Sed0021458 (gene) Chayote v1

Overview
NameSed0021458
Typegene
OrganismSechium edule (Chayote v1)
Descriptionaspartic proteinase PCS1-like
LocationLG11: 35739559 .. 35741225 (+)
RNA-Seq ExpressionSed0021458
SyntenySed0021458
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAATATATAGGTCATGCCATCTCCATTAGAAGTTTGAATCATGTCCGAATTTTAATTTTTGTTATCATAAATATAAGAGCACCAATTTAACAGTCCATTATATTCGTTAACACATAAAACTCATTTCCCTTTCTTCTCCGATTCCAAAACCATGCCCTTCTTCCTCGCTCTGCAACTTCTCATCTTTATCCTCTGTTCCAAACAGAGCCTCTGTTCTTCTGCAACTCCGGCCATGATTCTTCCCCTTAAAACCCAGATGGGTTTCAATTCAAATCCTTCCAACAAGCTCAGTTTCCACCATAACGTCACTTTGACCGTCTCCTTGACCGTCGGGTCGCCCCCCCAACCCGTCACCATGGTTCTCGACACCGGCAGTGAACTCTCATGGCTTCACTGCAAAAAATCCCCAAATTTAAACTCTGTTTTCAACCCGGTTTCTTCCTCTTCTTACTCGCCAATCCCCTGTTCTTCCCCTGTTTGCCGGACCCGAACCCGAGACCTTCCCAACCCGGTTACCTGCGACCCGAAAAATCTTTGCCACGTCTACGTCTCCTACGCCGACGCCTCGTCTCTCGAAGGCAACCTCGCATCCGACACGTTTCGAGTCGGGTCATCGGCGCAACCCGGAACGTTCTTCGGGTGTATGGATTCGGGTTTCAGTTCGAATTCGGAAGAGGATGCGAAAACCACTGGGTTAATGGGTATGAACAGAGGATCGCTTTCTTTTGTGACCCAATTGGGTTTGCCGAAATTTTCTTATTGCATTTCGGGTCGGGATTCGACCGGCGTTCTGCTTTTCGGCGGCGGCGCCGTGTCGTGGCTTGGGAATTTGACGTACACGCCGTTGGTTCAAATGTCGACGCCGTTGCCGTACTACGACCGGGTCGCGTACACGGTTCAACTGGACGGGATCCGGGTCGGGGACAAAATCCTGCCGCTCCCGAAATCGATTTTCGCGCCGGATCACACCGGGGCGGGTCAGACCATGGTGGATTCGGGTACCCAGTTCACGTTTCTTCTCGGCCCGGTTTACACGGCGTTGAAGAACGAGTTTCTGTCGCAAACGAAAGGGGTTTTGGTTGAATTGGATGACCCGAATTTCGTGTTTCAAGGGGCGATGGATTCGTGTTTTAGAATTCCGATGAACCGGGGTGTCTCGCCGCCATTGCCGGCGGTGAGCTTGATGTTTCGTGGGGCGGAGATGGTGGTTTCCGGCGAGCGGCTGATGTACAAAGTGGCCGGAATGGTGAAGGGGAATGATTGGGTGTATTGCTTGACGTTTGGGAATTCGGATCTGTTGGGAATCGAAGCGTTTGTGATTGGGCATCATCATCAACAAAACGTGTGGATGGAATTTGATTTGGTTAAATCAAGGGTTGGATTTGTTGAAACGAGGTGTGATTTGGCGGGTCAACGGCTCGGGTTGGGCCTTTAGAATGTGGGCCTCCAATTTTGGTCGTAAGCACTGTCCAATCCATGGCCCACCTTAAGCCCGTACTAAGGGAGGGTGGCCCATTGATCGGACAGGGACTCTCTTTTTTTTCCTTTTTTTTTTTTTTTCAAATTTAAAATTTTATTATTGCTCTGTGAGATTCTCTGAAGAAAATTGGAGTGTAGTGGGGAAATGTCAGTGTTTTTTTAAAATTGTGTTTGTGTTTGTTCGAAG

mRNA sequence

AGAATATATAGGTCATGCCATCTCCATTAGAAGTTTGAATCATGTCCGAATTTTAATTTTTGTTATCATAAATATAAGAGCACCAATTTAACAGTCCATTATATTCGTTAACACATAAAACTCATTTCCCTTTCTTCTCCGATTCCAAAACCATGCCCTTCTTCCTCGCTCTGCAACTTCTCATCTTTATCCTCTGTTCCAAACAGAGCCTCTGTTCTTCTGCAACTCCGGCCATGATTCTTCCCCTTAAAACCCAGATGGGTTTCAATTCAAATCCTTCCAACAAGCTCAGTTTCCACCATAACGTCACTTTGACCGTCTCCTTGACCGTCGGGTCGCCCCCCCAACCCGTCACCATGGTTCTCGACACCGGCAGTGAACTCTCATGGCTTCACTGCAAAAAATCCCCAAATTTAAACTCTGTTTTCAACCCGGTTTCTTCCTCTTCTTACTCGCCAATCCCCTGTTCTTCCCCTGTTTGCCGGACCCGAACCCGAGACCTTCCCAACCCGGTTACCTGCGACCCGAAAAATCTTTGCCACGTCTACGTCTCCTACGCCGACGCCTCGTCTCTCGAAGGCAACCTCGCATCCGACACGTTTCGAGTCGGGTCATCGGCGCAACCCGGAACGTTCTTCGGGTGTATGGATTCGGGTTTCAGTTCGAATTCGGAAGAGGATGCGAAAACCACTGGGTTAATGGGTATGAACAGAGGATCGCTTTCTTTTGTGACCCAATTGGGTTTGCCGAAATTTTCTTATTGCATTTCGGGTCGGGATTCGACCGGCGTTCTGCTTTTCGGCGGCGGCGCCGTGTCGTGGCTTGGGAATTTGACGTACACGCCGTTGGTTCAAATGTCGACGCCGTTGCCGTACTACGACCGGGTCGCGTACACGGTTCAACTGGACGGGATCCGGGTCGGGGACAAAATCCTGCCGCTCCCGAAATCGATTTTCGCGCCGGATCACACCGGGGCGGGTCAGACCATGGTGGATTCGGGTACCCAGTTCACGTTTCTTCTCGGCCCGGTTTACACGGCGTTGAAGAACGAGTTTCTGTCGCAAACGAAAGGGGTTTTGGTTGAATTGGATGACCCGAATTTCGTGTTTCAAGGGGCGATGGATTCGTGTTTTAGAATTCCGATGAACCGGGGTGTCTCGCCGCCATTGCCGGCGGTGAGCTTGATGTTTCGTGGGGCGGAGATGGTGGTTTCCGGCGAGCGGCTGATGTACAAAGTGGCCGGAATGGTGAAGGGGAATGATTGGGTGTATTGCTTGACGTTTGGGAATTCGGATCTGTTGGGAATCGAAGCGTTTGTGATTGGGCATCATCATCAACAAAACGTGTGGATGGAATTTGATTTGGTTAAATCAAGGGTTGGATTTGTTGAAACGAGGTGTGATTTGGCGGGTCAACGGCTCGGGTTGGGCCTTTAGAATGTGGGCCTCCAATTTTGGTCGTAAGCACTGTCCAATCCATGGCCCACCTTAAGCCCGTACTAAGGGAGGGTGGCCCATTGATCGGACAGGGACTCTCTTTTTTTTCCTTTTTTTTTTTTTTTCAAATTTAAAATTTTATTATTGCTCTGTGAGATTCTCTGAAGAAAATTGGAGTGTAGTGGGGAAATGTCAGTGTTTTTTTAAAATTGTGTTTGTGTTTGTTCGAAG

Coding sequence (CDS)

ATGCCCTTCTTCCTCGCTCTGCAACTTCTCATCTTTATCCTCTGTTCCAAACAGAGCCTCTGTTCTTCTGCAACTCCGGCCATGATTCTTCCCCTTAAAACCCAGATGGGTTTCAATTCAAATCCTTCCAACAAGCTCAGTTTCCACCATAACGTCACTTTGACCGTCTCCTTGACCGTCGGGTCGCCCCCCCAACCCGTCACCATGGTTCTCGACACCGGCAGTGAACTCTCATGGCTTCACTGCAAAAAATCCCCAAATTTAAACTCTGTTTTCAACCCGGTTTCTTCCTCTTCTTACTCGCCAATCCCCTGTTCTTCCCCTGTTTGCCGGACCCGAACCCGAGACCTTCCCAACCCGGTTACCTGCGACCCGAAAAATCTTTGCCACGTCTACGTCTCCTACGCCGACGCCTCGTCTCTCGAAGGCAACCTCGCATCCGACACGTTTCGAGTCGGGTCATCGGCGCAACCCGGAACGTTCTTCGGGTGTATGGATTCGGGTTTCAGTTCGAATTCGGAAGAGGATGCGAAAACCACTGGGTTAATGGGTATGAACAGAGGATCGCTTTCTTTTGTGACCCAATTGGGTTTGCCGAAATTTTCTTATTGCATTTCGGGTCGGGATTCGACCGGCGTTCTGCTTTTCGGCGGCGGCGCCGTGTCGTGGCTTGGGAATTTGACGTACACGCCGTTGGTTCAAATGTCGACGCCGTTGCCGTACTACGACCGGGTCGCGTACACGGTTCAACTGGACGGGATCCGGGTCGGGGACAAAATCCTGCCGCTCCCGAAATCGATTTTCGCGCCGGATCACACCGGGGCGGGTCAGACCATGGTGGATTCGGGTACCCAGTTCACGTTTCTTCTCGGCCCGGTTTACACGGCGTTGAAGAACGAGTTTCTGTCGCAAACGAAAGGGGTTTTGGTTGAATTGGATGACCCGAATTTCGTGTTTCAAGGGGCGATGGATTCGTGTTTTAGAATTCCGATGAACCGGGGTGTCTCGCCGCCATTGCCGGCGGTGAGCTTGATGTTTCGTGGGGCGGAGATGGTGGTTTCCGGCGAGCGGCTGATGTACAAAGTGGCCGGAATGGTGAAGGGGAATGATTGGGTGTATTGCTTGACGTTTGGGAATTCGGATCTGTTGGGAATCGAAGCGTTTGTGATTGGGCATCATCATCAACAAAACGTGTGGATGGAATTTGATTTGGTTAAATCAAGGGTTGGATTTGTTGAAACGAGGTGTGATTTGGCGGGTCAACGGCTCGGGTTGGGCCTTTAG

Protein sequence

MPFFLALQLLIFILCSKQSLCSSATPAMILPLKTQMGFNSNPSNKLSFHHNVTLTVSLTVGSPPQPVTMVLDTGSELSWLHCKKSPNLNSVFNPVSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKNLCHVYVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSTGVLLFGGGAVSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLSQTKGVLVELDDPNFVFQGAMDSCFRIPMNRGVSPPLPAVSLMFRGAEMVVSGERLMYKVAGMVKGNDWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLGL
Homology
BLAST of Sed0021458 vs. NCBI nr
Match: XP_022938661.1 (aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 765.0 bits (1974), Expect = 3.4e-217
Identity = 375/427 (87.82%), Postives = 395/427 (92.51%), Query Frame = 0

Query: 1   MPFFL-ALQLLIFILCSKQSLCSSATPAMILPLKTQMGFNSNPSNKLSFHHNVTLTVSLT 60
           M FFL  LQLLI  +  KQ LC SAT  M+LPLKTQMG  S PSNKLSFHHNVTLTVSLT
Sbjct: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60

Query: 61  VGSPPQPVTMVLDTGSELSWLHCKKSPNLNSVFNPVSSSSYSPIPCSSPVCRTRTRDLPN 120
           +GSPPQPVTMVLDTGSELSWLHCKK+PNLNSVFNP+SSSSYSP+PC+SPVCRTRTRDLPN
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPN 120

Query: 121 PVTCDPKNLCHVYVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180
           PVTCDPK LCHV+VSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT
Sbjct: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180

Query: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSTGVLLFGGGAVSWLGNLTYTPLVQMSTPL 240
           TGLMGMNRGSLSFVTQLGLPKFSYCISGRDS+GVLLFG  ++SWLGNLTYTPLVQMSTPL
Sbjct: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240

Query: 241 PYYDRVAYTVQLDGIRVGDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300
           PYYDRVAYTVQLDGIRVG+KIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN
Sbjct: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300

Query: 301 EFLSQTKGVLVELDDPNFVFQGAMDSCFRIPMNRGVSPPLPAVSLMFRGAEMVVSGERLM 360
           EF+ QTKG+LV L DPNFVFQGAMD C+R+P  +G  PPLP VSLMFRGAEMVV GE LM
Sbjct: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360

Query: 361 YKVAGMVKGNDWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           YKV GMV+G D V+CLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420

Query: 421 GQRLGLG 427
           G+RLGLG
Sbjct: 421 GERLGLG 427

BLAST of Sed0021458 vs. NCBI nr
Match: KAG7015977.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 763.8 bits (1971), Expect = 7.7e-217
Identity = 374/427 (87.59%), Postives = 394/427 (92.27%), Query Frame = 0

Query: 1   MPFFL-ALQLLIFILCSKQSLCSSATPAMILPLKTQMGFNSNPSNKLSFHHNVTLTVSLT 60
           MPFFL  LQLLI  +  KQ LC SAT  M+LPLKTQMG  S PSNKLSFHHNVTLTVSLT
Sbjct: 1   MPFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60

Query: 61  VGSPPQPVTMVLDTGSELSWLHCKKSPNLNSVFNPVSSSSYSPIPCSSPVCRTRTRDLPN 120
           +GSPPQPVTMVLDTGSELSWLHCKK+PNLNSVFNP+SSSSYSP+PC+SPVCR RTRDLPN
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPN 120

Query: 121 PVTCDPKNLCHVYVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180
           PVTCDPK LCHV+VSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT
Sbjct: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180

Query: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSTGVLLFGGGAVSWLGNLTYTPLVQMSTPL 240
           TGLMGMNRGSLSFVTQ GLPKFSYCISGRDS+GVLLFG  ++SWLGNLTYTPLVQMSTPL
Sbjct: 181 TGLMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240

Query: 241 PYYDRVAYTVQLDGIRVGDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300
           PYYDRVAYTVQLDGIRVG+KIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN
Sbjct: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300

Query: 301 EFLSQTKGVLVELDDPNFVFQGAMDSCFRIPMNRGVSPPLPAVSLMFRGAEMVVSGERLM 360
           EF+ QTKG+LV L DPNFVFQGAMD C+R+P  +G  PPLP VSLMFRGAEMVV GE LM
Sbjct: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360

Query: 361 YKVAGMVKGNDWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           YKV GMV+G D V+CLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420

Query: 421 GQRLGLG 427
           G+RLGLG
Sbjct: 421 GERLGLG 427

BLAST of Sed0021458 vs. NCBI nr
Match: XP_023549570.1 (aspartic proteinase PCS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 761.1 bits (1964), Expect = 5.0e-216
Identity = 374/427 (87.59%), Postives = 393/427 (92.04%), Query Frame = 0

Query: 1   MPFFL-ALQLLIFILCSKQSLCSSATPAMILPLKTQMGFNSNPSNKLSFHHNVTLTVSLT 60
           M FFL  LQLLI  +  KQ LC SAT  M+LPLKTQMG  S PSNKLSFHHNVTLTVSLT
Sbjct: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60

Query: 61  VGSPPQPVTMVLDTGSELSWLHCKKSPNLNSVFNPVSSSSYSPIPCSSPVCRTRTRDLPN 120
           +GSPPQPVTMVLDTGSELSWLHCKK+PNLNSVFNP+SSSSYSP+PC+SPVCRTRTRDLPN
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPN 120

Query: 121 PVTCDPKNLCHVYVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180
           PVTCDPK LCHV+VSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT
Sbjct: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180

Query: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSTGVLLFGGGAVSWLGNLTYTPLVQMSTPL 240
           TGLMGMNRGSLSFVTQLGLPKFSYCISGRDS+GVLLFG  ++SWLGNLTYTPLVQMSTPL
Sbjct: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDVSLSWLGNLTYTPLVQMSTPL 240

Query: 241 PYYDRVAYTVQLDGIRVGDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300
           PYYDRVAYTVQLDGIRVG+KIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN
Sbjct: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300

Query: 301 EFLSQTKGVLVELDDPNFVFQGAMDSCFRIPMNRGVSPPLPAVSLMFRGAEMVVSGERLM 360
           EFL QTKG+LV L DPNFVFQGAMD C+ +P  +G  PPLP VSLMFRGAEMVV GE LM
Sbjct: 301 EFLVQTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360

Query: 361 YKVAGMVKGNDWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           YKV GMV+G D V+CLTFGNSDLLGIEAFVIGHHHQQNVWMEFDL KSRVGFVETRCDLA
Sbjct: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDLA 420

Query: 421 GQRLGLG 427
           G+RLGLG
Sbjct: 421 GERLGLG 427

BLAST of Sed0021458 vs. NCBI nr
Match: XP_022992613.1 (aspartic proteinase PCS1-like [Cucurbita maxima])

HSP 1 Score: 760.0 bits (1961), Expect = 1.1e-215
Identity = 372/427 (87.12%), Postives = 394/427 (92.27%), Query Frame = 0

Query: 1   MPFFL-ALQLLIFILCSKQSLCSSATPAMILPLKTQMGFNSNPSNKLSFHHNVTLTVSLT 60
           M FFL  L LLI  +  KQSLC SA   M+LPLKTQMG  S PSNKLSFHHNVTLTVSLT
Sbjct: 1   MAFFLRLLHLLICCVSFKQSLCFSAIQTMVLPLKTQMGVTSQPSNKLSFHHNVTLTVSLT 60

Query: 61  VGSPPQPVTMVLDTGSELSWLHCKKSPNLNSVFNPVSSSSYSPIPCSSPVCRTRTRDLPN 120
           +GSPPQPVTMVLDTGSELSWLHCKK+PNLNSVFNP+SSSSYSP+PC+SPVCRTRTRDLPN
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPN 120

Query: 121 PVTCDPKNLCHVYVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180
           PVTCDPK LCHV+VSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT
Sbjct: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180

Query: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSTGVLLFGGGAVSWLGNLTYTPLVQMSTPL 240
           TGLMGMNRGSLSFVTQLGLPKFSYCISGRDS+GVLLFG  ++SWLGNLTYTPLVQMSTPL
Sbjct: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240

Query: 241 PYYDRVAYTVQLDGIRVGDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300
           PYYDRVAYTVQLDGIRVG+KIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN
Sbjct: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300

Query: 301 EFLSQTKGVLVELDDPNFVFQGAMDSCFRIPMNRGVSPPLPAVSLMFRGAEMVVSGERLM 360
           EF+ QTKG+LV L DPNFVFQGAMD C+R+P  +G  PPLP VSLMFRGAEMVV GE LM
Sbjct: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360

Query: 361 YKVAGMVKGNDWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           Y+V GMV+G D V+C+TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 361 YRVPGMVRGGDQVHCVTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420

Query: 421 GQRLGLG 427
           G+RLGLG
Sbjct: 421 GERLGLG 427

BLAST of Sed0021458 vs. NCBI nr
Match: XP_038883963.1 (aspartic proteinase PCS1-like [Benincasa hispida])

HSP 1 Score: 754.2 bits (1946), Expect = 6.1e-214
Identity = 374/428 (87.38%), Postives = 391/428 (91.36%), Query Frame = 0

Query: 3   FFL--ALQLLIFILCSKQSLCSSATP-AMILPLKTQMGFNSNPSNKLSFHHNVTLTVSLT 62
           FFL   LQLLI+ L  KQSLC SAT   ++LPLKTQMG  S PSNKLSFHHNVTLTVSLT
Sbjct: 4   FFLLRLLQLLIYGLSLKQSLCFSATQMTLVLPLKTQMGLISQPSNKLSFHHNVTLTVSLT 63

Query: 63  VGSPPQPVTMVLDTGSELSWLHCKKSPNLNSVFNPVSSSSYSPIPCSSPVCRTRTRDLPN 122
           VGSPPQ VTMVLDTGSELSWLHCKKSPNL SVFNP+SSSSYSPIPCSSP+CRTRTRDLPN
Sbjct: 64  VGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDLPN 123

Query: 123 PVTCDPKNLCHVYVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 182
           PV CDPK LCHV+VSYADASSLEGNLASDTFR+GSSAQPGT FGCMDSGFSSNSEEDAKT
Sbjct: 124 PVICDPKKLCHVFVSYADASSLEGNLASDTFRIGSSAQPGTLFGCMDSGFSSNSEEDAKT 183

Query: 183 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSTGVLLFGGGAVSWLGNLTYTPLVQMSTPL 242
           TGLMGMNRGSLSFVTQLGLPKFSYCISG DS+GVLLFG   VSWLGNLTYTPLVQ+STPL
Sbjct: 184 TGLMGMNRGSLSFVTQLGLPKFSYCISGSDSSGVLLFGDAPVSWLGNLTYTPLVQISTPL 243

Query: 243 PYYDRVAYTVQLDGIRVGDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 302
           PYYDRVAYTVQLDGIRVG+KILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN
Sbjct: 244 PYYDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 303

Query: 303 EFLSQTKGVLVELDDPNFVFQGAMDSCFRIPMNRGVSPPLPAVSLMFRGAEMVVSGERLM 362
           EFL QTKGVL+ L DPNFVFQGAMD C+R+    G  PPLPAVSLMF GAEMVV GE L+
Sbjct: 304 EFLEQTKGVLIPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFHGAEMVVGGEVLL 363

Query: 363 YKVAGMVKGNDWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 422
           Y+V GM+KGN+ VYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 364 YRVPGMMKGNELVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 423

Query: 423 GQRLGLGL 428
           GQRLGLGL
Sbjct: 424 GQRLGLGL 431

BLAST of Sed0021458 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 3.2e-149
Identity = 256/413 (61.99%), Postives = 317/413 (76.76%), Query Frame = 0

Query: 22  SSATPAMILPLKTQM-GFNSNPSNKLSFHHNVTLTVSLTVGSPPQPVTMVLDTGSELSWL 81
           SS++  ++LPLKT++   +  P++KL FHHNVTLTV+LTVG+PPQ ++MV+DTGSELSWL
Sbjct: 40  SSSSQTLVLPLKTRITPTDHRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSELSWL 99

Query: 82  HCKKSPNLNSV--FNPVSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKNLCHVYVSYADA 141
            C +S N N V  F+P  SSSYSPIPCSSP CRTRTRD   P +CD   LCH  +SYADA
Sbjct: 100 RCNRSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLSYADA 159

Query: 142 SSLEGNLASDTFRVGSSAQPGT-FFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQLG 201
           SS EGNLA++ F  G+S       FGCM S   S+ EED KTTGL+GMNRGSLSF++Q+G
Sbjct: 160 SSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFISQMG 219

Query: 202 LPKFSYCISGRDS-TGVLLFGGGAVSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRV 261
            PKFSYCISG D   G LL G    +WL  L YTPL+++STPLPY+DRVAYTVQL GI+V
Sbjct: 220 FPKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTGIKV 279

Query: 262 GDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLSQTKGVLVELDDPN 321
             K+LP+PKS+  PDHTGAGQTMVDSGTQFTFLLGPVYTAL++ FL++T G+L   +DP+
Sbjct: 280 NGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPD 339

Query: 322 FVFQGAMDSCFRIP---MNRGVSPPLPAVSLMFRGAEMVVSGERLMYKVAGMVKGNDWVY 381
           FVFQG MD C+RI    +  G+   LP VSL+F GAE+ VSG+ L+Y+V  +  GND VY
Sbjct: 340 FVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTVGNDSVY 399

Query: 382 CLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLG 427
           C TFGNSDL+G+EA+VIGHHHQQN+W+EFDL +SR+G     CD++GQRLG+G
Sbjct: 400 CFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

BLAST of Sed0021458 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.9e-40
Identity = 115/368 (31.25%), Postives = 182/368 (49.46%), Query Frame = 0

Query: 56  VSLTVGSPPQPVTMVLDTGSELSWLHCKKSPNL----NSVFNPVSSSSYSPIPCSSPVCR 115
           ++L++G+P QP + ++DTGS+L W  C+           +FNP  SSS+S +PCSS +C+
Sbjct: 97  MNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQ 156

Query: 116 TRTRDLPNPVTCDPKNLCHVYVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSS 175
                L +P TC   N C     Y D S  +G++ ++T   GS + P   FGC   G ++
Sbjct: 157 A----LSSP-TCS-NNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGC---GENN 216

Query: 176 NSEEDAKTTGLMGMNRGSLSFVTQLGLPKFSYCIS--GRDSTGVLLFGGGAVSWLGNLTY 235
                    GL+GM RG LS  +QL + KFSYC++  G  +   LL G  A S       
Sbjct: 217 QGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPN 276

Query: 236 TPLVQMSTPLPYYDRVAYTVQLDGIRVGDKILPLPKSIFA-PDHTGAGQTMVDSGTQFTF 295
           T L+Q S+ +P +    Y + L+G+ VG   LP+  S FA   + G G  ++DSGT  T+
Sbjct: 277 TTLIQ-SSQIPTF----YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTY 336

Query: 296 LLGPVYTALKNEFLSQTKGVLVELDDPNFVFQGAMDSCFRIPMNRGVSPPLPAVSLMFRG 355
            +   Y +++ EF+SQ    +V      F      D CF+ P +   +  +P   + F G
Sbjct: 337 FVNNAYQSVRQEFISQINLPVVNGSSSGF------DLCFQTPSDPS-NLQIPTFVMHFDG 396

Query: 356 AEMVVSGERLMYKVAGMVKGNDWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSR 415
            ++ +  E         +  ++ + CL  G+S   G+  F  G+  QQN+ + +D   S 
Sbjct: 397 GDLELPSENY------FISPSNGLICLAMGSSS-QGMSIF--GNIQQQNMLVVYDTGNSV 434

Query: 416 VGFVETRC 417
           V F   +C
Sbjct: 457 VSFASAQC 434

BLAST of Sed0021458 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 5.4e-40
Identity = 116/367 (31.61%), Postives = 175/367 (47.68%), Query Frame = 0

Query: 56  VSLTVGSPPQPVTMVLDTGSELSWLHCKKSPNLNS----VFNPVSSSSYSPIPCSSPVCR 115
           +++ +G+P    + ++DTGS+L W  C+      S    +FNP  SSS+S +PC S  C 
Sbjct: 98  MNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYC- 157

Query: 116 TRTRDLPNPVTCDPKNLCHVYVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSS 175
              +DLP+  TC+  N C     Y D S+ +G +A++TF   +S+ P   FGC   G  +
Sbjct: 158 ---QDLPSE-TCN-NNECQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGC---GEDN 217

Query: 176 NSEEDAKTTGLMGMNRGSLSFVTQLGLPKFSYCIS--GRDSTGVLLFGGGAVSWLGNLTY 235
                    GL+GM  G LS  +QLG+ +FSYC++  G  S   L  G  A         
Sbjct: 218 QGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPS 277

Query: 236 TPLVQMSTPLPYYDRVAYTVQLDGIRVGDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFL 295
           T L+  S    Y     Y + L GI VG   L +P S F     G G  ++DSGT  T+L
Sbjct: 278 TTLIHSSLNPTY-----YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYL 337

Query: 296 LGPVYTALKNEFLSQTKGVLVELDDPNFVFQGAMDSCFRIPMNRGVSPPLPAVSLMFRGA 355
               Y A+   F  Q    L  +D+ +      + +CF+ P + G +  +P +S+ F G 
Sbjct: 338 PQDAYNAVAQAFTDQIN--LPTVDESS----SGLSTCFQQPSD-GSTVQVPEISMQFDGG 397

Query: 356 EMVVSGERLMYKVAGMVKGNDWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRV 415
            + +  + ++   A      + V CL  G+S  LGI  F  G+  QQ   + +DL    V
Sbjct: 398 VLNLGEQNILISPA------EGVICLAMGSSSQLGISIF--GNIQQQETQVLYDLQNLAV 435

Query: 416 GFVETRC 417
            FV T+C
Sbjct: 458 SFVPTQC 435

BLAST of Sed0021458 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 8.4e-33
Identity = 125/391 (31.97%), Postives = 180/391 (46.04%), Query Frame = 0

Query: 37  GFNSNPSNKLSFHHNVTLTVSLTVGSPPQPVTMVLDTGSELSWLHCKKSPNLNS----VF 96
           GF+S+  + LS       T  L VG+P + V MVLDTGS++ WL C       S    +F
Sbjct: 126 GFSSSVVSGLSQGSGEYFT-RLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIF 185

Query: 97  NPVSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKNLCHVYVSYADASSLEGNLASDTFRV 156
           +P  S +Y+ IPCSSP CR     L +      +  C   VSY D S   G+ +++T   
Sbjct: 186 DPRKSKTYATIPCSSPHCRR----LDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTF 245

Query: 157 GSSAQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQLG---LPKFSYCISGRD 216
             +   G   GC       N        GL+G+ +G LSF  Q G     KFSYC+  R 
Sbjct: 246 RRNRVKGVALGC----GHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRS 305

Query: 217 ST---GVLLFGGGAVSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGDKILP-LPK 276
           ++     ++FG  AVS +    +TPL+      P  D   Y V L GI VG   +P +  
Sbjct: 306 ASSKPSSVVFGNAAVSRIAR--FTPLLSN----PKLD-TFYYVGLLGISVGGTRVPGVTA 365

Query: 277 SIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLSQTKGVLVELDDPNFVFQGAMDS 336
           S+F  D  G G  ++DSGT  T L+ P Y A+++ F     G       P+F      D+
Sbjct: 366 SLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAF---RVGAKTLKRAPDF---SLFDT 425

Query: 337 CFRIPMNRGVSPPLPAVSLMFRGAEMVVSGERLMYKVAGMVKGNDWVYCLTFGNSDLLGI 396
           CF +     V   +P V L FRGA++ +     +  V       +  +C  F  + + G+
Sbjct: 426 CFDLSNMNEVK--VPTVVLHFRGADVSLPATNYLIPV-----DTNGKFCFAFAGT-MGGL 484

Query: 397 EAFVIGHHHQQNVWMEFDLVKSRVGFVETRC 417
              +IG+  QQ   + +DL  SRVGF    C
Sbjct: 486 S--IIGNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of Sed0021458 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.0e-30
Identity = 118/377 (31.30%), Postives = 178/377 (47.21%), Query Frame = 0

Query: 60  VGSPPQPVTMVLDTGSELSWLHCKKSPNL--NSVFNPVSSSSYSPIPCSSPVCRT-RTRD 119
           +GSP Q + + LDT ++ +W HC        +S+F P +SSSY+ +PCSS  C   + + 
Sbjct: 85  LGSPSQQLLLALDTSADATWAHCSPCGTCPSSSLFAPANSSSYASLPCSSSWCPLFQGQA 144

Query: 120 LPNP-----VTCDPKNL--CHVYVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGF 179
            P P         P  L  C     +ADA S +  LASDT R+G  A P   FGC+ S  
Sbjct: 145 CPAPQGGGDAAPPPATLPTCAFSKPFADA-SFQAALASDTLRLGKDAIPNYTFGCVSS-- 204

Query: 180 SSNSEEDAKTTGLMGMNRGSLSFVTQLGL---PKFSYCISGRDS---TGVLLFGGGAVSW 239
            +    +    GL+G+ RG ++ ++Q G      FSYC+    S   +G L  G G    
Sbjct: 205 VTGPTTNMPRQGLLGLGRGPMALLSQAGSLYNGVFSYCLPSYRSYYFSGSLRLGAGG-GQ 264

Query: 240 LGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGDKILPLPKSIFAPD-HTGAGQTMVDS 299
             ++ YTP+++     P+   + Y V + G+ VG   + +P   FA D  TGAG T+VDS
Sbjct: 265 PRSVRYTPMLRN----PHRSSL-YYVNVTGLSVGHAWVKVPAGSFAFDAATGAG-TVVDS 324

Query: 300 GTQFTFLLGPVYTALKNEFLSQTKGVLVELDDPNFVFQGAMDSCFRIPMNRGVSPPLPAV 359
           GT  T    PVY AL+ EF  Q            +   GA D+CF    +   +   PAV
Sbjct: 325 GTVITRWTAPVYAALREEFRRQVAA------PSGYTSLGAFDTCFN--TDEVAAGGAPAV 384

Query: 360 SL-MFRGAEMVVSGERLMYKVAGMVKGNDWVYCLTFGNS-DLLGIEAFVIGHHHQQNVWM 418
           ++ M  G ++ +  E  +   +        + CL    +   +     VI +  QQN+ +
Sbjct: 385 TVHMDGGVDLALPMENTLIHSSATP-----LACLAMAEAPQNVNSVVNVIANLQQQNIRV 438

BLAST of Sed0021458 vs. ExPASy TrEMBL
Match: A0A6J1FDS6 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111444820 PE=3 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 1.7e-217
Identity = 375/427 (87.82%), Postives = 395/427 (92.51%), Query Frame = 0

Query: 1   MPFFL-ALQLLIFILCSKQSLCSSATPAMILPLKTQMGFNSNPSNKLSFHHNVTLTVSLT 60
           M FFL  LQLLI  +  KQ LC SAT  M+LPLKTQMG  S PSNKLSFHHNVTLTVSLT
Sbjct: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60

Query: 61  VGSPPQPVTMVLDTGSELSWLHCKKSPNLNSVFNPVSSSSYSPIPCSSPVCRTRTRDLPN 120
           +GSPPQPVTMVLDTGSELSWLHCKK+PNLNSVFNP+SSSSYSP+PC+SPVCRTRTRDLPN
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPN 120

Query: 121 PVTCDPKNLCHVYVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180
           PVTCDPK LCHV+VSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT
Sbjct: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180

Query: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSTGVLLFGGGAVSWLGNLTYTPLVQMSTPL 240
           TGLMGMNRGSLSFVTQLGLPKFSYCISGRDS+GVLLFG  ++SWLGNLTYTPLVQMSTPL
Sbjct: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240

Query: 241 PYYDRVAYTVQLDGIRVGDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300
           PYYDRVAYTVQLDGIRVG+KIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN
Sbjct: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300

Query: 301 EFLSQTKGVLVELDDPNFVFQGAMDSCFRIPMNRGVSPPLPAVSLMFRGAEMVVSGERLM 360
           EF+ QTKG+LV L DPNFVFQGAMD C+R+P  +G  PPLP VSLMFRGAEMVV GE LM
Sbjct: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360

Query: 361 YKVAGMVKGNDWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           YKV GMV+G D V+CLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420

Query: 421 GQRLGLG 427
           G+RLGLG
Sbjct: 421 GERLGLG 427

BLAST of Sed0021458 vs. ExPASy TrEMBL
Match: A0A6J1JZR1 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111488906 PE=3 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 5.4e-216
Identity = 372/427 (87.12%), Postives = 394/427 (92.27%), Query Frame = 0

Query: 1   MPFFL-ALQLLIFILCSKQSLCSSATPAMILPLKTQMGFNSNPSNKLSFHHNVTLTVSLT 60
           M FFL  L LLI  +  KQSLC SA   M+LPLKTQMG  S PSNKLSFHHNVTLTVSLT
Sbjct: 1   MAFFLRLLHLLICCVSFKQSLCFSAIQTMVLPLKTQMGVTSQPSNKLSFHHNVTLTVSLT 60

Query: 61  VGSPPQPVTMVLDTGSELSWLHCKKSPNLNSVFNPVSSSSYSPIPCSSPVCRTRTRDLPN 120
           +GSPPQPVTMVLDTGSELSWLHCKK+PNLNSVFNP+SSSSYSP+PC+SPVCRTRTRDLPN
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPN 120

Query: 121 PVTCDPKNLCHVYVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180
           PVTCDPK LCHV+VSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT
Sbjct: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180

Query: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSTGVLLFGGGAVSWLGNLTYTPLVQMSTPL 240
           TGLMGMNRGSLSFVTQLGLPKFSYCISGRDS+GVLLFG  ++SWLGNLTYTPLVQMSTPL
Sbjct: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240

Query: 241 PYYDRVAYTVQLDGIRVGDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300
           PYYDRVAYTVQLDGIRVG+KIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN
Sbjct: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300

Query: 301 EFLSQTKGVLVELDDPNFVFQGAMDSCFRIPMNRGVSPPLPAVSLMFRGAEMVVSGERLM 360
           EF+ QTKG+LV L DPNFVFQGAMD C+R+P  +G  PPLP VSLMFRGAEMVV GE LM
Sbjct: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360

Query: 361 YKVAGMVKGNDWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           Y+V GMV+G D V+C+TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 361 YRVPGMVRGGDQVHCVTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420

Query: 421 GQRLGLG 427
           G+RLGLG
Sbjct: 421 GERLGLG 427

BLAST of Sed0021458 vs. ExPASy TrEMBL
Match: A0A1S3B3Y8 (aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103485512 PE=3 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 2.8e-212
Identity = 370/428 (86.45%), Postives = 386/428 (90.19%), Query Frame = 0

Query: 1   MPFFLALQLLIFILCSKQSLCSSATP-AMILPLKTQMGFNSNPSNKLSFHHNVTLTVSLT 60
           M FF     L+  L  KQSLC SATP  M+LPL+TQMG  S PSNKLSFHHNVTLTVSLT
Sbjct: 1   MSFFFFFFFLVVSLSMKQSLCFSATPTTMVLPLQTQMGLISQPSNKLSFHHNVTLTVSLT 60

Query: 61  VGSPPQPVTMVLDTGSELSWLHCKKSPNLNSVFNPVSSSSYSPIPCSSPVCRTRTRDLPN 120
           VGSPPQ VTMVLDTGSELSWLHCKKSPNL SVFNP+SSSSYSPIPCSSPVCRTRTRDLPN
Sbjct: 61  VGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPVCRTRTRDLPN 120

Query: 121 PVTCDPKNLCHVYVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180
           PVTCDPK LCH  VSYADASSLEGNLASD FR+GSSA PGT FGCMDSGFSSNSEEDAKT
Sbjct: 121 PVTCDPKKLCHAIVSYADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDAKT 180

Query: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSTGVLLFGGGAVSWLGNLTYTPLVQMSTPL 240
           TGLMGMNRGSLSFVTQLGLPKFSYCISGRDS+GVLLFG   +SWLGNLTYTPLVQ+STPL
Sbjct: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDSHLSWLGNLTYTPLVQISTPL 240

Query: 241 PYYDRVAYTVQLDGIRVGDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300
           PY+DRVAYTVQLDGIRVG+KILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTAL+N
Sbjct: 241 PYFDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALRN 300

Query: 301 EFLSQTKGVLVELDDPNFVFQGAMDSCFRIPMNRGVSPPLPAVSLMFRGAEMVVSGERLM 360
           EFL QTKGVL  L DPNFVFQGAMD C+R+P   G  P LPAVSLMFRGAEMVV GE L+
Sbjct: 301 EFLEQTKGVLAPLGDPNFVFQGAMDLCYRVPAG-GKLPELPAVSLMFRGAEMVVGGEVLL 360

Query: 361 YKVAGMVKGNDWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           YKV GM+KG +WVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 361 YKVPGMMKGKEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420

Query: 421 GQRLGLGL 428
           GQRLGLGL
Sbjct: 421 GQRLGLGL 427

BLAST of Sed0021458 vs. ExPASy TrEMBL
Match: A0A5D3DLY6 (Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G00670 PE=3 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 2.3e-211
Identity = 365/412 (88.59%), Postives = 380/412 (92.23%), Query Frame = 0

Query: 17  KQSLCSSATP-AMILPLKTQMGFNSNPSNKLSFHHNVTLTVSLTVGSPPQPVTMVLDTGS 76
           KQSLC SATP  M+LPL+TQMG  S PSNKLSFHHNVTLTVSLTVGSPPQ VTMVLDTGS
Sbjct: 2   KQSLCFSATPTTMVLPLQTQMGLISQPSNKLSFHHNVTLTVSLTVGSPPQQVTMVLDTGS 61

Query: 77  ELSWLHCKKSPNLNSVFNPVSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKNLCHVYVSY 136
           ELSWLHCKKSPNL SVFNP+SSSSYSPIPCSSPVCRTRTRDLPNPVTCDPK LCH  VSY
Sbjct: 62  ELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKKLCHAIVSY 121

Query: 137 ADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ 196
           ADASSLEGNLASD FR+GSSA PGT FGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ
Sbjct: 122 ADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ 181

Query: 197 LGLPKFSYCISGRDSTGVLLFGGGAVSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIR 256
           LGLPKFSYCISGRDS+GVLLFG   +SWLGNLTYTPLVQ+STPLPY+DRVAYTVQLDGIR
Sbjct: 182 LGLPKFSYCISGRDSSGVLLFGDSHLSWLGNLTYTPLVQISTPLPYFDRVAYTVQLDGIR 241

Query: 257 VGDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLSQTKGVLVELDDP 316
           VG+KILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTAL+NEFL QTKGVL  L DP
Sbjct: 242 VGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALRNEFLEQTKGVLAPLGDP 301

Query: 317 NFVFQGAMDSCFRIPMNRGVSPPLPAVSLMFRGAEMVVSGERLMYKVAGMVKGNDWVYCL 376
           NFVFQGAMD C+R+P   G  P LPAVSLMFRGAEMVV GE L+YKV GM+KG +WVYCL
Sbjct: 302 NFVFQGAMDLCYRVPAG-GKLPELPAVSLMFRGAEMVVGGEVLLYKVPGMMKGKEWVYCL 361

Query: 377 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLGL 428
           TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLGL
Sbjct: 362 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLGL 412

BLAST of Sed0021458 vs. ExPASy TrEMBL
Match: E5GC71 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 2.3e-211
Identity = 365/412 (88.59%), Postives = 380/412 (92.23%), Query Frame = 0

Query: 17  KQSLCSSATP-AMILPLKTQMGFNSNPSNKLSFHHNVTLTVSLTVGSPPQPVTMVLDTGS 76
           KQSLC SATP  M+LPL+TQMG  S PSNKLSFHHNVTLTVSLTVGSPPQ VTMVLDTGS
Sbjct: 2   KQSLCFSATPTTMVLPLQTQMGLISQPSNKLSFHHNVTLTVSLTVGSPPQQVTMVLDTGS 61

Query: 77  ELSWLHCKKSPNLNSVFNPVSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKNLCHVYVSY 136
           ELSWLHCKKSPNL SVFNP+SSSSYSPIPCSSPVCRTRTRDLPNPVTCDPK LCH  VSY
Sbjct: 62  ELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKKLCHAIVSY 121

Query: 137 ADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ 196
           ADASSLEGNLASD FR+GSSA PGT FGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ
Sbjct: 122 ADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ 181

Query: 197 LGLPKFSYCISGRDSTGVLLFGGGAVSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIR 256
           LGLPKFSYCISGRDS+GVLLFG   +SWLGNLTYTPLVQ+STPLPY+DRVAYTVQLDGIR
Sbjct: 182 LGLPKFSYCISGRDSSGVLLFGDSHLSWLGNLTYTPLVQISTPLPYFDRVAYTVQLDGIR 241

Query: 257 VGDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLSQTKGVLVELDDP 316
           VG+KILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTAL+NEFL QTKGVL  L DP
Sbjct: 242 VGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALRNEFLEQTKGVLAPLGDP 301

Query: 317 NFVFQGAMDSCFRIPMNRGVSPPLPAVSLMFRGAEMVVSGERLMYKVAGMVKGNDWVYCL 376
           NFVFQGAMD C+R+P   G  P LPAVSLMFRGAEMVV GE L+YKV GM+KG +WVYCL
Sbjct: 302 NFVFQGAMDLCYRVPAG-GKLPELPAVSLMFRGAEMVVGGEVLLYKVPGMMKGKEWVYCL 361

Query: 377 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLGL 428
           TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLGL
Sbjct: 362 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLGL 412

BLAST of Sed0021458 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 599.4 bits (1544), Expect = 2.3e-171
Identity = 301/426 (70.66%), Postives = 343/426 (80.52%), Query Frame = 0

Query: 4   FLALQLLIFILCSKQSLCSSATPAMILPLKTQMGFNSNPSNKLSFHHNVTLTVSLTVGSP 63
           FL + +L+ I        SS    ++  LKTQ     + S+KLSF HNVTLTV+L VG P
Sbjct: 16  FLRISVLLLIFPLTFCKTSSTNQTLLFSLKTQK-LPQSSSDKLSFRHNVTLTVTLAVGDP 75

Query: 64  PQPVTMVLDTGSELSWLHCKKSPNLNSVFNPVSSSSYSPIPCSSPVCRTRTRDLPNPVTC 123
           PQ ++MVLDTGSELSWLHCKKSPNL SVFNPVSSS+YSP+PCSSP+CRTRTRDLP P +C
Sbjct: 76  PQNISMVLDTGSELSWLHCKKSPNLGSVFNPVSSSTYSPVPCSSPICRTRTRDLPIPASC 135

Query: 124 DPK-NLCHVYVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGL 183
           DPK +LCHV +SYADA+S+EGNLA +TF +GS  +PGT FGCMDSG SSNSEEDAK+TGL
Sbjct: 136 DPKTHLCHVAISYADATSIEGNLAHETFVIGSVTRPGTLFGCMDSGLSSNSEEDAKSTGL 195

Query: 184 MGMNRGSLSFVTQLGLPKFSYCISGRDSTGVLLFGGGAVSWLGNLTYTPLVQMSTPLPYY 243
           MGMNRGSLSFV QLG  KFSYCISG DS+G LL G  + SWLG + YTPLV  STPLPY+
Sbjct: 196 MGMNRGSLSFVNQLGFSKFSYCISGSDSSGFLLLGDASYSWLGPIQYTPLVLQSTPLPYF 255

Query: 244 DRVAYTVQLDGIRVGDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFL 303
           DRVAYTVQL+GIRVG KIL LPKS+F PDHTGAGQTMVDSGTQFTFL+GPVYTALKNEF+
Sbjct: 256 DRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQTMVDSGTQFTFLMGPVYTALKNEFI 315

Query: 304 SQTKGVLVELDDPNFVFQGAMDSCFRI-PMNRGVSPPLPAVSLMFRGAEMVVSGERLMYK 363
           +QTK VL  +DDP+FVFQG MD C+++    R     LP VSLMFRGAEM VSG++L+Y+
Sbjct: 316 TQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNFSGLPMVSLMFRGAEMSVSGQKLLYR 375

Query: 364 VAGM-VKGNDWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFV-ETRCDLA 423
           V G   +G + VYC TFGNSDLLGIEAFVIGHHHQQNVWMEFDL KSRVGF    RCDLA
Sbjct: 376 VNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFAGNVRCDLA 435

Query: 424 GQRLGL 426
            QRLGL
Sbjct: 436 SQRLGL 440

BLAST of Sed0021458 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 529.6 bits (1363), Expect = 2.3e-150
Identity = 256/413 (61.99%), Postives = 317/413 (76.76%), Query Frame = 0

Query: 22  SSATPAMILPLKTQM-GFNSNPSNKLSFHHNVTLTVSLTVGSPPQPVTMVLDTGSELSWL 81
           SS++  ++LPLKT++   +  P++KL FHHNVTLTV+LTVG+PPQ ++MV+DTGSELSWL
Sbjct: 40  SSSSQTLVLPLKTRITPTDHRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSELSWL 99

Query: 82  HCKKSPNLNSV--FNPVSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKNLCHVYVSYADA 141
            C +S N N V  F+P  SSSYSPIPCSSP CRTRTRD   P +CD   LCH  +SYADA
Sbjct: 100 RCNRSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLSYADA 159

Query: 142 SSLEGNLASDTFRVGSSAQPGT-FFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQLG 201
           SS EGNLA++ F  G+S       FGCM S   S+ EED KTTGL+GMNRGSLSF++Q+G
Sbjct: 160 SSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFISQMG 219

Query: 202 LPKFSYCISGRDS-TGVLLFGGGAVSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRV 261
            PKFSYCISG D   G LL G    +WL  L YTPL+++STPLPY+DRVAYTVQL GI+V
Sbjct: 220 FPKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTGIKV 279

Query: 262 GDKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLSQTKGVLVELDDPN 321
             K+LP+PKS+  PDHTGAGQTMVDSGTQFTFLLGPVYTAL++ FL++T G+L   +DP+
Sbjct: 280 NGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPD 339

Query: 322 FVFQGAMDSCFRIP---MNRGVSPPLPAVSLMFRGAEMVVSGERLMYKVAGMVKGNDWVY 381
           FVFQG MD C+RI    +  G+   LP VSL+F GAE+ VSG+ L+Y+V  +  GND VY
Sbjct: 340 FVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTVGNDSVY 399

Query: 382 CLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLG 427
           C TFGNSDL+G+EA+VIGHHHQQN+W+EFDL +SR+G     CD++GQRLG+G
Sbjct: 400 CFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

BLAST of Sed0021458 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 242.7 bits (618), Expect = 5.5e-64
Identity = 159/454 (35.02%), Postives = 225/454 (49.56%), Query Frame = 0

Query: 2   PFFLALQLLIFILCSKQSLCSSATPAMILPL--------------KTQMGFNSN---PSN 61
           P FL L  + F  C   SL  S++ ++  PL              KT +    N   PS+
Sbjct: 7   PLFLKLLYIFFFFCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPPSS 66

Query: 62  KLSFHHNV----TLTVSLTVGSPPQPVTMVLDTGSELSWLHC------KKSPNLNSVFNP 121
             +F  N+     L +SL +G+P Q   +VLDTGS+LSW+ C      K  P   + F+P
Sbjct: 67  PYTFRSNIKYSMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPPPTTSFDP 126

Query: 122 VSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKNLCHVYVSYADASSLEGNLASDTFRVGS 181
             SSS+S +PCS P+C+ R  D   P +CD   LCH    YAD +  EGNL  + F   +
Sbjct: 127 SLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSN 186

Query: 182 S-AQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQLGLPKFSYCISGRD---- 241
           S   P    GC        ++E     G++GMN G LSF++Q  + KFSYCI  R     
Sbjct: 187 SQTTPPLILGC--------AKESTDEKGILGMNLGRLSFISQAKISKFSYCIPTRSNRPG 246

Query: 242 --STGVLLFGGGAVSWLGNLTYTPLV--QMSTPLPYYDRVAYTVQLDGIRVGDKILPLPK 301
             STG    G    S      Y  L+    S  +P  D +AYTV L GIR+G K L +P 
Sbjct: 247 LASTGSFYLGDNPNS--RGFKYVSLLTFPQSQRMPNLDPLAYTVPLQGIRIGQKRLNIPG 306

Query: 302 SIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLSQTKGVLVELDDPNFVFQGAMDS 361
           S+F PD  G+GQTMVDSG++FT L+   Y  +K E +      L +     +V+    D 
Sbjct: 307 SVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIVRLVGSRLKK----GYVYGSTADM 366

Query: 362 CFRIPMNRGVSPPLPAVSLMF-RGAEMVVSGERLMYKVAGMVKGNDWVYCLTFGNSDLLG 419
           CF    +  +   +  +   F RG E++V  + L+  V G       ++C+  G S +LG
Sbjct: 367 CFDGNHSMEIGRLIGDLVFEFGRGVEILVEKQSLLVNVGG------GIHCVGIGRSSMLG 426

BLAST of Sed0021458 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 238.8 bits (608), Expect = 8.0e-63
Identity = 147/393 (37.40%), Postives = 209/393 (53.18%), Query Frame = 0

Query: 39  NSNPSN-KLSFHHNVTLTVSLTVGSPPQPVTMVLDTGSELSWLHC---KKSPNLNSVFNP 98
           +S P N +  F +++ L +SL +G+PPQ   MVLDTGS+LSW+ C   K  P   + F+P
Sbjct: 56  SSPPYNFRSRFKYSMALIISLPIGTPPQAQQMVLDTGSQLSWIQCHRKKLPPKPKTSFDP 115

Query: 99  VSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKNLCHVYVSYADASSLEGNLASDTFRVGS 158
             SSS+S +PCS P+C+ R  D   P +CD   LCH    YAD +  EGNL  +     +
Sbjct: 116 SLSSSFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSN 175

Query: 159 S-AQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQLGLPKFSYCI------SG 218
           +   P    GC        + E +   G++GMNRG LSFV+Q  + KFSYCI       G
Sbjct: 176 TEITPPLILGC--------ATESSDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPG 235

Query: 219 RDSTGVLLFGGGAVSWLGNLTYTPLVQM--STPLPYYDRVAYTVQLDGIRVGDKILPLPK 278
              TG    G    S      Y  L+    S  +P  D +AYTV + GIR G K L +  
Sbjct: 236 FTPTGSFYLGDNPNS--HGFKYVSLLTFPESQRMPNLDPLAYTVPMIGIRFGLKKLNISG 295

Query: 279 SIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLSQTKGVLVELDDPNFVFQGAMDS 338
           S+F PD  G+GQTMVDSG++FT L+   Y  ++ E +++    L +     +V+ G  D 
Sbjct: 296 SVFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIMTRVGRRLKK----GYVYGGTADM 355

Query: 339 CFRIPMNRGVSPPL--PAVSLMFRGAEMVVSGERLMYKVAGMVKGNDWVYCLTFGNSDLL 398
           CF    N  + P L    V +  RG E++V  ER++  V G       ++C+  G S +L
Sbjct: 356 CF--DGNVAMIPRLIGDLVFVFTRGVEILVPKERVLVNVGG------GIHCVGIGRSSML 415

Query: 399 GIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRC 417
           G  + +IG+ HQQN+W+EFD+   RVGF +  C
Sbjct: 416 GAASNIIGNVHQQNLWVEFDVTNRRVGFAKADC 426

BLAST of Sed0021458 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 161.0 bits (406), Expect = 2.1e-39
Identity = 121/373 (32.44%), Postives = 185/373 (49.60%), Query Frame = 0

Query: 56  VSLTVGSPPQPVTMVLDTGSELSWLHCKKSPNL----NSVFNPVSSSSYSPIPCSSPVCR 115
           + L++G+P    + ++DTGS+L W  CK           +F+P  SSSYS + CSS +C 
Sbjct: 109 MELSIGNPAVKYSAIVDTGSDLIWTQCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCN 168

Query: 116 TRTRDLPNPVTCDPKNLCHVYVSYADASSLEGNLASDTFRV-GSSAQPGTFFGCMDSGFS 175
                LP     + K+ C    +Y D SS  G LA++TF     ++  G  FGC   G  
Sbjct: 169 A----LPRSNCNEDKDACEYLYTYGDYSSTRGLLATETFTFEDENSISGIGFGC---GVE 228

Query: 176 SNSEEDAKTTGLMGMNRGSLSFVTQLGLPKFSYCISGRDST--GVLLFGGGAVSWLGNLT 235
           +  +  ++ +GL+G+ RG LS ++QL   KFSYC++  + +     LF G   S + N T
Sbjct: 229 NEGDGFSQGSGLVGLGRGPLSLISQLKETKFSYCLTSIEDSEASSSLFIGSLASGIVNKT 288

Query: 236 YTPL----VQMSTPLPYYDRVA-YTVQLDGIRVGDKILPLPKSIFAPDHTGAGQTMVDSG 295
              L     +  + L   D+ + Y ++L GI VG K L + KS F     G G  ++DSG
Sbjct: 289 GASLDGEVTKTMSLLRNPDQPSFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDSG 348

Query: 296 TQFTFLLGPVYTALKNEFLSQTKGVLVELDDPNFVFQGAMDSCFRIPMNRGVSPPLPAVS 355
           T  T+L    +  LK EF S+   + + +DD        +D CF++P +   +  +P + 
Sbjct: 349 TTITYLEETAFKVLKEEFTSR---MSLPVDDSG---STGLDLCFKLP-DAAKNIAVPKMI 408

Query: 356 LMFRGAEMVVSGERLMYKVAGMVKGNDWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFD 415
             F+GA++ + GE   Y VA    G   V CL  G+S+ + I     G+  QQN  +  D
Sbjct: 409 FHFKGADLELPGEN--YMVADSSTG---VLCLAMGSSNGMSI----FGNVQQQNFNVLHD 458

Query: 416 LVKSRVGFVETRC 417
           L K  V FV T C
Sbjct: 469 LEKETVSFVPTEC 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022938661.13.4e-21787.82aspartic proteinase PCS1-like [Cucurbita moschata][more]
KAG7015977.17.7e-21787.59Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023549570.15.0e-21687.59aspartic proteinase PCS1-like [Cucurbita pepo subsp. pepo][more]
XP_022992613.11.1e-21587.12aspartic proteinase PCS1-like [Cucurbita maxima][more]
XP_038883963.16.1e-21487.38aspartic proteinase PCS1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9LZL33.2e-14961.99Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1[more]
Q766C31.9e-4031.25Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C25.4e-4031.61Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9LNJ38.4e-3331.97Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q6F4N51.0e-3031.30Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FDS61.7e-21787.82aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111444820 PE=3... [more]
A0A6J1JZR15.4e-21687.12aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111488906 PE=3 S... [more]
A0A1S3B3Y82.8e-21286.45aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103485512 PE=3 SV=1[more]
A0A5D3DLY62.3e-21188.59Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
E5GC712.3e-21188.59Aspartic proteinase nepenthesin-1 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT2G39710.12.3e-17170.66Eukaryotic aspartyl protease family protein [more]
AT5G02190.12.3e-15061.99Eukaryotic aspartyl protease family protein [more]
AT5G37540.15.5e-6435.02Eukaryotic aspartyl protease family protein [more]
AT1G66180.18.0e-6337.40Eukaryotic aspartyl protease family protein [more]
AT2G03200.12.1e-3932.44Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 212..225
score: 30.35
coord: 60..80
score: 43.68
coord: 278..289
score: 32.91
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 3..425
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 246..412
e-value: 3.5E-37
score: 127.7
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 47..217
e-value: 2.0E-37
score: 131.0
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 222..419
e-value: 1.8E-43
score: 150.3
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 54..416
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 56..218
e-value: 9.7E-40
score: 136.7
NoneNo IPR availablePANTHERPTHR47965:SF49EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 3..425
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 69..80
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 54..412
score: 37.050949
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 53..416
e-value: 2.16854E-72
score: 226.375

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0021458.1Sed0021458.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity