Sed0020943 (gene) Chayote v1

Overview
NameSed0020943
Typegene
OrganismSechium edule (Chayote v1)
DescriptionHeat shock protein 70 family
LocationLG12: 5737452 .. 5740479 (+)
RNA-Seq ExpressionSed0020943
SyntenySed0020943
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTCTGGGAAGCTCTAGAAGAATGACGATGAAGCTTTCTATCAATGGAATCTCTACAACTTTCCACTTCTTTCTTCACCGACTCATTTCATAGCTTATAACCATACTCCCATTTCTCCCATCGCTTGTTTCTGCAATCTGCTTCTGCTTGCCTCAACCATTATTAAGATCGCTTCTTCTTTCGGTGATTCTTTCTCTTTTTTTTTTTTTTTTTTAATTAATCAATTTGGTCGCTTGCAGTTCTGGGTATGTCTTTTTCTTCGATTTGGTTCCTGTTAGTCAGCATTTTCCATTCTGTTTGTGCTTTTTCTATAATGGGTTTGATGTATTGTGTGATTTTTCTTTGCCTAATTGCGGGATTTAATGCAGTGGTAGTTTGAATTCTTGCGGCTTTGTTGCTTTTTAATGTGATTTCTTTTGCTGCATCTCTTCTGCTGATGTAGAGAAATGGTGTTTGGTATTGCTCGGAAGGTGTTTGTGAAAATGAATCTTCGGGAAAATGAATATTTTGTTTCAATGTGTATGCTGTTATTTGAAATTTTATAATTGTAATGAGAATTTGAGTAGAGAGATGGATTATTGTTATTTGAATTTTTATATAATTCCTATTGTGGCATGAAAACCTGTCTATCTGAAAGGAATGTTGGTTTTCAACAAGCATATCTGCTTGGGGAGTTTTGTTGTTCTTCTGGCCCTATTATTCTGTGTTATTCATTGCAGTTATGGTGTAAGAAAAAAGAATCGCTATCCTATTGCCAAAACTAAGGCCCAAGTTCACACATTATATGGTTTATGCTGGTCGTCAATTGATATTATATGAGTTTCCTTAACATTCAAATGTTGTAATGCTAAGTGGTGAAATAGTTGAGTTACATATACTGATTAAAGAAAAAAAAATCTGATTCTATCTGATTTTGTCTCTAAAGGTTCTCCTCTTTTGTCCGTTATAGCTAGATCGAGTATAGAAACATTTTGTTCTTGTTTATTCCTTTGGAAGTGCTGGAAAGATGGTTCAGTGGATTGTAAATCCTTAAGTAGAGTAGTAGATTATCTATTCTATATCCTCTAGTGCGATCTATAATTAGTAATAACAAGGCCTTTGATGAATTACTCTCTGAAAGCTAGAGCTTTATATTGATCAAAATGCAGATTATGGCTGAACCAATATACACTGTAGCTTCTGATAGTGAAACCACAGGGGAGGAAAAAGCAACCTCAACCTTTCCAGAGATTGTGGTTGGAATTGATATTGGTACTTCACAATGTAGTATTGCAGTCTGGAATGGATCTCAGGTAGAGCTTCTTAAGAACACTAGAAATCAGAAAATTATGAGATCATATGTCACCTTCAAAGACGAAACTCCTTCGGGAGGAGTTAGTAATGAACTTAGCCATGAGTATGACATGCTCTCCGGTGCGGCTATTTTCAATATGAAACGTTTGATTGGAAGAGTTGACACAGACCCTGTCGTCCATGCAAGCAAAAGCCTTCCTTTTCTAGTGCAAACTTTGGACATTGGTGTTCGCCCGTTCGTTGCAGCTTTAGTGAATAATGTATGGAGATCTACCACTCCTGAGGAAGTTCTTGCAATCTTTCTTGTTGAACTAAAAATGATGGCTGAATATCAACTAAAGCGGCCAATTAGAAATATTGTTTTGACGATTCCAGTTTCATTCAGCCGGTTCCAGCTTACTCGGGTTGAACGAGCTTGTGCAATGGCTGGCCTTCAAGCGAGGTTGATGCCAGAACCAACAGCGGTAGCTTTATTATATGCCCAACAACAACAGCAGGCCGCACACGAGAACATGGGCAGTGGAAGTGAAAAAATTGTGCTTATTTTTAACATGGGTGCTGGGTATTGTGATGTTGCTGTATCAGCTACAGGCGGGGGAGTGTCACAGATCAGATCCCTGGCTGGAAGTCCTATTGGAGGTGAAGATCTACTTCAAAACACGATGAGGTATCTGTTGCCAAATTCTGAATCTATATTCTCTCACCGTGGAATCAATGACATCAGAACGATGGGATTGCTCCGAGTTGCCACCCAGGACGCTATCCACGAGCTTTCATTCCAGTCTAGTGTTCAAATTGATGTGGACTTGGGGAATGGTTTAAAAATATGCAAGGTCCTAAGCCGAGACGAGTTTGAGATGGTGAACAGCAAGGTGTTCGAGAAATGTAAGAACCTCGTAAAGCAATGCTTACACGATGCAAGAGTAGAAATTGAAGATGTGAGCGATGTGGTAGTCGTTGGTGGATGTTCATACATGCCAAGAATCAGAAAACTTCTGATGGAAATAACTAAAAAGAAGGTACTTTATGAAGGAATGGATCCTTTAGAAGCTGCTGTTTCTGGTGCAGCTTTAGAAGGTGCGGTGGCGTCAGGCATTGGCGACCCATTTGGAAATTTAGATCTTCTTAGCATCCAAGCGACGCCTCTCGCCATTGGAATTCGGGCGGATGGAAACAGCTTCATTCCAATTATTCCCAAGAACACAACAATGCCTGCCCGCAAGGAGTTGGCTTTCACAACTGTTCAAGATAATCAAAGCGAGGCACTGATCGTTGTCTACGAAGGTGAAGAGACAAGAGCAGAAGAAAACCATCTGCTTGGCTATTTCAAGATCATTGGAATTCCACCAGCACCCAAGGGAGTCCCTGAAATTACTATTGGTATGGACATTGACTCAACAAATATGCTTAGAGTTCTAGCTGGAGCCACACTCCCAGGTGCTCAACACCCCGCAACTCCTTTCATGGAAGTCAAAATGCCGACCGTCGACGATGGACATGGCTGGTGTGCGGAAGCTCTGCATAGAAAATATGGCACAGCCGTGGACTTGGTTACCGTGCAGAAGAAAATCTAGGGAACATATTTAGATTGTGATGCTCTGTTTTCGTGTTACACTGTCATATGACTCATAAGTTCAATACTAGTACATAGGGAAATAATGTACGGTGATAATGTATATTAATGTTTTAAGTTGTTGATATCATCCTGAAGATTTTGCTCTTGACATTGGCGAT

