE 381
               S  +T+  +   + L P++VT+VE+++ H     G +  +F++++  YSA++DSL+
Sbjct: 516 DVTGSDSNTLWLI---QRLAPKVVTMVEQDLSH----SGSFLARFVEAIHYYSALFDSLD 575

Query: 382 AGFQMKNRARALVERVFIGPRIAAAVTRIGQPRTAEDTNSSWGDRLEKMGFKAAPISFAN 441
           A +   +  R +VE+  +   I   +   G  RT +    SW ++L + GF+ + ++ + 
Sbjct: 576 ASYSEDSPERHVVEQQLLSREIRNVLAVGGPARTGDVKFGSWREKLAQSGFRVSSLAGSA 635

Query: 442 HCQARLLVDLF-NDGYRVEELANNKLTLGWKSKRLLSACIW 481
             QA LL+ +F +DGY + E  N  L LGWK   LL+A  W
Sbjct: 636 AAQAALLLGMFPSDGYTLIE-ENGALKLGWKDLCLLTASAW 651

BLAST of Sed0023030 vs. ExPASy TrEMBL
Match: A0A5A7V767 (Nodulation-signaling pathway 2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1415G00580 PE=3 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 4.6e-195
Identity = 365/504 (72.42%), Postives = 403/504 (79.96%), Query Frame = 0

Query: 1   MALAVDDPFNDIALSNCST----SDDYHNLAGNWNYWSPVVDWETFPRTHDDFHDLLDSI 60
           MALA+++PFNDIALSN ST    SDD+ +LAGNWNY SP+VDWETF  TH+DF D+ DS 
Sbjct: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60

Query: 61  MAVDAPPYTAEVNGRSNCNSDFSLTEEEEETKTEDYTGLRLYHLLTAAADALFGVNKSHD 120
           + ++ PP+               LTE+ +E   +++ GLRLYHLL AAADA+FG ++S D
Sbjct: 61  ICIENPPF---------------LTEDRDE--EDEFKGLRLYHLLIAAADAVFGDHRSCD 120

Query: 121 LAHVILVRLNELASPSHGTNLERLTAYYAHALQGILDSAAGGSNKTY---HHYLSRDDHT 180
           LAHVILVRLNEL SPSHGTNLERLTAYYA A Q +LDSA   SNKT+   HH   RDDHT
Sbjct: 121 LAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAP-VSNKTHHHNHHIHHRDDHT 180

Query: 181 PAHVLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIVDYDIMEGIQWASLMQAFM 240
           P  VLAAFQLL EMSPYVKFGHFTANQAILEAVA DRRVHIVDYDIMEGIQWASLMQAF+
Sbjct: 181 PTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFV 240

Query: 241 SRKDGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVAFAASINQPFSFHHCKLDS 300
           S      AP+LRITAISR    G N RR IGTVQETGRRLVAFAASI QPFSFH CKLDS
Sbjct: 241 SSPS---APHLRITAISR----GANGRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDS 300

Query: 301 DESFRPSGLKLVRGEAIIVNCMLHLPHFSCRSPESTVSFLSGTKTLNPRLVTLVEEEIEH 360
           DESFRPSGLKLV+GEA++VNCMLHLPHFS RSPES  SFLSG K+L+PR+VTLVEEEI H
Sbjct: 301 DESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGH 360

Query: 361 GPTMDGDYKVQFLDSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQPR 420
           GPT+DGDYKVQFLDSLERYSAIYDSLEA   MKNRARALVERVF+GPRI+A + RIGQ R
Sbjct: 361 GPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVERVFLGPRISATLRRIGQRR 420

Query: 421 ---TAEDTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLFNDGYRVEELANNKLTLGWK 480
               A + N  WG++LEKMG K   ISFANHCQARLL+ LFNDGYRVEEL NNKL LGWK
Sbjct: 421 WSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWK 479

Query: 481 SKRLLSACIWVSFPSSSSSSSFSD 495
           SKRLLS  IW S  SSSSSSS SD
Sbjct: 481 SKRLLSVSIWGSSTSSSSSSSLSD 479

BLAST of Sed0023030 vs. ExPASy TrEMBL
Match: A0A1S3BE54 (nodulation-signaling pathway 2 protein OS=Cucumis melo OX=3656 GN=LOC103488636 PE=3 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 4.6e-195
Identity = 365/504 (72.42%), Postives = 403/504 (79.96%), Query Frame = 0

Query: 1   MALAVDDPFNDIALSNCST----SDDYHNLAGNWNYWSPVVDWETFPRTHDDFHDLLDSI 60
           MALA+++PFNDIALSN ST    SDD+ +LAGNWNY SP+VDWETF  TH+DF D+ DS 
Sbjct: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60

Query: 61  MAVDAPPYTAEVNGRSNCNSDFSLTEEEEETKTEDYTGLRLYHLLTAAADALFGVNKSHD 120
           + ++ PP+               LTE+ +E   +++ GLRLYHLL AAADA+FG ++S D
Sbjct: 61  ICIENPPF---------------LTEDRDE--EDEFKGLRLYHLLIAAADAVFGDHRSCD 120

Query: 121 LAHVILVRLNELASPSHGTNLERLTAYYAHALQGILDSAAGGSNKTY---HHYLSRDDHT 180
           LAHVILVRLNEL SPSHGTNLERLTAYYA A Q +LDSA   SNKT+   HH   RDDHT
Sbjct: 121 LAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAP-VSNKTHHHNHHIHHRDDHT 180

Query: 181 PAHVLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIVDYDIMEGIQWASLMQAFM 240
           P  VLAAFQLL EMSPYVKFGHFTANQAILEAVA DRRVHIVDYDIMEGIQWASLMQAF+
Sbjct: 181 PTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFV 240

Query: 241 SRKDGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVAFAASINQPFSFHHCKLDS 300
           S      AP+LRITAISR    G N RR IGTVQETGRRLVAFAASI QPFSFH CKLDS
Sbjct: 241 SSPS---APHLRITAISR----GANGRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDS 300

Query: 301 DESFRPSGLKLVRGEAIIVNCMLHLPHFSCRSPESTVSFLSGTKTLNPRLVTLVEEEIEH 360
           DESFRPSGLKLV+GEA++VNCMLHLPHFS RSPES  SFLSG K+L+PR+VTLVEEEI H
Sbjct: 301 DESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGH 360

Query: 361 GPTMDGDYKVQFLDSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQPR 420
           GPT+DGDYKVQFLDSLERYSAIYDSLEA   MKNRARALVERVF+GPRI+A + RIGQ R
Sbjct: 361 GPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVERVFLGPRISATLRRIGQRR 420

Query: 421 ---TAEDTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLFNDGYRVEELANNKLTLGWK 480
               A + N  WG++LEKMG K   ISFANHCQARLL+ LFNDGYRVEEL NNKL LGWK
Sbjct: 421 WSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWK 479

Query: 481 SKRLLSACIWVSFPSSSSSSSFSD 495
           SKRLLS  IW S  SSSSSSS SD
Sbjct: 481 SKRLLSVSIWGSSTSSSSSSSLSD 479

BLAST of Sed0023030 vs. ExPASy TrEMBL
Match: A0A0A0KDX4 (GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040600 PE=3 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.4e-191
Identity = 364/504 (72.22%), Postives = 402/504 (79.76%), Query Frame = 0

Query: 1   MALAVDDPFNDIALSNCST----SDDYHNLAGNWNYWSPVVDWETFPRTHDDFHDLLDSI 60
           MALA+++P  DIALSN ST    SDD+ +LAGNWNY SP+VDWETF  TH+DF D+ DS 
Sbjct: 1   MALAINNPPKDIALSNYSTSTNNSDDHCHLAGNWNYGSPIVDWETFSGTHNDFLDVFDST 60

Query: 61  MAVDAPPYTAEVNGRSNCNSDFSLTEEEEETKTEDYTGLRLYHLLTAAADALFGVNKSHD 120
           + ++ PP+               LTE+ +E   +++ GLRLYHLLTAAADA+ G +KS D
Sbjct: 61  ICIENPPF---------------LTEDRDE--EDEFKGLRLYHLLTAAADAVLGDHKSCD 120

Query: 121 LAHVILVRLNELASPSHGTNLERLTAYYAHALQGILDSAAGGSNKTY---HHYLSRDDHT 180
           LAHVILVRLNEL SPSHGTNLERLTAYYA A Q +LDSA   +NKT+   HH   RDDHT
Sbjct: 121 LAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAP-VANKTHHHNHHIHQRDDHT 180

Query: 181 PAHVLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIVDYDIMEGIQWASLMQAFM 240
           P  VLAAFQLL EMSPYVKFGHFTANQAILEAVA DRRVHIVDYDIMEGIQWASLMQAF+
Sbjct: 181 PTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFV 240

Query: 241 SRKDGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVAFAASINQPFSFHHCKLDS 300
           S      AP+LRITAISR    G N RR IGTVQETGRRLVAFAASI QPFSFH CKLDS
Sbjct: 241 SSPS---APHLRITAISR----GANGRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDS 300

Query: 301 DESFRPSGLKLVRGEAIIVNCMLHLPHFSCRSPESTVSFLSGTKTLNPRLVTLVEEEIEH 360
           DESFRPSGLKLV+GEA++VNCMLHLPHFS RSPES  SFLSG K+L+PR+VTLVEEEI H
Sbjct: 301 DESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGH 360

Query: 361 GPTMDGDYKVQFLDSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQPR 420
           GPT+DGDYKVQFLDSLERYSAIYDSLEA   MKNRARALVERVF+GPRI+A + RIGQ R
Sbjct: 361 GPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVERVFLGPRISATLRRIGQRR 420

Query: 421 --TAE-DTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLFNDGYRVEELANNKLTLGWK 480
             TAE + N  WG++LEKMG K   ISFANHCQARLL+ LFNDGYRVEEL NNKL LGWK
Sbjct: 421 WSTAEMEDNCLWGEKLEKMGLKTVKISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWK 476

Query: 481 SKRLLSACIWVSFPSSSSSSSFSD 495
           SKRLLS  IW    SSSSSSS SD
Sbjct: 481 SKRLLSVSIW---GSSSSSSSLSD 476

BLAST of Sed0023030 vs. ExPASy TrEMBL
Match: A0A6J1JUC4 (nodulation-signaling pathway 2 protein OS=Cucurbita maxima OX=3661 GN=LOC111489805 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 2.2e-189
Identity = 353/498 (70.88%), Postives = 394/498 (79.12%), Query Frame = 0

Query: 1   MALAVDDPFNDIALSNCSTSDDYHNLAGNWNYWSPVVDWETFPRTHDDFHDLLDSIMAVD 60
           MA+AVD+ FN +A+SN STS +                      + DDFHDL DSIM++D
Sbjct: 1   MAMAVDNAFNGVAVSNYSTSTN---------------------NSDDDFHDLFDSIMSID 60

Query: 61  APPYT---AEVNGRSNCNSDFSLTEEEEETKTEDYTGLRLYHLLTAAADALFGVNKSHDL 120
           A PY+    EV+G +NCNS  +  E+ EE + E++  LRLYHLL A ADA+FG NKS +L
Sbjct: 61  ALPYSPQVVEVDGGTNCNSPSNPAEDREE-EEEEFKSLRLYHLLMAVADAMFGDNKSREL 120

Query: 121 AHVILVRLNELASPSHGTNLERLTAYYAHALQGILDSAA-GGSNKTYHHYLSRDDHTPAH 180
           A VIL+RLNEL S SHGTNLERLTAYYA A Q +LD AA  G      H+L RDDH+P  
Sbjct: 121 AQVILIRLNELVSTSHGTNLERLTAYYAQAFQDLLDCAAVSGGVPNKPHHLHRDDHSPTD 180

Query: 181 VLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIVDYDIMEGIQWASLMQAFMSRK 240
           VLAAFQLL +MSPYVKFGHFTANQAILEAVA DRRVHIVDY+IMEGIQWASLMQAF+SRK
Sbjct: 181 VLAAFQLLQDMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQWASLMQAFVSRK 240

Query: 241 DGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVAFAASINQPFSFHHCKLDSDES 300
           D  PAP+LRIT ISR    G ++RR IGTVQETGRRLVAFAASI QPFSFH CKLDSDES
Sbjct: 241 DSPPAPHLRITVISR----GPSSRRWIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDES 300

Query: 301 FRPSGLKLVRGEAIIVNCMLHLPHFSCRSPESTVSFLSGTKTLNPRLVTLVEEEIEHGPT 360
           FRPSGLKLVRGEA++VNC+LHLPHFS  SPES  SFL+G KTLNPRLVTLVEEEIEHGPT
Sbjct: 301 FRPSGLKLVRGEALVVNCVLHLPHFSNSSPESIASFLAGAKTLNPRLVTLVEEEIEHGPT 360

Query: 361 MDGDYKVQFLDSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQPR--- 420
           +DGDYKVQFLDSLERYSAIYDSLEAG  MKNRAR LVE+VF+GPRI+A + RIGQP+   
Sbjct: 361 IDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLRRIGQPQRAA 420

Query: 421 TAEDTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLFNDGYRVEELANNKLTLGWKSKR 480
           TA + N  WG+RLEKMG KAA ISFANHCQARLLVDLFNDGYRVEEL +NKL LGWKSKR
Sbjct: 421 TAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNKLVLGWKSKR 472

Query: 481 LLSACIWVSFPSSSSSSS 492
           LLS  IW S  SSSSSSS
Sbjct: 481 LLSVSIWASSSSSSSSSS 472

BLAST of Sed0023030 vs. ExPASy TrEMBL
Match: A0A6J1FK48 (nodulation-signaling pathway 2 protein OS=Cucurbita moschata OX=3662 GN=LOC111444761 PE=3 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 3.6e-187
Identity = 352/500 (70.40%), Postives = 394/500 (78.80%), Query Frame = 0

Query: 1   MALAVDDPFNDIALSNCSTSDDYHNLAGNWNYWSPVVDWETFPRTHDDFHDLLDSIMAVD 60
           MA+AVD+ FN +A+S+ STS +  N                     DDFHDL DSIM++D
Sbjct: 1   MAMAVDNAFNGVAVSDYSTSTNNSN---------------------DDFHDLFDSIMSID 60

Query: 61  APPYT---AEVNGRSNCNSDFSLTEEEEETKTEDYTGLRLYHLLTAAADALFGVNKSHDL 120
           A PY+    E++G +NCNS  +  E  EE + E++  LRLYHLL A ADA+FG +KS +L
Sbjct: 61  ALPYSPQVVEIDGGTNCNSPSNPAEGREE-EEEEFRSLRLYHLLMAVADAMFGDHKSREL 120

Query: 121 AHVILVRLNELASPSHGTNLERLTAYYAHALQGILDSAA---GGSNKTYHHYLSRDDHTP 180
           A VIL+RLNEL S SHGTNLER+TAYYA A Q +LD AA   GG NK   H+L RDDH+P
Sbjct: 121 AQVILIRLNELVSTSHGTNLERITAYYAQAFQDLLDCAAVSGGGPNKP--HHLHRDDHSP 180

Query: 181 AHVLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIVDYDIMEGIQWASLMQAFMS 240
             VLAAFQLL EMSPYVKFGHFTANQAILEAVA DRRVHIVDY+IMEGIQWASLMQA +S
Sbjct: 181 TDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQWASLMQALVS 240

Query: 241 RKDGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVAFAASINQPFSFHHCKLDSD 300
           RKD  PAP+LRITAISR    G ++RR IGTVQETGRRLVAFAASI QPFSFH CKLDSD
Sbjct: 241 RKDSPPAPHLRITAISR----GPSSRRWIGTVQETGRRLVAFAASIGQPFSFHQCKLDSD 300

Query: 301 ESFRPSGLKLVRGEAIIVNCMLHLPHFSCRSPESTVSFLSGTKTLNPRLVTLVEEEIEHG 360
           ESFRPSGLKLVRGEA++VNC+LHLPHFS  SPES  SFL+G KTLNPRLVTLVEEEIEHG
Sbjct: 301 ESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFLAGAKTLNPRLVTLVEEEIEHG 360

Query: 361 PTMDGDYKVQFLDSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQPR- 420
           PT+DGDYKVQFLDSLERYSAIYDSLEAG  MKNRAR LVE+VF+GPRI+A + RI QP+ 
Sbjct: 361 PTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLRRIEQPQR 420

Query: 421 --TAEDTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLFNDGYRVEELANNKLTLGWKS 480
             TA + N  WG+RLEKMG KAA ISFANHCQARLLVDLFNDGYRVEEL +NKL LGWKS
Sbjct: 421 AATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNKLVLGWKS 472

Query: 481 KRLLSACIWVSFPSSSSSSS 492
           KRLLS  IW S   SSSSSS
Sbjct: 481 KRLLSVSIWASSSLSSSSSS 472

BLAST of Sed0023030 vs. TAIR 10
Match: AT4G08250.1 (GRAS family transcription factor )

HSP 1 Score: 414.5 bits (1064), Expect = 1.2e-115
Identity = 230/459 (50.11%), Postives = 297/459 (64.71%), Query Frame = 0

Query: 37  VDWETFPRTHDDFHDLLDSIMA---------VDAPPYTAEVNGRSN-CNSDFSLTEEEE- 96
           +DW+       DF D+++SIM           +A P   +  G  N  ++  S+ +    
Sbjct: 38  IDWDC------DFRDVIESIMGDEGAMMEPESEAVPMLHDQEGLCNSASTGLSVADGVSF 97

Query: 97  -ETKTEDYTGLRLYHLLTAAADALFGVNKSHDLAHVILVRLNELASPSHGTNLERLTAYY 156
            E KT++  GLRL HLL AAADA  G NKS +L  VIL RL +L SP   TN+ERL A++
Sbjct: 98  GEPKTDESKGLRLVHLLVAAADASTGANKSRELTRVILARLKDLVSPGDRTNMERLAAHF 157

Query: 157 AHALQGILDSAAGGSNKTYHHYLSRDD-HTPAHVLAAFQLLHEMSPYVKFGHFTANQAIL 216
            + L  +L+      +        RDD +  A V++AF+LL  MSPYV FG+ TA QAIL
Sbjct: 158 TNGLSKLLE-----RDSVLCPQQHRDDVYDQADVISAFELLQNMSPYVNFGYLTATQAIL 217

Query: 217 EAVAGDRRVHIVDYDIMEGIQWASLMQAFMSRKDGLPAPYLRITAISRGGGGGVNNRRLI 276
           EAV  +RR+HIVDYDI EG+QWASLMQA +SR  G  A +LRITA+SR      N ++ +
Sbjct: 218 EAVKYERRIHIVDYDINEGVQWASLMQALVSRNTGPSAQHLRITALSR----ATNGKKSV 277

Query: 277 GTVQETGRRLVAFAASINQPFSFHHCKLDSDESFRPSGLKLVRGEAIIVNCMLHLPHFSC 336
             VQETGRRL AFA SI QPFS+ HCKLD++ +F  S LKLVRGEA+++NCMLHLP FS 
Sbjct: 278 AAVQETGRRLTAFADSIGQPFSYQHCKLDTN-AFSTSSLKLVRGEAVVINCMLHLPRFSH 337

Query: 337 RSPESTVSFLSGTKTLNPRLVTLVEEEIEHGPTMDGDYKVQFLDSLERYSAIYDSLEAGF 396
           ++P S +SFLS  KTLNP+LVTLV EE+  G   +  +  +F+D L ++SAI+DSLEAG 
Sbjct: 338 QTPSSVISFLSEAKTLNPKLVTLVHEEV--GLMGNQGFLYRFMDLLHQFSAIFDSLEAGL 397

Query: 397 QMKNRARALVERVFIGPRIAAAVTRIGQPRTAEDTNSSWGDRLEKMGFKAAPISFANHCQ 456
            + N AR  VERVFIGP +A  +TRI       ++ +SW   LE  GFK   +SF N CQ
Sbjct: 398 SIANPARGFVERVFIGPWVANWLTRITANDAEVESFASWPQWLETNGFKPLEVSFTNRCQ 457

Query: 457 ARLLVDLFNDGYRVEELANNKLTLGWKSKRLLSACIWVS 483
           A+LL+ LFNDG+RVEEL  N L LGWKS+RL+SA  W S
Sbjct: 458 AKLLLSLFNDGFRVEELGQNGLVLGWKSRRLVSASFWAS 478

BLAST of Sed0023030 vs. TAIR 10
Match: AT3G54220.1 (GRAS family transcription factor )

HSP 1 Score: 189.5 bits (480), Expect = 6.5e-48
Identity = 134/430 (31.16%), Postives = 222/430 (51.63%), Query Frame = 0

Query: 58  AVDAPPYTAEVNG---RSNCNSDFSLTEEEEETK--TEDYTGLRLYHLLTAAADALFGVN 117
           + DAPP    V         N+  +L E +EE K   +D  GL L  LL   A+A+   N
Sbjct: 246 STDAPPQPETVTATVPAVQTNTAEALRERKEEIKRQKQDEEGLHLLTLLLQCAEAVSADN 305

Query: 118 KSHDLAHVILVRLNELASPSHGTNLERLTAYYAHALQGILDSAAGGSNKTYHHYLSRDDH 177
                A+ +L+ +++L++P +GT+ +R+ AY++ A+   L ++  G             H
Sbjct: 306 LEE--ANKLLLEISQLSTP-YGTSAQRVAAYFSEAMSARLLNSCLGIYAALPSRWMPQTH 365

Query: 178 TPAHVLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIVDYDIMEGIQWASLMQAF 237
           +   +++AFQ+ + +SP VKF HFTANQAI EA   +  VHI+D DIM+G+QW  L    
Sbjct: 366 S-LKMVSAFQVFNGISPLVKFSHFTANQAIQEAFEKEDSVHIIDLDIMQGLQWPGLFHIL 425

Query: 238 MSRKDGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVAFAASINQPFSFHHCKL- 297
            SR  G   P++R+T +             +  +Q TG+RL  FA  +  PF F  C L 
Sbjct: 426 ASRPGG--PPHVRLTGLGTS----------MEALQATGKRLSDFADKLGLPFEF--CPLA 485

Query: 298 DSDESFRPSGLKLVRGEAIIVNCMLHLPHFSCRSPESTVSFLSGTKTLNPRLVTLVEEEI 357
           +   +     L + + EA+ V+ + H  +    S   T+  L   + L P++VT+VE+++
Sbjct: 486 EKVGNLDTERLNVRKREAVAVHWLQHSLYDVTGSDAHTLWLL---QRLAPKVVTVVEQDL 545

Query: 358 EHGPTMDGDYKVQFLDSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQ 417
            H     G +  +F++++  YSA++DSL A +  ++  R +VE+  +   I   +   G 
Sbjct: 546 SHA----GSFLGRFVEAIHYYSALFDSLGASYGEESEERHVVEQQLLSKEIRNVLAVGGP 605

Query: 418 PRTAEDTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLF-NDGYRVEELANNKLTLGWK 477
            R+ E    SW +++++ GFK   ++     QA LL+ +F +DGY + +  N  L LGWK
Sbjct: 606 SRSGEVKFESWREKMQQCGFKGISLAGNAATQATLLLGMFPSDGYTLVD-DNGTLKLGWK 649

Query: 478 SKRLLSACIW 481
              LL+A  W
Sbjct: 666 DLSLLTASAW 649

BLAST of Sed0023030 vs. TAIR 10
Match: AT5G41920.1 (GRAS family transcription factor )

HSP 1 Score: 177.6 bits (449), Expect = 2.5e-44
Identity = 128/406 (31.53%), Postives = 200/406 (49.26%), Query Frame = 0

Query: 84  EEETKTEDYTGLRLYHLLTAAADALFGVNKSHDLAHVILVRLNELASPSHGTNLERLTAY 143
           EE  + +    ++L  LL   A+  +        A  +L  ++E+ SP  G++ ER+ AY
Sbjct: 27  EETLENDGAAAIKLLSLLLQCAE--YVATDHLREASTLLSEISEICSP-FGSSPERVVAY 86

Query: 144 YAHALQ-GILDSAAGGSNKTYHHYLSRDDHTPAH---VLAAFQLLHEMSPYVKFGHFTAN 203
           +A ALQ  ++ S   G+       LS    T      + +A Q  + +SP +KF HFTAN
Sbjct: 87  FAQALQTRVISSYLSGACSP----LSEKPLTVVQSQKIFSALQTYNSVSPLIKFSHFTAN 146

Query: 204 QAILEAVAGDRRVHIVDYDIMEGIQWASLMQAFMSRKDGLPAPYLRITAISRGGGGGVNN 263
           QAI +A+ G+  VHI+D D+M+G+QW +L     SR   L +  +RIT            
Sbjct: 147 QAIFQALDGEDSVHIIDLDVMQGLQWPALFHILASRPRKLRS--IRITGFGSSS------ 206

Query: 264 RRLIGTVQETGRRLVAFAASINQPFSFHHCKLDSDESFRPSGLKLVRGEAIIVNCMLHLP 323
                 +  TGRRL  FA+S+N PF FH  +        PS L   +GEA++V+ M H  
Sbjct: 207 ----DLLASTGRRLADFASSLNLPFEFHPIEGIIGNLIDPSQLATRQGEAVVVHWMQHRL 266

Query: 324 HFSCRSPESTVSFLSGTKTLNPRLVTLVEEEIEHGPTMDGDYKVQFLDSLERYSAIYDSL 383
           +    +   T+  L   + L P L+T+VE+E+ +     G +  +F+++L  YSA++D+L
Sbjct: 267 YDVTGNNLETLEIL---RRLKPNLITVVEQELSYDD--GGSFLGRFVEALHYYSALFDAL 326

Query: 384 EAGFQMKNRARALVERVFIGPRIAAAVTRIGQPRTAEDTNSSWGDRLEKMGFKAAPISFA 443
             G   ++  R  VE++ +G  I   V   G  R        W + L ++GF+   +   
Sbjct: 327 GDGLGEESGERFTVEQIVLGTEIRNIVAHGGGRR----KRMKWKEELSRVGFRPVSLRGN 386

Query: 444 NHCQARLLVDLFN-DGYRVEELANNKLTLGWKSKRLLSACIWVSFP 485
              QA LL+ +   +GY + E  N  L LGWK   LL+A  W S P
Sbjct: 387 PATQAGLLLGMLPWNGYTLVE-ENGTLRLGWKDLSLLTASAWKSQP 403

BLAST of Sed0023030 vs. TAIR 10
Match: AT3G03450.1 (RGA-like 2 )

HSP 1 Score: 175.3 bits (443), Expect = 1.3e-43
Identity = 126/397 (31.74%), Postives = 199/397 (50.13%), Query Frame = 0

Query: 93  TGLRLYHLLTAAADALFGVNKSHDLAHVILVRLNELASPSHGTNLERLTAYYAHALQGIL 152
           TG+RL H L A A+A+   N   +LA  ++ R+  LA    G  + ++  Y+A AL    
Sbjct: 176 TGVRLVHALVACAEAIHQENL--NLADALVKRVGTLAGSQAGA-MGKVATYFAQAL---- 235

Query: 153 DSAAGGSNKTYHHYLSRDDHTPAHVLAAFQLLHEM-----SPYVKFGHFTANQAILEAVA 212
                 + + Y  Y +  D   A V  +F+ + EM      PY+KF HFTANQAILEAV 
Sbjct: 236 ------ARRIYRDYTAETD-VCAAVNPSFEEVLEMHFYESCPYLKFAHFTANQAILEAVT 295

Query: 213 GDRRVHIVDYDIMEGIQWASLMQAFMSRKDGLPAPYLRITAISRGGGGGVNNRRLIGTVQ 272
             RRVH++D  + +G+QW +LMQA   R  G P+   R+T I      G        ++Q
Sbjct: 296 TARRVHVIDLGLNQGMQWPALMQALALRPGGPPS--FRLTGI------GPPQTENSDSLQ 355

Query: 273 ETGRRLVAFAASINQPFSFHHCKLDSDESFRPSGLKL-VRGEAIIVNCMLHLPHFSCRSP 332
           + G +L  FA ++   F F     +S     P   +     E ++VN +  L     RS 
Sbjct: 356 QLGWKLAQFAQNMGVEFEFKGLAAESLSDLEPEMFETRPESETLVVNSVFELHRLLARS- 415

Query: 333 ESTVSFLSGTKTLNPRLVTLVEEEIEHGPTMDGDYKVQFLDSLERYSAIYDSLEAGFQMK 392
            S    L+  K + P +VT+VE+E  H   +  D   +F ++L  YS+++DSLE  + + 
Sbjct: 416 GSIEKLLNTVKAIKPSIVTVVEQEANHNGIVFLD---RFNEALHYYSSLFDSLEDSYSLP 475

Query: 393 NRARALVERVFIGPRIAAAVTRIGQPRT-AEDTNSSWGDRLEKMGFKAAPISFANHCQAR 452
           ++ R + E V++G +I   V   G  R    +T + W  R++  GF    +  +   QA 
Sbjct: 476 SQDRVMSE-VYLGRQILNVVAAEGSDRVERHETAAQWRIRMKSAGFDPIHLGSSAFKQAS 535

Query: 453 LLVDLF--NDGYRVEELANNKLTLGWKSKRLLSACIW 481
           +L+ L+   DGYRVEE  +  L +GW+++ L++   W
Sbjct: 536 MLLSLYATGDGYRVEE-NDGCLMIGWQTRPLITTSAW 544

BLAST of Sed0023030 vs. TAIR 10
Match: AT5G17490.1 (RGA-like protein 3 )

HSP 1 Score: 169.1 bits (427), Expect = 9.0e-42
Identity = 149/489 (30.47%), Postives = 225/489 (46.01%), Query Frame = 0

Query: 10  NDIALSNCSTSDDYHNLAGNWNYW--SPVVDWETFPRTHDDFHDLLDSIMAVDAPPYTAE 69
           NDIA S+ + +D  H    + + W  S + D   +P         LD     D  P T +
Sbjct: 65  NDIASSSNAFNDTVHYNPSDLSGWAQSMLSDLNYYPD--------LDPNRICDLRPITDD 124

Query: 70  ---VNGRSNCNS--------DFSLTEEEEETKTEDYTGLRLYHLLTAAADALFGVNKSHD 129
               +  SN N         D   +E        + TG+RL   L A A+A+   N S  
Sbjct: 125 DECCSSNSNSNKRIRLGPWCDSVTSESTRSVVLIEETGVRLVQALVACAEAVQLENLS-- 184

Query: 130 LAHVILVRLNELASPSHGTNLERLTAYYAHALQGILDSAAGGSNKTYHHYLSRDDHTPAH 189
           LA  ++ R+  LA+   G  + ++  Y+A AL          + + Y  + S     P+ 
Sbjct: 185 LADALVKRVGLLAASQAGA-MGKVATYFAEAL----------ARRIYRIHPSAAAIDPSF 244

Query: 190 VLAAFQLLHEMSPYVKFGHFTANQAILEAVAGDRRVHIVDYDIMEGIQWASLMQAFMSRK 249
                   ++  PY+KF HFTANQAILEAV   R VH++D  + +G+QW +LMQA   R 
Sbjct: 245 EEILQMNFYDSCPYLKFAHFTANQAILEAVTTSRVVHVIDLGLNQGMQWPALMQALALRP 304

Query: 250 DGLPAPYLRITAISRGGGGGVNNRRLIGTVQETGRRLVAFAASINQPFSFHHCKLDSDES 309
            G P+   R+T     G G  +NR     +QE G +L   A +I   F F+    +    
Sbjct: 305 GGPPS--FRLT-----GVGNPSNRE---GIQELGWKLAQLAQAIGVEFKFNGLTTERLSD 364

Query: 310 FRPSGLKL-VRGEAIIVNCMLHLPHFSCRSPESTVSFLSGTKTLNPRLVTLVEEEIEHGP 369
             P   +     E ++VN +  L H     P S    L+  K + P LVT+VE+E  H  
Sbjct: 365 LEPDMFETRTESETLVVNSVFEL-HPVLSQPGSIEKLLATVKAVKPGLVTVVEQEANH-- 424

Query: 370 TMDGD-YKVQFLDSLERYSAIYDSLEAGFQMKNRARALVERVFIGPRIAAAVTRIGQPR- 429
             +GD +  +F ++L  YS+++DSLE G  + ++ R + E V++G +I   V   G  R 
Sbjct: 425 --NGDVFLDRFNEALHYYSSLFDSLEDGVVIPSQDRVMSE-VYLGRQILNLVATEGSDRI 484

Query: 430 TAEDTNSSWGDRLEKMGFKAAPISFANHCQARLLVDLF--NDGYRVEELANNKLTLGWKS 481
              +T + W  R+   GF    +      QA LL+ L    DGYRVEE  +  L L W++
Sbjct: 485 ERHETLAQWRKRMGSAGFDPVNLGSDAFKQASLLLALSGGGDGYRVEE-NDGSLMLAWQT 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884439.11.6e-19773.76protein NODULATION SIGNALING PATHWAY 2 [Benincasa hispida][more]
XP_008445682.19.6e-19572.42PREDICTED: nodulation-signaling pathway 2 protein [Cucumis melo] >KAA0063953.1 n... [more]
XP_004140976.12.9e-19172.22protein NODULATION SIGNALING PATHWAY 2 [Cucumis sativus] >KGN45971.1 hypothetica... [more]
XP_023551167.12.1e-18971.20nodulation-signaling pathway 2 protein isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022993952.14.6e-18970.88nodulation-signaling pathway 2 protein [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q5NE244.4e-14253.50Protein NODULATION SIGNALING PATHWAY 2 OS=Medicago truncatula OX=3880 GN=NSP2 PE... [more]
Q2PEG74.1e-14054.60Protein NODULATION SIGNALING PATHWAY 2 OS=Lotus japonicus OX=34305 GN=NSP2 PE=1 ... [more]
Q84Q921.2e-11546.14Protein NODULATION SIGNALING PATHWAY 2 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9SUF51.7e-11450.11Scarecrow-like protein 26 OS=Arabidopsis thaliana OX=3702 GN=SCL26 PE=2 SV=1[more]
A2ZAX54.7e-5133.42Protein SCARECROW 1 OS=Oryza sativa subsp. indica OX=39946 GN=SCR1 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7V7674.6e-19572.42Nodulation-signaling pathway 2 protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3BE544.6e-19572.42nodulation-signaling pathway 2 protein OS=Cucumis melo OX=3656 GN=LOC103488636 P... [more]
A0A0A0KDX41.4e-19172.22GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040600 PE=3 S... [more]
A0A6J1JUC42.2e-18970.88nodulation-signaling pathway 2 protein OS=Cucurbita maxima OX=3661 GN=LOC1114898... [more]
A0A6J1FK483.6e-18770.40nodulation-signaling pathway 2 protein OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
Match NameE-valueIdentityDescription
AT4G08250.11.2e-11550.11GRAS family transcription factor [more]
AT3G54220.16.5e-4831.16GRAS family transcription factor [more]
AT5G41920.12.5e-4431.53GRAS family transcription factor [more]
AT3G03450.11.3e-4331.74RGA-like 2 [more]
AT5G17490.19.0e-4230.47RGA-like protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005202Transcription factor GRASPFAMPF03514GRAScoord: 98..480
e-value: 2.2E-94
score: 316.5
IPR005202Transcription factor GRASPANTHERPTHR31636OSJNBA0084A10.13 PROTEIN-RELATEDcoord: 16..482
IPR005202Transcription factor GRASPROSITEPS50985GRAScoord: 88..481
score: 67.459953
IPR030028Scarecrow-like protein 26/nodulation signalling pathway 2PANTHERPTHR31636:SF25SCARECROW-LIKE PROTEIN 26coord: 16..482

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0023030.1Sed0023030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding