Sed0022534 (gene) Chayote v1

Overview
NameSed0022534
Typegene
OrganismSechium edule (Chayote v1)
Descriptioncytochrome P450 CYP82D47-like
LocationLG13: 22044012 .. 22046517 (+)
RNA-Seq ExpressionSed0022534
SyntenySed0022534
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTAATTACAATATTTATCAAACTTAAGAGAGATAATTAATCAAATTTAAAATATAAAGATATAATTATTACAAAAATCACAATTTATAGGTAGTTCTTGCCTTTTTTCCCATGTCTCTAAAATTTGATTTCTTCTCACGTCTCTAAAATTTTGTGGACATAATGATTTCACAGATCATGTCATAGGGGCCAGCTGAAAAGGCAAAATAATAATATAATAACTTTAATTCAAAATGATTAAATAATAGCAGAAATGTTAACACAATCAGGAGGGCTAAAAATGGAAGAACAACCTCACATTTCTACAACCAACCCCTTCGCCGGAATCTTCGCCGGGATCTTCATTTCTATACGCTCTGTTTTGCGTCTCCCAAAAATCCACCGCAAATCGAAAGAGAAAACCGCCGGAAGCCGGCGGTGGCTTGCCGGTGATCGGGCACCTCCATCTTCTAAGCGCAACAGAGCCAATTCAAACAACATTAGCGAAAATGGCGGACAAATACGGACCCATCTTCACTTTGAAATTGGGGAAAAATAGAGCATTGATCGTAAGCAATTGGGCAATCGCAAAAGAGTGCTTTACAACAAACGACCGAGCCTTCGCCTCGCGCCCAAAGACCGCAGCCGGGAAGCTGCTGGGCTACGACCACGCGTTGATGGGTGTGATCCATTACGGCCCACTTTGGCGCCATGTCCGTAAAACAGTGGCGCTTGAACTTCTCTCGAATCAACGCCTCGATCAGCTCAGCCACATATGGGTGTCTGAAATTGATAATTCGATCAAGAATCTTTATGGGTTTTGCGTCAATTGCAGAAGTGGAACAGTGTTGGTGGAGATGAAGAGCTGATTTGGGGATCTGACGAGGAATATGATGTGTAGGATGGTGGTTGGAGAGCGATTCACGGCGGGGTTGGACGGCGGCGCTGGCGCAGAGTACCGGAATGCGGTGAGGGATTTCTTTGAATTGTTTGGGGCGTTTGTTCCTTCGGATTCGTTTCCGTTCTTGAAGTGGTTTGATTTTGGGGGATATGAAAAGGCGATGAAGAAGACGGCCAAAGTGTTGGATTCGGTGCTTGATTTGTGGATGGAAGATCACCGGAAAAAGAGAAATTCCGGCGAAGTGGAGATGCACGGGAATGACTTCATGGATGTGATGCTCTCTGTTTTTAAGGATGATGGAGGACAAAGATTTGGGTATGATGTTCATACCATCACCAAAGCCACTTGTTTGGTATGCTTTTTTTTTTTTTTTAAATTATGTTAAAGTTTTGAGAAATACTCCCCCTGAACATGAATGCTTAGGTACTCTATTTCTACACATATATGACAATATTAAGTAGGGATGATAATAATAACCAAAAATTGAAAAAGTTGTATGGAACCAAATCTATCCATTCGATTGATTCGAATTTTTCAACTAATATTGGACATATGTGTTTATATTTGGAGAACATCGAAAATTATTGGTTCCGTTCAGTTCCTATTTGTAAAAGTCAATAAAAATGAAACTAAATCAATATTAATTTATTTTTTATTTTGTAGGTTACACTAACTTTTTGTTTGGAAATGACTAATTATTTTTGTTTAATTTAATTATAAATTTTGTTAATTTTTTTAGGAAAAAGCATTCAATAGGAAATAGAAGTAAAATAGTAAAAAATGTGATTGAACATACTACTTTGCCTTTTACTAATGAAAGCGGATCGAAACTATAGGCTATAATATTGGGTGGATGGGACACTACATCAGCAACAATGGAATGGGCTCTTACATTACTCCTCAACGATGAAGAAGCACTAAAAAAAATCCAAATTGAACTAGACGAACAAATTGGGAGAGAAAGAAAAGTGGAAGAGTCAGATGTGAAAAATCTATTATATCTCCAAGCTATAGTGAAGGAAACATTGCGATTGTACCCAGCAGCGCCAATAGCAATTCCACACAAAGCTATAGAAAATTGCACAGTTGCTGGTTACCACATCTCAACAAAGACACAACTAATAGTGAATCTTAAAAAGCTCCAAAGAGATCCACTTATTTGGAAAGATCCTAATGAATTTCGACCAGAAAGATTTTTAACAACTCAAAAGCACTTTAATGTTAGCGGACAAAGTCCCGAATTGATACCATTTGGAAATGGTCGGAAGATGTGCCCTGGAATTTCTTTTGCCCTCCGAATGATGCATCTCACACTAGCAAATCTACTCCATATGTTTGAATTTGGTAGGCCATCACAAGAATTATTGGATATGGAGGAGGGTGTTGGAATTTCTTGCGTTAAAAAAAACCCTCTTCAAATTGTTTTAACTCCACGTCTCCCTATTCGAGTTTATGATTAAATCTTAAGTTTGAATTACAAGTTGAGTTGATGAGCTTTTTAAAGTTTGATTTCATGACATAAATACTATGTTGATAGTTGTGTTTAGTCGTGACATAAATCATTTCTCCCAATTGATGTTGTGGGGAGAATTATCGCTGGTCAAATATTAATTTATTCTAGTGTCA

mRNA sequence

GTTAATTACAATATTTATCAAACTTAAGAGAGATAATTAATCAAATTTAAAATATAAAGATATAATTATTACAAAAATCACAATTTATAGGTAGTTCTTGCCTTTTTTCCCATGTCTCTAAAATTTGATTTCTTCTCACGTCTCTAAAATTTTGTGGACATAATGATTTCACAGATCATGTCATAGGGGCCAGCTGAAAAGGCAAAATAATAATATAATAACTTTAATTCAAAATGATTAAATAATAGCAGAAATGTTAACACAATCAGGAGGGCTAAAAATGGAAGAACAACCTCACATTTCTACAACCAACCCCTTCGCCGGAATCTTCGCCGGGATCTTCATTTCTATACGCTCTGTTTTGCGTCTCCCAAAAATCCACCGCAAATCGAAAGAGAAAACCGCCGGAAGCCGGCGGTGGCTTGCCGGTGATCGGGCACCTCCATCTTCTAAGCGCAACAGAGCCAATTCAAACAACATTAGCGAAAATGGCGGACAAATACGGACCCATCTTCACTTTGAAATTGGGGAAAAATAGAGCATTGATCGTAAGCAATTGGGCAATCGCAAAAGAGTGCTTTACAACAAACGACCGAGCCTTCGCCTCGCGCCCAAAGACCGCAGCCGGGAAGCTGCTGGGCTACGACCACGCGTTGATGGGTGTGATCCATTACGGCCCACTTTGGCGCCATGTCCGTAAAACAGTGGCGCTTGAACTTCTCTCGAATCAACGCCTCGATCAGCTCAGCCACATATGGGTGTCTGAAATTGATAATTCGATCAAGAATCTTTATGGGTTTTGCGTCAATTGCAGAAGTGGAACAGTGTTGGTGGAGATGAAGAGCTGATTTGGGGATCTGACGAGGAATATGATGTGTAGGATGGTGGTTGGAGAGCGATTCACGGCGGGGTTGGACGGCGGCGCTGGCGCAGAGTACCGGAATGCGGTGAGGGATTTCTTTGAATTGTTTGGGGCGTTTGTTCCTTCGGATTCGTTTCCGTTCTTGAAGTGGTTTGATTTTGGGGGATATGAAAAGGCGATGAAGAAGACGGCCAAAGTGTTGGATTCGGTGCTTGATTTGTGGATGGAAGATCACCGGAAAAAGAGAAATTCCGGCGAAGTGGAGATGCACGGGAATGACTTCATGGATGTGATGCTCTCTGTTTTTAAGGATGATGGAGGACAAAGATTTGGGTATGATGTTCATACCATCACCAAAGCCACTTGTTTGGCTATAATATTGGGTGGATGGGACACTACATCAGCAACAATGGAATGGGCTCTTACATTACTCCTCAACGATGAAGAAGCACTAAAAAAAATCCAAATTGAACTAGACGAACAAATTGGGAGAGAAAGAAAAGTGGAAGAGTCAGATGTGAAAAATCTATTATATCTCCAAGCTATAGTGAAGGAAACATTGCGATTGTACCCAGCAGCGCCAATAGCAATTCCACACAAAGCTATAGAAAATTGCACAGTTGCTGGTTACCACATCTCAACAAAGACACAACTAATAGTGAATCTTAAAAAGCTCCAAAGAGATCCACTTATTTGGAAAGATCCTAATGAATTTCGACCAGAAAGATTTTTAACAACTCAAAAGCACTTTAATGTTAGCGGACAAAGTCCCGAATTGATACCATTTGGAAATGGTCGGAAGATGTGCCCTGGAATTTCTTTTGCCCTCCGAATGATGCATCTCACACTAGCAAATCTACTCCATATGTTTGAATTTGGTAGGCCATCACAAGAATTATTGGATATGGAGGAGGGTGTTGGAATTTCTTGCGTTAAAAAAAACCCTCTTCAAATTGTTTTAACTCCACGTCTCCCTATTCGAGTTTATGATTAAATCTTAAGTTTGAATTACAAGTTGAGTTGATGAGCTTTTTAAAGTTTGATTTCATGACATAAATACTATGTTGATAGTTGTGTTTAGTCGTGACATAAATCATTTCTCCCAATTGATGTTGTGGGGAGAATTATCGCTGGTCAAATATTAATTTATTCTAGTGTCA

Coding sequence (CDS)

ATGATGTGTAGGATGGTGGTTGGAGAGCGATTCACGGCGGGGTTGGACGGCGGCGCTGGCGCAGAGTACCGGAATGCGGTGAGGGATTTCTTTGAATTGTTTGGGGCGTTTGTTCCTTCGGATTCGTTTCCGTTCTTGAAGTGGTTTGATTTTGGGGGATATGAAAAGGCGATGAAGAAGACGGCCAAAGTGTTGGATTCGGTGCTTGATTTGTGGATGGAAGATCACCGGAAAAAGAGAAATTCCGGCGAAGTGGAGATGCACGGGAATGACTTCATGGATGTGATGCTCTCTGTTTTTAAGGATGATGGAGGACAAAGATTTGGGTATGATGTTCATACCATCACCAAAGCCACTTGTTTGGCTATAATATTGGGTGGATGGGACACTACATCAGCAACAATGGAATGGGCTCTTACATTACTCCTCAACGATGAAGAAGCACTAAAAAAAATCCAAATTGAACTAGACGAACAAATTGGGAGAGAAAGAAAAGTGGAAGAGTCAGATGTGAAAAATCTATTATATCTCCAAGCTATAGTGAAGGAAACATTGCGATTGTACCCAGCAGCGCCAATAGCAATTCCACACAAAGCTATAGAAAATTGCACAGTTGCTGGTTACCACATCTCAACAAAGACACAACTAATAGTGAATCTTAAAAAGCTCCAAAGAGATCCACTTATTTGGAAAGATCCTAATGAATTTCGACCAGAAAGATTTTTAACAACTCAAAAGCACTTTAATGTTAGCGGACAAAGTCCCGAATTGATACCATTTGGAAATGGTCGGAAGATGTGCCCTGGAATTTCTTTTGCCCTCCGAATGATGCATCTCACACTAGCAAATCTACTCCATATGTTTGAATTTGGTAGGCCATCACAAGAATTATTGGATATGGAGGAGGGTGTTGGAATTTCTTGCGTTAAAAAAAACCCTCTTCAAATTGTTTTAACTCCACGTCTCCCTATTCGAGTTTATGATTAA

Protein sequence

MMCRMVVGERFTAGLDGGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYEKAMKKTAKVLDSVLDLWMEDHRKKRNSGEVEMHGNDFMDVMLSVFKDDGGQRFGYDVHTITKATCLAIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLLYLQAIVKETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNEFRPERFLTTQKHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQELLDMEEGVGISCVKKNPLQIVLTPRLPIRVYD
Homology
BLAST of Sed0022534 vs. NCBI nr
Match: XP_022935739.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 455.7 bits (1171), Expect = 3.4e-124
Identity = 223/327 (68.20%), Postives = 269/327 (82.26%), Query Frame = 0

Query: 4   RMVVGERFTAGLDGGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYEKAMKKTAK 63
           R+VVG+RF+  ++G    EYR A+RDFFE FG FVPSDSFPFLKW D GG+EKAMK TAK
Sbjct: 204 RIVVGKRFSTAVEGSGNEEYRTALRDFFEWFGVFVPSDSFPFLKWLDLGGHEKAMKNTAK 263

Query: 64  VLDSVLDLWMEDHR-KKRNSGEVEMHG-NDFMDVMLSVFKDDGGQRFGYDVHTITKATCL 123
           VLD V D W+++HR KK+N GE +M    DFMDVMLS  KDD GQ   YD HT+TKATCL
Sbjct: 264 VLDEVFDKWLQEHRQKKKNLGEGKMEDPPDFMDVMLSNVKDD-GQLSNYDAHTVTKATCL 323

Query: 124 AIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLLYLQAIV 183
           A+IL G DTT+ TM WAL+LLLN  E +++ Q+ELDEQ+GR+R+V E DVKNLLYLQAIV
Sbjct: 324 ALILAGSDTTTITMVWALSLLLNHPEVIERAQLELDEQVGRQRQVREFDVKNLLYLQAIV 383

Query: 184 KETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNEFRPERF 243
           KETLRLYPAAPI IPH++IE+CTVAGYHI   T+LIVN++KLQRDP IWKDP EFRPERF
Sbjct: 384 KETLRLYPAAPILIPHESIEDCTVAGYHIPAGTRLIVNVQKLQRDPQIWKDPCEFRPERF 443

Query: 244 LTTQKHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQELLDME 303
           LT  K F+V GQSP+LIPFG+GR+MCPGISFAL+++HLTLANLLH FE  RPS++LLDME
Sbjct: 444 LTDNKDFDVRGQSPQLIPFGSGRRMCPGISFALQVLHLTLANLLHGFEIRRPSKQLLDME 503

Query: 304 EGVGISCVKKNPLQIVLTPRLPIRVYD 329
           E  G++ ++KN L+ V+TPRLP +VY+
Sbjct: 504 ESAGMTSIRKNLLETVITPRLPAQVYE 529

BLAST of Sed0022534 vs. NCBI nr
Match: XP_008464765.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo] >KAA0032725.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa] >TYJ98756.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa])

HSP 1 Score: 454.5 bits (1168), Expect = 7.6e-124
Identity = 219/327 (66.97%), Postives = 270/327 (82.57%), Query Frame = 0

Query: 4   RMVVGERFTAGLD--GGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYEKAMKKT 63
           R+VVG++F+  +D       EYR A+RDFFE FG FVPSDSFPFLKW D GG+EKAMKKT
Sbjct: 200 RIVVGKKFSTAVDASNNENEEYRKALRDFFEWFGVFVPSDSFPFLKWLDLGGHEKAMKKT 259

Query: 64  AKVLDSVLDLWMEDHRKKRNSGEVEMHGNDFMDVMLSVFKDDGGQRFGYDVHTITKATCL 123
           AKVLD V D W++DH  K N GEV+M  +DFMDVMLS  +DD GQ   YD HT+TKATCL
Sbjct: 260 AKVLDEVFDKWIQDH--KNNLGEVKMEEHDFMDVMLSNVRDD-GQLSKYDAHTVTKATCL 319

Query: 124 AIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLLYLQAIV 183
           ++IL G DTT+ TM WAL+LLLN++E LK+ Q+ELDE++GR+R+V+ESDVKNLLYLQAIV
Sbjct: 320 SLILAGSDTTTVTMIWALSLLLNNQEVLKRAQLELDERVGRQRQVKESDVKNLLYLQAIV 379

Query: 184 KETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNEFRPERF 243
           KETLRLYPAAPI +PH+++++C VAGYHI   T+LIVN++KLQRDP IW+DP EFRPERF
Sbjct: 380 KETLRLYPAAPILVPHESVDDCVVAGYHIPVGTRLIVNVQKLQRDPQIWEDPCEFRPERF 439

Query: 244 LTTQKHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQELLDME 303
           LT+ K F+V GQ+P LIPFG+GR+MCPGISFAL++MHL LANLLH FE  RPS+E LDME
Sbjct: 440 LTSAKDFDVRGQNPRLIPFGSGRRMCPGISFALQVMHLALANLLHGFEISRPSKEFLDME 499

Query: 304 EGVGISCVKKNPLQIVLTPRLPIRVYD 329
           E  G++ ++KNPL++VLTPRLP  VY+
Sbjct: 500 ESAGMTSIRKNPLEVVLTPRLPPHVYE 523

BLAST of Sed0022534 vs. NCBI nr
Match: XP_023535456.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 452.6 bits (1163), Expect = 2.9e-123
Identity = 219/326 (67.18%), Postives = 266/326 (81.60%), Query Frame = 0

Query: 4   RMVVGERFTAGLDGGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYEKAMKKTAK 63
           R+VVG+RF+  ++G    EYR A+RDFFE FG FVPSDSFPFLKW D GG+EKAMK TAK
Sbjct: 204 RIVVGKRFSTAVEGSGNEEYRTALRDFFEWFGVFVPSDSFPFLKWLDLGGHEKAMKNTAK 263

Query: 64  VLDSVLDLWMEDHRKKRNSGEVEM-HGNDFMDVMLSVFKDDGGQRFGYDVHTITKATCLA 123
           VLD V D W+++HR+ +N  E +M   + FMDVMLS  KDD GQ   YD HT+TKATCLA
Sbjct: 264 VLDEVFDKWLQEHRQNKNVSEGKMEEPHHFMDVMLSNVKDD-GQLSNYDAHTVTKATCLA 323

Query: 124 IILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLLYLQAIVK 183
           +IL G DTT+ TM WAL+LLLN  E +++ Q+ELDE +GR+R+V ESDVKNLLYLQAIVK
Sbjct: 324 LILAGSDTTTITMVWALSLLLNHPEVIERAQLELDEHVGRQRQVRESDVKNLLYLQAIVK 383

Query: 184 ETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNEFRPERFL 243
           ETLRLYPAAPI IPH++IE+CTV GYHI   T+LIVN++KLQRDP IWKDP EFRPERFL
Sbjct: 384 ETLRLYPAAPILIPHESIEDCTVVGYHIPAGTRLIVNVQKLQRDPQIWKDPCEFRPERFL 443

Query: 244 TTQKHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQELLDMEE 303
           T  K F+V GQSP+LIPFG+GR+MCPGISFAL+++HLTLANLLH FE  RPS+ELLDMEE
Sbjct: 444 TDNKDFDVRGQSPQLIPFGSGRRMCPGISFALQVLHLTLANLLHGFEIRRPSKELLDMEE 503

Query: 304 GVGISCVKKNPLQIVLTPRLPIRVYD 329
             G++ ++KN L+ V+TPRLP +VY+
Sbjct: 504 SAGMTSIRKNLLEAVITPRLPAQVYE 528

BLAST of Sed0022534 vs. NCBI nr
Match: XP_004147803.2 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59914.1 hypothetical protein Csa_001231 [Cucumis sativus])

HSP 1 Score: 452.2 bits (1162), Expect = 3.8e-123
Identity = 218/329 (66.26%), Postives = 274/329 (83.28%), Query Frame = 0

Query: 4   RMVVGERFTAGLD---GGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYEKAMKK 63
           R+VVG++F+  +D        EYR A+RDFFE FG FVPSDSFPFLKW D GG+EKAMKK
Sbjct: 210 RIVVGKQFSTAVDVSNTNENEEYRKALRDFFEWFGVFVPSDSFPFLKWLDLGGHEKAMKK 269

Query: 64  TAKVLDSVLDLWMEDHRKKRNS-GEVEMHGNDFMDVMLSVFKDDGGQRFGYDVHTITKAT 123
           TA+VLD V D W+++H++K+N+ G V+M  +DFMDVMLS  +DD GQ   YD H +TKAT
Sbjct: 270 TAQVLDEVFDKWIQEHQQKKNNLGAVKMEEHDFMDVMLSNVRDD-GQLSKYDAHIVTKAT 329

Query: 124 CLAIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLLYLQA 183
           CLA+IL G DTT+ TM WAL+LLLN++E LK+ Q+ELDE +GR+R+V+ESDVKNLLYLQA
Sbjct: 330 CLALILAGSDTTTVTMIWALSLLLNNQEVLKRAQLELDEHVGRQRQVKESDVKNLLYLQA 389

Query: 184 IVKETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNEFRPE 243
           +VKETLRLYPAAPI IPH++IE+C VAGYHI   T+LIVN++KLQRDP IW+DP EF PE
Sbjct: 390 VVKETLRLYPAAPILIPHESIEDCVVAGYHIPLGTRLIVNVQKLQRDPQIWEDPCEFHPE 449

Query: 244 RFLTTQKHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQELLD 303
           RFLT++K F+V GQSP+LIPFG+GR+MCPGISFAL++MHL LANLLH FE  RP++ELLD
Sbjct: 450 RFLTSEKDFDVRGQSPQLIPFGSGRRMCPGISFALQVMHLALANLLHGFEISRPTKELLD 509

Query: 304 MEEGVGISCVKKNPLQIVLTPRLPIRVYD 329
           MEE  G++ ++KNPL++VLTPRLP +VY+
Sbjct: 510 MEESAGMTSIRKNPLEVVLTPRLPPQVYE 537

BLAST of Sed0022534 vs. NCBI nr
Match: XP_038899514.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 449.9 bits (1156), Expect = 1.9e-122
Identity = 211/326 (64.72%), Postives = 266/326 (81.60%), Query Frame = 0

Query: 4   RMVVGERFTAGLDGGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYEKAMKKTAK 63
           RMV+GE FT   +GG G  Y+ A+RDFF+LFG FVP DSFPFL+W D GG+ +AMKKTAK
Sbjct: 201 RMVIGEGFTRAFEGGDGERYKKAMRDFFDLFGVFVPGDSFPFLRWLDLGGHVRAMKKTAK 260

Query: 64  VLDSVLDLWMEDHRKKRNSGEVEMHGN-DFMDVMLSVFKDDGGQRFGYDVHTITKATCLA 123
           ++D +L+ W+++HR+K N  ++      DFMDVMLS+ KDD       D   +TKATCL 
Sbjct: 261 IMDEMLNKWLKEHREKGNFIDLARAKEFDFMDVMLSLVKDDAELSRYDDDDRVTKATCLG 320

Query: 124 IILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLLYLQAIVK 183
           +ILGG+DTT+  M+WAL+LLLN+ + LKK+Q+EL EQ+G+ERKVEESDVKNLLYLQA+VK
Sbjct: 321 MILGGFDTTAIAMQWALSLLLNNPDTLKKLQVELHEQVGQERKVEESDVKNLLYLQAVVK 380

Query: 184 ETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNEFRPERFL 243
           ETLRLYPA PIA+PH++IENC VA YHIST+T+L+VN +KLQRDPL+WKDPNEFRPERFL
Sbjct: 381 ETLRLYPAVPIALPHESIENCIVADYHISTQTRLLVNRQKLQRDPLVWKDPNEFRPERFL 440

Query: 244 TTQKHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQELLDMEE 303
           + + HF+V GQ+P+L+PFG+GR MCPGISFAL+ + LTLANLLHMFE  RPS EL+DME 
Sbjct: 441 SRENHFDVRGQNPQLVPFGSGRMMCPGISFALQFVSLTLANLLHMFEVSRPSNELIDMEM 500

Query: 304 GVGISCVKKNPLQIVLTPRLPIRVYD 329
           GVG +C++KN L+IVL PRLP  VY+
Sbjct: 501 GVGTTCIRKNSLEIVLNPRLPAEVYE 526

BLAST of Sed0022534 vs. ExPASy Swiss-Prot
Match: O49396 (Cytochrome P450 82C3 OS=Arabidopsis thaliana OX=3702 GN=CYP82C3 PE=2 SV=3)

HSP 1 Score: 323.6 bits (828), Expect = 2.7e-87
Identity = 160/331 (48.34%), Postives = 230/331 (69.49%), Query Frame = 0

Query: 1   MMCRMVVGERFTAGLDGGA--------GAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFG 60
           M+ RMV G+R+  G  GGA          ++R  +  FF L G F  SD+FP L W D  
Sbjct: 178 MIMRMVAGKRYFGG--GGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDLQ 237

Query: 61  GYEKAMKKTAKVLDSVLDLWMEDHRKKRNSGEVEMHGNDFMDVMLSVFKDDGGQRFGYDV 120
           G+EK MK+T + LD +L+ W+E+HR++R     + + +DF+DVMLS+ +        YD 
Sbjct: 238 GHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKLSHLQYDA 297

Query: 121 HTITKATCLAIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVK 180
           +T  K TCLA+ILGG +T+ +T+ WA++LLLN+++ LKK+Q E+D  +GR+R VE+SD+K
Sbjct: 298 NTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIK 357

Query: 181 NLLYLQAIVKETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKD 240
           NL+YLQAI+KETLRLYPAAP+    +A+E+CTVAGY++   T+LIVN+ K+QRDP ++ +
Sbjct: 358 NLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYME 417

Query: 241 PNEFRPERFLTTQ-KHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFG 300
           PNEFRPERF+T + K F+V GQ+ EL+PFG+GR+ CPG S A++M+HL LA  LH FE  
Sbjct: 418 PNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVK 477

Query: 301 RPSQELLDMEEGVGISCVKKNPLQIVLTPRL 323
                 +DM E  G++  K  PL++++ PRL
Sbjct: 478 TVLDRPVDMSESPGLTITKATPLEVLINPRL 506

BLAST of Sed0022534 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 7.8e-87
Identity = 163/327 (49.85%), Postives = 221/327 (67.58%), Query Frame = 0

Query: 1   MMCRMVVGERFTAGLDGGAGAEYRNA-----VRDFFELFGAFVPSDSFPFLKWFDFGGYE 60
           M  +MVVG+RF       A  E  ++     +R+ F L G+FV SD  P+L+W D GG+E
Sbjct: 192 MSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYLRWLDLGGHE 251

Query: 61  KAMKKTAKVLDSVLDLWMEDHRKKRNSGEVEMHGNDFMDVMLSVFKDDGGQRFGYDVHTI 120
           K MK+T K LD +   W+++H++KR SG  E    DFMDVMLS+ ++    + G DV TI
Sbjct: 252 KEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQDFMDVMLSILEE---SKLGNDVDTI 311

Query: 121 TKATCLAIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLL 180
            K  CLAIILGG DTT AT+ WAL+LLLN+  ALKK Q ELD  +GR+R V+ESD+  L 
Sbjct: 312 NKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDRNVDESDLVKLT 371

Query: 181 YLQAIVKETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNE 240
           Y+ AI+KETLRLYP  P+  P    E+CT+AGYH+   T+LIVN  K+QRDPL+W  P+E
Sbjct: 372 YIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDPLVWSQPHE 431

Query: 241 FRPERFLTTQKHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQ 300
           ++PERFL  ++  ++ GQ  ELIPFG+GR+ CP IS AL+++ LTLA++LH FE   P+Q
Sbjct: 432 YQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHILHGFELRTPNQ 491

Query: 301 ELLDMEEGVGISCVKKNPLQIVLTPRL 323
             +DM E  GI   K  PL++++ PR+
Sbjct: 492 NKVDMTETPGIVHAKATPLEVLVAPRI 513

BLAST of Sed0022534 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 5.0e-86
Identity = 155/329 (47.11%), Postives = 230/329 (69.91%), Query Frame = 0

Query: 1   MMCRMVVGERFTAG------LDGGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGY 60
           M+ RMV G+R+  G       D     + + A+  FF L G F  SD+FP L +FD  G+
Sbjct: 190 MIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGH 249

Query: 61  EKAMKKTAKVLDSVLDLWMEDHRKKRNSGEVEMHGNDFMDVMLSVFKDDGGQRFGYDVHT 120
           EK MK+T   LD +L+ W+E+HR++R     + + +DF+DVM+S+ +        YD +T
Sbjct: 250 EKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHLQYDANT 309

Query: 121 ITKATCLAIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNL 180
             K+TCLA+ILGG DT+++T+ WA++LLLN++E LKK Q E+D  +GR+R VE+SD++NL
Sbjct: 310 SIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENL 369

Query: 181 LYLQAIVKETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPN 240
           +YLQAI+KETLRLYPA P+  P +A+E+CTVAGY++   T+LIVN+ K+QRDP ++ +PN
Sbjct: 370 VYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPN 429

Query: 241 EFRPERFLTTQ-KHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRP 300
           EFRPERF+T + K F+V GQ+ EL+PFG+GR+ CPG S A++++HL LA  LH F+    
Sbjct: 430 EFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTV 489

Query: 301 SQELLDMEEGVGISCVKKNPLQIVLTPRL 323
               +DM E  G++  K  PL+++++PR+
Sbjct: 490 MDMPVDMSENPGLTIPKATPLEVLISPRI 518

BLAST of Sed0022534 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 315.8 bits (808), Expect = 5.6e-85
Identity = 156/333 (46.85%), Postives = 230/333 (69.07%), Query Frame = 0

Query: 1   MMCRMVVGERFTAG-----LDGGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYE 60
           MM RMV G+R+  G      D     + R  V +FF L G F  SD+FP L WFDF G+E
Sbjct: 190 MMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGHE 249

Query: 61  KAMKKTAKVLDSVLDLWMEDHRKKRNSGEVEMHGNDFMDVMLSVFKDDGGQRFGYDVHTI 120
           K MK+T + LD +L+ W+E+HR++R     + + +DF+DVMLS+ +        +D  T 
Sbjct: 250 KEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHLQHDAITS 309

Query: 121 TKATCLAIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLL 180
            K+TCLA+ILGG +T+ +T+ WA++LLLN+++ LKK Q E+D  +GR+R VE+SD++NL+
Sbjct: 310 IKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLV 369

Query: 181 YLQAIVKETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNE 240
           Y+QAI+KETLRLYPA P+    +AIE+CTVAGY++   T+++VN+ K+QRDP ++ +PNE
Sbjct: 370 YIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNE 429

Query: 241 FRPERFLTTQ-KHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPS 300
           FRPERF+T + K F+V GQ+ EL+PFG+GR+ CPG S A++++HL LA  L  F+     
Sbjct: 430 FRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVM 489

Query: 301 QELLDMEEGVGISCVKKNPLQIVLTPRLPIRVY 328
              +DM E  G++  K  PL+I+++PRL   +Y
Sbjct: 490 DMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522

BLAST of Sed0022534 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 4.7e-84
Identity = 160/331 (48.34%), Postives = 217/331 (65.56%), Query Frame = 0

Query: 1   MMCRMVVGERF--TAGLDGGAGAE-YRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYEKA 60
           M+ RMVVG+R+     ++G   A+ +   +R+F  L G F  +D  P L+W D GG+EKA
Sbjct: 197 MVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPCLRWLDLGGHEKA 256

Query: 61  MKKTAKVLDSVLDLWMEDHRKKRNSGEVEMHGNDFMDVMLSVFKDDGGQRFGYDVHTITK 120
           MK  AK +D +L  W+E+HR+K+  GE      DFMDVM+S    +G Q   +D  TI K
Sbjct: 257 MKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISAL--NGAQIGAFDADTICK 316

Query: 121 ATCLAIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLLYL 180
           AT L +ILGG D+T+ T+ WAL+LLL +  AL K + E+D QIG++  + ESD+  L+YL
Sbjct: 317 ATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYIRESDISKLVYL 376

Query: 181 QAIVKETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNEFR 240
           QAIVKETLRLYP AP + P +  ENC + GYHI   T+LI NL K+ RDP +W DP EF+
Sbjct: 377 QAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRDPSVWSDPLEFK 436

Query: 241 PERFLTTQKHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQEL 300
           PERFLTT K  ++ G + EL+PFG+GR++C G+S  L M+H TLANLLH F+   PS E 
Sbjct: 437 PERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLLHSFDILNPSAEP 496

Query: 301 LDMEEGVGISCVKKNPLQIVLTPRLPIRVYD 329
           +DM E  G +  K  PL+I++ PR     Y+
Sbjct: 497 VDMTEFFGFTNTKATPLEILVKPRQSPNYYE 525

BLAST of Sed0022534 vs. ExPASy TrEMBL
Match: A0A6J1F697 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442562 PE=3 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.7e-124
Identity = 223/327 (68.20%), Postives = 269/327 (82.26%), Query Frame = 0

Query: 4   RMVVGERFTAGLDGGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYEKAMKKTAK 63
           R+VVG+RF+  ++G    EYR A+RDFFE FG FVPSDSFPFLKW D GG+EKAMK TAK
Sbjct: 204 RIVVGKRFSTAVEGSGNEEYRTALRDFFEWFGVFVPSDSFPFLKWLDLGGHEKAMKNTAK 263

Query: 64  VLDSVLDLWMEDHR-KKRNSGEVEMHG-NDFMDVMLSVFKDDGGQRFGYDVHTITKATCL 123
           VLD V D W+++HR KK+N GE +M    DFMDVMLS  KDD GQ   YD HT+TKATCL
Sbjct: 264 VLDEVFDKWLQEHRQKKKNLGEGKMEDPPDFMDVMLSNVKDD-GQLSNYDAHTVTKATCL 323

Query: 124 AIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLLYLQAIV 183
           A+IL G DTT+ TM WAL+LLLN  E +++ Q+ELDEQ+GR+R+V E DVKNLLYLQAIV
Sbjct: 324 ALILAGSDTTTITMVWALSLLLNHPEVIERAQLELDEQVGRQRQVREFDVKNLLYLQAIV 383

Query: 184 KETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNEFRPERF 243
           KETLRLYPAAPI IPH++IE+CTVAGYHI   T+LIVN++KLQRDP IWKDP EFRPERF
Sbjct: 384 KETLRLYPAAPILIPHESIEDCTVAGYHIPAGTRLIVNVQKLQRDPQIWKDPCEFRPERF 443

Query: 244 LTTQKHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQELLDME 303
           LT  K F+V GQSP+LIPFG+GR+MCPGISFAL+++HLTLANLLH FE  RPS++LLDME
Sbjct: 444 LTDNKDFDVRGQSPQLIPFGSGRRMCPGISFALQVLHLTLANLLHGFEIRRPSKQLLDME 503

Query: 304 EGVGISCVKKNPLQIVLTPRLPIRVYD 329
           E  G++ ++KN L+ V+TPRLP +VY+
Sbjct: 504 ESAGMTSIRKNLLETVITPRLPAQVYE 529

BLAST of Sed0022534 vs. ExPASy TrEMBL
Match: A0A5A7SU10 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G00630 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.7e-124
Identity = 219/327 (66.97%), Postives = 270/327 (82.57%), Query Frame = 0

Query: 4   RMVVGERFTAGLD--GGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYEKAMKKT 63
           R+VVG++F+  +D       EYR A+RDFFE FG FVPSDSFPFLKW D GG+EKAMKKT
Sbjct: 200 RIVVGKKFSTAVDASNNENEEYRKALRDFFEWFGVFVPSDSFPFLKWLDLGGHEKAMKKT 259

Query: 64  AKVLDSVLDLWMEDHRKKRNSGEVEMHGNDFMDVMLSVFKDDGGQRFGYDVHTITKATCL 123
           AKVLD V D W++DH  K N GEV+M  +DFMDVMLS  +DD GQ   YD HT+TKATCL
Sbjct: 260 AKVLDEVFDKWIQDH--KNNLGEVKMEEHDFMDVMLSNVRDD-GQLSKYDAHTVTKATCL 319

Query: 124 AIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLLYLQAIV 183
           ++IL G DTT+ TM WAL+LLLN++E LK+ Q+ELDE++GR+R+V+ESDVKNLLYLQAIV
Sbjct: 320 SLILAGSDTTTVTMIWALSLLLNNQEVLKRAQLELDERVGRQRQVKESDVKNLLYLQAIV 379

Query: 184 KETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNEFRPERF 243
           KETLRLYPAAPI +PH+++++C VAGYHI   T+LIVN++KLQRDP IW+DP EFRPERF
Sbjct: 380 KETLRLYPAAPILVPHESVDDCVVAGYHIPVGTRLIVNVQKLQRDPQIWEDPCEFRPERF 439

Query: 244 LTTQKHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQELLDME 303
           LT+ K F+V GQ+P LIPFG+GR+MCPGISFAL++MHL LANLLH FE  RPS+E LDME
Sbjct: 440 LTSAKDFDVRGQNPRLIPFGSGRRMCPGISFALQVMHLALANLLHGFEISRPSKEFLDME 499

Query: 304 EGVGISCVKKNPLQIVLTPRLPIRVYD 329
           E  G++ ++KNPL++VLTPRLP  VY+
Sbjct: 500 ESAGMTSIRKNPLEVVLTPRLPPHVYE 523

BLAST of Sed0022534 vs. ExPASy TrEMBL
Match: A0A1S3CM78 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502573 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.7e-124
Identity = 219/327 (66.97%), Postives = 270/327 (82.57%), Query Frame = 0

Query: 4   RMVVGERFTAGLD--GGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYEKAMKKT 63
           R+VVG++F+  +D       EYR A+RDFFE FG FVPSDSFPFLKW D GG+EKAMKKT
Sbjct: 200 RIVVGKKFSTAVDASNNENEEYRKALRDFFEWFGVFVPSDSFPFLKWLDLGGHEKAMKKT 259

Query: 64  AKVLDSVLDLWMEDHRKKRNSGEVEMHGNDFMDVMLSVFKDDGGQRFGYDVHTITKATCL 123
           AKVLD V D W++DH  K N GEV+M  +DFMDVMLS  +DD GQ   YD HT+TKATCL
Sbjct: 260 AKVLDEVFDKWIQDH--KNNLGEVKMEEHDFMDVMLSNVRDD-GQLSKYDAHTVTKATCL 319

Query: 124 AIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLLYLQAIV 183
           ++IL G DTT+ TM WAL+LLLN++E LK+ Q+ELDE++GR+R+V+ESDVKNLLYLQAIV
Sbjct: 320 SLILAGSDTTTVTMIWALSLLLNNQEVLKRAQLELDERVGRQRQVKESDVKNLLYLQAIV 379

Query: 184 KETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNEFRPERF 243
           KETLRLYPAAPI +PH+++++C VAGYHI   T+LIVN++KLQRDP IW+DP EFRPERF
Sbjct: 380 KETLRLYPAAPILVPHESVDDCVVAGYHIPVGTRLIVNVQKLQRDPQIWEDPCEFRPERF 439

Query: 244 LTTQKHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQELLDME 303
           LT+ K F+V GQ+P LIPFG+GR+MCPGISFAL++MHL LANLLH FE  RPS+E LDME
Sbjct: 440 LTSAKDFDVRGQNPRLIPFGSGRRMCPGISFALQVMHLALANLLHGFEISRPSKEFLDME 499

Query: 304 EGVGISCVKKNPLQIVLTPRLPIRVYD 329
           E  G++ ++KNPL++VLTPRLP  VY+
Sbjct: 500 ESAGMTSIRKNPLEVVLTPRLPPHVYE 523

BLAST of Sed0022534 vs. ExPASy TrEMBL
Match: A0A0A0LG04 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852630 PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.8e-123
Identity = 218/329 (66.26%), Postives = 274/329 (83.28%), Query Frame = 0

Query: 4   RMVVGERFTAGLD---GGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYEKAMKK 63
           R+VVG++F+  +D        EYR A+RDFFE FG FVPSDSFPFLKW D GG+EKAMKK
Sbjct: 210 RIVVGKQFSTAVDVSNTNENEEYRKALRDFFEWFGVFVPSDSFPFLKWLDLGGHEKAMKK 269

Query: 64  TAKVLDSVLDLWMEDHRKKRNS-GEVEMHGNDFMDVMLSVFKDDGGQRFGYDVHTITKAT 123
           TA+VLD V D W+++H++K+N+ G V+M  +DFMDVMLS  +DD GQ   YD H +TKAT
Sbjct: 270 TAQVLDEVFDKWIQEHQQKKNNLGAVKMEEHDFMDVMLSNVRDD-GQLSKYDAHIVTKAT 329

Query: 124 CLAIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLLYLQA 183
           CLA+IL G DTT+ TM WAL+LLLN++E LK+ Q+ELDE +GR+R+V+ESDVKNLLYLQA
Sbjct: 330 CLALILAGSDTTTVTMIWALSLLLNNQEVLKRAQLELDEHVGRQRQVKESDVKNLLYLQA 389

Query: 184 IVKETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNEFRPE 243
           +VKETLRLYPAAPI IPH++IE+C VAGYHI   T+LIVN++KLQRDP IW+DP EF PE
Sbjct: 390 VVKETLRLYPAAPILIPHESIEDCVVAGYHIPLGTRLIVNVQKLQRDPQIWEDPCEFHPE 449

Query: 244 RFLTTQKHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQELLD 303
           RFLT++K F+V GQSP+LIPFG+GR+MCPGISFAL++MHL LANLLH FE  RP++ELLD
Sbjct: 450 RFLTSEKDFDVRGQSPQLIPFGSGRRMCPGISFALQVMHLALANLLHGFEISRPTKELLD 509

Query: 304 MEEGVGISCVKKNPLQIVLTPRLPIRVYD 329
           MEE  G++ ++KNPL++VLTPRLP +VY+
Sbjct: 510 MEESAGMTSIRKNPLEVVLTPRLPPQVYE 537

BLAST of Sed0022534 vs. ExPASy TrEMBL
Match: A0A6J1DBE5 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111018907 PE=3 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 3.8e-121
Identity = 206/325 (63.38%), Postives = 268/325 (82.46%), Query Frame = 0

Query: 4   RMVVGERFTAGLDGGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYEKAMKKTAK 63
           R+VVG+RF+   +G  G +Y  A+ DFF+LFGAF+PSDSFPFL W D GGYEKAM+ TAK
Sbjct: 196 RIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDSFPFLSWLDLGGYEKAMRNTAK 255

Query: 64  VLDSVLDLWMEDHRKKRNSGEVEMHGNDFMDVMLSVFKDDGGQRFGYDVHTITKATCLAI 123
           VLD VLD W+++HR++ +   VEM  +DFMDVMLS  KDD  +  GYDV T+ KATCLA+
Sbjct: 256 VLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDD--ELSGYDVDTVAKATCLAL 315

Query: 124 ILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLLYLQAIVKE 183
           ILGG+ TTS  M WAL+LLLN+EEALKK+Q+ELDEQ+GRER+V ESD+KNL+YLQA+VKE
Sbjct: 316 ILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESDIKNLIYLQAVVKE 375

Query: 184 TLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNEFRPERFLT 243
           TLRLY AAP+++PH++IE+CTVAGYHI   T+L VNL KLQRDPL+W++P EF PERF+T
Sbjct: 376 TLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPLVWENPGEFCPERFIT 435

Query: 244 TQKHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQELLDMEEG 303
           +QK+F+V GQ+P+LIPFG GR++CPG+SFA+++MHLTLA LLH FE  RPS+E L+MEE 
Sbjct: 436 SQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFEIKRPSEEPLNMEES 495

Query: 304 VGISCVKKNPLQIVLTPRLPIRVYD 329
            G++  +K PL++VL+ RL  ++Y+
Sbjct: 496 AGLNITRKTPLEVVLSQRLSAQIYE 518

BLAST of Sed0022534 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 323.6 bits (828), Expect = 1.9e-88
Identity = 160/331 (48.34%), Postives = 230/331 (69.49%), Query Frame = 0

Query: 1   MMCRMVVGERFTAGLDGGA--------GAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFG 60
           M+ RMV G+R+  G  GGA          ++R  +  FF L G F  SD+FP L W D  
Sbjct: 178 MIMRMVAGKRYFGG--GGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDLQ 237

Query: 61  GYEKAMKKTAKVLDSVLDLWMEDHRKKRNSGEVEMHGNDFMDVMLSVFKDDGGQRFGYDV 120
           G+EK MK+T + LD +L+ W+E+HR++R     + + +DF+DVMLS+ +        YD 
Sbjct: 238 GHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKLSHLQYDA 297

Query: 121 HTITKATCLAIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVK 180
           +T  K TCLA+ILGG +T+ +T+ WA++LLLN+++ LKK+Q E+D  +GR+R VE+SD+K
Sbjct: 298 NTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIK 357

Query: 181 NLLYLQAIVKETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKD 240
           NL+YLQAI+KETLRLYPAAP+    +A+E+CTVAGY++   T+LIVN+ K+QRDP ++ +
Sbjct: 358 NLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYME 417

Query: 241 PNEFRPERFLTTQ-KHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFG 300
           PNEFRPERF+T + K F+V GQ+ EL+PFG+GR+ CPG S A++M+HL LA  LH FE  
Sbjct: 418 PNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVK 477

Query: 301 RPSQELLDMEEGVGISCVKKNPLQIVLTPRL 323
                 +DM E  G++  K  PL++++ PRL
Sbjct: 478 TVLDRPVDMSESPGLTITKATPLEVLINPRL 506

BLAST of Sed0022534 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 319.3 bits (817), Expect = 3.6e-87
Identity = 155/329 (47.11%), Postives = 230/329 (69.91%), Query Frame = 0

Query: 1   MMCRMVVGERFTAG------LDGGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGY 60
           M+ RMV G+R+  G       D     + + A+  FF L G F  SD+FP L +FD  G+
Sbjct: 190 MIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGH 249

Query: 61  EKAMKKTAKVLDSVLDLWMEDHRKKRNSGEVEMHGNDFMDVMLSVFKDDGGQRFGYDVHT 120
           EK MK+T   LD +L+ W+E+HR++R     + + +DF+DVM+S+ +        YD +T
Sbjct: 250 EKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHLQYDANT 309

Query: 121 ITKATCLAIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNL 180
             K+TCLA+ILGG DT+++T+ WA++LLLN++E LKK Q E+D  +GR+R VE+SD++NL
Sbjct: 310 SIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENL 369

Query: 181 LYLQAIVKETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPN 240
           +YLQAI+KETLRLYPA P+  P +A+E+CTVAGY++   T+LIVN+ K+QRDP ++ +PN
Sbjct: 370 VYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPN 429

Query: 241 EFRPERFLTTQ-KHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRP 300
           EFRPERF+T + K F+V GQ+ EL+PFG+GR+ CPG S A++++HL LA  LH F+    
Sbjct: 430 EFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTV 489

Query: 301 SQELLDMEEGVGISCVKKNPLQIVLTPRL 323
               +DM E  G++  K  PL+++++PR+
Sbjct: 490 MDMPVDMSENPGLTIPKATPLEVLISPRI 518

BLAST of Sed0022534 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 315.8 bits (808), Expect = 3.9e-86
Identity = 156/333 (46.85%), Postives = 230/333 (69.07%), Query Frame = 0

Query: 1   MMCRMVVGERFTAG-----LDGGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYE 60
           MM RMV G+R+  G      D     + R  V +FF L G F  SD+FP L WFDF G+E
Sbjct: 190 MMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGHE 249

Query: 61  KAMKKTAKVLDSVLDLWMEDHRKKRNSGEVEMHGNDFMDVMLSVFKDDGGQRFGYDVHTI 120
           K MK+T + LD +L+ W+E+HR++R     + + +DF+DVMLS+ +        +D  T 
Sbjct: 250 KEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHLQHDAITS 309

Query: 121 TKATCLAIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLL 180
            K+TCLA+ILGG +T+ +T+ WA++LLLN+++ LKK Q E+D  +GR+R VE+SD++NL+
Sbjct: 310 IKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLV 369

Query: 181 YLQAIVKETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNE 240
           Y+QAI+KETLRLYPA P+    +AIE+CTVAGY++   T+++VN+ K+QRDP ++ +PNE
Sbjct: 370 YIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNE 429

Query: 241 FRPERFLTTQ-KHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPS 300
           FRPERF+T + K F+V GQ+ EL+PFG+GR+ CPG S A++++HL LA  L  F+     
Sbjct: 430 FRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVM 489

Query: 301 QELLDMEEGVGISCVKKNPLQIVLTPRLPIRVY 328
              +DM E  G++  K  PL+I+++PRL   +Y
Sbjct: 490 DMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522

BLAST of Sed0022534 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 244.6 bits (623), Expect = 1.1e-64
Identity = 127/307 (41.37%), Postives = 187/307 (60.91%), Query Frame = 0

Query: 27  VRDFFELFGAFVPSDSFPFLKWFDFGGYEKAMKKTAKVLDSVLDLWMEDHRKKRNSGEVE 86
           +R+F + F  F+ SD  P L + D+   ++ MK+TAK LD V + W+E+H+ KR+     
Sbjct: 220 IREFLDYFALFLLSDVAPVLGFLDW-KTKRGMKRTAKGLDKVAEGWIEEHKNKRSD---- 279

Query: 87  MHG---NDFMDVMLSVFKDDGGQRFGYDVHTITKATCLAIILGGWDTTSATMEWALTLLL 146
            HG   ND++D+++ +   D       D HT  KA CL ++L G +T    + WA++LLL
Sbjct: 280 -HGRSENDYLDILIKILGQDKIPGLS-DTHTKIKALCLNLVLAGSETAIVVLVWAVSLLL 339

Query: 147 NDEEALKKIQIELDEQIGRERKVEESDVKNLLYLQAIVKETLRLYPAAPIAIPHKAIENC 206
           N+   L+K Q ELD +IG+ER VEE D+K+L+YLQAIVKET RLYP  P+      +E+ 
Sbjct: 340 NNPHVLRKAQEELDSKIGKERVVEELDIKDLVYLQAIVKETFRLYPPVPLVAYRAVVEDF 399

Query: 207 TVA--GYHISTKTQLIVNLKKLQRDPLIWKDPNEFRPERFLTTQKHFNVSGQSPELIPFG 266
            +A    H+   TQL+V+  K+ RDP +W +P +F PERFLT+ +  +V GQS +  PFG
Sbjct: 400 DIAFCKCHVPAGTQLMVSAWKIHRDPNVWSNPEQFEPERFLTSNRELDVGGQSYKFFPFG 459

Query: 267 NGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQELLDMEEGVGISCVKKNPLQIVLTPR 326
            GR+ CP I   +RM+H  L   LH F+  RPS + +DM E  G+   K  PL++ + PR
Sbjct: 460 LGRRSCPAIPLGMRMVHYLLVRFLHSFDLARPSSQDVDMTESNGLVNHKATPLEVNIIPR 519

Query: 327 LPIRVYD 329
           L   +Y+
Sbjct: 520 LHKSLYE 519

BLAST of Sed0022534 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 244.2 bits (622), Expect = 1.5e-64
Identity = 138/327 (42.20%), Postives = 196/327 (59.94%), Query Frame = 0

Query: 1   MMCRMVVGERFTAGLDGGAGAEYRNAVRDFFELFGAFVPSDSFPFLKWFDFGGYEKAMKK 60
           ++ R +VG+R   G        Y+ A++    L    +  D  P+L W DF      MK+
Sbjct: 191 IILRKMVGKRIGFGEVNSDEWRYKEALKHCEYLAVIPMIGDVIPWLGWLDF-AKNSQMKR 250

Query: 61  TAKVLDSVLDLWMEDHRKKRNSGEVEMHGNDFMDVMLSVFKDDGGQRFGYDVHTITKATC 120
             K LDSV   W+ +H KKR+  E +      MD++L +  +D     G+    I KAT 
Sbjct: 251 LFKELDSVNTKWLHEHLKKRSRNEKDQE-RTIMDLLLDILPED-IVISGHVRDVIVKATI 310

Query: 121 LAIILGGWDTTSATMEWALTLLLNDEEALKKIQIELDEQIGRERKVEESDVKNLLYLQAI 180
           LA+ L G D+TS T+ WA++LLLN+  AL+  Q E+D  +G+ R +EESD++NL YLQAI
Sbjct: 311 LALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDIQNLKYLQAI 370

Query: 181 VKETLRLYPAAPIAIPHKAIENCTVAGYHISTKTQLIVNLKKLQRDPLIWKDPNEFRPER 240
           VKET RLYP AP+    +A E+C V GY +   T+L+VN+ KL RDP IW DP  F+PER
Sbjct: 371 VKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDPKTFKPER 430

Query: 241 FLTTQKHFNVSGQSPELIPFGNGRKMCPGISFALRMMHLTLANLLHMFEFGRPSQELLDM 300
           F+  +     S  + E IPFG+GR+ CPG++  LR++H  LA LL  FE  + S E LDM
Sbjct: 431 FMEDKSQCEKS--NFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSDEPLDM 490

Query: 301 EEGVGISCVKKNPLQIVLTPRLPIRVY 328
            EG G++  K NP+++V+ PRL  ++Y
Sbjct: 491 AEGPGLALPKINPVEVVVMPRLDPKLY 512

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022935739.13.4e-12468.20cytochrome P450 CYP82D47-like [Cucurbita moschata][more]
XP_008464765.17.6e-12466.97PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo] >KAA0032725.1 cytochrome... [more]
XP_023535456.12.9e-12367.18cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo][more]
XP_004147803.23.8e-12366.26cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59914.1 hypothetical protein Csa_... [more]
XP_038899514.11.9e-12264.72cytochrome P450 CYP82D47-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O493962.7e-8748.34Cytochrome P450 82C3 OS=Arabidopsis thaliana OX=3702 GN=CYP82C3 PE=2 SV=3[more]
A0A0N7F2977.8e-8749.85Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP... [more]
Q9SZ465.0e-8647.11Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=... [more]
O493945.6e-8546.85Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=... [more]
O498584.7e-8448.34Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F6971.7e-12468.20cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442562 PE=3... [more]
A0A5A7SU103.7e-12466.97Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3CM783.7e-12466.97cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502573 PE=3 SV=1[more]
A0A0A0LG041.8e-12366.26Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852630 PE=3 SV=1[more]
A0A6J1DBE53.8e-12163.38cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111018907 PE=... [more]
Match NameE-valueIdentityDescription
AT4G31950.11.9e-8848.34cytochrome P450, family 82, subfamily C, polypeptide 3 [more]
AT4G31940.13.6e-8747.11cytochrome P450, family 82, subfamily C, polypeptide 4 [more]
AT4G31970.13.9e-8646.85cytochrome P450, family 82, subfamily C, polypeptide 2 [more]
AT2G25160.11.1e-6441.37cytochrome P450, family 82, subfamily F, polypeptide 1 [more]
AT3G25180.11.5e-6442.20cytochrome P450, family 82, subfamily G, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 126..143
score: 38.94
coord: 179..190
score: 46.11
coord: 258..267
score: 47.61
coord: 267..278
score: 32.79
IPR001128Cytochrome P450PFAMPF00067p450coord: 2..310
e-value: 2.7E-68
score: 230.8
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 267..290
score: 32.92
coord: 219..243
score: 41.39
coord: 135..161
score: 34.18
coord: 115..132
score: 26.52
coord: 257..267
score: 44.55
coord: 178..196
score: 36.52
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..321
e-value: 8.5E-83
score: 280.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 2..321
NoneNo IPR availablePANTHERPTHR47947:SF26CYTOCHROME P450coord: 1..327
NoneNo IPR availablePANTHERPTHR47947CYTOCHROME P450 82C3-RELATEDcoord: 1..327
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 260..269

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0022534.1Sed0022534.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098542 defense response to other organism
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen