Sed0021675 (gene) Chayote v1

Overview
NameSed0021675
Typegene
OrganismSechium edule (Chayote v1)
DescriptionCytochrome P450, putative
LocationLG06: 38761225 .. 38766345 (+)
RNA-Seq ExpressionSed0021675
SyntenySed0021675
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTATTCCAATGTTTCTCACAACTCTTTTAGTGATTTTTCTTTCATCTTTAGCAAAGATTGCATATGAGACTGTTACATGCTACTGGCTCACTCCAAGAAGAATCAGAAAGAGGATGGCTGAGCAAGGCGTGCACGGCCCCGACCCGAGACCGCTGATCGGAAACATCGGCGATGTAGCCACGCTCGTGGCGAAATCTACTGCCGACGACATGAGCTCGATCAATCACGACGTCGTCGACCGCTTGATGCCTCATTACACCACTTGGACAAAGCTATACGGTGAGACTATTGCTCCGCCGGACATGGTTATGAATATAAATAAATGATTAAATATTTATATATCATCCACTTAAAAGCGTTCTATTCTAGGTACAAACAATTTTACTTTTTCATATTAAATAATATAATATTTCATCTTGCAATGAATTATTAATGAAAAAAATAACCCACCTTAAAATTTCATTTATCTTTTATTAATTTATTTTGTTGAGTACATATTAGGGGCAAAAGAAAACTAGTTTAGAACCAAACAATAAAACATATTAAGAATGGACTTTTTAAAACATTTACAGGAAACTTAAGAGTTTATGCCGAACTTTTTGTTAAGAATATGAATAAATTATTAAATACTTGATGACACCTTGGTGTTTGGTCGCTTAGTTTCATTGTTTGATTCAAAAAAATAGTATTTGACTTTCATTTATTTGAAAGCAGTATTGCACTCTACGTGAGATCTGTGGTTCTACTGTGCCAATCAATACTAAAATAACTATAAACTCGATAATGCGTAAGCAGGAAAACGATTTATTTTTTGGAACGGGGCAGAGCCAAGGCTGTGTTTGGCGGAGACCGAGTTGATAAAAGAAGTAATGGGGAAAACGAGCCGGGTGTGTGGGAGATCATGGCTGCAAAGACAAGGGACCAAACACTTCATAGGGAAAGGATTGTTGATGGCTAATGGTGAAGAATGGCAACATCAACGTCACATCGTTGCACCTGCGTTTATGGGGGAGAAGCTAAAGGTAACATTTCTTCCCCATCTTTATTCTAAATTTGGTTCATTTTGTTGTATTTTCTTTTTATTTTACTTTCTAAAAGACAATCTTTATATGGACAATGGTTTCATAATTATTAGTTGTTTGACAAATATATAGCAATTTGTTCCGGTACTTTTTAAATAGAGTATTCTTTTTTTTTTTTTTACTGGTTTTATGTTTTCTTTAATTTTAAATTCATAGAATTGAAGTTTTTTTTTTAAAAAATAGAATTGAAATTCTTACTTAAAAATATATAATCGTTTTTTGATGAAGAATAATATTTAAATTTATTAGATTATTTAACAATAATTTAACGAAAAGTGTCTTATGAATTCAAATCTTTCTTTGGTAGAACTAAAAAAAATAGATTATACTACTCTTTTTATTTATTTATGGGATTGTTTTTTTGAAAATATTATTTATGGGATTTTATAAGCTAAATATGGTTAAAAAAAATAAATTTTGTTATATTTTATATATATCCCACACGAATAAAATAAAATATAATAAGTGTCCAAACTTTTTTTCCTTTTTTTCAAAATCTGATTCAATTTTAGATTTGGTAAGAAAATAAACATCTTTCCAAATGGTTTATTTTTTCAAAAACGGAAATCGACCTAAACTTTACTGGACATATATATATATATATATATATATATATAAAACCTTGCCTTAATTTATTTTATTGCCAAAACAATTAGTAGAAAAATAATTCTTAAATAGACTATAAATTGAAAATATTCTTGTTAATTTATTTTCTACTAACTTAATTCTTACCACTAAATCAATTTAGAGTCTTTGACACAGACTGCCACTAAATCGATTGCATAAACAAGAACGTAACATTGGTATTGCATAAATAAGATTTTTTTTTGAATTAGTTTACTAAAAAAAGTAGACCAAAAATGTATGAAATTGTTAGAAGAAGATAGGAAGGGAGAGTTGAATTGACAAAGTTCAAATCAAAATTCTTCCGTTGTACCTTCGGTCTGAAAAAATGAGAAAACATAGGGCCCATATGTACAGAGACAGACAGCATCTTGGAAAAAACATATGTTATTTTCATGTTTGTAATTATATAATTGGAATTTGTATATGGTGTGTGTTTTGTAATGGCATGAACAGAGCTATACGGGGTTCATGGTGGAGTGCACTGAGAAAATGATTCAATCATTGGAGACCGAGTTGGACTCGGGCCGGACCGAGTTTGAAATCGCCGAGTACATGAAGCAACTCACTGCCGACATCATTTCTCGGACCGAGTTCGATACGAGCGCAGACAAAGGCAAGCAAATCTTCCATTTGCTCACTCTTCTTCAGCGCCTCTGCGCCCAAGCCAGCCGCCACCTCTGCCTTCCTGGTAGCCGGTATGCTTAACCATTCTTCTATTCTTTTTATTTTTCAAAAAAAAAAAACACACAAAGTTCAGTTAAAACTGTTTATTTCTAATAGAAATCGAAAACATTTTCGTTCATATATTTTAACTGGAAAATGTTATCGGTTTGTATAAATTCAGAACTGAATTTTTTTTTAGTACATATCAGATAGAAGGAAAATAAATACCGATTCTCGTGAATCAAACCTGTAAGGCTGTGTTGAACTGAATTTTAAAGCCTCAAAAATTCAGAACTGAATTCTAAAGCATAACAGTTCTTGATACGTCACTTGGATTTTAAAAATATAAAACAAAACCAATAAATTAAGAGGTAGAAATAATATTTATATAAGTGAAAAATCAAATAATTTTTGTTTTTTTAAATAACAACATTTGATACTTGAACTCTTAACCATCTTATTATTTGATATATGATGCTTTTTTTTTTCCTTTTTTGCCAAAAATCAAATAGTTATCGAAAGTGATTTATGAATTTAAGATTGTGGTCATAAAAAAAGAATTGAGTTTTTTTTTTTTCACGGTTTCCCTTATATTTTTCTCAATGAAAAGATTATATATATCGTAAAATTTTGTCCTATTAAATTTAAAACTTTATTATGACATATTAATTTCCTTTTCTTTTCTGTTAAAAAAAAAATTTCTTTTTTTTTGAAACGTATAACATATTAATTTCAATAGTATTATTTAACGATCATTCCATTCACGTATATTTTTTGTCAACGGAAAATTTATGTTATTAAAGATTTTATTTTTAAGTCATGATATATTTTTTTTGTGCCTCTGTCGTGATAAAATCTTCTCGGTGGAACAAATATTAATATATAGGGACAAAAGTTAACCTTCTTTACACTATCATAAGTGGGAGAATTCTTTAGAATATTATGATAAGTGGGAGATAAATATTAGGGAAAATTTTAAAATTTTAGTTTTCGTTAATAACTATTTTGATTTTAGATTTTTTCTATATACAGTAATTCTTTAATCACTTAAAAAATATTAATTTTTTTTTATCGAATAAAAAAAAACAATTTTTAAAATTTACTAATTTTTTTCTTAGATTCTAAAATATGAATTGATTTTGAAACATGAATAAAGGGTAGATCATAAAAATTTCCACAAACTAAAAATTATGGCCATTTGGTAAACATTTTATTTTTAGTTTTTTTAAAAAAAATTTAAATCTACAACAATTATCTTACCATAGGTTTTTTTTTTACTTTGATATCTACATCTTATCTATATATTTTAAGAAACCAAACCAAAGTTTGGGAAGAAAAAAAAATAGATTTCAAAAGTTTGTTTTTGTTTTTAAAATTTGGTTAGCAATTCATGTACTTTTTCAAGGAAAGATGTATACACAGTAAGAAAGATGGATCACATTGTAGAAAACTTAAGTAAAAATAAACTCAAATTTCAAAAACCAAAAAAAAAAAAAAAAACCTAAACGTTTACCAAACTGCTTCTAATAAATGTCATTATTTCAGCTGTCATTAATGCATACCCAACCGAAAGTCATAGATTCGAATTTCTCACCCTCAATTATTGTGCTCTAAAAACAATGCCTTAAATATTTTATACTGCATGGTTGCAGTTTTCAGTTTTTTGATGATGTAAAGGTTGACCATATTGCAGGTTTTATCCAAGTAAATACAACAGAGAAATAAAGTCACTAAAAGTAGAAGTGGAAAGGCTGTTAATGGAGATAATTCAAAGTAGAAAAGATGGAGTAGAAATAGGAAGAAGCAGTGGGTATGGAAGTGATTTGTTGGGAATGCTTTTGAAGGAGATGCAAAGAAAAAGAGAGGATGGATTCAGCTTGAACTTGCAGCTCATAATGGATGAATGCAAGACTTTCTTCTTTGCTGGCCATGAAACCACTGCTCTTTTGCTCACTTGGACCATCATGTTGCTCGCCACGAACCCGTCGTGGCAACAAAAGGTTCGAGATGAAGTCAAGCAAGTTTGCAATTCTGGATCAACTCCCTTAATTGAACATCTATCTAAGCTCACTTTGGTAAGCCCATCTACAACTTCTTACAATATGTTTTTTTTTTAAACGAAAAGGTTTTAAGATTTTAGGGTTTAGTGTTCTAACATATCCTAATATTAATTTTAAGTTTTTTTTTCACCAATCGTTTCTGAAGTATTTTGAAAGTTACTCTAAGCAAACCTTTACTTGATATGGTTTTCTCATATTTCCTATATGAATCATCTTCAACAATGTTCAAGCGTATATTTTTCATTAACTTGATATGGTTTTGCAGCTAAATATGGTGATAAATGAATCCCTAAGGCTTTACCCGCCCGCCACAGTTCTTCCAAGAATGGCATTTGAAGACATAAAGATTGGAGATTTAGAGATCCCGAAAGGACTATCGATATGGATCCCAGTACTCGCAATTCATCACAGCCAAGAGTTGTGGGGAAAAGACGCAAACGAGTTCAAGCCCGAAAGATTCGCAGCGAAACCCTTCGTTGCTGGACGTTTCATTCCATTTGCAGCTGGACCAAGAAACTGCATTGGGCAAGGGTTTGCATTGATGGAAGCTAAGATCATCTTGGCTATGTTACTCTCAAAGTTTAGCTTCACTATTTCGGATACTTATCGCCATGCTCCTGTTACTGTTCTCACCATACAGCCTAAGTATGGAGTTCAAGTTTGCTTATCACCATTGAATTCTTGA

mRNA sequence

ATGGCTATTCCAATGTTTCTCACAACTCTTTTAGTGATTTTTCTTTCATCTTTAGCAAAGATTGCATATGAGACTGTTACATGCTACTGGCTCACTCCAAGAAGAATCAGAAAGAGGATGGCTGAGCAAGGCGTGCACGGCCCCGACCCGAGACCGCTGATCGGAAACATCGGCGATGTAGCCACGCTCGTGGCGAAATCTACTGCCGACGACATGAGCTCGATCAATCACGACGTCGTCGACCGCTTGATGCCTCATTACACCACTTGGACAAAGCTATACGGAAAACGATTTATTTTTTGGAACGGGGCAGAGCCAAGGCTGTGTTTGGCGGAGACCGAGTTGATAAAAGAAGTAATGGGGAAAACGAGCCGGGTGTGTGGGAGATCATGGCTGCAAAGACAAGGGACCAAACACTTCATAGGGAAAGGATTGTTGATGGCTAATGGTGAAGAATGGCAACATCAACGTCACATCGTTGCACCTGCGTTTATGGGGGAGAAGCTAAAGAGCTATACGGGGTTCATGGTGGAGTGCACTGAGAAAATGATTCAATCATTGGAGACCGAGTTGGACTCGGGCCGGACCGAGTTTGAAATCGCCGAGTACATGAAGCAACTCACTGCCGACATCATTTCTCGGACCGAGTTCGATACGAGCGCAGACAAAGGCAAGCAAATCTTCCATTTGCTCACTCTTCTTCAGCGCCTCTGCGCCCAAGCCAGCCGCCACCTCTGCCTTCCTGGTAGCCGGTTTTATCCAAGTAAATACAACAGAGAAATAAAGTCACTAAAAGTAGAAGTGGAAAGGCTGTTAATGGAGATAATTCAAAGTAGAAAAGATGGAGTAGAAATAGGAAGAAGCAGTGGGTATGGAAGTGATTTGTTGGGAATGCTTTTGAAGGAGATGCAAAGAAAAAGAGAGGATGGATTCAGCTTGAACTTGCAGCTCATAATGGATGAATGCAAGACTTTCTTCTTTGCTGGCCATGAAACCACTGCTCTTTTGCTCACTTGGACCATCATGTTGCTCGCCACGAACCCGTCGTGGCAACAAAAGGTTCGAGATGAAGTCAAGCAAGTTTGCAATTCTGGATCAACTCCCTTAATTGAACATCTATCTAAGCTCACTTTGCTAAATATGGTGATAAATGAATCCCTAAGGCTTTACCCGCCCGCCACAGTTCTTCCAAGAATGGCATTTGAAGACATAAAGATTGGAGATTTAGAGATCCCGAAAGGACTATCGATATGGATCCCAGTACTCGCAATTCATCACAGCCAAGAGTTGTGGGGAAAAGACGCAAACGAGTTCAAGCCCGAAAGATTCGCAGCGAAACCCTTCGTTGCTGGACGTTTCATTCCATTTGCAGCTGGACCAAGAAACTGCATTGGGCAAGGGTTTGCATTGATGGAAGCTAAGATCATCTTGGCTATGTTACTCTCAAAGTTTAGCTTCACTATTTCGGATACTTATCGCCATGCTCCTGTTACTGTTCTCACCATACAGCCTAAGTATGGAGTTCAAGTTTGCTTATCACCATTGAATTCTTGA

Coding sequence (CDS)

ATGGCTATTCCAATGTTTCTCACAACTCTTTTAGTGATTTTTCTTTCATCTTTAGCAAAGATTGCATATGAGACTGTTACATGCTACTGGCTCACTCCAAGAAGAATCAGAAAGAGGATGGCTGAGCAAGGCGTGCACGGCCCCGACCCGAGACCGCTGATCGGAAACATCGGCGATGTAGCCACGCTCGTGGCGAAATCTACTGCCGACGACATGAGCTCGATCAATCACGACGTCGTCGACCGCTTGATGCCTCATTACACCACTTGGACAAAGCTATACGGAAAACGATTTATTTTTTGGAACGGGGCAGAGCCAAGGCTGTGTTTGGCGGAGACCGAGTTGATAAAAGAAGTAATGGGGAAAACGAGCCGGGTGTGTGGGAGATCATGGCTGCAAAGACAAGGGACCAAACACTTCATAGGGAAAGGATTGTTGATGGCTAATGGTGAAGAATGGCAACATCAACGTCACATCGTTGCACCTGCGTTTATGGGGGAGAAGCTAAAGAGCTATACGGGGTTCATGGTGGAGTGCACTGAGAAAATGATTCAATCATTGGAGACCGAGTTGGACTCGGGCCGGACCGAGTTTGAAATCGCCGAGTACATGAAGCAACTCACTGCCGACATCATTTCTCGGACCGAGTTCGATACGAGCGCAGACAAAGGCAAGCAAATCTTCCATTTGCTCACTCTTCTTCAGCGCCTCTGCGCCCAAGCCAGCCGCCACCTCTGCCTTCCTGGTAGCCGGTTTTATCCAAGTAAATACAACAGAGAAATAAAGTCACTAAAAGTAGAAGTGGAAAGGCTGTTAATGGAGATAATTCAAAGTAGAAAAGATGGAGTAGAAATAGGAAGAAGCAGTGGGTATGGAAGTGATTTGTTGGGAATGCTTTTGAAGGAGATGCAAAGAAAAAGAGAGGATGGATTCAGCTTGAACTTGCAGCTCATAATGGATGAATGCAAGACTTTCTTCTTTGCTGGCCATGAAACCACTGCTCTTTTGCTCACTTGGACCATCATGTTGCTCGCCACGAACCCGTCGTGGCAACAAAAGGTTCGAGATGAAGTCAAGCAAGTTTGCAATTCTGGATCAACTCCCTTAATTGAACATCTATCTAAGCTCACTTTGCTAAATATGGTGATAAATGAATCCCTAAGGCTTTACCCGCCCGCCACAGTTCTTCCAAGAATGGCATTTGAAGACATAAAGATTGGAGATTTAGAGATCCCGAAAGGACTATCGATATGGATCCCAGTACTCGCAATTCATCACAGCCAAGAGTTGTGGGGAAAAGACGCAAACGAGTTCAAGCCCGAAAGATTCGCAGCGAAACCCTTCGTTGCTGGACGTTTCATTCCATTTGCAGCTGGACCAAGAAACTGCATTGGGCAAGGGTTTGCATTGATGGAAGCTAAGATCATCTTGGCTATGTTACTCTCAAAGTTTAGCTTCACTATTTCGGATACTTATCGCCATGCTCCTGTTACTGTTCTCACCATACAGCCTAAGTATGGAGTTCAAGTTTGCTTATCACCATTGAATTCTTGA

Protein sequence

MAIPMFLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDVATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVMGKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECTEKMIQSLETELDSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLLKEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDEVKQVCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSIWIPVLAIHHSQELWGKDANEFKPERFAAKPFVAGRFIPFAAGPRNCIGQGFALMEAKIILAMLLSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLNS
Homology
BLAST of Sed0021675 vs. NCBI nr
Match: XP_038905687.1 (cytokinin hydroxylase-like [Benincasa hispida])

HSP 1 Score: 923.7 bits (2386), Expect = 7.0e-265
Identity = 448/518 (86.49%), Postives = 488/518 (94.21%), Query Frame = 0

Query: 1   MAIPMFLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDV 60
           MAI +FLTT+LVIF+SSLAKIAY+T++CYWLTPRRIRK+M +QGV GPDPRPL+GNI DV
Sbjct: 1   MAIVIFLTTILVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVCGPDPRPLLGNILDV 60

Query: 61  ATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVM 120
           A LVAKSTA+DM SI+H++V RL+PHYTTWTK YGKRFI+WNG EPRLCLAETELIKEV+
Sbjct: 61  AALVAKSTANDMKSIDHNIVGRLLPHYTTWTKQYGKRFIYWNGTEPRLCLAETELIKEVL 120

Query: 121 GKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECT 180
            K S VCG+SWLQRQGTKHFIGKGLLMANGE+W HQRHIVAPAFMG+KLKSY G MVECT
Sbjct: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGLMVECT 180

Query: 181 EKMIQSLETELDSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQ 240
           +KMIQSLETE DSGRTEFEIA+YMKQLTADIISRTEFD ++DKGK+IFHLLTLLQRLCAQ
Sbjct: 181 KKMIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQ 240

Query: 241 ASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLL 300
           ASRHLCLPGSRF+PSKYNREIKSLK+EVERLLMEIIQSRKDGVEIGRS+ YGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 KEMQRKRED-GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDEVK 360
            EMQRKRED GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNP WQ KVR EV 
Sbjct: 301 NEMQRKREDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPDWQHKVRHEVN 360

Query: 361 QVCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSIWI 420
           QVCN+G +P +EHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSIWI
Sbjct: 361 QVCNNGGSPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWI 420

Query: 421 PVLAIHHSQELWGKDANEFKPERFAAKPFVAGRFIPFAAGPRNCIGQGFALMEAKIILAM 480
           PVLAIHHS+ELWG+DANEFKPERFA+KPF  GRFIPFA+GPRNCIGQGFALMEAKIILAM
Sbjct: 421 PVLAIHHSEELWGEDANEFKPERFASKPFSGGRFIPFASGPRNCIGQGFALMEAKIILAM 480

Query: 481 LLSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLNS 518
            +SKFSFTISD+YRHAPV+VLTIQPKYGVQV L+P+NS
Sbjct: 481 FISKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 518

BLAST of Sed0021675 vs. NCBI nr
Match: XP_008461587.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])

HSP 1 Score: 922.9 bits (2384), Expect = 1.2e-264
Identity = 448/520 (86.15%), Postives = 489/520 (94.04%), Query Frame = 0

Query: 1   MAIPMFLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDV 60
           MAI +FLTTLLVIF+SSLAKIAY+T++CYWLTPRRIRK+M +QGV GP PRPL+GNI DV
Sbjct: 1   MAIIIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  ATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVM 120
           A L+AKSTA DMSSI+H++VDRL+PHYTTWTK YGKRFI+WNG EPRLCLAETELIKEV+
Sbjct: 61  AALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 GKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECT 180
            K S VCGRSWLQRQGTKHFIGKGLLMANGE+W HQRHIVAP+FMG+KLKSY G MVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 EKMIQSLETELDSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQ 240
           +K+IQSLETELDSGRTEFEIA+YMKQLTADIISRTEFD ++DKG QIFHLLTLLQ LCAQ
Sbjct: 181 DKLIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLL 300
           ASRHLCLPGSRF+PSKYNREIKSLK+EVERLLMEIIQSRKDGVEIGRS+ YGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 KEMQRKRED---GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDE 360
            EMQRK++D   GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNP+WQQKVR E
Sbjct: 301 NEMQRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VKQVCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSI 420
           V QVC++G+TP +EHLSK TLLNMVINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSI
Sbjct: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420

Query: 421 WIPVLAIHHSQELWGKDANEFKPERFAAKPFVAGRFIPFAAGPRNCIGQGFALMEAKIIL 480
           WIPVLAIHHS+ELWGKD NEFKPERFA+KPF  GRFIPFAAGPRNC+GQGFALMEAKIIL
Sbjct: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKIIL 480

Query: 481 AMLLSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLNS 518
           AMLLSKFSFTISD+YRHAPV+VLTIQPKYGVQV L+P+NS
Sbjct: 481 AMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Sed0021675 vs. NCBI nr
Match: XP_004145660.1 (cytokinin hydroxylase [Cucumis sativus] >KGN57750.1 hypothetical protein Csa_009800 [Cucumis sativus])

HSP 1 Score: 915.6 bits (2365), Expect = 1.9e-262
Identity = 445/520 (85.58%), Postives = 485/520 (93.27%), Query Frame = 0

Query: 1   MAIPMFLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDV 60
           MAI + LTTLLVIF+SSLAKIAY+T++CYWLTPRRIRK+M +QGV GP PRPL+GNI DV
Sbjct: 1   MAIMIILTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  ATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVM 120
           A L+ KSTA DMSSI+H++VDRL+PHYTTWTK YGKRFI+WNG EPRLCLAETELIKEV+
Sbjct: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 GKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECT 180
            K S VCGRSWLQRQGTKHFIGKGLLMANGE+W HQRHIVAP+FMG+KLKSY G MVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 EKMIQSLETELDSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQ 240
            K+IQSLETE DSGRTEFEIA+YMKQLTADIISRTEFD ++DKG QIFHLLTLLQ LCAQ
Sbjct: 181 NKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDCNSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLL 300
           ASRHLCLPGSRF+PSKYNREIKSLK+EVERLLMEIIQSRKDGVEIGRS+ YGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 KEMQRKRED---GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDE 360
            EMQ++RED   GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNP+WQQKVR E
Sbjct: 301 NEMQKRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VKQVCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSI 420
           V QVC++G+TP +EHLSK TLLNMVINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSI
Sbjct: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420

Query: 421 WIPVLAIHHSQELWGKDANEFKPERFAAKPFVAGRFIPFAAGPRNCIGQGFALMEAKIIL 480
           WIPVLAIHHS+ELWGKD NEFKPERFA+KPF  GRFIPFAAGPRNCIGQGFALMEAKIIL
Sbjct: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIIL 480

Query: 481 AMLLSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLNS 518
           AMLLSKFSF+ISD+YRHAPV+VLTIQPKYGVQV L+P+NS
Sbjct: 481 AMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Sed0021675 vs. NCBI nr
Match: XP_022983651.1 (cytokinin hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 871.7 bits (2251), Expect = 3.2e-249
Identity = 433/518 (83.59%), Postives = 467/518 (90.15%), Query Frame = 0

Query: 1   MAIPMFLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDV 60
           MA  +FLTTL VIF+SSLAKI+Y+T + Y LTPRRIRK M +QGV GP PR LIGNI DV
Sbjct: 1   MAFIIFLTTLFVIFISSLAKISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60

Query: 61  ATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVM 120
             LVAKSTA+DMSSI+HD+VDRL+PHYT WTK YGKRFI+WNG EPRLCLAETELIKE++
Sbjct: 61  VGLVAKSTANDMSSIDHDIVDRLLPHYTIWTKQYGKRFIYWNGTEPRLCLAETELIKELL 120

Query: 121 GKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECT 180
           GK S VCGRSWLQRQGTKHFIGKGLLMANGE+W HQRHIVAPAFMG+KLKSY GFMVECT
Sbjct: 121 GKKSSVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGFMVECT 180

Query: 181 EKMIQSLETELDSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQ 240
            KMIQS+ETELDSGRTEFEIA+YMKQLTADIISRTEFDT +DKG QIFHLLTLLQRLCAQ
Sbjct: 181 NKMIQSMETELDSGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQ 240

Query: 241 ASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLL 300
           ASRHLCLPGSRF PSKYNREIKSLK EVERLLMEII+SRKDGVEIGRSS YGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLL 300

Query: 301 KEM-QRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDEVK 360
            EM QRK     +LNLQLI+DECKTFFFAGHETTALLLTWTIMLLATNP+WQ  VR EV 
Sbjct: 301 NEMQQRKNSTTTTLNLQLIIDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAEVN 360

Query: 361 QVCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSIWI 420
           QVCN+  TP I+HLSKLTLLNMVINESLRLYPPATVLPRMA ED+K+GD++IPKGLSIWI
Sbjct: 361 QVCNA-RTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWI 420

Query: 421 PVLAIHHSQELWGKDANEFKPERFAAKPFVAGRFIPFAAGPRNCIGQGFALMEAKIILAM 480
           PVLAIHHS+ELWGKDAN+F P RFAAK F  GRFIPFA GPRNCIGQGFA+MEAKIILAM
Sbjct: 421 PVLAIHHSEELWGKDANQFNPNRFAAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAM 480

Query: 481 LLSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLNS 518
            LSKFSFTISDTYRHAPV VLTIQPK+GVQVCL+P +S
Sbjct: 481 FLSKFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPFSS 517

BLAST of Sed0021675 vs. NCBI nr
Match: XP_023528555.1 (cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 866.7 bits (2238), Expect = 1.0e-247
Identity = 428/521 (82.15%), Postives = 468/521 (89.83%), Query Frame = 0

Query: 1   MAIPMFLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDV 60
           MA  +FLTTLL+IF++SLA+I+Y+T + Y LTPRRIRK M +QGV GP PR LIGNI DV
Sbjct: 1   MAFIIFLTTLLLIFIASLARISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60

Query: 61  ATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVM 120
           A LVAKSTA+DMSSI+HD+VDRL+PHYT WTK YGKRFI+WNG EPRLCLAETELIKE++
Sbjct: 61  AGLVAKSTANDMSSIDHDIVDRLLPHYTIWTKQYGKRFIYWNGTEPRLCLAETELIKELL 120

Query: 121 GKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECT 180
           GK S VCGRSWLQRQGTKHFIGKGLLMANGE+W HQRHIVAPAFMG+KLKSY GFMVECT
Sbjct: 121 GKKSSVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGFMVECT 180

Query: 181 EKMIQSLETELDSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQ 240
            KMIQS+ETEL+SGRTEFEIA+YMKQLTADIISRTEFDT +DKG QIFHLLTLLQRLCAQ
Sbjct: 181 NKMIQSMETELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQ 240

Query: 241 ASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLL 300
           ASRHLCLPGSRF PSKYNREIKSLK EVERLLMEII+SRKDGVEIGRSS YGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLL 300

Query: 301 KEMQRKRED----GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRD 360
            E+Q+++        +LNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNP+WQ  VR 
Sbjct: 301 NELQQRKNSSTTTSTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRA 360

Query: 361 EVKQVCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLS 420
           +V QVCN+  TP I+HLSKLTLLNMVINESLRLYPPATVLPRMA ED+K+GD++IPKGLS
Sbjct: 361 QVNQVCNA-RTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLS 420

Query: 421 IWIPVLAIHHSQELWGKDANEFKPERFAAKPFVAGRFIPFAAGPRNCIGQGFALMEAKII 480
           IWIPVLAIHHS+ELWGKDAN+F P RF AK F  GRFIPFA GPRNCIGQGFA+MEAKII
Sbjct: 421 IWIPVLAIHHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKII 480

Query: 481 LAMLLSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLNS 518
           LAMLLSKF FTISDTYRHAPV VLTIQPKYGVQVCL+P  S
Sbjct: 481 LAMLLSKFRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 520

BLAST of Sed0021675 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 9.7e-209
Identity = 350/513 (68.23%), Postives = 423/513 (82.46%), Query Frame = 0

Query: 7   LTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDVATLVAK 66
           L +LLVIF++++ ++ Y+T++CYWLTPRRI+K M +QGV GP PRPL GNI +++ +V++
Sbjct: 6   LKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISAMVSQ 65

Query: 67  STADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVMGKTSRV 126
           S + D  SI+HD+V RL+PHY  W+K YGKRFI WNG +PRLCL ETELIKE++ K + V
Sbjct: 66  SASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMKHNGV 125

Query: 127 CGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECTEKMIQS 186
            GRSWLQ+QGTK+FIG+GLLMANG++W HQRH+ APAF GE+LK Y   MVECT K+++ 
Sbjct: 126 SGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVER 185

Query: 187 LETELDSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQASRHLC 246
           L  E+  G  E EI E M +LTADIISRT+F +S +KGK++F+ LT+LQR CAQA+RHLC
Sbjct: 186 LRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLC 245

Query: 247 LPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLLKEMQ-R 306
            PGSRF PSKYNREIKSLK EVERLL+EIIQSR+D  E+GRSS +G DLLG+LL EM   
Sbjct: 246 FPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDID 305

Query: 307 KREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDEVKQVCNSG 366
           K  +  + NLQLIMDECKTFFFAGHETTALLLTWT MLLA NP+WQ+KVR+EV++V    
Sbjct: 306 KNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRN 365

Query: 367 STPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSIWIPVLAIH 426
             P ++ LSKLT L+ VINESLRLYPPAT+LPRMAFED+K+GDL IPKGLSIWIPVLAIH
Sbjct: 366 GLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIH 425

Query: 427 HSQELWGKDANEFKPERFAAKPFVAGR-FIPFAAGPRNCIGQGFALMEAKIILAMLLSKF 486
           HS+ELWGKDAN+F PERF  +PF +GR FIPFAAGPRNCIGQ FALMEAKIILA L+SKF
Sbjct: 426 HSEELWGKDANQFNPERFGGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLISKF 485

Query: 487 SFTISDTYRHAPVTVLTIQPKYGVQVCLSPLNS 518
           +FTIS  YRHAP+ VLTI+PKYGVQV L PL S
Sbjct: 486 NFTISKNYRHAPIVVLTIKPKYGVQVILKPLVS 518

BLAST of Sed0021675 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 3.6e-203
Identity = 344/517 (66.54%), Postives = 415/517 (80.27%), Query Frame = 0

Query: 1   MAIPMFLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDV 60
           M + + L  +LVI ++ + ++ Y+++ CY+LTPRRI+K M  QG+ GP PR L GNI D+
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 61  ATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVM 120
           + +++ S ++D SSI+H++V RL+PHY +W+K YGKRFI WNG EPRLCL ETE+IKE++
Sbjct: 61  SKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELL 120

Query: 121 GKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECT 180
            K + V G+SWLQ+QGTK FIG+GLLMANGE W HQRH+ APAF  ++LK Y   MVECT
Sbjct: 121 TKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 180

Query: 181 EKMIQSLETELDSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQ 240
           + M + L  E+     E EI E M++LTADIISRTEF +S DKGK++F LLT+LQRLCAQ
Sbjct: 181 KMMAERLRKEVGE---EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQ 240

Query: 241 ASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLL 300
           A+RHLC PGSRF PSKYNREIKSLK EVERLLMEII SRKD VEIGRSS YG DLLG+LL
Sbjct: 241 ATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLL 300

Query: 301 KEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDEVKQ 360
            +M   +    +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP+WQ  VRDEV+Q
Sbjct: 301 NQMDSNKN---NLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQ 360

Query: 361 VCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSIWIP 420
           VC     P +E LS LT LN VINESLRLYPPAT+LPRMAFEDIK+GDL IPKGLSIWIP
Sbjct: 361 VCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIP 420

Query: 421 VLAIHHSQELWGKDANEFKPERFAAKPFVAGR-FIPFAAGPRNCIGQGFALMEAKIILAM 480
           VLAIHHS ELWG+DANEF PERF  + F + R F+PFAAGPRNCIGQ FA+MEAKIILAM
Sbjct: 421 VLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAM 480

Query: 481 LLSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLN 517
           L+SKFSF IS+ YRHAP+ VLTI+PKYGVQ+ L PL+
Sbjct: 481 LVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Sed0021675 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 4.1e-98
Identity = 196/525 (37.33%), Postives = 306/525 (58.29%), Query Frame = 0

Query: 1   MAIPMFLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDV 60
           M + +FL  +++I +  L +I    +   W  P  + +R+  QG+ GP+ R   GN+ ++
Sbjct: 1   MGLVIFLALIVLILIIGL-RIFKAFMILVW-HPFVLTRRLKNQGISGPNYRIFYGNLSEI 60

Query: 61  ATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVM 120
             +  +S    +   ++D+  R++PHY  W   YG+ F++WNG EPR+C+++ EL K ++
Sbjct: 61  KKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTML 120

Query: 121 GKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECT 180
                   +S  + +  K    KGL+   G +W   R I+ PAF  ++LK  T  MV+CT
Sbjct: 121 SNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCT 180

Query: 181 EKMIQSLETELDSGRTEF-----EIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQ 240
            KM++    E     TE      E+ E  ++LTADII+ + F +S  +G ++F     L+
Sbjct: 181 LKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELK 240

Query: 241 RLCAQASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDL 300
           R    +   + +PG+++ P+  N  +  L+ +++  +  II SR     +   S YG DL
Sbjct: 241 RCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYGDDL 300

Query: 301 LGMLLKEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVR 360
           LG+LLK    + ++   ++++ I+ EC+TFFF GHETT+ LL WT MLL+ +  WQ+K+R
Sbjct: 301 LGILLKAYNTEGKER-KMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLR 360

Query: 361 DEVKQVCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGL 420
           +E+ + C    TP  E  SKL L+NMVI ESLRLY P + L R A  +IK+GDLEIPKG 
Sbjct: 361 EEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPKGT 420

Query: 421 SIWIPVLAIHHSQELWGKDANEFKPERFAAKPFVAGR----FIPFAAGPRNCIGQGFALM 480
           ++ IP+L +H  + LWG DA++F P RFA     A       + F+ GPR CIGQ F ++
Sbjct: 421 TVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNFVMI 480

Query: 481 EAKIILAMLLSKFSF-TISDTYRHAPVTVLTIQPKYGVQVCLSPL 516
           EAK +L M+L +F F ++ D Y+H PV  +TIQP+YG+ V L PL
Sbjct: 481 EAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Sed0021675 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 4.5e-97
Identity = 193/493 (39.15%), Postives = 286/493 (58.01%), Query Frame = 0

Query: 30  WLTPRRIRKRMAEQGVHGPDPRPLIGNIGDVATLVAKSTADDMSSINHDVVDRLMPHYTT 89
           W+  RR +K    QG+ GP  R L GN+ ++  +  ++    +   ++D+V R++PH   
Sbjct: 89  WMLSRRFKK----QGISGPKYRILYGNLREIRKMKNEAKLMVLDPNSNDIVPRVLPHLQQ 148

Query: 90  WTKLYGKRFIFWNGAEPRLCLAETELIKEVMGKTSRVCGRSWLQRQGTKHFIGKGLLMAN 149
           W   YG+ F++W G +PRLC+++ EL K+++        +S  + +  K   G GL+  N
Sbjct: 149 WKSQYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSKSKTKPEILK-LSGNGLIFVN 208

Query: 150 GEEWQHQRHIVAPAFMGEKLKSYTGFMVECTEKMIQSLETELDSGRTE--FEIAEYMKQL 209
           G +W   R I+ PAF  +KLK  T  MV+CT +M    + + +   TE    I+   K+L
Sbjct: 209 GLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKKQRNGVETEQFVLISREFKRL 268

Query: 210 TADIISRTEFDTSADKGKQIFHLLTLLQRLCAQASRHLCLPGSRFYPSKYNREIKSLKVE 269
           TADII+   F +S  +G ++F     LQ+ CA A   L  PG ++ P+  N +I  L ++
Sbjct: 269 TADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSNLQIWKLDMK 328

Query: 270 VERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLLKEMQRKREDGFSLNLQLIMDECKTFFF 329
           V   +  II +R        S  YG+DLLG++L       E    +++  I++ECKTFFF
Sbjct: 329 VNSSIKRIIDARL----TSESKDYGNDLLGIMLTAAS-SNESEKKMSIDEIIEECKTFFF 388

Query: 330 AGHETTALLLTWTIMLLATNPSWQQKVRDEVKQVCNSGSTPLIEHLSKLTLLNMVINESL 389
           AGHETTA LLTW+ MLL+ +  WQ+K+R+EV   C     P  E  SKL L+N V  ESL
Sbjct: 389 AGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAETCSKLKLMNTVFMESL 448

Query: 390 RLYPPATVLPRMAFEDIKIGDLEIPKGLSIWIPVLAIHHSQELWGKDANEFKPERFAAKP 449
           RLY P   L R+A ED+K+G+LEIPKG +I +P+  +H  + +WG DA++F P RFA   
Sbjct: 449 RLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWGSDADKFNPMRFANGL 508

Query: 450 FVAGR----FIPFAAGPRNCIGQGFALMEAKIILAMLLSKFSFTISDTYRHAPVTVLTIQ 509
             A       + F+ GPR CIGQ FA+MEAK +LAM+L +F   +S  Y+HAP   LT+Q
Sbjct: 509 SRAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLNLSADYKHAPADHLTLQ 568

Query: 510 PKYGVQVCLSPLN 517
           P+Y + V L P++
Sbjct: 569 PQYDLPVILEPID 571

BLAST of Sed0021675 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 3.2e-95
Identity = 184/521 (35.32%), Postives = 302/521 (57.97%), Query Frame = 0

Query: 6   FLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDVATLVA 65
           F+  +LV+ +  L+ +  + ++  W  PR+I +  ++QG+ GP     IGN+ ++  ++ 
Sbjct: 9   FIPKVLVLSV-ILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMML 68

Query: 66  KSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVMGKTSR 125
           K+++  M   +H+++ R++  Y  W K+YG  F+ W G   RL +A+ +LI+E+  K+  
Sbjct: 69  KASSHPM-PFSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSEF 128

Query: 126 VCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECTEKMIQ 185
                       K   G GLL   GE+W H R I++P F  E LK     +++    M+ 
Sbjct: 129 YEKNE--AHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVD 188

Query: 186 SLETEL-DSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQASRH 245
               +L ++G  E ++ E+ + LT D+ISRT F +S + G+ +F L      LCA+A + 
Sbjct: 189 KWSDKLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQK 248

Query: 246 LCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRK----DGVEIGRSSGYGSDLLGMLL 305
           + +PG RF+P++ N +   L  E+ + L+++I+ R+    DG           DLLG+++
Sbjct: 249 VFIPGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI 308

Query: 306 KEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDEVKQ 365
           +          ++ +Q I++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ K RDEV +
Sbjct: 309 QAK--------NVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLR 368

Query: 366 VCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSIWIP 425
           VC S   P  +H+ KL  L+M++NESLRLYPP     R A  D+K+G  +IP G  + IP
Sbjct: 369 VCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIP 428

Query: 426 VLAIHHSQELWGKDANEFKPERFA-AKPFVAGR---FIPFAAGPRNCIGQGFALMEAKII 485
           ++A+HH Q +WG D NEF P RFA   P  A     FIPF  G R CIGQ  A+++AK+ 
Sbjct: 429 IIAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLT 488

Query: 486 LAMLLSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLNS 518
           LA+++ +F+F ++ TY+HAP  ++ + P++G  +    L +
Sbjct: 489 LAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLTN 517

BLAST of Sed0021675 vs. ExPASy TrEMBL
Match: A0A1S3CGC0 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=3 SV=1)

HSP 1 Score: 922.9 bits (2384), Expect = 5.8e-265
Identity = 448/520 (86.15%), Postives = 489/520 (94.04%), Query Frame = 0

Query: 1   MAIPMFLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDV 60
           MAI +FLTTLLVIF+SSLAKIAY+T++CYWLTPRRIRK+M +QGV GP PRPL+GNI DV
Sbjct: 1   MAIIIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  ATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVM 120
           A L+AKSTA DMSSI+H++VDRL+PHYTTWTK YGKRFI+WNG EPRLCLAETELIKEV+
Sbjct: 61  AALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 GKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECT 180
            K S VCGRSWLQRQGTKHFIGKGLLMANGE+W HQRHIVAP+FMG+KLKSY G MVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 EKMIQSLETELDSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQ 240
           +K+IQSLETELDSGRTEFEIA+YMKQLTADIISRTEFD ++DKG QIFHLLTLLQ LCAQ
Sbjct: 181 DKLIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLL 300
           ASRHLCLPGSRF+PSKYNREIKSLK+EVERLLMEIIQSRKDGVEIGRS+ YGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 KEMQRKRED---GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDE 360
            EMQRK++D   GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNP+WQQKVR E
Sbjct: 301 NEMQRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VKQVCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSI 420
           V QVC++G+TP +EHLSK TLLNMVINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSI
Sbjct: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420

Query: 421 WIPVLAIHHSQELWGKDANEFKPERFAAKPFVAGRFIPFAAGPRNCIGQGFALMEAKIIL 480
           WIPVLAIHHS+ELWGKD NEFKPERFA+KPF  GRFIPFAAGPRNC+GQGFALMEAKIIL
Sbjct: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKIIL 480

Query: 481 AMLLSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLNS 518
           AMLLSKFSFTISD+YRHAPV+VLTIQPKYGVQV L+P+NS
Sbjct: 481 AMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Sed0021675 vs. ExPASy TrEMBL
Match: A0A0A0L6Y6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=3 SV=1)

HSP 1 Score: 915.6 bits (2365), Expect = 9.2e-263
Identity = 445/520 (85.58%), Postives = 485/520 (93.27%), Query Frame = 0

Query: 1   MAIPMFLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDV 60
           MAI + LTTLLVIF+SSLAKIAY+T++CYWLTPRRIRK+M +QGV GP PRPL+GNI DV
Sbjct: 1   MAIMIILTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  ATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVM 120
           A L+ KSTA DMSSI+H++VDRL+PHYTTWTK YGKRFI+WNG EPRLCLAETELIKEV+
Sbjct: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 GKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECT 180
            K S VCGRSWLQRQGTKHFIGKGLLMANGE+W HQRHIVAP+FMG+KLKSY G MVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 EKMIQSLETELDSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQ 240
            K+IQSLETE DSGRTEFEIA+YMKQLTADIISRTEFD ++DKG QIFHLLTLLQ LCAQ
Sbjct: 181 NKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDCNSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLL 300
           ASRHLCLPGSRF+PSKYNREIKSLK+EVERLLMEIIQSRKDGVEIGRS+ YGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 KEMQRKRED---GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDE 360
            EMQ++RED   GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNP+WQQKVR E
Sbjct: 301 NEMQKRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VKQVCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSI 420
           V QVC++G+TP +EHLSK TLLNMVINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSI
Sbjct: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420

Query: 421 WIPVLAIHHSQELWGKDANEFKPERFAAKPFVAGRFIPFAAGPRNCIGQGFALMEAKIIL 480
           WIPVLAIHHS+ELWGKD NEFKPERFA+KPF  GRFIPFAAGPRNCIGQGFALMEAKIIL
Sbjct: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIIL 480

Query: 481 AMLLSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLNS 518
           AMLLSKFSF+ISD+YRHAPV+VLTIQPKYGVQV L+P+NS
Sbjct: 481 AMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Sed0021675 vs. ExPASy TrEMBL
Match: A0A6J1J2Y1 (cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111482203 PE=3 SV=1)

HSP 1 Score: 871.7 bits (2251), Expect = 1.5e-249
Identity = 433/518 (83.59%), Postives = 467/518 (90.15%), Query Frame = 0

Query: 1   MAIPMFLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDV 60
           MA  +FLTTL VIF+SSLAKI+Y+T + Y LTPRRIRK M +QGV GP PR LIGNI DV
Sbjct: 1   MAFIIFLTTLFVIFISSLAKISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60

Query: 61  ATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVM 120
             LVAKSTA+DMSSI+HD+VDRL+PHYT WTK YGKRFI+WNG EPRLCLAETELIKE++
Sbjct: 61  VGLVAKSTANDMSSIDHDIVDRLLPHYTIWTKQYGKRFIYWNGTEPRLCLAETELIKELL 120

Query: 121 GKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECT 180
           GK S VCGRSWLQRQGTKHFIGKGLLMANGE+W HQRHIVAPAFMG+KLKSY GFMVECT
Sbjct: 121 GKKSSVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGFMVECT 180

Query: 181 EKMIQSLETELDSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQ 240
            KMIQS+ETELDSGRTEFEIA+YMKQLTADIISRTEFDT +DKG QIFHLLTLLQRLCAQ
Sbjct: 181 NKMIQSMETELDSGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQ 240

Query: 241 ASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLL 300
           ASRHLCLPGSRF PSKYNREIKSLK EVERLLMEII+SRKDGVEIGRSS YGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLL 300

Query: 301 KEM-QRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDEVK 360
            EM QRK     +LNLQLI+DECKTFFFAGHETTALLLTWTIMLLATNP+WQ  VR EV 
Sbjct: 301 NEMQQRKNSTTTTLNLQLIIDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAEVN 360

Query: 361 QVCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSIWI 420
           QVCN+  TP I+HLSKLTLLNMVINESLRLYPPATVLPRMA ED+K+GD++IPKGLSIWI
Sbjct: 361 QVCNA-RTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWI 420

Query: 421 PVLAIHHSQELWGKDANEFKPERFAAKPFVAGRFIPFAAGPRNCIGQGFALMEAKIILAM 480
           PVLAIHHS+ELWGKDAN+F P RFAAK F  GRFIPFA GPRNCIGQGFA+MEAKIILAM
Sbjct: 421 PVLAIHHSEELWGKDANQFNPNRFAAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAM 480

Query: 481 LLSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLNS 518
            LSKFSFTISDTYRHAPV VLTIQPK+GVQVCL+P +S
Sbjct: 481 FLSKFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPFSS 517

BLAST of Sed0021675 vs. ExPASy TrEMBL
Match: A0A6J1F8S1 (cytokinin hydroxylase OS=Cucurbita moschata OX=3662 GN=LOC111441866 PE=3 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 8.4e-248
Identity = 426/517 (82.40%), Postives = 465/517 (89.94%), Query Frame = 0

Query: 1   MAIPMFLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDV 60
           MA  +FLTTLL+IFL+SLA+I+Y+T + Y LTPRRIRK M +QGV GP PR LIGNI DV
Sbjct: 1   MAFIIFLTTLLLIFLASLARISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60

Query: 61  ATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVM 120
           A LVAKSTA+ MSSI+HD+VDRL+PHYT WTK YGKRFI+WNG EPRLCLAETELIKE++
Sbjct: 61  AGLVAKSTANHMSSIDHDIVDRLLPHYTIWTKQYGKRFIYWNGTEPRLCLAETELIKELL 120

Query: 121 GKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECT 180
           GK S VCGRSWLQRQGTKHFIGKGLLMANGE+W HQRHI+APAFMG+KLKSY GFMVECT
Sbjct: 121 GKKSSVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIMAPAFMGDKLKSYAGFMVECT 180

Query: 181 EKMIQSLETELDSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQ 240
            KMIQS+ETEL+SGRTEFEIA+YMKQLTADIISRTEFDT +DKG QIFHLLTLLQRLCAQ
Sbjct: 181 NKMIQSMETELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQ 240

Query: 241 ASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLL 300
           ASRHLCLPGSRF PSKYNREIKSLK EVERLLMEII+SRKDGVEIGRSS YGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLL 300

Query: 301 KEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDEVKQ 360
            EMQ+++    +LNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNP+WQ  VR +V Q
Sbjct: 301 NEMQQRKNISTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQ 360

Query: 361 VCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSIWIP 420
           VCN+   P I+HLSKLTLLNMVINESLRLYPPATVLPRMA ED+K+GD++IPKGLSIWIP
Sbjct: 361 VCNA-RAPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIP 420

Query: 421 VLAIHHSQELWGKDANEFKPERFAAKPFVAGRFIPFAAGPRNCIGQGFALMEAKIILAML 480
           VLAIHHS+ELWGKDAN+F P RF AK F  GRFIPFA GPRNCIGQGFA+MEAKIILAM 
Sbjct: 421 VLAIHHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMF 480

Query: 481 LSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLNS 518
           LSKF FTISDTYRHAPV VLTIQPKYGVQVCL+P  S
Sbjct: 481 LSKFRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 516

BLAST of Sed0021675 vs. ExPASy TrEMBL
Match: A0A5A7V8T6 (Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold508G00410 PE=3 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.6e-246
Identity = 416/481 (86.49%), Postives = 451/481 (93.76%), Query Frame = 0

Query: 40  MAEQGVHGPDPRPLIGNIGDVATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFI 99
           M +QGV GP PRPL+GNI DVA L+AKSTA DMSSI+H++VDRL+PHYTTWTK YGKRFI
Sbjct: 1   MEKQGVRGPVPRPLVGNILDVAALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFI 60

Query: 100 FWNGAEPRLCLAETELIKEVMGKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHI 159
           +WNG EPRLCLAETELIKEV+ K S VCGRSWLQRQGTKHFIGKGLLMANGE+W HQRHI
Sbjct: 61  YWNGMEPRLCLAETELIKEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHI 120

Query: 160 VAPAFMGEKLKSYTGFMVECTEKMIQSLETELDSGRTEFEIAEYMKQLTADIISRTEFDT 219
           VAP+FMG+KLKSY G MVECT+K+IQSLETELDSGRTEFEIA+YMKQLTADIISRTEFD 
Sbjct: 121 VAPSFMGDKLKSYAGLMVECTDKLIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDA 180

Query: 220 SADKGKQIFHLLTLLQRLCAQASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSR 279
           ++DKG QIFHLLTLLQ LCAQASRHLCLPGSRF+PSKYNREIKSLK+EVERLLMEIIQSR
Sbjct: 181 NSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSR 240

Query: 280 KDGVEIGRSSGYGSDLLGMLLKEMQRKRED---GFSLNLQLIMDECKTFFFAGHETTALL 339
           KDGVEIGRS+ YGSDLLGMLL EMQRK++D   GFSLNLQLIMDECKTFFFAGHETTALL
Sbjct: 241 KDGVEIGRSTCYGSDLLGMLLNEMQRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALL 300

Query: 340 LTWTIMLLATNPSWQQKVRDEVKQVCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVL 399
           LTWTIMLLATNP+WQQKVR EV QVC++G+TP +EHLSK TLLNMVINESLRLYPPATVL
Sbjct: 301 LTWTIMLLATNPNWQQKVRHEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVL 360

Query: 400 PRMAFEDIKIGDLEIPKGLSIWIPVLAIHHSQELWGKDANEFKPERFAAKPFVAGRFIPF 459
           PRMAFEDIK+GDLEIPKGLSIWIPVLAIHHS+ELWGKD NEFKPERFA+KPF  GRFIPF
Sbjct: 361 PRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPF 420

Query: 460 AAGPRNCIGQGFALMEAKIILAMLLSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLN 518
           AAGPRNC+GQGFALMEAKIILAMLLSKFSFTISD+YRHAPV+VLTIQPKYGVQV L+P+N
Sbjct: 421 AAGPRNCVGQGFALMEAKIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPIN 480

BLAST of Sed0021675 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 727.6 bits (1877), Expect = 6.9e-210
Identity = 350/513 (68.23%), Postives = 423/513 (82.46%), Query Frame = 0

Query: 7   LTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDVATLVAK 66
           L +LLVIF++++ ++ Y+T++CYWLTPRRI+K M +QGV GP PRPL GNI +++ +V++
Sbjct: 6   LKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISAMVSQ 65

Query: 67  STADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVMGKTSRV 126
           S + D  SI+HD+V RL+PHY  W+K YGKRFI WNG +PRLCL ETELIKE++ K + V
Sbjct: 66  SASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMKHNGV 125

Query: 127 CGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECTEKMIQS 186
            GRSWLQ+QGTK+FIG+GLLMANG++W HQRH+ APAF GE+LK Y   MVECT K+++ 
Sbjct: 126 SGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVER 185

Query: 187 LETELDSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQASRHLC 246
           L  E+  G  E EI E M +LTADIISRT+F +S +KGK++F+ LT+LQR CAQA+RHLC
Sbjct: 186 LRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLC 245

Query: 247 LPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLLKEMQ-R 306
            PGSRF PSKYNREIKSLK EVERLL+EIIQSR+D  E+GRSS +G DLLG+LL EM   
Sbjct: 246 FPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDID 305

Query: 307 KREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDEVKQVCNSG 366
           K  +  + NLQLIMDECKTFFFAGHETTALLLTWT MLLA NP+WQ+KVR+EV++V    
Sbjct: 306 KNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRN 365

Query: 367 STPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSIWIPVLAIH 426
             P ++ LSKLT L+ VINESLRLYPPAT+LPRMAFED+K+GDL IPKGLSIWIPVLAIH
Sbjct: 366 GLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIH 425

Query: 427 HSQELWGKDANEFKPERFAAKPFVAGR-FIPFAAGPRNCIGQGFALMEAKIILAMLLSKF 486
           HS+ELWGKDAN+F PERF  +PF +GR FIPFAAGPRNCIGQ FALMEAKIILA L+SKF
Sbjct: 426 HSEELWGKDANQFNPERFGGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLISKF 485

Query: 487 SFTISDTYRHAPVTVLTIQPKYGVQVCLSPLNS 518
           +FTIS  YRHAP+ VLTI+PKYGVQV L PL S
Sbjct: 486 NFTISKNYRHAPIVVLTIKPKYGVQVILKPLVS 518

BLAST of Sed0021675 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 709.1 bits (1829), Expect = 2.5e-204
Identity = 344/517 (66.54%), Postives = 415/517 (80.27%), Query Frame = 0

Query: 1   MAIPMFLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDV 60
           M + + L  +LVI ++ + ++ Y+++ CY+LTPRRI+K M  QG+ GP PR L GNI D+
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 61  ATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVM 120
           + +++ S ++D SSI+H++V RL+PHY +W+K YGKRFI WNG EPRLCL ETE+IKE++
Sbjct: 61  SKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELL 120

Query: 121 GKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECT 180
            K + V G+SWLQ+QGTK FIG+GLLMANGE W HQRH+ APAF  ++LK Y   MVECT
Sbjct: 121 TKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 180

Query: 181 EKMIQSLETELDSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQ 240
           + M + L  E+     E EI E M++LTADIISRTEF +S DKGK++F LLT+LQRLCAQ
Sbjct: 181 KMMAERLRKEVGE---EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQ 240

Query: 241 ASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLL 300
           A+RHLC PGSRF PSKYNREIKSLK EVERLLMEII SRKD VEIGRSS YG DLLG+LL
Sbjct: 241 ATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLL 300

Query: 301 KEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDEVKQ 360
            +M   +    +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP+WQ  VRDEV+Q
Sbjct: 301 NQMDSNKN---NLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQ 360

Query: 361 VCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSIWIP 420
           VC     P +E LS LT LN VINESLRLYPPAT+LPRMAFEDIK+GDL IPKGLSIWIP
Sbjct: 361 VCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIP 420

Query: 421 VLAIHHSQELWGKDANEFKPERFAAKPFVAGR-FIPFAAGPRNCIGQGFALMEAKIILAM 480
           VLAIHHS ELWG+DANEF PERF  + F + R F+PFAAGPRNCIGQ FA+MEAKIILAM
Sbjct: 421 VLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAM 480

Query: 481 LLSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLN 517
           L+SKFSF IS+ YRHAP+ VLTI+PKYGVQ+ L PL+
Sbjct: 481 LVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Sed0021675 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 360.1 bits (923), Expect = 2.9e-99
Identity = 196/525 (37.33%), Postives = 306/525 (58.29%), Query Frame = 0

Query: 1   MAIPMFLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDV 60
           M + +FL  +++I +  L +I    +   W  P  + +R+  QG+ GP+ R   GN+ ++
Sbjct: 1   MGLVIFLALIVLILIIGL-RIFKAFMILVW-HPFVLTRRLKNQGISGPNYRIFYGNLSEI 60

Query: 61  ATLVAKSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVM 120
             +  +S    +   ++D+  R++PHY  W   YG+ F++WNG EPR+C+++ EL K ++
Sbjct: 61  KKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTML 120

Query: 121 GKTSRVCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECT 180
                   +S  + +  K    KGL+   G +W   R I+ PAF  ++LK  T  MV+CT
Sbjct: 121 SNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCT 180

Query: 181 EKMIQSLETELDSGRTEF-----EIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQ 240
            KM++    E     TE      E+ E  ++LTADII+ + F +S  +G ++F     L+
Sbjct: 181 LKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELK 240

Query: 241 RLCAQASRHLCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRKDGVEIGRSSGYGSDL 300
           R    +   + +PG+++ P+  N  +  L+ +++  +  II SR     +   S YG DL
Sbjct: 241 RCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYGDDL 300

Query: 301 LGMLLKEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVR 360
           LG+LLK    + ++   ++++ I+ EC+TFFF GHETT+ LL WT MLL+ +  WQ+K+R
Sbjct: 301 LGILLKAYNTEGKER-KMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLR 360

Query: 361 DEVKQVCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGL 420
           +E+ + C    TP  E  SKL L+NMVI ESLRLY P + L R A  +IK+GDLEIPKG 
Sbjct: 361 EEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPKGT 420

Query: 421 SIWIPVLAIHHSQELWGKDANEFKPERFAAKPFVAGR----FIPFAAGPRNCIGQGFALM 480
           ++ IP+L +H  + LWG DA++F P RFA     A       + F+ GPR CIGQ F ++
Sbjct: 421 TVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNFVMI 480

Query: 481 EAKIILAMLLSKFSF-TISDTYRHAPVTVLTIQPKYGVQVCLSPL 516
           EAK +L M+L +F F ++ D Y+H PV  +TIQP+YG+ V L PL
Sbjct: 481 EAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Sed0021675 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 356.7 bits (914), Expect = 3.2e-98
Identity = 193/493 (39.15%), Postives = 286/493 (58.01%), Query Frame = 0

Query: 30  WLTPRRIRKRMAEQGVHGPDPRPLIGNIGDVATLVAKSTADDMSSINHDVVDRLMPHYTT 89
           W+  RR +K    QG+ GP  R L GN+ ++  +  ++    +   ++D+V R++PH   
Sbjct: 89  WMLSRRFKK----QGISGPKYRILYGNLREIRKMKNEAKLMVLDPNSNDIVPRVLPHLQQ 148

Query: 90  WTKLYGKRFIFWNGAEPRLCLAETELIKEVMGKTSRVCGRSWLQRQGTKHFIGKGLLMAN 149
           W   YG+ F++W G +PRLC+++ EL K+++        +S  + +  K   G GL+  N
Sbjct: 149 WKSQYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSKSKTKPEILK-LSGNGLIFVN 208

Query: 150 GEEWQHQRHIVAPAFMGEKLKSYTGFMVECTEKMIQSLETELDSGRTE--FEIAEYMKQL 209
           G +W   R I+ PAF  +KLK  T  MV+CT +M    + + +   TE    I+   K+L
Sbjct: 209 GLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKKQRNGVETEQFVLISREFKRL 268

Query: 210 TADIISRTEFDTSADKGKQIFHLLTLLQRLCAQASRHLCLPGSRFYPSKYNREIKSLKVE 269
           TADII+   F +S  +G ++F     LQ+ CA A   L  PG ++ P+  N +I  L ++
Sbjct: 269 TADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSNLQIWKLDMK 328

Query: 270 VERLLMEIIQSRKDGVEIGRSSGYGSDLLGMLLKEMQRKREDGFSLNLQLIMDECKTFFF 329
           V   +  II +R        S  YG+DLLG++L       E    +++  I++ECKTFFF
Sbjct: 329 VNSSIKRIIDARL----TSESKDYGNDLLGIMLTAAS-SNESEKKMSIDEIIEECKTFFF 388

Query: 330 AGHETTALLLTWTIMLLATNPSWQQKVRDEVKQVCNSGSTPLIEHLSKLTLLNMVINESL 389
           AGHETTA LLTW+ MLL+ +  WQ+K+R+EV   C     P  E  SKL L+N V  ESL
Sbjct: 389 AGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAETCSKLKLMNTVFMESL 448

Query: 390 RLYPPATVLPRMAFEDIKIGDLEIPKGLSIWIPVLAIHHSQELWGKDANEFKPERFAAKP 449
           RLY P   L R+A ED+K+G+LEIPKG +I +P+  +H  + +WG DA++F P RFA   
Sbjct: 449 RLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWGSDADKFNPMRFANGL 508

Query: 450 FVAGR----FIPFAAGPRNCIGQGFALMEAKIILAMLLSKFSFTISDTYRHAPVTVLTIQ 509
             A       + F+ GPR CIGQ FA+MEAK +LAM+L +F   +S  Y+HAP   LT+Q
Sbjct: 509 SRAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLNLSADYKHAPADHLTLQ 568

Query: 510 PKYGVQVCLSPLN 517
           P+Y + V L P++
Sbjct: 569 PQYDLPVILEPID 571

BLAST of Sed0021675 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 350.5 bits (898), Expect = 2.3e-96
Identity = 184/521 (35.32%), Postives = 302/521 (57.97%), Query Frame = 0

Query: 6   FLTTLLVIFLSSLAKIAYETVTCYWLTPRRIRKRMAEQGVHGPDPRPLIGNIGDVATLVA 65
           F+  +LV+ +  L+ +  + ++  W  PR+I +  ++QG+ GP     IGN+ ++  ++ 
Sbjct: 9   FIPKVLVLSV-ILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMML 68

Query: 66  KSTADDMSSINHDVVDRLMPHYTTWTKLYGKRFIFWNGAEPRLCLAETELIKEVMGKTSR 125
           K+++  M   +H+++ R++  Y  W K+YG  F+ W G   RL +A+ +LI+E+  K+  
Sbjct: 69  KASSHPM-PFSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSEF 128

Query: 126 VCGRSWLQRQGTKHFIGKGLLMANGEEWQHQRHIVAPAFMGEKLKSYTGFMVECTEKMIQ 185
                       K   G GLL   GE+W H R I++P F  E LK     +++    M+ 
Sbjct: 129 YEKNE--AHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVD 188

Query: 186 SLETEL-DSGRTEFEIAEYMKQLTADIISRTEFDTSADKGKQIFHLLTLLQRLCAQASRH 245
               +L ++G  E ++ E+ + LT D+ISRT F +S + G+ +F L      LCA+A + 
Sbjct: 189 KWSDKLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQK 248

Query: 246 LCLPGSRFYPSKYNREIKSLKVEVERLLMEIIQSRK----DGVEIGRSSGYGSDLLGMLL 305
           + +PG RF+P++ N +   L  E+ + L+++I+ R+    DG           DLLG+++
Sbjct: 249 VFIPGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI 308

Query: 306 KEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQQKVRDEVKQ 365
           +          ++ +Q I++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ K RDEV +
Sbjct: 309 QAK--------NVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLR 368

Query: 366 VCNSGSTPLIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKIGDLEIPKGLSIWIP 425
           VC S   P  +H+ KL  L+M++NESLRLYPP     R A  D+K+G  +IP G  + IP
Sbjct: 369 VCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIP 428

Query: 426 VLAIHHSQELWGKDANEFKPERFA-AKPFVAGR---FIPFAAGPRNCIGQGFALMEAKII 485
           ++A+HH Q +WG D NEF P RFA   P  A     FIPF  G R CIGQ  A+++AK+ 
Sbjct: 429 IIAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLT 488

Query: 486 LAMLLSKFSFTISDTYRHAPVTVLTIQPKYGVQVCLSPLNS 518
           LA+++ +F+F ++ TY+HAP  ++ + P++G  +    L +
Sbjct: 489 LAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLTN 517

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038905687.17.0e-26586.49cytokinin hydroxylase-like [Benincasa hispida][more]
XP_008461587.11.2e-26486.15PREDICTED: cytokinin hydroxylase-like [Cucumis melo][more]
XP_004145660.11.9e-26285.58cytokinin hydroxylase [Cucumis sativus] >KGN57750.1 hypothetical protein Csa_009... [more]
XP_022983651.13.2e-24983.59cytokinin hydroxylase-like [Cucurbita maxima][more]
XP_023528555.11.0e-24782.15cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FF189.7e-20968.23Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1[more]
Q9ZW953.6e-20366.54Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1[more]
Q9ASR34.1e-9837.33Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1[more]
F4IK454.5e-9739.15Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1[more]
O487863.2e-9535.32Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CGC05.8e-26586.15cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=3 SV=1[more]
A0A0A0L6Y69.2e-26385.58Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=3 SV=1[more]
A0A6J1J2Y11.5e-24983.59cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111482203 PE=3 SV=1[more]
A0A6J1F8S18.4e-24882.40cytokinin hydroxylase OS=Cucurbita moschata OX=3662 GN=LOC111441866 PE=3 SV=1[more]
A0A5A7V8T61.6e-24686.49Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
Match NameE-valueIdentityDescription
AT5G38450.16.9e-21068.23cytochrome P450, family 735, subfamily A, polypeptide 1 [more]
AT1G67110.12.5e-20466.54cytochrome P450, family 735, subfamily A, polypeptide 2 [more]
AT2G46960.22.9e-9937.33cytochrome P450, family 709, subfamily B, polypeptide 1 [more]
AT2G46950.13.2e-9839.15cytochrome P450, family 709, subfamily B, polypeptide 2 [more]
AT2G26710.12.3e-9635.32Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 261..281
NoneNo IPR availableCOILSCoilCoilcoord: 177..197
NoneNo IPR availablePANTHERPTHR24282:SF186CYTOCHROME P450 FAMILY PROTEINcoord: 5..511
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 5..511
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 91..110
score: 25.76
coord: 463..486
score: 34.59
coord: 453..463
score: 52.03
coord: 317..334
score: 29.73
coord: 337..363
score: 28.74
coord: 421..445
score: 24.55
IPR001128Cytochrome P450PRINTSPR00385P450coord: 328..345
score: 40.88
coord: 463..474
score: 45.39
coord: 454..463
score: 57.09
coord: 381..392
score: 46.75
IPR001128Cytochrome P450PFAMPF00067p450coord: 87..492
e-value: 7.1E-87
score: 292.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 29..516
e-value: 5.9E-114
score: 383.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 79..514

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0021675.1Sed0021675.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen