Sed0022128 (gene) Chayote v1

Overview


Name	Sed0022128
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Pectinesterase
Location	LG04: 26862333 .. 26864103 (+)
RNA-Seq Expression	Sed0022128
Synteny	Sed0022128

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATATTACTTTGTAACGAGATTGGCAATCTTCCTTGTAATCTTAGCCAGCAATTTGGCATCATCGTCGCCGGTTCCAGTCGATAAGTCGCAACTAAACAAATGGTTTTCCGACAGTATCAAGCCGCTGGACGCTCGCACAGAGCTTGATAAAGCCGTGGTCGAGGCCGAGAAAGACGCGACCATGGTGAAAGTAAGGGCAGACGGGAGTGGAAATTTCAAGACGATAACGGAAGCCATAAACAGCGTTCCAGCCAACAACAAAAAACGCGTACTCATTTGGATTGGAGGAGGAGTTTACAAAGAGAAGCTTAAGATTGAAATAAATAAACCATTCATTACTTTTTATGGATCTCCTAACGATATGCCAACTTTGACTTTTGATGGTGATTCCAAAACTTTCGGAACCGTTGACAGCGGCACTTTGACTGTCGAATCTGATTATTTTGTTGCTGCCAATATCATCCTTAAGGTAAATTGCATGCATGCAATGTTTAAAATTTTTAATCTACTCTATAGTTCCAAAAAAGTTCAAATTCAATTTTGATGATTTGAGTTTAATCTTTTGGAAAAAGTTAAAGAATTTAATTTTATAGTTTTAAAAATTAGAATTTAAATATATATATTTTTGTTATATCAATCATTTAATTTATTCTTTCAAAAAAAAGAAAAATCACTTAATTTATAATTAGAAAATTGTGCCTGGATAGTTCAATTTCACAAAATAATTAGAGATTGAAGAAAATTTGATTGCATTCATATTAATTATATCGTATATTTGACTATGGTTGTTAAATCTTTAGTTTTGGCTAGTCTTATAAATATTTTTCCAATTTGACCTATCCCTATATAATTATTGGGCAATTTAAGTTTTATCGAATTATAGAGACTAATTTTTTTAAACCATAGGAAGTAAACTTAAAATATTTTGTGGAATGATTATATTATCAGAATTCATCCCCAAGGCCAAACAAAGAGGGAGGAGGACCGGCATTAGCGGCAAGAATAATGGGAAACAAAGCCGCAATTTACAATTGCAAATTCTATGGGTTTCAAGACACCCTTTGCGACGACGCCGGCATGCATCTTTACAAAGATTGCTTCATTCAAGGCACCACAGACTTCATTTTTGGGAGAGGAACCTCCCTTTATTTGGTATTTTTTTTATCCAATTTATCATTTAACTAATTATAAATTTCATAGTTTAGAAGTGGTGCGTATAGATTTTTGTTGTTTATTTAGAATGTGTATTTTGTGAATATAATCGCATGCAGAACTGTCAATTGGATGTGGTGAAAGAGGGTTCGGTGATCGCAGCACATTCGAGAGAGCTTGAACATGACGGAAGCGGCTACTCGTTTGTGCATTGCACCATTACCGGACCGGATACGAAGAGAATTTATTTGGGAAGAGCTTGGAGGCCTAGGTCTAGAGTTGTATACTCCTACACCAAAATGGCAGACAATGTTCACCCTGAAGGCTGGCACGACATGGGAAATCCCAAATACACTTCGTAATTATAAAATCCTTCTCTTCTTTTTAGTTATATAGTATGAGTGTGAACTCTTTTCTTTTTTTCTAATGATGATTCATAATGTTGCAGCTCGATTTTGTTCGGAGAATACAAGTGTTCAGGGCCGGGATCACAAACGGATCAGCGCGTATCCTACAGTAAACGATTGAGTGACGCAGAAGCGAAGCCATACCTTAGCCTTAATTACATAGAGGCTGCCAAATGGTTGCTTCCTCCTCCCAGCCTAAAGGCTTAA

mRNA sequence

ATGAAATATTACTTTGTAACGAGATTGGCAATCTTCCTTGTAATCTTAGCCAGCAATTTGGCATCATCGTCGCCGGTTCCAGTCGATAAGTCGCAACTAAACAAATGGTTTTCCGACAGTATCAAGCCGCTGGACGCTCGCACAGAGCTTGATAAAGCCGTGGTCGAGGCCGAGAAAGACGCGACCATGGTGAAAGTAAGGGCAGACGGGAGTGGAAATTTCAAGACGATAACGGAAGCCATAAACAGCGTTCCAGCCAACAACAAAAAACGCGTACTCATTTGGATTGGAGGAGGAGTTTACAAAGAGAAGCTTAAGATTGAAATAAATAAACCATTCATTACTTTTTATGGATCTCCTAACGATATGCCAACTTTGACTTTTGATGGTGATTCCAAAACTTTCGGAACCGTTGACAGCGGCACTTTGACTGTCGAATCTGATTATTTTGTTGCTGCCAATATCATCCTTAAGAATTCATCCCCAAGGCCAAACAAAGAGGGAGGAGGACCGGCATTAGCGGCAAGAATAATGGGAAACAAAGCCGCAATTTACAATTGCAAATTCTATGGGTTTCAAGACACCCTTTGCGACGACGCCGGCATGCATCTTTACAAAGATTGCTTCATTCAAGGCACCACAGACTTCATTTTTGGGAGAGGAACCTCCCTTTATTTGAACTGTCAATTGGATGTGGTGAAAGAGGGTTCGGTGATCGCAGCACATTCGAGAGAGCTTGAACATGACGGAAGCGGCTACTCGTTTGTGCATTGCACCATTACCGGACCGGATACGAAGAGAATTTATTTGGGAAGAGCTTGGAGGCCTAGGTCTAGAGTTGTATACTCCTACACCAAAATGGCAGACAATGTTCACCCTGAAGGCTGGCACGACATGGGAAATCCCAAATACACTTCCTCGATTTTGTTCGGAGAATACAAGTGTTCAGGGCCGGGATCACAAACGGATCAGCGCGTATCCTACAGTAAACGATTGAGTGACGCAGAAGCGAAGCCATACCTTAGCCTTAATTACATAGAGGCTGCCAAATGGTTGCTTCCTCCTCCCAGCCTAAAGGCTTAA

Coding sequence (CDS)

ATGAAATATTACTTTGTAACGAGATTGGCAATCTTCCTTGTAATCTTAGCCAGCAATTTGGCATCATCGTCGCCGGTTCCAGTCGATAAGTCGCAACTAAACAAATGGTTTTCCGACAGTATCAAGCCGCTGGACGCTCGCACAGAGCTTGATAAAGCCGTGGTCGAGGCCGAGAAAGACGCGACCATGGTGAAAGTAAGGGCAGACGGGAGTGGAAATTTCAAGACGATAACGGAAGCCATAAACAGCGTTCCAGCCAACAACAAAAAACGCGTACTCATTTGGATTGGAGGAGGAGTTTACAAAGAGAAGCTTAAGATTGAAATAAATAAACCATTCATTACTTTTTATGGATCTCCTAACGATATGCCAACTTTGACTTTTGATGGTGATTCCAAAACTTTCGGAACCGTTGACAGCGGCACTTTGACTGTCGAATCTGATTATTTTGTTGCTGCCAATATCATCCTTAAGAATTCATCCCCAAGGCCAAACAAAGAGGGAGGAGGACCGGCATTAGCGGCAAGAATAATGGGAAACAAAGCCGCAATTTACAATTGCAAATTCTATGGGTTTCAAGACACCCTTTGCGACGACGCCGGCATGCATCTTTACAAAGATTGCTTCATTCAAGGCACCACAGACTTCATTTTTGGGAGAGGAACCTCCCTTTATTTGAACTGTCAATTGGATGTGGTGAAAGAGGGTTCGGTGATCGCAGCACATTCGAGAGAGCTTGAACATGACGGAAGCGGCTACTCGTTTGTGCATTGCACCATTACCGGACCGGATACGAAGAGAATTTATTTGGGAAGAGCTTGGAGGCCTAGGTCTAGAGTTGTATACTCCTACACCAAAATGGCAGACAATGTTCACCCTGAAGGCTGGCACGACATGGGAAATCCCAAATACACTTCCTCGATTTTGTTCGGAGAATACAAGTGTTCAGGGCCGGGATCACAAACGGATCAGCGCGTATCCTACAGTAAACGATTGAGTGACGCAGAAGCGAAGCCATACCTTAGCCTTAATTACATAGAGGCTGCCAAATGGTTGCTTCCTCCTCCCAGCCTAAAGGCTTAA

Protein sequence

MKYYFVTRLAIFLVILASNLASSSPVPVDKSQLNKWFSDSIKPLDARTELDKAVVEAEKDATMVKVRADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGSPNDMPTLTFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPNKEGGGPALAARIMGNKAAIYNCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEGSVIAAHSRELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGNPKYTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPPSLKA

Homology

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BLAST of Sed0022128 vs. NCBI nr
Match: XP_022152989.1 (putative pectinesterase 63 [Momordica charantia])

HSP 1 Score: 503.8 bits (1296), Expect = 1.2e-138
Identity = 246/359 (68.52%), Postives = 290/359 (80.78%), Query Frame = 0


Query: 6   VTRLAIFLVILASNLASSSPVPVDKSQLNKWFSDSIKPL-DARTELDKAVVEAEKDATMV 65
           +TRL   L   A  LA+S PVP DKSQ+NKWFSD++KPL D + ELD A+V AE+ AT+V
Sbjct: 10  LTRLLTLLATFALILAASPPVPADKSQVNKWFSDNVKPLADRKAELDPALVAAEEGATVV 69

Query: 66  KVRADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGSPNDMP 125
           KVRADGSG+FKT+TEAINSVP  N KRV+IWIGGGVYKEKLKIE  KPF+T YGSPN+MP
Sbjct: 70  KVRADGSGDFKTVTEAINSVPTGNTKRVVIWIGGGVYKEKLKIERTKPFVTLYGSPNNMP 129

Query: 126 TLTFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPN-KEGGGPALAARIMGNKAA 185
            LTFDGD+  FGTV S TLTVESDYF AAN+I+ NSSPRP+ K  G  ALAAR+MG KAA
Sbjct: 130 NLTFDGDANKFGTVYSATLTVESDYFTAANLIIVNSSPRPDGKRKGAQALAARLMGTKAA 189

Query: 186 IYNCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG--SVIAA 245
           +YNCKF GFQDTLCDD G+HLYKDCFIQGT DFIFG+GTSLYLN QLDVV +G  +VI A
Sbjct: 190 VYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSLYLNSQLDVVGDGGLAVITA 249

Query: 246 HSRELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGN 305
           HSRE E D SGYSFVH +ITG   K  YLGRAW  RSR V++YT MAD ++PEGW+DM +
Sbjct: 250 HSREEESDPSGYSFVHGSITGTGGKNTYLGRAWMARSRTVFAYTTMADIINPEGWNDMKH 309

Query: 306 PKYTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPPSLKA 361
           P + S++LFGEYKCSGPGS+   RV YSK+LSD E KP++SL ++++ KWLLPPP+ +A
Sbjct: 310 PGFDSTVLFGEYKCSGPGSEPSGRVKYSKQLSDVEVKPFVSLGFLQSDKWLLPPPNPEA 368

[Close]

BLAST of Sed0022128 vs. NCBI nr
Match: XP_038902684.1 (putative pectinesterase 63 [Benincasa hispida])

HSP 1 Score: 499.6 bits (1285), Expect = 2.3e-137
Identity = 239/356 (67.13%), Postives = 288/356 (80.90%), Query Frame = 0


Query: 6   VTRLAIFLVILASNLASSSPVPVDKSQLNKWFSDSIKPL-DARTELDKAVVEAEKDATMV 65
           +TRL IFL   A+  A S PVP +KSQL  WFS+++KP  D + ELD A+V AE++AT+V
Sbjct: 10  LTRLVIFLAAFAAIFAVSPPVPAEKSQLEGWFSENVKPFADCKAELDPALVAAEENATVV 69

Query: 66  KVRADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGSPNDMP 125
           KVRADGSG+FKT+TEAINSVPA N +RV+IWIG GVYKEKLKI+ NKPF+T YGSPN+MP
Sbjct: 70  KVRADGSGDFKTVTEAINSVPAGNTRRVVIWIGEGVYKEKLKIDRNKPFVTLYGSPNNMP 129

Query: 126 TLTFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPNK-EGGGPALAARIMGNKAA 185
            LTFDGD+  +GTV S TL VESDYFVAAN++++NSSPRP+    G  ALAAR+MG KAA
Sbjct: 130 NLTFDGDAAKYGTVYSATLIVESDYFVAANLVIENSSPRPDGIRKGAQALAARLMGTKAA 189

Query: 186 IYNCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG--SVIAA 245
           IYNCKF GFQDTLCDD G+HLYKDCFIQGT DFIFG+GTSLYLN +LDVV +G  +VI A
Sbjct: 190 IYNCKFIGFQDTLCDDEGLHLYKDCFIQGTVDFIFGKGTSLYLNSRLDVVGDGGLAVITA 249

Query: 246 HSRELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGN 305
           HSRE E D SGYSF HC+ITG   K  YLGRAW PRSRVV++YT MAD +H EGW+DM +
Sbjct: 250 HSREEESDSSGYSFAHCSITGTGGKNTYLGRAWMPRSRVVFAYTTMADIIHAEGWNDMNH 309

Query: 306 PKYTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPPS 358
             Y +++LFGEYKCSGPG++   RV YSK+LSD E KP++SL Y+++ KWLLPPP+
Sbjct: 310 SGYDATVLFGEYKCSGPGAELSGRVKYSKQLSDVEVKPFISLGYLQSEKWLLPPPN 365

[Close]

BLAST of Sed0022128 vs. NCBI nr
Match: XP_022944181.1 (pectinesterase PPME1-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 493.0 bits (1268), Expect = 2.1e-135
Identity = 235/356 (66.01%), Postives = 287/356 (80.62%), Query Frame = 0


