Sed0022633 (gene) Chayote v1

BLAST of Sed0022633 vs. NCBI nr
Match: XP_004145358.2 (2-hydroxyisoflavanone dehydratase [Cucumis sativus] >KGN61726.1 hypothetical protein Csa_006817 [Cucumis sativus])

HSP 1 Score: 266.2 bits (679), Expect = 2.8e-67
Identity = 138/214 (64.49%), Postives = 159/214 (74.30%), Query Frame = 0

Query: 6   IIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFIPCS 65
           +  +F PFLR+Y DGRV+RL  TSD VPA ADDP S FRSKDVTI  DPAVSARVFIP S
Sbjct: 11  VAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSARVFIPSS 70

Query: 66  AEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIPACY 125
           A+P Q+LP+L++VHGGAFC  SAF+L +H HVGSL+ KANAVAV+VEYRLAPEHPIPACY
Sbjct: 71  ADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPEHPIPACY 130

Query: 126 DDCWDALRWVAAHVN-SILEGWSRTMVEGACRLQPCDLSRGQR--HHVAARAGSSPDHLG 185
           +DCWDALRWVAAHVN    E W  T V+   R+     S G    H++AARA SS + LG
Sbjct: 131 EDCWDALRWVAAHVNRDGSEPWLNTYVD-FNRICLAGDSAGANICHYLAARASSSAEELG 190

Query: 186 GVKVLAMALIHVFFGDGGRRQ-----AVETSLLR 212
           G KV+AMALIH FFGDGG  +       ET LLR
Sbjct: 191 GAKVVAMALIHPFFGDGGENRLWKYLCSETKLLR 223

BLAST of Sed0022633 vs. NCBI nr
Match: XP_008459211.1 (PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Cucumis melo] >KAA0032058.1 2-hydroxyisoflavanone dehydratase-like [Cucumis melo var. makuwa] >TYK13561.1 2-hydroxyisoflavanone dehydratase-like [Cucumis melo var. makuwa])

HSP 1 Score: 265.8 bits (678), Expect = 3.6e-67
Identity = 139/226 (61.50%), Postives = 164/226 (72.57%), Query Frame = 0

Query: 6   IIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFIPCS 65
           +  +  PFLR+Y+DGRV+RL ITSD VPA ADDP S FRSKDVTI  DPAVSARVFIP S
Sbjct: 11  VAFDLFPFLRVYSDGRVQRLMITSDFVPADADDPKSPFRSKDVTISTDPAVSARVFIPSS 70

Query: 66  AEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIPACY 125
           A+P Q+LP+L++VHGGAFC  SAFNL +H HVG+L+ +ANAVAV+VEYRLAPEHPIPACY
Sbjct: 71  ADPNQKLPLLLYVHGGAFCIESAFNLQYHQHVGALAAEANAVAVSVEYRLAPEHPIPACY 130

Query: 126 DDCWDALRWVAAHVN-SILEGWSRTMVEGACRLQPCDLSRGQR--HHVAARAGSSPDHLG 185
           DDCWDALRWVAAHVN   LE W  T V+   R+     S G    H++AARA SS + LG
Sbjct: 131 DDCWDALRWVAAHVNRDGLEPWLNTYVD-FNRICLAGDSAGANICHYLAARASSSSEELG 190

Query: 186 GVKVLAMALIHVFFGDGGRRQ-----AVETSLLRTPKPSASARGAR 224
           G KV+AMALIH FFGDG   +       ET L+R  K   +  G +
Sbjct: 191 GAKVVAMALIHPFFGDGREDRLWKYLCSETRLVRPTKEELAKMGCK 235

BLAST of Sed0022633 vs. NCBI nr
Match: XP_023512082.1 (probable carboxylesterase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 251.5 bits (641), Expect = 7.1e-63
Identity = 125/197 (63.45%), Postives = 149/197 (75.63%), Query Frame = 0

Query: 6   IIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFIPCS 65
           +  EF PFLRIY DGR++RLQ+TSD VPA ADDP S FR+KDVTI  D A+S R+FIP +
Sbjct: 8   VADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSVRIFIPSA 67

Query: 66  AEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIPACY 125
            +  Q+LP+L++VHGGAFCF SAFNL +H HVGSL+ KANAVAV+VEYRLAPE+PIPACY
Sbjct: 68  VDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENPIPACY 127

Query: 126 DDCWDALRWVAAHVN-SILEGWSRTMVEGACRLQPCDLSRGQR--HHVAARAGSSPDHLG 185
           DDCWDALRW+AAHVN    E W     +   R+     S G    HH+A RAGSS + LG
Sbjct: 128 DDCWDALRWIAAHVNRDGPEPWLNQHAD-LDRICLAGDSAGANICHHLAVRAGSSAEQLG 187

Query: 186 GVKVLAMALIHVFFGDG 200
            VKV+A+ALIH FFGDG
Sbjct: 188 RVKVVALALIHPFFGDG 203

BLAST of Sed0022633 vs. NCBI nr
Match: XP_022986577.1 (probable carboxylesterase 12 [Cucurbita maxima] >XP_022986578.1 probable carboxylesterase 12 [Cucurbita maxima])

HSP 1 Score: 251.5 bits (641), Expect = 7.1e-63
Identity = 124/198 (62.63%), Postives = 150/198 (75.76%), Query Frame = 0

Query: 6   IIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFIPCS 65
           +  EF PFLRIY +GR++RLQ+TSD VPA ADDPNS FR+KDVTI  D A+S R+FIP +
Sbjct: 8   VADEFFPFLRIYKNGRIQRLQVTSDIVPACADDPNSAFRAKDVTISVDSALSVRIFIPSA 67

Query: 66  AEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIPACY 125
            +  Q+LP+L++VHGGAFCF SAFNL +H HVGSL+ KANAVAV+VEYRLAPE+PIPACY
Sbjct: 68  VDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENPIPACY 127

Query: 126 DDCWDALRWVAAHVNSILEGWSRTMVEGACRLQPC----DLSRGQRHHVAARAGSSPDHL 185
           DDCWDALRW+AAHVN   +G    + E A   + C           HH+A RAGSS + L
Sbjct: 128 DDCWDALRWIAAHVNR--DGPEPWLNEHADLDRICLAGDSAGANICHHLAVRAGSSAEQL 187

Query: 186 GGVKVLAMALIHVFFGDG 200
           G VKV+A+ALIH FFGDG
Sbjct: 188 GRVKVVALALIHPFFGDG 203

BLAST of Sed0022633 vs. NCBI nr
Match: XP_022944136.1 (probable carboxylesterase 12 [Cucurbita moschata])

HSP 1 Score: 248.8 bits (634), Expect = 4.6e-62
Identity = 124/197 (62.94%), Postives = 149/197 (75.63%), Query Frame = 0

Query: 6   IIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFIPCS 65
           +  EF PFLRIY DGR++RLQ+TSD V A ADDPNS FR+KDVTI  D A+S R+FIP +
Sbjct: 8   VADEFFPFLRIYKDGRIQRLQVTSDIVSACADDPNSAFRAKDVTISVDSALSVRIFIPSA 67

Query: 66  AEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIPACY 125
            +  Q+LP+L++VHGGAFCF SAFNL +H HVGSL+ KANAVAV+VEYRLAPE+PIPACY
Sbjct: 68  VDLNQKLPLLLYVHGGAFCFESAFNLQYHRHVGSLAAKANAVAVSVEYRLAPENPIPACY 127

Query: 126 DDCWDALRWVAAHVN-SILEGWSRTMVEGACRLQPCDLSRGQR--HHVAARAGSSPDHLG 185
           DDCWDALRW+AAHVN    E W     +   R+     S G    HH+A RAG+S + LG
Sbjct: 128 DDCWDALRWIAAHVNRDGPEPWLNQHAD-LDRICLAGDSAGANICHHLAVRAGTSAEQLG 187

Query: 186 GVKVLAMALIHVFFGDG 200
            VKV+A+ALIH FFGDG
Sbjct: 188 RVKVVALALIHPFFGDG 203

BLAST of Sed0022633 vs. ExPASy Swiss-Prot
Match: Q5NUF3 (2-hydroxyisoflavanone dehydratase OS=Glycine max OX=3847 GN=HIDH PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.9e-34
Identity = 64/139 (46.04%), Postives = 96/139 (69.06%), Query Frame = 0

Query: 3   SKTIIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFI 62
           +K I+ E LP +R+Y DG VERL ++S+ V A  +DP +G  SKD+ I  +P VSAR+F+
Sbjct: 2   AKEIVKELLPLIRVYKDGSVERL-LSSENVAASPEDPQTGVSSKDIVIADNPYVSARIFL 61

Query: 63  PCSAEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIP 122
           P S     +LP+ ++ HGGAFC  SAF+   H ++  L+ +AN +A++V++RL P HPIP
Sbjct: 62  PKSHHTNNKLPIFLYFHGGAFCVESAFSFFVHRYLNILASEANIIAISVDFRLLPHHPIP 121

Query: 123 ACYDDCWDALRWVAAHVNS 142
           A Y+D W  L+W+A+H N+
Sbjct: 122 AAYEDGWTTLKWIASHANN 139

BLAST of Sed0022633 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 3.0e-32
Identity = 64/136 (47.06%), Postives = 91/136 (66.91%), Query Frame = 0

Query: 3   SKTIIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFI 62
           SK I  E  P LR+Y DG VER  + S  VP   +DP +G  +KD+ I  +P +SARV++
Sbjct: 10  SKEIDRELPPLLRVYKDGTVERF-LGSSFVPPSPEDPETGVSTKDIVISENPTISARVYL 69

Query: 63  PCSAEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIP 122
           P      ++LP+LV+ HGGAFC  SAF+ +   ++  ++ KAN + V++EYRLAPEHP+P
Sbjct: 70  PKLNNTTEKLPILVYYHGGAFCLESAFSFLHQRYLNIVASKANVLVVSIEYRLAPEHPLP 129

Query: 123 ACYDDCWDALRWVAAH 139
           A Y+D W AL+WV +H
Sbjct: 130 AAYEDGWYALKWVTSH 144

BLAST of Sed0022633 vs. ExPASy Swiss-Prot
Match: A0A2P1GIY2 (Hydrolase 3 OS=Catharanthus roseus OX=4058 GN=HL3 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 5.1e-32
Identity = 80/207 (38.65%), Postives = 113/207 (54.59%), Query Frame = 0

Query: 3   SKTIIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFI 62
           S   I +  P++R++ DGRVERL  +S  VP   +DP +G   KDV I +   VSAR+++
Sbjct: 4   SDETIFDLPPYIRVFKDGRVERLH-SSPYVPPSLNDPETGVSWKDVPISS--KVSARIYL 63

Query: 63  P---CSAEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEH 122
           P      E  ++LP+ V+ HG  FC  SAF   FH  +     +A A+ V+VEYRLAPEH
Sbjct: 64  PKISDQQENEEKLPIFVYFHGAGFCLESAFRSFFHTFIKHFVSEAKAIGVSVEYRLAPEH 123

Query: 123 PIPACYDDCWDALRWVAAHVNSILEGWSRTMVEGACRLQPCDLSR----------GQRHH 182
           P+PA Y+DCW+AL+WVA+HV        R+M +    +   D  R             H+
Sbjct: 124 PLPAAYEDCWEALQWVASHVRLDNSSLKRSMDKDPWIINYGDFDRLYLGGDSPGGNIVHN 183

Query: 183 VAARAGSSPDHLGGVKVLAMALIHVFF 197
           V  RAG    + GGVK+L     + +F
Sbjct: 184 VLLRAGKEKLN-GGVKILGAIQYYPYF 206

BLAST of Sed0022633 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW2 (Catharanthine synthase OS=Catharanthus roseus OX=4058 GN=CS PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.7e-30
Identity = 78/204 (38.24%), Postives = 107/204 (52.45%), Query Frame = 0

Query: 3   SKTIIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFI 62
           S   I +  P+++I+ DGRVERL   S  VP   +DP +G   KDV I +   VSARV+I
Sbjct: 9   SDETIWDLSPYIKIFKDGRVERLH-NSPYVPPSLNDPETGVSWKDVPISSQ--VSARVYI 68

Query: 63  PCSAEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIP 122
           P      ++LP+ V+VHG  FC  SAF   FH  V     +   + V++EYRLAPEH +P
Sbjct: 69  P-KISDHEKLPIFVYVHGAGFCLESAFRSFFHTFVKHFVAETKVIGVSIEYRLAPEHLLP 128

Query: 123 ACYDDCWDALRWVAAHVNSILEGWSRTMVEGACRLQPCDLSR----------GQRHHVAA 182
           A Y+DCW+AL+WVA+HV     G    + +    +   D  R             H+   
Sbjct: 129 AAYEDCWEALQWVASHVGLDNSGLKTAIDKDPWIINYGDFDRLYLAGDSPGANIVHNTLI 188

Query: 183 RAGSSPDHLGGVKVLAMALIHVFF 197
           RAG      GGVK+L   L + +F
Sbjct: 189 RAGKE-KLKGGVKILGAILYYPYF 207

BLAST of Sed0022633 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW3 (Tabersonine synthase OS=Catharanthus roseus OX=4058 GN=TS PE=1 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 3.7e-30
Identity = 74/205 (36.10%), Postives = 113/205 (55.12%), Query Frame = 0

Query: 3   SKTIIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRS-KDVTIKADPAVSARVF 62
           S   I +  P+++++ DGRVERL  +S  VP   +DP +G  S KDV I +   VSAR++
Sbjct: 4   SDETIFDLPPYIKVFKDGRVERLH-SSPYVPPSLNDPETGGVSWKDVPISS--VVSARIY 63

Query: 63  IPCSAEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPI 122
           +P      ++LP++V+ HG  FC  SAF   FH +V     +A A+AV+VE+RLAPE+ +
Sbjct: 64  LPKINNHDEKLPIIVYFHGAGFCLESAFKSFFHTYVKHFVAEAKAIAVSVEFRLAPENHL 123

Query: 123 PACYDDCWDALRWVAAHVNSILEGWSRTMVEGACRLQPCDLSR----------GQRHHVA 182
           PA Y+DCW+AL+WVA+HV   +      + +    +   D  R             H+  
Sbjct: 124 PAAYEDCWEALQWVASHVGLDISSLKTCIDKDPWIINYADFDRLYLWGDSTGANIVHNTL 183

Query: 183 ARAGSSPDHLGGVKVLAMALIHVFF 197
            R+G    + G VK+L   L + +F
Sbjct: 184 IRSGKEKLNGGKVKILGAILYYPYF 205

BLAST of Sed0022633 vs. ExPASy TrEMBL
Match: A0A0A0LNZ1 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G234530 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 1.3e-67
Identity = 138/214 (64.49%), Postives = 159/214 (74.30%), Query Frame = 0

Query: 6   IIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFIPCS 65
           +  +F PFLR+Y DGRV+RL  TSD VPA ADDP S FRSKDVTI  DPAVSARVFIP S
Sbjct: 11  VAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSARVFIPSS 70

Query: 66  AEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIPACY 125
           A+P Q+LP+L++VHGGAFC  SAF+L +H HVGSL+ KANAVAV+VEYRLAPEHPIPACY
Sbjct: 71  ADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPEHPIPACY 130

Query: 126 DDCWDALRWVAAHVN-SILEGWSRTMVEGACRLQPCDLSRGQR--HHVAARAGSSPDHLG 185
           +DCWDALRWVAAHVN    E W  T V+   R+     S G    H++AARA SS + LG
Sbjct: 131 EDCWDALRWVAAHVNRDGSEPWLNTYVD-FNRICLAGDSAGANICHYLAARASSSAEELG 190

Query: 186 GVKVLAMALIHVFFGDGGRRQ-----AVETSLLR 212
           G KV+AMALIH FFGDGG  +       ET LLR
Sbjct: 191 GAKVVAMALIHPFFGDGGENRLWKYLCSETKLLR 223

BLAST of Sed0022633 vs. ExPASy TrEMBL
Match: A0A5D3CP06 (2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G00380 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.8e-67
Identity = 139/226 (61.50%), Postives = 164/226 (72.57%), Query Frame = 0

Query: 6   IIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFIPCS 65
           +  +  PFLR+Y+DGRV+RL ITSD VPA ADDP S FRSKDVTI  DPAVSARVFIP S
Sbjct: 11  VAFDLFPFLRVYSDGRVQRLMITSDFVPADADDPKSPFRSKDVTISTDPAVSARVFIPSS 70

Query: 66  AEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIPACY 125
           A+P Q+LP+L++VHGGAFC  SAFNL +H HVG+L+ +ANAVAV+VEYRLAPEHPIPACY
Sbjct: 71  ADPNQKLPLLLYVHGGAFCIESAFNLQYHQHVGALAAEANAVAVSVEYRLAPEHPIPACY 130

Query: 126 DDCWDALRWVAAHVN-SILEGWSRTMVEGACRLQPCDLSRGQR--HHVAARAGSSPDHLG 185
           DDCWDALRWVAAHVN   LE W  T V+   R+     S G    H++AARA SS + LG
Sbjct: 131 DDCWDALRWVAAHVNRDGLEPWLNTYVD-FNRICLAGDSAGANICHYLAARASSSSEELG 190

Query: 186 GVKVLAMALIHVFFGDGGRRQ-----AVETSLLRTPKPSASARGAR 224
           G KV+AMALIH FFGDG   +       ET L+R  K   +  G +
Sbjct: 191 GAKVVAMALIHPFFGDGREDRLWKYLCSETRLVRPTKEELAKMGCK 235

BLAST of Sed0022633 vs. ExPASy TrEMBL
Match: A0A1S3C9R7 (2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo OX=3656 GN=LOC103498401 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.8e-67
Identity = 139/226 (61.50%), Postives = 164/226 (72.57%), Query Frame = 0

Query: 6   IIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFIPCS 65
           +  +  PFLR+Y+DGRV+RL ITSD VPA ADDP S FRSKDVTI  DPAVSARVFIP S
Sbjct: 11  VAFDLFPFLRVYSDGRVQRLMITSDFVPADADDPKSPFRSKDVTISTDPAVSARVFIPSS 70

Query: 66  AEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIPACY 125
           A+P Q+LP+L++VHGGAFC  SAFNL +H HVG+L+ +ANAVAV+VEYRLAPEHPIPACY
Sbjct: 71  ADPNQKLPLLLYVHGGAFCIESAFNLQYHQHVGALAAEANAVAVSVEYRLAPEHPIPACY 130

Query: 126 DDCWDALRWVAAHVN-SILEGWSRTMVEGACRLQPCDLSRGQR--HHVAARAGSSPDHLG 185
           DDCWDALRWVAAHVN   LE W  T V+   R+     S G    H++AARA SS + LG
Sbjct: 131 DDCWDALRWVAAHVNRDGLEPWLNTYVD-FNRICLAGDSAGANICHYLAARASSSSEELG 190

Query: 186 GVKVLAMALIHVFFGDGGRRQ-----AVETSLLRTPKPSASARGAR 224
           G KV+AMALIH FFGDG   +       ET L+R  K   +  G +
Sbjct: 191 GAKVVAMALIHPFFGDGREDRLWKYLCSETRLVRPTKEELAKMGCK 235

BLAST of Sed0022633 vs. ExPASy TrEMBL
Match: A0A6J1J7Y5 (probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484278 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 3.4e-63
Identity = 124/198 (62.63%), Postives = 150/198 (75.76%), Query Frame = 0

Query: 6   IIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFIPCS 65
           +  EF PFLRIY +GR++RLQ+TSD VPA ADDPNS FR+KDVTI  D A+S R+FIP +
Sbjct: 8   VADEFFPFLRIYKNGRIQRLQVTSDIVPACADDPNSAFRAKDVTISVDSALSVRIFIPSA 67

Query: 66  AEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIPACY 125
            +  Q+LP+L++VHGGAFCF SAFNL +H HVGSL+ KANAVAV+VEYRLAPE+PIPACY
Sbjct: 68  VDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENPIPACY 127

Query: 126 DDCWDALRWVAAHVNSILEGWSRTMVEGACRLQPC----DLSRGQRHHVAARAGSSPDHL 185
           DDCWDALRW+AAHVN   +G    + E A   + C           HH+A RAGSS + L
Sbjct: 128 DDCWDALRWIAAHVNR--DGPEPWLNEHADLDRICLAGDSAGANICHHLAVRAGSSAEQL 187

Query: 186 GGVKVLAMALIHVFFGDG 200
           G VKV+A+ALIH FFGDG
Sbjct: 188 GRVKVVALALIHPFFGDG 203

BLAST of Sed0022633 vs. ExPASy TrEMBL
Match: A0A6J1FTL3 (probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111448682 PE=4 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 2.2e-62
Identity = 124/197 (62.94%), Postives = 149/197 (75.63%), Query Frame = 0

Query: 6   IIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFIPCS 65
           +  EF PFLRIY DGR++RLQ+TSD V A ADDPNS FR+KDVTI  D A+S R+FIP +
Sbjct: 8   VADEFFPFLRIYKDGRIQRLQVTSDIVSACADDPNSAFRAKDVTISVDSALSVRIFIPSA 67

Query: 66  AEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIPACY 125
            +  Q+LP+L++VHGGAFCF SAFNL +H HVGSL+ KANAVAV+VEYRLAPE+PIPACY
Sbjct: 68  VDLNQKLPLLLYVHGGAFCFESAFNLQYHRHVGSLAAKANAVAVSVEYRLAPENPIPACY 127

Query: 126 DDCWDALRWVAAHVN-SILEGWSRTMVEGACRLQPCDLSRGQR--HHVAARAGSSPDHLG 185
           DDCWDALRW+AAHVN    E W     +   R+     S G    HH+A RAG+S + LG
Sbjct: 128 DDCWDALRWIAAHVNRDGPEPWLNQHAD-LDRICLAGDSAGANICHHLAVRAGTSAEQLG 187

Query: 186 GVKVLAMALIHVFFGDG 200
            VKV+A+ALIH FFGDG
Sbjct: 188 RVKVVALALIHPFFGDG 203

BLAST of Sed0022633 vs. TAIR 10
Match: AT1G49660.1 (carboxyesterase 5 )

HSP 1 Score: 129.8 bits (325), Expect = 2.9e-30
Identity = 76/196 (38.78%), Postives = 112/196 (57.14%), Query Frame = 0

Query: 6   IIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFIP-- 65
           I  EFLPF RIY DGRVERL I +DT+PA   DP     SKDV    +  +S R+F+P  
Sbjct: 5   IASEFLPFCRIYKDGRVERL-IGTDTIPASL-DPTYDVVSKDVIYSPENNLSVRLFLPHK 64

Query: 66  -CSAEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIP 125
                 G +LP+L+++HGGA+   S F+ ++H ++  +   AN +AV+V+YR APE P+P
Sbjct: 65  STKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVP 124

Query: 126 ACYDDCWDALRWVAAHVN-SILEGWSRTMVEGACRLQPCDLSRGQ-RHHVAARAGSSPDH 185
           A Y+D W A++W+ AH N S    W     +        D + G   HH+A +AG   + 
Sbjct: 125 AAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGK--EK 184

Query: 186 LGGVKVLAMALIHVFF 197
              +K+  +A++H  F
Sbjct: 185 KLDLKIKGIAVVHPAF 196

BLAST of Sed0022633 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 125.6 bits (314), Expect = 5.4e-29
Identity = 71/194 (36.60%), Postives = 108/194 (55.67%), Query Frame = 0

Query: 4   KTIIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARVFIP 63
           K +  E LP+L ++ DG VERL  T +  P G  DP +G  SKD+ I+    +SAR++ P
Sbjct: 6   KQVSLELLPWLVVHTDGTVERLAGT-EVCPPGL-DPITGVFSKDIIIEPKTGLSARIYRP 65

Query: 64  CSAEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPEHPIPA 123
            S +PGQ++P++++ HGGAF   S     +H  +  +  +AN +AV+V YRLAPEHP+P 
Sbjct: 66  FSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLPT 125

Query: 124 CYDDCWDALRWVAAHVNSILEGWSRTMVE-GACRLQPCDLSRGQRHHVAARAGSSPDHLG 183
            Y+D W AL+    ++ +I E W     +  +  L          HH+A RA  S   L 
Sbjct: 126 AYEDSWTALK----NIQAINEPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSDQTL- 185

Query: 184 GVKVLAMALIHVFF 197
             K+  + +IH +F
Sbjct: 186 --KIKGIGMIHPYF 190

BLAST of Sed0022633 vs. TAIR 10
Match: AT1G49640.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 120.9 bits (302), Expect = 1.3e-27
Identity = 62/182 (34.07%), Postives = 104/182 (57.14%), Query Frame = 0

Query: 1   MDSKTIIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARV 60
           M+S       LPF+RI+ +GRVERL  + + +   + +P +   SKDV   +D  +S R+
Sbjct: 1   MESDLTTEHHLPFIRIHKNGRVERL--SGNDIKPTSLNPQNDVVSKDVMYSSDHNLSVRM 60

Query: 61  FIPCSAE----PGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLA 120
           F+P  +      G ++P+L++ HGGA+   S F+ ++H ++  + + AN +AV+V+YRLA
Sbjct: 61  FLPNKSRKLDTAGNKIPLLIYFHGGAYIIQSPFSPVYHNYLTEVVITANCLAVSVQYRLA 120

Query: 121 PEHPIPACYDDCWDALRWVAAHVNSILEGWS---RTMVEGACRLQPCDLSRGQRHHVAAR 176
           PEHP+PA YDD W A++W+ +H +  +  ++   R  + G              HH+  R
Sbjct: 121 PEHPVPAAYDDSWSAIQWIFSHSDDWINEYADFDRVFIAGD------SAGANISHHMGIR 174

BLAST of Sed0022633 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 119.8 bits (299), Expect = 3.0e-27
Identity = 73/206 (35.44%), Postives = 110/206 (53.40%), Query Frame = 0

Query: 1   MDSKTIIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARV 60
           MDS+ I  ++ P L IY  GR+ERL +   TVP  + +P +G  SKDV    D  +S R+
Sbjct: 1   MDSE-IAADYSPMLIIYKSGRIERL-VGETTVPP-SSNPQNGVVSKDVVYSPDNNLSLRI 60

Query: 61  FIP-----CSAEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRL 120
           ++P        E   +LP+LV+ HGG F   +AF+  +H  + +    ++ VAV+V+YR 
Sbjct: 61  YLPEKAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRR 120

Query: 121 APEHPIPACYDDCWDALRWVAAHV-NSILEGWSRTMVEGACRLQPCDLSRGQ-RHHVAAR 180
           APEHPIP  YDD W AL+WV +H+  S  E W     + +      D +     HH+  +
Sbjct: 121 APEHPIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMK 180

Query: 181 AGS---SPDHLGGVKVLAMALIHVFF 197
           A     SP+ L    +  + L+H +F
Sbjct: 181 AAKDKLSPESLNESGISGIILVHPYF 203

BLAST of Sed0022633 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 5.1e-27
Identity = 77/204 (37.75%), Postives = 113/204 (55.39%), Query Frame = 0

Query: 1   MDSKTIIHEFLPFLRIYADGRVERLQITSDTVPAGADDPNSGFRSKDVTIKADPAVSARV 60
           MDS+  + +  P L+IY  GR+ERL +   TVP  + +P +G  SKDV   AD  +S R+
Sbjct: 1   MDSEIAV-DCSPLLKIYKSGRIERL-MGEATVPP-SSEPQNGVVSKDVVYSADNNLSVRI 60

Query: 61  FIP--CSAEPGQRLPVLVFVHGGAFCFGSAFNLMFHCHVGSLSVKANAVAVAVEYRLAPE 120
           ++P   +AE   +LP+LV+ HGG F   +AF+  +H  + +    +N VAV+V+YR APE
Sbjct: 61  YLPEKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPE 120

Query: 121 HPIPACYDDCWDALRWVAAHV-NSILEGWSRTMVEGACRLQPCDLSRGQR--HHVAARAG 180
           HPI   +DD W AL+WV  H+  S  E W     + + R+     S G    HH+A RA 
Sbjct: 121 HPISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFS-RVFLSGDSAGANIVHHMAMRAA 180

Query: 181 S---SPDHLGGVKVLAMALIHVFF 197
               SP  L    +  + L+H +F
Sbjct: 181 KEKLSPG-LNDTGISGIILLHPYF 199