Sed0022645 (gene) Chayote v1

BLAST of Sed0022645 vs. NCBI nr
Match: XP_022142008.1 (probable ubiquitin-conjugating enzyme E2 24 [Momordica charantia] >XP_022142009.1 probable ubiquitin-conjugating enzyme E2 24 [Momordica charantia] >XP_022142010.1 probable ubiquitin-conjugating enzyme E2 24 [Momordica charantia])

HSP 1 Score: 1588.9 bits (4113), Expect = 0.0e+00
Identity = 780/912 (85.53%), Postives = 825/912 (90.46%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLVEAEY-YHGQTSCILSSLEETIGKIDEFLSFEREFMLG 60
           MDLFLSDSDWENSSECDSSDD  EAEY YHGQTSCILSSLEETIGKIDEFLSFEREFMLG
Sbjct: 1   MDLFLSDSDWENSSECDSSDDPEEAEYFYHGQTSCILSSLEETIGKIDEFLSFEREFMLG 60

Query: 61  DVVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISVGDAVVHGPWIG 120
           DVVCSVNDPFGQMGKVVGIDMLVDLEKNHGP IKDVNSKDLLKIRSISVGD VVHG WIG
Sbjct: 61  DVVCSVNDPFGQMGKVVGIDMLVDLEKNHGPFIKDVNSKDLLKIRSISVGDVVVHGSWIG 120

Query: 121 RVDKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVPKPPR 180
           RVDKVIDSVSVLFDDGTKCEIIAVDQEKLL ISVDALDD QYPYYPGQRVR SNV K PR
Sbjct: 121 RVDKVIDSVSVLFDDGTKCEIIAVDQEKLLSISVDALDDPQYPYYPGQRVRLSNVSKSPR 180

Query: 181 WLSGWKDNQCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFPHAN 240
           WLSGWKD++CEGTVCSVEAGLVYVDWLASVLMGCSSSL AP RMQHAK+LTLLSCFPHAN
Sbjct: 181 WLSGWKDDRCEGTVCSVEAGLVYVDWLASVLMGCSSSLPAPQRMQHAKKLTLLSCFPHAN 240

Query: 241 WHLGDWCIFPEDEKNDYLGEKFIS--------------NEISVIVKKKTKVDVLWQDGSS 300
           WHLGDWCIFP DEK DYLGEKF +               EIS+IVKKKTKVDVLWQDGS 
Sbjct: 241 WHLGDWCIFPVDEKGDYLGEKFTTTHDLMNGGYCGTGFQEISIIVKKKTKVDVLWQDGSR 300

Query: 301 STGLDSHCLTPANAMNAHEFWPQQLVMDEGHCDDPSMSITQRWGVVQAVDAKERTVKVQW 360
           S+ LDSHCL PANAMNAHEFWPQQLVM++G C+DPS+SI +RWGVV+AVDAKERTVKVQW
Sbjct: 301 SSELDSHCLAPANAMNAHEFWPQQLVMEKGTCNDPSISIAERWGVVRAVDAKERTVKVQW 360

Query: 361 KMGDLTRGTDLDGPLVEEIMSAYELAEHPDYSFSLGDIVFRLALDKFGGQSDESNINDEI 420
           +  DLT G DLD  LVEE MSAYEL EHPDYSFSLGDIVFRLALDKFGGQSDE N N EI
Sbjct: 361 RTSDLTEGNDLDILLVEETMSAYELVEHPDYSFSLGDIVFRLALDKFGGQSDEHNTNYEI 420

Query: 421 SANEESAQKGENRVIDNNMDMDNFISFFGNVTGFKDGAVEVRWANCSTTMVPPQEIFPID 480
           S +EESAQ GENRVIDN M+MDNF+SF GNV GFKDG +EVRWANCST+ V PQEIFPID
Sbjct: 421 SMSEESAQNGENRVIDNTMNMDNFLSFIGNVIGFKDGVMEVRWANCSTSKVLPQEIFPID 480

Query: 481 KYEVSTISVPDGENVEELRQDQIEPLKPYNIQKGKDVPNCDGVSKNFKRDTTCFFSRAAI 540
           KYEVST++VP+GENVEELRQDQIE LKPYNIQKGKD+PNCDG  KNFKRDTTCFFSRAAI
Sbjct: 481 KYEVSTLAVPNGENVEELRQDQIEHLKPYNIQKGKDMPNCDGAGKNFKRDTTCFFSRAAI 540

Query: 541 DFFSNVFTTLFGSVRSTILSGTFTALQSFEDRNEIGLLHEREVLQTCSQSNEMSQIAKLQ 600
           +FFS++FTTLFGSVRSTILSGTFTALQSFE  +EIGLL+E+E LQTC QS E+SQIAKLQ
Sbjct: 541 EFFSSIFTTLFGSVRSTILSGTFTALQSFEGGDEIGLLNEKEELQTCCQSTELSQIAKLQ 600

Query: 601 SFGHTNMSIEEIHKNEDLTLSASNENYDLFTQFDMTSDCSDHHFLSSGKGSVLYQTRRGW 660
            FG T+  IEE HKNED TLS S+ENYDLFTQFDM SDC DHHFLSSG GS+L Q RRGW
Sbjct: 601 DFGETSFKIEENHKNEDFTLSTSSENYDLFTQFDMASDCPDHHFLSSGNGSLLSQARRGW 660

Query: 661 LKKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQ 720
           LKKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQ
Sbjct: 661 LKKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQ 720

Query: 721 VPPLVHYISGGLRINPNLYESGKVCLSLLNTWTGSGTEVWNAGSSTILQVLLSLQALVLN 780
           +PPLVHYISGGLR+NPNLYESGKVCLSLLNTWTGSG+EVWN  SSTILQVLLSLQALVLN
Sbjct: 721 IPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPVSSTILQVLLSLQALVLN 780

Query: 781 EKPYYNEAGYDAQLGRAEGERNSVSYNENAFLITCQSMMYLLTKPPKHFEALVEEHFRRR 840
           EKPYYNEAGYDAQLGRAEGE+NS SYNENAFL+TC+SMMYLL+KPPKHFEALVEEHFRRR
Sbjct: 781 EKPYYNEAGYDAQLGRAEGEKNSDSYNENAFLVTCKSMMYLLSKPPKHFEALVEEHFRRR 840

Query: 841 YQHILAACRAYMEGTPVGSDYHTGAAELENSNQCSTGFKIMLAKIFPRLIKTFSSKGIDC 898
           YQHILAAC AYMEGTPVGSDYH GA +LE  NQ STGFKIMLAKIFP+L++TFSSKGIDC
Sbjct: 841 YQHILAACIAYMEGTPVGSDYHCGATKLEYPNQSSTGFKIMLAKIFPKLVETFSSKGIDC 900

BLAST of Sed0022645 vs. NCBI nr
Match: XP_038881614.1 (probable ubiquitin-conjugating enzyme E2 24 [Benincasa hispida])

HSP 1 Score: 1577.4 bits (4083), Expect = 0.0e+00
Identity = 767/911 (84.19%), Postives = 825/911 (90.56%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLVEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60
           MDLFLSDSDWENSSECDSSDDL EAEYYHGQTSCILSSLEETIGKIDEFLSFEREF+LGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  VVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISVGDAVVHGPWIGR 120
            VCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSK+LLKIRSIS GD VV+GPWIGR
Sbjct: 61  AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKNLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVPKPPRW 180
           VDKVIDSVSVLFDDGTKCEI  VDQEKLLPISVDALDDSQYPYYPGQRVRFSNV KPP W
Sbjct: 121 VDKVIDSVSVLFDDGTKCEITTVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDNQCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFPHANW 240
           LSGWKD++CEGTVC VEAGLVYVDWLASVLMGCSSSL APPR+QHAKEL  LSCFPHANW
Sbjct: 181 LSGWKDDRCEGTVCYVEAGLVYVDWLASVLMGCSSSLMAPPRLQHAKELISLSCFPHANW 240

Query: 241 HLGDWCIFPEDEKNDYLGEKFIS--------------NEISVIVKKKTKVDVLWQDGSSS 300
           +LGDWCIFP DEK++Y GEK I+               EI VIVKKKTKVDVLWQDGS S
Sbjct: 241 NLGDWCIFPVDEKDNYFGEKLITTHDLINGGYRGTGFQEICVIVKKKTKVDVLWQDGSRS 300

Query: 301 TGLDSHCLTPANAMNAHEFWPQQLVMDEGHCDDPSMSITQRWGVVQAVDAKERTVKVQWK 360
           TGLDSHCLTPAN MNAHEFWPQQ VM++G+CDD SMS+ QRWGVVQAVDAKERTVKVQ +
Sbjct: 301 TGLDSHCLTPANVMNAHEFWPQQFVMEKGNCDDSSMSVYQRWGVVQAVDAKERTVKVQMR 360

Query: 361 MGDLTRGTDLDGPLVEEIMSAYELAEHPDYSFSLGDIVFRLALDKFGGQSDESNINDEIS 420
             DL+ G++LDG LVEE MSAYEL EHPDY+FSLGD+VFRLALDKFG QSD++NI  EIS
Sbjct: 361 TDDLSEGSNLDGLLVEETMSAYELVEHPDYNFSLGDVVFRLALDKFGSQSDKNNIISEIS 420

Query: 421 ANEESAQKGENRVIDNNMDMDNFISFFGNVTGFKDGAVEVRWANCSTTMVPPQEIFPIDK 480
            N ESAQ G+N ++DNNM+MDNF+SF G+VTGFKDGAVEV+WANCST+MV PQEIFPIDK
Sbjct: 421 MN-ESAQNGKNHIVDNNMNMDNFLSFIGHVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480

Query: 481 YEVSTISVPDGENVEELRQDQIEPLKPYNIQKGKDVPNCDGVSKNFKRDTTCFFSRAAID 540
           YE+ST + P+GEN+EE RQDQIEPLKPYNIQKGKD+PNCDG  KNFKRDTTCFFSRAAI+
Sbjct: 481 YEISTTAEPNGENLEESRQDQIEPLKPYNIQKGKDMPNCDGAGKNFKRDTTCFFSRAAIE 540

Query: 541 FFSNVFTTLFGSVRSTILSGTFTALQSFEDRNEIGLLHEREVLQTCSQSNEMSQIAKLQS 600
           FFSN+F+T FGSVRSTI+SGTFTALQSFE  +EI LL E+EVLQTCS+S EMSQIAK  +
Sbjct: 541 FFSNIFSTFFGSVRSTIISGTFTALQSFEGEDEIDLLCEKEVLQTCSESCEMSQIAKSHN 600

Query: 601 FGHTNMSIEEIHKNEDLTLSASNENYDLFTQFDMTSDCSDHHFLSSGKGSVLYQTRRGWL 660
           FG T+ +IEEIHK EDLTLS S+ENYDLF QFDM SDC DHHFLSSGKGS+L Q  RGWL
Sbjct: 601 FGETS-NIEEIHKYEDLTLSTSSENYDLFAQFDMASDCPDHHFLSSGKGSILSQASRGWL 660

Query: 661 KKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQV 720
           KKVQQEWSILKNNLPET+YVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQ+
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQI 720

Query: 721 PPLVHYISGGLRINPNLYESGKVCLSLLNTWTGSGTEVWNAGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLR+NPNLYESGKVCLSLLNTWTGSG+EVW+ GSSTILQ+LLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWSPGSSTILQILLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSVSYNENAFLITCQSMMYLLTKPPKHFEALVEEHFRRRY 840
           KPYYNEAGYDAQLGR EGERNS SYNENAFL+TC+SMMYLL+KPPKHFE LVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRTEGERNSASYNENAFLVTCKSMMYLLSKPPKHFETLVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYHTGAAELENSNQCSTGFKIMLAKIFPRLIKTFSSKGIDCS 898
           QHILAACRAYMEGTPVGSDYH G  +LEN NQ STGFKIMLAKIFPRLIKTFSSKGIDCS
Sbjct: 841 QHILAACRAYMEGTPVGSDYHRGTTKLENPNQSSTGFKIMLAKIFPRLIKTFSSKGIDCS 900

BLAST of Sed0022645 vs. NCBI nr
Match: XP_022978639.1 (probable ubiquitin-conjugating enzyme E2 24 [Cucurbita maxima] >XP_022978640.1 probable ubiquitin-conjugating enzyme E2 24 [Cucurbita maxima] >XP_022978641.1 probable ubiquitin-conjugating enzyme E2 24 [Cucurbita maxima])

HSP 1 Score: 1566.6 bits (4055), Expect = 0.0e+00
Identity = 764/902 (84.70%), Postives = 815/902 (90.35%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLVEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60
           MDL LSDSDWENSS+CDSSDDL EAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD
Sbjct: 1   MDLILSDSDWENSSDCDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60

Query: 61  VVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISVGDAVVHGPWIGR 120
           VVCSVNDPFGQMGKVVGIDMLVDLE NHGP+IKDVNSKDL KIR ISVGD VVHG WIGR
Sbjct: 61  VVCSVNDPFGQMGKVVGIDMLVDLETNHGPIIKDVNSKDLRKIRPISVGDVVVHGKWIGR 120

Query: 121 VDKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVPKPPRW 180
           V+KVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSN  KPPRW
Sbjct: 121 VEKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNASKPPRW 180

Query: 181 LSGWKDNQCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFPHANW 240
           LSGWKDN+CEGTVCSV+AGLVYVDWLAS+LMGCSSSLTAPPRMQHAKELT+LSCFPHANW
Sbjct: 181 LSGWKDNRCEGTVCSVDAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTVLSCFPHANW 240

Query: 241 HLGDWCIFPEDEKNDYLGEKFIS--------------NEISVIVKKKTKVDVLWQDGSSS 300
           HLGDWCIFP DEK+DYLGEKFI+               EISVIVKKKTKVDVLWQDGS S
Sbjct: 241 HLGDWCIFPVDEKSDYLGEKFITAHDLLTGGYRDTGLQEISVIVKKKTKVDVLWQDGSHS 300

Query: 301 TGLDSHCLTPANAMNAHEFWPQQLVMDEGHCDDPSMSITQRWGVVQAVDAKERTVKVQWK 360
            GLDSHCLTPANAMNAHEFWPQQ VM++G+CDD SMS TQRWGVVQAVDAKERTVKVQ +
Sbjct: 301 RGLDSHCLTPANAMNAHEFWPQQFVMEKGNCDDSSMSETQRWGVVQAVDAKERTVKVQLR 360

Query: 361 MGDLTRGTDLDGPLVEEIMSAYELAEHPDYSFSLGDIVFRLALDKFGGQSDESNINDEIS 420
            GDLT G+DLDG  VEE MSAYEL EHPDYSF+LGDIVFRLA   FG QSDE+NI  EIS
Sbjct: 361 TGDLTEGSDLDGLWVEETMSAYELVEHPDYSFTLGDIVFRLA--SFGSQSDENNITSEIS 420

Query: 421 ANEESAQKGENRVIDNNMDMDNFISFFGNVTGFKDGAVEVRWANCSTTMVPPQEIFPIDK 480
            NEESAQ GE+ +I NNM+MDNF+SF GNVT FKDG VEV+WANCS++MV P EIFPIDK
Sbjct: 421 MNEESAQNGESHIISNNMNMDNFLSFIGNVTSFKDGTVEVKWANCSSSMVSPHEIFPIDK 480

Query: 481 YEVSTISVPDGENVEELRQDQIEPLKPYNIQKGKDVPNCDGVSKNFKRDTTCFFSRAAID 540
           YEVS I+VP G+NVEELRQDQIEP KP NIQKGKD+PN D   KNF+RDTTCF SRAAI+
Sbjct: 481 YEVSAIAVPSGDNVEELRQDQIEPSKPDNIQKGKDMPNFDAAGKNFRRDTTCFLSRAAIE 540

Query: 541 FFSNVFTTLFGSVRSTILSGTFTALQSFEDRNEIGLLHEREVLQTCSQSNEMSQIAKLQS 600
           FFSNVFTTLFGSVRSTI+SGTFTALQSFE  + I +L E+EVLQTCSQ    SQIAKL+S
Sbjct: 541 FFSNVFTTLFGSVRSTIISGTFTALQSFEGGDNIDILPEKEVLQTCSQFTGRSQIAKLKS 600

Query: 601 FGHTNMSIEEIHKNEDLTLSASNENYDLFTQFDMTSDCSDHHFLSSGKGSVLYQTRRGWL 660
           FG T+++ EEIH+NE  TLS S+ENYDLFTQFDM SDC DHHFLSSG  S+L Q RRGWL
Sbjct: 601 FGETSLNTEEIHENEASTLSTSSENYDLFTQFDMASDCPDHHFLSSGNASMLSQARRGWL 660

Query: 661 KKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQV 720
           KKVQQEWSILKNNLPET+YVRVFEERMDLIR+VIIGAPGTPYHDGLFFFDIFLPPEYPQ+
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRSVIIGAPGTPYHDGLFFFDIFLPPEYPQI 720

Query: 721 PPLVHYISGGLRINPNLYESGKVCLSLLNTWTGSGTEVWNAGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLR+NPNLYESGKVCLSLLNTWTGSG+EVWN GSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSVSYNENAFLITCQSMMYLLTKPPKHFEALVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNS SYNENAFL+TC+SMMYLL+KPPKHFEALVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEALVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYHTGAAELENSNQCSTGFKIMLAKIFPRLIKTFSSKGIDCS 889
           QHILAACRAYMEGTPVG+DYH+GA +L N NQ STGFKIMLAKI+PRLIKTFSSKGIDCS
Sbjct: 841 QHILAACRAYMEGTPVGADYHSGAKKLVNPNQSSTGFKIMLAKIYPRLIKTFSSKGIDCS 900

BLAST of Sed0022645 vs. NCBI nr
Match: XP_008439854.1 (PREDICTED: probable ubiquitin-conjugating enzyme E2 24 [Cucumis melo] >XP_008439855.1 PREDICTED: probable ubiquitin-conjugating enzyme E2 24 [Cucumis melo] >XP_008439856.1 PREDICTED: probable ubiquitin-conjugating enzyme E2 24 [Cucumis melo] >XP_008439858.1 PREDICTED: probable ubiquitin-conjugating enzyme E2 24 [Cucumis melo] >KAA0052687.1 putative ubiquitin-conjugating enzyme E2 24 [Cucumis melo var. makuwa] >TYK13137.1 putative ubiquitin-conjugating enzyme E2 24 [Cucumis melo var. makuwa])

HSP 1 Score: 1565.1 bits (4051), Expect = 0.0e+00
Identity = 762/911 (83.64%), Postives = 819/911 (89.90%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLVEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60
           MDLFLSDSDWENSSECDSSDDL EAEYYHGQTSCILSSLEETIGKIDEFLSFEREF+LGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  VVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISVGDAVVHGPWIGR 120
            VC VNDP+GQMGKVVGI+MLVDLEKN GPLIK+VNSKDLLKIRSIS GD VV+GPWIGR
Sbjct: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVPKPPRW 180
           VDKVIDSVSVLFDDG KCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNV KPP W
Sbjct: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDNQCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFPHANW 240
           LSGWKD++CEGTVCSVEAGLVYVDWLAS+LMGCSSSLTAPPRMQHAKELTLLSCF HANW
Sbjct: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTLLSCFSHANW 240

Query: 241 HLGDWCIFPEDEKNDYLGEKFISN--------------EISVIVKKKTKVDVLWQDGSSS 300
           +LGDWCIFP DEKN+Y GEKF S               EI +IVKKKTKVDVLWQDGS S
Sbjct: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300

Query: 301 TGLDSHCLTPANAMNAHEFWPQQLVMDEGHCDDPSMSITQRWGVVQAVDAKERTVKVQWK 360
           T LDS CLTPANA NAHEFWPQQ VM++G+CDD SMS+ QRWGVVQAVDAKERTVKVQ +
Sbjct: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 360

Query: 361 MGDLTRGTDLDGPLVEEIMSAYELAEHPDYSFSLGDIVFRLALDKFGGQSDESNINDEIS 420
             DL+ G+ LDG LVEE MSAYEL EHPDYSFSLGDIVFRLA+DKF  QSDE+NI  EIS
Sbjct: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420

Query: 421 ANEESAQKGENRVIDNNMDMDNFISFFGNVTGFKDGAVEVRWANCSTTMVPPQEIFPIDK 480
            NE +  +G + + +NNM+MDNF+SF GNVTGFKDGAVEV+WANCST+MV PQEIFPIDK
Sbjct: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480

Query: 481 YEVSTISVPDGENVEELRQDQIEPLKPYNIQKGKDVPNCDGVSKNFKRDTTCFFSRAAID 540
           YEVS+I+VP+GEN+EE R +QIEPLKPYNIQKGKD+PNCDG SKNFKRDTTCFFSRAA++
Sbjct: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540

Query: 541 FFSNVFTTLFGSVRSTILSGTFTALQSFEDRNEIGLLHEREVLQTCSQSNEMSQIAKLQS 600
           FFSN+FTTLFGSVRSTILSGTFTALQSFE  +EI LL E+EVLQTCSQS EMSQIAKL +
Sbjct: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600

Query: 601 FGHTNMSIEEIHKNEDLTLSASNENYDLFTQFDMTSDCSDHHFLSSGKGSVLYQTRRGWL 660
           FG T++ IEEIHKNEDLT S S++NYDL TQFDM SDC DHHFLSSGKGS+L Q  R WL
Sbjct: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660

Query: 661 KKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQV 720
           KKVQQEWSILKNNLPET+YVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQ+
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720

Query: 721 PPLVHYISGGLRINPNLYESGKVCLSLLNTWTGSGTEVWNAGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLR+NPNLYESGKVCLSLLNTWTGSG+EVWN GSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSVSYNENAFLITCQSMMYLLTKPPKHFEALVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNS SYNENAFL+TC+SMMYLL+K PKHFE LVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYHTGAAELENSNQCSTGFKIMLAKIFPRLIKTFSSKGIDCS 898
           QHILAACRAYMEGTPVGSDY  G  + EN NQ STGFKIMLAKIFPRLIKTFSSKG DCS
Sbjct: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900

BLAST of Sed0022645 vs. NCBI nr
Match: XP_004134704.1 (probable ubiquitin-conjugating enzyme E2 24 [Cucumis sativus] >XP_031743225.1 probable ubiquitin-conjugating enzyme E2 24 [Cucumis sativus] >XP_031743226.1 probable ubiquitin-conjugating enzyme E2 24 [Cucumis sativus])

HSP 1 Score: 1563.1 bits (4046), Expect = 0.0e+00
Identity = 766/911 (84.08%), Postives = 819/911 (89.90%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLVEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60
           MDLFLSDSDWENSSECDSSDDL EAEYYHGQTSCILSSLEETIGKIDEFLSFEREF+LGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  VVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISVGDAVVHGPWIGR 120
            VC VNDP+GQMGKVVGIDMLVDLEKN GP+IK+VNSKDLLKIRSIS GD VV+GPWIGR
Sbjct: 61  AVCFVNDPYGQMGKVVGIDMLVDLEKNSGPIIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVPKPPRW 180
           VDKVIDSVSVLFDDG KCEIIAVDQEKLLPISVD LDDSQYPYYPGQRVRFSNV KPP W
Sbjct: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDVLDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDNQCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFPHANW 240
           LSGWKD++CEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCF HANW
Sbjct: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFSHANW 240

Query: 241 HLGDWCIFPEDEKNDYLGEKFIS--------------NEISVIVKKKTKVDVLWQDGSSS 300
           +LGDWCIFP DEKN+Y GEKFI+               +I VIVKKKTKVDVLWQDGS S
Sbjct: 241 NLGDWCIFPGDEKNNYFGEKFITTHDLMNGGYRGTGFQDICVIVKKKTKVDVLWQDGSRS 300

Query: 301 TGLDSHCLTPANAMNAHEFWPQQLVMDEGHCDDPSMSITQRWGVVQAVDAKERTVKVQWK 360
           T LDS CLTPANA NAHEFWPQQ VM++G+CDD SMS+ QRWGVVQAVDAKERTVKVQ +
Sbjct: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 360

Query: 361 MGDLTRGTDLDGPLVEEIMSAYELAEHPDYSFSLGDIVFRLALDKFGGQSDESNINDEIS 420
             DL+ G++LDG LVEE MSAYEL EHPDYSFSLGDIVFRLALDKF  QSDE+NI  EIS
Sbjct: 361 TDDLSEGSNLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLALDKFASQSDENNIISEIS 420

Query: 421 ANEESAQKGENRVIDNNMDMDNFISFFGNVTGFKDGAVEVRWANCSTTMVPPQEIFPIDK 480
            N ESAQ   NR+I+NNM MDNF+SF GNVTGFKDGAVEV+WANCS +MV PQEIF IDK
Sbjct: 421 MN-ESAQNEGNRIINNNMIMDNFLSFIGNVTGFKDGAVEVKWANCSISMVSPQEIFSIDK 480

Query: 481 YEVSTISVPDGENVEELRQDQIEPLKPYNIQKGKDVPNCDGVSKNFKRDTTCFFSRAAID 540
           YEVS I+VP+GEN+EE R +QIEPLKPYNIQKGKD+PNCDG SKNFKRDTTCFFSRAA++
Sbjct: 481 YEVSPIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540

Query: 541 FFSNVFTTLFGSVRSTILSGTFTALQSFEDRNEIGLLHEREVLQTCSQSNEMSQIAKLQS 600
           FFSN+FTTLFGSVRSTILSGTFTALQSFE  +EI LL E+EVLQTCSQS EMSQIAKL +
Sbjct: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEINLLCEKEVLQTCSQSTEMSQIAKLHN 600

Query: 601 FGHTNMSIEEIHKNEDLTLSASNENYDLFTQFDMTSDCSDHHFLSSGKGSVLYQTRRGWL 660
           FG T++ IEEIHKNEDLT S S+ENYDL TQFDM SDC DHHFLSSGKG +L Q  R WL
Sbjct: 601 FGETSLDIEEIHKNEDLTFSTSSENYDLLTQFDMASDCPDHHFLSSGKGPMLSQASRRWL 660

Query: 661 KKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQV 720
           KKVQQEWSILKNNLPET+YVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQ+
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720

Query: 721 PPLVHYISGGLRINPNLYESGKVCLSLLNTWTGSGTEVWNAGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLR+NPNLYESG+VCLSLLNTWTGSG+EVWN GSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGRVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSVSYNENAFLITCQSMMYLLTKPPKHFEALVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNS SYNENAFL+TC+SMMYLL+K PKHFEALVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFEALVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYHTGAAELENSNQCSTGFKIMLAKIFPRLIKTFSSKGIDCS 898
           QHILAACRAYMEGTPVGSDY  G  + EN +Q STGFKIMLAKIFPRLIKTFSSKG DCS
Sbjct: 841 QHILAACRAYMEGTPVGSDYQCGTTKPENPDQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900

BLAST of Sed0022645 vs. ExPASy Swiss-Prot
Match: Q8VY10 (Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=UBC24 PE=1 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 6.3e-248
Identity = 455/929 (48.98%), Postives = 617/929 (66.42%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLVEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60
           M++ L+DSDW++SS+  SS+       Y G+   I S+LEETIGKIDEFLSFER FM GD
Sbjct: 1   MEMSLTDSDWDSSSDSGSSEHEEVEFSYGGRAQNIFSNLEETIGKIDEFLSFERGFMYGD 60

Query: 61  VVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISVGDAVVHGPWIGR 120
           +V S  +P GQ G+V+ IDM V+LE  HG ++K+V++K L K+RSIS+ D V++GPW+GR
Sbjct: 61  IVRSATEPSGQSGRVINIDMFVNLESTHGKIMKEVDTKRLQKLRSISLSDYVINGPWVGR 120

Query: 121 VDKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQR--VRFSNVPKPP 180
           VDK+++ VSV  DDGT  E++   Q+KL+ I  + L+DSQY YYPGQR  V+ ++ P+  
Sbjct: 121 VDKIVERVSVTLDDGTNYEVLVDGQDKLVAIPPNLLEDSQYSYYPGQRVQVKLAHAPRST 180

Query: 181 RWLSG-WKDNQCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFPH 240
            WL G W+  Q  GTVC+VEAGLVYVDW+AS++M    +LTAP  +Q+ + LTLL C  H
Sbjct: 181 TWLCGTWRGTQVMGTVCTVEAGLVYVDWVASIVMEGDRNLTAPQALQNPESLTLLPCVSH 240

Query: 241 ANWHLGDWCIFPEDEKNDYLGEKFISN---------------------------EISVIV 300
           A+W LGDWCI P     D + E+   N                           E+ VI 
Sbjct: 241 ASWQLGDWCILPGSSHCD-IAERQTPNVAAYNLNECHKTFQKGFNRNMQNSGLDELFVIT 300

Query: 301 KKKTKVDVLWQDGSSSTGLDSHCLTPANAMNAHEFWPQQLVMDEGHCDDPSMSITQRWGV 360
           K K KV V+WQDGS S G+DS  L P  A+NAH+FWP+Q V+++  C+      +++WGV
Sbjct: 301 KTKMKVAVMWQDGSCSLGVDSQQLLPVGAVNAHDFWPEQFVVEKETCN------SKKWGV 360

Query: 361 VQAVDAKERTVKVQWKMGDLTRGTDLDGPLVEEIMSAYELAEHPDYSFSLGDIVFRLALD 420
           V+AV+AKE+TVKVQW +      T     ++EEI+SAYEL EHPD+ F   D+V +L  +
Sbjct: 361 VKAVNAKEQTVKVQWTIQVEKEATGCVDEVMEEIVSAYELLEHPDFGFCFSDVVVKLLPE 420

Query: 421 KFGGQSDESNINDEISANEESAQKGENRVIDNNMDMDNFISFFGNVTGFKDGAVEVRWAN 480
              G+ D        +A+   A + ++ + +++     F+S  G VTGFK+G+V+V+WAN
Sbjct: 421 ---GKFDP-------NADTIVATEAKHLLTESDYSGAYFLSSIGVVTGFKNGSVKVKWAN 480

Query: 481 CSTTMVPPQEIFPIDKYEVSTISVPDGE-NVEELRQ--DQIEPLKPYNIQKG--KDVPNC 540
            ST+ V P EI+ +++ E S  S    E +V++L Q   Q +     + + G  K     
Sbjct: 481 GSTSKVAPCEIWKMERSEYSNSSTVSSEGSVQDLSQKISQSDEASSNHQETGLVKLYSVG 540

Query: 541 DGVSKNFKRDTTCFFSRAAIDFFSNVFTTLFGSVRSTILSGTFTALQSFEDRNEIGLLHE 600
           +  ++N    ++ F  +AAI F +N+ ++LFG   ST +  + +     ED+++  +L +
Sbjct: 541 ESCNENIPECSSFFLPKAAIGFITNLASSLFGYQGSTSVISSHSRCNDSEDQSDSEVLVQ 600

Query: 601 REVLQTCSQSNEMSQIAKLQSFGHTNMSIEEIHKNEDLTLSASNENYDLFTQFDMTSDCS 660
                  +      ++    +  +  +  + I+K  D TL  ++ N   F QFDM +DCS
Sbjct: 601 ETAESYDNSETNSGEVDMTTTMVNIPIEGKGINKTLDSTLLENSRNQVRFRQFDMVNDCS 660

Query: 661 DHHFLSSGKGSVLYQTRRGWLKKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPG 720
           DHHFLSS KG    Q  + W+KKVQQEWS L+ NLP TIYVRV EERMDL+RA ++GAPG
Sbjct: 661 DHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMDLLRAALVGAPG 720

Query: 721 TPYHDGLFFFDIFLPPEYPQVPPLVHYISGGLRINPNLYESGKVCLSLLNTWTGSGTEVW 780
           TPYHDGLFFFDI LPP+YP  PP+VHY SGG+R+NPNLYESG+VCLSLLNTW+GSGTEVW
Sbjct: 721 TPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLLNTWSGSGTEVW 780

Query: 781 NAGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSVSYNENAFLITCQSMMY 840
           NAGSS+ILQ+LLS QALVLNEKPY+NEAGYD QLGRAEGE+NSVSYNENAFLITC+SM+ 
Sbjct: 781 NAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNENAFLITCKSMIS 840

Query: 841 LLTKPPKHFEALVEEHFRRRYQHILAACRAYMEGTPVGSDYHTGAAELE-NSNQCSTGFK 894
           +L KPPKHFE LV++HF  R QH+LAAC+AYMEG PVGS     +A L+ NS   STGFK
Sbjct: 841 MLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGS-----SANLQGNSTTNSTGFK 900

BLAST of Sed0022645 vs. ExPASy Swiss-Prot
Match: Q9ZVX1 (Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=UBC23 PE=1 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 2.1e-171
Identity = 363/978 (37.12%), Postives = 525/978 (53.68%), Query Frame = 0

Query: 41   ETIGKIDEFLSFEREFMLGDVVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDL 100
            E +  I++    +R F+ GD V S ++P GQ+G VV +++ VDL    G + KD+++K+L
Sbjct: 130  EPVQDINDVTVIDRGFLHGDYVASASEPTGQVGVVVDVNISVDLLAPDGSIHKDISTKNL 189

Query: 101  LKIRSISVGDAVVHGPWIGRVDKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDAL-DDS 160
             ++R  +VGD VVHGPW+GR+D V+D+V+VLFDDG+ C+++ V+  +L PI  + L +D+
Sbjct: 190  KRVRDFAVGDYVVHGPWLGRIDDVLDNVTVLFDDGSMCKVLRVEPLRLKPIPKNNLEEDA 249

Query: 161  QYPYYPGQRVR--FSNVPKPPRWLSG-WKDNQCEGTVCSVEAGLVYVDWLASVLMGCSSS 220
             +PYYPGQRV+   S+V K  RWLSG WK N+ EGTV  V AG ++V W+AS   G  SS
Sbjct: 250  NFPYYPGQRVKASSSSVFKNSRWLSGLWKPNRLEGTVTKVTAGSIFVYWIASAGFGPDSS 309

Query: 221  LTAPPRMQHAKELTLLSCFPHANWHLGDWCIFP----------------------EDEKN 280
            + +PP  Q+   LTLLSCF HANW +GDWC+ P                      + ++ 
Sbjct: 310  V-SPPEEQNPSNLTLLSCFTHANWQVGDWCLLPSLNQSATIPLHKHVSKLRLYDSQADRQ 369

Query: 281  DYLGEKF--ISNEIS--------------------------------------------- 340
              +G     + +E+S                                             
Sbjct: 370  QKIGRDLEDVQDEVSGKVEPAGITAEALPKVTSDDPPQRNPSVSKEPVHEPWPLHRKKIR 429

Query: 341  ---------------------VIVKKKTKVDVLWQDGSSSTGLDSHCLTPANAMNAHEFW 400
                                 ++V  +T+VDV WQDG+     ++  L P      HEF 
Sbjct: 430  KLVIRKDKKVKKKEESFEQALLVVNSRTRVDVSWQDGTIECRREAITLIPIETPGDHEFV 489

Query: 401  PQQLVMDEGHCDDPSMSITQRWGVVQAVDAKERTVKVQWKMGDLTRGTDLDGPLVEEIMS 460
             +Q V+++   D  + +  +R GVV+ V+AK+RT  V+W +  L R  +      EEI+S
Sbjct: 490  SEQYVVEKTSDDGDNTTEPRRAGVVKNVNAKDRTASVRW-LNPLRRAEEPREFEKEEIVS 549

Query: 461  AYELAEHPDYSFSLGDIVFRLA-------LDKFGGQSDESNINDEISANEESAQKGENRV 520
             YEL  HPDY +  GD+V RL+           G   +E+   D    + ES Q+ +  V
Sbjct: 550  VYELEGHPDYDYCYGDVVVRLSPIAVALPASSPGNSFEEATQQDNGYQDSESHQEAKILV 609

Query: 521  --IDNNMDMD-NFISFFGNVTGFKDGAVEVRWANCSTTMVPPQEIFPI-----DKYEVST 580
               +N    D + +S+ GN+TG KDG +EV WA+ + + V P  ++ +     D+     
Sbjct: 610  DKEENEPSTDLSKLSWVGNITGLKDGDIEVTWADGTISTVGPHAVYVVGRDDDDESVAGE 669

Query: 581  ISVPDGENVEELRQDQ--IEPLKPYNIQKGKDVP-NCDG---VSKNFKRDTTCFFSRAAI 640
                D  + E L  D      +   ++ +   +  N D       +  R+       AAI
Sbjct: 670  SETSDAASWETLNDDDRGAPEIPEEDLGRSSSIEGNSDADIYAENDSGRNGALALPLAAI 729

Query: 641  DFFSNVFTTLFGSVRSTI--------LSGTFTALQSFEDRNEIGLLHER-----EVLQTC 700
            +F + + + +F   R ++        +   +   +S    +E   L +       V   C
Sbjct: 730  EFVTRLASGIFSRARKSVDSSSSDYTVENVYKQAESTNPSDETDSLDDPSPSKVNVTDNC 789

Query: 701  SQSNEMSQIAKLQSFGHTNMSIEEIHKNEDLTLSASNENYDLFTQFDMTSDCSDHHFLSS 760
                  +    + S G T+  +E+    ED  + +  ++   F +FD++ D  DHHFL  
Sbjct: 790  ESKGTQANAKNILS-GETSTFLED----EDKPVPSEGDSCS-FRRFDISQDPLDHHFLGV 849

Query: 761  GKGSVLYQTRRGWLKKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGL 820
                      R W KKV Q+W IL+NNLP+ I+VR +E+RMDL+RAVI+GA GTPY DGL
Sbjct: 850  DGQKT---KERQWFKKVDQDWKILQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGL 909

Query: 821  FFFDIFLPPEYPQVPPLVHYISGGLRINPNLYESGKVCLSLLNTWTGSGTEVWNAGSSTI 880
            FFFD  LP +YP VPP  +Y SGG R+NPNLYE GKVCLSLLNTWTG G EVW+  SS+I
Sbjct: 910  FFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSI 969

Query: 881  LQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSVSYNENAFLITCQSMMYLLTKPPK 889
            LQVL+SLQ LVLN KPY+NEAGYD Q+G AEGE+NS+ YNEN FL+ C++MMYL+ KPPK
Sbjct: 970  LQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLGYNENTFLLNCKTMMYLMRKPPK 1029

BLAST of Sed0022645 vs. ExPASy Swiss-Prot
Match: Q8GY87 (Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana OX=3702 GN=UBC26 PE=2 SV=2)

HSP 1 Score: 293.5 bits (750), Expect = 8.1e-78
Identity = 145/270 (53.70%), Postives = 178/270 (65.93%), Query Frame = 0

Query: 615 FTQFDMTSDCSDHHFLSSGKGSVLYQTRRGWLKKVQQEWSILKNNLPETIYVRVFEERMD 674
           F +FD   D SDHH+ S GK S   Q  + W+KKVQ +W IL+N+LPE I VR  E RMD
Sbjct: 244 FKRFDTVEDFSDHHYASKGKSS--KQHSKNWVKKVQADWKILENDLPEAISVRACESRMD 303

Query: 675 LIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQVPPLVHYISGGLRINPNLYESGKVCLSLL 734
           L+RAVIIGA GTPYHDGLFFFDI  P  YP VPP VHY SGGLRINPNLY  GKVCLSLL
Sbjct: 304 LLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLL 363

Query: 735 NTWTGSGTEVWNAGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSVSYNEN 794
            TW GS  E W    ST+LQ+L+S+QAL+LNEKPY+NE GY    G A GE  S  Y+EN
Sbjct: 364 GTWAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESKSKVYSEN 423

Query: 795 AFLITCQSMMYLLTKPPKHFEALVEEHFRRRYQHILAACRAYMEGTPVGSDYHTGAAELE 854
            FL++ ++M+Y + +PP+HFE  V+ H+  R   I+ AC AY  G P+GS    G  +LE
Sbjct: 424 VFLLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLE 483

Query: 855 NSNQC-STGFKIMLAKIFPRLIKTFSSKGI 884
            + Q  S  FK  +A     ++  F   G+
Sbjct: 484 EARQSGSKKFKTDVASFMQTVVDEFVKLGV 511

BLAST of Sed0022645 vs. ExPASy Swiss-Prot
Match: Q9C918 (Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=UBC38 PE=3 SV=2)

HSP 1 Score: 289.3 bits (739), Expect = 1.5e-76
Identity = 145/270 (53.70%), Postives = 178/270 (65.93%), Query Frame = 0

Query: 615 FTQFDMTSDCSDHHFLSSGKGSVLYQTRRGWLKKVQQEWSILKNNLPETIYVRVFEERMD 674
           F +FD   D SDHH++S GK S   Q  + W+KKVQ EW IL  NLPETI+VR  E RMD
Sbjct: 27  FKRFDSVEDFSDHHYVSKGKAS--KQHSKNWVKKVQDEWKILNQNLPETIFVRACESRMD 86

Query: 675 LIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQVPPLVHYISGGLRINPNLYESGKVCLSLL 734
           L+RAVIIGA GTPYHDGLFFFDI  P  YP VPP V+Y SGGLRINPNLY  GKVCLSLL
Sbjct: 87  LLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVYYHSGGLRINPNLYNCGKVCLSLL 146

Query: 735 NTWTGSGTEVWNAGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSVSYNEN 794
            TW G+  + W    ST+LQVL+S+QALVLNE+PY+NE GY    G   GE  S  Y+EN
Sbjct: 147 GTWHGNARQSWLPKESTMLQVLVSIQALVLNEQPYFNEPGYGLIKGTWLGESKSKVYSEN 206

Query: 795 AFLITCQSMMYLLTKPPKHFEALVEEHFRRRYQHILAACRAYMEGTPVGSDYHTGAAELE 854
            FL++ ++M+Y + KPP+HFE  V+ H+  R   I+ AC AY  G P+GS    G  +LE
Sbjct: 207 VFLLSLKTMVYSMRKPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLE 266

Query: 855 NSNQC-STGFKIMLAKIFPRLIKTFSSKGI 884
            + Q  S  FK  +A     ++  F   G+
Sbjct: 267 QARQSGSKKFKTDVASFMQTVVDEFVKLGV 294

BLAST of Sed0022645 vs. ExPASy Swiss-Prot
Match: Q9LUQ5 (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=UBC25 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 1.3e-72
Identity = 131/253 (51.78%), Postives = 171/253 (67.59%), Query Frame = 0

Query: 612 YDLFTQFDMTSDCSDHHFLSSGKGSVLYQTRRGWLKKVQQEWSILKNNLPETIYVRVFEE 671
           Y  F +FD+  D SDHH+ S  KG+   Q  + W K++Q EW IL+ +LPE I+VR +E 
Sbjct: 302 YLFFKRFDIVEDFSDHHYAS--KGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYES 361

Query: 672 RMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQVPPLVHYISGGLRINPNLYESGKVCL 731
           RMDL+RAVIIGA GTPYHDGLFFFDIF P  YP  PP+VHY SGGLRINPNLY  GKVCL
Sbjct: 362 RMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCL 421

Query: 732 SLLNTWTGSGTEVWNAGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSVSY 791
           SLL TW+G+  E W   +ST+LQVL+S+Q L+LN+KPY+NE GY+   G A GE  S +Y
Sbjct: 422 SLLGTWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAY 481

Query: 792 NENAFLITCQSMMYLLTKPPKHFEALVEEHFRRRYQHILAACRAYMEGTPVGSDYHTGAA 851
           +EN F+++ ++M+Y + +PPK+FE     HF      +L AC AY  G   G     GA 
Sbjct: 482 SENTFILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGY-LVKGAP 541

Query: 852 ELENSNQCSTGFK 865
           ++E ++   +  K
Sbjct: 542 DVEENSAGMSSLK 551

BLAST of Sed0022645 vs. ExPASy TrEMBL
Match: A0A6J1CJP8 (probable ubiquitin-conjugating enzyme E2 24 OS=Momordica charantia OX=3673 GN=LOC111012245 PE=4 SV=1)

HSP 1 Score: 1588.9 bits (4113), Expect = 0.0e+00
Identity = 780/912 (85.53%), Postives = 825/912 (90.46%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLVEAEY-YHGQTSCILSSLEETIGKIDEFLSFEREFMLG 60
           MDLFLSDSDWENSSECDSSDD  EAEY YHGQTSCILSSLEETIGKIDEFLSFEREFMLG
Sbjct: 1   MDLFLSDSDWENSSECDSSDDPEEAEYFYHGQTSCILSSLEETIGKIDEFLSFEREFMLG 60

Query: 61  DVVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISVGDAVVHGPWIG 120
           DVVCSVNDPFGQMGKVVGIDMLVDLEKNHGP IKDVNSKDLLKIRSISVGD VVHG WIG
Sbjct: 61  DVVCSVNDPFGQMGKVVGIDMLVDLEKNHGPFIKDVNSKDLLKIRSISVGDVVVHGSWIG 120

Query: 121 RVDKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVPKPPR 180
           RVDKVIDSVSVLFDDGTKCEIIAVDQEKLL ISVDALDD QYPYYPGQRVR SNV K PR
Sbjct: 121 RVDKVIDSVSVLFDDGTKCEIIAVDQEKLLSISVDALDDPQYPYYPGQRVRLSNVSKSPR 180

Query: 181 WLSGWKDNQCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFPHAN 240
           WLSGWKD++CEGTVCSVEAGLVYVDWLASVLMGCSSSL AP RMQHAK+LTLLSCFPHAN
Sbjct: 181 WLSGWKDDRCEGTVCSVEAGLVYVDWLASVLMGCSSSLPAPQRMQHAKKLTLLSCFPHAN 240

Query: 241 WHLGDWCIFPEDEKNDYLGEKFIS--------------NEISVIVKKKTKVDVLWQDGSS 300
           WHLGDWCIFP DEK DYLGEKF +               EIS+IVKKKTKVDVLWQDGS 
Sbjct: 241 WHLGDWCIFPVDEKGDYLGEKFTTTHDLMNGGYCGTGFQEISIIVKKKTKVDVLWQDGSR 300

Query: 301 STGLDSHCLTPANAMNAHEFWPQQLVMDEGHCDDPSMSITQRWGVVQAVDAKERTVKVQW 360
           S+ LDSHCL PANAMNAHEFWPQQLVM++G C+DPS+SI +RWGVV+AVDAKERTVKVQW
Sbjct: 301 SSELDSHCLAPANAMNAHEFWPQQLVMEKGTCNDPSISIAERWGVVRAVDAKERTVKVQW 360

Query: 361 KMGDLTRGTDLDGPLVEEIMSAYELAEHPDYSFSLGDIVFRLALDKFGGQSDESNINDEI 420
           +  DLT G DLD  LVEE MSAYEL EHPDYSFSLGDIVFRLALDKFGGQSDE N N EI
Sbjct: 361 RTSDLTEGNDLDILLVEETMSAYELVEHPDYSFSLGDIVFRLALDKFGGQSDEHNTNYEI 420

Query: 421 SANEESAQKGENRVIDNNMDMDNFISFFGNVTGFKDGAVEVRWANCSTTMVPPQEIFPID 480
           S +EESAQ GENRVIDN M+MDNF+SF GNV GFKDG +EVRWANCST+ V PQEIFPID
Sbjct: 421 SMSEESAQNGENRVIDNTMNMDNFLSFIGNVIGFKDGVMEVRWANCSTSKVLPQEIFPID 480

Query: 481 KYEVSTISVPDGENVEELRQDQIEPLKPYNIQKGKDVPNCDGVSKNFKRDTTCFFSRAAI 540
           KYEVST++VP+GENVEELRQDQIE LKPYNIQKGKD+PNCDG  KNFKRDTTCFFSRAAI
Sbjct: 481 KYEVSTLAVPNGENVEELRQDQIEHLKPYNIQKGKDMPNCDGAGKNFKRDTTCFFSRAAI 540

Query: 541 DFFSNVFTTLFGSVRSTILSGTFTALQSFEDRNEIGLLHEREVLQTCSQSNEMSQIAKLQ 600
           +FFS++FTTLFGSVRSTILSGTFTALQSFE  +EIGLL+E+E LQTC QS E+SQIAKLQ
Sbjct: 541 EFFSSIFTTLFGSVRSTILSGTFTALQSFEGGDEIGLLNEKEELQTCCQSTELSQIAKLQ 600

Query: 601 SFGHTNMSIEEIHKNEDLTLSASNENYDLFTQFDMTSDCSDHHFLSSGKGSVLYQTRRGW 660
            FG T+  IEE HKNED TLS S+ENYDLFTQFDM SDC DHHFLSSG GS+L Q RRGW
Sbjct: 601 DFGETSFKIEENHKNEDFTLSTSSENYDLFTQFDMASDCPDHHFLSSGNGSLLSQARRGW 660

Query: 661 LKKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQ 720
           LKKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQ
Sbjct: 661 LKKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQ 720

Query: 721 VPPLVHYISGGLRINPNLYESGKVCLSLLNTWTGSGTEVWNAGSSTILQVLLSLQALVLN 780
           +PPLVHYISGGLR+NPNLYESGKVCLSLLNTWTGSG+EVWN  SSTILQVLLSLQALVLN
Sbjct: 721 IPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPVSSTILQVLLSLQALVLN 780

Query: 781 EKPYYNEAGYDAQLGRAEGERNSVSYNENAFLITCQSMMYLLTKPPKHFEALVEEHFRRR 840
           EKPYYNEAGYDAQLGRAEGE+NS SYNENAFL+TC+SMMYLL+KPPKHFEALVEEHFRRR
Sbjct: 781 EKPYYNEAGYDAQLGRAEGEKNSDSYNENAFLVTCKSMMYLLSKPPKHFEALVEEHFRRR 840

Query: 841 YQHILAACRAYMEGTPVGSDYHTGAAELENSNQCSTGFKIMLAKIFPRLIKTFSSKGIDC 898
           YQHILAAC AYMEGTPVGSDYH GA +LE  NQ STGFKIMLAKIFP+L++TFSSKGIDC
Sbjct: 841 YQHILAACIAYMEGTPVGSDYHCGATKLEYPNQSSTGFKIMLAKIFPKLVETFSSKGIDC 900

BLAST of Sed0022645 vs. ExPASy TrEMBL
Match: A0A6J1IQQ9 (probable ubiquitin-conjugating enzyme E2 24 OS=Cucurbita maxima OX=3661 GN=LOC111478555 PE=4 SV=1)

HSP 1 Score: 1566.6 bits (4055), Expect = 0.0e+00
Identity = 764/902 (84.70%), Postives = 815/902 (90.35%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLVEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60
           MDL LSDSDWENSS+CDSSDDL EAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD
Sbjct: 1   MDLILSDSDWENSSDCDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60

Query: 61  VVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISVGDAVVHGPWIGR 120
           VVCSVNDPFGQMGKVVGIDMLVDLE NHGP+IKDVNSKDL KIR ISVGD VVHG WIGR
Sbjct: 61  VVCSVNDPFGQMGKVVGIDMLVDLETNHGPIIKDVNSKDLRKIRPISVGDVVVHGKWIGR 120

Query: 121 VDKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVPKPPRW 180
           V+KVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSN  KPPRW
Sbjct: 121 VEKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNASKPPRW 180

Query: 181 LSGWKDNQCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFPHANW 240
           LSGWKDN+CEGTVCSV+AGLVYVDWLAS+LMGCSSSLTAPPRMQHAKELT+LSCFPHANW
Sbjct: 181 LSGWKDNRCEGTVCSVDAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTVLSCFPHANW 240

Query: 241 HLGDWCIFPEDEKNDYLGEKFIS--------------NEISVIVKKKTKVDVLWQDGSSS 300
           HLGDWCIFP DEK+DYLGEKFI+               EISVIVKKKTKVDVLWQDGS S
Sbjct: 241 HLGDWCIFPVDEKSDYLGEKFITAHDLLTGGYRDTGLQEISVIVKKKTKVDVLWQDGSHS 300

Query: 301 TGLDSHCLTPANAMNAHEFWPQQLVMDEGHCDDPSMSITQRWGVVQAVDAKERTVKVQWK 360
            GLDSHCLTPANAMNAHEFWPQQ VM++G+CDD SMS TQRWGVVQAVDAKERTVKVQ +
Sbjct: 301 RGLDSHCLTPANAMNAHEFWPQQFVMEKGNCDDSSMSETQRWGVVQAVDAKERTVKVQLR 360

Query: 361 MGDLTRGTDLDGPLVEEIMSAYELAEHPDYSFSLGDIVFRLALDKFGGQSDESNINDEIS 420
            GDLT G+DLDG  VEE MSAYEL EHPDYSF+LGDIVFRLA   FG QSDE+NI  EIS
Sbjct: 361 TGDLTEGSDLDGLWVEETMSAYELVEHPDYSFTLGDIVFRLA--SFGSQSDENNITSEIS 420

Query: 421 ANEESAQKGENRVIDNNMDMDNFISFFGNVTGFKDGAVEVRWANCSTTMVPPQEIFPIDK 480
            NEESAQ GE+ +I NNM+MDNF+SF GNVT FKDG VEV+WANCS++MV P EIFPIDK
Sbjct: 421 MNEESAQNGESHIISNNMNMDNFLSFIGNVTSFKDGTVEVKWANCSSSMVSPHEIFPIDK 480

Query: 481 YEVSTISVPDGENVEELRQDQIEPLKPYNIQKGKDVPNCDGVSKNFKRDTTCFFSRAAID 540
           YEVS I+VP G+NVEELRQDQIEP KP NIQKGKD+PN D   KNF+RDTTCF SRAAI+
Sbjct: 481 YEVSAIAVPSGDNVEELRQDQIEPSKPDNIQKGKDMPNFDAAGKNFRRDTTCFLSRAAIE 540

Query: 541 FFSNVFTTLFGSVRSTILSGTFTALQSFEDRNEIGLLHEREVLQTCSQSNEMSQIAKLQS 600
           FFSNVFTTLFGSVRSTI+SGTFTALQSFE  + I +L E+EVLQTCSQ    SQIAKL+S
Sbjct: 541 FFSNVFTTLFGSVRSTIISGTFTALQSFEGGDNIDILPEKEVLQTCSQFTGRSQIAKLKS 600

Query: 601 FGHTNMSIEEIHKNEDLTLSASNENYDLFTQFDMTSDCSDHHFLSSGKGSVLYQTRRGWL 660
           FG T+++ EEIH+NE  TLS S+ENYDLFTQFDM SDC DHHFLSSG  S+L Q RRGWL
Sbjct: 601 FGETSLNTEEIHENEASTLSTSSENYDLFTQFDMASDCPDHHFLSSGNASMLSQARRGWL 660

Query: 661 KKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQV 720
           KKVQQEWSILKNNLPET+YVRVFEERMDLIR+VIIGAPGTPYHDGLFFFDIFLPPEYPQ+
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRSVIIGAPGTPYHDGLFFFDIFLPPEYPQI 720

Query: 721 PPLVHYISGGLRINPNLYESGKVCLSLLNTWTGSGTEVWNAGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLR+NPNLYESGKVCLSLLNTWTGSG+EVWN GSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSVSYNENAFLITCQSMMYLLTKPPKHFEALVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNS SYNENAFL+TC+SMMYLL+KPPKHFEALVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEALVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYHTGAAELENSNQCSTGFKIMLAKIFPRLIKTFSSKGIDCS 889
           QHILAACRAYMEGTPVG+DYH+GA +L N NQ STGFKIMLAKI+PRLIKTFSSKGIDCS
Sbjct: 841 QHILAACRAYMEGTPVGADYHSGAKKLVNPNQSSTGFKIMLAKIYPRLIKTFSSKGIDCS 900

BLAST of Sed0022645 vs. ExPASy TrEMBL
Match: A0A1S3B0C7 (probable ubiquitin-conjugating enzyme E2 24 OS=Cucumis melo OX=3656 GN=LOC103484521 PE=4 SV=1)

HSP 1 Score: 1565.1 bits (4051), Expect = 0.0e+00
Identity = 762/911 (83.64%), Postives = 819/911 (89.90%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLVEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60
           MDLFLSDSDWENSSECDSSDDL EAEYYHGQTSCILSSLEETIGKIDEFLSFEREF+LGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  VVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISVGDAVVHGPWIGR 120
            VC VNDP+GQMGKVVGI+MLVDLEKN GPLIK+VNSKDLLKIRSIS GD VV+GPWIGR
Sbjct: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVPKPPRW 180
           VDKVIDSVSVLFDDG KCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNV KPP W
Sbjct: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDNQCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFPHANW 240
           LSGWKD++CEGTVCSVEAGLVYVDWLAS+LMGCSSSLTAPPRMQHAKELTLLSCF HANW
Sbjct: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTLLSCFSHANW 240

Query: 241 HLGDWCIFPEDEKNDYLGEKFISN--------------EISVIVKKKTKVDVLWQDGSSS 300
           +LGDWCIFP DEKN+Y GEKF S               EI +IVKKKTKVDVLWQDGS S
Sbjct: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300

Query: 301 TGLDSHCLTPANAMNAHEFWPQQLVMDEGHCDDPSMSITQRWGVVQAVDAKERTVKVQWK 360
           T LDS CLTPANA NAHEFWPQQ VM++G+CDD SMS+ QRWGVVQAVDAKERTVKVQ +
Sbjct: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 360

Query: 361 MGDLTRGTDLDGPLVEEIMSAYELAEHPDYSFSLGDIVFRLALDKFGGQSDESNINDEIS 420
             DL+ G+ LDG LVEE MSAYEL EHPDYSFSLGDIVFRLA+DKF  QSDE+NI  EIS
Sbjct: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420

Query: 421 ANEESAQKGENRVIDNNMDMDNFISFFGNVTGFKDGAVEVRWANCSTTMVPPQEIFPIDK 480
            NE +  +G + + +NNM+MDNF+SF GNVTGFKDGAVEV+WANCST+MV PQEIFPIDK
Sbjct: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480

Query: 481 YEVSTISVPDGENVEELRQDQIEPLKPYNIQKGKDVPNCDGVSKNFKRDTTCFFSRAAID 540
           YEVS+I+VP+GEN+EE R +QIEPLKPYNIQKGKD+PNCDG SKNFKRDTTCFFSRAA++
Sbjct: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540

Query: 541 FFSNVFTTLFGSVRSTILSGTFTALQSFEDRNEIGLLHEREVLQTCSQSNEMSQIAKLQS 600
           FFSN+FTTLFGSVRSTILSGTFTALQSFE  +EI LL E+EVLQTCSQS EMSQIAKL +
Sbjct: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600

Query: 601 FGHTNMSIEEIHKNEDLTLSASNENYDLFTQFDMTSDCSDHHFLSSGKGSVLYQTRRGWL 660
           FG T++ IEEIHKNEDLT S S++NYDL TQFDM SDC DHHFLSSGKGS+L Q  R WL
Sbjct: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660

Query: 661 KKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQV 720
           KKVQQEWSILKNNLPET+YVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQ+
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720

Query: 721 PPLVHYISGGLRINPNLYESGKVCLSLLNTWTGSGTEVWNAGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLR+NPNLYESGKVCLSLLNTWTGSG+EVWN GSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSVSYNENAFLITCQSMMYLLTKPPKHFEALVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNS SYNENAFL+TC+SMMYLL+K PKHFE LVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYHTGAAELENSNQCSTGFKIMLAKIFPRLIKTFSSKGIDCS 898
           QHILAACRAYMEGTPVGSDY  G  + EN NQ STGFKIMLAKIFPRLIKTFSSKG DCS
Sbjct: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900

BLAST of Sed0022645 vs. ExPASy TrEMBL
Match: A0A5D3CMG4 (Putative ubiquitin-conjugating enzyme E2 24 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007470 PE=4 SV=1)

HSP 1 Score: 1565.1 bits (4051), Expect = 0.0e+00
Identity = 762/911 (83.64%), Postives = 819/911 (89.90%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLVEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60
           MDLFLSDSDWENSSECDSSDDL EAEYYHGQTSCILSSLEETIGKIDEFLSFEREF+LGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  VVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISVGDAVVHGPWIGR 120
            VC VNDP+GQMGKVVGI+MLVDLEKN GPLIK+VNSKDLLKIRSIS GD VV+GPWIGR
Sbjct: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVPKPPRW 180
           VDKVIDSVSVLFDDG KCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNV KPP W
Sbjct: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDNQCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFPHANW 240
           LSGWKD++CEGTVCSVEAGLVYVDWLAS+LMGCSSSLTAPPRMQHAKELTLLSCF HANW
Sbjct: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTLLSCFSHANW 240

Query: 241 HLGDWCIFPEDEKNDYLGEKFISN--------------EISVIVKKKTKVDVLWQDGSSS 300
           +LGDWCIFP DEKN+Y GEKF S               EI +IVKKKTKVDVLWQDGS S
Sbjct: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300

Query: 301 TGLDSHCLTPANAMNAHEFWPQQLVMDEGHCDDPSMSITQRWGVVQAVDAKERTVKVQWK 360
           T LDS CLTPANA NAHEFWPQQ VM++G+CDD SMS+ QRWGVVQAVDAKERTVKVQ +
Sbjct: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 360

Query: 361 MGDLTRGTDLDGPLVEEIMSAYELAEHPDYSFSLGDIVFRLALDKFGGQSDESNINDEIS 420
             DL+ G+ LDG LVEE MSAYEL EHPDYSFSLGDIVFRLA+DKF  QSDE+NI  EIS
Sbjct: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420

Query: 421 ANEESAQKGENRVIDNNMDMDNFISFFGNVTGFKDGAVEVRWANCSTTMVPPQEIFPIDK 480
            NE +  +G + + +NNM+MDNF+SF GNVTGFKDGAVEV+WANCST+MV PQEIFPIDK
Sbjct: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480

Query: 481 YEVSTISVPDGENVEELRQDQIEPLKPYNIQKGKDVPNCDGVSKNFKRDTTCFFSRAAID 540
           YEVS+I+VP+GEN+EE R +QIEPLKPYNIQKGKD+PNCDG SKNFKRDTTCFFSRAA++
Sbjct: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540

Query: 541 FFSNVFTTLFGSVRSTILSGTFTALQSFEDRNEIGLLHEREVLQTCSQSNEMSQIAKLQS 600
           FFSN+FTTLFGSVRSTILSGTFTALQSFE  +EI LL E+EVLQTCSQS EMSQIAKL +
Sbjct: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600

Query: 601 FGHTNMSIEEIHKNEDLTLSASNENYDLFTQFDMTSDCSDHHFLSSGKGSVLYQTRRGWL 660
           FG T++ IEEIHKNEDLT S S++NYDL TQFDM SDC DHHFLSSGKGS+L Q  R WL
Sbjct: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660

Query: 661 KKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQV 720
           KKVQQEWSILKNNLPET+YVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQ+
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720

Query: 721 PPLVHYISGGLRINPNLYESGKVCLSLLNTWTGSGTEVWNAGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLR+NPNLYESGKVCLSLLNTWTGSG+EVWN GSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSVSYNENAFLITCQSMMYLLTKPPKHFEALVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNS SYNENAFL+TC+SMMYLL+K PKHFE LVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYHTGAAELENSNQCSTGFKIMLAKIFPRLIKTFSSKGIDCS 898
           QHILAACRAYMEGTPVGSDY  G  + EN NQ STGFKIMLAKIFPRLIKTFSSKG DCS
Sbjct: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900

BLAST of Sed0022645 vs. ExPASy TrEMBL
Match: A0A0A0KLH0 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517350 PE=4 SV=1)

HSP 1 Score: 1563.1 bits (4046), Expect = 0.0e+00
Identity = 766/911 (84.08%), Postives = 819/911 (89.90%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLVEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60
           MDLFLSDSDWENSSECDSSDDL EAEYYHGQTSCILSSLEETIGKIDEFLSFEREF+LGD
Sbjct: 46  MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 105

Query: 61  VVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISVGDAVVHGPWIGR 120
            VC VNDP+GQMGKVVGIDMLVDLEKN GP+IK+VNSKDLLKIRSIS GD VV+GPWIGR
Sbjct: 106 AVCFVNDPYGQMGKVVGIDMLVDLEKNSGPIIKNVNSKDLLKIRSISAGDVVVNGPWIGR 165

Query: 121 VDKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVPKPPRW 180
           VDKVIDSVSVLFDDG KCEIIAVDQEKLLPISVD LDDSQYPYYPGQRVRFSNV KPP W
Sbjct: 166 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDVLDDSQYPYYPGQRVRFSNVSKPPGW 225

Query: 181 LSGWKDNQCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFPHANW 240
           LSGWKD++CEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCF HANW
Sbjct: 226 LSGWKDDRCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFSHANW 285

Query: 241 HLGDWCIFPEDEKNDYLGEKFIS--------------NEISVIVKKKTKVDVLWQDGSSS 300
           +LGDWCIFP DEKN+Y GEKFI+               +I VIVKKKTKVDVLWQDGS S
Sbjct: 286 NLGDWCIFPGDEKNNYFGEKFITTHDLMNGGYRGTGFQDICVIVKKKTKVDVLWQDGSRS 345

Query: 301 TGLDSHCLTPANAMNAHEFWPQQLVMDEGHCDDPSMSITQRWGVVQAVDAKERTVKVQWK 360
           T LDS CLTPANA NAHEFWPQQ VM++G+CDD SMS+ QRWGVVQAVDAKERTVKVQ +
Sbjct: 346 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 405

Query: 361 MGDLTRGTDLDGPLVEEIMSAYELAEHPDYSFSLGDIVFRLALDKFGGQSDESNINDEIS 420
             DL+ G++LDG LVEE MSAYEL EHPDYSFSLGDIVFRLALDKF  QSDE+NI  EIS
Sbjct: 406 TDDLSEGSNLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLALDKFASQSDENNIISEIS 465

Query: 421 ANEESAQKGENRVIDNNMDMDNFISFFGNVTGFKDGAVEVRWANCSTTMVPPQEIFPIDK 480
            N ESAQ   NR+I+NNM MDNF+SF GNVTGFKDGAVEV+WANCS +MV PQEIF IDK
Sbjct: 466 MN-ESAQNEGNRIINNNMIMDNFLSFIGNVTGFKDGAVEVKWANCSISMVSPQEIFSIDK 525

Query: 481 YEVSTISVPDGENVEELRQDQIEPLKPYNIQKGKDVPNCDGVSKNFKRDTTCFFSRAAID 540
           YEVS I+VP+GEN+EE R +QIEPLKPYNIQKGKD+PNCDG SKNFKRDTTCFFSRAA++
Sbjct: 526 YEVSPIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 585

Query: 541 FFSNVFTTLFGSVRSTILSGTFTALQSFEDRNEIGLLHEREVLQTCSQSNEMSQIAKLQS 600
           FFSN+FTTLFGSVRSTILSGTFTALQSFE  +EI LL E+EVLQTCSQS EMSQIAKL +
Sbjct: 586 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEINLLCEKEVLQTCSQSTEMSQIAKLHN 645

Query: 601 FGHTNMSIEEIHKNEDLTLSASNENYDLFTQFDMTSDCSDHHFLSSGKGSVLYQTRRGWL 660
           FG T++ IEEIHKNEDLT S S+ENYDL TQFDM SDC DHHFLSSGKG +L Q  R WL
Sbjct: 646 FGETSLDIEEIHKNEDLTFSTSSENYDLLTQFDMASDCPDHHFLSSGKGPMLSQASRRWL 705

Query: 661 KKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQV 720
           KKVQQEWSILKNNLPET+YVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQ+
Sbjct: 706 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 765

Query: 721 PPLVHYISGGLRINPNLYESGKVCLSLLNTWTGSGTEVWNAGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLR+NPNLYESG+VCLSLLNTWTGSG+EVWN GSSTILQVLLSLQALVLNE
Sbjct: 766 PPLVHYISGGLRVNPNLYESGRVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 825

Query: 781 KPYYNEAGYDAQLGRAEGERNSVSYNENAFLITCQSMMYLLTKPPKHFEALVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNS SYNENAFL+TC+SMMYLL+K PKHFEALVEEHFRRRY
Sbjct: 826 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFEALVEEHFRRRY 885

Query: 841 QHILAACRAYMEGTPVGSDYHTGAAELENSNQCSTGFKIMLAKIFPRLIKTFSSKGIDCS 898
           QHILAACRAYMEGTPVGSDY  G  + EN +Q STGFKIMLAKIFPRLIKTFSSKG DCS
Sbjct: 886 QHILAACRAYMEGTPVGSDYQCGTTKPENPDQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 945

BLAST of Sed0022645 vs. TAIR 10
Match: AT2G33770.1 (phosphate 2 )

HSP 1 Score: 858.6 bits (2217), Expect = 4.5e-249
Identity = 455/929 (48.98%), Postives = 617/929 (66.42%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLVEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60
           M++ L+DSDW++SS+  SS+       Y G+   I S+LEETIGKIDEFLSFER FM GD
Sbjct: 1   MEMSLTDSDWDSSSDSGSSEHEEVEFSYGGRAQNIFSNLEETIGKIDEFLSFERGFMYGD 60

Query: 61  VVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISVGDAVVHGPWIGR 120
           +V S  +P GQ G+V+ IDM V+LE  HG ++K+V++K L K+RSIS+ D V++GPW+GR
Sbjct: 61  IVRSATEPSGQSGRVINIDMFVNLESTHGKIMKEVDTKRLQKLRSISLSDYVINGPWVGR 120

Query: 121 VDKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQR--VRFSNVPKPP 180
           VDK+++ VSV  DDGT  E++   Q+KL+ I  + L+DSQY YYPGQR  V+ ++ P+  
Sbjct: 121 VDKIVERVSVTLDDGTNYEVLVDGQDKLVAIPPNLLEDSQYSYYPGQRVQVKLAHAPRST 180

Query: 181 RWLSG-WKDNQCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFPH 240
            WL G W+  Q  GTVC+VEAGLVYVDW+AS++M    +LTAP  +Q+ + LTLL C  H
Sbjct: 181 TWLCGTWRGTQVMGTVCTVEAGLVYVDWVASIVMEGDRNLTAPQALQNPESLTLLPCVSH 240

Query: 241 ANWHLGDWCIFPEDEKNDYLGEKFISN---------------------------EISVIV 300
           A+W LGDWCI P     D + E+   N                           E+ VI 
Sbjct: 241 ASWQLGDWCILPGSSHCD-IAERQTPNVAAYNLNECHKTFQKGFNRNMQNSGLDELFVIT 300

Query: 301 KKKTKVDVLWQDGSSSTGLDSHCLTPANAMNAHEFWPQQLVMDEGHCDDPSMSITQRWGV 360
           K K KV V+WQDGS S G+DS  L P  A+NAH+FWP+Q V+++  C+      +++WGV
Sbjct: 301 KTKMKVAVMWQDGSCSLGVDSQQLLPVGAVNAHDFWPEQFVVEKETCN------SKKWGV 360

Query: 361 VQAVDAKERTVKVQWKMGDLTRGTDLDGPLVEEIMSAYELAEHPDYSFSLGDIVFRLALD 420
           V+AV+AKE+TVKVQW +      T     ++EEI+SAYEL EHPD+ F   D+V +L  +
Sbjct: 361 VKAVNAKEQTVKVQWTIQVEKEATGCVDEVMEEIVSAYELLEHPDFGFCFSDVVVKLLPE 420

Query: 421 KFGGQSDESNINDEISANEESAQKGENRVIDNNMDMDNFISFFGNVTGFKDGAVEVRWAN 480
              G+ D        +A+   A + ++ + +++     F+S  G VTGFK+G+V+V+WAN
Sbjct: 421 ---GKFDP-------NADTIVATEAKHLLTESDYSGAYFLSSIGVVTGFKNGSVKVKWAN 480

Query: 481 CSTTMVPPQEIFPIDKYEVSTISVPDGE-NVEELRQ--DQIEPLKPYNIQKG--KDVPNC 540
            ST+ V P EI+ +++ E S  S    E +V++L Q   Q +     + + G  K     
Sbjct: 481 GSTSKVAPCEIWKMERSEYSNSSTVSSEGSVQDLSQKISQSDEASSNHQETGLVKLYSVG 540

Query: 541 DGVSKNFKRDTTCFFSRAAIDFFSNVFTTLFGSVRSTILSGTFTALQSFEDRNEIGLLHE 600
           +  ++N    ++ F  +AAI F +N+ ++LFG   ST +  + +     ED+++  +L +
Sbjct: 541 ESCNENIPECSSFFLPKAAIGFITNLASSLFGYQGSTSVISSHSRCNDSEDQSDSEVLVQ 600

Query: 601 REVLQTCSQSNEMSQIAKLQSFGHTNMSIEEIHKNEDLTLSASNENYDLFTQFDMTSDCS 660
                  +      ++    +  +  +  + I+K  D TL  ++ N   F QFDM +DCS
Sbjct: 601 ETAESYDNSETNSGEVDMTTTMVNIPIEGKGINKTLDSTLLENSRNQVRFRQFDMVNDCS 660

Query: 661 DHHFLSSGKGSVLYQTRRGWLKKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPG 720
           DHHFLSS KG    Q  + W+KKVQQEWS L+ NLP TIYVRV EERMDL+RA ++GAPG
Sbjct: 661 DHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMDLLRAALVGAPG 720

Query: 721 TPYHDGLFFFDIFLPPEYPQVPPLVHYISGGLRINPNLYESGKVCLSLLNTWTGSGTEVW 780
           TPYHDGLFFFDI LPP+YP  PP+VHY SGG+R+NPNLYESG+VCLSLLNTW+GSGTEVW
Sbjct: 721 TPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLLNTWSGSGTEVW 780

Query: 781 NAGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSVSYNENAFLITCQSMMY 840
           NAGSS+ILQ+LLS QALVLNEKPY+NEAGYD QLGRAEGE+NSVSYNENAFLITC+SM+ 
Sbjct: 781 NAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNENAFLITCKSMIS 840

Query: 841 LLTKPPKHFEALVEEHFRRRYQHILAACRAYMEGTPVGSDYHTGAAELE-NSNQCSTGFK 894
           +L KPPKHFE LV++HF  R QH+LAAC+AYMEG PVGS     +A L+ NS   STGFK
Sbjct: 841 MLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGS-----SANLQGNSTTNSTGFK 900

BLAST of Sed0022645 vs. TAIR 10
Match: AT2G16920.1 (ubiquitin-conjugating enzyme 23 )

HSP 1 Score: 604.4 bits (1557), Expect = 1.5e-172
Identity = 363/978 (37.12%), Postives = 525/978 (53.68%), Query Frame = 0

Query: 41   ETIGKIDEFLSFEREFMLGDVVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDL 100
            E +  I++    +R F+ GD V S ++P GQ+G VV +++ VDL    G + KD+++K+L
Sbjct: 130  EPVQDINDVTVIDRGFLHGDYVASASEPTGQVGVVVDVNISVDLLAPDGSIHKDISTKNL 189

Query: 101  LKIRSISVGDAVVHGPWIGRVDKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDAL-DDS 160
             ++R  +VGD VVHGPW+GR+D V+D+V+VLFDDG+ C+++ V+  +L PI  + L +D+
Sbjct: 190  KRVRDFAVGDYVVHGPWLGRIDDVLDNVTVLFDDGSMCKVLRVEPLRLKPIPKNNLEEDA 249

Query: 161  QYPYYPGQRVR--FSNVPKPPRWLSG-WKDNQCEGTVCSVEAGLVYVDWLASVLMGCSSS 220
             +PYYPGQRV+   S+V K  RWLSG WK N+ EGTV  V AG ++V W+AS   G  SS
Sbjct: 250  NFPYYPGQRVKASSSSVFKNSRWLSGLWKPNRLEGTVTKVTAGSIFVYWIASAGFGPDSS 309

Query: 221  LTAPPRMQHAKELTLLSCFPHANWHLGDWCIFP----------------------EDEKN 280
            + +PP  Q+   LTLLSCF HANW +GDWC+ P                      + ++ 
Sbjct: 310  V-SPPEEQNPSNLTLLSCFTHANWQVGDWCLLPSLNQSATIPLHKHVSKLRLYDSQADRQ 369

Query: 281  DYLGEKF--ISNEIS--------------------------------------------- 340
              +G     + +E+S                                             
Sbjct: 370  QKIGRDLEDVQDEVSGKVEPAGITAEALPKVTSDDPPQRNPSVSKEPVHEPWPLHRKKIR 429

Query: 341  ---------------------VIVKKKTKVDVLWQDGSSSTGLDSHCLTPANAMNAHEFW 400
                                 ++V  +T+VDV WQDG+     ++  L P      HEF 
Sbjct: 430  KLVIRKDKKVKKKEESFEQALLVVNSRTRVDVSWQDGTIECRREAITLIPIETPGDHEFV 489

Query: 401  PQQLVMDEGHCDDPSMSITQRWGVVQAVDAKERTVKVQWKMGDLTRGTDLDGPLVEEIMS 460
             +Q V+++   D  + +  +R GVV+ V+AK+RT  V+W +  L R  +      EEI+S
Sbjct: 490  SEQYVVEKTSDDGDNTTEPRRAGVVKNVNAKDRTASVRW-LNPLRRAEEPREFEKEEIVS 549

Query: 461  AYELAEHPDYSFSLGDIVFRLA-------LDKFGGQSDESNINDEISANEESAQKGENRV 520
             YEL  HPDY +  GD+V RL+           G   +E+   D    + ES Q+ +  V
Sbjct: 550  VYELEGHPDYDYCYGDVVVRLSPIAVALPASSPGNSFEEATQQDNGYQDSESHQEAKILV 609

Query: 521  --IDNNMDMD-NFISFFGNVTGFKDGAVEVRWANCSTTMVPPQEIFPI-----DKYEVST 580
               +N    D + +S+ GN+TG KDG +EV WA+ + + V P  ++ +     D+     
Sbjct: 610  DKEENEPSTDLSKLSWVGNITGLKDGDIEVTWADGTISTVGPHAVYVVGRDDDDESVAGE 669

Query: 581  ISVPDGENVEELRQDQ--IEPLKPYNIQKGKDVP-NCDG---VSKNFKRDTTCFFSRAAI 640
                D  + E L  D      +   ++ +   +  N D       +  R+       AAI
Sbjct: 670  SETSDAASWETLNDDDRGAPEIPEEDLGRSSSIEGNSDADIYAENDSGRNGALALPLAAI 729

Query: 641  DFFSNVFTTLFGSVRSTI--------LSGTFTALQSFEDRNEIGLLHER-----EVLQTC 700
            +F + + + +F   R ++        +   +   +S    +E   L +       V   C
Sbjct: 730  EFVTRLASGIFSRARKSVDSSSSDYTVENVYKQAESTNPSDETDSLDDPSPSKVNVTDNC 789

Query: 701  SQSNEMSQIAKLQSFGHTNMSIEEIHKNEDLTLSASNENYDLFTQFDMTSDCSDHHFLSS 760
                  +    + S G T+  +E+    ED  + +  ++   F +FD++ D  DHHFL  
Sbjct: 790  ESKGTQANAKNILS-GETSTFLED----EDKPVPSEGDSCS-FRRFDISQDPLDHHFLGV 849

Query: 761  GKGSVLYQTRRGWLKKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGL 820
                      R W KKV Q+W IL+NNLP+ I+VR +E+RMDL+RAVI+GA GTPY DGL
Sbjct: 850  DGQKT---KERQWFKKVDQDWKILQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGL 909

Query: 821  FFFDIFLPPEYPQVPPLVHYISGGLRINPNLYESGKVCLSLLNTWTGSGTEVWNAGSSTI 880
            FFFD  LP +YP VPP  +Y SGG R+NPNLYE GKVCLSLLNTWTG G EVW+  SS+I
Sbjct: 910  FFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSI 969

Query: 881  LQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSVSYNENAFLITCQSMMYLLTKPPK 889
            LQVL+SLQ LVLN KPY+NEAGYD Q+G AEGE+NS+ YNEN FL+ C++MMYL+ KPPK
Sbjct: 970  LQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLGYNENTFLLNCKTMMYLMRKPPK 1029

BLAST of Sed0022645 vs. TAIR 10
Match: AT1G53025.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 293.5 bits (750), Expect = 5.7e-79
Identity = 145/270 (53.70%), Postives = 178/270 (65.93%), Query Frame = 0

Query: 615 FTQFDMTSDCSDHHFLSSGKGSVLYQTRRGWLKKVQQEWSILKNNLPETIYVRVFEERMD 674
           F +FD   D SDHH+ S GK S   Q  + W+KKVQ +W IL+N+LPE I VR  E RMD
Sbjct: 244 FKRFDTVEDFSDHHYASKGKSS--KQHSKNWVKKVQADWKILENDLPEAISVRACESRMD 303

Query: 675 LIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQVPPLVHYISGGLRINPNLYESGKVCLSLL 734
           L+RAVIIGA GTPYHDGLFFFDI  P  YP VPP VHY SGGLRINPNLY  GKVCLSLL
Sbjct: 304 LLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLL 363

Query: 735 NTWTGSGTEVWNAGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSVSYNEN 794
            TW GS  E W    ST+LQ+L+S+QAL+LNEKPY+NE GY    G A GE  S  Y+EN
Sbjct: 364 GTWAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESKSKVYSEN 423

Query: 795 AFLITCQSMMYLLTKPPKHFEALVEEHFRRRYQHILAACRAYMEGTPVGSDYHTGAAELE 854
            FL++ ++M+Y + +PP+HFE  V+ H+  R   I+ AC AY  G P+GS    G  +LE
Sbjct: 424 VFLLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLE 483

Query: 855 NSNQC-STGFKIMLAKIFPRLIKTFSSKGI 884
            + Q  S  FK  +A     ++  F   G+
Sbjct: 484 EARQSGSKKFKTDVASFMQTVVDEFVKLGV 511

BLAST of Sed0022645 vs. TAIR 10
Match: AT3G15355.1 (ubiquitin-conjugating enzyme 25 )

HSP 1 Score: 276.2 bits (705), Expect = 9.5e-74
Identity = 131/253 (51.78%), Postives = 171/253 (67.59%), Query Frame = 0

Query: 612 YDLFTQFDMTSDCSDHHFLSSGKGSVLYQTRRGWLKKVQQEWSILKNNLPETIYVRVFEE 671
           Y  F +FD+  D SDHH+ S  KG+   Q  + W K++Q EW IL+ +LPE I+VR +E 
Sbjct: 302 YLFFKRFDIVEDFSDHHYAS--KGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYES 361

Query: 672 RMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQVPPLVHYISGGLRINPNLYESGKVCL 731
           RMDL+RAVIIGA GTPYHDGLFFFDIF P  YP  PP+VHY SGGLRINPNLY  GKVCL
Sbjct: 362 RMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCL 421

Query: 732 SLLNTWTGSGTEVWNAGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSVSY 791
           SLL TW+G+  E W   +ST+LQVL+S+Q L+LN+KPY+NE GY+   G A GE  S +Y
Sbjct: 422 SLLGTWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAY 481

Query: 792 NENAFLITCQSMMYLLTKPPKHFEALVEEHFRRRYQHILAACRAYMEGTPVGSDYHTGAA 851
           +EN F+++ ++M+Y + +PPK+FE     HF      +L AC AY  G   G     GA 
Sbjct: 482 SENTFILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGY-LVKGAP 541

Query: 852 ELENSNQCSTGFK 865
           ++E ++   +  K
Sbjct: 542 DVEENSAGMSSLK 551

BLAST of Sed0022645 vs. TAIR 10
Match: AT1G53023.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 265.0 bits (676), Expect = 2.2e-70
Identity = 135/275 (49.09%), Postives = 173/275 (62.91%), Query Frame = 0

Query: 615 FTQFDMTSDCSDHHFLSSGKGSVL------YQTR-RGWLKKVQQEWSILKNNLPETIYVR 674
           F +FD   D SDHH+ + G  + L      ++ R + W+K +Q+EW IL  NLPETI+VR
Sbjct: 21  FKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILDKNLPETIFVR 80

Query: 675 VFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQVPPLVHYISGGLRINPNLYESG 734
             E R+DL+RAVIIGA GTPYHDGLFFFDI  P  YP VPP VHY SGGLRINPNLY+ G
Sbjct: 81  ACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHSGGLRINPNLYKCG 140

Query: 735 KVCLSLLNTWTGSGTEVWNAGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERN 794
           KVCLSL++TWTG   E W    ST+LQ+L+S+QAL+LNEKPYYNE GY+  +G   GE  
Sbjct: 141 KVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEKSMGTPLGESY 200

Query: 795 SVSYNENAFLITCQSMMYLLTKPPKHFEALVEEHFRRRYQHILAACRAYMEGTPVGSDYH 854
           S  Y+EN F+ + ++M         HFE  V  HF  R   I+ AC AY +G PVGS   
Sbjct: 201 SKDYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDIVKACNAYKDGAPVGS-ID 260

Query: 855 TGAAELENSNQCSTGFKIMLAKIFPRLIKTFSSKG 883
            G  + +   + S  F+I +      ++  F + G
Sbjct: 261 KGGVKKQTRQRGSLKFRINVTSFMKTVVDEFVNLG 285