Sed0021548 (gene) Chayote v1

BLAST of Sed0021548 vs. NCBI nr
Match: XP_022144258.1 (uncharacterized membrane protein At1g75140-like [Momordica charantia])

HSP 1 Score: 1064.7 bits (2752), Expect = 3.2e-307
Identity = 544/652 (83.44%), Postives = 587/652 (90.03%), Query Frame = 0

Query: 1   MANPLKGKLLLISFLF------VSRVFLCTSSSFEQN----ESESEVPFEIRQYDLLKNL 60
           MANP KGKLLLI FLF      VSRVF+C+SSSFEQN    ESESEVPFEIRQ DLLKNL
Sbjct: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60

Query: 61  EELVRNLSDVVSKLELRLSDFPTPVVTDKNLKSEPS-RVVAEVVSLGGGKKDSLH---DG 120
           EELVRNLSDVVSKLE+RLSD PT V+ DK   ++ S RVV EVVSLGG K+D L+   DG
Sbjct: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSLGGRKEDGLNDGGDG 120

Query: 121 GLDNRVQDDGGTARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGNSKYVA 180
           GL+++++ DG   RAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLP RDFEG+SKYV 
Sbjct: 121 GLESKIR-DGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVV 180

Query: 181 VGDERGRVYVFMRNGDVAVELRTVSDSPITTMLSYMSIYKNETLLVTGHKNGAILMHRIW 240
           VGDE+GRVYVF RNGDVAVELRT S+SPI TMLSYMSIYKNETLLVTGHKNGAILMHRIW
Sbjct: 181 VGDEKGRVYVFTRNGDVAVELRTKSESPIMTMLSYMSIYKNETLLVTGHKNGAILMHRIW 240

Query: 241 EGSNGEELNSLFMEYVVEFVATNGREEESPISILEVHHVGRTRYILSSDVRGKIKVFREN 300
           E SNGEE +SLFME+VVEFVAT+GRE+ESPISILEVHHVGRTRYILSSDVRGKIKVFREN
Sbjct: 241 ERSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFREN 300

Query: 301 GAVYGSVVPMSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDTM 360
           G VYGSV+P S+PIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLARNYVFD  
Sbjct: 301 GTVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDAT 360

Query: 361 ERSKAYGFTSDGDLINVMLLGDIINFKCRVRSKRKFELDEPLVFQALKGYLLVINNKKVH 420
           ERSKAYGFTS GDLI+V+LLGDI+NFKCRVRSKRKFELDEPLVFQA+KGYLLVI+N+KVH
Sbjct: 361 ERSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVH 420

Query: 421 IFNVSSQHYVRVGAPRHLFSVGLTEIRSSFMNYQNFDPKIEGKFIPLISSDHEKLIVLGL 480
           IFNVSSQHY RVGAPR LFS  + EIRSSF+NYQN D + EGKF+PLISSD EKL+VLGL
Sbjct: 421 IFNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGL 480

Query: 481 GGGYVGMYRSNLSIFKGEFNTMLWTSPVLLFVLFLFGAWQFFAKKKEALTSWGPDDPFTA 540
           GGGYVGMYRSNL IFKGEFNTMLWTSPVL F+LFLFGAW FFAKKKEALTSWGPDDPFTA
Sbjct: 481 GGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTA 540

Query: 541 TKPTTGAPLGIGSRERASFIDNSSRSTDMMDLRGGSGLRGPPRRYGSPTGYTGGATSSFR 600
           T PTTGAPLG G+ ER+SFID  SRSTDMMDLRGGSGLRGPPRRYGSPTGY GGAT+SFR
Sbjct: 541 TSPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGATNSFR 600

Query: 601 PATTNAHSSRPDAVDPNYRAASELKFRSSPLEPPGFPKRREPLFASNQVVNQ 639
           P TT+ H+SRP AVDPNYRAASELKFR SPLEPPGFPKRREPLFA+NQVV++
Sbjct: 601 PPTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVDE 651

BLAST of Sed0021548 vs. NCBI nr
Match: XP_038885478.1 (uncharacterized membrane protein At1g75140-like [Benincasa hispida])

HSP 1 Score: 1063.9 bits (2750), Expect = 5.4e-307
Identity = 541/656 (82.47%), Postives = 586/656 (89.33%), Query Frame = 0

Query: 1   MANPLKGKLLLISFLF------VSRVFLCTSSSFEQNESESEVPFEIRQYDLLKNLEELV 60
           MAN  KGKLL I FLF      VSRVF+C+SSSFE N+SE EVPFEI+QY+LLKNLEELV
Sbjct: 1   MANLRKGKLLFIYFLFVFSFPHVSRVFVCSSSSFELNQSEIEVPFEIKQYNLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDFPTPVVTDKNLKSEPSRVVAEVVSLGG----GKKDSLH---DGG 120
           RNLSDVVSKLELRLSD PT V  DK       RVV+EVV LGG    GK+D L+   DGG
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVHKDKLNTDASRRVVSEVVGLGGVKRKGKEDGLNDGGDGG 120

Query: 121 LDNRVQDDGGTARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGNSKYVAV 180
           LDN++Q DGG  RAVSVTKYSPFWSERFHFLSAVKL+SDATCINVLP+RDFEG+SKYVAV
Sbjct: 121 LDNKIQ-DGGRMRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPIRDFEGHSKYVAV 180

Query: 181 GDERGRVYVFMRNGDVAVELRTVSDSPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240
           GDE+GR+YVF+RNGDVA+EL TVS+SPIT MLSY SIYKNETLLVTGH NGAILMHRIWE
Sbjct: 181 GDEKGRIYVFVRNGDVAIELPTVSESPITAMLSYTSIYKNETLLVTGHNNGAILMHRIWE 240

Query: 241 GSNGEELNSLFMEYVVEFVATNGREEESPISILEVHHVGRTRYILSSDVRGKIKVFRENG 300
           G NGEELNS+FME+VVEFVAT+ RE++SPISILEVHHVGRTRYILSSD RGKIKVF+E+G
Sbjct: 241 GLNGEELNSIFMEHVVEFVATDSREDDSPISILEVHHVGRTRYILSSDFRGKIKVFKEDG 300

Query: 301 AVYGSVVPMSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDTME 360
            VYGSV  MSRPIAFLKQRLLFLTE+GAGSLDLRSMKLRE+ CEGLNYSLARNYVFD  E
Sbjct: 301 TVYGSVTLMSRPIAFLKQRLLFLTESGAGSLDLRSMKLREAGCEGLNYSLARNYVFDATE 360

Query: 361 RSKAYGFTSDGDLINVMLLGDIINFKCRVRSKRKFELDEPLVFQALKGYLLVINNKKVHI 420
           RSKAYGFTSDGDLI+V+LLGDI+NFKCRVRSKRKFELDEPLVFQA+KGYLLVI+N+KVH+
Sbjct: 361 RSKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHV 420

Query: 421 FNVSSQHYVRVGAPRHLFSVGLTEIRSSFMNYQNFDPKIEGKFIPLISSDHEKLIVLGLG 480
           FNVSSQHYVRV APR LFS GL EIRSSF+NYQN + + EGKFIPLISSDHEKL+VLGLG
Sbjct: 421 FNVSSQHYVRVAAPRLLFSAGLDEIRSSFLNYQNLNLESEGKFIPLISSDHEKLVVLGLG 480

Query: 481 GGYVGMYRSNLSIFKGEFNTMLWTSPVLLFVLFLFGAWQFFAKKKEALTSWGPDDPFTAT 540
           GGYVGMYRSNL IFKGEFN+MLWTSPVL F+LFLFGAWQFFAKKKEALTSWGPDDPFTAT
Sbjct: 481 GGYVGMYRSNLPIFKGEFNSMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTAT 540

Query: 541 KPTTGAPLGIGSRERASFIDNSSRSTDMMDLRGGSGLRGPPRRYGSPTGYTGGATSSFRP 600
            PTTG PLG GS ERASFID  SRSTDMMDLRGG G+RGPPR YGSPTGY GGATSSFRP
Sbjct: 541 SPTTGVPLGTGSSERASFIDTPSRSTDMMDLRGGGGIRGPPRHYGSPTGYPGGATSSFRP 600

Query: 601 ATTNAHSSRPDAVDPNYRAASELKFRSSPLEPPGFPKRREPLFASNQVVNQVVDES 644
            TT+ HSSRP AVDP+YRAASELKFR SPLEPPGFPKRREPLFA+NQVVNQ+VDES
Sbjct: 601 PTTSDHSSRPAAVDPSYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQMVDES 655

BLAST of Sed0021548 vs. NCBI nr
Match: XP_022962086.1 (uncharacterized membrane protein At1g75140-like [Cucurbita moschata])

HSP 1 Score: 1056.2 bits (2730), Expect = 1.1e-304
Identity = 545/667 (81.71%), Postives = 585/667 (87.71%), Query Frame = 0

Query: 1   MANPLKGKLLLISFLF------VSRVFLCTSSSFEQNESESEVPFEIRQYDLLKNLEELV 60
           MANP KGKLL I FLF      VSR F+C SSS E N+SE+EVP+E RQYD+LKNLEELV
Sbjct: 1   MANPWKGKLLFIYFLFVFGIPYVSRDFVCCSSSLELNQSETEVPYETRQYDVLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDFPTPVVTDKNLKSEPS-RVVAEVVSLGGGKKDSLHDGG---LDN 120
           RNLSDVVSKLELRLS+ PT V  D  L ++ S RVV EVVSLGGGK+D L+DGG   LD 
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKEDGLNDGGHGALDT 120

Query: 121 RVQDDGGTARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGNSKYVAVGDE 180
           RVQ DGG  RAVSVTKYSPFWSERFHFLSAVKL+SDAT INVLPLRDFEG+SKYVAVGDE
Sbjct: 121 RVQ-DGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSKYVAVGDE 180

Query: 181 RGRVYVFMRNGDVAVELRTVSDSPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN 240
            GR+YVF+RNGDVAVELRTVS SPIT MLSYMSIYKNETLLVTGH++GAI MHRIWEGSN
Sbjct: 181 TGRIYVFVRNGDVAVELRTVSGSPITAMLSYMSIYKNETLLVTGHESGAISMHRIWEGSN 240

Query: 241 GEELNSLFMEYVVEFVATNGREEESPISILEVHHVGRTRYILSSDVRGKIKVFRENGAVY 300
           GEE NS+FME+V+EFV T+GREE+SPISILEVHHVGRTRYILSSDV+GKIKVFRE+G VY
Sbjct: 241 GEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDGTVY 300

Query: 301 GSVVPMSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDTMERSK 360
           GSV+P SRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFD  ERSK
Sbjct: 301 GSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSK 360

Query: 361 AYGFTSDGDLINVMLLGDIINFKCRVRSKRKFELDEPLVFQALKGYLLVINNKKVHIFNV 420
           AYGFTSDGDLI+VMLLGDI+NFKCRVRSK+KFELDEPL FQA+KGYLLVI ++KVH+FNV
Sbjct: 361 AYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSEKVHVFNV 420

Query: 421 SSQHYVRVGAPRHLFSVGLTEIRSSFMNYQNFDPKIEGKFIPLISSDHEKLIVLGLGGGY 480
           SSQHYVRVGAPR LFS  L EIRSSF+N QN D + +GKFIPLISSD EKL+VLGLGGGY
Sbjct: 421 SSQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVVLGLGGGY 480

Query: 481 VGMYRSNLSIFKGEFNTMLWTSPVLLFVLFLFGAWQFFAKKKEALTSWGPDDPFTATKPT 540
           +GMYRSNL I+KGEFNTMLWTSPVL F+LFLFGAWQFFAKKKEALTSWGPDDPFTAT PT
Sbjct: 481 IGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPT 540

Query: 541 TGAPLGIGSRE--------------RASFIDNSSRSTDMMDLRGGSGLRGPPRRYGSPTG 600
           TGAPLG GS E              R SFID  SRSTDMMDLRGGSGLRGPPRRYGSPTG
Sbjct: 541 TGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTG 600

Query: 601 YTGGATSSFRPATTNAHSSRPDAVDPNYRAASELKFRSSPLEPPGFPKRREPLFASNQVV 644
           Y GG TSSFRPATT+ HSSR  AVDPNYRAASELKFR SPLEPPGFPKRREPLFA+NQVV
Sbjct: 601 YPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVV 660

BLAST of Sed0021548 vs. NCBI nr
Match: XP_023546241.1 (uncharacterized membrane protein At1g75140-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1055.0 bits (2727), Expect = 2.5e-304
Identity = 543/667 (81.41%), Postives = 586/667 (87.86%), Query Frame = 0

Query: 1   MANPLKGKLLLISFLF------VSRVFLCTSSSFEQNESESEVPFEIRQYDLLKNLEELV 60
           MANP KGKLL I FLF      VSR F+C SSS E N+SE+EVPFE RQYD+LKNLEELV
Sbjct: 1   MANPRKGKLLFIYFLFVFGIPYVSRDFVCCSSSLELNQSETEVPFETRQYDVLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDFPTPVVTDKNLKSEPS-RVVAEVVSLGGGKKDSLHDGG---LDN 120
           RNLSDVVSKLELRLS+ PT V  D  L ++ S RVV EVVSLGGGK+D L+DGG   LD 
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKQDGLNDGGDGDLDT 120

Query: 121 RVQDDGGTARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGNSKYVAVGDE 180
           R+Q DGG  RAVSVTKYSPFWSERFHF+SAVKL+SDAT INVLPLRDFEG+SKYVAVGDE
Sbjct: 121 RIQ-DGGRGRAVSVTKYSPFWSERFHFMSAVKLESDATSINVLPLRDFEGHSKYVAVGDE 180

Query: 181 RGRVYVFMRNGDVAVELRTVSDSPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN 240
            GR+YVF+RNGDVAVELRT+S SPIT MLSYMSIYKNETLLVTGH++GAI MHRIWEGSN
Sbjct: 181 TGRIYVFVRNGDVAVELRTMSGSPITAMLSYMSIYKNETLLVTGHESGAISMHRIWEGSN 240

Query: 241 GEELNSLFMEYVVEFVATNGREEESPISILEVHHVGRTRYILSSDVRGKIKVFRENGAVY 300
           GEE NS+FME+V+EFV T+GREE+SPISILEVHHVGRTRYILSSDV+GKIKVFRE+G VY
Sbjct: 241 GEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDGTVY 300

Query: 301 GSVVPMSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDTMERSK 360
           GSV+P SRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFD  ERSK
Sbjct: 301 GSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSK 360

Query: 361 AYGFTSDGDLINVMLLGDIINFKCRVRSKRKFELDEPLVFQALKGYLLVINNKKVHIFNV 420
           AYGFTSDGDLI+VMLLGDI+NFKCRVRSK+KFELDEPL+FQA+KGYLLVI ++KVH+FNV
Sbjct: 361 AYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLLFQAVKGYLLVIGSEKVHVFNV 420

Query: 421 SSQHYVRVGAPRHLFSVGLTEIRSSFMNYQNFDPKIEGKFIPLISSDHEKLIVLGLGGGY 480
           SSQHYVRVGAPR LFS  L EIRSSF+N QN D + +GKFIPLISSD EKL+VLGLGGGY
Sbjct: 421 SSQHYVRVGAPRLLFSARLDEIRSSFLNNQNSDLESQGKFIPLISSDREKLVVLGLGGGY 480

Query: 481 VGMYRSNLSIFKGEFNTMLWTSPVLLFVLFLFGAWQFFAKKKEALTSWGPDDPFTATKPT 540
           +GMYRSNL I+KGEFNTMLWTSPVL F+LFLFGAWQFFAKKKEALTSWGPDDPFTAT PT
Sbjct: 481 IGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPT 540

Query: 541 TGAPLGIGSRE--------------RASFIDNSSRSTDMMDLRGGSGLRGPPRRYGSPTG 600
           TGAPLG GS E              R SFID  SRSTDMMDLRGGSGLRGPPRRYGSPTG
Sbjct: 541 TGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTG 600

Query: 601 YTGGATSSFRPATTNAHSSRPDAVDPNYRAASELKFRSSPLEPPGFPKRREPLFASNQVV 644
           Y GG TSSFRPATT+ HSSR  AVDPNYRAASELKFR SPLEPPGFPKRREPLFA+NQVV
Sbjct: 601 YPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVV 660

BLAST of Sed0021548 vs. NCBI nr
Match: XP_022996718.1 (uncharacterized membrane protein At1g75140-like [Cucurbita maxima])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.8e-303
Identity = 543/667 (81.41%), Postives = 584/667 (87.56%), Query Frame = 0

Query: 1   MANPLKGKLLLISFLF------VSRVFLCTSSSFEQNESESEVPFEIRQYDLLKNLEELV 60
           MANP KGKLL I FLF      VSR F+C SSSFE N+SE+EVPFE RQYDLLKNLEELV
Sbjct: 1   MANPRKGKLLFIYFLFVFGIPHVSRDFVCCSSSFELNQSETEVPFETRQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDFPTPVVTDKNLKSEPS-RVVAEVVSLGGGKKDSLHDGG---LDN 120
           RNLSDVVSKLELRLS+ PT V  D  L ++ S RVV EVVSLGGGK+D L+DGG   LD 
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKEDGLNDGGDGALDT 120

Query: 121 RVQDDGGTARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGNSKYVAVGDE 180
           ++Q DGG  RAVSVTKYSPFWSERFHFLSAVKL+SDAT INVLPLRDFEG+SKYVAVGDE
Sbjct: 121 KIQ-DGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSKYVAVGDE 180

Query: 181 RGRVYVFMRNGDVAVELRTVSDSPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN 240
            GR+YVF+RNGDVAVELRT+S SPIT  LSYMSIYKNETLLVTGH++GAI M RIWEGSN
Sbjct: 181 TGRIYVFVRNGDVAVELRTMSGSPITATLSYMSIYKNETLLVTGHESGAISMLRIWEGSN 240

Query: 241 GEELNSLFMEYVVEFVATNGREEESPISILEVHHVGRTRYILSSDVRGKIKVFRENGAVY 300
           GEE NS+FME+V+EFV T+GREE+SPISILEVHHVGRTRYILSSDV+GKIKVFRE+G VY
Sbjct: 241 GEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDGTVY 300

Query: 301 GSVVPMSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDTMERSK 360
           GSV+P SRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFD  ERSK
Sbjct: 301 GSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSK 360

Query: 361 AYGFTSDGDLINVMLLGDIINFKCRVRSKRKFELDEPLVFQALKGYLLVINNKKVHIFNV 420
           AYGFTSDGDLI+VMLLGDI+NFKCRVRSK+KFELDEPL FQA+KGYLLVI ++KVH+FNV
Sbjct: 361 AYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSEKVHVFNV 420

Query: 421 SSQHYVRVGAPRHLFSVGLTEIRSSFMNYQNFDPKIEGKFIPLISSDHEKLIVLGLGGGY 480
           SSQHYVRVGAPR LFS  L EIRSSF+N QN D + +GKFIPLISSD EKL+VLGLGGGY
Sbjct: 421 SSQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVVLGLGGGY 480

Query: 481 VGMYRSNLSIFKGEFNTMLWTSPVLLFVLFLFGAWQFFAKKKEALTSWGPDDPFTATKPT 540
           +GMYRSNL I+KGEFNTMLWTSPVL F+LFLFGAWQFFAKKKEALTSWGPDDPFTAT PT
Sbjct: 481 IGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPT 540

Query: 541 TGAPLGIGSRE--------------RASFIDNSSRSTDMMDLRGGSGLRGPPRRYGSPTG 600
           TGAPLG GS E              R SFID  SRSTDMMDLRGGSGLRGPPRRYGSPTG
Sbjct: 541 TGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTG 600

Query: 601 YTGGATSSFRPATTNAHSSRPDAVDPNYRAASELKFRSSPLEPPGFPKRREPLFASNQVV 644
           Y GG TSSFRPATT+ HSSR  AVDPNYRAASELKFR SPLEPPGFPKRREPLFA+NQVV
Sbjct: 601 YPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVV 660

BLAST of Sed0021548 vs. ExPASy Swiss-Prot
Match: Q9FRK5 (Uncharacterized membrane protein At1g75140 OS=Arabidopsis thaliana OX=3702 GN=At1g75140 PE=1 SV=3)

HSP 1 Score: 625.5 bits (1612), Expect = 6.4e-178
Identity = 352/666 (52.85%), Postives = 454/666 (68.17%), Query Frame = 0

Query: 1   MANPLKGKLLLISFLFVSRVFLCTSSSF-EQNESESE-VPFE--------------IRQY 60
           MA+   GK     F FVS + L  S S+ +   SES+ +P+E               RQ 
Sbjct: 1   MADSQNGKSAFF-FFFVSLILLFLSPSYSDVTASESDPIPYENSDASPGVVTSSESDRQG 60

Query: 61  DLLKNLEELVRNLSDVVSKLELRLSDFPTPVVTDKNLKSEPSRVVAEVVSLGGGKKDSLH 120
             L  LEELVRNL+++V++L+ +LS+ P  V      K E +R             D + 
Sbjct: 61  VSLHRLEELVRNLTELVARLDAKLSETPFKV------KKEITR-------------DEIE 120

Query: 121 DGGLDNRVQDDGGTARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGNSKY 180
           +             A+A SVTKYSPFWSERF F SAVKLDS+ATCINVLP RD EG SKY
Sbjct: 121 E------------KAKAFSVTKYSPFWSERFEFTSAVKLDSEATCINVLPFRDHEGLSKY 180

Query: 181 VAVGDERGRVYVFMRNGDVAVELRTVSDSPITTMLSYMSIYKNETLLVTGHKNGAILMHR 240
            AVGD  GRV+VF+RNGDV VE  T  DSPIT M+SYMS+YKNE+ +VTGH++G IL+HR
Sbjct: 181 FAVGDSSGRVFVFLRNGDVLVEFFTTCDSPITAMVSYMSVYKNESFVVTGHQSGVILLHR 240

Query: 241 IWEGSNGEELNSLFMEYVVEFVATNGREEESPISILEVHHVGRTRYILSSDVRGKIKVFR 300
           + EGS GE+LNS  ME V +F   +G E+   +++LEVHHVGR RYIL++D+ GK+ VF 
Sbjct: 241 LREGSIGEDLNSAVMENVGKF---DGTEDGLQVTLLEVHHVGRVRYILATDLSGKLTVFT 300

Query: 301 ENGAVYGSVVPMSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFD 360
           EN  VYGSV P SRP+ FLKQRLLFLTETGAGSLDLRSMK+RESECEGLN+SLAR+YVFD
Sbjct: 301 ENRTVYGSVSPTSRPLVFLKQRLLFLTETGAGSLDLRSMKIRESECEGLNHSLARSYVFD 360

Query: 361 TMERSKAYGFTSDGDLINVMLLGDIINFKCRVRSKRKFELDEPLVFQALKGYLLVINNKK 420
             ER+KAYGFTS+G++I+V+LLGDI+NFKCRVRSK+K +++EP+  QA+KGYLL++N +K
Sbjct: 361 ASERAKAYGFTSEGEIIHVLLLGDIMNFKCRVRSKKKVQMEEPVALQAIKGYLLIVNQEK 420

Query: 421 VHIFNVSSQHYVRVGAPRHLFSVGLTEIRSSFMNY----QNFDPKIEGKFIPLISSDHEK 480
           V ++NVS+QHYVR   PR LF   L +IRS+F+++    +  D +   K  PLI+SD EK
Sbjct: 421 VFVYNVSTQHYVRTTGPRLLFPAALEDIRSTFLSHRESTKTTDHQKLEKVTPLIASDREK 480

Query: 481 LIVLGLGGGYVGMYRSNLSIFKGEFNTMLWTSPVLLFVLFLFGAWQFFAKKKEALTSWGP 540
           L+V+GLG GYV  Y+S L I K EFNTMLW+SPV  F+LFLFGAW FF+KKKE+LT+WGP
Sbjct: 481 LLVMGLGDGYVATYKSKLPISKAEFNTMLWSSPVFFFILFLFGAWHFFSKKKESLTAWGP 540

Query: 541 DDPFTATKPTTGAPLGIGSRERASFIDNSSRSTDMMDLRGGSGLRGPPRRYGSPTGY-TG 600
           DDPF++T  T  +     ++  ++F +++ R+ D MDLR         RRY SP+ Y  G
Sbjct: 541 DDPFSST--TMSSSSTTTAQNSSAFSESTRRNDDHMDLR---------RRYVSPSRYPPG 600

Query: 601 GATSSFRPATTNAHSSRPDAVDPNYR-AASELKFR-SSPLEPPGFPKRREPLFASNQVVN 644
            AT ++R   +N  SSR      NYR  A E+K+R  S L+  GF KRRE LF +N+ ++
Sbjct: 601 AATGAYRSVGSNDPSSRAPVETTNYRTTAQEMKYRGGSGLDSGGFGKRRESLFGNNKALD 617

BLAST of Sed0021548 vs. ExPASy TrEMBL
Match: A0A6J1CSU7 (uncharacterized membrane protein At1g75140-like OS=Momordica charantia OX=3673 GN=LOC111013986 PE=4 SV=1)

HSP 1 Score: 1064.7 bits (2752), Expect = 1.5e-307
Identity = 544/652 (83.44%), Postives = 587/652 (90.03%), Query Frame = 0

Query: 1   MANPLKGKLLLISFLF------VSRVFLCTSSSFEQN----ESESEVPFEIRQYDLLKNL 60
           MANP KGKLLLI FLF      VSRVF+C+SSSFEQN    ESESEVPFEIRQ DLLKNL
Sbjct: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60

Query: 61  EELVRNLSDVVSKLELRLSDFPTPVVTDKNLKSEPS-RVVAEVVSLGGGKKDSLH---DG 120
           EELVRNLSDVVSKLE+RLSD PT V+ DK   ++ S RVV EVVSLGG K+D L+   DG
Sbjct: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSLGGRKEDGLNDGGDG 120

Query: 121 GLDNRVQDDGGTARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGNSKYVA 180
           GL+++++ DG   RAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLP RDFEG+SKYV 
Sbjct: 121 GLESKIR-DGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVV 180

Query: 181 VGDERGRVYVFMRNGDVAVELRTVSDSPITTMLSYMSIYKNETLLVTGHKNGAILMHRIW 240
           VGDE+GRVYVF RNGDVAVELRT S+SPI TMLSYMSIYKNETLLVTGHKNGAILMHRIW
Sbjct: 181 VGDEKGRVYVFTRNGDVAVELRTKSESPIMTMLSYMSIYKNETLLVTGHKNGAILMHRIW 240

Query: 241 EGSNGEELNSLFMEYVVEFVATNGREEESPISILEVHHVGRTRYILSSDVRGKIKVFREN 300
           E SNGEE +SLFME+VVEFVAT+GRE+ESPISILEVHHVGRTRYILSSDVRGKIKVFREN
Sbjct: 241 ERSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFREN 300

Query: 301 GAVYGSVVPMSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDTM 360
           G VYGSV+P S+PIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLARNYVFD  
Sbjct: 301 GTVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDAT 360

Query: 361 ERSKAYGFTSDGDLINVMLLGDIINFKCRVRSKRKFELDEPLVFQALKGYLLVINNKKVH 420
           ERSKAYGFTS GDLI+V+LLGDI+NFKCRVRSKRKFELDEPLVFQA+KGYLLVI+N+KVH
Sbjct: 361 ERSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVH 420

Query: 421 IFNVSSQHYVRVGAPRHLFSVGLTEIRSSFMNYQNFDPKIEGKFIPLISSDHEKLIVLGL 480
           IFNVSSQHY RVGAPR LFS  + EIRSSF+NYQN D + EGKF+PLISSD EKL+VLGL
Sbjct: 421 IFNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGL 480

Query: 481 GGGYVGMYRSNLSIFKGEFNTMLWTSPVLLFVLFLFGAWQFFAKKKEALTSWGPDDPFTA 540
           GGGYVGMYRSNL IFKGEFNTMLWTSPVL F+LFLFGAW FFAKKKEALTSWGPDDPFTA
Sbjct: 481 GGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTA 540

Query: 541 TKPTTGAPLGIGSRERASFIDNSSRSTDMMDLRGGSGLRGPPRRYGSPTGYTGGATSSFR 600
           T PTTGAPLG G+ ER+SFID  SRSTDMMDLRGGSGLRGPPRRYGSPTGY GGAT+SFR
Sbjct: 541 TSPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGATNSFR 600

Query: 601 PATTNAHSSRPDAVDPNYRAASELKFRSSPLEPPGFPKRREPLFASNQVVNQ 639
           P TT+ H+SRP AVDPNYRAASELKFR SPLEPPGFPKRREPLFA+NQVV++
Sbjct: 601 PPTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVDE 651

BLAST of Sed0021548 vs. ExPASy TrEMBL
Match: A0A6J1HC37 (uncharacterized membrane protein At1g75140-like OS=Cucurbita moschata OX=3662 GN=LOC111462650 PE=4 SV=1)

HSP 1 Score: 1056.2 bits (2730), Expect = 5.5e-305
Identity = 545/667 (81.71%), Postives = 585/667 (87.71%), Query Frame = 0

Query: 1   MANPLKGKLLLISFLF------VSRVFLCTSSSFEQNESESEVPFEIRQYDLLKNLEELV 60
           MANP KGKLL I FLF      VSR F+C SSS E N+SE+EVP+E RQYD+LKNLEELV
Sbjct: 1   MANPWKGKLLFIYFLFVFGIPYVSRDFVCCSSSLELNQSETEVPYETRQYDVLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDFPTPVVTDKNLKSEPS-RVVAEVVSLGGGKKDSLHDGG---LDN 120
           RNLSDVVSKLELRLS+ PT V  D  L ++ S RVV EVVSLGGGK+D L+DGG   LD 
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKEDGLNDGGHGALDT 120

Query: 121 RVQDDGGTARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGNSKYVAVGDE 180
           RVQ DGG  RAVSVTKYSPFWSERFHFLSAVKL+SDAT INVLPLRDFEG+SKYVAVGDE
Sbjct: 121 RVQ-DGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSKYVAVGDE 180

Query: 181 RGRVYVFMRNGDVAVELRTVSDSPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN 240
            GR+YVF+RNGDVAVELRTVS SPIT MLSYMSIYKNETLLVTGH++GAI MHRIWEGSN
Sbjct: 181 TGRIYVFVRNGDVAVELRTVSGSPITAMLSYMSIYKNETLLVTGHESGAISMHRIWEGSN 240

Query: 241 GEELNSLFMEYVVEFVATNGREEESPISILEVHHVGRTRYILSSDVRGKIKVFRENGAVY 300
           GEE NS+FME+V+EFV T+GREE+SPISILEVHHVGRTRYILSSDV+GKIKVFRE+G VY
Sbjct: 241 GEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDGTVY 300

Query: 301 GSVVPMSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDTMERSK 360
           GSV+P SRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFD  ERSK
Sbjct: 301 GSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSK 360

Query: 361 AYGFTSDGDLINVMLLGDIINFKCRVRSKRKFELDEPLVFQALKGYLLVINNKKVHIFNV 420
           AYGFTSDGDLI+VMLLGDI+NFKCRVRSK+KFELDEPL FQA+KGYLLVI ++KVH+FNV
Sbjct: 361 AYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSEKVHVFNV 420

Query: 421 SSQHYVRVGAPRHLFSVGLTEIRSSFMNYQNFDPKIEGKFIPLISSDHEKLIVLGLGGGY 480
           SSQHYVRVGAPR LFS  L EIRSSF+N QN D + +GKFIPLISSD EKL+VLGLGGGY
Sbjct: 421 SSQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVVLGLGGGY 480

Query: 481 VGMYRSNLSIFKGEFNTMLWTSPVLLFVLFLFGAWQFFAKKKEALTSWGPDDPFTATKPT 540
           +GMYRSNL I+KGEFNTMLWTSPVL F+LFLFGAWQFFAKKKEALTSWGPDDPFTAT PT
Sbjct: 481 IGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPT 540

Query: 541 TGAPLGIGSRE--------------RASFIDNSSRSTDMMDLRGGSGLRGPPRRYGSPTG 600
           TGAPLG GS E              R SFID  SRSTDMMDLRGGSGLRGPPRRYGSPTG
Sbjct: 541 TGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTG 600

Query: 601 YTGGATSSFRPATTNAHSSRPDAVDPNYRAASELKFRSSPLEPPGFPKRREPLFASNQVV 644
           Y GG TSSFRPATT+ HSSR  AVDPNYRAASELKFR SPLEPPGFPKRREPLFA+NQVV
Sbjct: 601 YPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVV 660

BLAST of Sed0021548 vs. ExPASy TrEMBL
Match: A0A6J1KBU3 (uncharacterized membrane protein At1g75140-like OS=Cucurbita maxima OX=3661 GN=LOC111491879 PE=4 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.3e-303
Identity = 543/667 (81.41%), Postives = 584/667 (87.56%), Query Frame = 0

Query: 1   MANPLKGKLLLISFLF------VSRVFLCTSSSFEQNESESEVPFEIRQYDLLKNLEELV 60
           MANP KGKLL I FLF      VSR F+C SSSFE N+SE+EVPFE RQYDLLKNLEELV
Sbjct: 1   MANPRKGKLLFIYFLFVFGIPHVSRDFVCCSSSFELNQSETEVPFETRQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDFPTPVVTDKNLKSEPS-RVVAEVVSLGGGKKDSLHDGG---LDN 120
           RNLSDVVSKLELRLS+ PT V  D  L ++ S RVV EVVSLGGGK+D L+DGG   LD 
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKEDGLNDGGDGALDT 120

Query: 121 RVQDDGGTARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGNSKYVAVGDE 180
           ++Q DGG  RAVSVTKYSPFWSERFHFLSAVKL+SDAT INVLPLRDFEG+SKYVAVGDE
Sbjct: 121 KIQ-DGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSKYVAVGDE 180

Query: 181 RGRVYVFMRNGDVAVELRTVSDSPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN 240
            GR+YVF+RNGDVAVELRT+S SPIT  LSYMSIYKNETLLVTGH++GAI M RIWEGSN
Sbjct: 181 TGRIYVFVRNGDVAVELRTMSGSPITATLSYMSIYKNETLLVTGHESGAISMLRIWEGSN 240

Query: 241 GEELNSLFMEYVVEFVATNGREEESPISILEVHHVGRTRYILSSDVRGKIKVFRENGAVY 300
           GEE NS+FME+V+EFV T+GREE+SPISILEVHHVGRTRYILSSDV+GKIKVFRE+G VY
Sbjct: 241 GEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDGTVY 300

Query: 301 GSVVPMSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDTMERSK 360
           GSV+P SRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFD  ERSK
Sbjct: 301 GSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSK 360

Query: 361 AYGFTSDGDLINVMLLGDIINFKCRVRSKRKFELDEPLVFQALKGYLLVINNKKVHIFNV 420
           AYGFTSDGDLI+VMLLGDI+NFKCRVRSK+KFELDEPL FQA+KGYLLVI ++KVH+FNV
Sbjct: 361 AYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSEKVHVFNV 420

Query: 421 SSQHYVRVGAPRHLFSVGLTEIRSSFMNYQNFDPKIEGKFIPLISSDHEKLIVLGLGGGY 480
           SSQHYVRVGAPR LFS  L EIRSSF+N QN D + +GKFIPLISSD EKL+VLGLGGGY
Sbjct: 421 SSQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVVLGLGGGY 480

Query: 481 VGMYRSNLSIFKGEFNTMLWTSPVLLFVLFLFGAWQFFAKKKEALTSWGPDDPFTATKPT 540
           +GMYRSNL I+KGEFNTMLWTSPVL F+LFLFGAWQFFAKKKEALTSWGPDDPFTAT PT
Sbjct: 481 IGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPT 540

Query: 541 TGAPLGIGSRE--------------RASFIDNSSRSTDMMDLRGGSGLRGPPRRYGSPTG 600
           TGAPLG GS E              R SFID  SRSTDMMDLRGGSGLRGPPRRYGSPTG
Sbjct: 541 TGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTG 600

Query: 601 YTGGATSSFRPATTNAHSSRPDAVDPNYRAASELKFRSSPLEPPGFPKRREPLFASNQVV 644
           Y GG TSSFRPATT+ HSSR  AVDPNYRAASELKFR SPLEPPGFPKRREPLFA+NQVV
Sbjct: 601 YPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVV 660

BLAST of Sed0021548 vs. ExPASy TrEMBL
Match: A0A6J1GHT9 (uncharacterized membrane protein At1g75140-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454317 PE=4 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 2.1e-301
Identity = 538/651 (82.64%), Postives = 579/651 (88.94%), Query Frame = 0

Query: 1   MANPLKGKLLLISFLF------VSRVFLCTSSSFEQNESESEVPFEIRQYDLLKNLEELV 60
           MANP KGKLL I FLF      VSRVF+C SSS E N+ ES+VP EIRQ+ LLKNLEELV
Sbjct: 1   MANPRKGKLLFIYFLFVFAIHRVSRVFVCNSSSLELNQPESDVPIEIRQHVLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDFPTPVVTDKNLKSEPSRVVAEVVSLGGGK-KDSLHDGGLDNRVQ 120
           +NLSDVVSKLE+RLSD PT + T             EV SLGGGK      DGGLDN +Q
Sbjct: 61  KNLSDVVSKLEMRLSDIPTKLNT-------------EVGSLGGGKGLSDGGDGGLDNGIQ 120

Query: 121 DDGGTARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGNSKYVAVGDERGR 180
             GG  RAV+VTKYSPFWSE+FHFLSAVKLDSDATCINVLPLRDFEG+SKYVAVGDERGR
Sbjct: 121 YGGG-GRAVTVTKYSPFWSEKFHFLSAVKLDSDATCINVLPLRDFEGHSKYVAVGDERGR 180

Query: 181 VYVFMRNGDVAVELRTVSDSPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSNGEE 240
           VYVFMRNGDVAVELRTVSDSPIT+MLSYMS+YKNETLLVTGHKNGAILMHRIWE SNGE+
Sbjct: 181 VYVFMRNGDVAVELRTVSDSPITSMLSYMSVYKNETLLVTGHKNGAILMHRIWERSNGED 240

Query: 241 LNSLFMEYVVEFVATNGREEESPISILEVHHVGRTRYILSSDVRGKIKVFRENGAVYGSV 300
           LNS+FME+VVEFVA    E+ESPISILEVH+VGR RYILSS+VRGKIKVFRE+G VYGSV
Sbjct: 241 LNSVFMEHVVEFVA----EDESPISILEVHYVGRIRYILSSNVRGKIKVFREDGTVYGSV 300

Query: 301 VPMSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDTMERSKAYG 360
           +PMSRPI FLKQRLLFLTE+GAGSLDLRSMKLRESECEGLN+SLARNYVFD +ERSKAYG
Sbjct: 301 MPMSRPIVFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDAVERSKAYG 360

Query: 361 FTSDGDLINVMLLGDIINFKCRVRSKRKFELDEPLVFQALKGYLLVINNKKVHIFNVSSQ 420
           FTSDGDLI+V+LLGDI+NFKCRVRSKRKFELDEPL+FQA+KGYLLVI+N+KVHI+NVSSQ
Sbjct: 361 FTSDGDLIHVLLLGDILNFKCRVRSKRKFELDEPLIFQAIKGYLLVISNEKVHIYNVSSQ 420

Query: 421 HYVRVGAPRHLFSVGLTEIRSSFMNYQNFDPKIEGKFIPLISSDHEKLIVLGLGGGYVGM 480
           HYVRVGAPRHLFS GL EIRSSF+NYQN D +  GKFIPLISSDHEKL+VLGLGGGYVG+
Sbjct: 421 HYVRVGAPRHLFSAGLDEIRSSFLNYQNMDSESGGKFIPLISSDHEKLVVLGLGGGYVGL 480

Query: 481 YRSNLSIFKGEFNTMLWTSPVLLFVLFLFGAWQFFAKKKEALTSWGPDDPFTATKPTTGA 540
           YRSNL IFKGEFNT+LWTSPVL F+LFLFGAW FFAKKKEALTSWGPDDPFTAT PTTGA
Sbjct: 481 YRSNLPIFKGEFNTVLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPTTGA 540

Query: 541 PLGIGSRERASFIDNSSRSTDMMDLR-GGSGLRGPPRRYGSPTGYTGGATSSFRPATTNA 600
           PLG GS ERASFID  SRSTDMMDLR GG GLRGPPRRYGSPTGY GGATSSFRPATT+ 
Sbjct: 541 PLGTGSSERASFIDAPSRSTDMMDLRGGGGGLRGPPRRYGSPTGYPGGATSSFRPATTSD 600

Query: 601 HSSRPDAVDPNYRAASELKFRSSPLEPPGFPKRREPLFASNQVVNQVVDES 644
           H+SRP AVDPNYRAASELKFR SPLEPPGFPKRREPLFA+NQVVNQVVDES
Sbjct: 601 HNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDES 633

BLAST of Sed0021548 vs. ExPASy TrEMBL
Match: A0A0A0LNK9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361500 PE=4 SV=1)

HSP 1 Score: 1028.5 bits (2658), Expect = 1.2e-296
Identity = 525/653 (80.40%), Postives = 570/653 (87.29%), Query Frame = 0

Query: 1   MANPLKGKLLLISFLF------VSRVFLCTSSSFEQNESESEVPFEIRQYDLLKNLEELV 60
           MA   KG+   I FLF      VSRVF+C+SSS EQN+SE EVPFEI+QYDLLKNLEELV
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSSSLEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDFPTPVVTDKNLKSEPSRVVAEV-VSLGGGKKDSLH---DGGLDN 120
           RNLSDVVSKLELRLSD PT V  +K       RVV EV      GK++ L+   DGGLD+
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDAFRRVVPEVGKEKRKGKEEGLNDGGDGGLDH 120

Query: 121 RVQDDGGTARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGNSKYVAVGDE 180
           ++Q DGG  RAVSVTKYS FWSERFHFLSAVKL++DATCINVLPLRDFEG+SKYVAVGDE
Sbjct: 121 KIQ-DGGRTRAVSVTKYSSFWSERFHFLSAVKLEADATCINVLPLRDFEGHSKYVAVGDE 180

Query: 181 RGRVYVFMRNGDVAVELRTVSDSPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN 240
           RGR+YVF+ NGDVA+EL TV  SP+T MLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN
Sbjct: 181 RGRIYVFVINGDVAIELPTVPGSPVTAMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN 240

Query: 241 GEELNSLFMEYVVEFVATNGREEESPISILEVHHVGRTRYILSSDVRGKIKVFRENGAVY 300
           GE+LN +FME+VVEFVATN RE+ES IS+LE+HHVGRTRYILSSD  GKIKVFRE+G VY
Sbjct: 241 GEDLNLIFMEHVVEFVATNSREDESQISLLELHHVGRTRYILSSDFGGKIKVFREDGTVY 300

Query: 301 GSVVPMSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDTMERSK 360
           GSV+P SRPIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLN+SLARNYVFD MERSK
Sbjct: 301 GSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDAMERSK 360

Query: 361 AYGFTSDGDLINVMLLGDIINFKCRVRSKRKFELDEPLVFQALKGYLLVINNKKVHIFNV 420
           AYG TSDGDLI+V+LLGDI+NFKCRVRSKRKFELDEPL FQ +KGYLLV +N+KVH+FNV
Sbjct: 361 AYGVTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNV 420

Query: 421 SSQHYVRVGAPRHLFSVGLTEIRSSFMNYQNFDPKIEGKFIPLISSDHEKLIVLGLGGGY 480
           SSQHYVRVGAPR LFS GL EI+SSF+NYQN D   E  FIPLISSDHEKL+VLGLGGGY
Sbjct: 421 SSQHYVRVGAPRLLFSAGLDEIKSSFLNYQNSDLVSEANFIPLISSDHEKLVVLGLGGGY 480

Query: 481 VGMYRSNLSIFKGEFNTMLWTSPVLLFVLFLFGAWQFFAKKKEALTSWGPDDPFTATKPT 540
           VGMYRSNL IFKGEFNTM+WTSPVL F+LFLFGAW FFAKKKEALTSWGPDDPFTAT PT
Sbjct: 481 VGMYRSNLPIFKGEFNTMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPT 540

Query: 541 TGAPLGIGSRERASFIDNSSRSTDMMDLRGGSGLRGPPRRYGSPTGYTGGATSSFRPATT 600
           TGAP+G GS ERASFID  SRSTD+MDLR   GLRGPPRRYGSPTGY  GATSSFRPATT
Sbjct: 541 TGAPMGTGSSERASFIDTPSRSTDVMDLRSAGGLRGPPRRYGSPTGYPAGATSSFRPATT 600

Query: 601 NAHSSRPDAVDPNYRAASELKFRSSPLEPPGFPKRREPLFASNQVVNQVVDES 644
           N HSSRP AVDPNYRAASELKFR SPLEPPGFPKRREPLFA+NQVVNQVVD+S
Sbjct: 601 NDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDS 652

BLAST of Sed0021548 vs. TAIR 10
Match: AT1G75140.1 (unknown protein; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G19370.1); Has 51 Blast hits to 49 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink). )

HSP 1 Score: 625.5 bits (1612), Expect = 4.6e-179
Identity = 352/666 (52.85%), Postives = 454/666 (68.17%), Query Frame = 0

Query: 1   MANPLKGKLLLISFLFVSRVFLCTSSSF-EQNESESE-VPFE--------------IRQY 60
           MA+   GK     F FVS + L  S S+ +   SES+ +P+E               RQ 
Sbjct: 1   MADSQNGKSAFF-FFFVSLILLFLSPSYSDVTASESDPIPYENSDASPGVVTSSESDRQG 60

Query: 61  DLLKNLEELVRNLSDVVSKLELRLSDFPTPVVTDKNLKSEPSRVVAEVVSLGGGKKDSLH 120
             L  LEELVRNL+++V++L+ +LS+ P  V      K E +R             D + 
Sbjct: 61  VSLHRLEELVRNLTELVARLDAKLSETPFKV------KKEITR-------------DEIE 120

Query: 121 DGGLDNRVQDDGGTARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGNSKY 180
           +             A+A SVTKYSPFWSERF F SAVKLDS+ATCINVLP RD EG SKY
Sbjct: 121 E------------KAKAFSVTKYSPFWSERFEFTSAVKLDSEATCINVLPFRDHEGLSKY 180

Query: 181 VAVGDERGRVYVFMRNGDVAVELRTVSDSPITTMLSYMSIYKNETLLVTGHKNGAILMHR 240
            AVGD  GRV+VF+RNGDV VE  T  DSPIT M+SYMS+YKNE+ +VTGH++G IL+HR
Sbjct: 181 FAVGDSSGRVFVFLRNGDVLVEFFTTCDSPITAMVSYMSVYKNESFVVTGHQSGVILLHR 240

Query: 241 IWEGSNGEELNSLFMEYVVEFVATNGREEESPISILEVHHVGRTRYILSSDVRGKIKVFR 300
           + EGS GE+LNS  ME V +F   +G E+   +++LEVHHVGR RYIL++D+ GK+ VF 
Sbjct: 241 LREGSIGEDLNSAVMENVGKF---DGTEDGLQVTLLEVHHVGRVRYILATDLSGKLTVFT 300

Query: 301 ENGAVYGSVVPMSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFD 360
           EN  VYGSV P SRP+ FLKQRLLFLTETGAGSLDLRSMK+RESECEGLN+SLAR+YVFD
Sbjct: 301 ENRTVYGSVSPTSRPLVFLKQRLLFLTETGAGSLDLRSMKIRESECEGLNHSLARSYVFD 360

Query: 361 TMERSKAYGFTSDGDLINVMLLGDIINFKCRVRSKRKFELDEPLVFQALKGYLLVINNKK 420
             ER+KAYGFTS+G++I+V+LLGDI+NFKCRVRSK+K +++EP+  QA+KGYLL++N +K
Sbjct: 361 ASERAKAYGFTSEGEIIHVLLLGDIMNFKCRVRSKKKVQMEEPVALQAIKGYLLIVNQEK 420

Query: 421 VHIFNVSSQHYVRVGAPRHLFSVGLTEIRSSFMNY----QNFDPKIEGKFIPLISSDHEK 480
           V ++NVS+QHYVR   PR LF   L +IRS+F+++    +  D +   K  PLI+SD EK
Sbjct: 421 VFVYNVSTQHYVRTTGPRLLFPAALEDIRSTFLSHRESTKTTDHQKLEKVTPLIASDREK 480

Query: 481 LIVLGLGGGYVGMYRSNLSIFKGEFNTMLWTSPVLLFVLFLFGAWQFFAKKKEALTSWGP 540
           L+V+GLG GYV  Y+S L I K EFNTMLW+SPV  F+LFLFGAW FF+KKKE+LT+WGP
Sbjct: 481 LLVMGLGDGYVATYKSKLPISKAEFNTMLWSSPVFFFILFLFGAWHFFSKKKESLTAWGP 540

Query: 541 DDPFTATKPTTGAPLGIGSRERASFIDNSSRSTDMMDLRGGSGLRGPPRRYGSPTGY-TG 600
           DDPF++T  T  +     ++  ++F +++ R+ D MDLR         RRY SP+ Y  G
Sbjct: 541 DDPFSST--TMSSSSTTTAQNSSAFSESTRRNDDHMDLR---------RRYVSPSRYPPG 600

Query: 601 GATSSFRPATTNAHSSRPDAVDPNYR-AASELKFR-SSPLEPPGFPKRREPLFASNQVVN 644
            AT ++R   +N  SSR      NYR  A E+K+R  S L+  GF KRRE LF +N+ ++
Sbjct: 601 AATGAYRSVGSNDPSSRAPVETTNYRTTAQEMKYRGGSGLDSGGFGKRRESLFGNNKALD 617

BLAST of Sed0021548 vs. TAIR 10
Match: AT1G19370.1 (unknown protein; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G75140.1); Has 45 Blast hits to 43 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 44; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 613.2 bits (1580), Expect = 2.3e-175
Identity = 339/655 (51.76%), Postives = 440/655 (67.18%), Query Frame = 0

Query: 1   MANPLKGKLLLISF--------LFVSRVFLCTSSSFEQNESESEVPFE--IRQYDLLKNL 60
           MA+PL GK   I F        LF+S ++   S   E   ++  V      R+  LL NL
Sbjct: 1   MADPLNGKSFFICFSLLFSFTLLFISPLYATESPVIEDVSTDVAVSVSETNREAVLLHNL 60

Query: 61  EELVRNLSDVVSKLELRLSDFPTPVVTDKNLKSEPSRVVAEVVSLGGGKKDSLHDGGLDN 120
           EELV+NL+++V+ L+ +LS  P   + +KN                   + S+ D   D 
Sbjct: 61  EELVKNLTELVANLDAKLSATP---LKEKN-------------------EISVDD---DI 120

Query: 121 RVQDDGGTARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGNSKYVAVGDE 180
             + + G A+A SVTKYSPFWSERF F SAVKL+SDATCINVLP RDFEG+SKY A+GD 
Sbjct: 121 GEEKERGRAKAFSVTKYSPFWSERFQFTSAVKLNSDATCINVLPFRDFEGSSKYFAIGDS 180

Query: 181 RGRVYVFMRNGDVAVELRTVSDSPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN 240
           +GRVYVF+RNGDV +E  T  DSP+T M+SY S++KN + +VTGH+NGA+L+HRI EGSN
Sbjct: 181 KGRVYVFLRNGDVLIEFFTTVDSPVTAMVSYSSVFKNSSFVVTGHQNGAVLLHRIHEGSN 240

Query: 241 GEE--LNSLFMEYVVEFVATNGREEESPISILEVHHVGRTRYILSSDVRGKIKVFRENGA 300
           GE+   NS+ ME+V +F   +  +   P+++LEVHHVGR RYIL++D+ GK+ V  EN  
Sbjct: 241 GEDWNSNSVSMEHVGKFDVDDSAD---PVTLLEVHHVGRVRYILATDLSGKLTVLTENRT 300

Query: 301 VYGSVVPMSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDTMER 360
           VYGSV+P SRP+ FLKQRLLFLTE+GAGSLDLRSMK+RE+ECEGLN+SLAR YVFD  ER
Sbjct: 301 VYGSVIPSSRPLVFLKQRLLFLTESGAGSLDLRSMKIRETECEGLNHSLARTYVFDAAER 360

Query: 361 SKAYGFTSDGDLINVMLLGDIINFKCRVRSKRKFELDEPLVFQALKGYLLVINNKKVHIF 420
           SKAYGFTS+G++I+V+L GDI+NFKCRVRSK+KF+++EP+  Q++KGYLLVIN +KV  F
Sbjct: 361 SKAYGFTSEGEIIHVLLHGDIMNFKCRVRSKKKFQMEEPVALQSIKGYLLVINEEKVFAF 420

Query: 421 NVSSQHYVRVGAPRHLFSVGLTEIRSSFMNYQNFDPKIEG--KFIPLISSDHEKLIVLGL 480
           NVS+QHYVR   PR LFS GL EIRS+F++++    +     K  PLI+SD E L+V+GL
Sbjct: 421 NVSTQHYVRTAGPRLLFSAGLEEIRSAFLSHRESSSRTTTVVKTRPLIASDRENLLVIGL 480

Query: 481 GGGYVGMYRSNLSIFKGEFNTMLWTSPVLLFVLFLFGAWQFFAKKKEALTSWGPDDPFTA 540
             GY  +Y+S L   KG+FNTMLW+SPV  F+LFLFGAW FFAKKKE+LT+WGPDDPFT 
Sbjct: 481 ENGYFAVYKSKLPTLKGDFNTMLWSSPVFFFILFLFGAWHFFAKKKESLTAWGPDDPFTP 540

Query: 541 TKPTTGAPLGIGSRERASFIDNSSRSTDMMDLRGGSGLRGPPRRYGSPTGYTGGATSSFR 600
           T           S +  +F + S R+ D+MDLR         RRY         A  S+R
Sbjct: 541 TGAQN------SSAKEPTFTEPSRRNDDLMDLR---------RRY---------AGGSYR 600

Query: 601 PATTNAHSSRPDAVDPNYRAASE----LKFRSSPLEPPGFPKRREPLFASNQVVN 638
               N  SSR   VD NYR  ++     +   S L+  GF  RR+ LF +N+V++
Sbjct: 601 SVGANDPSSRA-PVDGNYRTTAQDHNNYRGGGSGLDSNGFGNRRDHLFGNNKVLD 602