Query: 6   VTRLAIFLVILASNLASSSPVPVDKSQLNKWFSDSIKPL-DARTELDKAVVEAEKDATMV 65
           +T L IFL   A   ++++ VP +KSQL  WFS  +KPL D + ELD A+V AE+ +T+V
Sbjct: 5   LTTLLIFLAAFAPISSATTEVPAEKSQLEAWFSGHVKPLADRKAELDPALVAAEESSTVV 64

Query: 66  KVRADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGSPNDMP 125
           KVRADGSG+FKT+TEAI SVPA N KRV+IWIG GVYKEKL IE NKPFIT YGSPN+MP
Sbjct: 65  KVRADGSGDFKTVTEAIASVPAGNTKRVVIWIGKGVYKEKLTIEKNKPFITLYGSPNNMP 124

Query: 126 TLTFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPNKEGGGPALAARIMGNKAAI 185
           TLTFDGD+K FGTV SGTLTVES+YFVAAN++++N+SPRP+   GGPALAARI  NKAAI
Sbjct: 125 TLTFDGDAKKFGTVYSGTLTVESEYFVAANLVIENTSPRPHAGKGGPALAARIRANKAAI 184

Query: 186 YNCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG--SVIAAH 245
           YNCKF GFQDTLCDD GMH+YKDC I+GT DFIFG+ T+LYLN QL+V+  G   VIAAH
Sbjct: 185 YNCKFLGFQDTLCDDDGMHVYKDCVIEGTVDFIFGKATALYLNTQLNVLGVGGLGVIAAH 244

Query: 246 SRELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGNP 305
           SRE E D SGY F HC+ITG      YLGR+WRP SRVV++YT +AD +HPEGW DM NP
Sbjct: 245 SREKEDDPSGYVFAHCSITGTGGPNTYLGRSWRPWSRVVFAYTTIADILHPEGWDDMNNP 304

Query: 306 KYTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPPSL 359
            Y  ++ FGEYKCSGPG++T +R+ +SK+LS+A+AKP+++L+Y++A KWLLPPP L
Sbjct: 305 AYDKTVFFGEYKCSGPGAETSKRIKFSKQLSEAQAKPFINLDYVQAKKWLLPPPKL 360

[Close]

BLAST of Sed0022128 vs. NCBI nr
Match: KAG6570448.1 (Pectinesterase PPME1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 490.0 bits (1260), Expect = 1.8e-134
Identity = 233/345 (67.54%), Postives = 281/345 (81.45%), Query Frame = 0


Query: 17  ASNLASSSPVPVDKSQLNKWFSDSIKPLDAR-TELDKAVVEAEKDATMVKVRADGSGNFK 76
           A++ + SS VP +KSQL  WFS+++KPL AR  ELD A+V AE++AT+VKVRADGSG+FK
Sbjct: 70  ATSASPSSEVPAEKSQLEAWFSENVKPLSARKAELDPALVAAEENATVVKVRADGSGDFK 129

Query: 77  TITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGSPNDMPTLTFDGDSKTF 136
           T+TEAI SVPA N  RV+IWIG GVYKEKL IE NKPFIT YGSP +MP LTFDGDSK +
Sbjct: 130 TVTEAIASVPAGNTHRVVIWIGEGVYKEKLTIERNKPFITLYGSPKNMPKLTFDGDSKKY 189

Query: 137 GTVDSGTLTVESDYFVAANIILKNSSPRPNKEGGGPALAARIMGNKAAIYNCKFYGFQDT 196
           GTV SGTLT+E++YFVAAN++++N+SPRP+   GGPALAARI GNKAAIYNCKF GFQDT
Sbjct: 190 GTVYSGTLTMEAEYFVAANLVIENTSPRPDARKGGPALAARIRGNKAAIYNCKFIGFQDT 249

Query: 197 LCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG--SVIAAHSRELEHDGSGY 256
           LCDD GMH+YKDCFI+GT DFIFG+ TSLYLN QL+VV EG   VIAAHSRE E D SGY
Sbjct: 250 LCDDDGMHVYKDCFIEGTVDFIFGKATSLYLNSQLNVVGEGGLGVIAAHSREKEDDPSGY 309

Query: 257 SFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGNPKYTSSILFGEY 316
            F HC+ITG      YL R+WRP SRVV++YT +AD +HPEGW DM +P +  ++ FGEY
Sbjct: 310 VFAHCSITGTGGPNTYLARSWRPWSRVVFAYTTIADVLHPEGWDDMNHPNFDKTVFFGEY 369

Query: 317 KCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPPSL 359
           K SGPG++T  R+ +SK+LSDAE KP+LSL+Y++A KWLLPPP L
Sbjct: 370 KSSGPGAETSSRIKFSKQLSDAEVKPFLSLDYVQAQKWLLPPPKL 414

[Close]

BLAST of Sed0022128 vs. NCBI nr
Match: XP_022944179.1 (pectinesterase PPME1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 489.6 bits (1259), Expect = 2.4e-134
Identity = 233/345 (67.54%), Postives = 281/345 (81.45%), Query Frame = 0


Query: 17  ASNLASSSPVPVDKSQLNKWFSDSIKPLDAR-TELDKAVVEAEKDATMVKVRADGSGNFK 76
           A++ + SS VP +KSQL  WFS+++KPL AR  ELD A+V AE++AT+VKVRADGSG+FK
Sbjct: 70  ATSASPSSEVPAEKSQLEAWFSENVKPLSARKAELDPALVAAEENATVVKVRADGSGDFK 129

Query: 77  TITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGSPNDMPTLTFDGDSKTF 136
           T+TEAI SVPA N  RV+IWIG GVYKEKL IE NKPFIT YGSP +MP LTFDGDSK +
Sbjct: 130 TVTEAIASVPAGNTHRVVIWIGEGVYKEKLTIERNKPFITLYGSPKNMPKLTFDGDSKKY 189

Query: 137 GTVDSGTLTVESDYFVAANIILKNSSPRPNKEGGGPALAARIMGNKAAIYNCKFYGFQDT 196
           GTV SGTLT+E++YFVAAN++++N+SPRP+   GGPALAARI GNKAAIYNCKF GFQDT
Sbjct: 190 GTVYSGTLTMEAEYFVAANLVIENTSPRPDARKGGPALAARIRGNKAAIYNCKFIGFQDT 249

Query: 197 LCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG--SVIAAHSRELEHDGSGY 256
           LCDD GMH+YKDCFI+GT DFIFG+ TSLYLN QL+VV EG   VIAAHSRE E D SGY
Sbjct: 250 LCDDDGMHVYKDCFIEGTVDFIFGKATSLYLNSQLNVVGEGGLGVIAAHSREKEDDPSGY 309

Query: 257 SFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGNPKYTSSILFGEY 316
            F HC+ITG      YL R+WRP SRVV++YT +AD +HPEGW DM +P +  ++ FGEY
Sbjct: 310 VFAHCSITGTGGPNTYLARSWRPWSRVVFAYTTIADVLHPEGWDDMNHPDFDKTVFFGEY 369

Query: 317 KCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPPSL 359
           K SGPG++T  R+ +SK+LSDAE KP+LSL+Y++A KWLLPPP L
Sbjct: 370 KSSGPGAETSSRIKFSKQLSDAEVKPFLSLDYVQAQKWLLPPPKL 414

[Close]

BLAST of Sed0022128 vs. ExPASy Swiss-Prot
Match: Q9LY19 (Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2)

HSP 1 Score: 367.5 bits (942), Expect = 1.8e-100
Identity = 184/363 (50.69%), Postives = 246/363 (67.77%), Query Frame = 0


Query: 1   MKYYFVTRLAIFLVILASNLASS---SPVPVDKSQLNKWFSDSIKPLDARTELDKAVVEA 60
           M+Y  V+ L   LVI  S +  +   +P+P  K Q+ +WF+  + PL  R  LD A+V A
Sbjct: 1   MRYTNVSILLGMLVIFVSPMVFADDLTPIPEGKPQVVQWFNTHVGPLAQRKGLDPALVAA 60

Query: 61  EKDATMVKVRADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFY 120
           E    ++ V   G G FKT+T+AI SVPA N KRV+I +  G Y+EK+ I+ NKPFIT  
Sbjct: 61  EAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFITLM 120

Query: 121 GSPNDMPTLTFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPN-KEGGGPALAAR 180
           G PN MP +T+DG +  +GTVDS +L + SDYF+A NI++KN++P P+ K  G  AL+ R
Sbjct: 121 GQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMR 180

Query: 181 IMGNKAAIYNCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG 240
           I GN AA YNCKFYGFQDT+CDD G H +KDC+++GT DFIFG GTS+YL  QL VV +G
Sbjct: 181 ISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDG 240

Query: 241 -SVIAAHSRELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEG 300
             VIAAH+ +   + SGYSFVHC +TG     IYLGRAW    +VVY+YT+M   V+P G
Sbjct: 241 IRVIAAHAGKSAEEKSGYSFVHCKVTGTG-GGIYLGRAWMSHPKVVYAYTEMTSVVNPTG 300

Query: 301 WHDMGNPKYTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPP 359
           W +   P +  ++ +GEYKCSGPGS   +RV +++ + D EA  +LSL YI+ +KWLLPP
Sbjct: 301 WQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWLLPP 360

[Close]

BLAST of Sed0022128 vs. ExPASy Swiss-Prot
Match: Q84WM7 (Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.8e-100
Identity = 177/337 (52.52%), Postives = 235/337 (69.73%), Query Frame = 0


Query: 24  SPVPVDKSQLNKWFSDSIKPLDARTELDKAVVEAEKDATMVKVRADGSGNFKTITEAINS 83
           +P+P  K Q+ +WF+ ++ PL  R  LD A+V AE    ++ V   G G FKT+T+AI S
Sbjct: 27  TPIPEGKPQVAQWFNANVGPLAQRKGLDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKS 86

Query: 84  VPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGSPNDMPTLTFDGDSKTFGTVDSGTL 143
           VPA N KRV+I +  G YKEK+ I+ NKPFIT  G PN MP +T+DG +  +GTVDS +L
Sbjct: 87  VPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMGQPNAMPVITYDGTAAKYGTVDSASL 146

Query: 144 TVESDYFVAANIILKNSSPRPN-KEGGGPALAARIMGNKAAIYNCKFYGFQDTLCDDAGM 203
            + SDYF+A NI++KN++P P+ K  G  AL+ RI GN AA YNCKFYGFQDT+CDD G 
Sbjct: 147 IILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGN 206

Query: 204 HLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG-SVIAAHSRELEHDGSGYSFVHCTIT 263
           H +KDC+++GT DFIFG GTS+YL  QL VV +G  VIAAH+ +   + SGYSFVHC +T
Sbjct: 207 HFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDGIRVIAAHAGKSAEEKSGYSFVHCKVT 266

Query: 264 GPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGNPKYTSSILFGEYKCSGPGSQ 323
           G     IYLGRAW    +VVY+YT+M   V+P GW +   P +  ++ +GEYKCSGPGS 
Sbjct: 267 GTG-GGIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSH 326

Query: 324 TDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPPSL 359
             +RV +++ + D EA  +LSL YI+ +KWLLPPP+L
Sbjct: 327 KAKRVPFTQDIDDKEANRFLSLGYIQGSKWLLPPPAL 361

[Close]

BLAST of Sed0022128 vs. ExPASy Swiss-Prot
Match: Q9FKF3 (Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2)

HSP 1 Score: 357.8 bits (917), Expect = 1.4e-97
Identity = 182/363 (50.14%), Postives = 241/363 (66.39%), Query Frame = 0


Query: 1   MKYYFVTRL-AIFLVILASNLA---SSSPVPVDKSQLNKWFSDSIKPLDARTELDKAVVE 60
           M Y +V+ +  I LV++ S +     ++P+P +K ++ +WF+ ++K              
Sbjct: 1   MGYNYVSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTNVK-------------- 60

Query: 61  AEKDATMVKVRADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITF 120
                       +G G+FKTITEAINSV A N +RV+I IG GVYKEK+ I+ +KPFIT 
Sbjct: 61  -----------QNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITL 120

Query: 121 YGSPNDMPTLTFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPN-KEGGGPALAA 180
           YG PN MP LTFDG +  +GTVDS TL V SDYF+A NIILKNS+P P+ K  G  AL+ 
Sbjct: 121 YGHPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSM 180

Query: 181 RIMGNKAAIYNCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKE 240
           RI GNKAA YNCKFYG+QDT+CDD G H +KDC+I+GT DFIFG G SLYL  QL+VV +
Sbjct: 181 RISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGD 240

Query: 241 G-SVIAAHSRELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPE 300
           G  VI AH+ +   + SGYSFVHC +TG  T  IYLGR+W    +VVY+YT M+  V+P 
Sbjct: 241 GIRVITAHAGKSAAEKSGYSFVHCKVTGTGT-GIYLGRSWMSHPKVVYAYTDMSSVVNPS 300

Query: 301 GWHDMGNPKYTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLP 358
           GW +        ++ +GEYKC+G GS  ++RV Y++ + D EAK ++SL YI+ + WLLP
Sbjct: 301 GWQENREAGRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLP 337

[Close]

BLAST of Sed0022128 vs. ExPASy Swiss-Prot
Match: B2VPR8 (Pectinesterase 2 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 6.1e-93
Identity = 173/352 (49.15%), Postives = 238/352 (67.61%), Query Frame = 0


Query: 9   LAIFLVILASNLASSSPVPVDKSQLNKWFSDSIKPLDAR-TELDKAVVEAEKDATMVKVR 68
           L I L I        +P+P + +QLN WF   I+P+  R   +D A+V AE  A ++K++
Sbjct: 11  LGILLYIPIVLSDDRAPIPANSAQLNSWFDGIIQPVAVRKATMDPALVTAEGQAKVIKLK 70

Query: 69  ADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYG-SPNDMPTL 128
           +DGSG+FK+I EAI S+P +N KRV++    G Y EK+KI + K +ITFYG  PN+MP L
Sbjct: 71  SDGSGDFKSINEAIKSIPDDNTKRVILSFSPGNYSEKVKIGMYKHYITFYGEDPNNMPIL 130

Query: 129 TFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPN-KEGGGPALAARIMGNKAAIY 188
            F G +  +GTVDS TL VES+YF A N+ + NS+PRP+ K  G  A A RI G+KA+ Y
Sbjct: 131 VFGGTAAEYGTVDSATLIVESNYFSAVNLKIVNSAPRPDGKRVGAQAAALRISGDKASFY 190

Query: 189 NCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVV--KEGSVIAAHS 248
           N K YGFQDTLCDD G H YKDC+I+GT DFIFG G S++LN +L  V   + ++I A +
Sbjct: 191 NVKIYGFQDTLCDDKGKHFYKDCYIEGTVDFIFGSGKSIFLNTELHAVPGDQPAIITAQA 250

Query: 249 RELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGNPK 308
           R+ E + +GY FV+C +TG      +LGR+W P ++VV++YT+M D +HPEGW  +  P+
Sbjct: 251 RKTESEDTGYYFVNCRVTGGGA---FLGRSWMPAAKVVFAYTEMGDAIHPEGW-ILVKPE 310

Query: 309 YTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPP 356
           + S++ F EY   GPG+  ++R  + KRLSDAEAK  +SL  IEA+KWLLPP
Sbjct: 311 HESTVRFPEYNNKGPGANMEKRAKFVKRLSDAEAKQSISLGSIEASKWLLPP 358

[Close]

BLAST of Sed0022128 vs. ExPASy Swiss-Prot
Match: D8VPP5 (Pectinesterase 1 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.3e-92
Identity = 171/352 (48.58%), Postives = 238/352 (67.61%), Query Frame = 0


Query: 9   LAIFLVILASNLASSSPVPVDKSQLNKWFSDSIKPLDAR-TELDKAVVEAEKDATMVKVR 68
           L I L I        +P+P + +QLN WF   I+P+  R   +D A+V AE    ++K++
Sbjct: 11  LGILLYIPIVLSDDRAPIPSNSAQLNSWFDGIIQPVAVRKATMDPALVTAEGQTKVIKLK 70

Query: 69  ADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYG-SPNDMPTL 128
           +DGSG+FK+I EAI S+P +N KRV++ +  G Y EK+KI + K +ITFYG  PN+MP L
Sbjct: 71  SDGSGDFKSINEAIKSIPDDNTKRVILSLAPGNYSEKVKIGMYKHYITFYGEDPNNMPIL 130

Query: 129 TFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPN-KEGGGPALAARIMGNKAAIY 188
            F G +  +GTVDS TL VES+YF A N+ + NS+PRP+ K  G  A A RI G+KA+ Y
Sbjct: 131 VFGGTAAEYGTVDSATLIVESNYFSAVNLKIVNSAPRPDGKRVGAQAAALRISGDKASFY 190

Query: 189 NCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVV--KEGSVIAAHS 248
           N K YGFQDTLCDD G H YKDC+I+GT DFIFG G S++LN +L  V   + ++I A +
Sbjct: 191 NVKIYGFQDTLCDDKGKHFYKDCYIEGTVDFIFGSGKSIFLNTELHAVPGDQPAIITAQA 250

Query: 249 RELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGNPK 308
           R+ + + +GY FV+C +TG      +LGR+W P ++VV++YT+M D +HPEGW  +  P+
Sbjct: 251 RKTDSEDTGYYFVNCRVTGGGA---FLGRSWMPAAKVVFAYTEMVDAIHPEGW-ILVKPE 310

Query: 309 YTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPP 356
           + S++ F EY   GPG+  ++R  + KRLSDAEAK  +SL  IEA+KWLLPP
Sbjct: 311 HESTVRFSEYNNKGPGANMEKRAKFVKRLSDAEAKQSISLGSIEASKWLLPP 358

[Close]

BLAST of Sed0022128 vs. ExPASy TrEMBL
Match: A0A6J1DHP7 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111020595 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 5.8e-139
Identity = 246/359 (68.52%), Postives = 290/359 (80.78%), Query Frame = 0


Query: 6   VTRLAIFLVILASNLASSSPVPVDKSQLNKWFSDSIKPL-DARTELDKAVVEAEKDATMV 65
           +TRL   L   A  LA+S PVP DKSQ+NKWFSD++KPL D + ELD A+V AE+ AT+V
Sbjct: 10  LTRLLTLLATFALILAASPPVPADKSQVNKWFSDNVKPLADRKAELDPALVAAEEGATVV 69

Query: 66  KVRADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGSPNDMP 125
           KVRADGSG+FKT+TEAINSVP  N KRV+IWIGGGVYKEKLKIE  KPF+T YGSPN+MP
Sbjct: 70  KVRADGSGDFKTVTEAINSVPTGNTKRVVIWIGGGVYKEKLKIERTKPFVTLYGSPNNMP 129

Query: 126 TLTFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPN-KEGGGPALAARIMGNKAA 185
            LTFDGD+  FGTV S TLTVESDYF AAN+I+ NSSPRP+ K  G  ALAAR+MG KAA
Sbjct: 130 NLTFDGDANKFGTVYSATLTVESDYFTAANLIIVNSSPRPDGKRKGAQALAARLMGTKAA 189

Query: 186 IYNCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG--SVIAA 245
           +YNCKF GFQDTLCDD G+HLYKDCFIQGT DFIFG+GTSLYLN QLDVV +G  +VI A
Sbjct: 190 VYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSLYLNSQLDVVGDGGLAVITA 249

Query: 246 HSRELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGN 305
           HSRE E D SGYSFVH +ITG   K  YLGRAW  RSR V++YT MAD ++PEGW+DM +
Sbjct: 250 HSREEESDPSGYSFVHGSITGTGGKNTYLGRAWMARSRTVFAYTTMADIINPEGWNDMKH 309

Query: 306 PKYTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPPSLKA 361
           P + S++LFGEYKCSGPGS+   RV YSK+LSD E KP++SL ++++ KWLLPPP+ +A
Sbjct: 310 PGFDSTVLFGEYKCSGPGSEPSGRVKYSKQLSDVEVKPFVSLGFLQSDKWLLPPPNPEA 368

[Close]

BLAST of Sed0022128 vs. ExPASy TrEMBL
Match: A0A6J1FTQ2 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111448705 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 1.0e-135
Identity = 235/356 (66.01%), Postives = 287/356 (80.62%), Query Frame = 0


Query: 6   VTRLAIFLVILASNLASSSPVPVDKSQLNKWFSDSIKPL-DARTELDKAVVEAEKDATMV 65
           +T L IFL   A   ++++ VP +KSQL  WFS  +KPL D + ELD A+V AE+ +T+V
Sbjct: 5   LTTLLIFLAAFAPISSATTEVPAEKSQLEAWFSGHVKPLADRKAELDPALVAAEESSTVV 64

Query: 66  KVRADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGSPNDMP 125
           KVRADGSG+FKT+TEAI SVPA N KRV+IWIG GVYKEKL IE NKPFIT YGSPN+MP
Sbjct: 65  KVRADGSGDFKTVTEAIASVPAGNTKRVVIWIGKGVYKEKLTIEKNKPFITLYGSPNNMP 124

Query: 126 TLTFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPNKEGGGPALAARIMGNKAAI 185
           TLTFDGD+K FGTV SGTLTVES+YFVAAN++++N+SPRP+   GGPALAARI  NKAAI
Sbjct: 125 TLTFDGDAKKFGTVYSGTLTVESEYFVAANLVIENTSPRPHAGKGGPALAARIRANKAAI 184

Query: 186 YNCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG--SVIAAH 245
           YNCKF GFQDTLCDD GMH+YKDC I+GT DFIFG+ T+LYLN QL+V+  G   VIAAH
Sbjct: 185 YNCKFLGFQDTLCDDDGMHVYKDCVIEGTVDFIFGKATALYLNTQLNVLGVGGLGVIAAH 244

Query: 246 SRELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGNP 305
           SRE E D SGY F HC+ITG      YLGR+WRP SRVV++YT +AD +HPEGW DM NP
Sbjct: 245 SREKEDDPSGYVFAHCSITGTGGPNTYLGRSWRPWSRVVFAYTTIADILHPEGWDDMNNP 304

Query: 306 KYTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPPSL 359
            Y  ++ FGEYKCSGPG++T +R+ +SK+LS+A+AKP+++L+Y++A KWLLPPP L
Sbjct: 305 AYDKTVFFGEYKCSGPGAETSKRIKFSKQLSEAQAKPFINLDYVQAKKWLLPPPKL 360

[Close]

BLAST of Sed0022128 vs. ExPASy TrEMBL
Match: A0A6J1FW87 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111448705 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 1.1e-134
Identity = 233/345 (67.54%), Postives = 281/345 (81.45%), Query Frame = 0


Query: 17  ASNLASSSPVPVDKSQLNKWFSDSIKPLDAR-TELDKAVVEAEKDATMVKVRADGSGNFK 76
           A++ + SS VP +KSQL  WFS+++KPL AR  ELD A+V AE++AT+VKVRADGSG+FK
Sbjct: 70  ATSASPSSEVPAEKSQLEAWFSENVKPLSARKAELDPALVAAEENATVVKVRADGSGDFK 129

Query: 77  TITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGSPNDMPTLTFDGDSKTF 136
           T+TEAI SVPA N  RV+IWIG GVYKEKL IE NKPFIT YGSP +MP LTFDGDSK +
Sbjct: 130 TVTEAIASVPAGNTHRVVIWIGEGVYKEKLTIERNKPFITLYGSPKNMPKLTFDGDSKKY 189

Query: 137 GTVDSGTLTVESDYFVAANIILKNSSPRPNKEGGGPALAARIMGNKAAIYNCKFYGFQDT 196
           GTV SGTLT+E++YFVAAN++++N+SPRP+   GGPALAARI GNKAAIYNCKF GFQDT
Sbjct: 190 GTVYSGTLTMEAEYFVAANLVIENTSPRPDARKGGPALAARIRGNKAAIYNCKFIGFQDT 249

Query: 197 LCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG--SVIAAHSRELEHDGSGY 256
           LCDD GMH+YKDCFI+GT DFIFG+ TSLYLN QL+VV EG   VIAAHSRE E D SGY
Sbjct: 250 LCDDDGMHVYKDCFIEGTVDFIFGKATSLYLNSQLNVVGEGGLGVIAAHSREKEDDPSGY 309

Query: 257 SFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGNPKYTSSILFGEY 316
            F HC+ITG      YL R+WRP SRVV++YT +AD +HPEGW DM +P +  ++ FGEY
Sbjct: 310 VFAHCSITGTGGPNTYLARSWRPWSRVVFAYTTIADVLHPEGWDDMNHPDFDKTVFFGEY 369

Query: 317 KCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPPSL 359
           K SGPG++T  R+ +SK+LSDAE KP+LSL+Y++A KWLLPPP L
Sbjct: 370 KSSGPGAETSSRIKFSKQLSDAEVKPFLSLDYVQAQKWLLPPPKL 414

[Close]

BLAST of Sed0022128 vs. ExPASy TrEMBL
Match: A0A6J1J8V7 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483650 PE=4 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.3e-133
Identity = 231/356 (64.89%), Postives = 286/356 (80.34%), Query Frame = 0


Query: 6   VTRLAIFLVILASNLASSSPVPVDKSQLNKWFSDSIKPLDAR-TELDKAVVEAEKDATMV 65
           ++ L IFL   A   ++++ VP +KSQL  WFS  +KPL AR  ELD A+V AE+ +T+V
Sbjct: 5   LSALVIFLAAFAPISSATTEVPAEKSQLEAWFSGHVKPLAARKAELDPALVAAEESSTVV 64

Query: 66  KVRADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGSPNDMP 125
           KVRADGSG+FKT+TEAI SVPA N KRV+IWIG GVYKEKL IE NKPFIT YGSPN+MP
Sbjct: 65  KVRADGSGDFKTVTEAIASVPAGNTKRVVIWIGEGVYKEKLTIERNKPFITLYGSPNNMP 124

Query: 126 TLTFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPNKEGGGPALAARIMGNKAAI 185
            LTFDGD+K +GTV S TL VE++YFVAAN++++N+SPRP+   GGPALAARI GNKAAI
Sbjct: 125 NLTFDGDAKKYGTVYSATLIVEAEYFVAANLVIENTSPRPDARKGGPALAARIRGNKAAI 184

Query: 186 YNCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG--SVIAAH 245
           YNCKF GFQDTLCDD G+H+YKDCFIQGT DFIFG+ T+LYLN QL+V+  G   VIAAH
Sbjct: 185 YNCKFIGFQDTLCDDDGLHVYKDCFIQGTVDFIFGKATALYLNSQLNVLGVGGLGVIAAH 244

Query: 246 SRELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGNP 305
           SRE E D SGY F HC+ITG      YLGR+WRP SRVV++YT +AD +HPEGW DM NP
Sbjct: 245 SREKEDDPSGYVFAHCSITGTGGPNTYLGRSWRPWSRVVFAYTTIADILHPEGWDDMNNP 304

Query: 306 KYTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPPSL 359
            +  ++ FGE+KCSGPG++T +RV +SK+LS+A+AKP+++L+Y++A KWLLPPP L
Sbjct: 305 AFDKTVFFGEFKCSGPGAETSKRVKFSKQLSEAQAKPFINLDYVQAQKWLLPPPKL 360

[Close]

BLAST of Sed0022128 vs. ExPASy TrEMBL
Match: A0A5A7V7V3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00480 PE=4 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 3.7e-133
Identity = 231/356 (64.89%), Postives = 285/356 (80.06%), Query Frame = 0


Query: 6   VTRLAIFLVILASNLASSSPVPVDKSQLNKWFSDSIKPL-DARTELDKAVVEAEKDATMV 65
           +TRL  FL    + LA S+P+PV+KSQ++ WFS ++KPL D + ELD A+V AE++AT++
Sbjct: 10  ITRLVTFLATFTTILAVSTPIPVEKSQIDAWFSQNVKPLADRKAELDPALVAAEENATII 69

Query: 66  KVRADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGSPNDMP 125
           KV +DG+G+FKTITEAI SVPA+NKKRV+I IG G+YKEKLKI+ NKPF+T YGSP  MP
Sbjct: 70  KVMSDGTGDFKTITEAIASVPADNKKRVMISIGDGIYKEKLKIDRNKPFVTLYGSPKGMP 129

Query: 126 TLTFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPN-KEGGGPALAARIMGNKAA 185
            LTFDGD+  FGTV S TL VE+DYF AAN++++NSSPRP+    G  ALAAR MG KAA
Sbjct: 130 KLTFDGDAAKFGTVYSATLIVEADYFTAANLVIENSSPRPDGVRKGAQALAARFMGTKAA 189

Query: 186 IYNCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG--SVIAA 245
           IYNCKF GFQDTLCDD G+H YKDCFIQGT DFIFG+GTSLY+N QLDV  +G  +VI A
Sbjct: 190 IYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYVNTQLDVAGDGGLAVITA 249

Query: 246 HSRELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGN 305
           HSRE E DGSGYSFVHC+ITG   K  +LGRAW PRSRVV++YT MAD +HPEGW+DM +
Sbjct: 250 HSREQESDGSGYSFVHCSITGTGGKNTFLGRAWMPRSRVVFAYTTMADIIHPEGWNDMKH 309

Query: 306 PKYTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPPS 358
             +  +++FGEYKCSGPGS + +RV Y K+LSDAE  P+LSL Y+++ KWLLPPP+
Sbjct: 310 AGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDAEVNPFLSLAYVQSEKWLLPPPN 365

[Close]

BLAST of Sed0022128 vs. TAIR 10
Match: AT1G69940.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 367.5 bits (942), Expect = 1.3e-101
Identity = 177/337 (52.52%), Postives = 235/337 (69.73%), Query Frame = 0


Query: 24  SPVPVDKSQLNKWFSDSIKPLDARTELDKAVVEAEKDATMVKVRADGSGNFKTITEAINS 83
           +P+P  K Q+ +WF+ ++ PL  R  LD A+V AE    ++ V   G G FKT+T+AI S
Sbjct: 27  TPIPEGKPQVAQWFNANVGPLAQRKGLDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKS 86

Query: 84  VPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGSPNDMPTLTFDGDSKTFGTVDSGTL 143
           VPA N KRV+I +  G YKEK+ I+ NKPFIT  G PN MP +T+DG +  +GTVDS +L
Sbjct: 87  VPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMGQPNAMPVITYDGTAAKYGTVDSASL 146

Query: 144 TVESDYFVAANIILKNSSPRPN-KEGGGPALAARIMGNKAAIYNCKFYGFQDTLCDDAGM 203
            + SDYF+A NI++KN++P P+ K  G  AL+ RI GN AA YNCKFYGFQDT+CDD G 
Sbjct: 147 IILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGN 206

Query: 204 HLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG-SVIAAHSRELEHDGSGYSFVHCTIT 263
           H +KDC+++GT DFIFG GTS+YL  QL VV +G  VIAAH+ +   + SGYSFVHC +T
Sbjct: 207 HFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDGIRVIAAHAGKSAEEKSGYSFVHCKVT 266

Query: 264 GPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHDMGNPKYTSSILFGEYKCSGPGSQ 323
           G     IYLGRAW    +VVY+YT+M   V+P GW +   P +  ++ +GEYKCSGPGS 
Sbjct: 267 GTG-GGIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSH 326

Query: 324 TDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPPSL 359
             +RV +++ + D EA  +LSL YI+ +KWLLPPP+L
Sbjct: 327 KAKRVPFTQDIDDKEANRFLSLGYIQGSKWLLPPPAL 361

[Close]

BLAST of Sed0022128 vs. TAIR 10
Match: AT5G07410.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 367.5 bits (942), Expect = 1.3e-101
Identity = 184/363 (50.69%), Postives = 246/363 (67.77%), Query Frame = 0


Query: 1   MKYYFVTRLAIFLVILASNLASS---SPVPVDKSQLNKWFSDSIKPLDARTELDKAVVEA 60
           M+Y  V+ L   LVI  S +  +   +P+P  K Q+ +WF+  + PL  R  LD A+V A
Sbjct: 1   MRYTNVSILLGMLVIFVSPMVFADDLTPIPEGKPQVVQWFNTHVGPLAQRKGLDPALVAA 60

Query: 61  EKDATMVKVRADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFY 120
           E    ++ V   G G FKT+T+AI SVPA N KRV+I +  G Y+EK+ I+ NKPFIT  
Sbjct: 61  EAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFITLM 120

Query: 121 GSPNDMPTLTFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPN-KEGGGPALAAR 180
           G PN MP +T+DG +  +GTVDS +L + SDYF+A NI++KN++P P+ K  G  AL+ R
Sbjct: 121 GQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMR 180

Query: 181 IMGNKAAIYNCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG 240
           I GN AA YNCKFYGFQDT+CDD G H +KDC+++GT DFIFG GTS+YL  QL VV +G
Sbjct: 181 ISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDG 240

Query: 241 -SVIAAHSRELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEG 300
             VIAAH+ +   + SGYSFVHC +TG     IYLGRAW    +VVY+YT+M   V+P G
Sbjct: 241 IRVIAAHAGKSAEEKSGYSFVHCKVTGTG-GGIYLGRAWMSHPKVVYAYTEMTSVVNPTG 300

Query: 301 WHDMGNPKYTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPP 359
           W +   P +  ++ +GEYKCSGPGS   +RV +++ + D EA  +LSL YI+ +KWLLPP
Sbjct: 301 WQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWLLPP 360

[Close]

BLAST of Sed0022128 vs. TAIR 10
Match: AT5G61680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 357.8 bits (917), Expect = 9.9e-99
Identity = 182/363 (50.14%), Postives = 241/363 (66.39%), Query Frame = 0


Query: 1   MKYYFVTRL-AIFLVILASNLA---SSSPVPVDKSQLNKWFSDSIKPLDARTELDKAVVE 60
           M Y +V+ +  I LV++ S +     ++P+P +K ++ +WF+ ++K              
Sbjct: 1   MGYNYVSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTNVK-------------- 60

Query: 61  AEKDATMVKVRADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITF 120
                       +G G+FKTITEAINSV A N +RV+I IG GVYKEK+ I+ +KPFIT 
Sbjct: 61  -----------QNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITL 120

Query: 121 YGSPNDMPTLTFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPN-KEGGGPALAA 180
           YG PN MP LTFDG +  +GTVDS TL V SDYF+A NIILKNS+P P+ K  G  AL+ 
Sbjct: 121 YGHPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSM 180

Query: 181 RIMGNKAAIYNCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKE 240
           RI GNKAA YNCKFYG+QDT+CDD G H +KDC+I+GT DFIFG G SLYL  QL+VV +
Sbjct: 181 RISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGD 240

Query: 241 G-SVIAAHSRELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPE 300
           G  VI AH+ +   + SGYSFVHC +TG  T  IYLGR+W    +VVY+YT M+  V+P 
Sbjct: 241 GIRVITAHAGKSAAEKSGYSFVHCKVTGTGT-GIYLGRSWMSHPKVVYAYTDMSSVVNPS 300

Query: 301 GWHDMGNPKYTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLP 358
           GW +        ++ +GEYKC+G GS  ++RV Y++ + D EAK ++SL YI+ + WLLP
Sbjct: 301 GWQENREAGRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLP 337

[Close]

BLAST of Sed0022128 vs. TAIR 10
Match: AT5G07430.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 339.0 bits (868), Expect = 4.8e-93
Identity = 165/358 (46.09%), Postives = 230/358 (64.25%), Query Frame = 0


Query: 4   YFVTRLAIFLVILASNL---ASSSPVPVDKSQLNKWFSDSIKPLDART-ELDKAVVEAEK 63
           Y    +  FLV+ AS +     + P+P +++Q+ +WF  ++KP   R   LD A+  AE 
Sbjct: 3   YISMSVVAFLVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKGTLDPALEAAEA 62

Query: 64  DATMVKVRADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGS 123
              ++ V   G  NFKT+ EAI S+P  NK RV+I +  GVY EK+ I+I +PFIT  G 
Sbjct: 63  ARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFITLLGQ 122

Query: 124 PNDMPTLTFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPNKEGGGPALAARIMG 183
           P     LT+ G +  +GTV+S TL V ++YF AA++ +KN++P P     G ALA RI  
Sbjct: 123 PGAETVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPKPGSQGQALAMRINA 182

Query: 184 NKAAIYNCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG-SV 243
           +KAA Y+C+F+GFQDTLCDD G H +KDC+I+GT DFIFGRG SLYLN QL  V +G  V
Sbjct: 183 DKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAVGDGLRV 242

Query: 244 IAAHSRELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHD 303
           I A  R+   + +GY+FVHC +TG  T  IYLGR+W    +VVY++T+M   V+P GW +
Sbjct: 243 ITAQGRQSATEQNGYTFVHCKVTGTGT-GIYLGRSWMSHPKVVYAFTEMTSVVNPSGWRE 302

Query: 304 MGNPKYTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPP 357
             N  Y  ++ +GEYKC GPGS  ++RV Y++ +   E  P+L+L YI+ + WLLPPP
Sbjct: 303 NLNRGYDKTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWLLPPP 359

[Close]

BLAST of Sed0022128 vs. TAIR 10
Match: AT5G07420.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 334.0 bits (855), Expect = 1.5e-91
Identity = 164/359 (45.68%), Postives = 233/359 (64.90%), Query Frame = 0


Query: 4   YFVTRLAIFLVILASNLASS---SPVPVDKSQLNKWFSDSIKPLDART-ELDKAVVEAEK 63
           Y    L   LV  AS +  +   +P+P D++Q+ +WF  ++KP   R   LD  +  AE 
Sbjct: 3   YISLALVALLVFFASPVVLADDITPIPADRAQIPQWFMANVKPFSQRRGTLDPELEAAEA 62

Query: 64  DATMVKVRADGSGNFKTITEAINSVPANNKKRVLIWIGGGVYKEKLKIEINKPFITFYGS 123
              ++ V  +G G+FKTI  AI S+P  NK RV+I +  G+Y EK+ +++ +P++T  G 
Sbjct: 63  SRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYVTLLGK 122

Query: 124 PNDMPTLTFDGDSKTFGTVDSGTLTVESDYFVAANIILKNSSPRPNKEGGGPALAARIMG 183
           P     LT+ G +  +GTV+S TL V +  F+AAN+ + N+SP P     G ALA RI G
Sbjct: 123 PGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPKPGTQGQALAMRING 182

Query: 184 NKAAIYNCKFYGFQDTLCDDAGMHLYKDCFIQGTTDFIFGRGTSLYLNCQLDVVKEG-SV 243
           +KAA YNC+FYGFQDTLCDD G H +K+C+I+GT DFIFGRG SLYL  QL  V +G  V
Sbjct: 183 DKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAVGDGLRV 242

Query: 244 IAAHSRELEHDGSGYSFVHCTITGPDTKRIYLGRAWRPRSRVVYSYTKMADNVHPEGWHD 303
           IAAH+R+   + +GYSFVHC +TG  T  IYLGRAW    +VVYSYT+M+  V+P GW +
Sbjct: 243 IAAHNRQSTTEQNGYSFVHCKVTGVGT-GIYLGRAWMSHPKVVYSYTEMSSVVNPSGWQE 302

Query: 304 MGNPKYTSSILFGEYKCSGPGSQTDQRVSYSKRLSDAEAKPYLSLNYIEAAKWLLPPPS 358
                +  ++ +GEY C+GPGS   +RV++++ + + EA  +L+L YI+ +KWLLPPP+
Sbjct: 303 NRVRAHDKTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWLLPPPA 360

The following BLAST results are available for this feature:

BLAST of Sed0022128 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152989.1	1.2e-138	68.52	putative pectinesterase 63 [Momordica charantia]	[more]
XP_038902684.1	2.3e-137	67.13	putative pectinesterase 63 [Benincasa hispida]	[more]
XP_022944181.1	2.1e-135	66.01	pectinesterase PPME1-like isoform X3 [Cucurbita moschata]	[more]
KAG6570448.1	1.8e-134	67.54	Pectinesterase PPME1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022944179.1	2.4e-134	67.54	pectinesterase PPME1-like isoform X1 [Cucurbita moschata]	[more]

BLAST of Sed0022128 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LY19	1.8e-100	50.69	Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2	[more]
Q84WM7	1.8e-100	52.52	Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1	[more]
Q9FKF3	1.4e-97	50.14	Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2	[more]
B2VPR8	6.1e-93	49.15	Pectinesterase 2 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
D8VPP5	2.3e-92	48.58	Pectinesterase 1 OS=Olea europaea OX=4146 PE=1 SV=1	[more]

BLAST of Sed0022128 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DHP7	5.8e-139	68.52	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111020595 PE=4 SV=1	[more]
A0A6J1FTQ2	1.0e-135	66.01	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111448705 PE=4 SV=1	[more]
A0A6J1FW87	1.1e-134	67.54	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111448705 PE=4 SV=1	[more]
A0A6J1J8V7	1.3e-133	64.89	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483650 PE=4 SV=1	[more]
A0A5A7V7V3	3.7e-133	64.89	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00480...	[more]

BLAST of Sed0022128 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G69940.1	1.3e-101	52.52	Pectin lyase-like superfamily protein	[more]
AT5G07410.1	1.3e-101	50.69	Pectin lyase-like superfamily protein	[more]
AT5G61680.1	9.9e-99	50.14	Pectin lyase-like superfamily protein	[more]
AT5G07430.1	4.8e-93	46.09	Pectin lyase-like superfamily protein	[more]
AT5G07420.1	1.5e-91	45.68	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 64..347 e-value: 8.6E-58 score: 195.4
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 58..354 e-value: 2.2E-96 score: 325.0
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 30..353
None	No IPR available	PANTHER	PTHR31321:SF87	PECTINESTERASE 63-RELATED	coord: 30..353
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 210..219
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 62..352

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0022128.1	Sed0022128.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity