Sed0022452 (gene) Chayote v1

BLAST of Sed0022452 vs. NCBI nr
Match: XP_011659746.1 (acetylajmalan esterase-like [Cucumis sativus])

HSP 1 Score: 602.4 bits (1552), Expect = 2.6e-168
Identity = 288/371 (77.63%), Postives = 316/371 (85.18%), Query Frame = 0

Query: 1   MAASTSFGFSILLLLLILST-FSNAHLLKLCTFDAIYQLGDSISDTGNLIRENPHTPFSR 60
           MA ST    S+ LLLL+L    S AH LK C FDAIYQLGDSISDTGNLIRENP+TPFS 
Sbjct: 1   MAPSTKSSLSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENPNTPFSH 60

Query: 61  LPYGETLFNHATGRCSNGRLMIDYFALDAGLPLVNPYLNKDALKDHGVNFAVAGSTALPF 120
           LPYG++ FN+ TGRCSNG LM+D+ ALDAGLPLV+PYLNKD L DHGVNFAVAGSTALP 
Sbjct: 61  LPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPS 120

Query: 121 EVLLQN-RILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNY 180
           + L  + +I+SPVTNSSLDHQL+WMFSHFNS CH QRECN KLRSALFLVGEIGGNDYNY
Sbjct: 121 QYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNY 180

Query: 181 ALLQGKTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNN 240
           AL QGKT+ + KD +VP+VVQ IK+AV+KVIS+GATR+VVPGNFPIGCFPIYL+ F TN+
Sbjct: 181 ALFQGKTIQEAKD-MVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTND 240

Query: 241 TSAYDEHHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLG 300
           TSAYDE HCLKDLN+FA+YHNDQIKQ IE LKKENPHAVIVYGDYYNAFLWIIRHAFVLG
Sbjct: 241 TSAYDELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAVIVYGDYYNAFLWIIRHAFVLG 300

Query: 301 FDETSLQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLI 360
           FDE SLQKSCCGIGG+Y FNL QMCG   V  CPNP E ISWDG+HLTQ TY  M  WLI
Sbjct: 301 FDEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLI 360

Query: 361 HDIFPKLHCFV 370
           HDIFPKLHC V
Sbjct: 361 HDIFPKLHCIV 370

BLAST of Sed0022452 vs. NCBI nr
Match: XP_022156428.1 (acetylajmalan esterase-like [Momordica charantia])

HSP 1 Score: 599.4 bits (1544), Expect = 2.2e-167
Identity = 282/364 (77.47%), Postives = 313/364 (85.99%), Query Frame = 0

Query: 4   STSFGFSILLLLLILSTFSNAHLLKLCTFDAIYQLGDSISDTGNLIRENPHTPFSRLPYG 63
           ++SF   + LL L+LS+ S AH L+ C FDAIYQLGDSISDTGNLIRENP+T FS LPYG
Sbjct: 7   TSSFSLLLPLLFLLLSSASTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTFSHLPYG 66

Query: 64  ETLFNHATGRCSNGRLMIDYFALDAGLPLVNPYLNKDALKDHGVNFAVAGSTALPFEVLL 123
           ET F+ ATGRCSNG LM+DYFALDAGLPLVNPYLN+DAL +HGVNFAVAGSTAL FE LL
Sbjct: 67  ETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTALSFEALL 126

Query: 124 QNRILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNYALLQG 183
           +N+ILSPVTNSSLDHQL WM SHF S C  +R+C  KLRSALFLVGEIGGNDYNYAL QG
Sbjct: 127 RNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYNYALFQG 186

Query: 184 KTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNNTSAYD 243
           KT+ +VKD +VP+VVQ IK AV+KVIS+GATR++VPGNFPIGC PIYL+ FQTN+T+AYD
Sbjct: 187 KTIQEVKD-MVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAYD 246

Query: 244 EHHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFDETS 303
           E HCLK LN+ ASYHNDQIKQ IE LK+ENPH VIVYGDYYNA LWI+RHAFVLGFDE S
Sbjct: 247 ELHCLKGLNSLASYHNDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLGFDEAS 306

Query: 304 LQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLIHDIFP 363
           LQKSCCGIGGNYNFNL ++CG P VPVCPNP E ISWDGIHLTQ  Y  MA WLIHDIFP
Sbjct: 307 LQKSCCGIGGNYNFNLMKICGLPGVPVCPNPHEHISWDGIHLTQKAYKFMAYWLIHDIFP 366

Query: 364 KLHC 368
           KLHC
Sbjct: 367 KLHC 369

BLAST of Sed0022452 vs. NCBI nr
Match: XP_038877043.1 (LOW QUALITY PROTEIN: GDSL esterase/lipase At5g03980-like, partial [Benincasa hispida])

HSP 1 Score: 598.6 bits (1542), Expect = 3.7e-167
Identity = 283/367 (77.11%), Postives = 317/367 (86.38%), Query Frame = 0

Query: 1   MAASTSFGFSILLLLLILSTFSNAHLLKLCTFDAIYQLGDSISDTGNLIRENPHTPFSRL 60
           MAAST   +  + L L LS+  NAHLLK+C FDAIYQLGDSISDTGNLIREN +TPFS L
Sbjct: 1   MAASTKSLWISIFLFLFLSSSFNAHLLKVCMFDAIYQLGDSISDTGNLIRENLNTPFSHL 60

Query: 61  PYGETLFNHATGRCSNGRLMIDYFALDAGLPLVNPYLNKDALKDHGVNFAVAGSTALPFE 120
           PYGE+ FN+ TGRCSNG LMIDYFALDAGLPLV+PYLNKDAL +HGVNFAVAGSTAL  E
Sbjct: 61  PYGESFFNNPTGRCSNGLLMIDYFALDAGLPLVSPYLNKDALTNHGVNFAVAGSTALSSE 120

Query: 121 VLLQNRILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNYAL 180
           +L Q +I SPVTNSSL HQL+WMFSHFNS C+ QR+CN KL +ALFLVGEIGGNDYNYAL
Sbjct: 121 LLFQKKISSPVTNSSLSHQLDWMFSHFNSICYNQRDCNEKLYNALFLVGEIGGNDYNYAL 180

Query: 181 LQGKTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNNTS 240
            QGKT+ +VKD +VP+VVQ+IK+AV++VIS+GATR+VVPGNFPIGCFPIYL+AFQTN+TS
Sbjct: 181 FQGKTIQEVKD-MVPDVVQSIKSAVERVISYGATRVVVPGNFPIGCFPIYLTAFQTNDTS 240

Query: 241 AYDEHHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFD 300
           AYDE HCLKDLN FASYHNDQ+K+ IE LKKENP  VIVYGDYYNAFLWI+RHAFVLGFD
Sbjct: 241 AYDELHCLKDLNGFASYHNDQVKEAIEVLKKENPXTVIVYGDYYNAFLWILRHAFVLGFD 300

Query: 301 ETSLQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLIHD 360
           E SLQKSCCG+GGNYNFNL +MCG P V VC NP E ISWDG+HLTQ  Y  MA WLIH 
Sbjct: 301 EGSLQKSCCGVGGNYNFNLMKMCGLPGVEVCQNPNEHISWDGVHLTQKAYKFMAHWLIHA 360

Query: 361 IFPKLHC 368
           IFP+LHC
Sbjct: 361 IFPELHC 366

BLAST of Sed0022452 vs. NCBI nr
Match: XP_031737213.1 (acetylajmalan esterase-like [Cucumis sativus])

HSP 1 Score: 595.5 bits (1534), Expect = 3.1e-166
Identity = 284/371 (76.55%), Postives = 314/371 (84.64%), Query Frame = 0

Query: 1   MAASTSFGFSI-LLLLLILSTFSNAHLLKLCTFDAIYQLGDSISDTGNLIRENPHTPFSR 60
           MAAST   FS+ LLLLL++   S AH LK C FDAIYQLGDSISDTGNLIRENP+TPFS 
Sbjct: 1   MAASTKSSFSLFLLLLLVVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENPNTPFSH 60

Query: 61  LPYGETLFNHATGRCSNGRLMIDYFALDAGLPLVNPYLNKDALKDHGVNFAVAGSTALPF 120
           LPYG++ FN+ TGRCSNG LM+D+FALDAGLPLV PYLNKD   DHGVNFAVAGSTALP 
Sbjct: 61  LPYGQSFFNNPTGRCSNGLLMLDFFALDAGLPLVTPYLNKDGWMDHGVNFAVAGSTALPS 120

Query: 121 EVLLQN-RILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNY 180
           + L  N +ILSPVTNSSLDHQL WMFSHFNS CH QR+CN KLRSALFLVGEIGGNDYNY
Sbjct: 121 QYLSTNYKILSPVTNSSLDHQLEWMFSHFNSICHDQRDCNEKLRSALFLVGEIGGNDYNY 180

Query: 181 ALLQGKTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNN 240
           AL QGKT+ + K H+VP+VV+ IK+AV+KVIS+GATR+VVPGNFPIGCFPIYL+ F TN+
Sbjct: 181 ALFQGKTIQEAK-HMVPDVVRTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTND 240

Query: 241 TSAYDEHHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLG 300
           TSAYDE HCLKDLN  A+YHNDQIKQTIE LKKENP  VIVYGDYYNAFLW+IRHAFVLG
Sbjct: 241 TSAYDELHCLKDLNGLATYHNDQIKQTIEVLKKENPQTVIVYGDYYNAFLWVIRHAFVLG 300

Query: 301 FDETSLQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLI 360
           +DE SLQKSCCGIGG+Y FNL +MCG   V  CPNP E ISWDG+HLTQNTY  M  WLI
Sbjct: 301 YDEESLQKSCCGIGGDYKFNLMKMCGAAGVEACPNPNEHISWDGVHLTQNTYKFMTHWLI 360

Query: 361 HDIFPKLHCFV 370
           H IFPKLHC +
Sbjct: 361 HHIFPKLHCSI 370

BLAST of Sed0022452 vs. NCBI nr
Match: XP_016903430.1 (PREDICTED: acetylajmalan esterase-like [Cucumis melo])

HSP 1 Score: 593.6 bits (1529), Expect = 1.2e-165
Identity = 285/371 (76.82%), Postives = 311/371 (83.83%), Query Frame = 0

Query: 1   MAASTSFGFSILLLLLILST-FSNAHLLKLCTFDAIYQLGDSISDTGNLIRENPHTPFSR 60
           M AST    S+ LLL +L    S AH LK   FDAIYQLGDSISDTGNLIRENP+TPFS 
Sbjct: 1   MGASTKSSLSLFLLLFVLVLGSSKAHPLKAFMFDAIYQLGDSISDTGNLIRENPNTPFSH 60

Query: 61  LPYGETLFNHATGRCSNGRLMIDYFALDAGLPLVNPYLNKDALKDHGVNFAVAGSTALPF 120
           LPYG++ FN+ TGRCSNG LM+D+FALDAGLPLV+PYLNKD L DHGVNFAVAGSTAL  
Sbjct: 61  LPYGQSFFNNPTGRCSNGLLMLDFFALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSS 120

Query: 121 EVL-LQNRILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNY 180
           + L  + +ILSPVTNSSLDHQL WMFSHFNS CH QR+CN KLRSALF VGEIGGNDYNY
Sbjct: 121 QYLSTKYKILSPVTNSSLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNY 180

Query: 181 ALLQGKTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNN 240
           AL QGKT+ + KD +VP+VVQ IK+AV+KVIS+GATR+VVPGNFPIGCFPIYL+ F TN+
Sbjct: 181 ALFQGKTIQEAKD-MVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTND 240

Query: 241 TSAYDEHHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLG 300
           TSAYDE HCLKDLN FA+YHNDQIKQTIE LKKENPH VIVYGDYYNAFLWIIRHA VLG
Sbjct: 241 TSAYDELHCLKDLNGFATYHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLG 300

Query: 301 FDETSLQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLI 360
           FDE SLQKSCCGIGG+YNFNL QMCG   V  CPNP E ISWDG+HLTQ TY  M  WLI
Sbjct: 301 FDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLI 360

Query: 361 HDIFPKLHCFV 370
           HDIFPKLHC V
Sbjct: 361 HDIFPKLHCIV 370

BLAST of Sed0022452 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.3e-82
Identity = 161/364 (44.23%), Postives = 220/364 (60.44%), Query Frame = 0

Query: 8   GFSILL-LLLILSTFSNAHLLKLCTFDAIYQLGDSISDTGNLIRENPHTP---FSRLPYG 67
           GF+ LL L+  L  F+      +C FD+IYQLGDS SDTGNLIR  P  P    +  PYG
Sbjct: 2   GFARLLHLVFSLLVFAGITNGLICPFDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYG 61

Query: 68  ETLFNHATGRCSNGRLMIDYFALDAGLPLVNPYLNKDALKDHGVNFAVAGSTALPFEVLL 127
           ET     TGRCS+GRL+ID+ A    LPL+NPYL ++    HGVNFAVAG+TAL    L 
Sbjct: 62  ETFPGTPTGRCSDGRLIIDFIATALNLPLLNPYLQQNVSFRHGVNFAVAGATALDRSFLA 121

Query: 128 QNRILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNYALLQG 187
              +     +S L  QLNW  ++  S C T +EC+ KL++ALF++G IG ND NYA    
Sbjct: 122 ARGVQVSDIHSHLSAQLNWFRTYLGSICSTPKECSNKLKNALFILGNIGNNDVNYA-FPN 181

Query: 188 KTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNNTSAYD 247
           +T+++++ + VP + +A+  A +++I  G +R++VPG FPIGC    L+          D
Sbjct: 182 RTIEEIRAY-VPFITEAVANATREIIRLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDKD 241

Query: 248 EHHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFDETS 307
           +  CL  LNN + Y N   ++ + +L  E P AVI+Y DYYNA+ ++ R+   LG + TS
Sbjct: 242 DLGCLSSLNNLSIYFNSLFQRALASLSIEFPQAVIIYADYYNAWRFLFRNGPALGSNSTS 301

Query: 308 LQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLIHDIFP 367
           L K CCGIGG YN++  + CG   VPVCPNP + I WDG H TQ  Y  +A ++I  I  
Sbjct: 302 LLKCCCGIGGPYNYDPDRECGSRGVPVCPNPTQYIQWDGTHFTQAAYRRVAEYVIPGIIK 361

BLAST of Sed0022452 vs. ExPASy Swiss-Prot
Match: Q9LZB2 (GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.1e-73
Identity = 151/365 (41.37%), Postives = 208/365 (56.99%), Query Frame = 0

Query: 4   STSFGFSILLLLLILSTFSNAHLLKLCTFDAIYQLGDSISDTGNLIRENPHTPFSRLPYG 63
           ST+   S+L+ +L +S     H    C  ++IYQ GDSISDTGNLIR  P          
Sbjct: 2   STTKALSLLVFILFVSL---VHSSDQCPINSIYQFGDSISDTGNLIRNGP---------- 61

Query: 64  ETLFNHATGRCSNGRLMIDYFALDAGLPLVNPYLNKDALKDHGVNFAVAGSTALPFEVLL 123
                                   A  P   P   ++   +  VNF V+GSTAL      
Sbjct: 62  ------------------------ASSPTPKPLPQRE--HNVFVNFGVSGSTALNSSFFS 121

Query: 124 QNRILSPVTNSSLDHQLNWMFSHFNSTCH-TQRECNYKLRSALFLVGEIGGNDYNYALLQ 183
           +  +  P TN+ L  QL W   H  STCH +  +C   L+ +LF+VGEIGGNDYNY   Q
Sbjct: 122 ERNLHVPATNTPLSMQLAWFKGHLRSTCHGSSSDC---LKHSLFMVGEIGGNDYNYGFFQ 181

Query: 184 GKTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNNTSAY 243
           GK +++++ + +P+VV AI  A ++VI  GA  +VVPGNFP+GCFPIYL++F   +T  Y
Sbjct: 182 GKPMEEIRSY-IPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKDY 241

Query: 244 DEHHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFDET 303
           D++ CL  LN FA  HN+Q+++ I +L+KE P   IVYGDYYNAF +++R      FD++
Sbjct: 242 DDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSE---RFDKS 301

Query: 304 SLQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLIHDIF 363
              KSCCG GG YN++  +  G   VPVC NP + ISWDG+HLTQ  Y  M+ +L + I 
Sbjct: 302 VALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQIL 320

Query: 364 PKLHC 368
            ++ C
Sbjct: 362 SQIKC 320

BLAST of Sed0022452 vs. ExPASy Swiss-Prot
Match: Q9FXJ2 (GDSL esterase/lipase At1g28580 OS=Arabidopsis thaliana OX=3702 GN=At1g28580 PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 7.9e-72
Identity = 148/358 (41.34%), Postives = 209/358 (58.38%), Query Frame = 0

Query: 12  LLLLLILSTFSNAHL---LKLCTFDAIYQLGDSISDTGNLI-----RENPHTPFSRLPYG 71
           LL+ + LSTF   ++    K   F +I   GDSI+DTGNL+     ++ PH  F   PYG
Sbjct: 12  LLVFIFLSTFVVTNVSSETKCREFKSIISFGDSIADTGNLLGLSDPKDLPHMAFP--PYG 71

Query: 72  ETLFNHATGRCSNGRLMIDYFALDAGLPLVNP-YLNKDALKDHGVNFAVAGSTALPFEVL 131
           E  F+H TGR SNGRL+ID+ A   GLPLV P Y + +A  + GVNFAV G+TAL    L
Sbjct: 72  ENFFHHPTGRFSNGRLIIDFIAEFLGLPLVPPFYGSHNANFEKGVNFAVGGATALERSFL 131

Query: 132 LQNRILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNYALLQ 191
               I  P TN SL  QLN       S C +  +C   + +AL L+GEIGGNDYNYA   
Sbjct: 132 EDRGIHFPYTNVSLGVQLNSFKESLPSICGSPSDCRDMIENALILMGEIGGNDYNYAFFV 191

Query: 192 GKTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNNTSAY 251
            K ++++K+ L+P V+  I +A+ ++I  G    +VPG FP+GC  +YL++ QT+N   Y
Sbjct: 192 DKGIEEIKE-LMPLVITTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTSHQTSNMEEY 251

Query: 252 DE-HHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFDE 311
           D    CLK LN F   H +Q++  +  L+K  PH  I+Y DYYNA   + +     GF  
Sbjct: 252 DPLTGCLKWLNKFGENHGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQEPAKFGFMN 311

Query: 312 TSLQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLIH 360
             L  +CCG GG YN+ + + CG   V  C +P + ++WDG+H+T+  Y +MA  +++
Sbjct: 312 RPL-SACCGAGGPYNYTVGRKCGTDIVESCDDPSKYVAWDGVHMTEAAYRLMAEGILN 365

BLAST of Sed0022452 vs. ExPASy Swiss-Prot
Match: Q9FXJ1 (GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 6.7e-71
Identity = 147/356 (41.29%), Postives = 211/356 (59.27%), Query Frame = 0

Query: 11  ILLLLLILSTF--SNAHLLKLC-TFDAIYQLGDSISDTGNLIRENPHTPFSR---LPYGE 70
           ++   LILSTF  +  +    C  F +I   GDSI+DTGNL+  +  T   +   LPYGE
Sbjct: 8   LVSFFLILSTFCLTTVNSEPQCHNFKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGE 67

Query: 71  TLFNHATGRCSNGRLMIDYFALDAGLPLVNP-YLNKDALKDHGVNFAVAGSTALPFEVLL 130
           T F+H TGR SNGRL+ID+ A   G PLV P Y +++A  + GVNFAV G+TAL    L 
Sbjct: 68  TFFHHPTGRFSNGRLIIDFIAEFLGFPLVPPFYGSQNANFEKGVNFAVGGATALERSFLE 127

Query: 131 QNRILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNYALLQG 190
           +  I  P TN SL  QL+       + C +  +C   + ++L L+GEIGGNDYNYA   G
Sbjct: 128 ERGIHFPYTNVSLAVQLSSFKESLPNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVG 187

Query: 191 KTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNNTSAYD 250
           K ++++K+ LVP V++ I +A+ ++I  G    +VPG FP+GC   YLS +QT+N   YD
Sbjct: 188 KNIEEIKE-LVPLVIETISSAITELIGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIEEYD 247

Query: 251 E-HHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFDET 310
               CLK LN F+ YH++Q++  +  L+K  PH  I+Y DYYN  L + +     GF   
Sbjct: 248 PLTGCLKWLNKFSEYHDEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISR 307

Query: 311 SLQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLI 359
            L  +CC +GG +NF L +  G      C +P + +SWDG+H+T+  Y +MA  ++
Sbjct: 308 PL-PACCALGGPFNFTLGRKRGTQVPECCDDPSKYVSWDGVHMTEAAYRLMAEGIL 361

BLAST of Sed0022452 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.1e-70
Identity = 141/335 (42.09%), Postives = 199/335 (59.40%), Query Frame = 0

Query: 32  FDAIYQLGDSISDTGNLI-----RENPHTPFSRLPYGETLFNHATGRCSNGRLMIDYFAL 91
           F +I   GDSI+DTGNL+      + P T F   PYGET F+H TGR  +GR+++D+ A 
Sbjct: 29  FKSIISFGDSIADTGNLVGLSDRNQLPVTAFP--PYGETFFHHPTGRSCDGRIIMDFIAE 88

Query: 92  DAGLPLVNPYL-NKDALKDHGVNFAVAGSTALPFEVLLQNRILSPVTNSSLDHQLNWMFS 151
             GLP V PY  +K+   D GVNFAVAG+TAL     L+ R + P TN SL  QL     
Sbjct: 89  FVGLPYVPPYFGSKNRNFDKGVNFAVAGATALK-SSFLKKRGIQPHTNVSLGVQLKSFKK 148

Query: 152 HFNSTCHTQRECNYKLRSALFLVGEIGGNDYNYALLQGKTVDQVKDHLVPNVVQAIKTAV 211
              + C +  +C   + +AL L+GEIGGNDYN+     K V +V++ LVP V+ +I + +
Sbjct: 149 SLPNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEE-LVPFVIASISSTI 208

Query: 212 QKVISFGATRIVVPGNFPIGCFPIYLSAFQTNNTSAYD-EHHCLKDLNNFASYHNDQIKQ 271
            ++I  G    +VPG FPIGC  +YL+ ++T+N   YD    CLK LN F  YH++++K 
Sbjct: 209 TELIGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKV 268

Query: 272 TIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFDETSLQKSCCGIGGNYNFNLTQMCG 331
            +  L+K  PH  I+Y DYYN+ L I +     GF E     +CCGIGG YNFN T+ CG
Sbjct: 269 ELNRLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERPF-PACCGIGGPYNFNFTRKCG 328

Query: 332 FPEVPVCPNPEERISWDGIHLTQNTYNIMASWLIH 360
              V  C +P + + WDG+H+T+  Y  +A  +++
Sbjct: 329 SVGVKSCKDPSKYVGWDGVHMTEAAYKWIADGILN 358

BLAST of Sed0022452 vs. ExPASy TrEMBL
Match: A0A6J1DQL3 (acetylajmalan esterase-like OS=Momordica charantia OX=3673 GN=LOC111023320 PE=3 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 1.0e-167
Identity = 282/364 (77.47%), Postives = 313/364 (85.99%), Query Frame = 0

Query: 4   STSFGFSILLLLLILSTFSNAHLLKLCTFDAIYQLGDSISDTGNLIRENPHTPFSRLPYG 63
           ++SF   + LL L+LS+ S AH L+ C FDAIYQLGDSISDTGNLIRENP+T FS LPYG
Sbjct: 7   TSSFSLLLPLLFLLLSSASTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTFSHLPYG 66

Query: 64  ETLFNHATGRCSNGRLMIDYFALDAGLPLVNPYLNKDALKDHGVNFAVAGSTALPFEVLL 123
           ET F+ ATGRCSNG LM+DYFALDAGLPLVNPYLN+DAL +HGVNFAVAGSTAL FE LL
Sbjct: 67  ETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTALSFEALL 126

Query: 124 QNRILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNYALLQG 183
           +N+ILSPVTNSSLDHQL WM SHF S C  +R+C  KLRSALFLVGEIGGNDYNYAL QG
Sbjct: 127 RNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYNYALFQG 186

Query: 184 KTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNNTSAYD 243
           KT+ +VKD +VP+VVQ IK AV+KVIS+GATR++VPGNFPIGC PIYL+ FQTN+T+AYD
Sbjct: 187 KTIQEVKD-MVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAYD 246

Query: 244 EHHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFDETS 303
           E HCLK LN+ ASYHNDQIKQ IE LK+ENPH VIVYGDYYNA LWI+RHAFVLGFDE S
Sbjct: 247 ELHCLKGLNSLASYHNDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLGFDEAS 306

Query: 304 LQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLIHDIFP 363
           LQKSCCGIGGNYNFNL ++CG P VPVCPNP E ISWDGIHLTQ  Y  MA WLIHDIFP
Sbjct: 307 LQKSCCGIGGNYNFNLMKICGLPGVPVCPNPHEHISWDGIHLTQKAYKFMAYWLIHDIFP 366

Query: 364 KLHC 368
           KLHC
Sbjct: 367 KLHC 369

BLAST of Sed0022452 vs. ExPASy TrEMBL
Match: A0A6J1DQD3 (GDSL esterase/lipase At5g03980-like OS=Momordica charantia OX=3673 GN=LOC111023260 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.7e-166
Identity = 280/366 (76.50%), Postives = 310/366 (84.70%), Query Frame = 0

Query: 4   STSFGFSILLLLLILSTFSNAHLLKLCTFDAIYQLGDSISDTGNLIRENPHTPFSRLPYG 63
           S+S    + LL L+LS  S AHLL+ C FDAIYQLGDSISDTGNLIRENP+T FSRLPYG
Sbjct: 7   SSSLSLLLPLLFLLLSPXSTAHLLRTCMFDAIYQLGDSISDTGNLIRENPNTTFSRLPYG 66

Query: 64  ETLFNHATGRCSNGRLMIDYFALDAGLPLVNPYLNKDALKDHGVNFAVAGSTALPFEVLL 123
           ET F+ ATGRCSNG LM+DYFALD GLPLVNPYLNKDALK+ GVNFAVAGSTAL  E L 
Sbjct: 67  ETFFHGATGRCSNGLLMLDYFALDXGLPLVNPYLNKDALKNRGVNFAVAGSTALSSETLF 126

Query: 124 QNRILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNYALLQG 183
           +N+I SPVTNSSLD QL WM SHF S C  +R+C  KLRSALFLVGEIGGNDYNYAL QG
Sbjct: 127 RNKIFSPVTNSSLDRQLXWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYNYALFQG 186

Query: 184 KTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNNTSAYD 243
           KT+ +VKD +VP+VVQ IK AV+KVIS+GATR++VPGNFPIGC PIYL+ FQTN+T+AYD
Sbjct: 187 KTIQEVKD-MVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAYD 246

Query: 244 EHHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFDETS 303
           E HCLK LN+ ASYHNDQIKQ IE LK+ENPH VIVYGDYYNA LWI+RHAFVLGFDE S
Sbjct: 247 ELHCLKGLNSLASYHNDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLGFDEAS 306

Query: 304 LQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLIHDIFP 363
           LQKSCCGIGGNYNFNL ++CG P+VP+CPNP E ISWDGIHLTQ  Y  MA WLIHDIFP
Sbjct: 307 LQKSCCGIGGNYNFNLIKICGLPDVPICPNPHEHISWDGIHLTQKAYKFMAYWLIHDIFP 366

Query: 364 KLHCFV 370
           KLHC V
Sbjct: 367 KLHCIV 371

BLAST of Sed0022452 vs. ExPASy TrEMBL
Match: A0A1S4E629 (acetylajmalan esterase-like OS=Cucumis melo OX=3656 GN=LOC103503259 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.7e-166
Identity = 285/371 (76.82%), Postives = 311/371 (83.83%), Query Frame = 0

Query: 1   MAASTSFGFSILLLLLILST-FSNAHLLKLCTFDAIYQLGDSISDTGNLIRENPHTPFSR 60
           M AST    S+ LLL +L    S AH LK   FDAIYQLGDSISDTGNLIRENP+TPFS 
Sbjct: 1   MGASTKSSLSLFLLLFVLVLGSSKAHPLKAFMFDAIYQLGDSISDTGNLIRENPNTPFSH 60

Query: 61  LPYGETLFNHATGRCSNGRLMIDYFALDAGLPLVNPYLNKDALKDHGVNFAVAGSTALPF 120
           LPYG++ FN+ TGRCSNG LM+D+FALDAGLPLV+PYLNKD L DHGVNFAVAGSTAL  
Sbjct: 61  LPYGQSFFNNPTGRCSNGLLMLDFFALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSS 120

Query: 121 EVL-LQNRILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNY 180
           + L  + +ILSPVTNSSLDHQL WMFSHFNS CH QR+CN KLRSALF VGEIGGNDYNY
Sbjct: 121 QYLSTKYKILSPVTNSSLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNY 180

Query: 181 ALLQGKTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNN 240
           AL QGKT+ + KD +VP+VVQ IK+AV+KVIS+GATR+VVPGNFPIGCFPIYL+ F TN+
Sbjct: 181 ALFQGKTIQEAKD-MVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTND 240

Query: 241 TSAYDEHHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLG 300
           TSAYDE HCLKDLN FA+YHNDQIKQTIE LKKENPH VIVYGDYYNAFLWIIRHA VLG
Sbjct: 241 TSAYDELHCLKDLNGFATYHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLG 300

Query: 301 FDETSLQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLI 360
           FDE SLQKSCCGIGG+YNFNL QMCG   V  CPNP E ISWDG+HLTQ TY  M  WLI
Sbjct: 301 FDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLI 360

Query: 361 HDIFPKLHCFV 370
           HDIFPKLHC V
Sbjct: 361 HDIFPKLHCIV 370

BLAST of Sed0022452 vs. ExPASy TrEMBL
Match: A0A5D3CNR6 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold745G00130 PE=3 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 2.2e-165
Identity = 284/371 (76.55%), Postives = 311/371 (83.83%), Query Frame = 0

Query: 1   MAASTSFGFSILLLLLILST-FSNAHLLKLCTFDAIYQLGDSISDTGNLIRENPHTPFSR 60
           M AST    S+ LLL +L    S AH LK   FDAIYQLGDSISDTGNLIRENP+TPFS 
Sbjct: 1   MGASTKSSLSLFLLLFVLVLGSSKAHPLKAFMFDAIYQLGDSISDTGNLIRENPNTPFSH 60

Query: 61  LPYGETLFNHATGRCSNGRLMIDYFALDAGLPLVNPYLNKDALKDHGVNFAVAGSTALPF 120
           LPYG++ FN+ TGRCSNG LM+D+FALDAGLPLV+PYLNKD L DHGVNFAVAGSTAL  
Sbjct: 61  LPYGQSFFNNPTGRCSNGLLMLDFFALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSS 120

Query: 121 EVL-LQNRILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNY 180
           + L  + +ILSPVTNSSLDHQL WMFSHFNS CH QR+CN KLRSALF VGEIGGNDYNY
Sbjct: 121 QYLSTKYKILSPVTNSSLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNY 180

Query: 181 ALLQGKTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNN 240
           AL QGKT+ + KD +VP+VVQ IK+AV+KVIS+GATR+VVPGNFPIGCFPIYL+ F TN+
Sbjct: 181 ALFQGKTIQEAKD-MVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTND 240

Query: 241 TSAYDEHHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLG 300
           TSAYDE HCLKDLN FA++HNDQIKQTIE LKKENPH VIVYGDYYNAFLWIIRHA VLG
Sbjct: 241 TSAYDELHCLKDLNGFATHHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLG 300

Query: 301 FDETSLQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLI 360
           FDE SLQKSCCGIGG+YNFNL QMCG   V  CPNP E ISWDG+HLTQ TY  M  WLI
Sbjct: 301 FDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLI 360

Query: 361 HDIFPKLHCFV 370
           HDIFPKLHC V
Sbjct: 361 HDIFPKLHCIV 370

BLAST of Sed0022452 vs. ExPASy TrEMBL
Match: A0A5A7SSW2 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold219G001390 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 4.9e-165
Identity = 284/371 (76.55%), Postives = 310/371 (83.56%), Query Frame = 0

Query: 1   MAASTSFGFSILLLLLILST-FSNAHLLKLCTFDAIYQLGDSISDTGNLIRENPHTPFSR 60
           M AST    S+ LLL +L    S AH LK   FDAIYQLGDSISDTGNLIRENP+TPFS 
Sbjct: 1   MGASTKSSLSLFLLLFVLVLGSSKAHPLKAFMFDAIYQLGDSISDTGNLIRENPNTPFSH 60

Query: 61  LPYGETLFNHATGRCSNGRLMIDYFALDAGLPLVNPYLNKDALKDHGVNFAVAGSTALPF 120
           LPYG++ FN+ TGRCSNG LM+D+FALDAGLPLV+PYLNKD L DHGVNFAVAGSTAL  
Sbjct: 61  LPYGQSFFNNPTGRCSNGLLMLDFFALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSS 120

Query: 121 EVL-LQNRILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNY 180
           + L  + +ILSPVTNSSLDHQL WMFSHFNS CH QR+CN KLRSALF VGEIGGNDYNY
Sbjct: 121 QYLSTKYKILSPVTNSSLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNY 180

Query: 181 ALLQGKTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNN 240
           AL QGKT+ + KD +VP+VVQ IK+AV+KVIS+GATR+VVPGNFPIGCFPIYL+ F TN+
Sbjct: 181 ALFQGKTIQEAKD-MVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTND 240

Query: 241 TSAYDEHHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLG 300
           TSAYDE HCLKDLN FA+YHNDQIKQTIE LKKENPH VIVYGDYYNAFLWIIRHA VLG
Sbjct: 241 TSAYDELHCLKDLNGFATYHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLG 300

Query: 301 FDETSLQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLI 360
           FDE SLQKSCCGIGG+YNFNL QMCG   V  CPNP E ISWD +HLTQ TY  M  WLI
Sbjct: 301 FDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEACPNPNEHISWDRVHLTQKTYKFMTHWLI 360

Query: 361 HDIFPKLHCFV 370
           HDIFPKLHC V
Sbjct: 361 HDIFPKLHCIV 370

BLAST of Sed0022452 vs. TAIR 10
Match: AT5G03980.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 278.5 bits (711), Expect = 7.9e-75
Identity = 151/365 (41.37%), Postives = 208/365 (56.99%), Query Frame = 0

Query: 4   STSFGFSILLLLLILSTFSNAHLLKLCTFDAIYQLGDSISDTGNLIRENPHTPFSRLPYG 63
           ST+   S+L+ +L +S     H    C  ++IYQ GDSISDTGNLIR  P          
Sbjct: 2   STTKALSLLVFILFVSL---VHSSDQCPINSIYQFGDSISDTGNLIRNGP---------- 61

Query: 64  ETLFNHATGRCSNGRLMIDYFALDAGLPLVNPYLNKDALKDHGVNFAVAGSTALPFEVLL 123
                                   A  P   P   ++   +  VNF V+GSTAL      
Sbjct: 62  ------------------------ASSPTPKPLPQRE--HNVFVNFGVSGSTALNSSFFS 121

Query: 124 QNRILSPVTNSSLDHQLNWMFSHFNSTCH-TQRECNYKLRSALFLVGEIGGNDYNYALLQ 183
           +  +  P TN+ L  QL W   H  STCH +  +C   L+ +LF+VGEIGGNDYNY   Q
Sbjct: 122 ERNLHVPATNTPLSMQLAWFKGHLRSTCHGSSSDC---LKHSLFMVGEIGGNDYNYGFFQ 181

Query: 184 GKTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNNTSAY 243
           GK +++++ + +P+VV AI  A ++VI  GA  +VVPGNFP+GCFPIYL++F   +T  Y
Sbjct: 182 GKPMEEIRSY-IPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKDY 241

Query: 244 DEHHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFDET 303
           D++ CL  LN FA  HN+Q+++ I +L+KE P   IVYGDYYNAF +++R      FD++
Sbjct: 242 DDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSE---RFDKS 301

Query: 304 SLQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLIHDIF 363
              KSCCG GG YN++  +  G   VPVC NP + ISWDG+HLTQ  Y  M+ +L + I 
Sbjct: 302 VALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQIL 320

Query: 364 PKLHC 368
            ++ C
Sbjct: 362 SQIKC 320

BLAST of Sed0022452 vs. TAIR 10
Match: AT1G28580.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 272.3 bits (695), Expect = 5.6e-73
Identity = 148/358 (41.34%), Postives = 209/358 (58.38%), Query Frame = 0

Query: 12  LLLLLILSTFSNAHL---LKLCTFDAIYQLGDSISDTGNLI-----RENPHTPFSRLPYG 71
           LL+ + LSTF   ++    K   F +I   GDSI+DTGNL+     ++ PH  F   PYG
Sbjct: 12  LLVFIFLSTFVVTNVSSETKCREFKSIISFGDSIADTGNLLGLSDPKDLPHMAFP--PYG 71

Query: 72  ETLFNHATGRCSNGRLMIDYFALDAGLPLVNP-YLNKDALKDHGVNFAVAGSTALPFEVL 131
           E  F+H TGR SNGRL+ID+ A   GLPLV P Y + +A  + GVNFAV G+TAL    L
Sbjct: 72  ENFFHHPTGRFSNGRLIIDFIAEFLGLPLVPPFYGSHNANFEKGVNFAVGGATALERSFL 131

Query: 132 LQNRILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNYALLQ 191
               I  P TN SL  QLN       S C +  +C   + +AL L+GEIGGNDYNYA   
Sbjct: 132 EDRGIHFPYTNVSLGVQLNSFKESLPSICGSPSDCRDMIENALILMGEIGGNDYNYAFFV 191

Query: 192 GKTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNNTSAY 251
            K ++++K+ L+P V+  I +A+ ++I  G    +VPG FP+GC  +YL++ QT+N   Y
Sbjct: 192 DKGIEEIKE-LMPLVITTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTSHQTSNMEEY 251

Query: 252 DE-HHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFDE 311
           D    CLK LN F   H +Q++  +  L+K  PH  I+Y DYYNA   + +     GF  
Sbjct: 252 DPLTGCLKWLNKFGENHGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQEPAKFGFMN 311

Query: 312 TSLQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLIH 360
             L  +CCG GG YN+ + + CG   V  C +P + ++WDG+H+T+  Y +MA  +++
Sbjct: 312 RPL-SACCGAGGPYNYTVGRKCGTDIVESCDDPSKYVAWDGVHMTEAAYRLMAEGILN 365

BLAST of Sed0022452 vs. TAIR 10
Match: AT1G28570.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 4.8e-72
Identity = 147/356 (41.29%), Postives = 211/356 (59.27%), Query Frame = 0

Query: 11  ILLLLLILSTF--SNAHLLKLC-TFDAIYQLGDSISDTGNLIRENPHTPFSR---LPYGE 70
           ++   LILSTF  +  +    C  F +I   GDSI+DTGNL+  +  T   +   LPYGE
Sbjct: 8   LVSFFLILSTFCLTTVNSEPQCHNFKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGE 67

Query: 71  TLFNHATGRCSNGRLMIDYFALDAGLPLVNP-YLNKDALKDHGVNFAVAGSTALPFEVLL 130
           T F+H TGR SNGRL+ID+ A   G PLV P Y +++A  + GVNFAV G+TAL    L 
Sbjct: 68  TFFHHPTGRFSNGRLIIDFIAEFLGFPLVPPFYGSQNANFEKGVNFAVGGATALERSFLE 127

Query: 131 QNRILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNYALLQG 190
           +  I  P TN SL  QL+       + C +  +C   + ++L L+GEIGGNDYNYA   G
Sbjct: 128 ERGIHFPYTNVSLAVQLSSFKESLPNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVG 187

Query: 191 KTVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNNTSAYD 250
           K ++++K+ LVP V++ I +A+ ++I  G    +VPG FP+GC   YLS +QT+N   YD
Sbjct: 188 KNIEEIKE-LVPLVIETISSAITELIGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIEEYD 247

Query: 251 E-HHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFDET 310
               CLK LN F+ YH++Q++  +  L+K  PH  I+Y DYYN  L + +     GF   
Sbjct: 248 PLTGCLKWLNKFSEYHDEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISR 307

Query: 311 SLQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLI 359
            L  +CC +GG +NF L +  G      C +P + +SWDG+H+T+  Y +MA  ++
Sbjct: 308 PL-PACCALGGPFNFTLGRKRGTQVPECCDDPSKYVSWDGVHMTEAAYRLMAEGIL 361

BLAST of Sed0022452 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 268.5 bits (685), Expect = 8.1e-72
Identity = 141/335 (42.09%), Postives = 199/335 (59.40%), Query Frame = 0

Query: 32  FDAIYQLGDSISDTGNLI-----RENPHTPFSRLPYGETLFNHATGRCSNGRLMIDYFAL 91
           F +I   GDSI+DTGNL+      + P T F   PYGET F+H TGR  +GR+++D+ A 
Sbjct: 29  FKSIISFGDSIADTGNLVGLSDRNQLPVTAFP--PYGETFFHHPTGRSCDGRIIMDFIAE 88

Query: 92  DAGLPLVNPYL-NKDALKDHGVNFAVAGSTALPFEVLLQNRILSPVTNSSLDHQLNWMFS 151
             GLP V PY  +K+   D GVNFAVAG+TAL     L+ R + P TN SL  QL     
Sbjct: 89  FVGLPYVPPYFGSKNRNFDKGVNFAVAGATALK-SSFLKKRGIQPHTNVSLGVQLKSFKK 148

Query: 152 HFNSTCHTQRECNYKLRSALFLVGEIGGNDYNYALLQGKTVDQVKDHLVPNVVQAIKTAV 211
              + C +  +C   + +AL L+GEIGGNDYN+     K V +V++ LVP V+ +I + +
Sbjct: 149 SLPNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEE-LVPFVIASISSTI 208

Query: 212 QKVISFGATRIVVPGNFPIGCFPIYLSAFQTNNTSAYD-EHHCLKDLNNFASYHNDQIKQ 271
            ++I  G    +VPG FPIGC  +YL+ ++T+N   YD    CLK LN F  YH++++K 
Sbjct: 209 TELIGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKV 268

Query: 272 TIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFDETSLQKSCCGIGGNYNFNLTQMCG 331
            +  L+K  PH  I+Y DYYN+ L I +     GF E     +CCGIGG YNFN T+ CG
Sbjct: 269 ELNRLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERPF-PACCGIGGPYNFNFTRKCG 328

Query: 332 FPEVPVCPNPEERISWDGIHLTQNTYNIMASWLIH 360
              V  C +P + + WDG+H+T+  Y  +A  +++
Sbjct: 329 SVGVKSCKDPSKYVGWDGVHMTEAAYKWIADGILN 358

BLAST of Sed0022452 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 1.8e-71
Identity = 148/355 (41.69%), Postives = 213/355 (60.00%), Query Frame = 0

Query: 11  ILLLLLILSTFSNAHLLKLCTFDAIYQLGDSISDTGNLI-----RENPHTPFSRLPYGET 70
           IL  LL+ S  S     +   F +I   GDSI+DTGNL+      + P + F   PYGET
Sbjct: 16  ILSTLLVTSVNSQT---QCRNFKSIISFGDSIADTGNLLGLSDPNDLPASAFP--PYGET 75

Query: 71  LFNHATGRCSNGRLMIDYFALDAGLPLVNPYLN-KDALKDHGVNFAVAGSTALPFEVLLQ 130
            F+H TGR S+GRL+ID+ A   G PLV P+   ++A    GVNFAVAG+TAL    L +
Sbjct: 76  FFHHPTGRYSDGRLIIDFIAEFLGFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEE 135

Query: 131 NRILSPVTNSSLDHQLNWMFSHFNSTCHTQRECNYKLRSALFLVGEIGGNDYNYALLQGK 190
             I S +TN SL  QL        + C +  +C   + +AL L+GEIGGNDYN+AL Q K
Sbjct: 136 RGIHSTITNVSLSVQLRSFTESLPNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRK 195

Query: 191 TVDQVKDHLVPNVVQAIKTAVQKVISFGATRIVVPGNFPIGCFPIYLSAFQTNNTSAYDE 250
            V +V++ LVP V+  I +A+ +++  G    +VPGNFPIG    YL+ ++T+N   YD 
Sbjct: 196 PVKEVEE-LVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDP 255

Query: 251 -HHCLKDLNNFASYHNDQIKQTIETLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFDETS 310
              CLK LN+F+ Y+N Q+++ +  L+K  PH  I+Y DYYNA L + +     GF    
Sbjct: 256 LTGCLKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRP 315

Query: 311 LQKSCCGIGGNYNFNLTQMCGFPEVPVCPNPEERISWDGIHLTQNTYNIMASWLI 359
           L  +CCG+GG+YNFN ++ CG   V  C +P + +++DGIH+T+  Y +++  L+
Sbjct: 316 L-PACCGVGGSYNFNFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363