mRNA sequence

TTCTCTGGGAAGCTCTAGAAGAATGACGATGAAGCTTTCTATCAATGGAATCTCTACAACTTTCCACTTCTTTCTTCACCGACTCATTTCATAGCTTATAACCATACTCCCATTTCTCCCATCGCTTGTTTCTGCAATCTGCTTCTGCTTGCCTCAACCATTATTAAGATCGCTTCTTCTTTCGATTATGGCTGAACCAATATACACTGTAGCTTCTGATAGTGAAACCACAGGGGAGGAAAAAGCAACCTCAACCTTTCCAGAGATTGTGGTTGGAATTGATATTGGTACTTCACAATGTAGTATTGCAGTCTGGAATGGATCTCAGGTAGAGCTTCTTAAGAACACTAGAAATCAGAAAATTATGAGATCATATGTCACCTTCAAAGACGAAACTCCTTCGGGAGGAGTTAGTAATGAACTTAGCCATGAGTATGACATGCTCTCCGGTGCGGCTATTTTCAATATGAAACGTTTGATTGGAAGAGTTGACACAGACCCTGTCGTCCATGCAAGCAAAAGCCTTCCTTTTCTAGTGCAAACTTTGGACATTGGTGTTCGCCCGTTCGTTGCAGCTTTAGTGAATAATGTATGGAGATCTACCACTCCTGAGGAAGTTCTTGCAATCTTTCTTGTTGAACTAAAAATGATGGCTGAATATCAACTAAAGCGGCCAATTAGAAATATTGTTTTGACGATTCCAGTTTCATTCAGCCGGTTCCAGCTTACTCGGGTTGAACGAGCTTGTGCAATGGCTGGCCTTCAAGCGAGGTTGATGCCAGAACCAACAGCGGTAGCTTTATTATATGCCCAACAACAACAGCAGGCCGCACACGAGAACATGGGCAGTGGAAGTGAAAAAATTGTGCTTATTTTTAACATGGGTGCTGGGTATTGTGATGTTGCTGTATCAGCTACAGGCGGGGGAGTGTCACAGATCAGATCCCTGGCTGGAAGTCCTATTGGAGGTGAAGATCTACTTCAAAACACGATGAGGTATCTGTTGCCAAATTCTGAATCTATATTCTCTCACCGTGGAATCAATGACATCAGAACGATGGGATTGCTCCGAGTTGCCACCCAGGACGCTATCCACGAGCTTTCATTCCAGTCTAGTGTTCAAATTGATGTGGACTTGGGGAATGGTTTAAAAATATGCAAGGTCCTAAGCCGAGACGAGTTTGAGATGGTGAACAGCAAGGTGTTCGAGAAATGTAAGAACCTCGTAAAGCAATGCTTACACGATGCAAGAGTAGAAATTGAAGATGTGAGCGATGTGGTAGTCGTTGGTGGATGTTCATACATGCCAAGAATCAGAAAACTTCTGATGGAAATAACTAAAAAGAAGGTACTTTATGAAGGAATGGATCCTTTAGAAGCTGCTGTTTCTGGTGCAGCTTTAGAAGGTGCGGTGGCGTCAGGCATTGGCGACCCATTTGGAAATTTAGATCTTCTTAGCATCCAAGCGACGCCTCTCGCCATTGGAATTCGGGCGGATGGAAACAGCTTCATTCCAATTATTCCCAAGAACACAACAATGCCTGCCCGCAAGGAGTTGGCTTTCACAACTGTTCAAGATAATCAAAGCGAGGCACTGATCGTTGTCTACGAAGGTGAAGAGACAAGAGCAGAAGAAAACCATCTGCTTGGCTATTTCAAGATCATTGGAATTCCACCAGCACCCAAGGGAGTCCCTGAAATTACTATTGGTATGGACATTGACTCAACAAATATGCTTAGAGTTCTAGCTGGAGCCACACTCCCAGGTGCTCAACACCCCGCAACTCCTTTCATGGAAGTCAAAATGCCGACCGTCGACGATGGACATGGCTGGTGTGCGGAAGCTCTGCATAGAAAATATGGCACAGCCGTGGACTTGGTTACCGTGCAGAAGAAAATCTAGGGAACATATTTAGATTGTGATGCTCTGTTTTCGTGTTACACTGTCATATGACTCATAAGTTCAATACTAGTACATAGGGAAATAATGTACGGTGATAATGTATATTAATGTTTTAAGTTGTTGATATCATCCTGAAGATTTTGCTCTTGACATTGGCGAT

Coding sequence (CDS)

ATGGCTGAACCAATATACACTGTAGCTTCTGATAGTGAAACCACAGGGGAGGAAAAAGCAACCTCAACCTTTCCAGAGATTGTGGTTGGAATTGATATTGGTACTTCACAATGTAGTATTGCAGTCTGGAATGGATCTCAGGTAGAGCTTCTTAAGAACACTAGAAATCAGAAAATTATGAGATCATATGTCACCTTCAAAGACGAAACTCCTTCGGGAGGAGTTAGTAATGAACTTAGCCATGAGTATGACATGCTCTCCGGTGCGGCTATTTTCAATATGAAACGTTTGATTGGAAGAGTTGACACAGACCCTGTCGTCCATGCAAGCAAAAGCCTTCCTTTTCTAGTGCAAACTTTGGACATTGGTGTTCGCCCGTTCGTTGCAGCTTTAGTGAATAATGTATGGAGATCTACCACTCCTGAGGAAGTTCTTGCAATCTTTCTTGTTGAACTAAAAATGATGGCTGAATATCAACTAAAGCGGCCAATTAGAAATATTGTTTTGACGATTCCAGTTTCATTCAGCCGGTTCCAGCTTACTCGGGTTGAACGAGCTTGTGCAATGGCTGGCCTTCAAGCGAGGTTGATGCCAGAACCAACAGCGGTAGCTTTATTATATGCCCAACAACAACAGCAGGCCGCACACGAGAACATGGGCAGTGGAAGTGAAAAAATTGTGCTTATTTTTAACATGGGTGCTGGGTATTGTGATGTTGCTGTATCAGCTACAGGCGGGGGAGTGTCACAGATCAGATCCCTGGCTGGAAGTCCTATTGGAGGTGAAGATCTACTTCAAAACACGATGAGGTATCTGTTGCCAAATTCTGAATCTATATTCTCTCACCGTGGAATCAATGACATCAGAACGATGGGATTGCTCCGAGTTGCCACCCAGGACGCTATCCACGAGCTTTCATTCCAGTCTAGTGTTCAAATTGATGTGGACTTGGGGAATGGTTTAAAAATATGCAAGGTCCTAAGCCGAGACGAGTTTGAGATGGTGAACAGCAAGGTGTTCGAGAAATGTAAGAACCTCGTAAAGCAATGCTTACACGATGCAAGAGTAGAAATTGAAGATGTGAGCGATGTGGTAGTCGTTGGTGGATGTTCATACATGCCAAGAATCAGAAAACTTCTGATGGAAATAACTAAAAAGAAGGTACTTTATGAAGGAATGGATCCTTTAGAAGCTGCTGTTTCTGGTGCAGCTTTAGAAGGTGCGGTGGCGTCAGGCATTGGCGACCCATTTGGAAATTTAGATCTTCTTAGCATCCAAGCGACGCCTCTCGCCATTGGAATTCGGGCGGATGGAAACAGCTTCATTCCAATTATTCCCAAGAACACAACAATGCCTGCCCGCAAGGAGTTGGCTTTCACAACTGTTCAAGATAATCAAAGCGAGGCACTGATCGTTGTCTACGAAGGTGAAGAGACAAGAGCAGAAGAAAACCATCTGCTTGGCTATTTCAAGATCATTGGAATTCCACCAGCACCCAAGGGAGTCCCTGAAATTACTATTGGTATGGACATTGACTCAACAAATATGCTTAGAGTTCTAGCTGGAGCCACACTCCCAGGTGCTCAACACCCCGCAACTCCTTTCATGGAAGTCAAAATGCCGACCGTCGACGATGGACATGGCTGGTGTGCGGAAGCTCTGCATAGAAAATATGGCACAGCCGTGGACTTGGTTACCGTGCAGAAGAAAATCTAG

Protein sequence

MAEPIYTVASDSETTGEEKATSTFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDETPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQLTRVERACAMAGLQARLMPEPTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVAVSATGGGVSQIRSLAGSPIGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVATQDAIHELSFQSSVQIDVDLGNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIEDVSDVVVVGGCSYMPRIRKLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPATPFMEVKMPTVDDGHGWCAEALHRKYGTAVDLVTVQKKI
Homology
BLAST of Sed0020943 vs. NCBI nr
Match: XP_004134140.1 (heat shock 70 kDa protein 8 [Cucumis sativus] >XP_011650982.1 heat shock 70 kDa protein 8 [Cucumis sativus] >KGN56991.1 hypothetical protein Csa_011014 [Cucumis sativus])

HSP 1 Score: 1040.0 bits (2688), Expect = 7.4e-300
Identity = 519/570 (91.05%), Postives = 552/570 (96.84%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKATSTFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60
           MAEPIYTVASDSETTGE++ TSTFPEI VGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDETPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKD+TPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRN+VLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQARLMPEPTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVA 240
           TRVERACAMAGLQ RLMPEPTAVALLYAQQQQQA H++MGSGSEKI LIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSATGGGVSQIRSLAGSPIGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVATQD 300
           VSA GGGVSQIR+LAGSPIGGEDLLQNTM+YLLPNSESIFSHRGI++IR MGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHELSFQSSVQIDVDLGNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIED 360
           AIH+LSFQSSV+I+V LGNG K+CKVLSR++FEMVNSKVFEKC+NLVKQCLHDAR+ IED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARLGIED 360

Query: 361 VSDVVVVGGCSYMPRIRKLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNL 420
           +SDVVVVGGCSY+P+IR LLMEI+KKK LYEG+DPLEAAVSGAALEGA+ASGIGDPFG+L
Sbjct: 361 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKEL FTT+QDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITI MDIDS+NMLRVLAGATLPGAQHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHRKYGTAVDLVTVQKK 571
           PTVDDGHGWCAEALH KYG  ++LVT++KK
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKK 570

BLAST of Sed0020943 vs. NCBI nr
Match: KAA0049367.1 (heat shock 70 kDa protein 8 [Cucumis melo var. makuwa] >TYK17192.1 heat shock 70 kDa protein 8 [Cucumis melo var. makuwa])

HSP 1 Score: 1038.5 bits (2684), Expect = 2.2e-299
Identity = 520/570 (91.23%), Postives = 551/570 (96.67%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKATSTFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60
           MAEPIYTVASDSETTGE++ TSTFPEI VGIDIGTSQCSIAVWNGS VELLKNTR+QKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 61  RSYVTFKDETPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKD+TPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRN+VLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQARLMPEPTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVA 240
           TRVERACAMAG Q RLMPEPTAVALLYAQQQQQAAH++MGSGSEKI LIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSATGGGVSQIRSLAGSPIGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVATQD 300
           VSA GGGVSQIR+LAGSPIGGEDLLQNTM+YLLPNSESIFSHRGI++IR MGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHELSFQSSVQIDVDLGNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIED 360
           AIH+LSFQSSV+I+V LGNG K+CKVLSR+EFEMVNSKVFEKC+NLVKQCLHDARV IED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 361 VSDVVVVGGCSYMPRIRKLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNL 420
           +SDVVVVGGCSY+P+IRKLLMEI+KKK LYEG+DPLEAAVSGAALEGA+ASGIGDPFG+L
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKEL FTTVQDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITI MDIDS+NMLRVLAGA LPGAQHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHRKYGTAVDLVTVQKK 571
           PTVDDGHGWCAEALH KYG  ++LVT++KK
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKK 570

BLAST of Sed0020943 vs. NCBI nr
Match: XP_008438682.1 (PREDICTED: heat shock 70 kDa protein 8 [Cucumis melo])

HSP 1 Score: 1036.9 bits (2680), Expect = 6.3e-299
Identity = 519/570 (91.05%), Postives = 550/570 (96.49%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKATSTFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60
           MAEPIYTVASDSETTGE++ TSTFPEI VGIDIGTSQCSIAVWNGS VELLKNTR+QKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 61  RSYVTFKDETPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKD+TPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRN+VLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQARLMPEPTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVA 240
           TRVERACAMAG Q RLMPEPTAVALLYAQQQQQAAH++MGSGSEKI LIFNMGAGYCD A
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240

Query: 241 VSATGGGVSQIRSLAGSPIGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVATQD 300
           VSA GGGVSQIR+LAGSPIGGEDLLQNTM+YLLPNSESIFSHRGI++IR MGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHELSFQSSVQIDVDLGNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIED 360
           AIH+LSFQSSV+I+V LGNG K+CKVLSR+EFEMVNSKVFEKC+NLVKQCLHDARV IED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 361 VSDVVVVGGCSYMPRIRKLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNL 420
           +SDVVVVGGCSY+P+IRKLLMEI+KKK LYEG+DPLEAAVSGAALEGA+ASGIGDPFG+L
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKEL FTTVQDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITI MDIDS+NMLRVLAGA LPGAQHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHRKYGTAVDLVTVQKK 571
           PTVDDGHGWCAEALH KYG  ++LVT++KK
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKK 570

BLAST of Sed0020943 vs. NCBI nr
Match: XP_038897373.1 (heat shock 70 kDa protein 8 [Benincasa hispida])

HSP 1 Score: 1036.2 bits (2678), Expect = 1.1e-298
Identity = 519/570 (91.05%), Postives = 550/570 (96.49%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKATSTFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60
           MAEPIYTVASDSETTGE+K TSTFP+I VGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM
Sbjct: 1   MAEPIYTVASDSETTGEDKTTSTFPDIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDETPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKD+TPSGGVSNELSHE+DMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEFDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKR I+N+VLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRSIKNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQARLMPEPTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVA 240
           TRVERACAMAGLQ RLMPEPTAVALLYAQQQQQA HE+MGSGSEKI LIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHESMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSATGGGVSQIRSLAGSPIGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVATQD 300
           VSA GGGVSQIR+LAGSPIGGEDLLQNTM+YLLPNSESIFSHRGI++IR MGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHELSFQSSVQIDVDLGNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIED 360
           AIH+LSFQSSVQIDV+LGNG K+CKVLSR+EFEMVNSKVFEKC+NLVKQCLHDARV IED
Sbjct: 301 AIHKLSFQSSVQIDVNLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVRIED 360

Query: 361 VSDVVVVGGCSYMPRIRKLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNL 420
           +SDVVVVGGCSY+P+IR LLMEI K K LYEGMDPLEAAVSGAALEGA+ASGIGDPFG+L
Sbjct: 361 LSDVVVVGGCSYIPKIRNLLMEICKNKALYEGMDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKEL FTTV+DNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVRDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPATPFMEVKM 540
           AEENHLLGY KIIGIPPAPKGVPEITI MDIDS+NMLRVLAGATLPG+QHPATP+MEVKM
Sbjct: 481 AEENHLLGYVKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHRKYGTAVDLVTVQKK 571
           PTVDDGHGWCAEALH KYG  ++LV+++KK
Sbjct: 541 PTVDDGHGWCAEALHGKYGPTMELVSLRKK 570

BLAST of Sed0020943 vs. NCBI nr
Match: XP_022138266.1 (heat shock 70 kDa protein 8 [Momordica charantia])

HSP 1 Score: 1023.8 bits (2646), Expect = 5.5e-295
Identity = 514/570 (90.18%), Postives = 545/570 (95.61%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKATSTFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60
           MAEPIYTVASDSETTGEEK TSTFPEI VGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM
Sbjct: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDETPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKD+TPSGGVS+EL HEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSSELCHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQL 180
           DIGVRPFVAALVNN+WRSTTPEEVLAIFLVELKMMAEYQLKRPIRN+VLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNMWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQARLMPEPTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVA 240
           TRVERACAMAGLQ RLMPEPTAVALLYAQQQ QA HENMGSGSEKI L+FNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQHQAVHENMGSGSEKIALVFNMGAGYCDVA 240

Query: 241 VSATGGGVSQIRSLAGSPIGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVATQD 300
           +SAT GGVSQIR+L GSPIGGEDLLQNTM+YLLPNSESIFS+ GI++IR MGLLRVATQD
Sbjct: 241 ISATAGGVSQIRALTGSPIGGEDLLQNTMKYLLPNSESIFSNHGIDEIRRMGLLRVATQD 300

Query: 301 AIHELSFQSSVQIDVDLGNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIED 360
           AI +LSFQ+S QIDV+LGNG KICKVLSR+EFEMVNSKVFEKC+NLVKQCLHDARVEIED
Sbjct: 301 AILKLSFQTSAQIDVNLGNGSKICKVLSREEFEMVNSKVFEKCENLVKQCLHDARVEIED 360

Query: 361 VSDVVVVGGCSYMPRIRKLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNL 420
           +SDVVVVGGCS +P+IRKLLMEI+KKK LYE +DPLEAAVSGAA+EGAVASGIGDPFG+L
Sbjct: 361 LSDVVVVGGCSSIPKIRKLLMEISKKKALYEEIDPLEAAVSGAAIEGAVASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQ TPLAIGIRADGN+FIPIIPKNTTMPARKEL FTTVQDNQSEALIVVYEGEE R
Sbjct: 421 DLLSIQVTPLAIGIRADGNNFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEEAR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITI MDIDS+N LRVLAGATLPGAQHPATPFMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNTLRVLAGATLPGAQHPATPFMEVKM 540

Query: 541 PTVDDGHGWCAEALHRKYGTAVDLVTVQKK 571
           PT+DDGH WCAEALH K+G ++DLVT++KK
Sbjct: 541 PTIDDGHSWCAEALHGKHGVSMDLVTLRKK 570

BLAST of Sed0020943 vs. ExPASy Swiss-Prot
Match: Q9SKY8 (Heat shock 70 kDa protein 8 OS=Arabidopsis thaliana OX=3702 GN=HSP70-8 PE=1 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 5.2e-240
Identity = 406/574 (70.73%), Postives = 488/574 (85.02%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKATS--TFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQK 60
           MAE  YTVASDSE TGEEK++S  + PEI +GIDIGTSQCSIAVWNGSQV +L+NTRNQK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 61  IMRSYVTFKDETPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQ 120
           +++S+VTFKDE P+GGVSN+L+HE +ML+GAAIFNMKRL+GRVDTDPVVHASK+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 121 TLDIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRF 180
           TLDIGVRPF+AALVNN WRSTTPEEVLAIFLVEL++MAE QLKRP+RN+VLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 181 QLTRVERACAMAGLQA-RLMPEPTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYC 240
           QLTR ERACAMAGL   RLMPEPTA+ALLYAQQQQ   H+NMGSGSE++ +IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 241 DVAVSATGGGVSQIRSLAGSPIGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVA 300
           DVAV+AT GGVSQI++LAGSPIGGED+LQNT+R++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 301 TQDAIHELSFQSSVQIDVDLGNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVE 360
            QDAIH L+ Q +VQI+VDLGNG KI KVL R EFE VN KVFE+C+ LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 361 IEDVSDVVVVGGCSYMPRIRKLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPF 420
             D+ D+++VGGCSY+P++R ++  + KK  +Y+G++PLEAAV GAALEGAV SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 421 GNLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGE 480
           G+LDLL+IQATPLA+G+RA+GN FIP+IP+NT +PARK+L FTTVQDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 481 ETRAEENHLLGYFKIIGIPPAPKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPATPFME 540
               EENHLLGYFK++GIPPAPKGVPEI + MDID++N LRV A   +PG+  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 541 VKMPTVDDGHGWCAEALHRKYGTAVDLVTVQKKI 572
           V+MPTVDDGHGWCA+AL+ KYG  +DL+T+Q+K+
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRKM 563

BLAST of Sed0020943 vs. ExPASy Swiss-Prot
Match: Q9LHA8 (Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 2.4e-75
Identity = 176/513 (34.31%), Postives = 285/513 (55.56%), Query Frame = 0

Query: 29  VGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDETPSGGVSNELSHEYDMLSG 88
           +GID+GT+   + VW   +VE++ N +  +   SYV F D      + +   ++  M   
Sbjct: 10  IGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNPT 69

Query: 89  AAIFNMKRLIGRVDTDPVVHASKS-LPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLAI 148
             +F+ KRLIGR  +DP V A KS  PF V +   G +P +        +  + EE+ ++
Sbjct: 70  NTVFDAKRLIGRRYSDPSVQADKSHWPFKVVS-GPGEKPMIVVNHKGEEKQFSAEEISSM 129

Query: 149 FLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQLTRVERACAMAGLQA-RLMPEPTAVALL 208
            L++++ +AE  L  P++N V+T+P  F+  Q    + A  ++GL   R++ EPTA A+ 
Sbjct: 130 VLIKMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEPTAAAIA 189

Query: 209 YAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVAVSATGGGVSQIRSLAG-SPIGGEDLL 268
           Y   ++ +      S  EK VLIF++G G  DV++     G+ ++++ AG + +GGED  
Sbjct: 190 YGLDKKAS------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFD 249

Query: 269 QNTMRYLLPNSESIFSHRGINDI----RTMGLLRVATQDAIHELSFQSSVQIDVD-LGNG 328
              + + +      F  +   DI    R +  LR A + A   LS  +   I++D L  G
Sbjct: 250 NRMVNHFVQE----FKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLFEG 309

Query: 329 LKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIEDVSDVVVVGGCSYMPRIRKLL 388
           +     ++R  FE +N  +F KC   V++CL DA+++   V DVV+VGG + +P++++LL
Sbjct: 310 IDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLL 369

Query: 389 MEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNLDLLSIQATPLAIGIRADGNS 448
            +    K L + ++P EA   GAA++ A+ SG G+     DLL +  TPL++G+   G  
Sbjct: 370 QDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQ-DLLLLDVTPLSLGLETAGGV 429

Query: 449 FIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPK 508
              +IP+NTT+P +KE  F+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPAP+
Sbjct: 430 MTVLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPR 489

Query: 509 GVPEITIGMDIDSTNMLRVLAGATLPGAQHPAT 534
           GVP+IT+  DID+  +L V A     G ++  T
Sbjct: 490 GVPQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Sed0020943 vs. ExPASy Swiss-Prot
Match: P09189 (Heat shock cognate 70 kDa protein OS=Petunia hybrida OX=4102 GN=HSP70 PE=2 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 4.5e-74
Identity = 176/513 (34.31%), Postives = 280/513 (54.58%), Query Frame = 0

Query: 29  VGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDETPSGGVSNELSHEYDMLSG 88
           +GID+GT+   + VW   +VE++ N +  +   SYV F D      + +   ++  M   
Sbjct: 10  IGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERL--IGDAAKNQVAMNPI 69

Query: 89  AAIFNMKRLIGRVDTDPVVHAS-KSLPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLAI 148
             +F+ KRLIGR  +DP V +  K  PF V     G +P +        +    EE+ ++
Sbjct: 70  NTVFDAKRLIGRRFSDPSVQSDIKLWPFKV-IPGPGDKPMIVVTYKGEEKQFAAEEISSM 129

Query: 149 FLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQLTRVERACAMAGLQA-RLMPEPTAVALL 208
            L ++K +AE  L   I+N V+T+P  F+  Q    + A  +AGL   R++ EPTA A+ 
Sbjct: 130 VLTKMKEIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAIA 189

Query: 209 YAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVAVSATGGGVSQIRSLAG-SPIGGEDLL 268
           Y   ++ +      S  EK VLIF++G G  DV++     G+ ++++ AG + +GGED  
Sbjct: 190 YGLDKKAS------SAGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFD 249

Query: 269 QNTMRYLLPNSESIFSHRGINDI----RTMGLLRVATQDAIHELSFQSSVQIDVD-LGNG 328
              + + +      F  +   DI    R +  LR A + A   LS  +   I++D L  G
Sbjct: 250 NRMVNHFVQE----FKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG 309

Query: 329 LKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIEDVSDVVVVGGCSYMPRIRKLL 388
           +     ++R  FE +N  +F KC   V++CL DA+++   V DVV+VGG + +P++++LL
Sbjct: 310 IDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLL 369

Query: 389 MEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNLDLLSIQATPLAIGIRADGNS 448
            +    K L + ++P EA   GAA++ A+ SG G+     DLL +  TPL++G+   G  
Sbjct: 370 QDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQ-DLLLLDVTPLSLGLETAGGG 429

Query: 449 FIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPK 508
              +IP+NTT+P +KE  F+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPAP+
Sbjct: 430 MTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPR 489

Query: 509 GVPEITIGMDIDSTNMLRVLAGATLPGAQHPAT 534
           GVP+IT+  DID+  +L V A     G ++  T
Sbjct: 490 GVPQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Sed0020943 vs. ExPASy Swiss-Prot
Match: Q9S9N1 (Heat shock 70 kDa protein 5 OS=Arabidopsis thaliana OX=3702 GN=HSP70-5 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 7.7e-74
Identity = 174/516 (33.72%), Postives = 280/516 (54.26%), Query Frame = 0

Query: 22  STFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDETPSGGVSNELSH 81
           +T  E  +GID+GT+   + VW   +VE++ N +  +   SYV F D      + +   +
Sbjct: 2   ATKSEKAIGIDLGTTYSCVGVWMNDRVEIIPNDQGNRTTPSYVAFTDTERL--IGDAAKN 61

Query: 82  EYDMLSGAAIFNMKRLIGRVDTDPVVHAS-KSLPFLVQTLDIGVRPFVAALVNNVWRSTT 141
           +  +     +F+ KRLIGR  +DP V +     PF V +   G +P +     N  +  +
Sbjct: 62  QVALNPQNTVFDAKRLIGRKFSDPSVQSDILHWPFKVVS-GPGEKPMIVVSYKNEEKQFS 121

Query: 142 PEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQLTRVERACAMAGLQA-RLMPE 201
           PEE+ ++ LV++K +AE  L R ++N V+T+P  F+  Q    + A A++GL   R++ E
Sbjct: 122 PEEISSMVLVKMKEVAEAFLGRTVKNAVVTVPAYFNDSQRQATKDAGAISGLNVLRIINE 181

Query: 202 PTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVAVSATGGGVSQIRSLAG-SP 261
           PTA A+ Y   ++           EK VLIF++G G  DV++     GV ++++ AG + 
Sbjct: 182 PTAAAIAYGLDKKGT------KAGEKNVLIFDLGGGTFDVSLLTIEEGVFEVKATAGDTH 241

Query: 262 IGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVATQDAIHELSFQSSVQIDVD-L 321
           +GGED     + + +             + R +  LR A + A   LS  +   I++D L
Sbjct: 242 LGGEDFDNRLVNHFVAEFRRKHKKDIAGNARALRRLRTACERAKRTLSSTAQTTIEIDSL 301

Query: 322 GNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIEDVSDVVVVGGCSYMPRIR 381
             G+     +SR  FE +N  +F KC + V++ L DA+++   V DVV+VGG + +P+I+
Sbjct: 302 HEGIDFYATISRARFEEMNMDLFRKCMDPVEKVLKDAKLDKSSVHDVVLVGGSTRIPKIQ 361

Query: 382 KLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNLDLLSIQATPLAIGIRAD 441
           +LL +    K L + ++P EA   GAA++ A+ +G G      DLL +   PL++G+   
Sbjct: 362 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILTGEGSEKVQ-DLLLLDVAPLSLGLETA 421

Query: 442 GNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPP 501
           G     +IP+NTT+P +KE  F+T  DNQ   LI VYEGE  R  +N+LLG F++ GIPP
Sbjct: 422 GGVMTVLIPRNTTVPCKKEQVFSTYADNQPGVLIQVYEGERARTRDNNLLGTFELKGIPP 481

Query: 502 APKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPAT 534
           AP+GVP+I +  DID+  +L V A     G ++  T
Sbjct: 482 APRGVPQINVCFDIDANGILNVSAEDKTAGVKNQIT 507

BLAST of Sed0020943 vs. ExPASy Swiss-Prot
Match: P11143 (Heat shock 70 kDa protein OS=Zea mays OX=4577 GN=HSP70 PE=3 SV=2)

HSP 1 Score: 278.9 bits (712), Expect = 1.3e-73
Identity = 175/512 (34.18%), Postives = 279/512 (54.49%), Query Frame = 0

Query: 29  VGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDETPSGGVSNELSHEYDMLSG 88
           +GID+GT+   + VW   +VE++ N +  +   SYV F D      + +   ++  M   
Sbjct: 9   IGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDTERL--IGDAAKNQVAMNPT 68

Query: 89  AAIFNMKRLIGRVDTDPVVHASKSLPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLAIF 148
             +F+ KRLIGR  + P V +S  L +  + L +G +P +        +    EE+ ++ 
Sbjct: 69  NTVFDAKRLIGRRFSSPAVQSSMKL-WPSRHLGLGDKPMIVFNYKGEEKQFAAEEISSMV 128

Query: 149 LVELKMMAEYQLKRPIRNIVLTIPVSFSRFQLTRVERACAMAGLQA-RLMPEPTAVALLY 208
           L+++K +AE  L   I+N V+T+P  F+  Q    + A  +AGL   R++ EPTA A+ Y
Sbjct: 129 LIKMKEIAEAYLGSTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAIAY 188

Query: 209 AQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVAVSATGGGVSQIRSLAG-SPIGGEDLLQ 268
              ++        S  EK VLIF++G G  DV++     G+ ++++ AG + +GGED   
Sbjct: 189 GLDKKAT------SSGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDN 248

Query: 269 NTMRYLLPNSESIFSHRGINDI----RTMGLLRVATQDAIHELSFQSSVQIDVD-LGNGL 328
             + + +      F  +   DI    R +  LR A + A   LS  +   I++D L  G+
Sbjct: 249 RMVNHFVQE----FKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLFEGI 308

Query: 329 KICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIEDVSDVVVVGGCSYMPRIRKLLM 388
                 SR  FE +N  +F KC   V++CL DA+++   V DVV+VGG + +P++++ L 
Sbjct: 309 DFTPRSSRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQ-LQ 368

Query: 389 EITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNLDLLSIQATPLAIGIRADGNSF 448
           +    K L + ++P EA   GAA++ A+ SG G+     DLL +  TPL++G+   G   
Sbjct: 369 DFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNE--RSDLLLLDVTPLSLGLETAGGVM 428

Query: 449 IPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPKG 508
             +IP+NTT+P +KE  F+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPAP+G
Sbjct: 429 TVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRG 488

Query: 509 VPEITIGMDIDSTNMLRVLAGATLPGAQHPAT 534
           VP+IT+  DID  N+L V A     G ++  T
Sbjct: 489 VPQITVTFDIDVNNILNVSAEDKTTGQKNKIT 504

BLAST of Sed0020943 vs. ExPASy TrEMBL
Match: A0A0A0LAC6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G147740 PE=3 SV=1)

HSP 1 Score: 1040.0 bits (2688), Expect = 3.6e-300
Identity = 519/570 (91.05%), Postives = 552/570 (96.84%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKATSTFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60
           MAEPIYTVASDSETTGE++ TSTFPEI VGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDETPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKD+TPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRN+VLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQARLMPEPTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVA 240
           TRVERACAMAGLQ RLMPEPTAVALLYAQQQQQA H++MGSGSEKI LIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSATGGGVSQIRSLAGSPIGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVATQD 300
           VSA GGGVSQIR+LAGSPIGGEDLLQNTM+YLLPNSESIFSHRGI++IR MGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHELSFQSSVQIDVDLGNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIED 360
           AIH+LSFQSSV+I+V LGNG K+CKVLSR++FEMVNSKVFEKC+NLVKQCLHDAR+ IED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARLGIED 360

Query: 361 VSDVVVVGGCSYMPRIRKLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNL 420
           +SDVVVVGGCSY+P+IR LLMEI+KKK LYEG+DPLEAAVSGAALEGA+ASGIGDPFG+L
Sbjct: 361 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKEL FTT+QDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITI MDIDS+NMLRVLAGATLPGAQHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHRKYGTAVDLVTVQKK 571
           PTVDDGHGWCAEALH KYG  ++LVT++KK
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKK 570

BLAST of Sed0020943 vs. ExPASy TrEMBL
Match: A0A5D3D052 (Heat shock 70 kDa protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00370 PE=3 SV=1)

HSP 1 Score: 1038.5 bits (2684), Expect = 1.0e-299
Identity = 520/570 (91.23%), Postives = 551/570 (96.67%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKATSTFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60
           MAEPIYTVASDSETTGE++ TSTFPEI VGIDIGTSQCSIAVWNGS VELLKNTR+QKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 61  RSYVTFKDETPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKD+TPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRN+VLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQARLMPEPTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVA 240
           TRVERACAMAG Q RLMPEPTAVALLYAQQQQQAAH++MGSGSEKI LIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSATGGGVSQIRSLAGSPIGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVATQD 300
           VSA GGGVSQIR+LAGSPIGGEDLLQNTM+YLLPNSESIFSHRGI++IR MGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHELSFQSSVQIDVDLGNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIED 360
           AIH+LSFQSSV+I+V LGNG K+CKVLSR+EFEMVNSKVFEKC+NLVKQCLHDARV IED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 361 VSDVVVVGGCSYMPRIRKLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNL 420
           +SDVVVVGGCSY+P+IRKLLMEI+KKK LYEG+DPLEAAVSGAALEGA+ASGIGDPFG+L
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKEL FTTVQDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITI MDIDS+NMLRVLAGA LPGAQHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHRKYGTAVDLVTVQKK 571
           PTVDDGHGWCAEALH KYG  ++LVT++KK
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKK 570

BLAST of Sed0020943 vs. ExPASy TrEMBL
Match: A0A1S3AXP0 (heat shock 70 kDa protein 8 OS=Cucumis melo OX=3656 GN=LOC103483715 PE=3 SV=1)

HSP 1 Score: 1036.9 bits (2680), Expect = 3.0e-299
Identity = 519/570 (91.05%), Postives = 550/570 (96.49%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKATSTFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60
           MAEPIYTVASDSETTGE++ TSTFPEI VGIDIGTSQCSIAVWNGS VELLKNTR+QKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 61  RSYVTFKDETPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKD+TPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRN+VLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQARLMPEPTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVA 240
           TRVERACAMAG Q RLMPEPTAVALLYAQQQQQAAH++MGSGSEKI LIFNMGAGYCD A
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240

Query: 241 VSATGGGVSQIRSLAGSPIGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVATQD 300
           VSA GGGVSQIR+LAGSPIGGEDLLQNTM+YLLPNSESIFSHRGI++IR MGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHELSFQSSVQIDVDLGNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIED 360
           AIH+LSFQSSV+I+V LGNG K+CKVLSR+EFEMVNSKVFEKC+NLVKQCLHDARV IED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 361 VSDVVVVGGCSYMPRIRKLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNL 420
           +SDVVVVGGCSY+P+IRKLLMEI+KKK LYEG+DPLEAAVSGAALEGA+ASGIGDPFG+L
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKEL FTTVQDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITI MDIDS+NMLRVLAGA LPGAQHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHRKYGTAVDLVTVQKK 571
           PTVDDGHGWCAEALH KYG  ++LVT++KK
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKK 570

BLAST of Sed0020943 vs. ExPASy TrEMBL
Match: A0A6J1C992 (heat shock 70 kDa protein 8 OS=Momordica charantia OX=3673 GN=LOC111009482 PE=3 SV=1)

HSP 1 Score: 1023.8 bits (2646), Expect = 2.7e-295
Identity = 514/570 (90.18%), Postives = 545/570 (95.61%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKATSTFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60
           MAEPIYTVASDSETTGEEK TSTFPEI VGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM
Sbjct: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDETPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKD+TPSGGVS+EL HEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSSELCHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQL 180
           DIGVRPFVAALVNN+WRSTTPEEVLAIFLVELKMMAEYQLKRPIRN+VLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNMWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQARLMPEPTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVA 240
           TRVERACAMAGLQ RLMPEPTAVALLYAQQQ QA HENMGSGSEKI L+FNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQHQAVHENMGSGSEKIALVFNMGAGYCDVA 240

Query: 241 VSATGGGVSQIRSLAGSPIGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVATQD 300
           +SAT GGVSQIR+L GSPIGGEDLLQNTM+YLLPNSESIFS+ GI++IR MGLLRVATQD
Sbjct: 241 ISATAGGVSQIRALTGSPIGGEDLLQNTMKYLLPNSESIFSNHGIDEIRRMGLLRVATQD 300

Query: 301 AIHELSFQSSVQIDVDLGNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIED 360
           AI +LSFQ+S QIDV+LGNG KICKVLSR+EFEMVNSKVFEKC+NLVKQCLHDARVEIED
Sbjct: 301 AILKLSFQTSAQIDVNLGNGSKICKVLSREEFEMVNSKVFEKCENLVKQCLHDARVEIED 360

Query: 361 VSDVVVVGGCSYMPRIRKLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNL 420
           +SDVVVVGGCS +P+IRKLLMEI+KKK LYE +DPLEAAVSGAA+EGAVASGIGDPFG+L
Sbjct: 361 LSDVVVVGGCSSIPKIRKLLMEISKKKALYEEIDPLEAAVSGAAIEGAVASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQ TPLAIGIRADGN+FIPIIPKNTTMPARKEL FTTVQDNQSEALIVVYEGEE R
Sbjct: 421 DLLSIQVTPLAIGIRADGNNFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEEAR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITI MDIDS+N LRVLAGATLPGAQHPATPFMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNTLRVLAGATLPGAQHPATPFMEVKM 540

Query: 541 PTVDDGHGWCAEALHRKYGTAVDLVTVQKK 571
           PT+DDGH WCAEALH K+G ++DLVT++KK
Sbjct: 541 PTIDDGHSWCAEALHGKHGVSMDLVTLRKK 570

BLAST of Sed0020943 vs. ExPASy TrEMBL
Match: A0A6J1IWD5 (heat shock 70 kDa protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111479090 PE=3 SV=1)

HSP 1 Score: 1019.2 bits (2634), Expect = 6.6e-294
Identity = 515/571 (90.19%), Postives = 545/571 (95.45%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKATSTFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60
           MAEPIYTVASDSETTGE+++   FPEI VGIDIGTSQCSIA WNGSQVELLKNTRNQKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRSAPIFPEIAVGIDIGTSQCSIAFWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDETPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKD+TPSGGV NELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVINELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAE+QLKRPIRN+VLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEHQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQARLMPEPTAVALLYA-QQQQQAAHENMGSGSEKIVLIFNMGAGYCDV 240
           TRVERACAMAGLQ RLMPEPTAVALLYA QQQQQA HENMGSGSEKI LIFNMGAGYCDV
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQQAVHENMGSGSEKIALIFNMGAGYCDV 240

Query: 241 AVSATGGGVSQIRSLAGSPIGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVATQ 300
           AVSATGGGVSQI++LAGSPIGGEDLLQNTM+YLLPN ESIFSHRGI++IR MGLLRVATQ
Sbjct: 241 AVSATGGGVSQIKALAGSPIGGEDLLQNTMKYLLPNVESIFSHRGIDEIRRMGLLRVATQ 300

Query: 301 DAIHELSFQSSVQIDVDLGNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIE 360
           DAIH+LSFQSSVQIDV+LGNG K+CKVLSR+EFEMVNS+VFEKCKNLV QCLHDARV I+
Sbjct: 301 DAIHKLSFQSSVQIDVNLGNGSKLCKVLSREEFEMVNSEVFEKCKNLVNQCLHDARVGID 360

Query: 361 DVSDVVVVGGCSYMPRIRKLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGN 420
           D+SDVVVVGGCS +P+IR LLM+ +KKK LYEGM+PLEAAVSGAALEGAVASGI DPFG+
Sbjct: 361 DLSDVVVVGGCSNIPKIRSLLMQKSKKKALYEGMNPLEAAVSGAALEGAVASGIADPFGS 420

Query: 421 LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEET 480
           LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKEL FTTV+DNQSEALIVVYEGEET
Sbjct: 421 LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVKDNQSEALIVVYEGEET 480

Query: 481 RAEENHLLGYFKIIGIPPAPKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPATPFMEVK 540
           RAEENHLLGYFKIIGIPPAPKG+PEITI MDIDS+NMLRVLAGA LPGA HPATPFMEVK
Sbjct: 481 RAEENHLLGYFKIIGIPPAPKGLPEITICMDIDSSNMLRVLAGARLPGAHHPATPFMEVK 540

Query: 541 MPTVDDGHGWCAEALHRKYGTAVDLVTVQKK 571
           MPTVDDGHGWCAEALH KYG A+DLV++QKK
Sbjct: 541 MPTVDDGHGWCAEALHGKYGAAMDLVSLQKK 571

BLAST of Sed0020943 vs. TAIR 10
Match: AT2G32120.1 (heat-shock protein 70T-2 )

HSP 1 Score: 831.6 bits (2147), Expect = 3.7e-241
Identity = 406/574 (70.73%), Postives = 488/574 (85.02%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKATS--TFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQK 60
           MAE  YTVASDSE TGEEK++S  + PEI +GIDIGTSQCSIAVWNGSQV +L+NTRNQK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 61  IMRSYVTFKDETPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQ 120
           +++S+VTFKDE P+GGVSN+L+HE +ML+GAAIFNMKRL+GRVDTDPVVHASK+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 121 TLDIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRF 180
           TLDIGVRPF+AALVNN WRSTTPEEVLAIFLVEL++MAE QLKRP+RN+VLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 181 QLTRVERACAMAGLQA-RLMPEPTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYC 240
           QLTR ERACAMAGL   RLMPEPTA+ALLYAQQQQ   H+NMGSGSE++ +IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 241 DVAVSATGGGVSQIRSLAGSPIGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVA 300
           DVAV+AT GGVSQI++LAGSPIGGED+LQNT+R++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 301 TQDAIHELSFQSSVQIDVDLGNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVE 360
            QDAIH L+ Q +VQI+VDLGNG KI KVL R EFE VN KVFE+C+ LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 361 IEDVSDVVVVGGCSYMPRIRKLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPF 420
             D+ D+++VGGCSY+P++R ++  + KK  +Y+G++PLEAAV GAALEGAV SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 421 GNLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGE 480
           G+LDLL+IQATPLA+G+RA+GN FIP+IP+NT +PARK+L FTTVQDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 481 ETRAEENHLLGYFKIIGIPPAPKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPATPFME 540
               EENHLLGYFK++GIPPAPKGVPEI + MDID++N LRV A   +PG+  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 541 VKMPTVDDGHGWCAEALHRKYGTAVDLVTVQKKI 572
           V+MPTVDDGHGWCA+AL+ KYG  +DL+T+Q+K+
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRKM 563

BLAST of Sed0020943 vs. TAIR 10
Match: AT2G32120.2 (heat-shock protein 70T-2 )

HSP 1 Score: 831.6 bits (2147), Expect = 3.7e-241
Identity = 406/574 (70.73%), Postives = 488/574 (85.02%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKATS--TFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQK 60
           MAE  YTVASDSE TGEEK++S  + PEI +GIDIGTSQCSIAVWNGSQV +L+NTRNQK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 61  IMRSYVTFKDETPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQ 120
           +++S+VTFKDE P+GGVSN+L+HE +ML+GAAIFNMKRL+GRVDTDPVVHASK+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 121 TLDIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRF 180
           TLDIGVRPF+AALVNN WRSTTPEEVLAIFLVEL++MAE QLKRP+RN+VLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 181 QLTRVERACAMAGLQA-RLMPEPTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYC 240
           QLTR ERACAMAGL   RLMPEPTA+ALLYAQQQQ   H+NMGSGSE++ +IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 241 DVAVSATGGGVSQIRSLAGSPIGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVA 300
           DVAV+AT GGVSQI++LAGSPIGGED+LQNT+R++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 301 TQDAIHELSFQSSVQIDVDLGNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVE 360
            QDAIH L+ Q +VQI+VDLGNG KI KVL R EFE VN KVFE+C+ LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 361 IEDVSDVVVVGGCSYMPRIRKLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPF 420
             D+ D+++VGGCSY+P++R ++  + KK  +Y+G++PLEAAV GAALEGAV SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 421 GNLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGE 480
           G+LDLL+IQATPLA+G+RA+GN FIP+IP+NT +PARK+L FTTVQDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 481 ETRAEENHLLGYFKIIGIPPAPKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPATPFME 540
               EENHLLGYFK++GIPPAPKGVPEI + MDID++N LRV A   +PG+  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 541 VKMPTVDDGHGWCAEALHRKYGTAVDLVTVQKKI 572
           V+MPTVDDGHGWCA+AL+ KYG  +DL+T+Q+K+
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRKM 563

BLAST of Sed0020943 vs. TAIR 10
Match: AT3G12580.1 (heat shock protein 70 )

HSP 1 Score: 284.6 bits (727), Expect = 1.7e-76
Identity = 176/513 (34.31%), Postives = 285/513 (55.56%), Query Frame = 0

Query: 29  VGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDETPSGGVSNELSHEYDMLSG 88
           +GID+GT+   + VW   +VE++ N +  +   SYV F D      + +   ++  M   
Sbjct: 10  IGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNPT 69

Query: 89  AAIFNMKRLIGRVDTDPVVHASKS-LPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLAI 148
             +F+ KRLIGR  +DP V A KS  PF V +   G +P +        +  + EE+ ++
Sbjct: 70  NTVFDAKRLIGRRYSDPSVQADKSHWPFKVVS-GPGEKPMIVVNHKGEEKQFSAEEISSM 129

Query: 149 FLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQLTRVERACAMAGLQA-RLMPEPTAVALL 208
            L++++ +AE  L  P++N V+T+P  F+  Q    + A  ++GL   R++ EPTA A+ 
Sbjct: 130 VLIKMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEPTAAAIA 189

Query: 209 YAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVAVSATGGGVSQIRSLAG-SPIGGEDLL 268
           Y   ++ +      S  EK VLIF++G G  DV++     G+ ++++ AG + +GGED  
Sbjct: 190 YGLDKKAS------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFD 249

Query: 269 QNTMRYLLPNSESIFSHRGINDI----RTMGLLRVATQDAIHELSFQSSVQIDVD-LGNG 328
              + + +      F  +   DI    R +  LR A + A   LS  +   I++D L  G
Sbjct: 250 NRMVNHFVQE----FKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLFEG 309

Query: 329 LKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIEDVSDVVVVGGCSYMPRIRKLL 388
           +     ++R  FE +N  +F KC   V++CL DA+++   V DVV+VGG + +P++++LL
Sbjct: 310 IDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLL 369

Query: 389 MEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNLDLLSIQATPLAIGIRADGNS 448
            +    K L + ++P EA   GAA++ A+ SG G+     DLL +  TPL++G+   G  
Sbjct: 370 QDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQ-DLLLLDVTPLSLGLETAGGV 429

Query: 449 FIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPK 508
              +IP+NTT+P +KE  F+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPAP+
Sbjct: 430 MTVLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPR 489

Query: 509 GVPEITIGMDIDSTNMLRVLAGATLPGAQHPAT 534
           GVP+IT+  DID+  +L V A     G ++  T
Sbjct: 490 GVPQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Sed0020943 vs. TAIR 10
Match: AT1G16030.1 (heat shock protein 70B )

HSP 1 Score: 279.6 bits (714), Expect = 5.5e-75
Identity = 174/516 (33.72%), Postives = 280/516 (54.26%), Query Frame = 0

Query: 22  STFPEIVVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDETPSGGVSNELSH 81
           +T  E  +GID+GT+   + VW   +VE++ N +  +   SYV F D      + +   +
Sbjct: 2   ATKSEKAIGIDLGTTYSCVGVWMNDRVEIIPNDQGNRTTPSYVAFTDTERL--IGDAAKN 61

Query: 82  EYDMLSGAAIFNMKRLIGRVDTDPVVHAS-KSLPFLVQTLDIGVRPFVAALVNNVWRSTT 141
           +  +     +F+ KRLIGR  +DP V +     PF V +   G +P +     N  +  +
Sbjct: 62  QVALNPQNTVFDAKRLIGRKFSDPSVQSDILHWPFKVVS-GPGEKPMIVVSYKNEEKQFS 121

Query: 142 PEEVLAIFLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQLTRVERACAMAGLQA-RLMPE 201
           PEE+ ++ LV++K +AE  L R ++N V+T+P  F+  Q    + A A++GL   R++ E
Sbjct: 122 PEEISSMVLVKMKEVAEAFLGRTVKNAVVTVPAYFNDSQRQATKDAGAISGLNVLRIINE 181

Query: 202 PTAVALLYAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVAVSATGGGVSQIRSLAG-SP 261
           PTA A+ Y   ++           EK VLIF++G G  DV++     GV ++++ AG + 
Sbjct: 182 PTAAAIAYGLDKKGT------KAGEKNVLIFDLGGGTFDVSLLTIEEGVFEVKATAGDTH 241

Query: 262 IGGEDLLQNTMRYLLPNSESIFSHRGINDIRTMGLLRVATQDAIHELSFQSSVQIDVD-L 321
           +GGED     + + +             + R +  LR A + A   LS  +   I++D L
Sbjct: 242 LGGEDFDNRLVNHFVAEFRRKHKKDIAGNARALRRLRTACERAKRTLSSTAQTTIEIDSL 301

Query: 322 GNGLKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIEDVSDVVVVGGCSYMPRIR 381
             G+     +SR  FE +N  +F KC + V++ L DA+++   V DVV+VGG + +P+I+
Sbjct: 302 HEGIDFYATISRARFEEMNMDLFRKCMDPVEKVLKDAKLDKSSVHDVVLVGGSTRIPKIQ 361

Query: 382 KLLMEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNLDLLSIQATPLAIGIRAD 441
           +LL +    K L + ++P EA   GAA++ A+ +G G      DLL +   PL++G+   
Sbjct: 362 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILTGEGSEKVQ-DLLLLDVAPLSLGLETA 421

Query: 442 GNSFIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPP 501
           G     +IP+NTT+P +KE  F+T  DNQ   LI VYEGE  R  +N+LLG F++ GIPP
Sbjct: 422 GGVMTVLIPRNTTVPCKKEQVFSTYADNQPGVLIQVYEGERARTRDNNLLGTFELKGIPP 481

Query: 502 APKGVPEITIGMDIDSTNMLRVLAGATLPGAQHPAT 534
           AP+GVP+I +  DID+  +L V A     G ++  T
Sbjct: 482 APRGVPQINVCFDIDANGILNVSAEDKTAGVKNQIT 507

BLAST of Sed0020943 vs. TAIR 10
Match: AT5G02500.1 (heat shock cognate protein 70-1 )

HSP 1 Score: 277.3 bits (708), Expect = 2.7e-74
Identity = 173/513 (33.72%), Postives = 280/513 (54.58%), Query Frame = 0

Query: 29  VGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDETPSGGVSNELSHEYDMLSG 88
           +GID+GT+   + VW   +VE++ N +  +   SYV F D      + +   ++  M   
Sbjct: 10  IGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNPV 69

Query: 89  AAIFNMKRLIGRVDTDPVVHASKSL-PFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLAI 148
             +F+ KRLIGR  +D  V +   L PF +Q      +P +        +    EE+ ++
Sbjct: 70  NTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQA-GPADKPMIYVEYKGEEKEFAAEEISSM 129

Query: 149 FLVELKMMAEYQLKRPIRNIVLTIPVSFSRFQLTRVERACAMAGLQA-RLMPEPTAVALL 208
            L++++ +AE  L   I+N V+T+P  F+  Q    + A  +AGL   R++ EPTA A+ 
Sbjct: 130 VLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAIA 189

Query: 209 YAQQQQQAAHENMGSGSEKIVLIFNMGAGYCDVAVSATGGGVSQIRSLAG-SPIGGEDLL 268
           Y   ++        S  EK VLIF++G G  DV++     G+ ++++ AG + +GGED  
Sbjct: 190 YGLDKKAT------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFD 249

Query: 269 QNTMRYLLPNSESIFSHRGINDI----RTMGLLRVATQDAIHELSFQSSVQIDVD-LGNG 328
              + + +      F  +   DI    R +  LR + + A   LS  +   I++D L  G
Sbjct: 250 NRMVNHFVQE----FKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEIDSLYEG 309

Query: 329 LKICKVLSRDEFEMVNSKVFEKCKNLVKQCLHDARVEIEDVSDVVVVGGCSYMPRIRKLL 388
           +     ++R  FE +N  +F KC   V++CL DA+++   V DVV+VGG + +P++++LL
Sbjct: 310 IDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLL 369

Query: 389 MEITKKKVLYEGMDPLEAAVSGAALEGAVASGIGDPFGNLDLLSIQATPLAIGIRADGNS 448
            +    K L + ++P EA   GAA++GA+ SG G+     DLL +  TPL++G+   G  
Sbjct: 370 QDFFNGKELCKSINPDEAVAYGAAVQGAILSGEGNEKVQ-DLLLLDVTPLSLGLETAGGV 429

Query: 449 FIPIIPKNTTMPARKELAFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPK 508
              +IP+NTT+P +KE  F+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPAP+
Sbjct: 430 MTTLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPR 489

Query: 509 GVPEITIGMDIDSTNMLRVLAGATLPGAQHPAT 534
           GVP+IT+  DID+  +L V A     G ++  T
Sbjct: 490 GVPQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004134140.17.4e-30091.05heat shock 70 kDa protein 8 [Cucumis sativus] >XP_011650982.1 heat shock 70 kDa ... [more]
KAA0049367.12.2e-29991.23heat shock 70 kDa protein 8 [Cucumis melo var. makuwa] >TYK17192.1 heat shock 70... [more]
XP_008438682.16.3e-29991.05PREDICTED: heat shock 70 kDa protein 8 [Cucumis melo][more]
XP_038897373.11.1e-29891.05heat shock 70 kDa protein 8 [Benincasa hispida][more]
XP_022138266.15.5e-29590.18heat shock 70 kDa protein 8 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9SKY85.2e-24070.73Heat shock 70 kDa protein 8 OS=Arabidopsis thaliana OX=3702 GN=HSP70-8 PE=1 SV=1[more]
Q9LHA82.4e-7534.31Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1[more]
P091894.5e-7434.31Heat shock cognate 70 kDa protein OS=Petunia hybrida OX=4102 GN=HSP70 PE=2 SV=1[more]
Q9S9N17.7e-7433.72Heat shock 70 kDa protein 5 OS=Arabidopsis thaliana OX=3702 GN=HSP70-5 PE=2 SV=1[more]
P111431.3e-7334.18Heat shock 70 kDa protein OS=Zea mays OX=4577 GN=HSP70 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LAC63.6e-30091.05Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G147740 PE=3 SV=1[more]
A0A5D3D0521.0e-29991.23Heat shock 70 kDa protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3AXP03.0e-29991.05heat shock 70 kDa protein 8 OS=Cucumis melo OX=3656 GN=LOC103483715 PE=3 SV=1[more]
A0A6J1C9922.7e-29590.18heat shock 70 kDa protein 8 OS=Momordica charantia OX=3673 GN=LOC111009482 PE=3 ... [more]
A0A6J1IWD56.6e-29490.19heat shock 70 kDa protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111479090 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT2G32120.13.7e-24170.73heat-shock protein 70T-2 [more]
AT2G32120.23.7e-24170.73heat-shock protein 70T-2 [more]
AT3G12580.11.7e-7634.31heat shock protein 70 [more]
AT1G16030.15.5e-7533.72heat shock protein 70B [more]
AT5G02500.12.7e-7433.72heat shock cognate protein 70-1 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00301HEATSHOCK70coord: 393..413
score: 39.8
coord: 502..518
score: 35.07
coord: 421..440
score: 36.79
coord: 167..187
score: 28.42
coord: 27..40
score: 40.08
coord: 55..67
score: 27.83
coord: 361..377
score: 38.12
NoneNo IPR availableGENE3D3.90.640.10Actin; Chain A, domain 4coord: 261..342
e-value: 2.9E-80
score: 271.7
NoneNo IPR availableGENE3D3.30.30.30coord: 92..147
e-value: 2.9E-80
score: 271.7
NoneNo IPR availableGENE3D3.30.420.40coord: 29..408
e-value: 2.9E-80
score: 271.7
NoneNo IPR availableGENE3D3.30.420.40coord: 212..390
e-value: 2.9E-80
score: 271.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR19375:SF101HEAT SHOCK 70 KDA PROTEIN 8coord: 4..570
NoneNo IPR availableCDDcd10170HSP70_NBDcoord: 29..408
e-value: 8.4033E-70
score: 227.496
IPR029047Heat shock protein 70kD, peptide-binding domain superfamilyGENE3D2.60.34.10Substrate Binding Domain Of DNAk; Chain A, domain 1coord: 419..531
e-value: 8.2E-33
score: 115.5
IPR029047Heat shock protein 70kD, peptide-binding domain superfamilySUPERFAMILY100920Heat shock protein 70kD (HSP70), peptide-binding domaincoord: 423..522
IPR013126Heat shock protein 70 familyPFAMPF00012HSP70coord: 28..525
e-value: 9.1E-90
score: 301.6
IPR013126Heat shock protein 70 familyPANTHERPTHR19375HEAT SHOCK PROTEIN 70KDAcoord: 4..570
IPR018181Heat shock protein 70, conserved sitePROSITEPS01036HSP70_3coord: 364..378
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 224..409
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 27..209

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0020943.1Sed0020943.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034620 cellular response to unfolded protein
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0042026 protein refolding
biological_process GO:0009408 response to heat
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0031072 heat shock protein binding
molecular_function GO:0051787 misfolded protein binding
molecular_function GO:0044183 protein folding chaperone
molecular_function GO:0051082 unfolded protein binding