Sed0021386 (gene) Chayote v1

BLAST of Sed0021386 vs. NCBI nr
Match: XP_008448713.1 (PREDICTED: beta-amyrin 11-oxidase-like isoform X2 [Cucumis melo])

HSP 1 Score: 623.2 bits (1606), Expect = 1.7e-174
Identity = 302/420 (71.90%), Postives = 363/420 (86.43%), Query Frame = 0

Query: 1   MELEKLLVLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYA 60
           MEL++ LVL IG   +++GV+KRLNGW+Y AKLG  W KLPPGDM WP LG TLSF+K  
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  STGRLPNFLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQA 120
           ++G+ P  L      R+GK++MYKTHLLG A+IIV TSE+CRQVL DEE F PSLP+S  
Sbjct: 61  NSGQ-PRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSIT 120

Query: 121 MLTGRKSLFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIE 180
           +L+GRKSL  V KAEHRRLRRLT AP+SGH ALEMYI+HIEQTVI+ LEEW+SMK +PIE
Sbjct: 121 LLSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMK-KPIE 180

Query: 181 LLTEMKKLTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKAR 240
           LLTE+KKLTFKIIWNIFMGSSSID + GE+EALFAQ+A GF+S PINFPGF FH++LKAR
Sbjct: 181 LLTEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKAR 240

Query: 241 KKMHEVLQSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFA 300
           K++  +LQSI+NQKR+ KK+EG+NWEAKD MD+L+E+++EDGE LDDETIRDLIFGKLFA
Sbjct: 241 KELLSLLQSIINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFA 300

Query: 301 GHETSAFTAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVID 360
           GHETSA+TAMWAV+FLTNHP++ Q+ KEEQE+I+++RPTTQKGL+LSE+KQMK+LYQVID
Sbjct: 301 GHETSAYTAMWAVLFLTNHPHILQRTKEEQEDIVKRRPTTQKGLSLSEVKQMKFLYQVID 360

Query: 361 ETLRVSYITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWDV 420
           ETLRV  IT M+FREA+ DVEINGK+IP+GWKV+ WFGELY DEK FPSP EFNPSRWD+
Sbjct: 361 ETLRVGSITFMVFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKQFPSPHEFNPSRWDL 418

BLAST of Sed0021386 vs. NCBI nr
Match: XP_008448712.1 (PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 622.9 bits (1605), Expect = 2.2e-174
Identity = 302/419 (72.08%), Postives = 362/419 (86.40%), Query Frame = 0

Query: 1   MELEKLLVLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYA 60
           MEL++ LVL IG   +++GV+KRLNGW+Y AKLG  W KLPPGDM WP LG TLSF+K  
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  STGRLPNFLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQA 120
           ++G+ P  L      R+GK++MYKTHLLG A+IIV TSE+CRQVL DEE F PSLP+S  
Sbjct: 61  NSGQ-PRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSIT 120

Query: 121 MLTGRKSLFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIE 180
           +L+GRKSL  V KAEHRRLRRLT AP+SGH ALEMYI+HIEQTVI+ LEEW+SMK +PIE
Sbjct: 121 LLSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMK-KPIE 180

Query: 181 LLTEMKKLTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKAR 240
           LLTE+KKLTFKIIWNIFMGSSSID + GE+EALFAQ+A GF+S PINFPGF FH++LKAR
Sbjct: 181 LLTEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKAR 240

Query: 241 KKMHEVLQSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFA 300
           K++  +LQSI+NQKR+ KK+EG+NWEAKD MD+L+E+++EDGE LDDETIRDLIFGKLFA
Sbjct: 241 KELLSLLQSIINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFA 300

Query: 301 GHETSAFTAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVID 360
           GHETSA+TAMWAV+FLTNHP++ Q+ KEEQE+I+++RPTTQKGL+LSE+KQMK+LYQVID
Sbjct: 301 GHETSAYTAMWAVLFLTNHPHILQRTKEEQEDIVKRRPTTQKGLSLSEVKQMKFLYQVID 360

Query: 361 ETLRVSYITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWD 420
           ETLRV  IT M+FREA+ DVEINGK+IP+GWKV+ WFGELY DEK FPSP EFNPSRWD
Sbjct: 361 ETLRVGSITFMVFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKQFPSPHEFNPSRWD 417

BLAST of Sed0021386 vs. NCBI nr
Match: XP_022923278.1 (beta-amyrin 11-oxidase-like isoform X1 [Cucurbita moschata] >KAG6577626.1 Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 617.8 bits (1592), Expect = 7.2e-173
Identity = 297/419 (70.88%), Postives = 359/419 (85.68%), Query Frame = 0

Query: 1   MELEKLLVLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYA 60
           M ++  LVL +  FV V G+LKR+NGW+YAAKLG  W+ LPPGDM WP LG T+S++K  
Sbjct: 4   MGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYIKSF 63

Query: 61  STGRLPNFLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQA 120
            +G+  NF+  F++ R+GK +MYKTH+LG ATIIV +SELCRQVLGDE+ F PSLPK+  
Sbjct: 64  ISGQPSNFIANFYI-RYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNIN 123

Query: 121 MLTGRKSLFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIE 180
           MLTGRKSL  V KAEH+RLRRLT AP+SGH ALEMYI+HIE TV++ LEEW+SMKN PIE
Sbjct: 124 MLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKN-PIE 183

Query: 181 LLTEMKKLTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKAR 240
           +L+E+KKLTFKIIWNIFMGSSS++ + GE+E LF++VA GF+S PINFPGF  H+ALKAR
Sbjct: 184 VLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKAR 243

Query: 241 KKMHEVLQSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFA 300
           KK+  +LQSI+NQKRLV++R+G +WEAKD MD+LMEV++EDGE LDDETIRDLIFGKLFA
Sbjct: 244 KKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFA 303

Query: 301 GHETSAFTAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVID 360
           GHETSA+TAMWAV+FLT HP++FQ+AKEEQE I+ QRPTTQKG+ LSE+KQMK+LYQVID
Sbjct: 304 GHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVID 363

Query: 361 ETLRVSYITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWD 420
           ETLRV  +T MLFREA+ DVEINGK+IP+GWKV+ WFGELY DEK FPSPQEFNPSRWD
Sbjct: 364 ETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420

BLAST of Sed0021386 vs. NCBI nr
Match: XP_023552542.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 617.1 bits (1590), Expect = 1.2e-172
Identity = 297/419 (70.88%), Postives = 360/419 (85.92%), Query Frame = 0

Query: 1   MELEKLLVLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYA 60
           ME++  LVL +  FV V G+LKR+NGW+YAAKLG  W KLPPG M WP LG T+S++K  
Sbjct: 4   MEVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWPKLPPGHMGWPLLGSTISYIKNF 63

Query: 61  STGRLPNFLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQA 120
            +G+  NF+  F++ R+GK++MYKTH+LG ATIIV +SELCRQVLGDE+ F PSLPK+  
Sbjct: 64  ISGQPSNFMANFYI-RYGKSDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNIN 123

Query: 121 MLTGRKSLFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIE 180
           MLTGRKSL  V KAEH+RLRRLT AP+SGH ALEMYI+HIE TV++ LEEW+SMKN PIE
Sbjct: 124 MLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKN-PIE 183

Query: 181 LLTEMKKLTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKAR 240
           LL+E+KKLTFKIIWNIFMGSSS++ + GE+E LF++VA GF+S PINFPGF  H+ALKAR
Sbjct: 184 LLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKAR 243

Query: 241 KKMHEVLQSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFA 300
           KK+ ++LQSI+NQKRLV++R+G +WEAKD MD+LMEV++EDGE LDDETIRDLIFGKLFA
Sbjct: 244 KKLLDILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFA 303

Query: 301 GHETSAFTAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVID 360
           GHETSA+TAMWAV+FLT HP++FQ+AKEEQ+ II QRPTTQKG+ LSE+KQMK+L Q+ID
Sbjct: 304 GHETSAYTAMWAVLFLTKHPHIFQRAKEEQDRIITQRPTTQKGIILSEVKQMKFLSQIID 363

Query: 361 ETLRVSYITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWD 420
           ETLRV  +T MLFREA+ DVEINGK+IP+GWKV+ WFGELY DEK FPSPQEFNPSRWD
Sbjct: 364 ETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420

BLAST of Sed0021386 vs. NCBI nr
Match: XP_023007796.1 (beta-amyrin 11-oxidase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 617.1 bits (1590), Expect = 1.2e-172
Identity = 298/419 (71.12%), Postives = 360/419 (85.92%), Query Frame = 0

Query: 1   MELEKLLVLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYA 60
           ME++  LVL +  FV V G+LKRLNGW+YAAKLG  W KLPPGDM WP LG T+S++K  
Sbjct: 4   MEVKNWLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYIKNF 63

Query: 61  STGRLPNFLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQA 120
            +G+  NF+  F++ R+GK +MYKTHLLG ATIIV +SELCRQVLGDE+ F PSLPK+  
Sbjct: 64  ISGQPSNFIANFYI-RYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNIN 123

Query: 121 MLTGRKSLFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIE 180
           MLTGRKSL  V KAEH+RLRRLT AP+SGH ALEMYI+HIE TV++ LEEW+SMKN PIE
Sbjct: 124 MLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKN-PIE 183

Query: 181 LLTEMKKLTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKAR 240
           LL+E+KKLTFKIIWNIFMGSSS++ + GE+E LF++VA GF+S PINFPGF  H+ALKAR
Sbjct: 184 LLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKAR 243

Query: 241 KKMHEVLQSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFA 300
           KK+ ++LQSI++QKRLV+KR+G +WEAKD MD+LMEV++EDGE LDDETIRDLIFGKLFA
Sbjct: 244 KKLLDILQSIIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFA 303

Query: 301 GHETSAFTAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVID 360
           GHETSA+TAMWAV+FLT HP++FQ+AKEEQE I+ QRPTTQKG+ LSE+K+MK+L Q+ID
Sbjct: 304 GHETSAYTAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQIID 363

Query: 361 ETLRVSYITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWD 420
           ETLRV  +T MLFREA+ DVEINGK+IP+GWKV+ WFGELY DEK FPSP+EFNPSRWD
Sbjct: 364 ETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWD 420

BLAST of Sed0021386 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.4e-107
Identity = 196/408 (48.04%), Postives = 283/408 (69.36%), Query Frame = 0

Query: 13  CFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYASTGRLPNFLNTF 72
           C++     ++ LNGW+Y  KL  K   LPPGDM WP +G  LSF+K  S+G   +F+N  
Sbjct: 16  CYIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKDFSSGHPDSFINNL 75

Query: 73  FMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQAMLTGRKSLFTVT 132
            + ++G++ +YKTHL GN +IIV   ++CR+VL D+  F    PKS   L   + +  V+
Sbjct: 76  VL-KYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMIDVS 135

Query: 133 KAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIELLTEMKKLTFKI 192
            AEHR  RRL T+P+ GHKAL MY+  +E+ VIN LEE SSMK+ P+ELL EMKK++FK 
Sbjct: 136 NAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKH-PVELLKEMKKVSFKA 195

Query: 193 IWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKARKKMHEVLQSILN 252
           I ++FMGSS+ D    ++ + F  +  G  S PIN PGF FH+AL+ARKK+ +++Q +++
Sbjct: 196 IVHVFMGSSNQDI-IKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARKKLAKIVQPVVD 255

Query: 253 QKRL-VKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFAGHETSAFTAMW 312
           ++RL ++    +  + KD +D+L+EV++E+G  L+DE I DL+ G LFAGHE++A + MW
Sbjct: 256 ERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLFAGHESTATSLMW 315

Query: 313 AVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVIDETLRVSYITPM 372
           ++ +LT HP++ +KAKEEQE I R R ++QK L+L EIKQM YL QVIDETLR + I   
Sbjct: 316 SITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQVIDETLRCANIAFA 375

Query: 373 LFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWD 420
            FREA  DV ING +IP+GW+V+ W   ++ D +++P+P+EFNPSRWD
Sbjct: 376 TFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWD 420

BLAST of Sed0021386 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 7.1e-99
Identity = 189/416 (45.43%), Postives = 272/416 (65.38%), Query Frame = 0

Query: 8   VLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYASTGRLPN 67
           ++ +GCF + + +++++N W Y + LG     LPPGD+ WP +G  LSF++   T    +
Sbjct: 14  LMVLGCFGLNW-LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDS 73

Query: 68  FLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQAMLTGRKS 127
           F  T  + R+G   +YK H+ GN +IIV TS+ CR+VL D++ F P  P S   L GRKS
Sbjct: 74  FTRT-LIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKS 133

Query: 128 LFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIELLTEMKK 187
              ++  EH+RLRRLT APV+GH+AL  YI +IE+ VI  L++W+ M     E LT ++K
Sbjct: 134 FVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE--FEFLTHLRK 193

Query: 188 LTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKARKKMHEVL 247
           LTF+II  IF+ S S +     LE  +  +  G  +  +N PGF +HRALKARK +    
Sbjct: 194 LTFRIIMYIFLSSES-ENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAF 253

Query: 248 QSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFAGHETSAF 307
           QSI+ ++R  +K+   +   KD +D L+ V++EDG+ LDDE I D++   L AGHE+S  
Sbjct: 254 QSIVTERRNQRKQNILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGH 313

Query: 308 TAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVIDETLRVSY 367
           T MWA +FL  HP V Q+AK EQE I++ RP  QKGL+L E ++M++L QV+DETLRV  
Sbjct: 314 TIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVIT 373

Query: 368 ITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWDVSFI 424
            +   FREA TDVE+NG LIP+GWKV+TWF +++ D + FP P++F+P+RWD  F+
Sbjct: 374 FSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFV 423

BLAST of Sed0021386 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 351.3 bits (900), Expect = 1.6e-95
Identity = 188/412 (45.63%), Postives = 269/412 (65.29%), Query Frame = 0

Query: 8   VLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYASTGRLPN 67
           ++ +G FV+ + VLKR+N W Y +KLG K   LPPGD+ WP +G   SF++   T    +
Sbjct: 13  LIILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPES 72

Query: 68  FLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQAMLTGRKS 127
           F+ + ++ R+G+  +YK H+ G   ++V T E CR+VL D++ F    PKS   L GRKS
Sbjct: 73  FIQS-YITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 132

Query: 128 LFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIELLTEMKK 187
              ++  EH+RLRRLT+APV+G +AL +YI  IE+TV  DLE+WS M    IE L+ ++K
Sbjct: 133 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE--IEFLSHLRK 192

Query: 188 LTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKARKKMHEVL 247
           LTFK+I  IF+ S S +     LE  +  +  G  +  IN PGF +HRALKARKK+    
Sbjct: 193 LTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 252

Query: 248 QSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFAGHETSAF 307
           QSI+  +R  +K+   +   KD +D L++V++E+G  LDDE I DL+   L AGHE+S  
Sbjct: 253 QSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGH 312

Query: 308 TAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVIDETLRVSY 367
             MWA I +  HP + QKAKEEQE I+++R   QK LTL E ++M YL QVIDETLRV  
Sbjct: 313 LTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVIT 372

Query: 368 ITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWD 420
            +   FREA +DV+++G +IP+GWKV+TWF  ++ D + +P P++F+PSRW+
Sbjct: 373 FSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWE 417

BLAST of Sed0021386 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 5.1e-89
Identity = 170/406 (41.87%), Postives = 255/406 (62.81%), Query Frame = 0

Query: 15  VIVYGVLKRLNGWFYAAKLG-NKWAKLPPGDMSWPALGCTLSFVKYASTGRLPNFLNTFF 74
           V+V  V++R + W   A LG  + ++LPPG+M WP +G   +F++   +G  P+     F
Sbjct: 29  VLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKSGN-PDAFIASF 88

Query: 75  MARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQAMLTGRKSLFTVTK 134
           + R G+  +Y+T +  + TI+  T E C+QVL D+E F+   PK+   L G KS   ++ 
Sbjct: 89  IRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPKSFVNMSY 148

Query: 135 AEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIELLTEMKKLTFKII 194
            +HRR+R+LT AP++G  AL  Y+S I+QTV+  L  WSS ++  +E LTE++++TFKII
Sbjct: 149 DDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMTFKII 208

Query: 195 WNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKARKKMHEVLQSILNQ 254
             IFM S + D T   LE  +  +  G  +  IN PGF ++RAL+AR+K+  VLQ +L+ 
Sbjct: 209 VQIFM-SGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRKLVSVLQGVLDG 268

Query: 255 KRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFAGHETSAFTAMWAV 314
           +R    +  K   A D MD L+E  +E G  L D+ I D++   L AGHE+S    MWA 
Sbjct: 269 RRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGHESSGHITMWAT 328

Query: 315 IFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVIDETLRVSYITPMLF 374
           +FL  +P++F +AK EQE I+R  P TQ GLTL + K+M +L QV+DETLR   I+ + F
Sbjct: 329 VFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDETLRCVNISFVSF 388

Query: 375 REALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWD 420
           R+A  D+ +NG LIP+GWKV  W+  ++ D++ +P P+ FNPSRW+
Sbjct: 389 RQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWE 432

BLAST of Sed0021386 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.4e-83
Identity = 162/419 (38.66%), Postives = 263/419 (62.77%), Query Frame = 0

Query: 2   ELEKLLVLSIGCFVIVYGVLKRLNGWFYAAKLG-NKWAKLPPGDMSWPALGCTLSFVKYA 61
           E  + +++     V+V    +R +GW+  A LG  + A+LPPG+M WP +G   +F++  
Sbjct: 33  EAAEAVLVGAVALVVVDAAARRAHGWYREAALGAARRARLPPGEMGWPLVGGMWAFLRAF 92

Query: 62  STGRLPNFLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQA 121
            +G+ P+     F+ R G+  +Y++ +  + T++V T+E C+QVL D++ F+   PK+  
Sbjct: 93  KSGK-PDAFIASFVRRFGRTGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATV 152

Query: 122 MLTGRKSLFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIE 181
            L G +S   +   EHRR+R+LT AP++G  AL  Y+  I++TV + L  W+      +E
Sbjct: 153 ALVGPRSFVAMPYDEHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWAD-HGGSVE 212

Query: 182 LLTEMKKLTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKAR 241
            LTE++++TFKII  IF+G +    T   LE  + ++  G  +  IN PGF +  AL+AR
Sbjct: 213 FLTELRRMTFKIIVQIFLGGAD-QATTRALERSYTELNYGMRAMAINLPGFAYRGALRAR 272

Query: 242 KKMHEVLQSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFA 301
           +++  VLQ +L+++R  + + G +    D MD L+E ++E G  LDD+ I D++   L A
Sbjct: 273 RRLVAVLQGVLDERRAARAK-GVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNA 332

Query: 302 GHETSAFTAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVID 361
           GHE+S    MWA +FL  +P++F +AK EQE I+R  P++Q+GLTL + ++M+YL QVID
Sbjct: 333 GHESSGHITMWATVFLQENPDMFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVID 392

Query: 362 ETLRVSYITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWD 420
           ETLR+  I+ + FR+A  DV +NG LIP+GWKV  W+  ++ D + +P P +F+PSRW+
Sbjct: 393 ETLRLVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWE 447

BLAST of Sed0021386 vs. ExPASy TrEMBL
Match: A0A1S3BKD0 (beta-amyrin 11-oxidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490801 PE=4 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 8.3e-175
Identity = 302/420 (71.90%), Postives = 363/420 (86.43%), Query Frame = 0

Query: 1   MELEKLLVLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYA 60
           MEL++ LVL IG   +++GV+KRLNGW+Y AKLG  W KLPPGDM WP LG TLSF+K  
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  STGRLPNFLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQA 120
           ++G+ P  L      R+GK++MYKTHLLG A+IIV TSE+CRQVL DEE F PSLP+S  
Sbjct: 61  NSGQ-PRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSIT 120

Query: 121 MLTGRKSLFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIE 180
           +L+GRKSL  V KAEHRRLRRLT AP+SGH ALEMYI+HIEQTVI+ LEEW+SMK +PIE
Sbjct: 121 LLSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMK-KPIE 180

Query: 181 LLTEMKKLTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKAR 240
           LLTE+KKLTFKIIWNIFMGSSSID + GE+EALFAQ+A GF+S PINFPGF FH++LKAR
Sbjct: 181 LLTEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKAR 240

Query: 241 KKMHEVLQSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFA 300
           K++  +LQSI+NQKR+ KK+EG+NWEAKD MD+L+E+++EDGE LDDETIRDLIFGKLFA
Sbjct: 241 KELLSLLQSIINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFA 300

Query: 301 GHETSAFTAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVID 360
           GHETSA+TAMWAV+FLTNHP++ Q+ KEEQE+I+++RPTTQKGL+LSE+KQMK+LYQVID
Sbjct: 301 GHETSAYTAMWAVLFLTNHPHILQRTKEEQEDIVKRRPTTQKGLSLSEVKQMKFLYQVID 360

Query: 361 ETLRVSYITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWDV 420
           ETLRV  IT M+FREA+ DVEINGK+IP+GWKV+ WFGELY DEK FPSP EFNPSRWD+
Sbjct: 361 ETLRVGSITFMVFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKQFPSPHEFNPSRWDL 418

BLAST of Sed0021386 vs. ExPASy TrEMBL
Match: A0A1S3BJR1 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490801 PE=3 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 1.1e-174
Identity = 302/419 (72.08%), Postives = 362/419 (86.40%), Query Frame = 0

Query: 1   MELEKLLVLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYA 60
           MEL++ LVL IG   +++GV+KRLNGW+Y AKLG  W KLPPGDM WP LG TLSF+K  
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  STGRLPNFLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQA 120
           ++G+ P  L      R+GK++MYKTHLLG A+IIV TSE+CRQVL DEE F PSLP+S  
Sbjct: 61  NSGQ-PRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSIT 120

Query: 121 MLTGRKSLFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIE 180
           +L+GRKSL  V KAEHRRLRRLT AP+SGH ALEMYI+HIEQTVI+ LEEW+SMK +PIE
Sbjct: 121 LLSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMK-KPIE 180

Query: 181 LLTEMKKLTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKAR 240
           LLTE+KKLTFKIIWNIFMGSSSID + GE+EALFAQ+A GF+S PINFPGF FH++LKAR
Sbjct: 181 LLTEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKAR 240

Query: 241 KKMHEVLQSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFA 300
           K++  +LQSI+NQKR+ KK+EG+NWEAKD MD+L+E+++EDGE LDDETIRDLIFGKLFA
Sbjct: 241 KELLSLLQSIINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFA 300

Query: 301 GHETSAFTAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVID 360
           GHETSA+TAMWAV+FLTNHP++ Q+ KEEQE+I+++RPTTQKGL+LSE+KQMK+LYQVID
Sbjct: 301 GHETSAYTAMWAVLFLTNHPHILQRTKEEQEDIVKRRPTTQKGLSLSEVKQMKFLYQVID 360

Query: 361 ETLRVSYITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWD 420
           ETLRV  IT M+FREA+ DVEINGK+IP+GWKV+ WFGELY DEK FPSP EFNPSRWD
Sbjct: 361 ETLRVGSITFMVFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKQFPSPHEFNPSRWD 417

BLAST of Sed0021386 vs. ExPASy TrEMBL
Match: A0A6J1E938 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431020 PE=3 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 3.5e-173
Identity = 297/419 (70.88%), Postives = 359/419 (85.68%), Query Frame = 0

Query: 1   MELEKLLVLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYA 60
           M ++  LVL +  FV V G+LKR+NGW+YAAKLG  W+ LPPGDM WP LG T+S++K  
Sbjct: 4   MGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYIKSF 63

Query: 61  STGRLPNFLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQA 120
            +G+  NF+  F++ R+GK +MYKTH+LG ATIIV +SELCRQVLGDE+ F PSLPK+  
Sbjct: 64  ISGQPSNFIANFYI-RYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNIN 123

Query: 121 MLTGRKSLFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIE 180
           MLTGRKSL  V KAEH+RLRRLT AP+SGH ALEMYI+HIE TV++ LEEW+SMKN PIE
Sbjct: 124 MLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKN-PIE 183

Query: 181 LLTEMKKLTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKAR 240
           +L+E+KKLTFKIIWNIFMGSSS++ + GE+E LF++VA GF+S PINFPGF  H+ALKAR
Sbjct: 184 VLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKAR 243

Query: 241 KKMHEVLQSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFA 300
           KK+  +LQSI+NQKRLV++R+G +WEAKD MD+LMEV++EDGE LDDETIRDLIFGKLFA
Sbjct: 244 KKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFA 303

Query: 301 GHETSAFTAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVID 360
           GHETSA+TAMWAV+FLT HP++FQ+AKEEQE I+ QRPTTQKG+ LSE+KQMK+LYQVID
Sbjct: 304 GHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVID 363

Query: 361 ETLRVSYITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWD 420
           ETLRV  +T MLFREA+ DVEINGK+IP+GWKV+ WFGELY DEK FPSPQEFNPSRWD
Sbjct: 364 ETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420

BLAST of Sed0021386 vs. ExPASy TrEMBL
Match: A0A6J1L1N7 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500298 PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 5.9e-173
Identity = 298/419 (71.12%), Postives = 360/419 (85.92%), Query Frame = 0

Query: 1   MELEKLLVLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYA 60
           ME++  LVL +  FV V G+LKRLNGW+YAAKLG  W KLPPGDM WP LG T+S++K  
Sbjct: 4   MEVKNWLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYIKNF 63

Query: 61  STGRLPNFLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQA 120
            +G+  NF+  F++ R+GK +MYKTHLLG ATIIV +SELCRQVLGDE+ F PSLPK+  
Sbjct: 64  ISGQPSNFIANFYI-RYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNIN 123

Query: 121 MLTGRKSLFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIE 180
           MLTGRKSL  V KAEH+RLRRLT AP+SGH ALEMYI+HIE TV++ LEEW+SMKN PIE
Sbjct: 124 MLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKN-PIE 183

Query: 181 LLTEMKKLTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKAR 240
           LL+E+KKLTFKIIWNIFMGSSS++ + GE+E LF++VA GF+S PINFPGF  H+ALKAR
Sbjct: 184 LLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKAR 243

Query: 241 KKMHEVLQSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFA 300
           KK+ ++LQSI++QKRLV+KR+G +WEAKD MD+LMEV++EDGE LDDETIRDLIFGKLFA
Sbjct: 244 KKLLDILQSIIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFA 303

Query: 301 GHETSAFTAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVID 360
           GHETSA+TAMWAV+FLT HP++FQ+AKEEQE I+ QRPTTQKG+ LSE+K+MK+L Q+ID
Sbjct: 304 GHETSAYTAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQIID 363

Query: 361 ETLRVSYITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWD 420
           ETLRV  +T MLFREA+ DVEINGK+IP+GWKV+ WFGELY DEK FPSP+EFNPSRWD
Sbjct: 364 ETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWD 420

BLAST of Sed0021386 vs. ExPASy TrEMBL
Match: A0A5D3CIW4 (Beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001790 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.8e-170
Identity = 294/412 (71.36%), Postives = 357/412 (86.65%), Query Frame = 0

Query: 1   MELEKLLVLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYA 60
           MEL++ LVL IG   +++GV+KRLNGW+Y AKLG  W KLPPGDM WP LG TLSF+K  
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  STGRLPNFLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQA 120
           ++G+    L+   ++R+GK++MYKTHLLG A+IIV TSE+CRQVL DEE F PSLP+S  
Sbjct: 61  NSGQPRILLHNLSISRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSIT 120

Query: 121 MLTGRKSLFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIE 180
           +L+GRKSL  V KAEHRRLRRLT AP+SGH ALEMYI+HIEQTVI+ LEEW+SMK +PIE
Sbjct: 121 LLSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMK-KPIE 180

Query: 181 LLTEMKKLTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKAR 240
           LLTE+KKLTFKIIWNIFMGSSSID + GE+EALFAQ+A GF+S PINFPGF FH++LKAR
Sbjct: 181 LLTEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKAR 240

Query: 241 KKMHEVLQSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFA 300
           K++  +LQSI+NQKR+ KK+EG+NWEAKD MD+L+E+++EDGE LDDETIRDLIFGKLFA
Sbjct: 241 KELLSLLQSIINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFA 300

Query: 301 GHETSAFTAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVID 360
           GHETSA+TAMWAV+FLTNHP++ Q+ KEEQE+I+++RPTTQKGL+LSE+KQMK+LYQVID
Sbjct: 301 GHETSAYTAMWAVLFLTNHPHILQRTKEEQEDIVKRRPTTQKGLSLSEVKQMKFLYQVID 360

Query: 361 ETLRVSYITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQE 413
           ETLRV  IT M+FREA+ DVEINGK+IP+GWKV+ WFGELY DEK FPSP E
Sbjct: 361 ETLRVGSITFMVFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKQFPSPHE 411

BLAST of Sed0021386 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 362.5 bits (929), Expect = 5.1e-100
Identity = 189/416 (45.43%), Postives = 272/416 (65.38%), Query Frame = 0

Query: 8   VLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYASTGRLPN 67
           ++ +GCF + + +++++N W Y + LG     LPPGD+ WP +G  LSF++   T    +
Sbjct: 14  LMVLGCFGLNW-LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDS 73

Query: 68  FLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQAMLTGRKS 127
           F  T  + R+G   +YK H+ GN +IIV TS+ CR+VL D++ F P  P S   L GRKS
Sbjct: 74  FTRT-LIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKS 133

Query: 128 LFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIELLTEMKK 187
              ++  EH+RLRRLT APV+GH+AL  YI +IE+ VI  L++W+ M     E LT ++K
Sbjct: 134 FVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE--FEFLTHLRK 193

Query: 188 LTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKARKKMHEVL 247
           LTF+II  IF+ S S +     LE  +  +  G  +  +N PGF +HRALKARK +    
Sbjct: 194 LTFRIIMYIFLSSES-ENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAF 253

Query: 248 QSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFAGHETSAF 307
           QSI+ ++R  +K+   +   KD +D L+ V++EDG+ LDDE I D++   L AGHE+S  
Sbjct: 254 QSIVTERRNQRKQNILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGH 313

Query: 308 TAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVIDETLRVSY 367
           T MWA +FL  HP V Q+AK EQE I++ RP  QKGL+L E ++M++L QV+DETLRV  
Sbjct: 314 TIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVIT 373

Query: 368 ITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWDVSFI 424
            +   FREA TDVE+NG LIP+GWKV+TWF +++ D + FP P++F+P+RWD  F+
Sbjct: 374 FSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFV 423

BLAST of Sed0021386 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 351.3 bits (900), Expect = 1.2e-96
Identity = 188/412 (45.63%), Postives = 269/412 (65.29%), Query Frame = 0

Query: 8   VLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYASTGRLPN 67
           ++ +G FV+ + VLKR+N W Y +KLG K   LPPGD+ WP +G   SF++   T    +
Sbjct: 13  LIILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPES 72

Query: 68  FLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQAMLTGRKS 127
           F+ + ++ R+G+  +YK H+ G   ++V T E CR+VL D++ F    PKS   L GRKS
Sbjct: 73  FIQS-YITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 132

Query: 128 LFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIELLTEMKK 187
              ++  EH+RLRRLT+APV+G +AL +YI  IE+TV  DLE+WS M    IE L+ ++K
Sbjct: 133 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE--IEFLSHLRK 192

Query: 188 LTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKARKKMHEVL 247
           LTFK+I  IF+ S S +     LE  +  +  G  +  IN PGF +HRALKARKK+    
Sbjct: 193 LTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 252

Query: 248 QSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFAGHETSAF 307
           QSI+  +R  +K+   +   KD +D L++V++E+G  LDDE I DL+   L AGHE+S  
Sbjct: 253 QSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGH 312

Query: 308 TAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVIDETLRVSY 367
             MWA I +  HP + QKAKEEQE I+++R   QK LTL E ++M YL QVIDETLRV  
Sbjct: 313 LTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVIT 372

Query: 368 ITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWD 420
            +   FREA +DV+++G +IP+GWKV+TWF  ++ D + +P P++F+PSRW+
Sbjct: 373 FSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWE 417

BLAST of Sed0021386 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 351.3 bits (900), Expect = 1.2e-96
Identity = 188/412 (45.63%), Postives = 269/412 (65.29%), Query Frame = 0

Query: 8   VLSIGCFVIVYGVLKRLNGWFYAAKLGNKWAKLPPGDMSWPALGCTLSFVKYASTGRLPN 67
           ++ +G FV+ + VLKR+N W Y +KLG K   LPPGD+ WP +G   SF++   T    +
Sbjct: 13  LIILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPES 72

Query: 68  FLNTFFMARHGKAEMYKTHLLGNATIIVYTSELCRQVLGDEETFMPSLPKSQAMLTGRKS 127
           F+ + ++ R+G+  +YK H+ G   ++V T E CR+VL D++ F    PKS   L GRKS
Sbjct: 73  FIQS-YITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 132

Query: 128 LFTVTKAEHRRLRRLTTAPVSGHKALEMYISHIEQTVINDLEEWSSMKNRPIELLTEMKK 187
              ++  EH+RLRRLT+APV+G +AL +YI  IE+TV  DLE+WS M    IE L+ ++K
Sbjct: 133 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE--IEFLSHLRK 192

Query: 188 LTFKIIWNIFMGSSSIDFTFGELEALFAQVAEGFVSFPINFPGFMFHRALKARKKMHEVL 247
           LTFK+I  IF+ S S +     LE  +  +  G  +  IN PGF +HRALKARKK+    
Sbjct: 193 LTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 252

Query: 248 QSILNQKRLVKKREGKNWEAKDTMDMLMEVREEDGEGLDDETIRDLIFGKLFAGHETSAF 307
           QSI+  +R  +K+   +   KD +D L++V++E+G  LDDE I DL+   L AGHE+S  
Sbjct: 253 QSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGH 312

Query: 308 TAMWAVIFLTNHPNVFQKAKEEQENIIRQRPTTQKGLTLSEIKQMKYLYQVIDETLRVSY 367
             MWA I +  HP + QKAKEEQE I+++R   QK LTL E ++M YL QVIDETLRV  
Sbjct: 313 LTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVIT 372

Query: 368 ITPMLFREALTDVEINGKLIPRGWKVVTWFGELYKDEKHFPSPQEFNPSRWD 420
            +   FREA +DV+++G +IP+GWKV+TWF  ++ D + +P P++F+PSRW+
Sbjct: 373 FSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWE 417

BLAST of Sed0021386 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 201.1 bits (510), Expect = 1.9e-51
Identity = 121/385 (31.43%), Postives = 204/385 (52.99%), Query Frame = 0

Query: 39  KLPPGDMSWPALGCTLSFVKYASTGRLPNFLNTFFMARHGK-AEMYKTHLLGNATIIVYT 98
           +LPPG M  P +G TL    Y          N+FF  R  K  +++KTH+LG   +++ +
Sbjct: 48  RLPPGSMGLPYIGETLRL--YTENP------NSFFATRQNKYGDIFKTHILGCPCVMISS 107

Query: 99  SELCRQVL-GDEETFMPSLPKSQAMLTGRKSLFTVTKAEHRRLRRLTTAPVSGHKALEMY 158
            E  R VL      F P+ P S+  + G ++LF      H  L+RL  +      AL   
Sbjct: 108 PEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFM-PSALRPT 167

Query: 159 ISHIEQTVINDLEEWSSMKNRPIELLTEMKKLTFKIIWNIFMGSSSIDFTFGELEALFAQ 218
           +SHIE  V+  L  W+S K+  I  L  MK+  F +      G      T   ++ L+ +
Sbjct: 168 VSHIELLVLQTLSSWTSQKS--INTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQR 227

Query: 219 VAEGFVSFPINFPGFMFHRALKARKKMHEVLQSILNQKRLVKKREGKNWEAKDTMDMLME 278
           +  G+ S P++ PG +FH+++KAR ++ E L+ ++ ++R   + EG        + +L+ 
Sbjct: 228 LERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG------GLLGVLLG 287

Query: 279 VREEDGEGLDDETIRDLIFGKLFAGHETSAFTAMWAVIFLTNHPNVFQKAKEEQENIIRQ 338
            +++   GL D  I D I G +FA  +T+A    W + +L +HPN+ Q+   EQ +I ++
Sbjct: 288 AKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQK 347

Query: 339 RPTTQKGLTLSEIKQMKYLYQVIDETLRVSYITPMLFREALTDVEINGKLIPRGWKVVTW 398
                + ++  + ++M    +VI ETLR + +    FREA+ DVE +G LIP+GWKV+  
Sbjct: 348 IKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPL 407

Query: 399 FGELYKDEKHFPSPQEFNPSRWDVS 422
           F  ++   + FP P++F+PSR++V+
Sbjct: 408 FRRIHHSSEFFPDPEKFDPSRFEVA 415

BLAST of Sed0021386 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 201.1 bits (510), Expect = 1.9e-51
Identity = 121/385 (31.43%), Postives = 204/385 (52.99%), Query Frame = 0

Query: 39  KLPPGDMSWPALGCTLSFVKYASTGRLPNFLNTFFMARHGK-AEMYKTHLLGNATIIVYT 98
           +LPPG M  P +G TL    Y          N+FF  R  K  +++KTH+LG   +++ +
Sbjct: 48  RLPPGSMGLPYIGETLRL--YTENP------NSFFATRQNKYGDIFKTHILGCPCVMISS 107

Query: 99  SELCRQVL-GDEETFMPSLPKSQAMLTGRKSLFTVTKAEHRRLRRLTTAPVSGHKALEMY 158
            E  R VL      F P+ P S+  + G ++LF      H  L+RL  +      AL   
Sbjct: 108 PEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFM-PSALRPT 167

Query: 159 ISHIEQTVINDLEEWSSMKNRPIELLTEMKKLTFKIIWNIFMGSSSIDFTFGELEALFAQ 218
           +SHIE  V+  L  W+S K+  I  L  MK+  F +      G      T   ++ L+ +
Sbjct: 168 VSHIELLVLQTLSSWTSQKS--INTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQR 227

Query: 219 VAEGFVSFPINFPGFMFHRALKARKKMHEVLQSILNQKRLVKKREGKNWEAKDTMDMLME 278
           +  G+ S P++ PG +FH+++KAR ++ E L+ ++ ++R   + EG        + +L+ 
Sbjct: 228 LERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG------GLLGVLLG 287

Query: 279 VREEDGEGLDDETIRDLIFGKLFAGHETSAFTAMWAVIFLTNHPNVFQKAKEEQENIIRQ 338
            +++   GL D  I D I G +FA  +T+A    W + +L +HPN+ Q+   EQ +I ++
Sbjct: 288 AKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQK 347

Query: 339 RPTTQKGLTLSEIKQMKYLYQVIDETLRVSYITPMLFREALTDVEINGKLIPRGWKVVTW 398
                + ++  + ++M    +VI ETLR + +    FREA+ DVE +G LIP+GWKV+  
Sbjct: 348 IKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPL 407

Query: 399 FGELYKDEKHFPSPQEFNPSRWDVS 422
           F  ++   + FP P++F+PSR++V+
Sbjct: 408 FRRIHHSSEFFPDPEKFDPSRFEVA 415