Sed0021222 (gene) Chayote v1

Overview


Name	Sed0021222
Type	gene
Organism	Sechium edule (Chayote v1)
Description	aspartic proteinase PCS1
Location	LG04: 26243415 .. 26245332 (+)
RNA-Seq Expression	Sed0021222
Synteny	Sed0021222

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTGCTTCTGCACCGAATCGTGAACACCAACCGGAGATCTTACTGTGAATTTCTTTTTCTTTTTTTCTTTTCCGAATTTATTCTCTTTTTTTTTTCTTAAATAATTGGGATTTATACTCTTGGATCTTCGCAAGGGAGAAGTTTCTCAGGTTTTCAGAACACTCTGTTCTCATAAACCAAAACAGAGCCAACAAAAACACCTTCACATTTCTTCACTCTTCTTCTTCCAACTGCTCCAACTGTTTCCCAACTCCAAAAAATGATCAATTATTATACAAACAACCAATTCCCCAAATCTTGTTTCCTATTCTTTTTCTTGTTCTTTCAAAATCTCAGTCTTTCTTCATCAATTAACCCAGCTATGGTTTTTCCACTCAAAAGCCAAGTAATTCCGCAGGAATCCGTCCGGCGATCCCCAGACAAGCTCCCTTTCCGGCACAACATCAGTCTCACCGTCGCTCTGACCGTCGGAACGCCGCCGCAGAACGTCACTATGGTAATCGACACCGGCAGCGAACTCTCATGGCTCCACTGCAACAGATCACAAAATTCTTCCTCTTCATCTTCGACGTTCAATCCGGTCTTGTCATCCTCGTACGCGCCGATCCCGTGTTCCTCCGCCACCTGTACCGACCAAACTCGGGATTTTCCGATTCCGGCATCCTGCGATTCGAATCAGCTCTGTCACGCCACTCTGTCTTACGCCGACGCCTCCTCTTCCGAAGGGATTCTCGCCTCCGATACGTTCTACCTCGGAAATTCCGGAATTTCGGATGTGGTTTTTGGTTGTATGGATTCAATTTTCAGTTCCAACAATGAAGAAGACTCCAAAAACACCGGATTAATGGGCATGAATCGTGGATCTTTATCTTTCGTTTCCCAAATGGGTTTCCCCAAATTTTCTTATTGCATATCGGAATATGATTTTTCCGGTTTGTTACTTCTCGGAGATGCTAATTTCTCTTGGTTAGCACCGTTGAATTACACTCCACTAATCGAAATCTCAACGCCATTGCCGTATTTCGATCGGGTCGCTTACACGGTTCAATTGGAAGGGATCAAAGTTTCCAACAAATTACTTCCGATACCGGAATCTGTCTTCGAACCGGACCACACCGGAGCCGGTCAGACCATGGTCGACTCCGGAACCCAGTTCACTTTCCTTCTTGGACCGGCTTACACCGCGCTCCGAGACGAGTTTCTGAACCAAACCGCCGGTTCGCTCCGGGTTTTGGAGGATCCGAATTTCGTTTTCCAAGGAGCTATGGATCTTTGCTACCGGGTTCCGACGAACCAAACCCGTCTTCCGCCGCTGCCGGCGGTGACGCTGGTGTTTCGCGGGGCCGAGATGACGGTTACCGGTGACCGGATTCTGTACCGGGTGGCCGGAGAAATTAGAGGGAACGATACGATCCATTGCTTCACATTCGGAAATTCGGATCTTTTGGGCGTTGAAGCGTACGTGATTGGGCATCTTCATCAGCAAAACGTGTGGATGGAATTCGATCTCAAAAAATCGCGGATCGGGTTGGCGGAGATCCGGTGCGATTTAGCGGGTCAGAAGCTGGGTATAGGCCTGTAATGTAAATGACCCGGACCCATTTGATTGAAGCGTTTTAACTATCGGGTCAGAACTTACGGGTTATGGTCCATGCTATTATAGTGTTCCTTCCGCTTTTAATATATATGTGGGTCCCATACCATCGGTAATATTGAGAAGAAAAAAAGAAAAAGAAATGTGATTTTGATCTGACGGGTGTAATGAGTCTGAGTGATCTGCGATGTAGACACGTGTAAGAGAGATTTTCTTTTTCTGCCCTACCTGTACATTTTTAATATTCATGAGCTTTAATGTAATAAAGCTATGTGTATGAAGAGTAAAATATTATTTATGATATGTTTTGAGGGGATATTTTGA

mRNA sequence

TGTGCTTCTGCACCGAATCGTGAACACCAACCGGAGATCTTACTGTGAATTTCTTTTTCTTTTTTTCTTTTCCGAATTTATTCTCTTTTTTTTTTCTTAAATAATTGGGATTTATACTCTTGGATCTTCGCAAGGGAGAAGTTTCTCAGGTTTTCAGAACACTCTGTTCTCATAAACCAAAACAGAGCCAACAAAAACACCTTCACATTTCTTCACTCTTCTTCTTCCAACTGCTCCAACTGTTTCCCAACTCCAAAAAATGATCAATTATTATACAAACAACCAATTCCCCAAATCTTGTTTCCTATTCTTTTTCTTGTTCTTTCAAAATCTCAGTCTTTCTTCATCAATTAACCCAGCTATGGTTTTTCCACTCAAAAGCCAAGTAATTCCGCAGGAATCCGTCCGGCGATCCCCAGACAAGCTCCCTTTCCGGCACAACATCAGTCTCACCGTCGCTCTGACCGTCGGAACGCCGCCGCAGAACGTCACTATGGTAATCGACACCGGCAGCGAACTCTCATGGCTCCACTGCAACAGATCACAAAATTCTTCCTCTTCATCTTCGACGTTCAATCCGGTCTTGTCATCCTCGTACGCGCCGATCCCGTGTTCCTCCGCCACCTGTACCGACCAAACTCGGGATTTTCCGATTCCGGCATCCTGCGATTCGAATCAGCTCTGTCACGCCACTCTGTCTTACGCCGACGCCTCCTCTTCCGAAGGGATTCTCGCCTCCGATACGTTCTACCTCGGAAATTCCGGAATTTCGGATGTGGTTTTTGGTTGTATGGATTCAATTTTCAGTTCCAACAATGAAGAAGACTCCAAAAACACCGGATTAATGGGCATGAATCGTGGATCTTTATCTTTCGTTTCCCAAATGGGTTTCCCCAAATTTTCTTATTGCATATCGGAATATGATTTTTCCGGTTTGTTACTTCTCGGAGATGCTAATTTCTCTTGGTTAGCACCGTTGAATTACACTCCACTAATCGAAATCTCAACGCCATTGCCGTATTTCGATCGGGTCGCTTACACGGTTCAATTGGAAGGGATCAAAGTTTCCAACAAATTACTTCCGATACCGGAATCTGTCTTCGAACCGGACCACACCGGAGCCGGTCAGACCATGGTCGACTCCGGAACCCAGTTCACTTTCCTTCTTGGACCGGCTTACACCGCGCTCCGAGACGAGTTTCTGAACCAAACCGCCGGTTCGCTCCGGGTTTTGGAGGATCCGAATTTCGTTTTCCAAGGAGCTATGGATCTTTGCTACCGGGTTCCGACGAACCAAACCCGTCTTCCGCCGCTGCCGGCGGTGACGCTGGTGTTTCGCGGGGCCGAGATGACGGTTACCGGTGACCGGATTCTGTACCGGGTGGCCGGAGAAATTAGAGGGAACGATACGATCCATTGCTTCACATTCGGAAATTCGGATCTTTTGGGCGTTGAAGCGTACGTGATTGGGCATCTTCATCAGCAAAACGTGTGGATGGAATTCGATCTCAAAAAATCGCGGATCGGGTTGGCGGAGATCCGGTGCGATTTAGCGGGTCAGAAGCTGGGTATAGGCCTGTAATGTAAATGACCCGGACCCATTTGATTGAAGCGTTTTAACTATCGGGTCAGAACTTACGGGTTATGGTCCATGCTATTATAGTGTTCCTTCCGCTTTTAATATATATGTGGGTCCCATACCATCGGTAATATTGAGAAGAAAAAAAGAAAAAGAAATGTGATTTTGATCTGACGGGTGTAATGAGTCTGAGTGATCTGCGATGTAGACACGTGTAAGAGAGATTTTCTTTTTCTGCCCTACCTGTACATTTTTAATATTCATGAGCTTTAATGTAATAAAGCTATGTGTATGAAGAGTAAAATATTATTTATGATATGTTTTGAGGGGATATTTTGA

Coding sequence (CDS)

ATGATCAATTATTATACAAACAACCAATTCCCCAAATCTTGTTTCCTATTCTTTTTCTTGTTCTTTCAAAATCTCAGTCTTTCTTCATCAATTAACCCAGCTATGGTTTTTCCACTCAAAAGCCAAGTAATTCCGCAGGAATCCGTCCGGCGATCCCCAGACAAGCTCCCTTTCCGGCACAACATCAGTCTCACCGTCGCTCTGACCGTCGGAACGCCGCCGCAGAACGTCACTATGGTAATCGACACCGGCAGCGAACTCTCATGGCTCCACTGCAACAGATCACAAAATTCTTCCTCTTCATCTTCGACGTTCAATCCGGTCTTGTCATCCTCGTACGCGCCGATCCCGTGTTCCTCCGCCACCTGTACCGACCAAACTCGGGATTTTCCGATTCCGGCATCCTGCGATTCGAATCAGCTCTGTCACGCCACTCTGTCTTACGCCGACGCCTCCTCTTCCGAAGGGATTCTCGCCTCCGATACGTTCTACCTCGGAAATTCCGGAATTTCGGATGTGGTTTTTGGTTGTATGGATTCAATTTTCAGTTCCAACAATGAAGAAGACTCCAAAAACACCGGATTAATGGGCATGAATCGTGGATCTTTATCTTTCGTTTCCCAAATGGGTTTCCCCAAATTTTCTTATTGCATATCGGAATATGATTTTTCCGGTTTGTTACTTCTCGGAGATGCTAATTTCTCTTGGTTAGCACCGTTGAATTACACTCCACTAATCGAAATCTCAACGCCATTGCCGTATTTCGATCGGGTCGCTTACACGGTTCAATTGGAAGGGATCAAAGTTTCCAACAAATTACTTCCGATACCGGAATCTGTCTTCGAACCGGACCACACCGGAGCCGGTCAGACCATGGTCGACTCCGGAACCCAGTTCACTTTCCTTCTTGGACCGGCTTACACCGCGCTCCGAGACGAGTTTCTGAACCAAACCGCCGGTTCGCTCCGGGTTTTGGAGGATCCGAATTTCGTTTTCCAAGGAGCTATGGATCTTTGCTACCGGGTTCCGACGAACCAAACCCGTCTTCCGCCGCTGCCGGCGGTGACGCTGGTGTTTCGCGGGGCCGAGATGACGGTTACCGGTGACCGGATTCTGTACCGGGTGGCCGGAGAAATTAGAGGGAACGATACGATCCATTGCTTCACATTCGGAAATTCGGATCTTTTGGGCGTTGAAGCGTACGTGATTGGGCATCTTCATCAGCAAAACGTGTGGATGGAATTCGATCTCAAAAAATCGCGGATCGGGTTGGCGGAGATCCGGTGCGATTTAGCGGGTCAGAAGCTGGGTATAGGCCTGTAA

Protein sequence

MINYYTNNQFPKSCFLFFFLFFQNLSLSSSINPAMVFPLKSQVIPQESVRRSPDKLPFRHNISLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVLSSSYAPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLNYTPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGIGL

Homology

[Close]

BLAST of Sed0021222 vs. NCBI nr
Match: XP_022948372.1 (aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022948381.1 aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 798.9 bits (2062), Expect = 2.2e-227
Identity = 396/438 (90.41%), Postives = 412/438 (94.06%), Query Frame = 0


Query: 7   NNQFPKSCFLFFFL----FFQNLSLSSSINPAMVFPLKSQVIPQESVRRSPDKLPFRHNI 66
           NN F K CF FF +     FQN  L  S+NPA++ PLK+QVIP ESV RSPDKLPFRHN+
Sbjct: 12  NNPFLKYCFSFFLISLVFVFQNPLLCFSLNPALILPLKTQVIPPESVPRSPDKLPFRHNV 71

Query: 67  SLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVLSSSYAPIPCSSAT 126
           SLTV+LTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPV SSSY PIPCSS+T
Sbjct: 72  SLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVQSSSYTPIPCSSST 131

Query: 127 CTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMDSIF 186
           CTDQTRDFPIPASCDSNQLCHATLSYADASSSEG LA+DTFY+GNSGI +VVFGCMDSIF
Sbjct: 132 CTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGIPNVVFGCMDSIF 191

Query: 187 SSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLNY 246
           SSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDF GLLLLGDANFSWLAPLNY
Sbjct: 192 SSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFPGLLLLGDANFSWLAPLNY 251

Query: 247 TPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFL 306
           TP+I+ISTPLPYFDRVAYTVQL+GIKVSNKLLPIPES FEPDHTGAGQTMVDSGTQFTFL
Sbjct: 252 TPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESAFEPDHTGAGQTMVDSGTQFTFL 311

Query: 307 LGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFRGA 366
           LG AYT LRDEFLNQTAGSLRV EDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFRGA
Sbjct: 312 LGQAYTVLRDEFLNQTAGSLRVSEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFRGA 371

Query: 367 EMTVTGDRILYRVAGEIRGNDTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRI 426
           EMTVTGDRILYRVAGEIRGNDTI+CFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRI
Sbjct: 372 EMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRI 431

Query: 427 GLAEIRCDLAGQKLGIGL 441
           GLAEIRCDLAGQKLG+GL
Sbjct: 432 GLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Sed0021222 vs. NCBI nr
Match: XP_038905417.1 (aspartic proteinase PCS1 [Benincasa hispida])

HSP 1 Score: 798.9 bits (2062), Expect = 2.2e-227
Identity = 397/439 (90.43%), Postives = 417/439 (94.99%), Query Frame = 0


Query: 6   TNNQFPKSCFLFFF--LF--FQNLSLSSSINPAMVFPLKSQVIPQESVRRSPDKLPFRHN 65
           ++N+F K CF+FFF  LF  FQN  L SS+NPA+V PLK+QVIP ESVRRSPDKLPFRHN
Sbjct: 11  SSNKFLKYCFIFFFCTLFCVFQNSKLCSSLNPALVLPLKTQVIPPESVRRSPDKLPFRHN 70

Query: 66  ISLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVLSSSYAPIPCSSA 125
           ISLTV+LTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPV SSSY PIPCSS+
Sbjct: 71  ISLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVRSSSYTPIPCSSS 130

Query: 126 TCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMDSI 185
           TCTDQTRDFPI  SCDSNQ CHATLSYADASSSEG LA+DTFY+GNSGI +VVFGCMDSI
Sbjct: 131 TCTDQTRDFPIRPSCDSNQFCHATLSYADASSSEGNLATDTFYIGNSGIPNVVFGCMDSI 190

Query: 186 FSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLN 245
           FSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLN
Sbjct: 191 FSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLN 250

Query: 246 YTPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTF 305
           YTPLIE+STPLPYFDRVAYTVQLEGIKVS+KLLPIPESVFEPDHTGAGQTMVDSGTQFTF
Sbjct: 251 YTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQTMVDSGTQFTF 310

Query: 306 LLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFRG 365
           LLGPAY ALRDEF+NQTAGS+RVLEDPNFVFQGAMDLCYRVP NQTRLPPLP+VTLVFRG
Sbjct: 311 LLGPAYAALRDEFVNQTAGSIRVLEDPNFVFQGAMDLCYRVPINQTRLPPLPSVTLVFRG 370

Query: 366 AEMTVTGDRILYRVAGEIRGNDTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSR 425
           AEMTVTGDRILYRV GE RGND+IHCFTFGNSDLLGVEA+VIGHLHQQNVWMEFDLKKSR
Sbjct: 371 AEMTVTGDRILYRVPGERRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKSR 430

Query: 426 IGLAEIRCDLAGQKLGIGL 441
           IGLAEIRCDLAGQKLG+GL
Sbjct: 431 IGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Sed0021222 vs. NCBI nr
Match: XP_022983486.1 (aspartic proteinase PCS1 [Cucurbita maxima])

HSP 1 Score: 798.9 bits (2062), Expect = 2.2e-227
Identity = 395/439 (89.98%), Postives = 417/439 (94.99%), Query Frame = 0


Query: 6   TNNQFPKSCFLFF----FLFFQNLSLSSSINPAMVFPLKSQVIPQESVRRSPDKLPFRHN 65
           +N++F KS F FF    F  FQNL L SS+NPA++ PLK+QVIP ES+RRSPDKLPFRHN
Sbjct: 11  SNHKFLKSFFPFFLCILFSVFQNLILCSSLNPALLLPLKTQVIPPESIRRSPDKLPFRHN 70

Query: 66  ISLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVLSSSYAPIPCSSA 125
           +SLTV+LTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNP  SSSY PIPCSS+
Sbjct: 71  VSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPAGSSSYTPIPCSSS 130

Query: 126 TCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMDSI 185
           TCTDQTRDFPIPASCDSN LCHATLSYADASSSEG LA+DTFY+GNSGIS+VVFGCMDSI
Sbjct: 131 TCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGISNVVFGCMDSI 190

Query: 186 FSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLN 245
           FSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLN
Sbjct: 191 FSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLN 250

Query: 246 YTPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTF 305
           YTPLIE+STPLPYFDRVAYTVQLEGIKVS+KLLPIPESVFEPDHTGAGQTMVDSGTQFTF
Sbjct: 251 YTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQTMVDSGTQFTF 310

Query: 306 LLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFRG 365
           LLGPAYTALRDEFLN+TAGS+RV ED NFVFQGAMDLCYRVP NQTRLPPLP+VTLVFRG
Sbjct: 311 LLGPAYTALRDEFLNRTAGSIRVFEDSNFVFQGAMDLCYRVPMNQTRLPPLPSVTLVFRG 370

Query: 366 AEMTVTGDRILYRVAGEIRGNDTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSR 425
           AEMTVTGDRILYRV GEIRGND+IHCFTFGNSDLLGVEA+VIGHLHQQNVWMEFDLKKSR
Sbjct: 371 AEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKSR 430

Query: 426 IGLAEIRCDLAGQKLGIGL 441
           IGLAEIRCDLAGQKLG+GL
Sbjct: 431 IGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Sed0021222 vs. NCBI nr
Match: XP_022145548.1 (aspartic proteinase PCS1 [Momordica charantia])

HSP 1 Score: 794.7 bits (2051), Expect = 4.2e-226
Identity = 394/443 (88.94%), Postives = 420/443 (94.81%), Query Frame = 0


Query: 5   YTNNQFPKSCFLFFFLF----FQNLSLSSSINPAMVFPLKSQVIPQESVRRSPDKLPFRH 64
           ++N+ F KSCF FF       FQN  L SS+NPA+V PLK+QVIP ESVRRSPDKLPFRH
Sbjct: 9   FSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRRSPDKLPFRH 68

Query: 65  NISLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSS-SSSTFNPVLSSSYAPIPCS 124
           N+SLTV+LTVGTPPQNVTMVIDTGSELSWLHCN+SQNSSS SSSTFNP+ SSSY+PIPCS
Sbjct: 69  NVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRSSSYSPIPCS 128

Query: 125 SATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMD 184
           S+TCTDQTRDFPIPASCDSNQLCHATLSYADASSSEG LA+DTFY+GNSGIS+VVFGCMD
Sbjct: 129 SSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGISNVVFGCMD 188

Query: 185 SIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSW--L 244
           SIFSSNNEED+KNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDAN+SW  L
Sbjct: 189 SIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANYSWLGL 248

Query: 245 APLNYTPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGT 304
           APLNYTPLI++STPLPYFDRVAYTV LEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGT
Sbjct: 249 APLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGT 308

Query: 305 QFTFLLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTL 364
           QFTFLLGPAYTALRDEFLNQTAGS+RVL+DPNFVFQGAMDLCYRVP NQ+RLPPLPAVTL
Sbjct: 309 QFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSRLPPLPAVTL 368

Query: 365 VFRGAEMTVTGDRILYRVAGEIRGNDTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDL 424
           VFRGAEMTV+GDRILYRV GEIRGND+IHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDL
Sbjct: 369 VFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDL 428

Query: 425 KKSRIGLAEIRCDLAGQKLGIGL 441
           +KSRIGLAEIRCDLAGQKLG+GL
Sbjct: 429 QKSRIGLAEIRCDLAGQKLGMGL 451

[Close]

BLAST of Sed0021222 vs. NCBI nr
Match: XP_008442528.1 (PREDICTED: aspartic proteinase PCS1 [Cucumis melo] >KAA0044101.1 aspartic proteinase PCS1 [Cucumis melo var. makuwa] >TYK25036.1 aspartic proteinase PCS1 [Cucumis melo var. makuwa])

HSP 1 Score: 792.7 bits (2046), Expect = 1.6e-225
Identity = 397/440 (90.23%), Postives = 416/440 (94.55%), Query Frame = 0


Query: 7   NNQFPKSCFLFFF--LF--FQNLSLSSSINPAMVFPLKSQVIPQESVRRSPDKLPFRHNI 66
           N +F KSCFLFFF  LF  F N+ L SS+NPA+V PLK+QVIP ESVRRSPDKLPFRHNI
Sbjct: 12  NIKFLKSCFLFFFCILFSVFYNIDLCSSVNPALVLPLKTQVIPPESVRRSPDKLPFRHNI 71

Query: 67  SLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQN--SSSSSSTFNPVLSSSYAPIPCSS 126
           SLTV+LTVGTPPQNVTMVIDTGSELSWLHCN SQN  SSSSSSTFNPV SSSY+PIPCSS
Sbjct: 72  SLTVSLTVGTPPQNVTMVIDTGSELSWLHCNTSQNSSSSSSSSTFNPVRSSSYSPIPCSS 131

Query: 127 ATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMDS 186
           +TCTDQTRDFPI  SCDSNQ CHATLSYADASSSEG LA+DTFY+GNSGI +VVFGCMDS
Sbjct: 132 STCTDQTRDFPIRPSCDSNQFCHATLSYADASSSEGNLATDTFYIGNSGIPNVVFGCMDS 191

Query: 187 IFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPL 246
           IFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPL
Sbjct: 192 IFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPL 251

Query: 247 NYTPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFT 306
           NYTPLIE+STPLPYFDRVAYTVQLEGIKV++KLLPIPESVFEPDHTGAGQTMVDSGTQFT
Sbjct: 252 NYTPLIEMSTPLPYFDRVAYTVQLEGIKVAHKLLPIPESVFEPDHTGAGQTMVDSGTQFT 311

Query: 307 FLLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFR 366
           FLLGPAYTALRD FLNQTAGSLR+ EDPNFVFQGAMDLCYRVPTNQTRLPPLP+VTLVFR
Sbjct: 312 FLLGPAYTALRDHFLNQTAGSLRLYEDPNFVFQGAMDLCYRVPTNQTRLPPLPSVTLVFR 371

Query: 367 GAEMTVTGDRILYRVAGEIRGNDTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKS 426
           GAEMTVTGDRILYRV GE RGND+IHCFTFGNSDLLGVEA+VIGHLHQQNVWMEFDLKKS
Sbjct: 372 GAEMTVTGDRILYRVPGERRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKS 431

Query: 427 RIGLAEIRCDLAGQKLGIGL 441
           RIGLAEIRCDLAGQKLG+GL
Sbjct: 432 RIGLAEIRCDLAGQKLGMGL 451

[Close]

BLAST of Sed0021222 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 2.2e-161
Identity = 285/417 (68.35%), Postives = 339/417 (81.29%), Query Frame = 0


Query: 28  SSSINPAMVFPLKSQVIPQESVRRSPDKLPFRHNISLTVALTVGTPPQNVTMVIDTGSEL 87
           SSS +  +V PLK+++ P +   R  DKL F HN++LTV LTVGTPPQN++MVIDTGSEL
Sbjct: 39  SSSSSQTLVLPLKTRITPTD--HRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSEL 98

Query: 88  SWLHCNRSQNSSSSSSTFNPVLSSSYAPIPCSSATCTDQTRDFPIPASCDSNQLCHATLS 147
           SWL CNRS N +  ++ F+P  SSSY+PIPCSS TC  +TRDF IPASCDS++LCHATLS
Sbjct: 99  SWLRCNRSSNPNPVNN-FDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLS 158

Query: 148 YADASSSEGILASDTFYLGNS-GISDVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFV 207
           YADASSSEG LA++ F+ GNS   S+++FGCM S+  S+ EED+K TGL+GMNRGSLSF+
Sbjct: 159 YADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFI 218

Query: 208 SQMGFPKFSYCIS-EYDFSGLLLLGDANFSWLAPLNYTPLIEISTPLPYFDRVAYTVQLE 267
           SQMGFPKFSYCIS   DF G LLLGD+NF+WL PLNYTPLI ISTPLPYFDRVAYTVQL 
Sbjct: 219 SQMGFPKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLT 278

Query: 268 GIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSLRVL 327
           GIKV+ KLLPIP+SV  PDHTGAGQTMVDSGTQFTFLLGP YTALR  FLN+T G L V 
Sbjct: 279 GIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVY 338

Query: 328 EDPNFVFQGAMDLCYRVPTNQTR---LPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGN 387
           EDP+FVFQG MDLCYR+   + R   L  LP V+LVF GAE+ V+G  +LYRV     GN
Sbjct: 339 EDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTVGN 398

Query: 388 DTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGIG 440
           D+++CFTFGNSDL+G+EAYVIGH HQQN+W+EFDL++SRIGLA + CD++GQ+LGIG
Sbjct: 399 DSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

[Close]

BLAST of Sed0021222 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 2.0e-37
Identity = 118/368 (32.07%), Postives = 186/368 (50.54%), Query Frame = 0


Query: 68  LTVGTPPQNVTMVIDTGSELSWLHCNR-SQNSSSSSSTFNPVLSSSYAPIPCSSATCTDQ 127
           L++GTP Q  + ++DTGS+L W  C   +Q  + S+  FNP  SSS++ +PCSS  C  Q
Sbjct: 99  LSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLC--Q 158

Query: 128 TRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMDSIFSSNN 187
               P   +C SN  C  T  Y D S ++G + ++T   G+  I ++ FGC       NN
Sbjct: 159 ALSSP---TC-SNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGC-----GENN 218

Query: 188 E--EDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFS--GLLLLGDANFSWLAPLNY 247
           +        GL+GM RG LS  SQ+   KFSYC++    S    LLLG    S  A    
Sbjct: 219 QGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPN 278

Query: 248 TPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFE-PDHTGAGQTMVDSGTQFTF 307
           T LI+ S+ +P F    Y + L G+ V +  LPI  S F    + G G  ++DSGT  T+
Sbjct: 279 TTLIQ-SSQIPTF----YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTY 338

Query: 308 LLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFRG 367
            +  AY ++R EF++Q   +L V+   +  F    DLC++ P++ + L  +P   + F G
Sbjct: 339 FVNNAYQSVRQEFISQI--NLPVVNGSSSGF----DLCFQTPSDPSNL-QIPTFVMHFDG 398

Query: 368 AEMTVTGDRILYRVAGEIRGNDTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSR 427
            ++ +  +         I  ++ + C   G+S   G+   + G++ QQN+ + +D   S 
Sbjct: 399 GDLELPSENYF------ISPSNGLICLAMGSSS-QGMS--IFGNIQQQNMLVVYDTGNSV 434

Query: 428 IGLAEIRC 430
           +  A  +C
Sbjct: 459 VSFASAQC 434

[Close]

BLAST of Sed0021222 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 4.9e-36
Identity = 106/365 (29.04%), Postives = 175/365 (47.95%), Query Frame = 0


Query: 68  LTVGTPPQNVTMVIDTGSELSWLHCNR-SQNSSSSSSTFNPVLSSSYAPIPCSSATCTDQ 127
           + +GTP  + + ++DTGS+L W  C   +Q  S  +  FNP  SSS++ +PC S  C D 
Sbjct: 100 VAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQD- 159

Query: 128 TRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMDSIFSSNN 187
                +P+   +N  C  T  Y D S+++G +A++TF    S + ++ FGC +    +  
Sbjct: 160 -----LPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGCGE---DNQG 219

Query: 188 EEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFS--GLLLLGDANFSWLAPLNYTP 247
                  GL+GM  G LS  SQ+G  +FSYC++ Y  S    L LG A          T 
Sbjct: 220 FGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTT 279

Query: 248 LIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLG 307
           LI  S    Y     Y + L+GI V    L IP S F+    G G  ++DSGT  T+L  
Sbjct: 280 LIHSSLNPTY-----YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQ 339

Query: 308 PAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFRGAEM 367
            AY A+   F +Q   +L  +++ +      +  C++ P++ + +  +P +++ F G  +
Sbjct: 340 DAYNAVAQAFTDQI--NLPTVDESS----SGLSTCFQQPSDGSTV-QVPEISMQFDGGVL 399

Query: 368 TVTGDRILYRVAGEIRGNDTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGL 427
            +    IL   A      + + C   G+S  LG+   + G++ QQ   + +DL+   +  
Sbjct: 400 NLGEQNILISPA------EGVICLAMGSSSQLGIS--IFGNIQQQETQVLYDLQNLAVSF 435

Query: 428 AEIRC 430
              +C
Sbjct: 460 VPTQC 435

[Close]

BLAST of Sed0021222 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.3e-33
Identity = 119/378 (31.48%), Postives = 172/378 (45.50%), Query Frame = 0


Query: 60  HNISLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVLSSSYAPIPCS 119
           H  +  V   +GTPPQ + MV+DT ++  WL C+     S++S++FN   SS+Y+ + CS
Sbjct: 100 HIGNYVVRAKLGTPPQLMFMVLDTSNDAVWLPCSGCSGCSNASTSFNTNSSSTYSTVSCS 159

Query: 120 SATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMD 179
           +A CT Q R    P+S     +C    SY   SS    L  DT  L    I +  FGC++
Sbjct: 160 TAQCT-QARGLTCPSSSPQPSVCSFNQSYGGDSSFSASLVQDTLTLAPDVIPNFSFGCIN 219

Query: 180 SIFSSNNEEDSKNTGLMGMNRGSLSFVSQ---MGFPKFSYCISEYD---FSGLLLLGDAN 239
           S  +S N    +  GLMG+ RG +S VSQ   +    FSYC+  +    FSG L LG   
Sbjct: 220 S--ASGNSLPPQ--GLMGLGRGPMSLVSQTTSLYSGVFSYCLPSFRSFYFSGSLKLG--L 279

Query: 240 FSWLAPLNYTPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDHTGAGQTMV 299
                 + YTPL+  +   P      Y V L G+ V +  +P+       D      T++
Sbjct: 280 LGQPKSIRYTPLLR-NPRRPSL----YYVNLTGVSVGSVQVPVDPVYLTFDANSGAGTII 339

Query: 300 DSGTQFTFLLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLP 359
           DSGT  T    P Y A+RDEF  Q   S       +F   GA D C+            P
Sbjct: 340 DSGTVITRFAQPVYEAIRDEFRKQVNVS-------SFSTLGAFDTCFSADNENV----AP 399

Query: 360 AVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIHCFTF-GNSDLLGVEAYVIGHLHQQNVW 419
            +TL     ++ +  +  L   +       T+ C +  G          VI +L QQN+ 
Sbjct: 400 KITLHMTSLDLKLPMENTLIHSSA-----GTLTCLSMAGIRQNANAVLNVIANLQQQNLR 449

Query: 420 MEFDLKKSRIGLAEIRCD 431
           + FD+  SRIG+A   C+
Sbjct: 460 ILFDVPNSRIGIAPEPCN 449

[Close]

BLAST of Sed0021222 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 4.3e-32
Identity = 123/385 (31.95%), Postives = 182/385 (47.27%), Query Frame = 0


Query: 63  SLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVLSSSYAPIPCSSAT 122
           S  V   +G+P Q + + +DT ++ +W HC+    +  SSS F P  SSSYA +PCSS+ 
Sbjct: 78  SYVVRAGLGSPSQQLLLALDTSADATWAHCSPC-GTCPSSSLFAPANSSSYASLPCSSSW 137

Query: 123 C-TDQTRDFPIPASCDSNQLCHATL-------SYADASSSEGILASDTFYLGNSGISDVV 182
           C   Q +  P P          ATL        +ADA S +  LASDT  LG   I +  
Sbjct: 138 CPLFQGQACPAPQGGGDAAPPPATLPTCAFSKPFADA-SFQAALASDTLRLGKDAIPNYT 197

Query: 183 FGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGF---PKFSYCISEYD---FSGLLL 242
           FGC+ S+  +    +    GL+G+ RG ++ +SQ G      FSYC+  Y    FSG L 
Sbjct: 198 FGCVSSV--TGPTTNMPRQGLLGLGRGPMALLSQAGSLYNGVFSYCLPSYRSYYFSGSLR 257

Query: 243 LGDANFSWLAPLNYTPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPD-HTG 302
           LG A       + YTP++      P+   + Y V + G+ V +  + +P   F  D  TG
Sbjct: 258 LG-AGGGQPRSVRYTPMLR----NPHRSSL-YYVNVTGLSVGHAWVKVPAGSFAFDAATG 317

Query: 303 AGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQT 362
           AG T+VDSGT  T    P Y ALR+EF  Q A          +   GA D C+   T++ 
Sbjct: 318 AG-TVVDSGTVITRWTAPVYAALREEFRRQVAA------PSGYTSLGAFDTCFN--TDEV 377

Query: 363 RLPPLPAVTL-VFRGAEMTVTGDRILYRVAGEIRGNDTIHCFTFGNS-DLLGVEAYVIGH 422
                PAVT+ +  G ++ +  +  L   +        + C     +   +     VI +
Sbjct: 378 AAGGAPAVTVHMDGGVDLALPMENTLIHSSA-----TPLACLAMAEAPQNVNSVVNVIAN 437

Query: 423 LHQQNVWMEFDLKKSRIGLAEIRCD 431
           L QQN+ + FD+  SR+G A+  C+
Sbjct: 438 LQQQNIRVVFDVANSRVGFAKESCN 438

[Close]

BLAST of Sed0021222 vs. ExPASy TrEMBL
Match: A0A6J1G928 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111452070 PE=3 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 1.1e-227
Identity = 396/438 (90.41%), Postives = 412/438 (94.06%), Query Frame = 0


Query: 7   NNQFPKSCFLFFFL----FFQNLSLSSSINPAMVFPLKSQVIPQESVRRSPDKLPFRHNI 66
           NN F K CF FF +     FQN  L  S+NPA++ PLK+QVIP ESV RSPDKLPFRHN+
Sbjct: 12  NNPFLKYCFSFFLISLVFVFQNPLLCFSLNPALILPLKTQVIPPESVPRSPDKLPFRHNV 71

Query: 67  SLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVLSSSYAPIPCSSAT 126
           SLTV+LTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPV SSSY PIPCSS+T
Sbjct: 72  SLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVQSSSYTPIPCSSST 131

Query: 127 CTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMDSIF 186
           CTDQTRDFPIPASCDSNQLCHATLSYADASSSEG LA+DTFY+GNSGI +VVFGCMDSIF
Sbjct: 132 CTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGIPNVVFGCMDSIF 191

Query: 187 SSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLNY 246
           SSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDF GLLLLGDANFSWLAPLNY
Sbjct: 192 SSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFPGLLLLGDANFSWLAPLNY 251

Query: 247 TPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFL 306
           TP+I+ISTPLPYFDRVAYTVQL+GIKVSNKLLPIPES FEPDHTGAGQTMVDSGTQFTFL
Sbjct: 252 TPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESAFEPDHTGAGQTMVDSGTQFTFL 311

Query: 307 LGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFRGA 366
           LG AYT LRDEFLNQTAGSLRV EDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFRGA
Sbjct: 312 LGQAYTVLRDEFLNQTAGSLRVSEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFRGA 371

Query: 367 EMTVTGDRILYRVAGEIRGNDTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRI 426
           EMTVTGDRILYRVAGEIRGNDTI+CFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRI
Sbjct: 372 EMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRI 431

Query: 427 GLAEIRCDLAGQKLGIGL 441
           GLAEIRCDLAGQKLG+GL
Sbjct: 432 GLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Sed0021222 vs. ExPASy TrEMBL
Match: A0A6J1J2F5 (aspartic proteinase PCS1 OS=Cucurbita maxima OX=3661 GN=LOC111482077 PE=3 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 1.1e-227
Identity = 395/439 (89.98%), Postives = 417/439 (94.99%), Query Frame = 0


Query: 6   TNNQFPKSCFLFF----FLFFQNLSLSSSINPAMVFPLKSQVIPQESVRRSPDKLPFRHN 65
           +N++F KS F FF    F  FQNL L SS+NPA++ PLK+QVIP ES+RRSPDKLPFRHN
Sbjct: 11  SNHKFLKSFFPFFLCILFSVFQNLILCSSLNPALLLPLKTQVIPPESIRRSPDKLPFRHN 70

Query: 66  ISLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVLSSSYAPIPCSSA 125
           +SLTV+LTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNP  SSSY PIPCSS+
Sbjct: 71  VSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPAGSSSYTPIPCSSS 130

Query: 126 TCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMDSI 185
           TCTDQTRDFPIPASCDSN LCHATLSYADASSSEG LA+DTFY+GNSGIS+VVFGCMDSI
Sbjct: 131 TCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGISNVVFGCMDSI 190

Query: 186 FSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLN 245
           FSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLN
Sbjct: 191 FSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLN 250

Query: 246 YTPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTF 305
           YTPLIE+STPLPYFDRVAYTVQLEGIKVS+KLLPIPESVFEPDHTGAGQTMVDSGTQFTF
Sbjct: 251 YTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQTMVDSGTQFTF 310

Query: 306 LLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFRG 365
           LLGPAYTALRDEFLN+TAGS+RV ED NFVFQGAMDLCYRVP NQTRLPPLP+VTLVFRG
Sbjct: 311 LLGPAYTALRDEFLNRTAGSIRVFEDSNFVFQGAMDLCYRVPMNQTRLPPLPSVTLVFRG 370

Query: 366 AEMTVTGDRILYRVAGEIRGNDTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSR 425
           AEMTVTGDRILYRV GEIRGND+IHCFTFGNSDLLGVEA+VIGHLHQQNVWMEFDLKKSR
Sbjct: 371 AEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKSR 430

Query: 426 IGLAEIRCDLAGQKLGIGL 441
           IGLAEIRCDLAGQKLG+GL
Sbjct: 431 IGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Sed0021222 vs. ExPASy TrEMBL
Match: A0A6J1CW88 (aspartic proteinase PCS1 OS=Momordica charantia OX=3673 GN=LOC111014974 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.0e-226
Identity = 394/443 (88.94%), Postives = 420/443 (94.81%), Query Frame = 0


Query: 5   YTNNQFPKSCFLFFFLF----FQNLSLSSSINPAMVFPLKSQVIPQESVRRSPDKLPFRH 64
           ++N+ F KSCF FF       FQN  L SS+NPA+V PLK+QVIP ESVRRSPDKLPFRH
Sbjct: 9   FSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRRSPDKLPFRH 68

Query: 65  NISLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSS-SSSTFNPVLSSSYAPIPCS 124
           N+SLTV+LTVGTPPQNVTMVIDTGSELSWLHCN+SQNSSS SSSTFNP+ SSSY+PIPCS
Sbjct: 69  NVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRSSSYSPIPCS 128

Query: 125 SATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMD 184
           S+TCTDQTRDFPIPASCDSNQLCHATLSYADASSSEG LA+DTFY+GNSGIS+VVFGCMD
Sbjct: 129 SSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGISNVVFGCMD 188

Query: 185 SIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSW--L 244
           SIFSSNNEED+KNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDAN+SW  L
Sbjct: 189 SIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANYSWLGL 248

Query: 245 APLNYTPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGT 304
           APLNYTPLI++STPLPYFDRVAYTV LEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGT
Sbjct: 249 APLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGT 308

Query: 305 QFTFLLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTL 364
           QFTFLLGPAYTALRDEFLNQTAGS+RVL+DPNFVFQGAMDLCYRVP NQ+RLPPLPAVTL
Sbjct: 309 QFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSRLPPLPAVTL 368

Query: 365 VFRGAEMTVTGDRILYRVAGEIRGNDTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDL 424
           VFRGAEMTV+GDRILYRV GEIRGND+IHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDL
Sbjct: 369 VFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDL 428

Query: 425 KKSRIGLAEIRCDLAGQKLGIGL 441
           +KSRIGLAEIRCDLAGQKLG+GL
Sbjct: 429 QKSRIGLAEIRCDLAGQKLGMGL 451

[Close]

BLAST of Sed0021222 vs. ExPASy TrEMBL
Match: A0A5D3DN23 (Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001570 PE=3 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 7.7e-226
Identity = 397/440 (90.23%), Postives = 416/440 (94.55%), Query Frame = 0


Query: 7   NNQFPKSCFLFFF--LF--FQNLSLSSSINPAMVFPLKSQVIPQESVRRSPDKLPFRHNI 66
           N +F KSCFLFFF  LF  F N+ L SS+NPA+V PLK+QVIP ESVRRSPDKLPFRHNI
Sbjct: 12  NIKFLKSCFLFFFCILFSVFYNIDLCSSVNPALVLPLKTQVIPPESVRRSPDKLPFRHNI 71

Query: 67  SLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQN--SSSSSSTFNPVLSSSYAPIPCSS 126
           SLTV+LTVGTPPQNVTMVIDTGSELSWLHCN SQN  SSSSSSTFNPV SSSY+PIPCSS
Sbjct: 72  SLTVSLTVGTPPQNVTMVIDTGSELSWLHCNTSQNSSSSSSSSTFNPVRSSSYSPIPCSS 131

Query: 127 ATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMDS 186
           +TCTDQTRDFPI  SCDSNQ CHATLSYADASSSEG LA+DTFY+GNSGI +VVFGCMDS
Sbjct: 132 STCTDQTRDFPIRPSCDSNQFCHATLSYADASSSEGNLATDTFYIGNSGIPNVVFGCMDS 191

Query: 187 IFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPL 246
           IFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPL
Sbjct: 192 IFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPL 251

Query: 247 NYTPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFT 306
           NYTPLIE+STPLPYFDRVAYTVQLEGIKV++KLLPIPESVFEPDHTGAGQTMVDSGTQFT
Sbjct: 252 NYTPLIEMSTPLPYFDRVAYTVQLEGIKVAHKLLPIPESVFEPDHTGAGQTMVDSGTQFT 311

Query: 307 FLLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFR 366
           FLLGPAYTALRD FLNQTAGSLR+ EDPNFVFQGAMDLCYRVPTNQTRLPPLP+VTLVFR
Sbjct: 312 FLLGPAYTALRDHFLNQTAGSLRLYEDPNFVFQGAMDLCYRVPTNQTRLPPLPSVTLVFR 371

Query: 367 GAEMTVTGDRILYRVAGEIRGNDTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKS 426
           GAEMTVTGDRILYRV GE RGND+IHCFTFGNSDLLGVEA+VIGHLHQQNVWMEFDLKKS
Sbjct: 372 GAEMTVTGDRILYRVPGERRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKS 431

Query: 427 RIGLAEIRCDLAGQKLGIGL 441
           RIGLAEIRCDLAGQKLG+GL
Sbjct: 432 RIGLAEIRCDLAGQKLGMGL 451

[Close]

BLAST of Sed0021222 vs. ExPASy TrEMBL
Match: A0A1S3B5W4 (aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103486376 PE=3 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 7.7e-226
Identity = 397/440 (90.23%), Postives = 416/440 (94.55%), Query Frame = 0


Query: 7   NNQFPKSCFLFFF--LF--FQNLSLSSSINPAMVFPLKSQVIPQESVRRSPDKLPFRHNI 66
           N +F KSCFLFFF  LF  F N+ L SS+NPA+V PLK+QVIP ESVRRSPDKLPFRHNI
Sbjct: 12  NIKFLKSCFLFFFCILFSVFYNIDLCSSVNPALVLPLKTQVIPPESVRRSPDKLPFRHNI 71

Query: 67  SLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQN--SSSSSSTFNPVLSSSYAPIPCSS 126
           SLTV+LTVGTPPQNVTMVIDTGSELSWLHCN SQN  SSSSSSTFNPV SSSY+PIPCSS
Sbjct: 72  SLTVSLTVGTPPQNVTMVIDTGSELSWLHCNTSQNSSSSSSSSTFNPVRSSSYSPIPCSS 131

Query: 127 ATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMDS 186
           +TCTDQTRDFPI  SCDSNQ CHATLSYADASSSEG LA+DTFY+GNSGI +VVFGCMDS
Sbjct: 132 STCTDQTRDFPIRPSCDSNQFCHATLSYADASSSEGNLATDTFYIGNSGIPNVVFGCMDS 191

Query: 187 IFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPL 246
           IFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPL
Sbjct: 192 IFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPL 251

Query: 247 NYTPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFT 306
           NYTPLIE+STPLPYFDRVAYTVQLEGIKV++KLLPIPESVFEPDHTGAGQTMVDSGTQFT
Sbjct: 252 NYTPLIEMSTPLPYFDRVAYTVQLEGIKVAHKLLPIPESVFEPDHTGAGQTMVDSGTQFT 311

Query: 307 FLLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLPAVTLVFR 366
           FLLGPAYTALRD FLNQTAGSLR+ EDPNFVFQGAMDLCYRVPTNQTRLPPLP+VTLVFR
Sbjct: 312 FLLGPAYTALRDHFLNQTAGSLRLYEDPNFVFQGAMDLCYRVPTNQTRLPPLPSVTLVFR 371

Query: 367 GAEMTVTGDRILYRVAGEIRGNDTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKS 426
           GAEMTVTGDRILYRV GE RGND+IHCFTFGNSDLLGVEA+VIGHLHQQNVWMEFDLKKS
Sbjct: 372 GAEMTVTGDRILYRVPGERRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKS 431

Query: 427 RIGLAEIRCDLAGQKLGIGL 441
           RIGLAEIRCDLAGQKLG+GL
Sbjct: 432 RIGLAEIRCDLAGQKLGMGL 451

[Close]

BLAST of Sed0021222 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 570.1 bits (1468), Expect = 1.6e-162
Identity = 285/417 (68.35%), Postives = 339/417 (81.29%), Query Frame = 0


Query: 28  SSSINPAMVFPLKSQVIPQESVRRSPDKLPFRHNISLTVALTVGTPPQNVTMVIDTGSEL 87
           SSS +  +V PLK+++ P +   R  DKL F HN++LTV LTVGTPPQN++MVIDTGSEL
Sbjct: 39  SSSSSQTLVLPLKTRITPTD--HRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSEL 98

Query: 88  SWLHCNRSQNSSSSSSTFNPVLSSSYAPIPCSSATCTDQTRDFPIPASCDSNQLCHATLS 147
           SWL CNRS N +  ++ F+P  SSSY+PIPCSS TC  +TRDF IPASCDS++LCHATLS
Sbjct: 99  SWLRCNRSSNPNPVNN-FDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLS 158

Query: 148 YADASSSEGILASDTFYLGNS-GISDVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFV 207
           YADASSSEG LA++ F+ GNS   S+++FGCM S+  S+ EED+K TGL+GMNRGSLSF+
Sbjct: 159 YADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFI 218

Query: 208 SQMGFPKFSYCIS-EYDFSGLLLLGDANFSWLAPLNYTPLIEISTPLPYFDRVAYTVQLE 267
           SQMGFPKFSYCIS   DF G LLLGD+NF+WL PLNYTPLI ISTPLPYFDRVAYTVQL 
Sbjct: 219 SQMGFPKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLT 278

Query: 268 GIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSLRVL 327
           GIKV+ KLLPIP+SV  PDHTGAGQTMVDSGTQFTFLLGP YTALR  FLN+T G L V 
Sbjct: 279 GIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVY 338

Query: 328 EDPNFVFQGAMDLCYRVPTNQTR---LPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGN 387
           EDP+FVFQG MDLCYR+   + R   L  LP V+LVF GAE+ V+G  +LYRV     GN
Sbjct: 339 EDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTVGN 398

Query: 388 DTIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGIG 440
           D+++CFTFGNSDL+G+EAYVIGH HQQN+W+EFDL++SRIGLA + CD++GQ+LGIG
Sbjct: 399 DSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

[Close]

BLAST of Sed0021222 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 540.8 bits (1392), Expect = 1.0e-153
Identity = 267/415 (64.34%), Postives = 327/415 (78.80%), Query Frame = 0


Query: 28  SSSINPAMVFPLKSQVIPQESVRRSPDKLPFRHNISLTVALTVGTPPQNVTMVIDTGSEL 87
           +SS N  ++F LK+Q +PQ     S DKL FRHN++LTV L VG PPQN++MV+DTGSEL
Sbjct: 33  TSSTNQTLLFSLKTQKLPQS----SSDKLSFRHNVTLTVTLAVGDPPQNISMVLDTGSEL 92

Query: 88  SWLHCNRSQNSSSSSSTFNPVLSSSYAPIPCSSATCTDQTRDFPIPASCD-SNQLCHATL 147
           SWLHC +S N     S FNPV SS+Y+P+PCSS  C  +TRD PIPASCD    LCH  +
Sbjct: 93  SWLHCKKSPN---LGSVFNPVSSSTYSPVPCSSPICRTRTRDLPIPASCDPKTHLCHVAI 152

Query: 148 SYADASSSEGILASDTFYLGNSGISDVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFV 207
           SYADA+S EG LA +TF +G+      +FGCMDS  SSN+EED+K+TGLMGMNRGSLSFV
Sbjct: 153 SYADATSIEGNLAHETFVIGSVTRPGTLFGCMDSGLSSNSEEDAKSTGLMGMNRGSLSFV 212

Query: 208 SQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLNYTPLIEISTPLPYFDRVAYTVQLEG 267
           +Q+GF KFSYCIS  D SG LLLGDA++SWL P+ YTPL+  STPLPYFDRVAYTVQLEG
Sbjct: 213 NQLGFSKFSYCISGSDSSGFLLLGDASYSWLGPIQYTPLVLQSTPLPYFDRVAYTVQLEG 272

Query: 268 IKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSLRVLE 327
           I+V +K+L +P+SVF PDHTGAGQTMVDSGTQFTFL+GP YTAL++EF+ QT   LR+++
Sbjct: 273 IRVGSKILSLPKSVFVPDHTGAGQTMVDSGTQFTFLMGPVYTALKNEFITQTKSVLRLVD 332

Query: 328 DPNFVFQGAMDLCYRV-PTNQTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGE-IRGNDT 387
           DP+FVFQG MDLCY+V  T +     LP V+L+FRGAEM+V+G ++LYRV G    G + 
Sbjct: 333 DPDFVFQGTMDLCYKVGSTTRPNFSGLPMVSLMFRGAEMSVSGQKLLYRVNGAGSEGKEE 392

Query: 388 IHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLA-EIRCDLAGQKLGI 439
           ++CFTFGNSDLLG+EA+VIGH HQQNVWMEFDL KSR+G A  +RCDLA Q+LG+
Sbjct: 393 VYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFAGNVRCDLASQRLGL 440

[Close]

BLAST of Sed0021222 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 260.4 bits (664), Expect = 2.6e-69
Identity = 162/446 (36.32%), Postives = 241/446 (54.04%), Query Frame = 0


Query: 11  PKSCFLFFFLFFQNLSLSSSIN-PAMVFPLKSQVIPQESV-----RRSPD--------KL 70
           PK  F FFFL + +LS S S++ P    P+ +             R++P         + 
Sbjct: 5   PKPLFFFFFLNYVSLSTSLSLHLPLTSLPISTTTNSHRFTTSLLSRKNPSPSSPPYNFRS 64

Query: 71  PFRHNISLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVLSSSYAPI 130
            F+++++L ++L +GTPPQ   MV+DTGS+LSW+ C+R +      ++F+P LSSS++ +
Sbjct: 65  RFKYSMALIISLPIGTPPQAQQMVLDTGSQLSWIQCHRKKLPPKPKTSFDPSLSSSFSTL 124

Query: 131 PCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGIS-DVVF 190
           PCS   C  +  DF +P SCDSN+LCH +  YAD + +EG L  +     N+ I+  ++ 
Sbjct: 125 PCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTEITPPLIL 184

Query: 191 GCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISE------YDFSGLLLL 250
           GC          E S + G++GMNRG LSFVSQ    KFSYCI        +  +G   L
Sbjct: 185 GCA--------TESSDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPGFTPTGSFYL 244

Query: 251 GD----ANFSWLAPLNYTPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDH 310
           GD      F +++ L +      S  +P  D +AYTV + GI+   K L I  SVF PD 
Sbjct: 245 GDNPNSHGFKYVSLLTFPE----SQRMPNLDPLAYTVPMIGIRFGLKKLNISGSVFRPDA 304

Query: 311 TGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTN 370
            G+GQTMVDSG++FT L+  AY  +R E + +    L+      +V+ G  D+C+    N
Sbjct: 305 GGSGQTMVDSGSEFTHLVDAAYDKVRAEIMTRVGRRLK----KGYVYGGTADMCF--DGN 364

Query: 371 QTRLPPL--PAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIHCFTFGNSDLLGVEAYVI 430
              +P L    V +  RG E+ V  +R+L  V G       IHC   G S +LG  + +I
Sbjct: 365 VAMIPRLIGDLVFVFTRGVEILVPKERVLVNVGG------GIHCVGIGRSSMLGAASNII 424

[Close]

BLAST of Sed0021222 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 240.7 bits (613), Expect = 2.2e-63
Identity = 162/457 (35.45%), Postives = 243/457 (53.17%), Query Frame = 0


Query: 10  FPKSCFLFFFLFFQNLSLSSSINPAMVFPLKS-QVIPQES---------VRRSP----DK 69
           F K  ++FFF F  ++SLS S + ++ FPL S ++ P  +          RR+P      
Sbjct: 9   FLKLLYIFFF-FCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPPSSP 68

Query: 70  LPFRHNI----SLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQNSS---SSSSTFNPV 129
             FR NI    +L ++L +GTP Q+  +V+DTGS+LSW+ C+  +        +++F+P 
Sbjct: 69  YTFRSNIKYSMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPPPTTSFDPS 128

Query: 130 LSSSYAPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNS 189
           LSSS++ +PCS   C  +  DF +P SCDSN+LCH +  YAD + +EG L  + F   NS
Sbjct: 129 LSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSNS 188

Query: 190 GIS-DVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYD----- 249
             +  ++ GC         +E +   G++GMN G LSF+SQ    KFSYCI         
Sbjct: 189 QTTPPLILGCA--------KESTDEKGILGMNLGRLSFISQAKISKFSYCIPTRSNRPGL 248

Query: 250 -FSGLLLLGD----ANFSWLAPLNYTPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIP 309
             +G   LGD      F +++ L +      S  +P  D +AYTV L+GI++  K L IP
Sbjct: 249 ASTGSFYLGDNPNSRGFKYVSLLTFPQ----SQRMPNLDPLAYTVPLQGIRIGQKRLNIP 308

Query: 310 ESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMD 369
            SVF PD  G+GQTMVDSG++FT L+  AY  +++E +      L+      +V+    D
Sbjct: 309 GSVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIVRLVGSRLK----KGYVYGSTAD 368

Query: 370 LCYRVPTNQTRLPPLPAVTLVF---RGAEMTVTGDRILYRVAGEIRGNDTIHCFTFGNSD 429
           +C+    N +         LVF   RG E+ V    +L  V G       IHC   G S 
Sbjct: 369 MCF--DGNHSMEIGRLIGDLVFEFGRGVEILVEKQSLLVNVGG------GIHCVGIGRSS 428

Query: 430 LLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDL 432
           +LG  + +IG++HQQN+W+EFD+   R+G ++  C L
Sbjct: 429 MLGAASNIIGNVHQQNLWVEFDVTNRRVGFSKAECRL 440

[Close]

BLAST of Sed0021222 vs. TAIR 10
Match: AT1G09750.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 144.8 bits (364), Expect = 1.6e-34
Identity = 119/378 (31.48%), Postives = 172/378 (45.50%), Query Frame = 0


Query: 60  HNISLTVALTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVLSSSYAPIPCS 119
           H  +  V   +GTPPQ + MV+DT ++  WL C+     S++S++FN   SS+Y+ + CS
Sbjct: 100 HIGNYVVRAKLGTPPQLMFMVLDTSNDAVWLPCSGCSGCSNASTSFNTNSSSTYSTVSCS 159

Query: 120 SATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGILASDTFYLGNSGISDVVFGCMD 179
           +A CT Q R    P+S     +C    SY   SS    L  DT  L    I +  FGC++
Sbjct: 160 TAQCT-QARGLTCPSSSPQPSVCSFNQSYGGDSSFSASLVQDTLTLAPDVIPNFSFGCIN 219

Query: 180 SIFSSNNEEDSKNTGLMGMNRGSLSFVSQ---MGFPKFSYCISEYD---FSGLLLLGDAN 239
           S  +S N    +  GLMG+ RG +S VSQ   +    FSYC+  +    FSG L LG   
Sbjct: 220 S--ASGNSLPPQ--GLMGLGRGPMSLVSQTTSLYSGVFSYCLPSFRSFYFSGSLKLG--L 279

Query: 240 FSWLAPLNYTPLIEISTPLPYFDRVAYTVQLEGIKVSNKLLPIPESVFEPDHTGAGQTMV 299
                 + YTPL+  +   P      Y V L G+ V +  +P+       D      T++
Sbjct: 280 LGQPKSIRYTPLLR-NPRRPSL----YYVNLTGVSVGSVQVPVDPVYLTFDANSGAGTII 339

Query: 300 DSGTQFTFLLGPAYTALRDEFLNQTAGSLRVLEDPNFVFQGAMDLCYRVPTNQTRLPPLP 359
           DSGT  T    P Y A+RDEF  Q   S       +F   GA D C+            P
Sbjct: 340 DSGTVITRFAQPVYEAIRDEFRKQVNVS-------SFSTLGAFDTCFSADNENV----AP 399

Query: 360 AVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIHCFTF-GNSDLLGVEAYVIGHLHQQNVW 419
            +TL     ++ +  +  L   +       T+ C +  G          VI +L QQN+ 
Sbjct: 400 KITLHMTSLDLKLPMENTLIHSSA-----GTLTCLSMAGIRQNANAVLNVIANLQQQNLR 449

Query: 420 MEFDLKKSRIGLAEIRCD 431
           + FD+  SRIG+A   C+
Sbjct: 460 ILFDVPNSRIGIAPEPCN 449

The following BLAST results are available for this feature:

BLAST of Sed0021222 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948372.1	2.2e-227	90.41	aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022948381.1 aspartic prot...	[more]
XP_038905417.1	2.2e-227	90.43	aspartic proteinase PCS1 [Benincasa hispida]	[more]
XP_022983486.1	2.2e-227	89.98	aspartic proteinase PCS1 [Cucurbita maxima]	[more]
XP_022145548.1	4.2e-226	88.94	aspartic proteinase PCS1 [Momordica charantia]	[more]
XP_008442528.1	1.6e-225	90.23	PREDICTED: aspartic proteinase PCS1 [Cucumis melo] >KAA0044101.1 aspartic protei...	[more]

BLAST of Sed0021222 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZL3	2.2e-161	68.35	Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1	[more]
Q766C3	2.0e-37	32.07	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	4.9e-36	29.04	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
O04496	2.3e-33	31.48	Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1	[more]
Q6F4N5	4.3e-32	31.95	Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1	[more]

BLAST of Sed0021222 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G928	1.1e-227	90.41	aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111452070 PE=3...	[more]
A0A6J1J2F5	1.1e-227	89.98	aspartic proteinase PCS1 OS=Cucurbita maxima OX=3661 GN=LOC111482077 PE=3 SV=1	[more]
A0A6J1CW88	2.0e-226	88.94	aspartic proteinase PCS1 OS=Momordica charantia OX=3673 GN=LOC111014974 PE=3 SV=...	[more]
A0A5D3DN23	7.7e-226	90.23	Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3B5W4	7.7e-226	90.23	aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103486376 PE=3 SV=1	[more]

BLAST of Sed0021222 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G02190.1	1.6e-162	68.35	Eukaryotic aspartyl protease family protein	[more]
AT2G39710.1	1.0e-153	64.34	Eukaryotic aspartyl protease family protein	[more]
AT1G66180.1	2.6e-69	36.32	Eukaryotic aspartyl protease family protein	[more]
AT5G37540.1	2.2e-63	35.45	Eukaryotic aspartyl protease family protein	[more]
AT1G09750.1	1.6e-34	31.48	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 70..90 score: 46.19 coord: 291..302 score: 32.91 coord: 401..416 score: 18.07
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR47965	ASPARTYL PROTEASE-RELATED	coord: 23..438
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 66..231 e-value: 2.4E-38 score: 132.1
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 235..432 e-value: 4.9E-44 score: 152.1
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 57..231 e-value: 3.8E-36 score: 126.8
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 65..429
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 259..424 e-value: 4.8E-37 score: 127.2
None	No IPR available	PANTHER	PTHR47965:SF64	ASPARTIC PROTEINASE PCS1	coord: 23..438
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 79..90
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 64..425 score: 35.45871
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 65..429 e-value: 9.77437E-71 score: 222.523

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0021222.1	Sed0021222.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046274	lignin catabolic process
biological_process	GO:0012501	programmed cell death
biological_process	GO:0006508	proteolysis
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0052716	hydroquinone:oxygen oxidoreductase activity