Sed0022815 (gene) Chayote v1

BLAST of Sed0022815 vs. NCBI nr
Match: XP_022971834.1 (protein SHORTAGE IN CHIASMATA 1 isoform X3 [Cucurbita maxima])

HSP 1 Score: 2526.9 bits (6548), Expect = 0.0e+00
Identity = 1304/1666 (78.27%), Postives = 1429/1666 (85.77%), Query Frame = 0

Query: 1    MRTRFLDIDYFAAGNEPFRCLPVPHLASKPPLSTAGDLLHFDFLPEISLAIDRLAIDSAL 60
            MRTRFL+ID+FA GNE F  LPVPHL S  PLST GDLLHFDFLPEISL ID L+IDSA+
Sbjct: 1    MRTRFLNIDFFAPGNESFHRLPVPHLVSN-PLSTVGDLLHFDFLPEISLGIDSLSIDSAI 60

Query: 61   SKFFDDVLPRRIDDDNDYRD-----SRFQGSTERI--------RFLEEEAKCAYEDNVEG 120
            S+FFDDVLPRRI+DD+ YRD     S F GS ERI        R  EEEAK  YEDN+EG
Sbjct: 61   SRFFDDVLPRRIEDDDVYRDAGCQSSGFHGSAERIFSSESVGTRLREEEAKGTYEDNMEG 120

Query: 121  GWKNFGSETSETEFKAIGTVIRNRNLTYDMIQFETPQLDAFLENAFLFEREEVQILAEFP 180
             WKNFGSETSE EFK IGT IRNRN + DMIQFETPQLDAFLENAFLFE EEVQIL   P
Sbjct: 121  SWKNFGSETSEMEFKDIGTEIRNRNFSNDMIQFETPQLDAFLENAFLFEEEEVQILTAMP 180

Query: 181  EAKFNLETLNQGLLNCPSEVKESVYAIEGITSEYLLDQKSCLVEEDFAQDQKLLHRLTFP 240
            E +F+LETLNQGLL  PSEVKES+Y +E I SEYLLDQ+SCL E+DFAQDQKL H+ TFP
Sbjct: 181  EVEFDLETLNQGLLKYPSEVKESIYDVEIIPSEYLLDQESCLFEDDFAQDQKLSHQFTFP 240

Query: 241  FLEVDEMVLETSAFFSMQDELCFVLDNSESPHSIQDDNLLVNDEENLCSMKSDIEEFLLD 300
            FLEVDEMVLET AF S+QDEL F+L+N+ES H IQDDNLLVN+EE L  M+ D EEFL D
Sbjct: 241  FLEVDEMVLETLAFLSLQDELFFILENTESAHRIQDDNLLVNNEEYLSFMRCDAEEFLSD 300

Query: 301  HFLRPCAVSEVASPDISGG-SDLMSLMEILEIPVSSAVQTKPSCDLPVGLAIFEEFQLLD 360
            HFLRPCAVSE+ASPDISGG SDLMS+ME LEI VSS VQTK SCDL V  AIFEEFQLLD
Sbjct: 301  HFLRPCAVSELASPDISGGSSDLMSMMETLEIAVSSEVQTKSSCDLSVEPAIFEEFQLLD 360

Query: 361  IYSNQPFEAFFDLELSAVSEISDCMSIENMNFKSFNELIVCHELALVDDTFKSLPVPLLS 420
             YSNQ F   FDLE+SA+SEI+DC SIEN NFKSFNELIVCHEL LVDD FKSLPVP+LS
Sbjct: 361  TYSNQHFGVIFDLEVSAMSEITDCTSIENTNFKSFNELIVCHELVLVDDKFKSLPVPILS 420

Query: 421  SEESERPMFAFIEDVLANLNGKSLSTSDGIYLDWYLLEEDSCSFGIYSSYQNLLEEINLK 480
            S+ SE+P+ AFIEDVLANLN +SLS SDGIYLDW+LL+  S S G+YSSYQN+LEEINLK
Sbjct: 421  SQGSEKPLNAFIEDVLANLNKQSLSASDGIYLDWHLLDGSSYSSGLYSSYQNMLEEINLK 480

Query: 481  PVEFDQEPCGYDSIFYRYVFSDDALDRERTEDKGELKEPFPDGISMLPSQTIDVASSKLL 540
            PVEFD+EPC  D IFYRYVFSDDAL RERTEDKGELKE FP+GI ML  QTIDVAS KLL
Sbjct: 481  PVEFDREPCENDGIFYRYVFSDDALVRERTEDKGELKESFPEGIPMLSGQTIDVASRKLL 540

Query: 541  NDGCQQLGRQNLVAVGNTEKVLSSWKSQSELNDPKFFLNSQKPAGTRKGESVFSATDINT 600
            N+ CQQ  RQ L AVGN+EK LSSWKS+SE ND KFFLNSQKPAG RK ESVFSA D NT
Sbjct: 541  NERCQQKERQGLAAVGNSEKALSSWKSKSESNDLKFFLNSQKPAGARKSESVFSAIDTNT 600

Query: 601  ILPKVPCDGQLTNNPSTTSADVSLKQLNVTLHQVCLSDNILHLINNSEKTYLAILQNETE 660
             LPKV  DG LTN PS +SAD SLKQLNV +HQVCLSDNILHLINNSEKTYLAILQNETE
Sbjct: 601  TLPKVSRDGILTNKPSMSSADDSLKQLNVAVHQVCLSDNILHLINNSEKTYLAILQNETE 660

Query: 661  LRKTYLPYVAEDYVLMLSLPKQKLIDCVKKIYLLGTSNYWEEKVMTLATLYAIKQIAWYL 720
            LRK YLPYVAEDY+LMLSLPKQKLIDC+KKIYL GT+ YWEEK+MTLATLYA KQI WYL
Sbjct: 661  LRKKYLPYVAEDYLLMLSLPKQKLIDCIKKIYLQGTNTYWEEKIMTLATLYATKQIVWYL 720

Query: 721  CFYGIHPAHLYLKKLCQSLECLKSRLGFLASLIEDASKMVDREITISHPALTTIQDILCS 780
            CF+GIHPAHLYLKKLCQS ECLKSRLGFL S IE+A KMVD+EITISHPALTTIQ+ILCS
Sbjct: 721  CFFGIHPAHLYLKKLCQSSECLKSRLGFLVSSIEEAGKMVDKEITISHPALTTIQEILCS 780

Query: 781  KTSSSTLKVLVVANEIFWGSLKKLFGSMGLSFEELSFGGPTDQQVFNANSMVDELVPNCL 840
            +TS+S+LKVLVVAN+IFW SLK L GS+G+SF E+++G  TD+QV NAN+MVD+L  NCL
Sbjct: 781  RTSTSSLKVLVVANQIFWWSLKILLGSLGISFAEINYGSLTDEQVSNANAMVDDLASNCL 840

Query: 841  LVSQGHISGSFPFNKFSIILEYGGINGSSQISTYFTKSINMPHLHFVMLELEKCGNCKAF 900
            LVSQ +ISGSFPFNKFSI+LEYGG+NGSSQIS YF+  I+MPHLHF+MLEL+KCGNCKAF
Sbjct: 841  LVSQEYISGSFPFNKFSIVLEYGGLNGSSQISAYFSNLIDMPHLHFIMLELDKCGNCKAF 900

Query: 901  CKGVGLPQHNELKIEEKSLVENQTRMLEELLNFLPVDEKNILAPPEATIEAEDLKVPLQV 960
            C+GV  PQHNEL IEEKSLVENQT MLE+LLNFLPV+EK ILA  + TIEAE+ +VPL+ 
Sbjct: 901  CEGVDFPQHNELTIEEKSLVENQTMMLEKLLNFLPVEEKYILASSKKTIEAENRRVPLRA 960

Query: 961  SVGPVSGKSQHIDLVSFPEAIVIVNTQKFEKEMIVSRRSSYQRLLALEKDGVQVVERDLS 1020
             V PVS KSQH DLVSFPEAI+IVNTQKFEKEMIV RRSSYQR+LALEK+GVQVVERDL 
Sbjct: 961  PVVPVSDKSQHTDLVSFPEAIIIVNTQKFEKEMIVCRRSSYQRILALEKEGVQVVERDLC 1020

Query: 1021 FPVDLIITSGLCLMWYDCTNIHRKASTPNEASSCMNLCIENIATDVLTSLSFAFSGCILV 1080
             PVDLII  G+CL WYDCTNI RK ST NEAS C+NLCIENIATDVLTSLSFAF GC+LV
Sbjct: 1021 LPVDLIIAPGICLFWYDCTNIDRKGSTSNEASLCLNLCIENIATDVLTSLSFAFRGCVLV 1080

Query: 1081 FEGEISYLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKSMNRHLY 1140
            FEGEIS LSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSK M R +Y
Sbjct: 1081 FEGEISSLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKLMTRRIY 1140

Query: 1141 PKMSESETLAESFLTSFPSINPLTAHGILSSESFLCDFLEWPHERRLHAVKKYCIPDESV 1200
            PKMSESETLAESFLTSFPSINPL AHGILSSES L DFLEWPHERRLHA++KY IPD+ +
Sbjct: 1141 PKMSESETLAESFLTSFPSINPLIAHGILSSESLLADFLEWPHERRLHAIRKYSIPDDCI 1200

Query: 1201 SLFSVLCKYGEREDSKSVMTDCSSSVSSGPDSDKCYFNGNSKRKRRNFTGGTQCIDKNMD 1260
            SLFS LCKYGEREDSKSVMTDCSSS+SSG DS+KC+FNGNS+RKRRNFTGGTQCI+KN D
Sbjct: 1201 SLFSALCKYGEREDSKSVMTDCSSSLSSGADSEKCHFNGNSERKRRNFTGGTQCIEKNTD 1260

Query: 1261 VFNSDAPNPFSAGSRETLSASKSLGSQMFEDQEIFSGLKGLSSSVNHFFDQNHNLESFDA 1320
               S+  NPF+AG+ ETL ASKS GSQ+FED EIF  LKGLSSSVN+FFDQNHNL SFDA
Sbjct: 1261 CLYSNTLNPFTAGTAETLVASKSFGSQLFEDPEIFCDLKGLSSSVNNFFDQNHNLASFDA 1320

Query: 1321 SISMNPNSFGKSRDSWISTSPEISNEIRRHCSSYVQNQGLDSNRRKRHNLHNMNKSENQH 1380
            ++SM+P    K RD WIST+PEIS+EIRR CSS+VQNQGLD +++K HN HNMNKSEN H
Sbjct: 1321 TVSMDPTRVCKPRDCWISTAPEISDEIRRRCSSFVQNQGLDRSKKKIHNFHNMNKSENHH 1380

Query: 1381 EELTGEVVSLIDDPVFKDDFASMAPMNFLPSMLDYEKDSPRKSKIQRKLYHGQSDHPFSA 1440
            EEL  EV +LID+PV KD FA+MAPMNFLPSMLD E DS RKSKIQR+L +GQSDHPF A
Sbjct: 1381 EELIAEVDNLIDNPVLKDHFATMAPMNFLPSMLDNETDSSRKSKIQRRLSYGQSDHPFCA 1440

Query: 1441 VGMANNSSSDFWSPMNFHRQNSGGLDNHFPDPSFQP-----RYKDDHSDEGLMQNSVRDS 1500
            V + NNSSSDFWS +N H Q+S GLDN FPDPSF+P     RYKDDH DEGL+QN +RDS
Sbjct: 1441 VDVGNNSSSDFWSSINLHGQSSRGLDNQFPDPSFEPSIMPLRYKDDHLDEGLIQNPLRDS 1500

Query: 1501 KVLFSLSEKGMSHSDVTPLSIALRSKHLQESSPWTMEFLNRIKEKSRNRLHSVPCGSSSP 1560
            KV FSLSEK  SHSDVTPLS+ALRSKHLQESSPWTMEFLNRI+EKSRNR HSVP GSSSP
Sbjct: 1501 KVSFSLSEKDTSHSDVTPLSVALRSKHLQESSPWTMEFLNRIREKSRNRQHSVPRGSSSP 1560

Query: 1561 FPEHLGNVRKTVKRRSPSILEFFKYQGGSTQRKKPEQKRQKQSMQSSNSSKNVLVPTNEL 1620
            FPE+LGNV+KTVKRRSPSILEFFKYQGGST RKKPEQKR K S QSSNSSKNVLV T EL
Sbjct: 1561 FPENLGNVKKTVKRRSPSILEFFKYQGGSTLRKKPEQKRHKPSTQSSNSSKNVLVATREL 1620

Query: 1621 SSWTPIDKRSRQTLSFAIDGNERQTKLVWSNDNYGLGQSSQKLTNK 1648
            SS TPIDKRSRQTLS+A DGN  QTKLVWSNDNYGLG++SQKL NK
Sbjct: 1621 SSGTPIDKRSRQTLSYATDGNGSQTKLVWSNDNYGLGKNSQKLGNK 1665

BLAST of Sed0022815 vs. NCBI nr
Match: XP_023554172.1 (protein SHORTAGE IN CHIASMATA 1 isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2522.3 bits (6536), Expect = 0.0e+00
Identity = 1305/1667 (78.28%), Postives = 1429/1667 (85.72%), Query Frame = 0

Query: 1    MRTRFLDIDYFAAGNEPFRCLPVPHLASKPPLSTAGDLLHFDFLPEISLAIDRLAIDSAL 60
            MRTRFL+ID+FA GNE F  LPVPHL S  PLST GDLLHFDFLPEISL ID L+IDSA+
Sbjct: 1    MRTRFLNIDFFAPGNESFHRLPVPHLVSN-PLSTVGDLLHFDFLPEISLGIDSLSIDSAI 60

Query: 61   SKFFDDVLPRRIDDDNDYRD-----SRFQGSTERI--------RFLEEEAKCAYEDNVEG 120
            S+FFDDVLPRRI+DD+ YRD     S F GS ERI        R  E+EAK  YEDN+EG
Sbjct: 61   SRFFDDVLPRRIEDDDVYRDAGCQSSGFHGSAERIFSSGSVGTRLREQEAKGTYEDNMEG 120

Query: 121  GWKNFGSETSETEF-KAIGTVIRNRNLTYDMIQFETPQLDAFLENAFLFEREEVQILAEF 180
             WKNFGSETSE EF K IGT IRNRN + DMIQFETPQLDAFLENAFLFE+EEVQIL   
Sbjct: 121  SWKNFGSETSEMEFIKDIGTEIRNRNFSNDMIQFETPQLDAFLENAFLFEKEEVQILTGM 180

Query: 181  PEAKFNLETLNQGLLNCPSEVKESVYAIEGITSEYLLDQKSCLVEEDFAQDQKLLHRLTF 240
            PE +F+LETLNQGLL  PSEVKES+Y +E I SEYLLDQ+SCL E+DFAQDQKL H+ TF
Sbjct: 181  PEVEFDLETLNQGLLKYPSEVKESIYDVEIIPSEYLLDQESCLFEDDFAQDQKLSHQFTF 240

Query: 241  PFLEVDEMVLETSAFFSMQDELCFVLDNSESPHSIQDDNLLVNDEENLCSMKSDIEEFLL 300
            PFLEVDEMVLET AF S+QDEL F+L+N+ES H IQDDNLLVN+EE L SM+ D EEFL 
Sbjct: 241  PFLEVDEMVLETLAFLSLQDELFFILENTESAHMIQDDNLLVNNEEYLSSMRCDAEEFLS 300

Query: 301  DHFLRPCAVSEVASPDISGG-SDLMSLMEILEIPVSSAVQTKPSCDLPVGLAIFEEFQLL 360
            DHFLRPCAVSE+ASPDISGG SDLMS+ME LEI VSS VQTK SCDL V  AIFEEFQLL
Sbjct: 301  DHFLRPCAVSELASPDISGGLSDLMSMMETLEIAVSSEVQTKSSCDLSVEPAIFEEFQLL 360

Query: 361  DIYSNQPFEAFFDLELSAVSEISDCMSIENMNFKSFNELIVCHELALVDDTFKSLPVPLL 420
            D YSNQ F    DLE+SA+SEI+DC SIEN NFKSFNELIVCHEL LVDDTFKSLPVP+L
Sbjct: 361  DTYSNQHFGVILDLEVSAMSEITDCTSIENTNFKSFNELIVCHELVLVDDTFKSLPVPIL 420

Query: 421  SSEESERPMFAFIEDVLANLNGKSLSTSDGIYLDWYLLEEDSCSFGIYSSYQNLLEEINL 480
            SS+ SE+P+ AFIEDVLANLN +SLS SDGIYLDW+LL+E S S G+YSSYQN+LEEINL
Sbjct: 421  SSQGSEKPLNAFIEDVLANLNKQSLSASDGIYLDWHLLDESSYSSGLYSSYQNMLEEINL 480

Query: 481  KPVEFDQEPCGYDSIFYRYVFSDDALDRERTEDKGELKEPFPDGISMLPSQTIDVASSKL 540
            KPVEFDQEPC  D IFYRYVFSDDAL RERTEDKGELKE FP+GI ML  QTIDVASSKL
Sbjct: 481  KPVEFDQEPCENDGIFYRYVFSDDALVRERTEDKGELKESFPEGIPMLSGQTIDVASSKL 540

Query: 541  LNDGCQQLGRQNLVAVGNTEKVLSSWKSQSELNDPKFFLNSQKPAGTRKGESVFSATDIN 600
            LN+ CQQ  RQ L AVGN+EK LSSWKS+SE ND KFFLNSQKPA  RK ESVFS  D N
Sbjct: 541  LNERCQQKERQGLAAVGNSEKALSSWKSKSESNDLKFFLNSQKPARVRKSESVFSEIDTN 600

Query: 601  TILPKVPCDGQLTNNPSTTSADVSLKQLNVTLHQVCLSDNILHLINNSEKTYLAILQNET 660
            T LPKVP DG LTN PS +SAD SLKQLNV +HQVCLSDNILHLINNSEKTYLAILQNET
Sbjct: 601  TTLPKVPRDGILTNKPSMSSADDSLKQLNVAVHQVCLSDNILHLINNSEKTYLAILQNET 660

Query: 661  ELRKTYLPYVAEDYVLMLSLPKQKLIDCVKKIYLLGTSNYWEEKVMTLATLYAIKQIAWY 720
            ELRK YLPYVAEDY+LMLSLPKQ LIDC+KKIYL GT+ YWEEK+MTLATLYA KQI WY
Sbjct: 661  ELRKKYLPYVAEDYLLMLSLPKQMLIDCIKKIYLQGTNTYWEEKIMTLATLYATKQIVWY 720

Query: 721  LCFYGIHPAHLYLKKLCQSLECLKSRLGFLASLIEDASKMVDREITISHPALTTIQDILC 780
            LCFYGIHPAHLYLKKLCQS ECLKSRLGFL S IE+A KMV REITISHPALTTIQ+ILC
Sbjct: 721  LCFYGIHPAHLYLKKLCQSSECLKSRLGFLVSSIEEAGKMVGREITISHPALTTIQEILC 780

Query: 781  SKTSSSTLKVLVVANEIFWGSLKKLFGSMGLSFEELSFGGPTDQQVFNANSMVDELVPNC 840
            S+TS S+LKVLVVAN+IFW SLKKL GS+G+SF EL++G  TD+QV NAN+MVD+L  NC
Sbjct: 781  SRTSISSLKVLVVANQIFWWSLKKLLGSLGISFAELNYGSLTDEQVSNANAMVDDLASNC 840

Query: 841  LLVSQGHISGSFPFNKFSIILEYGGINGSSQISTYFTKSINMPHLHFVMLELEKCGNCKA 900
            LLVS+ +ISGSFPFNKFSI+LEYGG+NGSSQIS YF+  I+MPHLHF+MLEL+KCGNCKA
Sbjct: 841  LLVSEEYISGSFPFNKFSIVLEYGGLNGSSQISAYFSNLIDMPHLHFIMLELDKCGNCKA 900

Query: 901  FCKGVGLPQHNELKIEEKSLVENQTRMLEELLNFLPVDEKNILAPPEATIEAEDLKVPLQ 960
            FC+GV  PQHNEL IEEKSLVENQT MLE+LLNFLPV+EK ILA P+ TIEAE+ +V L+
Sbjct: 901  FCEGVDFPQHNELTIEEKSLVENQTMMLEKLLNFLPVEEKYILASPKKTIEAENRRVSLR 960

Query: 961  VSVGPVSGKSQHIDLVSFPEAIVIVNTQKFEKEMIVSRRSSYQRLLALEKDGVQVVERDL 1020
              V PVS KSQH DLVSFPEAI+IVNTQKFEKEMIV RRSSYQR+LALEK+GVQVVERDL
Sbjct: 961  APVVPVSDKSQHTDLVSFPEAIIIVNTQKFEKEMIVCRRSSYQRILALEKEGVQVVERDL 1020

Query: 1021 SFPVDLIITSGLCLMWYDCTNIHRKASTPNEASSCMNLCIENIATDVLTSLSFAFSGCIL 1080
              PVDLII  G+CL WYDCTNI RK ST NEAS  +NLCIENIATDVLTSLSFAF GC+L
Sbjct: 1021 CLPVDLIIAPGICLFWYDCTNIDRKGSTSNEASLSLNLCIENIATDVLTSLSFAFRGCVL 1080

Query: 1081 VFEGEISYLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKSMNRHL 1140
            VFEGEIS LSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSK M R +
Sbjct: 1081 VFEGEISSLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKLMTRRI 1140

Query: 1141 YPKMSESETLAESFLTSFPSINPLTAHGILSSESFLCDFLEWPHERRLHAVKKYCIPDES 1200
            YPKMSESETLAESFLTSFPSINPL AHGILSSES L DFLEWPHERRLHA++KY IPD+ 
Sbjct: 1141 YPKMSESETLAESFLTSFPSINPLIAHGILSSESLLADFLEWPHERRLHAIRKYSIPDDC 1200

Query: 1201 VSLFSVLCKYGEREDSKSVMTDCSSSVSSGPDSDKCYFNGNSKRKRRNFTGGTQCIDKNM 1260
            +SLFS LCKYGEREDSKSVMTDCSSS+SSG DS+KC+FNGNS+RKRRNFTGG+QCI+KN 
Sbjct: 1201 ISLFSALCKYGEREDSKSVMTDCSSSLSSGADSEKCHFNGNSERKRRNFTGGSQCIEKNT 1260

Query: 1261 DVFNSDAPNPFSAGSRETLSASKSLGSQMFEDQEIFSGLKGLSSSVNHFFDQNHNLESFD 1320
            D   S+  NPF+AG+ ETL ASKS GSQ+FED EIF  LKGLSSSVN+FFDQNHNL SFD
Sbjct: 1261 DCLYSNTLNPFTAGTAETLVASKSFGSQLFEDPEIFCDLKGLSSSVNNFFDQNHNLASFD 1320

Query: 1321 ASISMNPNSFGKSRDSWISTSPEISNEIRRHCSSYVQNQGLDSNRRKRHNLHNMNKSENQ 1380
            A++SM+P    K RD WIST+PEIS+EIRRHCSS+VQNQGLD +++K HN HNMNKSEN 
Sbjct: 1321 ATVSMDPTRVCKPRDCWISTAPEISDEIRRHCSSFVQNQGLDRSKKKIHNFHNMNKSENH 1380

Query: 1381 HEELTGEVVSLIDDPVFKDDFASMAPMNFLPSMLDYEKDSPRKSKIQRKLYHGQSDHPFS 1440
            HEEL  EV +LID+PV KD FA+MAPMNFLPSML+ E DS RKSKIQR+L +GQSDHPF 
Sbjct: 1381 HEELIAEVDNLIDNPVLKDHFATMAPMNFLPSMLENETDSSRKSKIQRRLSYGQSDHPFC 1440

Query: 1441 AVGMANNSSSDFWSPMNFHRQNSGGLDNHFPDPSFQP-----RYKDDHSDEGLMQNSVRD 1500
            AV + NNSSSDFWS +N H Q+S GLDN FPDPSF+P     RYKDDH DEGL QN +RD
Sbjct: 1441 AVDVGNNSSSDFWSSINLHGQSSRGLDNQFPDPSFEPSIMPLRYKDDHLDEGLTQNPLRD 1500

Query: 1501 SKVLFSLSEKGMSHSDVTPLSIALRSKHLQESSPWTMEFLNRIKEKSRNRLHSVPCGSSS 1560
            SKV FSLSEK  SHSDVTPLS+ALRSKHLQESSPWTMEFLNRI+EKSRNR HSVP GSSS
Sbjct: 1501 SKVSFSLSEKHTSHSDVTPLSVALRSKHLQESSPWTMEFLNRIREKSRNRQHSVPRGSSS 1560

Query: 1561 PFPEHLGNVRKTVKRRSPSILEFFKYQGGSTQRKKPEQKRQKQSMQSSNSSKNVLVPTNE 1620
            PFPE+LGNV+KTVKRRSPSILEFFKYQGGST RKKPEQKR K S QSSNSSKNVLV T E
Sbjct: 1561 PFPENLGNVKKTVKRRSPSILEFFKYQGGSTLRKKPEQKRHKPSTQSSNSSKNVLVATRE 1620

Query: 1621 LSSWTPIDKRSRQTLSFAIDGNERQTKLVWSNDNYGLGQSSQKLTNK 1648
            LSS TPIDKRSRQTLS+A DGN  QTKLVWSNDNYGLG++SQKL NK
Sbjct: 1621 LSSGTPIDKRSRQTLSYATDGNGSQTKLVWSNDNYGLGKNSQKLGNK 1666

BLAST of Sed0022815 vs. NCBI nr
Match: XP_022971833.1 (protein SHORTAGE IN CHIASMATA 1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 2521.9 bits (6535), Expect = 0.0e+00
Identity = 1304/1668 (78.18%), Postives = 1429/1668 (85.67%), Query Frame = 0

Query: 1    MRTRFLDIDYFAAGNEPFRCLPVPHLASKPPLSTAGDLLHFDFLPEISLAIDRLAIDSAL 60
            MRTRFL+ID+FA GNE F  LPVPHL S  PLST GDLLHFDFLPEISL ID L+IDSA+
Sbjct: 1    MRTRFLNIDFFAPGNESFHRLPVPHLVSN-PLSTVGDLLHFDFLPEISLGIDSLSIDSAI 60

Query: 61   SKFFDDVLPRRIDDDNDYRD-----SRFQGSTERI--------RFLEEEAKCAYEDNVEG 120
            S+FFDDVLPRRI+DD+ YRD     S F GS ERI        R  EEEAK  YEDN+EG
Sbjct: 61   SRFFDDVLPRRIEDDDVYRDAGCQSSGFHGSAERIFSSESVGTRLREEEAKGTYEDNMEG 120

Query: 121  GWKNFGSETSETEF--KAIGTVIRNRNLTYDMIQFETPQLDAFLENAFLFEREEVQILAE 180
             WKNFGSETSE EF  K IGT IRNRN + DMIQFETPQLDAFLENAFLFE EEVQIL  
Sbjct: 121  SWKNFGSETSEMEFVKKDIGTEIRNRNFSNDMIQFETPQLDAFLENAFLFEEEEVQILTA 180

Query: 181  FPEAKFNLETLNQGLLNCPSEVKESVYAIEGITSEYLLDQKSCLVEEDFAQDQKLLHRLT 240
             PE +F+LETLNQGLL  PSEVKES+Y +E I SEYLLDQ+SCL E+DFAQDQKL H+ T
Sbjct: 181  MPEVEFDLETLNQGLLKYPSEVKESIYDVEIIPSEYLLDQESCLFEDDFAQDQKLSHQFT 240

Query: 241  FPFLEVDEMVLETSAFFSMQDELCFVLDNSESPHSIQDDNLLVNDEENLCSMKSDIEEFL 300
            FPFLEVDEMVLET AF S+QDEL F+L+N+ES H IQDDNLLVN+EE L  M+ D EEFL
Sbjct: 241  FPFLEVDEMVLETLAFLSLQDELFFILENTESAHRIQDDNLLVNNEEYLSFMRCDAEEFL 300

Query: 301  LDHFLRPCAVSEVASPDISGG-SDLMSLMEILEIPVSSAVQTKPSCDLPVGLAIFEEFQL 360
             DHFLRPCAVSE+ASPDISGG SDLMS+ME LEI VSS VQTK SCDL V  AIFEEFQL
Sbjct: 301  SDHFLRPCAVSELASPDISGGSSDLMSMMETLEIAVSSEVQTKSSCDLSVEPAIFEEFQL 360

Query: 361  LDIYSNQPFEAFFDLELSAVSEISDCMSIENMNFKSFNELIVCHELALVDDTFKSLPVPL 420
            LD YSNQ F   FDLE+SA+SEI+DC SIEN NFKSFNELIVCHEL LVDD FKSLPVP+
Sbjct: 361  LDTYSNQHFGVIFDLEVSAMSEITDCTSIENTNFKSFNELIVCHELVLVDDKFKSLPVPI 420

Query: 421  LSSEESERPMFAFIEDVLANLNGKSLSTSDGIYLDWYLLEEDSCSFGIYSSYQNLLEEIN 480
            LSS+ SE+P+ AFIEDVLANLN +SLS SDGIYLDW+LL+  S S G+YSSYQN+LEEIN
Sbjct: 421  LSSQGSEKPLNAFIEDVLANLNKQSLSASDGIYLDWHLLDGSSYSSGLYSSYQNMLEEIN 480

Query: 481  LKPVEFDQEPCGYDSIFYRYVFSDDALDRERTEDKGELKEPFPDGISMLPSQTIDVASSK 540
            LKPVEFD+EPC  D IFYRYVFSDDAL RERTEDKGELKE FP+GI ML  QTIDVAS K
Sbjct: 481  LKPVEFDREPCENDGIFYRYVFSDDALVRERTEDKGELKESFPEGIPMLSGQTIDVASRK 540

Query: 541  LLNDGCQQLGRQNLVAVGNTEKVLSSWKSQSELNDPKFFLNSQKPAGTRKGESVFSATDI 600
            LLN+ CQQ  RQ L AVGN+EK LSSWKS+SE ND KFFLNSQKPAG RK ESVFSA D 
Sbjct: 541  LLNERCQQKERQGLAAVGNSEKALSSWKSKSESNDLKFFLNSQKPAGARKSESVFSAIDT 600

Query: 601  NTILPKVPCDGQLTNNPSTTSADVSLKQLNVTLHQVCLSDNILHLINNSEKTYLAILQNE 660
            NT LPKV  DG LTN PS +SAD SLKQLNV +HQVCLSDNILHLINNSEKTYLAILQNE
Sbjct: 601  NTTLPKVSRDGILTNKPSMSSADDSLKQLNVAVHQVCLSDNILHLINNSEKTYLAILQNE 660

Query: 661  TELRKTYLPYVAEDYVLMLSLPKQKLIDCVKKIYLLGTSNYWEEKVMTLATLYAIKQIAW 720
            TELRK YLPYVAEDY+LMLSLPKQKLIDC+KKIYL GT+ YWEEK+MTLATLYA KQI W
Sbjct: 661  TELRKKYLPYVAEDYLLMLSLPKQKLIDCIKKIYLQGTNTYWEEKIMTLATLYATKQIVW 720

Query: 721  YLCFYGIHPAHLYLKKLCQSLECLKSRLGFLASLIEDASKMVDREITISHPALTTIQDIL 780
            YLCF+GIHPAHLYLKKLCQS ECLKSRLGFL S IE+A KMVD+EITISHPALTTIQ+IL
Sbjct: 721  YLCFFGIHPAHLYLKKLCQSSECLKSRLGFLVSSIEEAGKMVDKEITISHPALTTIQEIL 780

Query: 781  CSKTSSSTLKVLVVANEIFWGSLKKLFGSMGLSFEELSFGGPTDQQVFNANSMVDELVPN 840
            CS+TS+S+LKVLVVAN+IFW SLK L GS+G+SF E+++G  TD+QV NAN+MVD+L  N
Sbjct: 781  CSRTSTSSLKVLVVANQIFWWSLKILLGSLGISFAEINYGSLTDEQVSNANAMVDDLASN 840

Query: 841  CLLVSQGHISGSFPFNKFSIILEYGGINGSSQISTYFTKSINMPHLHFVMLELEKCGNCK 900
            CLLVSQ +ISGSFPFNKFSI+LEYGG+NGSSQIS YF+  I+MPHLHF+MLEL+KCGNCK
Sbjct: 841  CLLVSQEYISGSFPFNKFSIVLEYGGLNGSSQISAYFSNLIDMPHLHFIMLELDKCGNCK 900

Query: 901  AFCKGVGLPQHNELKIEEKSLVENQTRMLEELLNFLPVDEKNILAPPEATIEAEDLKVPL 960
            AFC+GV  PQHNEL IEEKSLVENQT MLE+LLNFLPV+EK ILA  + TIEAE+ +VPL
Sbjct: 901  AFCEGVDFPQHNELTIEEKSLVENQTMMLEKLLNFLPVEEKYILASSKKTIEAENRRVPL 960

Query: 961  QVSVGPVSGKSQHIDLVSFPEAIVIVNTQKFEKEMIVSRRSSYQRLLALEKDGVQVVERD 1020
            +  V PVS KSQH DLVSFPEAI+IVNTQKFEKEMIV RRSSYQR+LALEK+GVQVVERD
Sbjct: 961  RAPVVPVSDKSQHTDLVSFPEAIIIVNTQKFEKEMIVCRRSSYQRILALEKEGVQVVERD 1020

Query: 1021 LSFPVDLIITSGLCLMWYDCTNIHRKASTPNEASSCMNLCIENIATDVLTSLSFAFSGCI 1080
            L  PVDLII  G+CL WYDCTNI RK ST NEAS C+NLCIENIATDVLTSLSFAF GC+
Sbjct: 1021 LCLPVDLIIAPGICLFWYDCTNIDRKGSTSNEASLCLNLCIENIATDVLTSLSFAFRGCV 1080

Query: 1081 LVFEGEISYLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKSMNRH 1140
            LVFEGEIS LSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSK M R 
Sbjct: 1081 LVFEGEISSLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKLMTRR 1140

Query: 1141 LYPKMSESETLAESFLTSFPSINPLTAHGILSSESFLCDFLEWPHERRLHAVKKYCIPDE 1200
            +YPKMSESETLAESFLTSFPSINPL AHGILSSES L DFLEWPHERRLHA++KY IPD+
Sbjct: 1141 IYPKMSESETLAESFLTSFPSINPLIAHGILSSESLLADFLEWPHERRLHAIRKYSIPDD 1200

Query: 1201 SVSLFSVLCKYGEREDSKSVMTDCSSSVSSGPDSDKCYFNGNSKRKRRNFTGGTQCIDKN 1260
             +SLFS LCKYGEREDSKSVMTDCSSS+SSG DS+KC+FNGNS+RKRRNFTGGTQCI+KN
Sbjct: 1201 CISLFSALCKYGEREDSKSVMTDCSSSLSSGADSEKCHFNGNSERKRRNFTGGTQCIEKN 1260

Query: 1261 MDVFNSDAPNPFSAGSRETLSASKSLGSQMFEDQEIFSGLKGLSSSVNHFFDQNHNLESF 1320
             D   S+  NPF+AG+ ETL ASKS GSQ+FED EIF  LKGLSSSVN+FFDQNHNL SF
Sbjct: 1261 TDCLYSNTLNPFTAGTAETLVASKSFGSQLFEDPEIFCDLKGLSSSVNNFFDQNHNLASF 1320

Query: 1321 DASISMNPNSFGKSRDSWISTSPEISNEIRRHCSSYVQNQGLDSNRRKRHNLHNMNKSEN 1380
            DA++SM+P    K RD WIST+PEIS+EIRR CSS+VQNQGLD +++K HN HNMNKSEN
Sbjct: 1321 DATVSMDPTRVCKPRDCWISTAPEISDEIRRRCSSFVQNQGLDRSKKKIHNFHNMNKSEN 1380

Query: 1381 QHEELTGEVVSLIDDPVFKDDFASMAPMNFLPSMLDYEKDSPRKSKIQRKLYHGQSDHPF 1440
             HEEL  EV +LID+PV KD FA+MAPMNFLPSMLD E DS RKSKIQR+L +GQSDHPF
Sbjct: 1381 HHEELIAEVDNLIDNPVLKDHFATMAPMNFLPSMLDNETDSSRKSKIQRRLSYGQSDHPF 1440

Query: 1441 SAVGMANNSSSDFWSPMNFHRQNSGGLDNHFPDPSFQP-----RYKDDHSDEGLMQNSVR 1500
             AV + NNSSSDFWS +N H Q+S GLDN FPDPSF+P     RYKDDH DEGL+QN +R
Sbjct: 1441 CAVDVGNNSSSDFWSSINLHGQSSRGLDNQFPDPSFEPSIMPLRYKDDHLDEGLIQNPLR 1500

Query: 1501 DSKVLFSLSEKGMSHSDVTPLSIALRSKHLQESSPWTMEFLNRIKEKSRNRLHSVPCGSS 1560
            DSKV FSLSEK  SHSDVTPLS+ALRSKHLQESSPWTMEFLNRI+EKSRNR HSVP GSS
Sbjct: 1501 DSKVSFSLSEKDTSHSDVTPLSVALRSKHLQESSPWTMEFLNRIREKSRNRQHSVPRGSS 1560

Query: 1561 SPFPEHLGNVRKTVKRRSPSILEFFKYQGGSTQRKKPEQKRQKQSMQSSNSSKNVLVPTN 1620
            SPFPE+LGNV+KTVKRRSPSILEFFKYQGGST RKKPEQKR K S QSSNSSKNVLV T 
Sbjct: 1561 SPFPENLGNVKKTVKRRSPSILEFFKYQGGSTLRKKPEQKRHKPSTQSSNSSKNVLVATR 1620

Query: 1621 ELSSWTPIDKRSRQTLSFAIDGNERQTKLVWSNDNYGLGQSSQKLTNK 1648
            ELSS TPIDKRSRQTLS+A DGN  QTKLVWSNDNYGLG++SQKL NK
Sbjct: 1621 ELSSGTPIDKRSRQTLSYATDGNGSQTKLVWSNDNYGLGKNSQKLGNK 1667

BLAST of Sed0022815 vs. NCBI nr
Match: XP_023554171.1 (protein SHORTAGE IN CHIASMATA 1 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2521.9 bits (6535), Expect = 0.0e+00
Identity = 1305/1668 (78.24%), Postives = 1429/1668 (85.67%), Query Frame = 0

Query: 1    MRTRFLDIDYFAAGNEPFRCLPVPHLASKPPLSTAGDLLHFDFLPEISLAIDRLAIDSAL 60
            MRTRFL+ID+FA GNE F  LPVPHL S  PLST GDLLHFDFLPEISL ID L+IDSA+
Sbjct: 1    MRTRFLNIDFFAPGNESFHRLPVPHLVSN-PLSTVGDLLHFDFLPEISLGIDSLSIDSAI 60

Query: 61   SKFFDDVLPRRIDDDNDYRD-----SRFQGSTERI--------RFLEEEAKCAYEDNVEG 120
            S+FFDDVLPRRI+DD+ YRD     S F GS ERI        R  E+EAK  YEDN+EG
Sbjct: 61   SRFFDDVLPRRIEDDDVYRDAGCQSSGFHGSAERIFSSGSVGTRLREQEAKGTYEDNMEG 120

Query: 121  GWKNFGSETSETEF--KAIGTVIRNRNLTYDMIQFETPQLDAFLENAFLFEREEVQILAE 180
             WKNFGSETSE EF  K IGT IRNRN + DMIQFETPQLDAFLENAFLFE+EEVQIL  
Sbjct: 121  SWKNFGSETSEMEFIKKDIGTEIRNRNFSNDMIQFETPQLDAFLENAFLFEKEEVQILTG 180

Query: 181  FPEAKFNLETLNQGLLNCPSEVKESVYAIEGITSEYLLDQKSCLVEEDFAQDQKLLHRLT 240
             PE +F+LETLNQGLL  PSEVKES+Y +E I SEYLLDQ+SCL E+DFAQDQKL H+ T
Sbjct: 181  MPEVEFDLETLNQGLLKYPSEVKESIYDVEIIPSEYLLDQESCLFEDDFAQDQKLSHQFT 240

Query: 241  FPFLEVDEMVLETSAFFSMQDELCFVLDNSESPHSIQDDNLLVNDEENLCSMKSDIEEFL 300
            FPFLEVDEMVLET AF S+QDEL F+L+N+ES H IQDDNLLVN+EE L SM+ D EEFL
Sbjct: 241  FPFLEVDEMVLETLAFLSLQDELFFILENTESAHMIQDDNLLVNNEEYLSSMRCDAEEFL 300

Query: 301  LDHFLRPCAVSEVASPDISGG-SDLMSLMEILEIPVSSAVQTKPSCDLPVGLAIFEEFQL 360
             DHFLRPCAVSE+ASPDISGG SDLMS+ME LEI VSS VQTK SCDL V  AIFEEFQL
Sbjct: 301  SDHFLRPCAVSELASPDISGGLSDLMSMMETLEIAVSSEVQTKSSCDLSVEPAIFEEFQL 360

Query: 361  LDIYSNQPFEAFFDLELSAVSEISDCMSIENMNFKSFNELIVCHELALVDDTFKSLPVPL 420
            LD YSNQ F    DLE+SA+SEI+DC SIEN NFKSFNELIVCHEL LVDDTFKSLPVP+
Sbjct: 361  LDTYSNQHFGVILDLEVSAMSEITDCTSIENTNFKSFNELIVCHELVLVDDTFKSLPVPI 420

Query: 421  LSSEESERPMFAFIEDVLANLNGKSLSTSDGIYLDWYLLEEDSCSFGIYSSYQNLLEEIN 480
            LSS+ SE+P+ AFIEDVLANLN +SLS SDGIYLDW+LL+E S S G+YSSYQN+LEEIN
Sbjct: 421  LSSQGSEKPLNAFIEDVLANLNKQSLSASDGIYLDWHLLDESSYSSGLYSSYQNMLEEIN 480

Query: 481  LKPVEFDQEPCGYDSIFYRYVFSDDALDRERTEDKGELKEPFPDGISMLPSQTIDVASSK 540
            LKPVEFDQEPC  D IFYRYVFSDDAL RERTEDKGELKE FP+GI ML  QTIDVASSK
Sbjct: 481  LKPVEFDQEPCENDGIFYRYVFSDDALVRERTEDKGELKESFPEGIPMLSGQTIDVASSK 540

Query: 541  LLNDGCQQLGRQNLVAVGNTEKVLSSWKSQSELNDPKFFLNSQKPAGTRKGESVFSATDI 600
            LLN+ CQQ  RQ L AVGN+EK LSSWKS+SE ND KFFLNSQKPA  RK ESVFS  D 
Sbjct: 541  LLNERCQQKERQGLAAVGNSEKALSSWKSKSESNDLKFFLNSQKPARVRKSESVFSEIDT 600

Query: 601  NTILPKVPCDGQLTNNPSTTSADVSLKQLNVTLHQVCLSDNILHLINNSEKTYLAILQNE 660
            NT LPKVP DG LTN PS +SAD SLKQLNV +HQVCLSDNILHLINNSEKTYLAILQNE
Sbjct: 601  NTTLPKVPRDGILTNKPSMSSADDSLKQLNVAVHQVCLSDNILHLINNSEKTYLAILQNE 660

Query: 661  TELRKTYLPYVAEDYVLMLSLPKQKLIDCVKKIYLLGTSNYWEEKVMTLATLYAIKQIAW 720
            TELRK YLPYVAEDY+LMLSLPKQ LIDC+KKIYL GT+ YWEEK+MTLATLYA KQI W
Sbjct: 661  TELRKKYLPYVAEDYLLMLSLPKQMLIDCIKKIYLQGTNTYWEEKIMTLATLYATKQIVW 720

Query: 721  YLCFYGIHPAHLYLKKLCQSLECLKSRLGFLASLIEDASKMVDREITISHPALTTIQDIL 780
            YLCFYGIHPAHLYLKKLCQS ECLKSRLGFL S IE+A KMV REITISHPALTTIQ+IL
Sbjct: 721  YLCFYGIHPAHLYLKKLCQSSECLKSRLGFLVSSIEEAGKMVGREITISHPALTTIQEIL 780

Query: 781  CSKTSSSTLKVLVVANEIFWGSLKKLFGSMGLSFEELSFGGPTDQQVFNANSMVDELVPN 840
            CS+TS S+LKVLVVAN+IFW SLKKL GS+G+SF EL++G  TD+QV NAN+MVD+L  N
Sbjct: 781  CSRTSISSLKVLVVANQIFWWSLKKLLGSLGISFAELNYGSLTDEQVSNANAMVDDLASN 840

Query: 841  CLLVSQGHISGSFPFNKFSIILEYGGINGSSQISTYFTKSINMPHLHFVMLELEKCGNCK 900
            CLLVS+ +ISGSFPFNKFSI+LEYGG+NGSSQIS YF+  I+MPHLHF+MLEL+KCGNCK
Sbjct: 841  CLLVSEEYISGSFPFNKFSIVLEYGGLNGSSQISAYFSNLIDMPHLHFIMLELDKCGNCK 900

Query: 901  AFCKGVGLPQHNELKIEEKSLVENQTRMLEELLNFLPVDEKNILAPPEATIEAEDLKVPL 960
            AFC+GV  PQHNEL IEEKSLVENQT MLE+LLNFLPV+EK ILA P+ TIEAE+ +V L
Sbjct: 901  AFCEGVDFPQHNELTIEEKSLVENQTMMLEKLLNFLPVEEKYILASPKKTIEAENRRVSL 960

Query: 961  QVSVGPVSGKSQHIDLVSFPEAIVIVNTQKFEKEMIVSRRSSYQRLLALEKDGVQVVERD 1020
            +  V PVS KSQH DLVSFPEAI+IVNTQKFEKEMIV RRSSYQR+LALEK+GVQVVERD
Sbjct: 961  RAPVVPVSDKSQHTDLVSFPEAIIIVNTQKFEKEMIVCRRSSYQRILALEKEGVQVVERD 1020

Query: 1021 LSFPVDLIITSGLCLMWYDCTNIHRKASTPNEASSCMNLCIENIATDVLTSLSFAFSGCI 1080
            L  PVDLII  G+CL WYDCTNI RK ST NEAS  +NLCIENIATDVLTSLSFAF GC+
Sbjct: 1021 LCLPVDLIIAPGICLFWYDCTNIDRKGSTSNEASLSLNLCIENIATDVLTSLSFAFRGCV 1080

Query: 1081 LVFEGEISYLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKSMNRH 1140
            LVFEGEIS LSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSK M R 
Sbjct: 1081 LVFEGEISSLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKLMTRR 1140

Query: 1141 LYPKMSESETLAESFLTSFPSINPLTAHGILSSESFLCDFLEWPHERRLHAVKKYCIPDE 1200
            +YPKMSESETLAESFLTSFPSINPL AHGILSSES L DFLEWPHERRLHA++KY IPD+
Sbjct: 1141 IYPKMSESETLAESFLTSFPSINPLIAHGILSSESLLADFLEWPHERRLHAIRKYSIPDD 1200

Query: 1201 SVSLFSVLCKYGEREDSKSVMTDCSSSVSSGPDSDKCYFNGNSKRKRRNFTGGTQCIDKN 1260
             +SLFS LCKYGEREDSKSVMTDCSSS+SSG DS+KC+FNGNS+RKRRNFTGG+QCI+KN
Sbjct: 1201 CISLFSALCKYGEREDSKSVMTDCSSSLSSGADSEKCHFNGNSERKRRNFTGGSQCIEKN 1260

Query: 1261 MDVFNSDAPNPFSAGSRETLSASKSLGSQMFEDQEIFSGLKGLSSSVNHFFDQNHNLESF 1320
             D   S+  NPF+AG+ ETL ASKS GSQ+FED EIF  LKGLSSSVN+FFDQNHNL SF
Sbjct: 1261 TDCLYSNTLNPFTAGTAETLVASKSFGSQLFEDPEIFCDLKGLSSSVNNFFDQNHNLASF 1320

Query: 1321 DASISMNPNSFGKSRDSWISTSPEISNEIRRHCSSYVQNQGLDSNRRKRHNLHNMNKSEN 1380
            DA++SM+P    K RD WIST+PEIS+EIRRHCSS+VQNQGLD +++K HN HNMNKSEN
Sbjct: 1321 DATVSMDPTRVCKPRDCWISTAPEISDEIRRHCSSFVQNQGLDRSKKKIHNFHNMNKSEN 1380

Query: 1381 QHEELTGEVVSLIDDPVFKDDFASMAPMNFLPSMLDYEKDSPRKSKIQRKLYHGQSDHPF 1440
             HEEL  EV +LID+PV KD FA+MAPMNFLPSML+ E DS RKSKIQR+L +GQSDHPF
Sbjct: 1381 HHEELIAEVDNLIDNPVLKDHFATMAPMNFLPSMLENETDSSRKSKIQRRLSYGQSDHPF 1440

Query: 1441 SAVGMANNSSSDFWSPMNFHRQNSGGLDNHFPDPSFQP-----RYKDDHSDEGLMQNSVR 1500
             AV + NNSSSDFWS +N H Q+S GLDN FPDPSF+P     RYKDDH DEGL QN +R
Sbjct: 1441 CAVDVGNNSSSDFWSSINLHGQSSRGLDNQFPDPSFEPSIMPLRYKDDHLDEGLTQNPLR 1500

Query: 1501 DSKVLFSLSEKGMSHSDVTPLSIALRSKHLQESSPWTMEFLNRIKEKSRNRLHSVPCGSS 1560
            DSKV FSLSEK  SHSDVTPLS+ALRSKHLQESSPWTMEFLNRI+EKSRNR HSVP GSS
Sbjct: 1501 DSKVSFSLSEKHTSHSDVTPLSVALRSKHLQESSPWTMEFLNRIREKSRNRQHSVPRGSS 1560

Query: 1561 SPFPEHLGNVRKTVKRRSPSILEFFKYQGGSTQRKKPEQKRQKQSMQSSNSSKNVLVPTN 1620
            SPFPE+LGNV+KTVKRRSPSILEFFKYQGGST RKKPEQKR K S QSSNSSKNVLV T 
Sbjct: 1561 SPFPENLGNVKKTVKRRSPSILEFFKYQGGSTLRKKPEQKRHKPSTQSSNSSKNVLVATR 1620

Query: 1621 ELSSWTPIDKRSRQTLSFAIDGNERQTKLVWSNDNYGLGQSSQKLTNK 1648
            ELSS TPIDKRSRQTLS+A DGN  QTKLVWSNDNYGLG++SQKL NK
Sbjct: 1621 ELSSGTPIDKRSRQTLSYATDGNGSQTKLVWSNDNYGLGKNSQKLGNK 1667

BLAST of Sed0022815 vs. NCBI nr
Match: XP_022952237.1 (protein SHORTAGE IN CHIASMATA 1 isoform X3 [Cucurbita moschata])

HSP 1 Score: 2516.9 bits (6522), Expect = 0.0e+00
Identity = 1299/1666 (77.97%), Postives = 1427/1666 (85.65%), Query Frame = 0

Query: 1    MRTRFLDIDYFAAGNEPFRCLPVPHLASKPPLSTAGDLLHFDFLPEISLAIDRLAIDSAL 60
            MRTRFL+ID+FA GNE F  LPVPHL S  PLST GDLLHFDFLPEISL ID L+IDSA+
Sbjct: 1    MRTRFLNIDFFAPGNESFHRLPVPHLVSN-PLSTVGDLLHFDFLPEISLGIDSLSIDSAI 60

Query: 61   SKFFDDVLPRRIDDDNDYRD-----SRFQGSTERI--------RFLEEEAKCAYEDNVEG 120
            S+FFDDVLPRRI DD+ YRD     S F GS ERI        R  EEEAK  YEDN+EG
Sbjct: 61   SRFFDDVLPRRIVDDDVYRDAGCQSSGFHGSAERIFSSESVGTRLREEEAKGTYEDNMEG 120

Query: 121  GWKNFGSETSETEFKAIGTVIRNRNLTYDMIQFETPQLDAFLENAFLFEREEVQILAEFP 180
             WKNFGSETSE EFK IGT IRNRN + DMIQFETPQLD FLENAFLFE+EEVQIL   P
Sbjct: 121  SWKNFGSETSEMEFKDIGTEIRNRNFSNDMIQFETPQLDTFLENAFLFEKEEVQILTAMP 180

Query: 181  EAKFNLETLNQGLLNCPSEVKESVYAIEGITSEYLLDQKSCLVEEDFAQDQKLLHRLTFP 240
            E +F+LETLNQGLL  PSEVKES+Y +E I SEYLLDQ+SCL E+DFAQDQKL H+ TFP
Sbjct: 181  EVEFDLETLNQGLLKYPSEVKESIYDVEIIPSEYLLDQESCLFEDDFAQDQKLSHQFTFP 240

Query: 241  FLEVDEMVLETSAFFSMQDELCFVLDNSESPHSIQDDNLLVNDEENLCSMKSDIEEFLLD 300
            FLEVDEMVLET AF S+QDEL F+L+++ES H I DDNLLVN+EE L SM+ D EEFL D
Sbjct: 241  FLEVDEMVLETLAFLSLQDELFFILESTESAHMIHDDNLLVNNEEYLSSMRCDAEEFLSD 300

Query: 301  HFLRPCAVSEVASPDISGG-SDLMSLMEILEIPVSSAVQTKPSCDLPVGLAIFEEFQLLD 360
            HFLRPC VSE+ASPDISGG SDLMS+ME LEI VSS VQTK SCDL V  AIFEEFQLLD
Sbjct: 301  HFLRPCGVSELASPDISGGSSDLMSMMETLEIAVSSEVQTKSSCDLSVEPAIFEEFQLLD 360

Query: 361  IYSNQPFEAFFDLELSAVSEISDCMSIENMNFKSFNELIVCHELALVDDTFKSLPVPLLS 420
             YSNQ F A FDLE+SA+SEI+DC SIEN NFKSFNELIVCHEL LVDDTFKSLPVP+LS
Sbjct: 361  TYSNQHFGAIFDLEVSAMSEITDCTSIENTNFKSFNELIVCHELVLVDDTFKSLPVPILS 420

Query: 421  SEESERPMFAFIEDVLANLNGKSLSTSDGIYLDWYLLEEDSCSFGIYSSYQNLLEEINLK 480
            S+ SE+ + AFIEDVLANLN +SLS SDGIYLDW+LL+E S S G+YSSYQN+LEEINLK
Sbjct: 421  SQGSEKLLNAFIEDVLANLNKQSLSASDGIYLDWHLLDESSYSSGLYSSYQNMLEEINLK 480

Query: 481  PVEFDQEPCGYDSIFYRYVFSDDALDRERTEDKGELKEPFPDGISMLPSQTIDVASSKLL 540
            PVEFD+EPC  D IFYRYVFSDDAL RERTEDKGELKE FP+GI ML  QTIDVASS LL
Sbjct: 481  PVEFDREPCENDGIFYRYVFSDDALVRERTEDKGELKESFPEGIPMLSGQTIDVASSILL 540

Query: 541  NDGCQQLGRQNLVAVGNTEKVLSSWKSQSELNDPKFFLNSQKPAGTRKGESVFSATDINT 600
            N+ CQQ  RQ L AVGN+EK LSSWKS+SE ND KFFLNSQKP G RK ESVFS  D NT
Sbjct: 541  NERCQQKERQGLAAVGNSEKALSSWKSKSESNDLKFFLNSQKPVGVRKSESVFSEIDTNT 600

Query: 601  ILPKVPCDGQLTNNPSTTSADVSLKQLNVTLHQVCLSDNILHLINNSEKTYLAILQNETE 660
             LPKVP DG LTN PS +SAD SLKQLNV +HQVCLSDNILHLINNSEKTYLAILQNETE
Sbjct: 601  TLPKVPRDGILTNKPSMSSADDSLKQLNVAVHQVCLSDNILHLINNSEKTYLAILQNETE 660

Query: 661  LRKTYLPYVAEDYVLMLSLPKQKLIDCVKKIYLLGTSNYWEEKVMTLATLYAIKQIAWYL 720
            LRK YLPYVAEDY+LMLSLPKQKLIDC+KKIYL GT+ YWEEK+MTLATLYA KQI WYL
Sbjct: 661  LRKKYLPYVAEDYLLMLSLPKQKLIDCIKKIYLQGTNTYWEEKIMTLATLYATKQIVWYL 720

Query: 721  CFYGIHPAHLYLKKLCQSLECLKSRLGFLASLIEDASKMVDREITISHPALTTIQDILCS 780
            CFYGIHPAHLYLKKLCQS ECLKSRLGFL S IE+A KMV REITISHPALTTIQ+ILCS
Sbjct: 721  CFYGIHPAHLYLKKLCQSSECLKSRLGFLVSSIEEAGKMVGREITISHPALTTIQEILCS 780

Query: 781  KTSSSTLKVLVVANEIFWGSLKKLFGSMGLSFEELSFGGPTDQQVFNANSMVDELVPNCL 840
            +TS+S+LKVLVVAN+IFW SL+KL GS+G+SF E+++G  T +QV NAN+MVD+L  NCL
Sbjct: 781  RTSTSSLKVLVVANQIFWWSLEKLLGSLGISFAEINYGSLTVEQVSNANAMVDDLASNCL 840

Query: 841  LVSQGHISGSFPFNKFSIILEYGGINGSSQISTYFTKSINMPHLHFVMLELEKCGNCKAF 900
            LVSQ +ISGSFPFNKFSI+LEYGG+NGSSQIS YF+  I+MPHLHF+MLEL+KCGNCKAF
Sbjct: 841  LVSQEYISGSFPFNKFSIVLEYGGLNGSSQISAYFSNLIDMPHLHFIMLELDKCGNCKAF 900

Query: 901  CKGVGLPQHNELKIEEKSLVENQTRMLEELLNFLPVDEKNILAPPEATIEAEDLKVPLQV 960
            C+GV  PQHNEL IEEKSLVENQT MLE+LLNFLPV+EK ILA P+ TIEAE+ +VPL+ 
Sbjct: 901  CEGVDFPQHNELTIEEKSLVENQTMMLEKLLNFLPVEEKYILASPKKTIEAENRRVPLRA 960

Query: 961  SVGPVSGKSQHIDLVSFPEAIVIVNTQKFEKEMIVSRRSSYQRLLALEKDGVQVVERDLS 1020
             V PVS KSQ+ DLVSFPEAI+IVNTQKFEKEMIV RRSSY+R+LALEK+GVQVVERDL 
Sbjct: 961  PVEPVSDKSQYTDLVSFPEAIIIVNTQKFEKEMIVCRRSSYRRILALEKEGVQVVERDLC 1020

Query: 1021 FPVDLIITSGLCLMWYDCTNIHRKASTPNEASSCMNLCIENIATDVLTSLSFAFSGCILV 1080
             PVDLII  G+CL WYDCTNI RK ST NEAS C+NLCIENIATDVLTSLSFAF GC+LV
Sbjct: 1021 LPVDLIIAPGICLFWYDCTNIDRKGSTSNEASLCLNLCIENIATDVLTSLSFAFRGCVLV 1080

Query: 1081 FEGEISYLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKSMNRHLY 1140
            FEGEIS LSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSK M R +Y
Sbjct: 1081 FEGEISSLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKLMTRRIY 1140

Query: 1141 PKMSESETLAESFLTSFPSINPLTAHGILSSESFLCDFLEWPHERRLHAVKKYCIPDESV 1200
            PKMSESETLAESFLTSFPSINPL AHGILSSES L DFLEWPHERRLHA++KY IPD+ +
Sbjct: 1141 PKMSESETLAESFLTSFPSINPLIAHGILSSESLLADFLEWPHERRLHAIRKYSIPDDCI 1200

Query: 1201 SLFSVLCKYGEREDSKSVMTDCSSSVSSGPDSDKCYFNGNSKRKRRNFTGGTQCIDKNMD 1260
            SLFS LCKYGEREDSKSVMTDCSSS+SSG DS+KC+FNGNS+RKRRNFTGGTQCI+KN D
Sbjct: 1201 SLFSALCKYGEREDSKSVMTDCSSSLSSGADSEKCHFNGNSERKRRNFTGGTQCIEKNTD 1260

Query: 1261 VFNSDAPNPFSAGSRETLSASKSLGSQMFEDQEIFSGLKGLSSSVNHFFDQNHNLESFDA 1320
               S+  NPF+AG+ ETL ASKS GSQ+FED EIF  LKGLSSSVN+FFDQNHNL SFDA
Sbjct: 1261 CLYSNTLNPFTAGTAETLVASKSFGSQLFEDPEIFCDLKGLSSSVNNFFDQNHNLASFDA 1320

Query: 1321 SISMNPNSFGKSRDSWISTSPEISNEIRRHCSSYVQNQGLDSNRRKRHNLHNMNKSENQH 1380
            ++SM+P    K RD WIST+PEIS+EIRRHCS +VQNQGLD +++K H+ HNMNKSEN H
Sbjct: 1321 TVSMDPTRVCKPRDCWISTAPEISDEIRRHCSPFVQNQGLDRSKKKIHSFHNMNKSENHH 1380

Query: 1381 EELTGEVVSLIDDPVFKDDFASMAPMNFLPSMLDYEKDSPRKSKIQRKLYHGQSDHPFSA 1440
            EEL  EV +LID+PV KD FA+MAPMNFLPSML+ E DS RKSKIQR+L +GQSDHPF A
Sbjct: 1381 EELIAEVDNLIDNPVLKDHFATMAPMNFLPSMLENETDSSRKSKIQRRLSYGQSDHPFCA 1440

Query: 1441 VGMANNSSSDFWSPMNFHRQNSGGLDNHFPDPSFQP-----RYKDDHSDEGLMQNSVRDS 1500
            V + NNSSSDFWS +N H Q+S GLDN FPDPSF+P     RYKDDH DEGL QN +RDS
Sbjct: 1441 VDVGNNSSSDFWSSINLHGQSSRGLDNQFPDPSFEPSIMPLRYKDDHLDEGLTQNHLRDS 1500

Query: 1501 KVLFSLSEKGMSHSDVTPLSIALRSKHLQESSPWTMEFLNRIKEKSRNRLHSVPCGSSSP 1560
            KV FSLSEK  SHSDVTPLS+ALRSKHLQESSPWTMEFLNRI+EKSRNR HSVP GSSSP
Sbjct: 1501 KVSFSLSEKDTSHSDVTPLSVALRSKHLQESSPWTMEFLNRIREKSRNRQHSVPRGSSSP 1560

Query: 1561 FPEHLGNVRKTVKRRSPSILEFFKYQGGSTQRKKPEQKRQKQSMQSSNSSKNVLVPTNEL 1620
            FPE+LGNV+KTVKRRSPSILEFFKYQGGST RKKPEQKR K S QSSNSSKNVLV T EL
Sbjct: 1561 FPENLGNVKKTVKRRSPSILEFFKYQGGSTLRKKPEQKRHKPSTQSSNSSKNVLVATREL 1620

Query: 1621 SSWTPIDKRSRQTLSFAIDGNERQTKLVWSNDNYGLGQSSQKLTNK 1648
            SS TPIDKRSRQTLS+A DGN  QTKLVWSNDNYGLG++SQKL NK
Sbjct: 1621 SSGTPIDKRSRQTLSYATDGNGSQTKLVWSNDNYGLGKNSQKLGNK 1665

BLAST of Sed0022815 vs. ExPASy Swiss-Prot
Match: F4KG50 (Protein SHORTAGE IN CHIASMATA 1 OS=Arabidopsis thaliana OX=3702 GN=SHOC1 PE=1 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 2.8e-225
Identity = 608/1682 (36.15%), Postives = 900/1682 (53.51%), Query Frame = 0

Query: 1    MRTRFLDIDYFAAGNE------PFRCLPVPHLASKPPL-STAGDLLHFDFLPEISLAIDR 60
            MRTRFL+IDYF+           F  LP P     P + +   D L F  +  +S+ I  
Sbjct: 1    MRTRFLNIDYFSTPPSHVFETLGFLNLPAPDNFPAPIVYNGEEDRLRFGSIENVSIPIGN 60

Query: 61   LAIDSALSKFFDDVLPRRIDDDNDYRDSRFQGSTERIRFLEEEAKCAYEDNVEGGWKNFG 120
            L I++ALSKF  DV+P R+    DYR      S+  + + +E                  
Sbjct: 61   LPIEAALSKFLSDVVPDRV--SVDYRVFEIDDSSLGVYYSDE------------------ 120

Query: 121  SETSETEFKAIGTVIRNRNLTYDMIQFETPQLDAFLENAFLFEREE-VQILAEFPEAKFN 180
                    K  G  I ++  T  +I+ ETP+LD  +EN  L   E+ +Q  +E  E K N
Sbjct: 121  --------KDDGDAIADK-ATPKIIELETPELDFEMENKLLCTSEDHLQCFSEVLEIK-N 180

Query: 181  LETLNQG---LLNCPSEVKESVYAIEGITSEYLLDQKSCLVE-EDFAQDQKLLHRLTFPF 240
                 +G   +L    +++E +Y+++ I S+Y  +  + + E E F + Q       FP 
Sbjct: 181  DPVKYEGSDIILQNSKDIQEQIYSVDYIPSDYFTENNTSVAENECFRKIQPWFKDARFPL 240

Query: 241  LEVDEMVLETSAFFSMQDELCFVLDNSESPHSIQDDNLLVNDEENLCSMKSDIEEFL-LD 300
            LEVDE+ L   +  S+ D++  VL+  E   +    +L++N +E + S   D+ + L  D
Sbjct: 241  LEVDEVNLSELSSLSVLDKVFTVLETIEPQDTNAGSSLIINSKELIGSKDYDLLDVLSTD 300

Query: 301  HFLRPCAVSEVASPDISGGSDLMSLMEILEIPVSSAVQTKPSCDLPVGLAIFEEFQLLDI 360
             +L     S+V   D     D+++++EI     S+A + +    +PV    +EEFQ+LD+
Sbjct: 301  CYLNKSGQSDVVPEDEFSEMDIVTILEI-----SNAEEFQGKVAVPV---TYEEFQILDV 360

Query: 361  YSNQPFEAFFDLELSAVSEISDCMSIENMNFKSFNELIVCHELALVDDTFKSLPVPLLSS 420
              +  F+ F  L+ +   EI   M  + MNFK F+EL+V  ELA  DD FKSLP P+L  
Sbjct: 361  DISDVFDIFLCLQKAIEPEICYGMFSKEMNFKDFDELVVSSELAFTDDAFKSLPTPILHD 420

Query: 421  EESERPMFAFIEDVLANLNGKSLSTSDGIYLDWYLLEEDSCSFGIYSSYQNLLEEINLKP 480
             E  R +    EDVL+ +  +SLS S+ IYL W LLEE + +   Y       EEI    
Sbjct: 421  YEMTRSLELIYEDVLSKIKPQSLSASNDIYLPWNLLEERNHNHCDYP-----FEEIVTFN 480

Query: 481  VEFDQEPCGYDSIFYRYVFSDDALDRERTEDKGELKEPFPDGISMLPSQTIDVASSKLLN 540
            ++++ E    D   Y ++FS+DA      E   +  EPF  GIS L        S  LL 
Sbjct: 481  IDYNWEASEGDKWVYDFIFSEDAFCEPLVE---KCTEPF-YGISNLDEHAPVNTSHGLLE 540

Query: 541  DGCQQLGRQNLVAVGNTEKVLSSWKSQSELNDPKFFLNSQKPAGTRKGES-VFSATDIN- 600
            +  Q+ G ++     N +K    +KS S  +D  FF++ +K       ES V +A   N 
Sbjct: 541  NPFQKTGARDCAVDDNAKKATLLFKSMSAFDDLTFFMDPKKAVIEDNLESRVEAAKTTNH 600

Query: 601  ---TILPKVPC-DGQLTNNPSTTSADVSLKQLNVTLHQVCLSDNILHLINNSEKTYLAIL 660
               +I  K  C  G +  NP T           + LH V  S+NI  L+    K+YL ++
Sbjct: 601  KCMSIDSKASCRSGGMHPNPKTE---------EMILHSVRPSENIQALVGEFVKSYLTLV 660

Query: 661  QNETELRKTYLPYVAEDYVLMLSLPKQKLIDCVKKIYLLGTSNYWEEKVMTLATLYAIKQ 720
            ++E+E        ++ED + +LS+ K KLIDC++K  +  T    ++K  T A L AIKQ
Sbjct: 661  KDESE-------NLSEDKLKLLSISKGKLIDCIRKANVHKT-QLADDKTFTFALLLAIKQ 720

Query: 721  IAWYLCFYGIHPAHLYLKKLCQSLECLKSRLGFLASLIEDASKMVDREITISHPALTTIQ 780
            + WY+CF+GIH A++YL K+C+S   +K  L  L S +E   K  + +IT SHP+L  IQ
Sbjct: 721  MTWYMCFFGIHVAYIYLNKVCRSSNPMKIGLHTLYSAVETEHKSDETDITRSHPSLAVIQ 780

Query: 781  DILCSKTSSSTLKVLVVANEIFWGSLKKLFGSMGLSFEELSFGGPTDQQ--VFNANSMVD 840
             IL S+ +    K L++A ++FW SLK+L  SMGLS+ +L+   P+  +  V  A  +  
Sbjct: 781  GILQSEFARGNSKALLLAEKVFWSSLKRLLMSMGLSYNDLNSPSPSGNRPNVHEAIELGF 840

Query: 841  ELVPNCLLVSQGHISGSFPFNKFSIILEYGGINGSSQISTYFTKSINMPHLHFVMLELEK 900
              + +CL++S   IS SFP   FS+I+EYGG N S + S + +K  + P  HF+ +EL+ 
Sbjct: 841  LPISDCLIISYEQISPSFPVENFSVIVEYGGPNASPRYS-FPSKLDSFPSFHFIKVELDM 900

Query: 901  CGNCKAFCKGVGLPQHNELKIEEKSLVENQTRMLEELLNFLPVDEKNILAPPEATIEAED 960
               C   C GV +P    LK+ +   VE +T  LEE+LNF+P+++       E T E+E 
Sbjct: 901  PSACGQLCAGVTVPY--SLKMIKGDEVETKTGWLEEVLNFVPLEKVCYAGSSETTNESEF 960

Query: 961  LKVPLQVSVGPVSGKSQHI--DLVSFPEAIVIVNTQKFEKEMIVSRRSSYQRLLALEKDG 1020
            + +P +      S + + I    +S   ++++VNT+  +KEMI+SRRS+YQ++LA+EK+G
Sbjct: 961  ISMPQE------SERKRGIIEQGLSDQRSVIVVNTKTVDKEMIISRRSTYQKVLAMEKEG 1020

Query: 1021 VQVVERDLSFPVDLIITSGLCLMWYDCTNIHRKASTPNEASSCMNLCIENIATDVLTSLS 1080
            VQVVERD   PVDL+++  +CL+WYD   + +K++     SS     I +IAT+VLTSLS
Sbjct: 1021 VQVVERDSDLPVDLMLSPAVCLLWYDSETVSKKSAATIGTSSSSLSWIGDIATNVLTSLS 1080

Query: 1081 FAFSGCILVFEGEISYLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENV 1140
            F+FS CI+VFEGE ++L+ VM+SSD LYAAA SL I  Q+FCS S+ LTDEIIL CI++ 
Sbjct: 1081 FSFSTCIMVFEGEPAFLAAVMDSSDELYAAAGSLGISLQMFCSSSANLTDEIILKCIKSS 1140

Query: 1141 SKSMNRHLYPKMSESETLAESFLTSFPSINPLTAHGILSSESFLCDFLEWPHERRLHAVK 1200
             K    H+  KM ESE+LAESFLT FPS+NPLTA  ILSS   L +F++ PH+ ++   +
Sbjct: 1141 VKLSKLHV--KMPESESLAESFLTKFPSVNPLTAQVILSSSGSLLEFMKLPHKSKVERTQ 1200

Query: 1201 KYCIPDESVSLFSVLCKYGEREDSKSVMTDCSSSVSSGPDSDKCYFNGNSKRKRRNFTGG 1260
            KY +P+ESV LFS +C+YG REDS+SVMTD SSSVSSGPDSD  + + +S  K++ +   
Sbjct: 1201 KYHVPEESVDLFSSVCRYGAREDSRSVMTDSSSSVSSGPDSDTHHVSVHSGSKKKQYIAE 1260

Query: 1261 TQCIDKNMDVFNSDAPNPFSAGSRETLSASKSL-GSQMFEDQEI--------FSGLKGLS 1320
               ID  MD     +P+   A ++   S    L  S   +D EI        FS      
Sbjct: 1261 KDEID--MDDLVHFSPSIEFADTQLKSSGDFQLDDSWSSKDHEIFHFDPVTEFSDAPFKP 1320

Query: 1321 SSVNHFFDQ--NHNLESFDASISMNPNSFGKSRDSWISTSPEISNEIR-------RHCSS 1380
            S ++H  D   + + E FD      P S  K    W    P+ S E            S 
Sbjct: 1321 SGISHPNDSWPSKDPERFDK--KSGPGSSSKD-TFWEKDQPDFSVEDSLPGIPELEDWSF 1380

Query: 1381 YVQNQGLDSNR------RKRHNLHNMNKSENQHEELTGEVVSLIDDPVFKDDFASMAPMN 1440
             V+++ +  NR       +  NLH+   SEN   +  GEV+   D  + +D   S     
Sbjct: 1381 PVKDKFMSQNRGCKFPVMRDFNLHDNRNSENFIADYKGEVIDRADKYLEEDFPPSPGYNR 1440

Query: 1441 FLPSMLDY-EKDSPRKSKIQRKL-YHGQSDHPFSAVGMANNSSSDFWSPMNFHRQNSGGL 1500
            F   + D  E++ PRKSK  RKL + G     F      ++SS  + +  +    N+  L
Sbjct: 1441 FARIVSDVNEEELPRKSKSSRKLSFFGSLQPNFPKAADIDSSSERYATEKDSKYDNNTSL 1500

Query: 1501 DNHFPDPSFQPRYKDDHSDEGLMQNSVRDSKVLFSLSEKGMSHSDVTPLSIALRSKHLQE 1560
              +    ++  + +    +E L + S   +  L    E  +SH   +PLS A+RS +  +
Sbjct: 1501 RGY--ADNYPAKRQRTLLEEVLTRRSAVPTTELPFREE--ISHFGGSPLSNAIRSSNQVQ 1560

Query: 1561 SSPWTMEFLNRIKEKSRNR--LHSVPCGSSSPFPEHLGNVRK-TVKRRSPSILEFFKYQG 1620
            SSPWT++FLNR++E+SR R    S+P  +S P  E  GN++K   KR+SPSILEFFKY+G
Sbjct: 1561 SSPWTVDFLNRVRERSRARKQQQSLPSYASPPSLETPGNIKKANTKRKSPSILEFFKYKG 1593

Query: 1621 GSTQRKKPEQKRQKQSMQSSNSSKNVLVPTNELSSWTPIDKRSRQTLSFAIDGNERQTKL 1631
            G+   K  E+KRQK+S  SS S KN     + L S TPIDKR++Q+LS+  +G   QTKL
Sbjct: 1621 GN---KLQEEKRQKRSKNSSASPKNERF-YSPLKSCTPIDKRAKQSLSYTANGT-GQTKL 1593

BLAST of Sed0022815 vs. ExPASy Swiss-Prot
Match: B9F1C0 (Protein SHORTAGE IN CHIASMATA 1 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=SHOC1 PE=1 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 4.4e-138
Identity = 523/1813 (28.85%), Postives = 818/1813 (45.12%), Query Frame = 0

Query: 1    MRTRFLDIDYFAAGNE------------PFRCLPVPHLASKP---PLSTAGDLLHFDFLP 60
            MRTRFL  DYFA  +              F  LPVP L   P   P ++A      D LP
Sbjct: 1    MRTRFLATDYFAPSSSSAAGKALALEFFSFPSLPVPALPPDPHFLPFTSA------DELP 60

Query: 61   EISLAIDRLA---IDSALSKFFDDVLPRRID-----------DDNDYRDSRFQ---GSTE 120
              ++A D L    I SALS F   V+P+ +            DD  Y    +     S E
Sbjct: 61   AATVADDGLGPLPIASALSDFLAAVIPQALPVPTVPAADEVLDDFLYDRGGYGEDFSSWE 120

Query: 121  RIRFLEEEAKCAYEDNVEGGWKNFGSETSETEFKAIGTVIRN----RNLTYDMIQFETPQ 180
               F   +A   Y   V    K+   E S ++   I +V++     + L ++++  E   
Sbjct: 121  FGAFRIPKASEGY--GVINREKDEKGEGSRSDGLEISSVMKRWEQLKELRFEVV--EVDL 180

Query: 181  LDAFLENAFLFEREE----VQILAEFPEAKFNLETLN---QGLLNCPSEVKESVYAIEGI 240
            L A  E+   F  EE    V +L   P+ K +L+ ++      +   S++ ESVY +E +
Sbjct: 181  LMALQEDIASFGEEESGGGVTLLLRVPDMKIHLDFIDIETDIKIRYQSDLPESVYQVEKV 240

Query: 241  -TSEYLLDQKSCLVEEDFAQDQKLLHRLTFPFLEV-------DEMVLETSAFFSMQDELC 300
               +   +  S L E+   +   L H    P LEV       D+ + ET   + + D + 
Sbjct: 241  PVKDNDGNGHSSLREDCCLEIAALDHGAVIPRLEVSRNSWELDDCLTETDR-YGVFDNVV 300

Query: 301  FVLDNSESPHSIQDDNLLVNDEENLCSMKSDIEEFLLDHFLRPCAVSEVASPDISGGSDL 360
              LD ++  HS+          E L S   D+  F+ +    PC   +V  P     +  
Sbjct: 301  RHLDEAQIQHSV------FKSTEFLRSTDMDMLTFVCED--APCHDIQVDKPAEIKAAVE 360

Query: 361  MSLM----EILEIPVSSAVQTKPS---CDLPVGLAIFEEFQLLDIYSNQPFEAFFDLELS 420
            M ++     IL    S+    KP     DLP  + + EE Q++D  S+  F+     E +
Sbjct: 361  MDVVRINGNILLEKNSALYPLKPDGTCSDLPCSI-LLEEVQIIDFPSDNVFKMLVQSETN 420

Query: 421  AVSEISDCMSIENMNFKSFNELIVCHELALVDDTFKSLPVPLLSSEESERPMFAFIEDVL 480
             ++   +    +    +   E +V  ELALVDDTF+SLP P+L+ + + R     I+++L
Sbjct: 421  KMNISDEIFKDDFDPARRLYESMVSCELALVDDTFRSLPTPILNDDIAVRSRVPPIQEIL 480

Query: 481  ANLNGKSLSTSDGIYLDWYLLEEDSCSFGIYSSYQNLLEEINLKPVEFD-QEPCGYDSIF 540
             +L    LS SDGIYLDW+LL E  C+  I  SY +++EE     +  + Q  C   S+F
Sbjct: 481  CSLKPHPLSASDGIYLDWHLLLEGPCNREICCSYASMVEEAKTCHLSSELQRSCQSTSVF 540

Query: 541  YRYVFSDDALDRERTEDKGELKEPF-PDGISMLPSQTIDVASSKLLNDGCQQLG---RQN 600
                F +D     + +D+ +  + + P  +S  P +    A+ K     C+Q G     +
Sbjct: 541  VS-DFLEDFQRSPKLQDEDKHSDIYVPAPLSHDPQKL--EATQK-----CEQEGGTRNHS 600

Query: 601  LVAVGNTEKVLSSWKSQSELNDPKFFLNSQKPAGTRKGESVFSATDINTILPKVPCDGQL 660
             +   + EK  S  +  S   D  F+LN +  + T+ G       + NT    VP   + 
Sbjct: 601  SMKRPSPEKSSSFPELISHSGDLNFYLNVR--SATKSG-----TNNENTSTLDVPHSEEQ 660

Query: 661  TNNPSTTSADVSLKQLNVTLHQVCLSDNILHLINNSEKTYLAILQNETELRKTYLPYVAE 720
              + ST +    L    + +H V  S+ I  LI     +Y + LQ  T  R ++      
Sbjct: 661  ALSLSTRAKVDKL----IEIHPVSPSNLIQGLIEQIHASYTSALQESTYWRHSFSDEQG- 720

Query: 721  DYVLMLSLPKQKLIDCVKKIYLLGTSNYWEEK-VMTLATLYAIKQIAWYLCFYGIHPAHL 780
                 L + KQKL++ +      G+ N+ E K  M L  LYA+KQ+A+YLCF+G+H AHL
Sbjct: 721  -----LGISKQKLLELITGEGSEGSYNHCEHKDKMELIVLYALKQVAYYLCFFGLHAAHL 780

Query: 781  YLKKLCQSLECLKSRLGFLASLIEDASKMVDREITISHPALTTIQDILCSKTSSSTLKVL 840
            Y+  L +SLE    RL  +   I +A +  +R++  SHP+L+ I+ IL S       K+L
Sbjct: 781  YISNLTRSLENTPERLKHILWSISEAQRKSERQLFESHPSLSCIETILRSNKQIDQ-KIL 840

Query: 841  VVANEIFWGSLKKLFGSMGLSFEELSFGGPTD--QQVFNANS---MVDELV-PNCLLVSQ 900
            +VA+  FW  L +   SM ++F E      T     V   NS   +++EL+  +C+L+  
Sbjct: 841  IVADRAFWLPLGQKLASMRMTFVEFGQNPATTFVDLVNKTNSTAWVLEELLKSDCILLDN 900

Query: 901  GHISGSFPFNKFSIILEYGGINGSSQISTYFTKSINMPHLHFVMLELEKCGNCKAFCKGV 960
             +I  SFPF+KF IILEYGG N SS + +   K   +P LHF+ ++++     K F   +
Sbjct: 901  KNIPASFPFDKFGIILEYGGPNKSSTLLSLAPKLDGLPPLHFLYVKVDG----KDFPAAL 960

Query: 961  GLPQHNELKIE----------EKSLVENQTRM-LEELLNFLP------------------ 1020
                H +  ++          +K L E   +M + + LNF+P                  
Sbjct: 961  VEDNHKDQDLKSTLDKVLLTLQKDLQERMNKMRIVDSLNFIPATNQLQGLQEKRSKHFAA 1020

Query: 1021 -----------------VDEKNILAPPEATIEAEDLKVPLQVSVGPV------------- 1080
                             +++KN        +  E L +   +S  PV             
Sbjct: 1021 DATKELLPDDQPHRLQNLNKKNTFDSHNVVLADEQLHIQQTLSNKPVVNSQCVPTVEKSS 1080

Query: 1081 --SGKSQHI-----------DL-------------VSFPEAIVIVNTQKFEKEMIVSRRS 1140
              S  S ++           DL             +S P+ +++VNT    K M+VSRRS
Sbjct: 1081 STSSVSANVLKDPQENQSTTDLPSCVKNDCIMPGRLSVPDVVIVVNTGNHGKTMLVSRRS 1140

Query: 1141 SYQRLLALEKDGVQVVERDLSFPVDLIITSGLCLMWYDCTNIHRKASTPNEASSCMNLCI 1200
            SYQ++LALEK G+QVVERD+  PVDLI+++ +CL+WY+         T +  +S +   +
Sbjct: 1141 SYQQILALEKGGMQVVERDIDLPVDLILSAAVCLVWYETALFEANELTTSAETSGIKENV 1200

Query: 1201 ENIATDVLTSLSFAFSGCILVFEGEISYLSIVMESSDGLYAAAASLEIDFQLFCSYSSEL 1260
            ENIAT++L S+SF+F+GCI+VFEGE  +LS VM+SSD LY AAASL+++ QLF S++   
Sbjct: 1201 ENIATNILMSVSFSFTGCIMVFEGEADFLSAVMDSSDSLYTAAASLDMNLQLFFSHTPRS 1260

Query: 1261 TDEIILGCIENVSKSMNRHLYPKMSESETLAESFLTSFPSINPLTAHGILSSESFLCDFL 1320
            TDEIIL CI NV+      L P + ESE+LAESFLTSFPSINP++A+ +LSS   L +FL
Sbjct: 1261 TDEIILNCITNVTSCYKAPL-PDIPESESLAESFLTSFPSINPVSAYMLLSSGGSLVEFL 1320

Query: 1321 EWPHERRLHAVKKYCIPDESVSLFSVLCKYGEREDSKSVMTDCSSSVSSGPDSDKCYFNG 1380
             WPHERR+ AV KY +  + +SLF+ LCK+GE  +S+SVMT+CSS  S   D    +   
Sbjct: 1321 SWPHERRIQAVGKYLLSPKIISLFNALCKFGELGESRSVMTECSSVDS---DISSAFLQS 1380

Query: 1381 NSKRKRRNFTGGTQCIDKNMDVFNSDAPNPFSAGSRETLSASKSLGSQMFEDQEIFSGLK 1440
              KRK+R+      C      +  SD+ N               +     E  E+FS  K
Sbjct: 1381 PRKRKQRSLQA---CAVPTNKLLFSDSLN--------------QIPGDYAEHAEVFSPSK 1440

Query: 1441 -GLSSSVNHFFDQNHNLESFDASISMNPNSFGKSRDSWISTSPEISNEIRRHCSSYVQNQ 1500
                S +++   +  ++ +FD S++M    F              S + ++H        
Sbjct: 1441 LRKFSDMDNTIPELPDVFTFDESLNMRSEGF--------------SYQQKKH-----DVD 1500

Query: 1501 GLDSNRRKRHNLHNMNKSENQ-HEELTGEVVSLIDDPVFKDDFASMAPMNFLPSMLDYEK 1560
             +  N+    +  N     NQ +   TG +V   D P ++ +F         PS   +  
Sbjct: 1501 AIPGNQVINDDFSNGLTPNNQAYNRRTGNMVDTFDLP-WQPEFGGTH-----PSKSTFHT 1560

Query: 1561 DSPRKSKIQRKLYHGQSDHPFSAVGMANNSSSDFWSPMNFHRQNSGGLDNHFPDPSFQPR 1620
              P  S+           +P  +     N     W+     +Q   GL +     +    
Sbjct: 1561 SRPSCSRTH--------SNPVFSTAFEINDDPGEWNISGGTKQTWKGLAH---GGTVDDS 1620

Query: 1621 YKDDHSDEGLMQNSVRDSKVLFSLSEKGMSH--SDVTPLSIALRSKHLQESSPWTMEFLN 1635
            Y+ D      M N   + +      ++ M H  S +    +   S    + S W +++L 
Sbjct: 1621 YRYD------MDNRYHEPR------DEIMQHPASSLAFQKLDFGSHATSQGSCWEIDYLR 1680

BLAST of Sed0022815 vs. ExPASy TrEMBL
Match: A0A6J1I9N8 (protein SHORTAGE IN CHIASMATA 1 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111470528 PE=4 SV=1)

HSP 1 Score: 2526.9 bits (6548), Expect = 0.0e+00
Identity = 1304/1666 (78.27%), Postives = 1429/1666 (85.77%), Query Frame = 0

Query: 1    MRTRFLDIDYFAAGNEPFRCLPVPHLASKPPLSTAGDLLHFDFLPEISLAIDRLAIDSAL 60
            MRTRFL+ID+FA GNE F  LPVPHL S  PLST GDLLHFDFLPEISL ID L+IDSA+
Sbjct: 1    MRTRFLNIDFFAPGNESFHRLPVPHLVSN-PLSTVGDLLHFDFLPEISLGIDSLSIDSAI 60

Query: 61   SKFFDDVLPRRIDDDNDYRD-----SRFQGSTERI--------RFLEEEAKCAYEDNVEG 120
            S+FFDDVLPRRI+DD+ YRD     S F GS ERI        R  EEEAK  YEDN+EG
Sbjct: 61   SRFFDDVLPRRIEDDDVYRDAGCQSSGFHGSAERIFSSESVGTRLREEEAKGTYEDNMEG 120

Query: 121  GWKNFGSETSETEFKAIGTVIRNRNLTYDMIQFETPQLDAFLENAFLFEREEVQILAEFP 180
             WKNFGSETSE EFK IGT IRNRN + DMIQFETPQLDAFLENAFLFE EEVQIL   P
Sbjct: 121  SWKNFGSETSEMEFKDIGTEIRNRNFSNDMIQFETPQLDAFLENAFLFEEEEVQILTAMP 180

Query: 181  EAKFNLETLNQGLLNCPSEVKESVYAIEGITSEYLLDQKSCLVEEDFAQDQKLLHRLTFP 240
            E +F+LETLNQGLL  PSEVKES+Y +E I SEYLLDQ+SCL E+DFAQDQKL H+ TFP
Sbjct: 181  EVEFDLETLNQGLLKYPSEVKESIYDVEIIPSEYLLDQESCLFEDDFAQDQKLSHQFTFP 240

Query: 241  FLEVDEMVLETSAFFSMQDELCFVLDNSESPHSIQDDNLLVNDEENLCSMKSDIEEFLLD 300
            FLEVDEMVLET AF S+QDEL F+L+N+ES H IQDDNLLVN+EE L  M+ D EEFL D
Sbjct: 241  FLEVDEMVLETLAFLSLQDELFFILENTESAHRIQDDNLLVNNEEYLSFMRCDAEEFLSD 300

Query: 301  HFLRPCAVSEVASPDISGG-SDLMSLMEILEIPVSSAVQTKPSCDLPVGLAIFEEFQLLD 360
            HFLRPCAVSE+ASPDISGG SDLMS+ME LEI VSS VQTK SCDL V  AIFEEFQLLD
Sbjct: 301  HFLRPCAVSELASPDISGGSSDLMSMMETLEIAVSSEVQTKSSCDLSVEPAIFEEFQLLD 360

Query: 361  IYSNQPFEAFFDLELSAVSEISDCMSIENMNFKSFNELIVCHELALVDDTFKSLPVPLLS 420
             YSNQ F   FDLE+SA+SEI+DC SIEN NFKSFNELIVCHEL LVDD FKSLPVP+LS
Sbjct: 361  TYSNQHFGVIFDLEVSAMSEITDCTSIENTNFKSFNELIVCHELVLVDDKFKSLPVPILS 420

Query: 421  SEESERPMFAFIEDVLANLNGKSLSTSDGIYLDWYLLEEDSCSFGIYSSYQNLLEEINLK 480
            S+ SE+P+ AFIEDVLANLN +SLS SDGIYLDW+LL+  S S G+YSSYQN+LEEINLK
Sbjct: 421  SQGSEKPLNAFIEDVLANLNKQSLSASDGIYLDWHLLDGSSYSSGLYSSYQNMLEEINLK 480

Query: 481  PVEFDQEPCGYDSIFYRYVFSDDALDRERTEDKGELKEPFPDGISMLPSQTIDVASSKLL 540
            PVEFD+EPC  D IFYRYVFSDDAL RERTEDKGELKE FP+GI ML  QTIDVAS KLL
Sbjct: 481  PVEFDREPCENDGIFYRYVFSDDALVRERTEDKGELKESFPEGIPMLSGQTIDVASRKLL 540

Query: 541  NDGCQQLGRQNLVAVGNTEKVLSSWKSQSELNDPKFFLNSQKPAGTRKGESVFSATDINT 600
            N+ CQQ  RQ L AVGN+EK LSSWKS+SE ND KFFLNSQKPAG RK ESVFSA D NT
Sbjct: 541  NERCQQKERQGLAAVGNSEKALSSWKSKSESNDLKFFLNSQKPAGARKSESVFSAIDTNT 600

Query: 601  ILPKVPCDGQLTNNPSTTSADVSLKQLNVTLHQVCLSDNILHLINNSEKTYLAILQNETE 660
             LPKV  DG LTN PS +SAD SLKQLNV +HQVCLSDNILHLINNSEKTYLAILQNETE
Sbjct: 601  TLPKVSRDGILTNKPSMSSADDSLKQLNVAVHQVCLSDNILHLINNSEKTYLAILQNETE 660

Query: 661  LRKTYLPYVAEDYVLMLSLPKQKLIDCVKKIYLLGTSNYWEEKVMTLATLYAIKQIAWYL 720
            LRK YLPYVAEDY+LMLSLPKQKLIDC+KKIYL GT+ YWEEK+MTLATLYA KQI WYL
Sbjct: 661  LRKKYLPYVAEDYLLMLSLPKQKLIDCIKKIYLQGTNTYWEEKIMTLATLYATKQIVWYL 720

Query: 721  CFYGIHPAHLYLKKLCQSLECLKSRLGFLASLIEDASKMVDREITISHPALTTIQDILCS 780
            CF+GIHPAHLYLKKLCQS ECLKSRLGFL S IE+A KMVD+EITISHPALTTIQ+ILCS
Sbjct: 721  CFFGIHPAHLYLKKLCQSSECLKSRLGFLVSSIEEAGKMVDKEITISHPALTTIQEILCS 780

Query: 781  KTSSSTLKVLVVANEIFWGSLKKLFGSMGLSFEELSFGGPTDQQVFNANSMVDELVPNCL 840
            +TS+S+LKVLVVAN+IFW SLK L GS+G+SF E+++G  TD+QV NAN+MVD+L  NCL
Sbjct: 781  RTSTSSLKVLVVANQIFWWSLKILLGSLGISFAEINYGSLTDEQVSNANAMVDDLASNCL 840

Query: 841  LVSQGHISGSFPFNKFSIILEYGGINGSSQISTYFTKSINMPHLHFVMLELEKCGNCKAF 900
            LVSQ +ISGSFPFNKFSI+LEYGG+NGSSQIS YF+  I+MPHLHF+MLEL+KCGNCKAF
Sbjct: 841  LVSQEYISGSFPFNKFSIVLEYGGLNGSSQISAYFSNLIDMPHLHFIMLELDKCGNCKAF 900

Query: 901  CKGVGLPQHNELKIEEKSLVENQTRMLEELLNFLPVDEKNILAPPEATIEAEDLKVPLQV 960
            C+GV  PQHNEL IEEKSLVENQT MLE+LLNFLPV+EK ILA  + TIEAE+ +VPL+ 
Sbjct: 901  CEGVDFPQHNELTIEEKSLVENQTMMLEKLLNFLPVEEKYILASSKKTIEAENRRVPLRA 960

Query: 961  SVGPVSGKSQHIDLVSFPEAIVIVNTQKFEKEMIVSRRSSYQRLLALEKDGVQVVERDLS 1020
             V PVS KSQH DLVSFPEAI+IVNTQKFEKEMIV RRSSYQR+LALEK+GVQVVERDL 
Sbjct: 961  PVVPVSDKSQHTDLVSFPEAIIIVNTQKFEKEMIVCRRSSYQRILALEKEGVQVVERDLC 1020

Query: 1021 FPVDLIITSGLCLMWYDCTNIHRKASTPNEASSCMNLCIENIATDVLTSLSFAFSGCILV 1080
             PVDLII  G+CL WYDCTNI RK ST NEAS C+NLCIENIATDVLTSLSFAF GC+LV
Sbjct: 1021 LPVDLIIAPGICLFWYDCTNIDRKGSTSNEASLCLNLCIENIATDVLTSLSFAFRGCVLV 1080

Query: 1081 FEGEISYLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKSMNRHLY 1140
            FEGEIS LSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSK M R +Y
Sbjct: 1081 FEGEISSLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKLMTRRIY 1140

Query: 1141 PKMSESETLAESFLTSFPSINPLTAHGILSSESFLCDFLEWPHERRLHAVKKYCIPDESV 1200
            PKMSESETLAESFLTSFPSINPL AHGILSSES L DFLEWPHERRLHA++KY IPD+ +
Sbjct: 1141 PKMSESETLAESFLTSFPSINPLIAHGILSSESLLADFLEWPHERRLHAIRKYSIPDDCI 1200

Query: 1201 SLFSVLCKYGEREDSKSVMTDCSSSVSSGPDSDKCYFNGNSKRKRRNFTGGTQCIDKNMD 1260
            SLFS LCKYGEREDSKSVMTDCSSS+SSG DS+KC+FNGNS+RKRRNFTGGTQCI+KN D
Sbjct: 1201 SLFSALCKYGEREDSKSVMTDCSSSLSSGADSEKCHFNGNSERKRRNFTGGTQCIEKNTD 1260

Query: 1261 VFNSDAPNPFSAGSRETLSASKSLGSQMFEDQEIFSGLKGLSSSVNHFFDQNHNLESFDA 1320
               S+  NPF+AG+ ETL ASKS GSQ+FED EIF  LKGLSSSVN+FFDQNHNL SFDA
Sbjct: 1261 CLYSNTLNPFTAGTAETLVASKSFGSQLFEDPEIFCDLKGLSSSVNNFFDQNHNLASFDA 1320

Query: 1321 SISMNPNSFGKSRDSWISTSPEISNEIRRHCSSYVQNQGLDSNRRKRHNLHNMNKSENQH 1380
            ++SM+P    K RD WIST+PEIS+EIRR CSS+VQNQGLD +++K HN HNMNKSEN H
Sbjct: 1321 TVSMDPTRVCKPRDCWISTAPEISDEIRRRCSSFVQNQGLDRSKKKIHNFHNMNKSENHH 1380

Query: 1381 EELTGEVVSLIDDPVFKDDFASMAPMNFLPSMLDYEKDSPRKSKIQRKLYHGQSDHPFSA 1440
            EEL  EV +LID+PV KD FA+MAPMNFLPSMLD E DS RKSKIQR+L +GQSDHPF A
Sbjct: 1381 EELIAEVDNLIDNPVLKDHFATMAPMNFLPSMLDNETDSSRKSKIQRRLSYGQSDHPFCA 1440

Query: 1441 VGMANNSSSDFWSPMNFHRQNSGGLDNHFPDPSFQP-----RYKDDHSDEGLMQNSVRDS 1500
            V + NNSSSDFWS +N H Q+S GLDN FPDPSF+P     RYKDDH DEGL+QN +RDS
Sbjct: 1441 VDVGNNSSSDFWSSINLHGQSSRGLDNQFPDPSFEPSIMPLRYKDDHLDEGLIQNPLRDS 1500

Query: 1501 KVLFSLSEKGMSHSDVTPLSIALRSKHLQESSPWTMEFLNRIKEKSRNRLHSVPCGSSSP 1560
            KV FSLSEK  SHSDVTPLS+ALRSKHLQESSPWTMEFLNRI+EKSRNR HSVP GSSSP
Sbjct: 1501 KVSFSLSEKDTSHSDVTPLSVALRSKHLQESSPWTMEFLNRIREKSRNRQHSVPRGSSSP 1560

Query: 1561 FPEHLGNVRKTVKRRSPSILEFFKYQGGSTQRKKPEQKRQKQSMQSSNSSKNVLVPTNEL 1620
            FPE+LGNV+KTVKRRSPSILEFFKYQGGST RKKPEQKR K S QSSNSSKNVLV T EL
Sbjct: 1561 FPENLGNVKKTVKRRSPSILEFFKYQGGSTLRKKPEQKRHKPSTQSSNSSKNVLVATREL 1620

Query: 1621 SSWTPIDKRSRQTLSFAIDGNERQTKLVWSNDNYGLGQSSQKLTNK 1648
            SS TPIDKRSRQTLS+A DGN  QTKLVWSNDNYGLG++SQKL NK
Sbjct: 1621 SSGTPIDKRSRQTLSYATDGNGSQTKLVWSNDNYGLGKNSQKLGNK 1665

BLAST of Sed0022815 vs. ExPASy TrEMBL
Match: A0A6J1I842 (protein SHORTAGE IN CHIASMATA 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470528 PE=4 SV=1)

HSP 1 Score: 2521.9 bits (6535), Expect = 0.0e+00
Identity = 1304/1668 (78.18%), Postives = 1429/1668 (85.67%), Query Frame = 0

Query: 1    MRTRFLDIDYFAAGNEPFRCLPVPHLASKPPLSTAGDLLHFDFLPEISLAIDRLAIDSAL 60
            MRTRFL+ID+FA GNE F  LPVPHL S  PLST GDLLHFDFLPEISL ID L+IDSA+
Sbjct: 1    MRTRFLNIDFFAPGNESFHRLPVPHLVSN-PLSTVGDLLHFDFLPEISLGIDSLSIDSAI 60

Query: 61   SKFFDDVLPRRIDDDNDYRD-----SRFQGSTERI--------RFLEEEAKCAYEDNVEG 120
            S+FFDDVLPRRI+DD+ YRD     S F GS ERI        R  EEEAK  YEDN+EG
Sbjct: 61   SRFFDDVLPRRIEDDDVYRDAGCQSSGFHGSAERIFSSESVGTRLREEEAKGTYEDNMEG 120

Query: 121  GWKNFGSETSETEF--KAIGTVIRNRNLTYDMIQFETPQLDAFLENAFLFEREEVQILAE 180
             WKNFGSETSE EF  K IGT IRNRN + DMIQFETPQLDAFLENAFLFE EEVQIL  
Sbjct: 121  SWKNFGSETSEMEFVKKDIGTEIRNRNFSNDMIQFETPQLDAFLENAFLFEEEEVQILTA 180

Query: 181  FPEAKFNLETLNQGLLNCPSEVKESVYAIEGITSEYLLDQKSCLVEEDFAQDQKLLHRLT 240
             PE +F+LETLNQGLL  PSEVKES+Y +E I SEYLLDQ+SCL E+DFAQDQKL H+ T
Sbjct: 181  MPEVEFDLETLNQGLLKYPSEVKESIYDVEIIPSEYLLDQESCLFEDDFAQDQKLSHQFT 240

Query: 241  FPFLEVDEMVLETSAFFSMQDELCFVLDNSESPHSIQDDNLLVNDEENLCSMKSDIEEFL 300
            FPFLEVDEMVLET AF S+QDEL F+L+N+ES H IQDDNLLVN+EE L  M+ D EEFL
Sbjct: 241  FPFLEVDEMVLETLAFLSLQDELFFILENTESAHRIQDDNLLVNNEEYLSFMRCDAEEFL 300

Query: 301  LDHFLRPCAVSEVASPDISGG-SDLMSLMEILEIPVSSAVQTKPSCDLPVGLAIFEEFQL 360
             DHFLRPCAVSE+ASPDISGG SDLMS+ME LEI VSS VQTK SCDL V  AIFEEFQL
Sbjct: 301  SDHFLRPCAVSELASPDISGGSSDLMSMMETLEIAVSSEVQTKSSCDLSVEPAIFEEFQL 360

Query: 361  LDIYSNQPFEAFFDLELSAVSEISDCMSIENMNFKSFNELIVCHELALVDDTFKSLPVPL 420
            LD YSNQ F   FDLE+SA+SEI+DC SIEN NFKSFNELIVCHEL LVDD FKSLPVP+
Sbjct: 361  LDTYSNQHFGVIFDLEVSAMSEITDCTSIENTNFKSFNELIVCHELVLVDDKFKSLPVPI 420

Query: 421  LSSEESERPMFAFIEDVLANLNGKSLSTSDGIYLDWYLLEEDSCSFGIYSSYQNLLEEIN 480
            LSS+ SE+P+ AFIEDVLANLN +SLS SDGIYLDW+LL+  S S G+YSSYQN+LEEIN
Sbjct: 421  LSSQGSEKPLNAFIEDVLANLNKQSLSASDGIYLDWHLLDGSSYSSGLYSSYQNMLEEIN 480

Query: 481  LKPVEFDQEPCGYDSIFYRYVFSDDALDRERTEDKGELKEPFPDGISMLPSQTIDVASSK 540
            LKPVEFD+EPC  D IFYRYVFSDDAL RERTEDKGELKE FP+GI ML  QTIDVAS K
Sbjct: 481  LKPVEFDREPCENDGIFYRYVFSDDALVRERTEDKGELKESFPEGIPMLSGQTIDVASRK 540

Query: 541  LLNDGCQQLGRQNLVAVGNTEKVLSSWKSQSELNDPKFFLNSQKPAGTRKGESVFSATDI 600
            LLN+ CQQ  RQ L AVGN+EK LSSWKS+SE ND KFFLNSQKPAG RK ESVFSA D 
Sbjct: 541  LLNERCQQKERQGLAAVGNSEKALSSWKSKSESNDLKFFLNSQKPAGARKSESVFSAIDT 600

Query: 601  NTILPKVPCDGQLTNNPSTTSADVSLKQLNVTLHQVCLSDNILHLINNSEKTYLAILQNE 660
            NT LPKV  DG LTN PS +SAD SLKQLNV +HQVCLSDNILHLINNSEKTYLAILQNE
Sbjct: 601  NTTLPKVSRDGILTNKPSMSSADDSLKQLNVAVHQVCLSDNILHLINNSEKTYLAILQNE 660

Query: 661  TELRKTYLPYVAEDYVLMLSLPKQKLIDCVKKIYLLGTSNYWEEKVMTLATLYAIKQIAW 720
            TELRK YLPYVAEDY+LMLSLPKQKLIDC+KKIYL GT+ YWEEK+MTLATLYA KQI W
Sbjct: 661  TELRKKYLPYVAEDYLLMLSLPKQKLIDCIKKIYLQGTNTYWEEKIMTLATLYATKQIVW 720

Query: 721  YLCFYGIHPAHLYLKKLCQSLECLKSRLGFLASLIEDASKMVDREITISHPALTTIQDIL 780
            YLCF+GIHPAHLYLKKLCQS ECLKSRLGFL S IE+A KMVD+EITISHPALTTIQ+IL
Sbjct: 721  YLCFFGIHPAHLYLKKLCQSSECLKSRLGFLVSSIEEAGKMVDKEITISHPALTTIQEIL 780

Query: 781  CSKTSSSTLKVLVVANEIFWGSLKKLFGSMGLSFEELSFGGPTDQQVFNANSMVDELVPN 840
            CS+TS+S+LKVLVVAN+IFW SLK L GS+G+SF E+++G  TD+QV NAN+MVD+L  N
Sbjct: 781  CSRTSTSSLKVLVVANQIFWWSLKILLGSLGISFAEINYGSLTDEQVSNANAMVDDLASN 840

Query: 841  CLLVSQGHISGSFPFNKFSIILEYGGINGSSQISTYFTKSINMPHLHFVMLELEKCGNCK 900
            CLLVSQ +ISGSFPFNKFSI+LEYGG+NGSSQIS YF+  I+MPHLHF+MLEL+KCGNCK
Sbjct: 841  CLLVSQEYISGSFPFNKFSIVLEYGGLNGSSQISAYFSNLIDMPHLHFIMLELDKCGNCK 900

Query: 901  AFCKGVGLPQHNELKIEEKSLVENQTRMLEELLNFLPVDEKNILAPPEATIEAEDLKVPL 960
            AFC+GV  PQHNEL IEEKSLVENQT MLE+LLNFLPV+EK ILA  + TIEAE+ +VPL
Sbjct: 901  AFCEGVDFPQHNELTIEEKSLVENQTMMLEKLLNFLPVEEKYILASSKKTIEAENRRVPL 960

Query: 961  QVSVGPVSGKSQHIDLVSFPEAIVIVNTQKFEKEMIVSRRSSYQRLLALEKDGVQVVERD 1020
            +  V PVS KSQH DLVSFPEAI+IVNTQKFEKEMIV RRSSYQR+LALEK+GVQVVERD
Sbjct: 961  RAPVVPVSDKSQHTDLVSFPEAIIIVNTQKFEKEMIVCRRSSYQRILALEKEGVQVVERD 1020

Query: 1021 LSFPVDLIITSGLCLMWYDCTNIHRKASTPNEASSCMNLCIENIATDVLTSLSFAFSGCI 1080
            L  PVDLII  G+CL WYDCTNI RK ST NEAS C+NLCIENIATDVLTSLSFAF GC+
Sbjct: 1021 LCLPVDLIIAPGICLFWYDCTNIDRKGSTSNEASLCLNLCIENIATDVLTSLSFAFRGCV 1080

Query: 1081 LVFEGEISYLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKSMNRH 1140
            LVFEGEIS LSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSK M R 
Sbjct: 1081 LVFEGEISSLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKLMTRR 1140

Query: 1141 LYPKMSESETLAESFLTSFPSINPLTAHGILSSESFLCDFLEWPHERRLHAVKKYCIPDE 1200
            +YPKMSESETLAESFLTSFPSINPL AHGILSSES L DFLEWPHERRLHA++KY IPD+
Sbjct: 1141 IYPKMSESETLAESFLTSFPSINPLIAHGILSSESLLADFLEWPHERRLHAIRKYSIPDD 1200

Query: 1201 SVSLFSVLCKYGEREDSKSVMTDCSSSVSSGPDSDKCYFNGNSKRKRRNFTGGTQCIDKN 1260
             +SLFS LCKYGEREDSKSVMTDCSSS+SSG DS+KC+FNGNS+RKRRNFTGGTQCI+KN
Sbjct: 1201 CISLFSALCKYGEREDSKSVMTDCSSSLSSGADSEKCHFNGNSERKRRNFTGGTQCIEKN 1260

Query: 1261 MDVFNSDAPNPFSAGSRETLSASKSLGSQMFEDQEIFSGLKGLSSSVNHFFDQNHNLESF 1320
             D   S+  NPF+AG+ ETL ASKS GSQ+FED EIF  LKGLSSSVN+FFDQNHNL SF
Sbjct: 1261 TDCLYSNTLNPFTAGTAETLVASKSFGSQLFEDPEIFCDLKGLSSSVNNFFDQNHNLASF 1320

Query: 1321 DASISMNPNSFGKSRDSWISTSPEISNEIRRHCSSYVQNQGLDSNRRKRHNLHNMNKSEN 1380
            DA++SM+P    K RD WIST+PEIS+EIRR CSS+VQNQGLD +++K HN HNMNKSEN
Sbjct: 1321 DATVSMDPTRVCKPRDCWISTAPEISDEIRRRCSSFVQNQGLDRSKKKIHNFHNMNKSEN 1380

Query: 1381 QHEELTGEVVSLIDDPVFKDDFASMAPMNFLPSMLDYEKDSPRKSKIQRKLYHGQSDHPF 1440
             HEEL  EV +LID+PV KD FA+MAPMNFLPSMLD E DS RKSKIQR+L +GQSDHPF
Sbjct: 1381 HHEELIAEVDNLIDNPVLKDHFATMAPMNFLPSMLDNETDSSRKSKIQRRLSYGQSDHPF 1440

Query: 1441 SAVGMANNSSSDFWSPMNFHRQNSGGLDNHFPDPSFQP-----RYKDDHSDEGLMQNSVR 1500
             AV + NNSSSDFWS +N H Q+S GLDN FPDPSF+P     RYKDDH DEGL+QN +R
Sbjct: 1441 CAVDVGNNSSSDFWSSINLHGQSSRGLDNQFPDPSFEPSIMPLRYKDDHLDEGLIQNPLR 1500

Query: 1501 DSKVLFSLSEKGMSHSDVTPLSIALRSKHLQESSPWTMEFLNRIKEKSRNRLHSVPCGSS 1560
            DSKV FSLSEK  SHSDVTPLS+ALRSKHLQESSPWTMEFLNRI+EKSRNR HSVP GSS
Sbjct: 1501 DSKVSFSLSEKDTSHSDVTPLSVALRSKHLQESSPWTMEFLNRIREKSRNRQHSVPRGSS 1560

Query: 1561 SPFPEHLGNVRKTVKRRSPSILEFFKYQGGSTQRKKPEQKRQKQSMQSSNSSKNVLVPTN 1620
            SPFPE+LGNV+KTVKRRSPSILEFFKYQGGST RKKPEQKR K S QSSNSSKNVLV T 
Sbjct: 1561 SPFPENLGNVKKTVKRRSPSILEFFKYQGGSTLRKKPEQKRHKPSTQSSNSSKNVLVATR 1620

Query: 1621 ELSSWTPIDKRSRQTLSFAIDGNERQTKLVWSNDNYGLGQSSQKLTNK 1648
            ELSS TPIDKRSRQTLS+A DGN  QTKLVWSNDNYGLG++SQKL NK
Sbjct: 1621 ELSSGTPIDKRSRQTLSYATDGNGSQTKLVWSNDNYGLGKNSQKLGNK 1667

BLAST of Sed0022815 vs. ExPASy TrEMBL
Match: A0A6J1GJU9 (protein SHORTAGE IN CHIASMATA 1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111454972 PE=4 SV=1)

HSP 1 Score: 2516.9 bits (6522), Expect = 0.0e+00
Identity = 1299/1666 (77.97%), Postives = 1427/1666 (85.65%), Query Frame = 0

Query: 1    MRTRFLDIDYFAAGNEPFRCLPVPHLASKPPLSTAGDLLHFDFLPEISLAIDRLAIDSAL 60
            MRTRFL+ID+FA GNE F  LPVPHL S  PLST GDLLHFDFLPEISL ID L+IDSA+
Sbjct: 1    MRTRFLNIDFFAPGNESFHRLPVPHLVSN-PLSTVGDLLHFDFLPEISLGIDSLSIDSAI 60

Query: 61   SKFFDDVLPRRIDDDNDYRD-----SRFQGSTERI--------RFLEEEAKCAYEDNVEG 120
            S+FFDDVLPRRI DD+ YRD     S F GS ERI        R  EEEAK  YEDN+EG
Sbjct: 61   SRFFDDVLPRRIVDDDVYRDAGCQSSGFHGSAERIFSSESVGTRLREEEAKGTYEDNMEG 120

Query: 121  GWKNFGSETSETEFKAIGTVIRNRNLTYDMIQFETPQLDAFLENAFLFEREEVQILAEFP 180
             WKNFGSETSE EFK IGT IRNRN + DMIQFETPQLD FLENAFLFE+EEVQIL   P
Sbjct: 121  SWKNFGSETSEMEFKDIGTEIRNRNFSNDMIQFETPQLDTFLENAFLFEKEEVQILTAMP 180

Query: 181  EAKFNLETLNQGLLNCPSEVKESVYAIEGITSEYLLDQKSCLVEEDFAQDQKLLHRLTFP 240
            E +F+LETLNQGLL  PSEVKES+Y +E I SEYLLDQ+SCL E+DFAQDQKL H+ TFP
Sbjct: 181  EVEFDLETLNQGLLKYPSEVKESIYDVEIIPSEYLLDQESCLFEDDFAQDQKLSHQFTFP 240

Query: 241  FLEVDEMVLETSAFFSMQDELCFVLDNSESPHSIQDDNLLVNDEENLCSMKSDIEEFLLD 300
            FLEVDEMVLET AF S+QDEL F+L+++ES H I DDNLLVN+EE L SM+ D EEFL D
Sbjct: 241  FLEVDEMVLETLAFLSLQDELFFILESTESAHMIHDDNLLVNNEEYLSSMRCDAEEFLSD 300

Query: 301  HFLRPCAVSEVASPDISGG-SDLMSLMEILEIPVSSAVQTKPSCDLPVGLAIFEEFQLLD 360
            HFLRPC VSE+ASPDISGG SDLMS+ME LEI VSS VQTK SCDL V  AIFEEFQLLD
Sbjct: 301  HFLRPCGVSELASPDISGGSSDLMSMMETLEIAVSSEVQTKSSCDLSVEPAIFEEFQLLD 360

Query: 361  IYSNQPFEAFFDLELSAVSEISDCMSIENMNFKSFNELIVCHELALVDDTFKSLPVPLLS 420
             YSNQ F A FDLE+SA+SEI+DC SIEN NFKSFNELIVCHEL LVDDTFKSLPVP+LS
Sbjct: 361  TYSNQHFGAIFDLEVSAMSEITDCTSIENTNFKSFNELIVCHELVLVDDTFKSLPVPILS 420

Query: 421  SEESERPMFAFIEDVLANLNGKSLSTSDGIYLDWYLLEEDSCSFGIYSSYQNLLEEINLK 480
            S+ SE+ + AFIEDVLANLN +SLS SDGIYLDW+LL+E S S G+YSSYQN+LEEINLK
Sbjct: 421  SQGSEKLLNAFIEDVLANLNKQSLSASDGIYLDWHLLDESSYSSGLYSSYQNMLEEINLK 480

Query: 481  PVEFDQEPCGYDSIFYRYVFSDDALDRERTEDKGELKEPFPDGISMLPSQTIDVASSKLL 540
            PVEFD+EPC  D IFYRYVFSDDAL RERTEDKGELKE FP+GI ML  QTIDVASS LL
Sbjct: 481  PVEFDREPCENDGIFYRYVFSDDALVRERTEDKGELKESFPEGIPMLSGQTIDVASSILL 540

Query: 541  NDGCQQLGRQNLVAVGNTEKVLSSWKSQSELNDPKFFLNSQKPAGTRKGESVFSATDINT 600
            N+ CQQ  RQ L AVGN+EK LSSWKS+SE ND KFFLNSQKP G RK ESVFS  D NT
Sbjct: 541  NERCQQKERQGLAAVGNSEKALSSWKSKSESNDLKFFLNSQKPVGVRKSESVFSEIDTNT 600

Query: 601  ILPKVPCDGQLTNNPSTTSADVSLKQLNVTLHQVCLSDNILHLINNSEKTYLAILQNETE 660
             LPKVP DG LTN PS +SAD SLKQLNV +HQVCLSDNILHLINNSEKTYLAILQNETE
Sbjct: 601  TLPKVPRDGILTNKPSMSSADDSLKQLNVAVHQVCLSDNILHLINNSEKTYLAILQNETE 660

Query: 661  LRKTYLPYVAEDYVLMLSLPKQKLIDCVKKIYLLGTSNYWEEKVMTLATLYAIKQIAWYL 720
            LRK YLPYVAEDY+LMLSLPKQKLIDC+KKIYL GT+ YWEEK+MTLATLYA KQI WYL
Sbjct: 661  LRKKYLPYVAEDYLLMLSLPKQKLIDCIKKIYLQGTNTYWEEKIMTLATLYATKQIVWYL 720

Query: 721  CFYGIHPAHLYLKKLCQSLECLKSRLGFLASLIEDASKMVDREITISHPALTTIQDILCS 780
            CFYGIHPAHLYLKKLCQS ECLKSRLGFL S IE+A KMV REITISHPALTTIQ+ILCS
Sbjct: 721  CFYGIHPAHLYLKKLCQSSECLKSRLGFLVSSIEEAGKMVGREITISHPALTTIQEILCS 780

Query: 781  KTSSSTLKVLVVANEIFWGSLKKLFGSMGLSFEELSFGGPTDQQVFNANSMVDELVPNCL 840
            +TS+S+LKVLVVAN+IFW SL+KL GS+G+SF E+++G  T +QV NAN+MVD+L  NCL
Sbjct: 781  RTSTSSLKVLVVANQIFWWSLEKLLGSLGISFAEINYGSLTVEQVSNANAMVDDLASNCL 840

Query: 841  LVSQGHISGSFPFNKFSIILEYGGINGSSQISTYFTKSINMPHLHFVMLELEKCGNCKAF 900
            LVSQ +ISGSFPFNKFSI+LEYGG+NGSSQIS YF+  I+MPHLHF+MLEL+KCGNCKAF
Sbjct: 841  LVSQEYISGSFPFNKFSIVLEYGGLNGSSQISAYFSNLIDMPHLHFIMLELDKCGNCKAF 900

Query: 901  CKGVGLPQHNELKIEEKSLVENQTRMLEELLNFLPVDEKNILAPPEATIEAEDLKVPLQV 960
            C+GV  PQHNEL IEEKSLVENQT MLE+LLNFLPV+EK ILA P+ TIEAE+ +VPL+ 
Sbjct: 901  CEGVDFPQHNELTIEEKSLVENQTMMLEKLLNFLPVEEKYILASPKKTIEAENRRVPLRA 960

Query: 961  SVGPVSGKSQHIDLVSFPEAIVIVNTQKFEKEMIVSRRSSYQRLLALEKDGVQVVERDLS 1020
             V PVS KSQ+ DLVSFPEAI+IVNTQKFEKEMIV RRSSY+R+LALEK+GVQVVERDL 
Sbjct: 961  PVEPVSDKSQYTDLVSFPEAIIIVNTQKFEKEMIVCRRSSYRRILALEKEGVQVVERDLC 1020

Query: 1021 FPVDLIITSGLCLMWYDCTNIHRKASTPNEASSCMNLCIENIATDVLTSLSFAFSGCILV 1080
             PVDLII  G+CL WYDCTNI RK ST NEAS C+NLCIENIATDVLTSLSFAF GC+LV
Sbjct: 1021 LPVDLIIAPGICLFWYDCTNIDRKGSTSNEASLCLNLCIENIATDVLTSLSFAFRGCVLV 1080

Query: 1081 FEGEISYLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKSMNRHLY 1140
            FEGEIS LSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSK M R +Y
Sbjct: 1081 FEGEISSLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKLMTRRIY 1140

Query: 1141 PKMSESETLAESFLTSFPSINPLTAHGILSSESFLCDFLEWPHERRLHAVKKYCIPDESV 1200
            PKMSESETLAESFLTSFPSINPL AHGILSSES L DFLEWPHERRLHA++KY IPD+ +
Sbjct: 1141 PKMSESETLAESFLTSFPSINPLIAHGILSSESLLADFLEWPHERRLHAIRKYSIPDDCI 1200

Query: 1201 SLFSVLCKYGEREDSKSVMTDCSSSVSSGPDSDKCYFNGNSKRKRRNFTGGTQCIDKNMD 1260
            SLFS LCKYGEREDSKSVMTDCSSS+SSG DS+KC+FNGNS+RKRRNFTGGTQCI+KN D
Sbjct: 1201 SLFSALCKYGEREDSKSVMTDCSSSLSSGADSEKCHFNGNSERKRRNFTGGTQCIEKNTD 1260

Query: 1261 VFNSDAPNPFSAGSRETLSASKSLGSQMFEDQEIFSGLKGLSSSVNHFFDQNHNLESFDA 1320
               S+  NPF+AG+ ETL ASKS GSQ+FED EIF  LKGLSSSVN+FFDQNHNL SFDA
Sbjct: 1261 CLYSNTLNPFTAGTAETLVASKSFGSQLFEDPEIFCDLKGLSSSVNNFFDQNHNLASFDA 1320

Query: 1321 SISMNPNSFGKSRDSWISTSPEISNEIRRHCSSYVQNQGLDSNRRKRHNLHNMNKSENQH 1380
            ++SM+P    K RD WIST+PEIS+EIRRHCS +VQNQGLD +++K H+ HNMNKSEN H
Sbjct: 1321 TVSMDPTRVCKPRDCWISTAPEISDEIRRHCSPFVQNQGLDRSKKKIHSFHNMNKSENHH 1380

Query: 1381 EELTGEVVSLIDDPVFKDDFASMAPMNFLPSMLDYEKDSPRKSKIQRKLYHGQSDHPFSA 1440
            EEL  EV +LID+PV KD FA+MAPMNFLPSML+ E DS RKSKIQR+L +GQSDHPF A
Sbjct: 1381 EELIAEVDNLIDNPVLKDHFATMAPMNFLPSMLENETDSSRKSKIQRRLSYGQSDHPFCA 1440

Query: 1441 VGMANNSSSDFWSPMNFHRQNSGGLDNHFPDPSFQP-----RYKDDHSDEGLMQNSVRDS 1500
            V + NNSSSDFWS +N H Q+S GLDN FPDPSF+P     RYKDDH DEGL QN +RDS
Sbjct: 1441 VDVGNNSSSDFWSSINLHGQSSRGLDNQFPDPSFEPSIMPLRYKDDHLDEGLTQNHLRDS 1500

Query: 1501 KVLFSLSEKGMSHSDVTPLSIALRSKHLQESSPWTMEFLNRIKEKSRNRLHSVPCGSSSP 1560
            KV FSLSEK  SHSDVTPLS+ALRSKHLQESSPWTMEFLNRI+EKSRNR HSVP GSSSP
Sbjct: 1501 KVSFSLSEKDTSHSDVTPLSVALRSKHLQESSPWTMEFLNRIREKSRNRQHSVPRGSSSP 1560

Query: 1561 FPEHLGNVRKTVKRRSPSILEFFKYQGGSTQRKKPEQKRQKQSMQSSNSSKNVLVPTNEL 1620
            FPE+LGNV+KTVKRRSPSILEFFKYQGGST RKKPEQKR K S QSSNSSKNVLV T EL
Sbjct: 1561 FPENLGNVKKTVKRRSPSILEFFKYQGGSTLRKKPEQKRHKPSTQSSNSSKNVLVATREL 1620

Query: 1621 SSWTPIDKRSRQTLSFAIDGNERQTKLVWSNDNYGLGQSSQKLTNK 1648
            SS TPIDKRSRQTLS+A DGN  QTKLVWSNDNYGLG++SQKL NK
Sbjct: 1621 SSGTPIDKRSRQTLSYATDGNGSQTKLVWSNDNYGLGKNSQKLGNK 1665

BLAST of Sed0022815 vs. ExPASy TrEMBL
Match: A0A6J1I320 (protein SHORTAGE IN CHIASMATA 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470528 PE=4 SV=1)

HSP 1 Score: 2516.1 bits (6520), Expect = 0.0e+00
Identity = 1304/1672 (77.99%), Postives = 1429/1672 (85.47%), Query Frame = 0

Query: 1    MRTRFLDIDYFAAGNEPFRCLPVPHLASKPPLSTAGDLLHFDFLPEISLAIDRLAIDSAL 60
            MRTRFL+ID+FA GNE F  LPVPHL S  PLST GDLLHFDFLPEISL ID L+IDSA+
Sbjct: 1    MRTRFLNIDFFAPGNESFHRLPVPHLVSN-PLSTVGDLLHFDFLPEISLGIDSLSIDSAI 60

Query: 61   SKFFDDVLPRRIDDDNDYRD-----SRFQGSTERI--------RFLEEEAKCAYEDNVEG 120
            S+FFDDVLPRRI+DD+ YRD     S F GS ERI        R  EEEAK  YEDN+EG
Sbjct: 61   SRFFDDVLPRRIEDDDVYRDAGCQSSGFHGSAERIFSSESVGTRLREEEAKGTYEDNMEG 120

Query: 121  GWKNFGSETSETEF--KAIGTVIRNRNLTYDMIQFETPQLDAFLENAFLFEREEVQILAE 180
             WKNFGSETSE EF  K IGT IRNRN + DMIQFETPQLDAFLENAFLFE EEVQIL  
Sbjct: 121  SWKNFGSETSEMEFVKKDIGTEIRNRNFSNDMIQFETPQLDAFLENAFLFEEEEVQILTA 180

Query: 181  FPEAKFNLETLNQGLLNCPSEVKESVYAIEGITSEYLLDQKSCLVEEDFAQDQKLLHRLT 240
             PE +F+LETLNQGLL  PSEVKES+Y +E I SEYLLDQ+SCL E+DFAQDQKL H+ T
Sbjct: 181  MPEVEFDLETLNQGLLKYPSEVKESIYDVEIIPSEYLLDQESCLFEDDFAQDQKLSHQFT 240

Query: 241  FPFLEVDEMVLETSAFFSMQDELCFVLDNSESPHSIQDDNLLVNDEENLCSMKSDIEEFL 300
            FPFLEVDEMVLET AF S+QDEL F+L+N+ES H IQDDNLLVN+EE L  M+ D EEFL
Sbjct: 241  FPFLEVDEMVLETLAFLSLQDELFFILENTESAHRIQDDNLLVNNEEYLSFMRCDAEEFL 300

Query: 301  LDHFLRPCAVSEVASPDISGG-SDLMSLMEILEIPVSSAVQTKPSCDLPVGLAIFEEFQL 360
             DHFLRPCAVSE+ASPDISGG SDLMS+ME LEI VSS VQTK SCDL V  AIFEEFQL
Sbjct: 301  SDHFLRPCAVSELASPDISGGSSDLMSMMETLEIAVSSEVQTKSSCDLSVEPAIFEEFQL 360

Query: 361  LDIYSNQPFEAFFDLELSAVSEISDCMSIENMNFKSFNELIVCHELALVDDTFKSLPVPL 420
            LD YSNQ F   FDLE+SA+SEI+DC SIEN NFKSFNELIVCHEL LVDD FKSLPVP+
Sbjct: 361  LDTYSNQHFGVIFDLEVSAMSEITDCTSIENTNFKSFNELIVCHELVLVDDKFKSLPVPI 420

Query: 421  LSSEESERPMFAFIEDVLANLNGKSLSTSDGIYLDWYLLEEDSCSFGIYSSYQNLLEEIN 480
            LSS+ SE+P+ AFIEDVLANLN +SLS SDGIYLDW+LL+  S S G+YSSYQN+LEEIN
Sbjct: 421  LSSQGSEKPLNAFIEDVLANLNKQSLSASDGIYLDWHLLDGSSYSSGLYSSYQNMLEEIN 480

Query: 481  LKPVEFDQEPCGYDSIFYRYVFSDDALDRERTEDKGELKEPFPDGISMLPSQTIDVASSK 540
            LKPVEFD+EPC  D IFYRYVFSDDAL RERTEDKGELKE FP+GI ML  QTIDVAS K
Sbjct: 481  LKPVEFDREPCENDGIFYRYVFSDDALVRERTEDKGELKESFPEGIPMLSGQTIDVASRK 540

Query: 541  LLNDGCQQLGRQNLVAVGNTEKVLSSWKSQSELNDPKFFLNSQKPAGTRKGESVFSATDI 600
            LLN+ CQQ  RQ L AVGN+EK LSSWKS+SE ND KFFLNSQKPAG RK ESVFSA D 
Sbjct: 541  LLNERCQQKERQGLAAVGNSEKALSSWKSKSESNDLKFFLNSQKPAGARKSESVFSAIDT 600

Query: 601  NTILPKVPCDGQLTNNPSTTSADVSLKQLNVTLHQVCLSDNILHLINNSEKTYLAILQNE 660
            NT LPKV  DG LTN PS +SAD SLKQLNV +HQVCLSDNILHLINNSEKTYLAILQNE
Sbjct: 601  NTTLPKVSRDGILTNKPSMSSADDSLKQLNVAVHQVCLSDNILHLINNSEKTYLAILQNE 660

Query: 661  TELRKTYLPYVAEDYVLMLSLPKQKLIDCVKKIYLLGTSNYWEEKVMTLATLYAIKQIAW 720
            TELRK YLPYVAEDY+LMLSLPKQKLIDC+KKIYL GT+ YWEEK+MTLATLYA KQI W
Sbjct: 661  TELRKKYLPYVAEDYLLMLSLPKQKLIDCIKKIYLQGTNTYWEEKIMTLATLYATKQIVW 720

Query: 721  YLCFYGIHPAHLYLKKLCQSLECLKSRLGFLASLIEDASKMVDREITISHPALTTIQDIL 780
            YLCF+GIHPAHLYLKKLCQS ECLKSRLGFL S IE+A KMVD+EITISHPALTTIQ+IL
Sbjct: 721  YLCFFGIHPAHLYLKKLCQSSECLKSRLGFLVSSIEEAGKMVDKEITISHPALTTIQEIL 780

Query: 781  CSKTSSSTLKVLVVANEIFWGSLKKLFGSMGLSFEELSFGGPTDQQVFNANSMVDELVPN 840
            CS+TS+S+LKVLVVAN+IFW SLK L GS+G+SF E+++G  TD+QV NAN+MVD+L  N
Sbjct: 781  CSRTSTSSLKVLVVANQIFWWSLKILLGSLGISFAEINYGSLTDEQVSNANAMVDDLASN 840

Query: 841  CLLVSQGHISGSFPFNKFSIILEYGGINGSSQISTYFTKSINMPHLHFVMLELEKCGNCK 900
            CLLVSQ +ISGSFPFNKFSI+LEYGG+NGSSQIS YF+  I+MPHLHF+MLEL+KCGNCK
Sbjct: 841  CLLVSQEYISGSFPFNKFSIVLEYGGLNGSSQISAYFSNLIDMPHLHFIMLELDKCGNCK 900

Query: 901  AFCKGVGLPQHNELKIEEKSLVENQTRMLEELLNFLPVDEKNILAPPEATIEAEDLKVPL 960
            AFC+GV  PQHNEL IEEKSLVENQT MLE+LLNFLPV+EK ILA  + TIEAE+ +VPL
Sbjct: 901  AFCEGVDFPQHNELTIEEKSLVENQTMMLEKLLNFLPVEEKYILASSKKTIEAENRRVPL 960

Query: 961  QVSVGPVSGKSQHIDLVSFPEAIVIVNTQKFEKEMIVSRRSSYQRLLALEKDGVQVVERD 1020
            +  V PVS KSQH DLVSFPEAI+IVNTQKFEKEMIV RRSSYQR+LALEK+GVQVVERD
Sbjct: 961  RAPVVPVSDKSQHTDLVSFPEAIIIVNTQKFEKEMIVCRRSSYQRILALEKEGVQVVERD 1020

Query: 1021 LSFPVDLIITSGLCLMWYDCTNIHRKASTPNEASSCMNLCIENIATDVLTSLSFAFSGCI 1080
            L  PVDLII  G+CL WYDCTNI RK ST NEAS C+NLCIENIATDVLTSLSFAF GC+
Sbjct: 1021 LCLPVDLIIAPGICLFWYDCTNIDRKGSTSNEASLCLNLCIENIATDVLTSLSFAFRGCV 1080

Query: 1081 L----VFEGEISYLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKS 1140
            L    VFEGEIS LSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSK 
Sbjct: 1081 LISFQVFEGEISSLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKL 1140

Query: 1141 MNRHLYPKMSESETLAESFLTSFPSINPLTAHGILSSESFLCDFLEWPHERRLHAVKKYC 1200
            M R +YPKMSESETLAESFLTSFPSINPL AHGILSSES L DFLEWPHERRLHA++KY 
Sbjct: 1141 MTRRIYPKMSESETLAESFLTSFPSINPLIAHGILSSESLLADFLEWPHERRLHAIRKYS 1200

Query: 1201 IPDESVSLFSVLCKYGEREDSKSVMTDCSSSVSSGPDSDKCYFNGNSKRKRRNFTGGTQC 1260
            IPD+ +SLFS LCKYGEREDSKSVMTDCSSS+SSG DS+KC+FNGNS+RKRRNFTGGTQC
Sbjct: 1201 IPDDCISLFSALCKYGEREDSKSVMTDCSSSLSSGADSEKCHFNGNSERKRRNFTGGTQC 1260

Query: 1261 IDKNMDVFNSDAPNPFSAGSRETLSASKSLGSQMFEDQEIFSGLKGLSSSVNHFFDQNHN 1320
            I+KN D   S+  NPF+AG+ ETL ASKS GSQ+FED EIF  LKGLSSSVN+FFDQNHN
Sbjct: 1261 IEKNTDCLYSNTLNPFTAGTAETLVASKSFGSQLFEDPEIFCDLKGLSSSVNNFFDQNHN 1320

Query: 1321 LESFDASISMNPNSFGKSRDSWISTSPEISNEIRRHCSSYVQNQGLDSNRRKRHNLHNMN 1380
            L SFDA++SM+P    K RD WIST+PEIS+EIRR CSS+VQNQGLD +++K HN HNMN
Sbjct: 1321 LASFDATVSMDPTRVCKPRDCWISTAPEISDEIRRRCSSFVQNQGLDRSKKKIHNFHNMN 1380

Query: 1381 KSENQHEELTGEVVSLIDDPVFKDDFASMAPMNFLPSMLDYEKDSPRKSKIQRKLYHGQS 1440
            KSEN HEEL  EV +LID+PV KD FA+MAPMNFLPSMLD E DS RKSKIQR+L +GQS
Sbjct: 1381 KSENHHEELIAEVDNLIDNPVLKDHFATMAPMNFLPSMLDNETDSSRKSKIQRRLSYGQS 1440

Query: 1441 DHPFSAVGMANNSSSDFWSPMNFHRQNSGGLDNHFPDPSFQP-----RYKDDHSDEGLMQ 1500
            DHPF AV + NNSSSDFWS +N H Q+S GLDN FPDPSF+P     RYKDDH DEGL+Q
Sbjct: 1441 DHPFCAVDVGNNSSSDFWSSINLHGQSSRGLDNQFPDPSFEPSIMPLRYKDDHLDEGLIQ 1500

Query: 1501 NSVRDSKVLFSLSEKGMSHSDVTPLSIALRSKHLQESSPWTMEFLNRIKEKSRNRLHSVP 1560
            N +RDSKV FSLSEK  SHSDVTPLS+ALRSKHLQESSPWTMEFLNRI+EKSRNR HSVP
Sbjct: 1501 NPLRDSKVSFSLSEKDTSHSDVTPLSVALRSKHLQESSPWTMEFLNRIREKSRNRQHSVP 1560

Query: 1561 CGSSSPFPEHLGNVRKTVKRRSPSILEFFKYQGGSTQRKKPEQKRQKQSMQSSNSSKNVL 1620
             GSSSPFPE+LGNV+KTVKRRSPSILEFFKYQGGST RKKPEQKR K S QSSNSSKNVL
Sbjct: 1561 RGSSSPFPENLGNVKKTVKRRSPSILEFFKYQGGSTLRKKPEQKRHKPSTQSSNSSKNVL 1620

Query: 1621 VPTNELSSWTPIDKRSRQTLSFAIDGNERQTKLVWSNDNYGLGQSSQKLTNK 1648
            V T ELSS TPIDKRSRQTLS+A DGN  QTKLVWSNDNYGLG++SQKL NK
Sbjct: 1621 VATRELSSGTPIDKRSRQTLSYATDGNGSQTKLVWSNDNYGLGKNSQKLGNK 1671

BLAST of Sed0022815 vs. ExPASy TrEMBL
Match: A0A6J1GJP5 (protein SHORTAGE IN CHIASMATA 1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454972 PE=4 SV=1)

HSP 1 Score: 2511.9 bits (6509), Expect = 0.0e+00
Identity = 1299/1668 (77.88%), Postives = 1427/1668 (85.55%), Query Frame = 0

Query: 1    MRTRFLDIDYFAAGNEPFRCLPVPHLASKPPLSTAGDLLHFDFLPEISLAIDRLAIDSAL 60
            MRTRFL+ID+FA GNE F  LPVPHL S  PLST GDLLHFDFLPEISL ID L+IDSA+
Sbjct: 1    MRTRFLNIDFFAPGNESFHRLPVPHLVSN-PLSTVGDLLHFDFLPEISLGIDSLSIDSAI 60

Query: 61   SKFFDDVLPRRIDDDNDYRD-----SRFQGSTERI--------RFLEEEAKCAYEDNVEG 120
            S+FFDDVLPRRI DD+ YRD     S F GS ERI        R  EEEAK  YEDN+EG
Sbjct: 61   SRFFDDVLPRRIVDDDVYRDAGCQSSGFHGSAERIFSSESVGTRLREEEAKGTYEDNMEG 120

Query: 121  GWKNFGSETSETEF--KAIGTVIRNRNLTYDMIQFETPQLDAFLENAFLFEREEVQILAE 180
             WKNFGSETSE EF  K IGT IRNRN + DMIQFETPQLD FLENAFLFE+EEVQIL  
Sbjct: 121  SWKNFGSETSEMEFVKKDIGTEIRNRNFSNDMIQFETPQLDTFLENAFLFEKEEVQILTA 180

Query: 181  FPEAKFNLETLNQGLLNCPSEVKESVYAIEGITSEYLLDQKSCLVEEDFAQDQKLLHRLT 240
             PE +F+LETLNQGLL  PSEVKES+Y +E I SEYLLDQ+SCL E+DFAQDQKL H+ T
Sbjct: 181  MPEVEFDLETLNQGLLKYPSEVKESIYDVEIIPSEYLLDQESCLFEDDFAQDQKLSHQFT 240

Query: 241  FPFLEVDEMVLETSAFFSMQDELCFVLDNSESPHSIQDDNLLVNDEENLCSMKSDIEEFL 300
            FPFLEVDEMVLET AF S+QDEL F+L+++ES H I DDNLLVN+EE L SM+ D EEFL
Sbjct: 241  FPFLEVDEMVLETLAFLSLQDELFFILESTESAHMIHDDNLLVNNEEYLSSMRCDAEEFL 300

Query: 301  LDHFLRPCAVSEVASPDISGG-SDLMSLMEILEIPVSSAVQTKPSCDLPVGLAIFEEFQL 360
             DHFLRPC VSE+ASPDISGG SDLMS+ME LEI VSS VQTK SCDL V  AIFEEFQL
Sbjct: 301  SDHFLRPCGVSELASPDISGGSSDLMSMMETLEIAVSSEVQTKSSCDLSVEPAIFEEFQL 360

Query: 361  LDIYSNQPFEAFFDLELSAVSEISDCMSIENMNFKSFNELIVCHELALVDDTFKSLPVPL 420
            LD YSNQ F A FDLE+SA+SEI+DC SIEN NFKSFNELIVCHEL LVDDTFKSLPVP+
Sbjct: 361  LDTYSNQHFGAIFDLEVSAMSEITDCTSIENTNFKSFNELIVCHELVLVDDTFKSLPVPI 420

Query: 421  LSSEESERPMFAFIEDVLANLNGKSLSTSDGIYLDWYLLEEDSCSFGIYSSYQNLLEEIN 480
            LSS+ SE+ + AFIEDVLANLN +SLS SDGIYLDW+LL+E S S G+YSSYQN+LEEIN
Sbjct: 421  LSSQGSEKLLNAFIEDVLANLNKQSLSASDGIYLDWHLLDESSYSSGLYSSYQNMLEEIN 480

Query: 481  LKPVEFDQEPCGYDSIFYRYVFSDDALDRERTEDKGELKEPFPDGISMLPSQTIDVASSK 540
            LKPVEFD+EPC  D IFYRYVFSDDAL RERTEDKGELKE FP+GI ML  QTIDVASS 
Sbjct: 481  LKPVEFDREPCENDGIFYRYVFSDDALVRERTEDKGELKESFPEGIPMLSGQTIDVASSI 540

Query: 541  LLNDGCQQLGRQNLVAVGNTEKVLSSWKSQSELNDPKFFLNSQKPAGTRKGESVFSATDI 600
            LLN+ CQQ  RQ L AVGN+EK LSSWKS+SE ND KFFLNSQKP G RK ESVFS  D 
Sbjct: 541  LLNERCQQKERQGLAAVGNSEKALSSWKSKSESNDLKFFLNSQKPVGVRKSESVFSEIDT 600

Query: 601  NTILPKVPCDGQLTNNPSTTSADVSLKQLNVTLHQVCLSDNILHLINNSEKTYLAILQNE 660
            NT LPKVP DG LTN PS +SAD SLKQLNV +HQVCLSDNILHLINNSEKTYLAILQNE
Sbjct: 601  NTTLPKVPRDGILTNKPSMSSADDSLKQLNVAVHQVCLSDNILHLINNSEKTYLAILQNE 660

Query: 661  TELRKTYLPYVAEDYVLMLSLPKQKLIDCVKKIYLLGTSNYWEEKVMTLATLYAIKQIAW 720
            TELRK YLPYVAEDY+LMLSLPKQKLIDC+KKIYL GT+ YWEEK+MTLATLYA KQI W
Sbjct: 661  TELRKKYLPYVAEDYLLMLSLPKQKLIDCIKKIYLQGTNTYWEEKIMTLATLYATKQIVW 720

Query: 721  YLCFYGIHPAHLYLKKLCQSLECLKSRLGFLASLIEDASKMVDREITISHPALTTIQDIL 780
            YLCFYGIHPAHLYLKKLCQS ECLKSRLGFL S IE+A KMV REITISHPALTTIQ+IL
Sbjct: 721  YLCFYGIHPAHLYLKKLCQSSECLKSRLGFLVSSIEEAGKMVGREITISHPALTTIQEIL 780

Query: 781  CSKTSSSTLKVLVVANEIFWGSLKKLFGSMGLSFEELSFGGPTDQQVFNANSMVDELVPN 840
            CS+TS+S+LKVLVVAN+IFW SL+KL GS+G+SF E+++G  T +QV NAN+MVD+L  N
Sbjct: 781  CSRTSTSSLKVLVVANQIFWWSLEKLLGSLGISFAEINYGSLTVEQVSNANAMVDDLASN 840

Query: 841  CLLVSQGHISGSFPFNKFSIILEYGGINGSSQISTYFTKSINMPHLHFVMLELEKCGNCK 900
            CLLVSQ +ISGSFPFNKFSI+LEYGG+NGSSQIS YF+  I+MPHLHF+MLEL+KCGNCK
Sbjct: 841  CLLVSQEYISGSFPFNKFSIVLEYGGLNGSSQISAYFSNLIDMPHLHFIMLELDKCGNCK 900

Query: 901  AFCKGVGLPQHNELKIEEKSLVENQTRMLEELLNFLPVDEKNILAPPEATIEAEDLKVPL 960
            AFC+GV  PQHNEL IEEKSLVENQT MLE+LLNFLPV+EK ILA P+ TIEAE+ +VPL
Sbjct: 901  AFCEGVDFPQHNELTIEEKSLVENQTMMLEKLLNFLPVEEKYILASPKKTIEAENRRVPL 960

Query: 961  QVSVGPVSGKSQHIDLVSFPEAIVIVNTQKFEKEMIVSRRSSYQRLLALEKDGVQVVERD 1020
            +  V PVS KSQ+ DLVSFPEAI+IVNTQKFEKEMIV RRSSY+R+LALEK+GVQVVERD
Sbjct: 961  RAPVEPVSDKSQYTDLVSFPEAIIIVNTQKFEKEMIVCRRSSYRRILALEKEGVQVVERD 1020

Query: 1021 LSFPVDLIITSGLCLMWYDCTNIHRKASTPNEASSCMNLCIENIATDVLTSLSFAFSGCI 1080
            L  PVDLII  G+CL WYDCTNI RK ST NEAS C+NLCIENIATDVLTSLSFAF GC+
Sbjct: 1021 LCLPVDLIIAPGICLFWYDCTNIDRKGSTSNEASLCLNLCIENIATDVLTSLSFAFRGCV 1080

Query: 1081 LVFEGEISYLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKSMNRH 1140
            LVFEGEIS LSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSK M R 
Sbjct: 1081 LVFEGEISSLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENVSKLMTRR 1140

Query: 1141 LYPKMSESETLAESFLTSFPSINPLTAHGILSSESFLCDFLEWPHERRLHAVKKYCIPDE 1200
            +YPKMSESETLAESFLTSFPSINPL AHGILSSES L DFLEWPHERRLHA++KY IPD+
Sbjct: 1141 IYPKMSESETLAESFLTSFPSINPLIAHGILSSESLLADFLEWPHERRLHAIRKYSIPDD 1200

Query: 1201 SVSLFSVLCKYGEREDSKSVMTDCSSSVSSGPDSDKCYFNGNSKRKRRNFTGGTQCIDKN 1260
             +SLFS LCKYGEREDSKSVMTDCSSS+SSG DS+KC+FNGNS+RKRRNFTGGTQCI+KN
Sbjct: 1201 CISLFSALCKYGEREDSKSVMTDCSSSLSSGADSEKCHFNGNSERKRRNFTGGTQCIEKN 1260

Query: 1261 MDVFNSDAPNPFSAGSRETLSASKSLGSQMFEDQEIFSGLKGLSSSVNHFFDQNHNLESF 1320
             D   S+  NPF+AG+ ETL ASKS GSQ+FED EIF  LKGLSSSVN+FFDQNHNL SF
Sbjct: 1261 TDCLYSNTLNPFTAGTAETLVASKSFGSQLFEDPEIFCDLKGLSSSVNNFFDQNHNLASF 1320

Query: 1321 DASISMNPNSFGKSRDSWISTSPEISNEIRRHCSSYVQNQGLDSNRRKRHNLHNMNKSEN 1380
            DA++SM+P    K RD WIST+PEIS+EIRRHCS +VQNQGLD +++K H+ HNMNKSEN
Sbjct: 1321 DATVSMDPTRVCKPRDCWISTAPEISDEIRRHCSPFVQNQGLDRSKKKIHSFHNMNKSEN 1380

Query: 1381 QHEELTGEVVSLIDDPVFKDDFASMAPMNFLPSMLDYEKDSPRKSKIQRKLYHGQSDHPF 1440
             HEEL  EV +LID+PV KD FA+MAPMNFLPSML+ E DS RKSKIQR+L +GQSDHPF
Sbjct: 1381 HHEELIAEVDNLIDNPVLKDHFATMAPMNFLPSMLENETDSSRKSKIQRRLSYGQSDHPF 1440

Query: 1441 SAVGMANNSSSDFWSPMNFHRQNSGGLDNHFPDPSFQP-----RYKDDHSDEGLMQNSVR 1500
             AV + NNSSSDFWS +N H Q+S GLDN FPDPSF+P     RYKDDH DEGL QN +R
Sbjct: 1441 CAVDVGNNSSSDFWSSINLHGQSSRGLDNQFPDPSFEPSIMPLRYKDDHLDEGLTQNHLR 1500

Query: 1501 DSKVLFSLSEKGMSHSDVTPLSIALRSKHLQESSPWTMEFLNRIKEKSRNRLHSVPCGSS 1560
            DSKV FSLSEK  SHSDVTPLS+ALRSKHLQESSPWTMEFLNRI+EKSRNR HSVP GSS
Sbjct: 1501 DSKVSFSLSEKDTSHSDVTPLSVALRSKHLQESSPWTMEFLNRIREKSRNRQHSVPRGSS 1560

Query: 1561 SPFPEHLGNVRKTVKRRSPSILEFFKYQGGSTQRKKPEQKRQKQSMQSSNSSKNVLVPTN 1620
            SPFPE+LGNV+KTVKRRSPSILEFFKYQGGST RKKPEQKR K S QSSNSSKNVLV T 
Sbjct: 1561 SPFPENLGNVKKTVKRRSPSILEFFKYQGGSTLRKKPEQKRHKPSTQSSNSSKNVLVATR 1620

Query: 1621 ELSSWTPIDKRSRQTLSFAIDGNERQTKLVWSNDNYGLGQSSQKLTNK 1648
            ELSS TPIDKRSRQTLS+A DGN  QTKLVWSNDNYGLG++SQKL NK
Sbjct: 1621 ELSSGTPIDKRSRQTLSYATDGNGSQTKLVWSNDNYGLGKNSQKLGNK 1667

BLAST of Sed0022815 vs. TAIR 10
Match: AT5G52290.1 (shortage in chiasmata 1 )

HSP 1 Score: 784.3 bits (2024), Expect = 2.0e-226
Identity = 608/1682 (36.15%), Postives = 900/1682 (53.51%), Query Frame = 0

Query: 1    MRTRFLDIDYFAAGNE------PFRCLPVPHLASKPPL-STAGDLLHFDFLPEISLAIDR 60
            MRTRFL+IDYF+           F  LP P     P + +   D L F  +  +S+ I  
Sbjct: 1    MRTRFLNIDYFSTPPSHVFETLGFLNLPAPDNFPAPIVYNGEEDRLRFGSIENVSIPIGN 60

Query: 61   LAIDSALSKFFDDVLPRRIDDDNDYRDSRFQGSTERIRFLEEEAKCAYEDNVEGGWKNFG 120
            L I++ALSKF  DV+P R+    DYR      S+  + + +E                  
Sbjct: 61   LPIEAALSKFLSDVVPDRV--SVDYRVFEIDDSSLGVYYSDE------------------ 120

Query: 121  SETSETEFKAIGTVIRNRNLTYDMIQFETPQLDAFLENAFLFEREE-VQILAEFPEAKFN 180
                    K  G  I ++  T  +I+ ETP+LD  +EN  L   E+ +Q  +E  E K N
Sbjct: 121  --------KDDGDAIADK-ATPKIIELETPELDFEMENKLLCTSEDHLQCFSEVLEIK-N 180

Query: 181  LETLNQG---LLNCPSEVKESVYAIEGITSEYLLDQKSCLVE-EDFAQDQKLLHRLTFPF 240
                 +G   +L    +++E +Y+++ I S+Y  +  + + E E F + Q       FP 
Sbjct: 181  DPVKYEGSDIILQNSKDIQEQIYSVDYIPSDYFTENNTSVAENECFRKIQPWFKDARFPL 240

Query: 241  LEVDEMVLETSAFFSMQDELCFVLDNSESPHSIQDDNLLVNDEENLCSMKSDIEEFL-LD 300
            LEVDE+ L   +  S+ D++  VL+  E   +    +L++N +E + S   D+ + L  D
Sbjct: 241  LEVDEVNLSELSSLSVLDKVFTVLETIEPQDTNAGSSLIINSKELIGSKDYDLLDVLSTD 300

Query: 301  HFLRPCAVSEVASPDISGGSDLMSLMEILEIPVSSAVQTKPSCDLPVGLAIFEEFQLLDI 360
             +L     S+V   D     D+++++EI     S+A + +    +PV    +EEFQ+LD+
Sbjct: 301  CYLNKSGQSDVVPEDEFSEMDIVTILEI-----SNAEEFQGKVAVPV---TYEEFQILDV 360

Query: 361  YSNQPFEAFFDLELSAVSEISDCMSIENMNFKSFNELIVCHELALVDDTFKSLPVPLLSS 420
              +  F+ F  L+ +   EI   M  + MNFK F+EL+V  ELA  DD FKSLP P+L  
Sbjct: 361  DISDVFDIFLCLQKAIEPEICYGMFSKEMNFKDFDELVVSSELAFTDDAFKSLPTPILHD 420

Query: 421  EESERPMFAFIEDVLANLNGKSLSTSDGIYLDWYLLEEDSCSFGIYSSYQNLLEEINLKP 480
             E  R +    EDVL+ +  +SLS S+ IYL W LLEE + +   Y       EEI    
Sbjct: 421  YEMTRSLELIYEDVLSKIKPQSLSASNDIYLPWNLLEERNHNHCDYP-----FEEIVTFN 480

Query: 481  VEFDQEPCGYDSIFYRYVFSDDALDRERTEDKGELKEPFPDGISMLPSQTIDVASSKLLN 540
            ++++ E    D   Y ++FS+DA      E   +  EPF  GIS L        S  LL 
Sbjct: 481  IDYNWEASEGDKWVYDFIFSEDAFCEPLVE---KCTEPF-YGISNLDEHAPVNTSHGLLE 540

Query: 541  DGCQQLGRQNLVAVGNTEKVLSSWKSQSELNDPKFFLNSQKPAGTRKGES-VFSATDIN- 600
            +  Q+ G ++     N +K    +KS S  +D  FF++ +K       ES V +A   N 
Sbjct: 541  NPFQKTGARDCAVDDNAKKATLLFKSMSAFDDLTFFMDPKKAVIEDNLESRVEAAKTTNH 600

Query: 601  ---TILPKVPC-DGQLTNNPSTTSADVSLKQLNVTLHQVCLSDNILHLINNSEKTYLAIL 660
               +I  K  C  G +  NP T           + LH V  S+NI  L+    K+YL ++
Sbjct: 601  KCMSIDSKASCRSGGMHPNPKTE---------EMILHSVRPSENIQALVGEFVKSYLTLV 660

Query: 661  QNETELRKTYLPYVAEDYVLMLSLPKQKLIDCVKKIYLLGTSNYWEEKVMTLATLYAIKQ 720
            ++E+E        ++ED + +LS+ K KLIDC++K  +  T    ++K  T A L AIKQ
Sbjct: 661  KDESE-------NLSEDKLKLLSISKGKLIDCIRKANVHKT-QLADDKTFTFALLLAIKQ 720

Query: 721  IAWYLCFYGIHPAHLYLKKLCQSLECLKSRLGFLASLIEDASKMVDREITISHPALTTIQ 780
            + WY+CF+GIH A++YL K+C+S   +K  L  L S +E   K  + +IT SHP+L  IQ
Sbjct: 721  MTWYMCFFGIHVAYIYLNKVCRSSNPMKIGLHTLYSAVETEHKSDETDITRSHPSLAVIQ 780

Query: 781  DILCSKTSSSTLKVLVVANEIFWGSLKKLFGSMGLSFEELSFGGPTDQQ--VFNANSMVD 840
             IL S+ +    K L++A ++FW SLK+L  SMGLS+ +L+   P+  +  V  A  +  
Sbjct: 781  GILQSEFARGNSKALLLAEKVFWSSLKRLLMSMGLSYNDLNSPSPSGNRPNVHEAIELGF 840

Query: 841  ELVPNCLLVSQGHISGSFPFNKFSIILEYGGINGSSQISTYFTKSINMPHLHFVMLELEK 900
              + +CL++S   IS SFP   FS+I+EYGG N S + S + +K  + P  HF+ +EL+ 
Sbjct: 841  LPISDCLIISYEQISPSFPVENFSVIVEYGGPNASPRYS-FPSKLDSFPSFHFIKVELDM 900

Query: 901  CGNCKAFCKGVGLPQHNELKIEEKSLVENQTRMLEELLNFLPVDEKNILAPPEATIEAED 960
               C   C GV +P    LK+ +   VE +T  LEE+LNF+P+++       E T E+E 
Sbjct: 901  PSACGQLCAGVTVPY--SLKMIKGDEVETKTGWLEEVLNFVPLEKVCYAGSSETTNESEF 960

Query: 961  LKVPLQVSVGPVSGKSQHI--DLVSFPEAIVIVNTQKFEKEMIVSRRSSYQRLLALEKDG 1020
            + +P +      S + + I    +S   ++++VNT+  +KEMI+SRRS+YQ++LA+EK+G
Sbjct: 961  ISMPQE------SERKRGIIEQGLSDQRSVIVVNTKTVDKEMIISRRSTYQKVLAMEKEG 1020

Query: 1021 VQVVERDLSFPVDLIITSGLCLMWYDCTNIHRKASTPNEASSCMNLCIENIATDVLTSLS 1080
            VQVVERD   PVDL+++  +CL+WYD   + +K++     SS     I +IAT+VLTSLS
Sbjct: 1021 VQVVERDSDLPVDLMLSPAVCLLWYDSETVSKKSAATIGTSSSSLSWIGDIATNVLTSLS 1080

Query: 1081 FAFSGCILVFEGEISYLSIVMESSDGLYAAAASLEIDFQLFCSYSSELTDEIILGCIENV 1140
            F+FS CI+VFEGE ++L+ VM+SSD LYAAA SL I  Q+FCS S+ LTDEIIL CI++ 
Sbjct: 1081 FSFSTCIMVFEGEPAFLAAVMDSSDELYAAAGSLGISLQMFCSSSANLTDEIILKCIKSS 1140

Query: 1141 SKSMNRHLYPKMSESETLAESFLTSFPSINPLTAHGILSSESFLCDFLEWPHERRLHAVK 1200
             K    H+  KM ESE+LAESFLT FPS+NPLTA  ILSS   L +F++ PH+ ++   +
Sbjct: 1141 VKLSKLHV--KMPESESLAESFLTKFPSVNPLTAQVILSSSGSLLEFMKLPHKSKVERTQ 1200

Query: 1201 KYCIPDESVSLFSVLCKYGEREDSKSVMTDCSSSVSSGPDSDKCYFNGNSKRKRRNFTGG 1260
            KY +P+ESV LFS +C+YG REDS+SVMTD SSSVSSGPDSD  + + +S  K++ +   
Sbjct: 1201 KYHVPEESVDLFSSVCRYGAREDSRSVMTDSSSSVSSGPDSDTHHVSVHSGSKKKQYIAE 1260

Query: 1261 TQCIDKNMDVFNSDAPNPFSAGSRETLSASKSL-GSQMFEDQEI--------FSGLKGLS 1320
               ID  MD     +P+   A ++   S    L  S   +D EI        FS      
Sbjct: 1261 KDEID--MDDLVHFSPSIEFADTQLKSSGDFQLDDSWSSKDHEIFHFDPVTEFSDAPFKP 1320

Query: 1321 SSVNHFFDQ--NHNLESFDASISMNPNSFGKSRDSWISTSPEISNEIR-------RHCSS 1380
            S ++H  D   + + E FD      P S  K    W    P+ S E            S 
Sbjct: 1321 SGISHPNDSWPSKDPERFDK--KSGPGSSSKD-TFWEKDQPDFSVEDSLPGIPELEDWSF 1380

Query: 1381 YVQNQGLDSNR------RKRHNLHNMNKSENQHEELTGEVVSLIDDPVFKDDFASMAPMN 1440
             V+++ +  NR       +  NLH+   SEN   +  GEV+   D  + +D   S     
Sbjct: 1381 PVKDKFMSQNRGCKFPVMRDFNLHDNRNSENFIADYKGEVIDRADKYLEEDFPPSPGYNR 1440

Query: 1441 FLPSMLDY-EKDSPRKSKIQRKL-YHGQSDHPFSAVGMANNSSSDFWSPMNFHRQNSGGL 1500
            F   + D  E++ PRKSK  RKL + G     F      ++SS  + +  +    N+  L
Sbjct: 1441 FARIVSDVNEEELPRKSKSSRKLSFFGSLQPNFPKAADIDSSSERYATEKDSKYDNNTSL 1500

Query: 1501 DNHFPDPSFQPRYKDDHSDEGLMQNSVRDSKVLFSLSEKGMSHSDVTPLSIALRSKHLQE 1560
              +    ++  + +    +E L + S   +  L    E  +SH   +PLS A+RS +  +
Sbjct: 1501 RGY--ADNYPAKRQRTLLEEVLTRRSAVPTTELPFREE--ISHFGGSPLSNAIRSSNQVQ 1560

Query: 1561 SSPWTMEFLNRIKEKSRNR--LHSVPCGSSSPFPEHLGNVRK-TVKRRSPSILEFFKYQG 1620
            SSPWT++FLNR++E+SR R    S+P  +S P  E  GN++K   KR+SPSILEFFKY+G
Sbjct: 1561 SSPWTVDFLNRVRERSRARKQQQSLPSYASPPSLETPGNIKKANTKRKSPSILEFFKYKG 1593

Query: 1621 GSTQRKKPEQKRQKQSMQSSNSSKNVLVPTNELSSWTPIDKRSRQTLSFAIDGNERQTKL 1631
            G+   K  E+KRQK+S  SS S KN     + L S TPIDKR++Q+LS+  +G   QTKL
Sbjct: 1621 GN---KLQEEKRQKRSKNSSASPKNERF-YSPLKSCTPIDKRAKQSLSYTANGT-GQTKL 1593