Sed0020777 (gene) Chayote v1

Overview


Name	Sed0020777
Type	gene
Organism	Sechium edule (Chayote v1)
Description	indole-3-acetic acid-amido synthetase GH3.6-like
Location	LG01: 5104243 .. 5106390 (+)
RNA-Seq Expression	Sed0020777
Synteny	Sed0020777

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTATGTGGGTTGCAGCTTTATTTTTCAAAACTTCAAATCCTCAAAAAATGTCTCATGAGGCAGAAAACCTGCTAGAGGAGAAAGACAAGCAAGCGTTGCAACATATTGAAGATATAACGAGCAACGCCGGCGAAATCCAACGACAAACTCTGGCCGAAATTCTGGCAACAAATGCTGATGTTGAGTACTTGCAGCAACATGGTCTTAATGGATCTACTGATGCTTCAACTTTCAAGAACTTTATACCGGTCATATCTTACGAACAATTAAAACCTTACATAGATCGTATTGCAAATGGTGACAATTCTCCAATCCTATGCACAGAACCCATTACAGAGTTCTTTATCAGGTGACCCTTTTCCCTAAAACACAATTTTTAAATCTTCTTCTTTTTTTAGATGTGGGATTTAGTTAATTCTAAAGATATTGTATTTCTGTTGTAGCACTGGAACAACCGGGGGAGAACAAAAGTTAATTCCAATATTTGAAAAAGAGTATGTGAGAAGATTGTCTTTTTTTAATTATGTGATCCCAAGAATGAAACAGTTGTTTCCAAATTCCCACAAAGGAAAAGAAATGTTCTTCTGTTTCACCAAGGCTGAGTCTACAACTAAAGCTGGAATTATTGCTTATTCTACATCAACAAGTTTGTGCAAGAGATCACACTTCAACAATATACAAAGTGGTGGTAACACGAGTCCGATCGACGTCACTTTATGCACAGACACATACCAGAGCACATATTCTCAGTTGCTTTGTGGGCTCTATCAAAATGAGAAGGTTCATAAATTGGGTGCTCTTTTTGTTTCAACTTTAATTTATGTTCTTAAGTTCCTCGAAAACCATTGGATTCTCTTGGCTAATGATATTCGAACCGGAACCATAGATCCAAAAATTATAGACTCATCCGTGAGAGAATCGGTTATGAACATTCTTAAACCGAATCCACAACTTGCAGATTTCATTGAAATTGAATGTAACAAAGGGTCATGGGAAGGGATCATTCCAAGATTGTGGCCAAACGCCAATTACATTAAAGCTATTGCTTCAGGAAGCATGTCACAATACGTTCCCCTGATTAATTACTATAGTAATAACCTCCCTCTTATTTCAGATCACTATGGTTCTTCAGAATGCTTCCTTGGCTTGAACTTAGATCCATTATGCAAACCTGAAGAAATCAGCTACACTTTAGTCCCCGACATGGCATATTTCGAGTTCTTGCCAATTGAAAGAGAAAAGGCTAATGGAGAAGCAAGGCAGGAATTAGTTGATCTTGTGGATGTGAAGTTAGGACAAGAATATGAGCTGGTCATCACCACTTATGGTGGCCTCTACCGCTACTGCATGGGTGACATCCTTCGCGTGAAAGGGTTTAAAAACAAAGCCCCAAACTTTAGTTTTGTAGGTAGGAAAAACATAATTCTAACTGTGGATTATGAGAAAACAGAGGAGAGTGAGCTTCATAACGCTGTACAAGAAGCTGCTCGTCTTATAAGCCCATTTGGAGCTACTATTGTTGACTACACTAGCTATGCAGATACAACAACAATTCCTGGACATTATGTGTTGTATTGGGAAATTCATGTTAAGGAAGAAAGCTCTATTATTTCATCTTCCATCTATGAGGATTGTTGCCTGATTGTTGAAGAGTTGTTGAATCCTGTGTATCGTTACGGAAGATCTCAACAAAAATGTATAAATCCACTAGAGATCAAAATTGTAGAGAGTGGAACTTTTGAAAAACTAGTGGAGCGAGCAGTGAATCAAGGGGCATCAATTAGTCAATACAAGACACCTCGAAGCATAAAATCGACGCAGATCATCGAGCTCTTGGATGCAAGAGTTTTGTCTAACCATTTTAGCCAAGAATGCCCGAAATGGGATCCCAGTGGTCGACACCCTCTCCTTTCCTCCTAGTTTACTGTGACCAAACAATAATAAATAATAAGAGAAAATTAAACATCACATTTCAATTATTATCGGTTTAAGTGTTTGTTTATGTCTTTTTGGGTGATTTTGCTTACTCTCTGTTGTTTTATGGAAAGCTTGTTCATAAGCACTGTTTATGTCTTTTTGTAGTCCCCTTGTTCTGATTGCTCTCAGCTACGAAATAAAAGAATAATATTTTCGAGTTCTGAATA

mRNA sequence

ATGTTTATGTGGGTTGCAGCTTTATTTTTCAAAACTTCAAATCCTCAAAAAATGTCTCATGAGGCAGAAAACCTGCTAGAGGAGAAAGACAAGCAAGCGTTGCAACATATTGAAGATATAACGAGCAACGCCGGCGAAATCCAACGACAAACTCTGGCCGAAATTCTGGCAACAAATGCTGATGTTGAGTACTTGCAGCAACATGGTCTTAATGGATCTACTGATGCTTCAACTTTCAAGAACTTTATACCGGTCATATCTTACGAACAATTAAAACCTTACATAGATCGTATTGCAAATGGTGACAATTCTCCAATCCTATGCACAGAACCCATTACAGAGTTCTTTATCAGCACTGGAACAACCGGGGGAGAACAAAAGTTAATTCCAATATTTGAAAAAGAGTATGTGAGAAGATTGTCTTTTTTTAATTATGTGATCCCAAGAATGAAACAGTTGTTTCCAAATTCCCACAAAGGAAAAGAAATGTTCTTCTGTTTCACCAAGGCTGAGTCTACAACTAAAGCTGGAATTATTGCTTATTCTACATCAACAAGTTTGTGCAAGAGATCACACTTCAACAATATACAAAGTGGTGGTAACACGAGTCCGATCGACGTCACTTTATGCACAGACACATACCAGAGCACATATTCTCAGTTGCTTTGTGGGCTCTATCAAAATGAGAAGGTTCATAAATTGGGTGCTCTTTTTGTTTCAACTTTAATTTATGTTCTTAAGTTCCTCGAAAACCATTGGATTCTCTTGGCTAATGATATTCGAACCGGAACCATAGATCCAAAAATTATAGACTCATCCGTGAGAGAATCGGTTATGAACATTCTTAAACCGAATCCACAACTTGCAGATTTCATTGAAATTGAATGTAACAAAGGGTCATGGGAAGGGATCATTCCAAGATTGTGGCCAAACGCCAATTACATTAAAGCTATTGCTTCAGGAAGCATGTCACAATACGTTCCCCTGATTAATTACTATAGTAATAACCTCCCTCTTATTTCAGATCACTATGGTTCTTCAGAATGCTTCCTTGGCTTGAACTTAGATCCATTATGCAAACCTGAAGAAATCAGCTACACTTTAGTCCCCGACATGGCATATTTCGAGTTCTTGCCAATTGAAAGAGAAAAGGCTAATGGAGAAGCAAGGCAGGAATTAGTTGATCTTGTGGATGTGAAGTTAGGACAAGAATATGAGCTGGTCATCACCACTTATGGTGGCCTCTACCGCTACTGCATGGGTGACATCCTTCGCGTGAAAGGGTTTAAAAACAAAGCCCCAAACTTTAGTTTTGTAGGTAGGAAAAACATAATTCTAACTGTGGATTATGAGAAAACAGAGGAGAGTGAGCTTCATAACGCTGTACAAGAAGCTGCTCGTCTTATAAGCCCATTTGGAGCTACTATTGTTGACTACACTAGCTATGCAGATACAACAACAATTCCTGGACATTATGTGTTGTATTGGGAAATTCATGTTAAGGAAGAAAGCTCTATTATTTCATCTTCCATCTATGAGGATTGTTGCCTGATTGTTGAAGAGTTGTTGAATCCTGTGTATCGTTACGGAAGATCTCAACAAAAATGTATAAATCCACTAGAGATCAAAATTGTAGAGAGTGGAACTTTTGAAAAACTAGTGGAGCGAGCAGTGAATCAAGGGGCATCAATTAGTCAATACAAGACACCTCGAAGCATAAAATCGACGCAGATCATCGAGCTCTTGGATGCAAGAGTTTTGTCTAACCATTTTAGCCAAGAATGCCCGAAATGGGATCCCAGTGGTCGACACCCTCTCCTTTCCTCCTAGTTTACTGTGACCAAACAATAATAAATAATAAGAGAAAATTAAACATCACATTTCAATTATTATCGGTTTAAGTGTTTGTTTATGTCTTTTTGGGTGATTTTGCTTACTCTCTGTTGTTTTATGGAAAGCTTGTTCATAAGCACTGTTTATGTCTTTTTGTAGTCCCCTTGTTCTGATTGCTCTCAGCTACGAAATAAAAGAATAATATTTTCGAGTTCTGAATA

Coding sequence (CDS)

ATGTTTATGTGGGTTGCAGCTTTATTTTTCAAAACTTCAAATCCTCAAAAAATGTCTCATGAGGCAGAAAACCTGCTAGAGGAGAAAGACAAGCAAGCGTTGCAACATATTGAAGATATAACGAGCAACGCCGGCGAAATCCAACGACAAACTCTGGCCGAAATTCTGGCAACAAATGCTGATGTTGAGTACTTGCAGCAACATGGTCTTAATGGATCTACTGATGCTTCAACTTTCAAGAACTTTATACCGGTCATATCTTACGAACAATTAAAACCTTACATAGATCGTATTGCAAATGGTGACAATTCTCCAATCCTATGCACAGAACCCATTACAGAGTTCTTTATCAGCACTGGAACAACCGGGGGAGAACAAAAGTTAATTCCAATATTTGAAAAAGAGTATGTGAGAAGATTGTCTTTTTTTAATTATGTGATCCCAAGAATGAAACAGTTGTTTCCAAATTCCCACAAAGGAAAAGAAATGTTCTTCTGTTTCACCAAGGCTGAGTCTACAACTAAAGCTGGAATTATTGCTTATTCTACATCAACAAGTTTGTGCAAGAGATCACACTTCAACAATATACAAAGTGGTGGTAACACGAGTCCGATCGACGTCACTTTATGCACAGACACATACCAGAGCACATATTCTCAGTTGCTTTGTGGGCTCTATCAAAATGAGAAGGTTCATAAATTGGGTGCTCTTTTTGTTTCAACTTTAATTTATGTTCTTAAGTTCCTCGAAAACCATTGGATTCTCTTGGCTAATGATATTCGAACCGGAACCATAGATCCAAAAATTATAGACTCATCCGTGAGAGAATCGGTTATGAACATTCTTAAACCGAATCCACAACTTGCAGATTTCATTGAAATTGAATGTAACAAAGGGTCATGGGAAGGGATCATTCCAAGATTGTGGCCAAACGCCAATTACATTAAAGCTATTGCTTCAGGAAGCATGTCACAATACGTTCCCCTGATTAATTACTATAGTAATAACCTCCCTCTTATTTCAGATCACTATGGTTCTTCAGAATGCTTCCTTGGCTTGAACTTAGATCCATTATGCAAACCTGAAGAAATCAGCTACACTTTAGTCCCCGACATGGCATATTTCGAGTTCTTGCCAATTGAAAGAGAAAAGGCTAATGGAGAAGCAAGGCAGGAATTAGTTGATCTTGTGGATGTGAAGTTAGGACAAGAATATGAGCTGGTCATCACCACTTATGGTGGCCTCTACCGCTACTGCATGGGTGACATCCTTCGCGTGAAAGGGTTTAAAAACAAAGCCCCAAACTTTAGTTTTGTAGGTAGGAAAAACATAATTCTAACTGTGGATTATGAGAAAACAGAGGAGAGTGAGCTTCATAACGCTGTACAAGAAGCTGCTCGTCTTATAAGCCCATTTGGAGCTACTATTGTTGACTACACTAGCTATGCAGATACAACAACAATTCCTGGACATTATGTGTTGTATTGGGAAATTCATGTTAAGGAAGAAAGCTCTATTATTTCATCTTCCATCTATGAGGATTGTTGCCTGATTGTTGAAGAGTTGTTGAATCCTGTGTATCGTTACGGAAGATCTCAACAAAAATGTATAAATCCACTAGAGATCAAAATTGTAGAGAGTGGAACTTTTGAAAAACTAGTGGAGCGAGCAGTGAATCAAGGGGCATCAATTAGTCAATACAAGACACCTCGAAGCATAAAATCGACGCAGATCATCGAGCTCTTGGATGCAAGAGTTTTGTCTAACCATTTTAGCCAAGAATGCCCGAAATGGGATCCCAGTGGTCGACACCCTCTCCTTTCCTCCTAG

Protein sequence

MFMWVAALFFKTSNPQKMSHEAENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYVRRLSFFNYVIPRMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSGGNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREKANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVGRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGHYVLYWEIHVKEESSIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGTFEKLVERAVNQGASISQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWDPSGRHPLLSS

Homology

[Close]

BLAST of Sed0020777 vs. NCBI nr
Match: KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 864.0 bits (2231), Expect = 7.8e-247
Identity = 418/593 (70.49%), Postives = 501/593 (84.49%), Query Frame = 0


Query: 18  MSHEAENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDAS 77
           M+H  E   +E+DKQALQHIED+T +A +IQRQ L +IL+TNADVEYLQQHGL+GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 78  TFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYV 137
           TFKN IP+ISYEQL+PYIDRIANGD+SPILCT PITEFFIS+GT+GGE KLIP++E+E++
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 138 RRLSFFNYVIPRMKQLFPNS--HKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNN 197
           RRLSFF+YV+PRMKQLFPNS   KGK M F F K E  T++GI+A++ +TSLCKR++ N 
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLN- 180

Query: 198 IQSGGNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWIL 257
                NTSP D+ LCTDT QS Y QLLCGL QN+ V ++GALF STLI+VLKFLE+HW++
Sbjct: 181 -----NTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 258 LANDIRTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYI 317
           LA+DIR GT++ KI D SVRE+VMNIL PN QLADFIE EC+KG+WEGII RLWPNA YI
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 318 KAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYF 377
            AI +GSM+QY+P +NYYSN+LP++SDHYG SECFLGLNLDPLC P+  SYTL+P MAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGGSECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 378 EFLPIEREKANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPN 437
           EFLPI+R K++ E  +ELVDLVDVKLG EYELVITTY GLYRYCMGDILRV GFKNKAPN
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYCMGDILRVTGFKNKAPN 420

Query: 438 FSFVGRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGHYVLY 497
           FSFV RKN++L++D EKT+E+ELH+AVQEAARL+SPFGA I DYTS+AD++ IPGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 498 WEIHVKEESSIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGTFEKLVE 557
           WEIHVK   S I SSIYEDCC  +EELLN  YRY RS +KCINPLEIKIV+ GTFEKL+ 
Sbjct: 481 WEIHVKGGIS-IPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMA 540

Query: 558 RAVNQGASISQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWDPSGRHPLLS 609
           +A+N+GAS+SQYKTPR + ST IIELLD+ V++ +FS+ECPK D +  H LLS
Sbjct: 541 QALNRGASMSQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKVDTNALHRLLS 586

[Close]

BLAST of Sed0020777 vs. NCBI nr
Match: XP_022140778.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 861.3 bits (2224), Expect = 5.0e-246
Identity = 408/583 (69.98%), Postives = 481/583 (82.50%), Query Frame = 0


Query: 26  LEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPV 85
           L+EKDKQALQ +ED+T  A EIQR+ L EIL+TNA VEYLQQHGLNGSTDASTFKN IP+
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 86  ISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYVRRLSFFNY 145
           +SYE LKPYIDRI NGD+SPILC  PITEF+ STGT+GGEQKLIP  E+E+ R+LS  NY
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123

Query: 146 VIPRMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQ-SGGNTSP 205
           + PRMKQLFP  H GKEMFF F K ES TKAGI+A S STS  K  H NN++     TSP
Sbjct: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSP 183

Query: 206 IDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGT 265
            D+  CTD YQS Y QLLCGL QN+KV ++G+LF STLIY+ KFLENHW+LLANDIRTGT
Sbjct: 184 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 243

Query: 266 IDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMS 325
           I+ KI+DS VR+SVMNILKP+PQLADFIE EC+KG+WEGI+ RLWPN  +I A+ +GSMS
Sbjct: 244 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 303

Query: 326 QYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREK 385
            Y+P +NYY  +LPLI+  Y SSECF G+NLDPLCKPEEISYT +P +AYFEFLP++R  
Sbjct: 304 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 363

Query: 386 ANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVGRKNI 445
            +GE  QE VDLVDVKLGQEYE+V+TTY GLYRYC+GDI+RV GFKNKAPNF FVGRKN+
Sbjct: 364 DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNV 423

Query: 446 ILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGHYVLYWEIHVKEES 505
           IL++D EKT+E+ELHNAV +   L+S FGA +VDYTSYAD++T PGHYVLYWEI  KEE 
Sbjct: 424 ILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEI 483

Query: 506 SIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGTFEKLVERAVNQGASI 565
           SII SSIYEDCCL +EE L+ VYR  RS QK INPLEIKIVE+ TF+KL+ER V+QGASI
Sbjct: 484 SIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGASI 543

Query: 566 SQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWDPSGRHPLL 608
           SQYKTPR +KSTQIIE LD++V++N+FS++ PKWDP+  HP L
Sbjct: 544 SQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPHPNL 586

[Close]

BLAST of Sed0020777 vs. NCBI nr
Match: XP_022944596.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 854.0 bits (2205), Expect = 8.0e-244
Identity = 415/593 (69.98%), Postives = 498/593 (83.98%), Query Frame = 0


Query: 18  MSHEAENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDAS 77
           M+H  E   +E+DKQALQHIED+T +A +IQRQ L +IL+TNADVEYLQQHGL+GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 78  TFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYV 137
           TFKN IP+ISYEQL+PYIDRIANGD+SPILCT PITEFFIS+GT+GGE KLIP++E+E++
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 138 RRLSFFNYVIPRMKQLFPNS--HKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNN 197
           RRLSFF+YV+PRMKQLFPNS   KGK M F F K E  T++GI+A++ +TSLCKR++ N 
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLN- 180

Query: 198 IQSGGNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWIL 257
                NTSP D+ LCTDT QS Y QLLCGL QN+ V ++GALF STLI+VLKFLE+HW++
Sbjct: 181 -----NTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 258 LANDIRTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYI 317
           LA+DIR GT++ KI D SVRE+VMNIL PN QLADFIE EC+KG+WEGII RLWPNA YI
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 318 KAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYF 377
            AI +GSM+QY+P +NYYSN+LP++SDHYG+SECFLGLNLDPLC P+  SYTL+P MAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 378 EFLPIEREKANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPN 437
           EFLPI+R K++ E  +ELVDLVDVKLG EYELVITTY GLYRY MGDIL V GFKNKAPN
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPN 420

Query: 438 FSFVGRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGHYVLY 497
           FSFV RKN++L++D EKT+E+ELH+AVQEAARL+SPFGA I DYTS+AD++ IPGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 498 WEIHVKEESSIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGTFEKLVE 557
           WEIHVK   S I SSIYEDCC  +EELLN  YRY RS +KCINPLEIKIV+ GTFEKL+ 
Sbjct: 481 WEIHVKGGIS-IPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMA 540

Query: 558 RAVNQGASISQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWDPSGRHPLLS 609
           +A+N+GAS+SQYKTPR + ST IIELLD+ V+  +FS++CPK D    H LLS
Sbjct: 541 QALNRGASMSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALHRLLS 586

[Close]

BLAST of Sed0020777 vs. NCBI nr
Match: XP_038902067.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida])

HSP 1 Score: 813.5 bits (2100), Expect = 1.2e-231
Identity = 403/595 (67.73%), Postives = 491/595 (82.52%), Query Frame = 0


Query: 18  MSHEAENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDAS 77
           MSH  E+ L+EKDK+ALQHIED+TS A EIQRQ L EIL+TNA+VEYLQQHGL+GSTDAS
Sbjct: 1   MSHVVEHWLDEKDKEALQHIEDVTSKADEIQRQILTEILSTNANVEYLQQHGLHGSTDAS 60

Query: 78  TFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYV 137
           TFK  IP+ISYEQL PY+  IA+ D+SPILC+ PIT FF S+GT+GGE KLIP++E+E+V
Sbjct: 61  TFKKLIPLISYEQLTPYLALIAHDDDSPILCSNPITHFFTSSGTSGGETKLIPMYEEEFV 120

Query: 138 RRLSFFNYVIPRMKQLFPNSH--KGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNN 197
           RRLSFFNY++PRMKQLFPN +  KGK M F F K E  TKAGII  S  TSL KRS  +N
Sbjct: 121 RRLSFFNYMMPRMKQLFPNINWQKGKGMNFHFAKPEFKTKAGIIVRSIFTSLHKRS--SN 180

Query: 198 IQS---GGNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENH 257
           ++S   G NTSP D+ LCTD++QS Y QLLCGLYQN+ V +LGALF STLI+V KFLENH
Sbjct: 181 LKSKPIGNNTSPDDIILCTDSFQSLYCQLLCGLYQNDVVFRLGALFASTLIHVFKFLENH 240

Query: 258 WILLANDIRTGTIDPKIIDSSVRESVMNI-LKPNPQLADFIEIECNKGSWEGIIPRLWPN 317
           W++LANDI TG+I+PKI + SVRESVM I +KPNP++AD IE EC+KGSWEGII RLWPN
Sbjct: 241 WVVLANDILTGSINPKITNLSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPN 300

Query: 318 ANYIKAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPD 377
             YI AI +GSMSQY+PL+N+Y+NNLP++SDHYGSSECFLGLNLDPLC P EISYTL+P 
Sbjct: 301 TKYINAIVTGSMSQYIPLLNFYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPT 360

Query: 378 MAYFEFLPIEREKANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKN 437
           MAYFEFLPI+R   NGE  Q+LVDLVDVKLGQEYELVITT+ GLYRY MGDI+RV GF  
Sbjct: 361 MAYFEFLPIDRTNPNGEVTQQLVDLVDVKLGQEYELVITTFAGLYRYRMGDIIRVTGFTK 420

Query: 438 KAPNFSFVGRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGH 497
           KAPNFSFV RKN++L+++ EKT+E+ LH AV+E   ++ PFGATIVDYTSYAD++TIPGH
Sbjct: 421 KAPNFSFVCRKNVVLSLNNEKTDEAGLHKAVEEGGHVLKPFGATIVDYTSYADSSTIPGH 480

Query: 498 YVLYWEI----HVKEE-SSIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVE 557
           YVLY+E+    H K + S+ I SS+YEDCC  +EE LN +YR  RS +KCI+PLEIKIV+
Sbjct: 481 YVLYFELIFDEHNKAQISNSIPSSVYEDCCFAIEETLNSLYRLARSHEKCIDPLEIKIVK 540

Query: 558 SGTFEKLVERAVNQGASISQYKTPRSIKSTQ--IIELLDARVLSNHFSQECPKWD 600
           +GTFEKL++ A+N+GASI++YKTPR + S Q  II+LL++ ++SN+FSQ+ PK D
Sbjct: 541 AGTFEKLMQLAINRGASITRYKTPRCLNSKQIHIIQLLESNLVSNYFSQKSPKLD 593

[Close]

BLAST of Sed0020777 vs. NCBI nr
Match: XP_022140368.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia])

HSP 1 Score: 801.2 bits (2068), Expect = 6.2e-228
Identity = 389/578 (67.30%), Postives = 470/578 (81.31%), Query Frame = 0


Query: 22  AENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKN 81
           ++++L+ KDKQ L HIED TSNA EIQR+ + EIL+ NA VEYL+Q GL+GSTD STFK 
Sbjct: 2   SDDILDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKK 61

Query: 82  FIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYVRRLS 141
            IPV+SYEQLKPYIDRIA+GD+SPILC + IT+F ISTGT+GG QKLIP+F+ E +RRL+
Sbjct: 62  LIPVVSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLA 121

Query: 142 FFNYVIPRMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQ-SGG 201
              Y IP MKQLFP    GKEMFF FT+AE+ TK G++A   S+ L K S F++++    
Sbjct: 122 TNYYAIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLSSCLSKCSLFSSVEVLRA 181

Query: 202 NTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDI 261
            TSPID+  CTD YQS Y QLLCGL QN+ V ++GALF STLIY+ KFLENHW LL NDI
Sbjct: 182 YTSPIDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVNDI 241

Query: 262 RTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIAS 321
           RTGT+D KI D  VRESVM++LKPNP+LADFIE EC+KG+WEGI+ RLWPN  YI  + +
Sbjct: 242 RTGTVDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVVT 301

Query: 322 GSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPI 381
           GSMSQY+P +N+Y    P++S  Y SSECF G+NLDPLCKPEEISYT +P MAYFEFLP+
Sbjct: 302 GSMSQYIPQLNFYC-KCPIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLPV 361

Query: 382 EREKA-NGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFV 441
           +R  + NGE  QELVDLVDVKLGQEYE+V+TTY GLYRYCMGDILRV GFKNK PNFSF 
Sbjct: 362 DRANSNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSFA 421

Query: 442 GRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGHYVLYWEIH 501
            RKN+IL++D EKT+E +LH+AV EA+ L+SP GA IVDYTSYAD++T PGHYVLYWEI 
Sbjct: 422 CRKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEIQ 481

Query: 502 VKEESSIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGTFEKLVERAVN 561
            +EE+S I S++YEDCCL VEE L+ VYR  RS  K INPLEIKIVE+GTFEKL+E+AV+
Sbjct: 482 CREETSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAVD 541

Query: 562 QGASISQYKTPRSIKSTQIIELLDARVLSNHFSQECPK 598
           +GASISQYKTPR +KSTQ+IELLD++V+SN+FSQE PK
Sbjct: 542 RGASISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578

[Close]

BLAST of Sed0020777 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 2.5e-195
Identity = 336/601 (55.91%), Postives = 436/601 (72.55%), Query Frame = 0


Query: 15  PQKMSHEAENL-LEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGS 74
           P+K S E  +L L++K+KQ LQ IE++TSNA ++QRQ L EIL  NADVEYL++H LNG 
Sbjct: 5   PKKESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGR 64

Query: 75  TDASTFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFE 134
           TD  TFKN +PVI+YE ++P I+RIANGD SPIL ++PI+EF  S+GT+GGE+KL+P  E
Sbjct: 65  TDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIE 124

Query: 135 KEYVRRLSFFNYVIPRMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHF 194
           +E  RR   ++ ++P M Q  P    GK M+F F K+ES T  G+ A    TS  K SHF
Sbjct: 125 EELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHF 184

Query: 195 NNIQSG---GNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLE 254
                      TSP +  LC+D+YQS YSQ+LCGL Q+++V ++GA+F S  I  +KFLE
Sbjct: 185 KERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLE 244

Query: 255 NHWILLANDIRTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWP 314
            HWI L  DIRTGT+   I D SVRE+V  ILKP+P+LADF+E EC K SW+GII RLWP
Sbjct: 245 KHWIELVRDIRTGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWP 304

Query: 315 NANYIKAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVP 374
           N  Y+  I +G+MSQY+P ++YYSN LPL+   Y SSEC+ G+NL PLCKP E+SYTL+P
Sbjct: 305 NTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIP 364

Query: 375 DMAYFEFLPIEREKA-----------NGEARQELVDLVDVKLGQEYELVITTYGGLYRYC 434
            MAYFEFLP+ R                + +QELVDLVDVKLGQEYELV+TTY GL RY 
Sbjct: 365 SMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYR 424

Query: 435 MGDILRVKGFKNKAPNFSFVGRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDY 494
           +GD+LRV GFKNKAP FSF+ RKN++L++D +KT+E EL NAV+ A   + PF A++ +Y
Sbjct: 425 VGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEY 484

Query: 495 TSYADTTTIPGHYVLYWEIHVKEESSIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINP 554
           TSYADT++IPGHYVL+WE+ + + ++ I  S++EDCCL VEE  N VYR GR   K I P
Sbjct: 485 TSYADTSSIPGHYVLFWELCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGP 544

Query: 555 LEIKIVESGTFEKLVERAVNQGASISQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWD 601
           LEIKIVE GTF+KL++ A++ GASI+QYKTPR +K   IIELL++RV+ ++FS +CPKW 
Sbjct: 545 LEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWV 604

[Close]

BLAST of Sed0020777 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 3.2e-195
Identity = 329/593 (55.48%), Postives = 432/593 (72.85%), Query Frame = 0


Query: 22  AENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKN 81
           ++  L EK+K  LQ IED+T+NA ++QR+ L EIL+ NADVEYL++HGL G TD  TFK+
Sbjct: 13  SDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKH 72

Query: 82  FIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYVRRLS 141
            +PV++YE ++P I+RIANGD S +LC+ PI+EF  S+GT+GGE+KL+P  E+E  RR  
Sbjct: 73  IMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSL 132

Query: 142 FFNYVIPRMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSG-- 201
            ++ ++P M Q  P   KGK M+F F K+ES T  G+ A    TS  K SHF N      
Sbjct: 133 LYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPY 192

Query: 202 -GNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLAN 261
              TSP    LC+D+YQS YSQ+LCGL Q+++V ++GA+F S  I  +KFLE HW  LA 
Sbjct: 193 TNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELAR 252

Query: 262 DIRTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAI 321
           DIRTGT+  +I DSSVRE+V  ILKP+P+LADF+E EC K SW+GII RLWPN  Y+  I
Sbjct: 253 DIRTGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVI 312

Query: 322 ASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFL 381
            +G+MSQY+P ++YYSN LPL+   Y SSEC+ G+NL PLCKP E+SYTL+P+MAYFEFL
Sbjct: 313 VTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFL 372

Query: 382 PIEREKA-----------NGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVK 441
           P+ R                + +QELVDLVDVKLGQEYELV+TTY GLYRY +GD+L V 
Sbjct: 373 PVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVA 432

Query: 442 GFKNKAPNFSFVGRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTT 501
           GFKN AP FSF+ RKN++L++D +KT+E EL NAV+ A   + PF A++ +YTSYADT++
Sbjct: 433 GFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSS 492

Query: 502 IPGHYVLYWEIHVKEESSIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVES 561
           IPGHYVL+WE+ +   +  I  S++EDCCL +EE LN VYR GR   K I PLEIK+VES
Sbjct: 493 IPGHYVLFWELCLNGNTP-IPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVES 552

Query: 562 GTFEKLVERAVNQGASISQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWDP 601
           GTF+KL++ A++ GASI+QYKTPR +K   IIELL++RV+ ++FS +CPKW P
Sbjct: 553 GTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

[Close]

BLAST of Sed0020777 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 2.8e-175
Identity = 296/577 (51.30%), Postives = 402/577 (69.67%), Query Frame = 0


Query: 32  QALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTD-ASTFKNFIPVISYEQ 91
           +AL+ IE +T+NAG++QR+ L EILA NA  EYL+++G+ GS D    F+  +P+++YE 
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 92  LKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYVRRLSFFNYVIPRM 151
           L+P I RIANGD SPI   +PI+EF  S+GT+GGE+KL+P    E  RR   ++ ++P M
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141

Query: 152 KQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSG---GNTSPIDV 211
            Q      KGK M+  F KAES T  G+ A    TS  +   F +         TSP + 
Sbjct: 142 SQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEA 201

Query: 212 TLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGTIDP 271
            LC D+YQS Y+QLLCGL     V ++GA+F S  +  + FLE HW  L +DIRTG +DP
Sbjct: 202 ILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDP 261

Query: 272 KIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMSQYV 331
           +I D  VR++V  +L+ +P LAD IE EC + SWEGII RLWP   YI  I +G+MSQY+
Sbjct: 262 EITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYI 321

Query: 332 PLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREKANG 391
           P + +Y   LPL    Y SSEC+ GLNL+P+CKP +++YTL+P M Y+EFLP+    A  
Sbjct: 322 PTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATA 381

Query: 392 EA-RQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVGRKNIIL 451
           EA  ++LVDLVDVKLG EYELV+TTY GLYRY +GD+LRV GFKNKAP FSFV R+N+ L
Sbjct: 382 EASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVAL 441

Query: 452 TVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGHYVLYWEIHVKEESSI 511
           +VD +KT+E+ELH AV  A + ++PFGA++V+YTSYAD  TIPGHYVL+WE+  +  S+ 
Sbjct: 442 SVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWEL--RAGSTA 501

Query: 512 ISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGTFEKLVERAVNQGASISQ 571
           + +S++E+CCL VEE LN VYR GR+  + I PLEI++V  GTF+KL++ A+++GASI+Q
Sbjct: 502 VPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASINQ 561

Query: 572 YKTPRSIKSTQIIELLDARVLSNHFSQECPKWDPSGR 604
           YK PR ++   ++ELLDARV   +FS +CPKW P  +
Sbjct: 562 YKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNK 596

[Close]

BLAST of Sed0020777 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 2.7e-165
Identity = 288/591 (48.73%), Postives = 397/591 (67.17%), Query Frame = 0


Query: 31  KQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDAS--TFKNFIPVISY 90
           ++ L+HIE +T NAG+ QR+ L EILA NA  EYL++ G+ G    +   F+   P+++Y
Sbjct: 24  REKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGADEAFRRLAPLVTY 83

Query: 91  EQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYVRRLSFFNYVIP 150
           E + P + RIANGD SPIL  +P++EF  S+GT+GGE+KL+P  E+E  RR   ++ ++P
Sbjct: 84  EDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEEMERRSGLYSLLMP 143

Query: 151 RMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSGG---NTSPI 210
            M +  P   KGK M+  F K+E  T  G+ A    TS  +  +F           TSP 
Sbjct: 144 VMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLERPHDPYTVYTSPD 203

Query: 211 DVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGTI 270
           +  LC D YQS Y+QL+CGL     V ++GA+F S  +  ++FLE HW  L  DIR G +
Sbjct: 204 EAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKHWPSLCRDIRAGEL 263

Query: 271 DPKIIDSSVRESVMNILK-PNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMS 330
           D  + D +VR +V  +L+  +P LAD IE EC + SW+GII R+WP+  YI  I +G+M+
Sbjct: 264 DGGVTDPAVRGAVGRVLRGADPALADAIEAECARPSWQGIIRRVWPSTKYIDVIVTGAMA 323

Query: 331 QYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREK 390
           QY+P + +Y   LPL    Y SSEC+ GLNL+P+CKP E++YTL+P M YFEFLP+    
Sbjct: 324 QYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIPTMCYFEFLPV-NSG 383

Query: 391 ANGEARQE-----LVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFV 450
           AN  A  E     LVDLVDVKLG EYELV+TTY GLYRY +GD+LRV GFKN AP F+FV
Sbjct: 384 ANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNAAPMFAFV 443

Query: 451 GRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADT-TTIPGHYVLYWEI 510
            RKN+ L++D +KT+E+ELH AV EA + ++PFGA++V+YTSYADT TTIPGHYVL+WE+
Sbjct: 444 RRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTATTIPGHYVLFWEL 503

Query: 511 HVKEESSIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGTFEKLVERAV 570
                 + + +S++EDCCL VEE LN VYR  R+  + I PLEI++V  GTF+KL++ A+
Sbjct: 504 RSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVVADGTFDKLMDYAL 563

Query: 571 NQGASISQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWDPSGRHPLLSS 610
           ++GASI+QYK PR ++   ++ELLD RV + +FS +CPKW   G    +SS
Sbjct: 564 SRGASINQYKAPRCVRPGPVVELLDGRVQATYFSPKCPKWCAGGNKQWISS 613

[Close]

BLAST of Sed0020777 vs. ExPASy Swiss-Prot
Match: Q9LQ68 (Indole-3-acetic acid-amido synthetase GH3.4 OS=Arabidopsis thaliana OX=3702 GN=GH3.4 PE=1 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 3.5e-165
Identity = 276/580 (47.59%), Postives = 401/580 (69.14%), Query Frame = 0


Query: 28  EKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPVIS 87
           E + +AL+ IE+IT N   +Q + L EIL+ N++ EYL++  LNG+ D  +FK+ +PV+ 
Sbjct: 18  ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPVVI 77

Query: 88  YEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYVRRLSFFNYVI 147
           YE LK  I RI+NGD SPIL + PITEF  S+GT+ GE+KL+P  E++  RR    N ++
Sbjct: 78  YEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGNLLM 137

Query: 148 PRMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSGG-NTSPID 207
           P M    P   KGK ++F F K+ESTT  G+ A    TS  K  +F    S    TSP +
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSDSVYTSPKE 197

Query: 208 VTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGTID 267
             LC D+ QS Y+Q+LCGL    +V++LGA+F S L+  + FL+N+W  L+ DI TGT+ 
Sbjct: 198 AILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDISTGTLS 257

Query: 268 PKIIDSSVRESVMNIL-KPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMSQ 327
            KI D +++  + NIL KP+ +LA+F+   C++ +WEGII ++WPN  Y+  I +G+M++
Sbjct: 258 SKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTGAMAE 317

Query: 328 YVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREKA 387
           Y+P++ YYS  LP+ S  Y SSE + G+NL+P+CKP E+SYT+ P+MAYFEFLP   +  
Sbjct: 318 YIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHNHDGD 377

Query: 388 NGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVGRKNII 447
            G     LV+L DV++G+EYELVITTY GLYRY +GDILRV GF N AP F F+ R+N++
Sbjct: 378 GGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRENVL 437

Query: 448 LTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGHYVLYWEIHVKEESS 507
           L+++ +KT+E++L  AV+ A+RL++  G  +++YTSYADT TIPGHYV+YWE+  +++S+
Sbjct: 438 LSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELLSRDQSN 497

Query: 508 II-SSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGTFEKLVERAVNQGASI 567
            + S  +   CCL +EE LN VYR  R   K I PLEI++V++GTFE+L++ ++++G+SI
Sbjct: 498 ALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFSISRGSSI 557

Query: 568 SQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWDPSGRH 605
           +QYK PR +  T I++LLD+RV+S HFS   P W P  RH
Sbjct: 558 NQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERRH 597

[Close]

BLAST of Sed0020777 vs. ExPASy TrEMBL
Match: A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 2.4e-246
Identity = 408/583 (69.98%), Postives = 481/583 (82.50%), Query Frame = 0


Query: 26  LEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPV 85
           L+EKDKQALQ +ED+T  A EIQR+ L EIL+TNA VEYLQQHGLNGSTDASTFKN IP+
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 86  ISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYVRRLSFFNY 145
           +SYE LKPYIDRI NGD+SPILC  PITEF+ STGT+GGEQKLIP  E+E+ R+LS  NY
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123

Query: 146 VIPRMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQ-SGGNTSP 205
           + PRMKQLFP  H GKEMFF F K ES TKAGI+A S STS  K  H NN++     TSP
Sbjct: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSP 183

Query: 206 IDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGT 265
            D+  CTD YQS Y QLLCGL QN+KV ++G+LF STLIY+ KFLENHW+LLANDIRTGT
Sbjct: 184 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 243

Query: 266 IDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMS 325
           I+ KI+DS VR+SVMNILKP+PQLADFIE EC+KG+WEGI+ RLWPN  +I A+ +GSMS
Sbjct: 244 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 303

Query: 326 QYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREK 385
            Y+P +NYY  +LPLI+  Y SSECF G+NLDPLCKPEEISYT +P +AYFEFLP++R  
Sbjct: 304 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 363

Query: 386 ANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVGRKNI 445
            +GE  QE VDLVDVKLGQEYE+V+TTY GLYRYC+GDI+RV GFKNKAPNF FVGRKN+
Sbjct: 364 DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNV 423

Query: 446 ILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGHYVLYWEIHVKEES 505
           IL++D EKT+E+ELHNAV +   L+S FGA +VDYTSYAD++T PGHYVLYWEI  KEE 
Sbjct: 424 ILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEI 483

Query: 506 SIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGTFEKLVERAVNQGASI 565
           SII SSIYEDCCL +EE L+ VYR  RS QK INPLEIKIVE+ TF+KL+ER V+QGASI
Sbjct: 484 SIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGASI 543

Query: 566 SQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWDPSGRHPLL 608
           SQYKTPR +KSTQIIE LD++V++N+FS++ PKWDP+  HP L
Sbjct: 544 SQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPHPNL 586

[Close]

BLAST of Sed0020777 vs. ExPASy TrEMBL
Match: A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)

HSP 1 Score: 854.0 bits (2205), Expect = 3.9e-244
Identity = 415/593 (69.98%), Postives = 498/593 (83.98%), Query Frame = 0


Query: 18  MSHEAENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDAS 77
           M+H  E   +E+DKQALQHIED+T +A +IQRQ L +IL+TNADVEYLQQHGL+GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 78  TFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYV 137
           TFKN IP+ISYEQL+PYIDRIANGD+SPILCT PITEFFIS+GT+GGE KLIP++E+E++
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 138 RRLSFFNYVIPRMKQLFPNS--HKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNN 197
           RRLSFF+YV+PRMKQLFPNS   KGK M F F K E  T++GI+A++ +TSLCKR++ N 
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLN- 180

Query: 198 IQSGGNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWIL 257
                NTSP D+ LCTDT QS Y QLLCGL QN+ V ++GALF STLI+VLKFLE+HW++
Sbjct: 181 -----NTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 258 LANDIRTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYI 317
           LA+DIR GT++ KI D SVRE+VMNIL PN QLADFIE EC+KG+WEGII RLWPNA YI
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 318 KAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYF 377
            AI +GSM+QY+P +NYYSN+LP++SDHYG+SECFLGLNLDPLC P+  SYTL+P MAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 378 EFLPIEREKANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPN 437
           EFLPI+R K++ E  +ELVDLVDVKLG EYELVITTY GLYRY MGDIL V GFKNKAPN
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPN 420

Query: 438 FSFVGRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGHYVLY 497
           FSFV RKN++L++D EKT+E+ELH+AVQEAARL+SPFGA I DYTS+AD++ IPGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 498 WEIHVKEESSIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGTFEKLVE 557
           WEIHVK   S I SSIYEDCC  +EELLN  YRY RS +KCINPLEIKIV+ GTFEKL+ 
Sbjct: 481 WEIHVKGGIS-IPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMA 540

Query: 558 RAVNQGASISQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWDPSGRHPLLS 609
           +A+N+GAS+SQYKTPR + ST IIELLD+ V+  +FS++CPK D    H LLS
Sbjct: 541 QALNRGASMSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALHRLLS 586

[Close]

BLAST of Sed0020777 vs. ExPASy TrEMBL
Match: A0A6J1CHU9 (indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 GN=LOC111011061 PE=3 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 3.0e-228
Identity = 389/578 (67.30%), Postives = 470/578 (81.31%), Query Frame = 0


Query: 22  AENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKN 81
           ++++L+ KDKQ L HIED TSNA EIQR+ + EIL+ NA VEYL+Q GL+GSTD STFK 
Sbjct: 2   SDDILDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKK 61

Query: 82  FIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYVRRLS 141
            IPV+SYEQLKPYIDRIA+GD+SPILC + IT+F ISTGT+GG QKLIP+F+ E +RRL+
Sbjct: 62  LIPVVSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLA 121

Query: 142 FFNYVIPRMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQ-SGG 201
              Y IP MKQLFP    GKEMFF FT+AE+ TK G++A   S+ L K S F++++    
Sbjct: 122 TNYYAIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLSSCLSKCSLFSSVEVLRA 181

Query: 202 NTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDI 261
            TSPID+  CTD YQS Y QLLCGL QN+ V ++GALF STLIY+ KFLENHW LL NDI
Sbjct: 182 YTSPIDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVNDI 241

Query: 262 RTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIAS 321
           RTGT+D KI D  VRESVM++LKPNP+LADFIE EC+KG+WEGI+ RLWPN  YI  + +
Sbjct: 242 RTGTVDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVVT 301

Query: 322 GSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPI 381
           GSMSQY+P +N+Y    P++S  Y SSECF G+NLDPLCKPEEISYT +P MAYFEFLP+
Sbjct: 302 GSMSQYIPQLNFYC-KCPIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLPV 361

Query: 382 EREKA-NGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFV 441
           +R  + NGE  QELVDLVDVKLGQEYE+V+TTY GLYRYCMGDILRV GFKNK PNFSF 
Sbjct: 362 DRANSNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSFA 421

Query: 442 GRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGHYVLYWEIH 501
            RKN+IL++D EKT+E +LH+AV EA+ L+SP GA IVDYTSYAD++T PGHYVLYWEI 
Sbjct: 422 CRKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEIQ 481

Query: 502 VKEESSIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGTFEKLVERAVN 561
            +EE+S I S++YEDCCL VEE L+ VYR  RS  K INPLEIKIVE+GTFEKL+E+AV+
Sbjct: 482 CREETSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAVD 541

Query: 562 QGASISQYKTPRSIKSTQIIELLDARVLSNHFSQECPK 598
           +GASISQYKTPR +KSTQ+IELLD++V+SN+FSQE PK
Sbjct: 542 RGASISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578

[Close]

BLAST of Sed0020777 vs. ExPASy TrEMBL
Match: A0A5A7V3L5 (Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G00190 PE=3 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 3.4e-224
Identity = 385/589 (65.37%), Postives = 476/589 (80.81%), Query Frame = 0


Query: 18  MSHEAENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDAS 77
           MS+  E  L+EKDK+ALQ+IEDIT+ A EIQRQ L EIL+ NA+VEYLQ+HGL+ STD+S
Sbjct: 1   MSNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSS 60

Query: 78  TFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYV 137
           TFK  IP++ Y+QL+PYI RIA+GD+S ILC+ PITEFF S+GT+G E KLIP++E+E+V
Sbjct: 61  TFKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFV 120

Query: 138 RRLSFFNYVIPRMKQLFPNS--HKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKR-SHFN 197
           RRLSFF+Y+ PRMKQLFPN   HK K + F F K E  TK GI+  S  T+L KR S+  
Sbjct: 121 RRLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLR 180

Query: 198 NIQSGGNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWI 257
           ++ SG NTSP D+ LCTD+YQS Y QLLCGLYQN+ V ++ ALF STLI+V KFLENHWI
Sbjct: 181 SMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWI 240

Query: 258 LLANDIRTGTIDPKIIDSSVRESVMNI-LKPNPQLADFIEIECNKGSWEGIIPRLWPNAN 317
            LA DIRT TI+PKI  SSVRESVM I +KPNP++AD IE EC KG WEGII RLWPNA 
Sbjct: 241 DLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAK 300

Query: 318 YIKAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMA 377
           YI A+ +GSMSQY+PL+NYY+NNLP++SDHYGSSECFLGLNLDPLC P+E+SYTL+P MA
Sbjct: 301 YINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMA 360

Query: 378 YFEFLPIEREKANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKA 437
           YFEFLPI+     GE  Q LVDLVDVKLG EYELVITT+ GLYRYCMGD++RV GFKNKA
Sbjct: 361 YFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKA 420

Query: 438 PNFSFVGRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGHYV 497
           PNFSFV RKN++L +  EKT+E+ LH AV+E  R++  FGATIVDYT+YAD +TIPGHYV
Sbjct: 421 PNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYV 480

Query: 498 LYWEI---HVKEES-SIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGT 557
           LYWE+    +KE++ + I  S++EDCCL +E  LN +YR  RS +KCINPLEIKIV++GT
Sbjct: 481 LYWELTLDDLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKAGT 540

Query: 558 FEKLVERAVNQGASISQYKTPRSIKSTQ--IIELLDARVLSNHFSQECP 597
           FEKL++ A+N+GASI+QYKTPR + S+Q  II+LL++ ++SN+FS+  P
Sbjct: 541 FEKLMQLALNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYP 589

[Close]

BLAST of Sed0020777 vs. ExPASy TrEMBL
Match: A0A1S3BJT8 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo OX=3656 GN=LOC103490488 PE=3 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 3.4e-224
Identity = 385/589 (65.37%), Postives = 476/589 (80.81%), Query Frame = 0


Query: 18  MSHEAENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDAS 77
           MS+  E  L+EKDK+ALQ+IEDIT+ A EIQRQ L EIL+ NA+VEYLQ+HGL+ STD+S
Sbjct: 1   MSNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSS 60

Query: 78  TFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYV 137
           TFK  IP++ Y+QL+PYI RIA+GD+S ILC+ PITEFF S+GT+G E KLIP++E+E+V
Sbjct: 61  TFKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFV 120

Query: 138 RRLSFFNYVIPRMKQLFPNS--HKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKR-SHFN 197
           RRLSFF+Y+ PRMKQLFPN   HK K + F F K E  TK GI+  S  T+L KR S+  
Sbjct: 121 RRLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLR 180

Query: 198 NIQSGGNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWI 257
           ++ SG NTSP D+ LCTD+YQS Y QLLCGLYQN+ V ++ ALF STLI+V KFLENHWI
Sbjct: 181 SMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWI 240

Query: 258 LLANDIRTGTIDPKIIDSSVRESVMNI-LKPNPQLADFIEIECNKGSWEGIIPRLWPNAN 317
            LA DIRT TI+PKI  SSVRESVM I +KPNP++AD IE EC KG WEGII RLWPNA 
Sbjct: 241 DLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAK 300

Query: 318 YIKAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMA 377
           YI A+ +GSMSQY+PL+NYY+NNLP++SDHYGSSECFLGLNLDPLC P+E+SYTL+P MA
Sbjct: 301 YINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMA 360

Query: 378 YFEFLPIEREKANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKA 437
           YFEFLPI+     GE  Q LVDLVDVKLG EYELVITT+ GLYRYCMGD++RV GFKNKA
Sbjct: 361 YFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKA 420

Query: 438 PNFSFVGRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGHYV 497
           PNFSFV RKN++L +  EKT+E+ LH AV+E  R++  FGATIVDYT+YAD +TIPGHYV
Sbjct: 421 PNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYV 480

Query: 498 LYWEI---HVKEES-SIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGT 557
           LYWE+    +KE++ + I  S++EDCCL +E  LN +YR  RS +KCINPLEIKIV++GT
Sbjct: 481 LYWELTLDDLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKAGT 540

Query: 558 FEKLVERAVNQGASISQYKTPRSIKSTQ--IIELLDARVLSNHFSQECP 597
           FEKL++ A+N+GASI+QYKTPR + S+Q  II+LL++ ++SN+FS+  P
Sbjct: 541 FEKLMQLALNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYP 589

[Close]

BLAST of Sed0020777 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 683.3 bits (1762), Expect = 1.7e-196
Identity = 336/601 (55.91%), Postives = 436/601 (72.55%), Query Frame = 0


Query: 15  PQKMSHEAENL-LEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGS 74
           P+K S E  +L L++K+KQ LQ IE++TSNA ++QRQ L EIL  NADVEYL++H LNG 
Sbjct: 5   PKKESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGR 64

Query: 75  TDASTFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFE 134
           TD  TFKN +PVI+YE ++P I+RIANGD SPIL ++PI+EF  S+GT+GGE+KL+P  E
Sbjct: 65  TDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIE 124

Query: 135 KEYVRRLSFFNYVIPRMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHF 194
           +E  RR   ++ ++P M Q  P    GK M+F F K+ES T  G+ A    TS  K SHF
Sbjct: 125 EELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHF 184

Query: 195 NNIQSG---GNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLE 254
                      TSP +  LC+D+YQS YSQ+LCGL Q+++V ++GA+F S  I  +KFLE
Sbjct: 185 KERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLE 244

Query: 255 NHWILLANDIRTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWP 314
            HWI L  DIRTGT+   I D SVRE+V  ILKP+P+LADF+E EC K SW+GII RLWP
Sbjct: 245 KHWIELVRDIRTGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWP 304

Query: 315 NANYIKAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVP 374
           N  Y+  I +G+MSQY+P ++YYSN LPL+   Y SSEC+ G+NL PLCKP E+SYTL+P
Sbjct: 305 NTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIP 364

Query: 375 DMAYFEFLPIEREKA-----------NGEARQELVDLVDVKLGQEYELVITTYGGLYRYC 434
            MAYFEFLP+ R                + +QELVDLVDVKLGQEYELV+TTY GL RY 
Sbjct: 365 SMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYR 424

Query: 435 MGDILRVKGFKNKAPNFSFVGRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDY 494
           +GD+LRV GFKNKAP FSF+ RKN++L++D +KT+E EL NAV+ A   + PF A++ +Y
Sbjct: 425 VGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEY 484

Query: 495 TSYADTTTIPGHYVLYWEIHVKEESSIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINP 554
           TSYADT++IPGHYVL+WE+ + + ++ I  S++EDCCL VEE  N VYR GR   K I P
Sbjct: 485 TSYADTSSIPGHYVLFWELCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGP 544

Query: 555 LEIKIVESGTFEKLVERAVNQGASISQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWD 601
           LEIKIVE GTF+KL++ A++ GASI+QYKTPR +K   IIELL++RV+ ++FS +CPKW 
Sbjct: 545 LEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWV 604

[Close]

BLAST of Sed0020777 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 682.9 bits (1761), Expect = 2.3e-196
Identity = 329/593 (55.48%), Postives = 432/593 (72.85%), Query Frame = 0


Query: 22  AENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKN 81
           ++  L EK+K  LQ IED+T+NA ++QR+ L EIL+ NADVEYL++HGL G TD  TFK+
Sbjct: 13  SDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKH 72

Query: 82  FIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYVRRLS 141
            +PV++YE ++P I+RIANGD S +LC+ PI+EF  S+GT+GGE+KL+P  E+E  RR  
Sbjct: 73  IMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSL 132

Query: 142 FFNYVIPRMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSG-- 201
            ++ ++P M Q  P   KGK M+F F K+ES T  G+ A    TS  K SHF N      
Sbjct: 133 LYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPY 192

Query: 202 -GNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLAN 261
              TSP    LC+D+YQS YSQ+LCGL Q+++V ++GA+F S  I  +KFLE HW  LA 
Sbjct: 193 TNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELAR 252

Query: 262 DIRTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAI 321
           DIRTGT+  +I DSSVRE+V  ILKP+P+LADF+E EC K SW+GII RLWPN  Y+  I
Sbjct: 253 DIRTGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVI 312

Query: 322 ASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFL 381
            +G+MSQY+P ++YYSN LPL+   Y SSEC+ G+NL PLCKP E+SYTL+P+MAYFEFL
Sbjct: 313 VTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFL 372

Query: 382 PIEREKA-----------NGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVK 441
           P+ R                + +QELVDLVDVKLGQEYELV+TTY GLYRY +GD+L V 
Sbjct: 373 PVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVA 432

Query: 442 GFKNKAPNFSFVGRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTT 501
           GFKN AP FSF+ RKN++L++D +KT+E EL NAV+ A   + PF A++ +YTSYADT++
Sbjct: 433 GFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSS 492

Query: 502 IPGHYVLYWEIHVKEESSIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVES 561
           IPGHYVL+WE+ +   +  I  S++EDCCL +EE LN VYR GR   K I PLEIK+VES
Sbjct: 493 IPGHYVLFWELCLNGNTP-IPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVES 552

Query: 562 GTFEKLVERAVNQGASISQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWDP 601
           GTF+KL++ A++ GASI+QYKTPR +K   IIELL++RV+ ++FS +CPKW P
Sbjct: 553 GTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

[Close]

BLAST of Sed0020777 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 583.2 bits (1502), Expect = 2.5e-166
Identity = 276/580 (47.59%), Postives = 401/580 (69.14%), Query Frame = 0


Query: 28  EKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPVIS 87
           E + +AL+ IE+IT N   +Q + L EIL+ N++ EYL++  LNG+ D  +FK+ +PV+ 
Sbjct: 18  ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPVVI 77

Query: 88  YEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYVRRLSFFNYVI 147
           YE LK  I RI+NGD SPIL + PITEF  S+GT+ GE+KL+P  E++  RR    N ++
Sbjct: 78  YEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGNLLM 137

Query: 148 PRMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSGG-NTSPID 207
           P M    P   KGK ++F F K+ESTT  G+ A    TS  K  +F    S    TSP +
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSDSVYTSPKE 197

Query: 208 VTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGTID 267
             LC D+ QS Y+Q+LCGL    +V++LGA+F S L+  + FL+N+W  L+ DI TGT+ 
Sbjct: 198 AILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDISTGTLS 257

Query: 268 PKIIDSSVRESVMNIL-KPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMSQ 327
            KI D +++  + NIL KP+ +LA+F+   C++ +WEGII ++WPN  Y+  I +G+M++
Sbjct: 258 SKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTGAMAE 317

Query: 328 YVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREKA 387
           Y+P++ YYS  LP+ S  Y SSE + G+NL+P+CKP E+SYT+ P+MAYFEFLP   +  
Sbjct: 318 YIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHNHDGD 377

Query: 388 NGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVGRKNII 447
            G     LV+L DV++G+EYELVITTY GLYRY +GDILRV GF N AP F F+ R+N++
Sbjct: 378 GGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRENVL 437

Query: 448 LTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGHYVLYWEIHVKEESS 507
           L+++ +KT+E++L  AV+ A+RL++  G  +++YTSYADT TIPGHYV+YWE+  +++S+
Sbjct: 438 LSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELLSRDQSN 497

Query: 508 II-SSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGTFEKLVERAVNQGASI 567
            + S  +   CCL +EE LN VYR  R   K I PLEI++V++GTFE+L++ ++++G+SI
Sbjct: 498 ALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFSISRGSSI 557

Query: 568 SQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWDPSGRH 605
           +QYK PR +  T I++LLD+RV+S HFS   P W P  RH
Sbjct: 558 NQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERRH 597

[Close]

BLAST of Sed0020777 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 581.6 bits (1498), Expect = 7.2e-166
Identity = 286/580 (49.31%), Postives = 390/580 (67.24%), Query Frame = 0


Query: 28  EKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPVIS 87
           EKD +AL+ IE++T NA  +Q   LAEILA NAD EYL++  L G+TD  TFK  IPVI+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 88  YEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYVRRLSFFNYVI 147
           YE L+P I RIA+GD SPIL   PI+EF  S+GT+ GE+KL+P  ++E  RR   ++ ++
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 148 PRMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNN---IQSGGNTSP 207
           P M    P   KGK M+F F K+E+ T  G+ A    TS  K  HF +         TSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 208 IDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGT 267
            +  LC D++QS Y+Q+LCGL     V ++GA+F S L+  ++FL+ HW   A+DI  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 268 IDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMS 327
           +D +I D S+R+ +  ILKP+P LA+FI  EC   +WE II R+WPN  Y+  I +G+M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316

Query: 328 QYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREK 387
           QY+P + YYS  LP+    Y SSEC+ GLNL+P+ KP E+SYT++P+MAYFEF+P+   K
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGTK 376

Query: 388 ANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVGRKNI 447
           A        V+LVDV +G+EYELV+TTY GL RY +GDILRV GF N AP F FV RKN+
Sbjct: 377 A--------VELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNV 436

Query: 448 ILTVDYEKTEESELHNAVQEAARLI-SPFGATIVDYTSYADTTTIPGHYVLYWEIHVKEE 507
           +L++D +KT+ESEL  AV+ A+ ++    G+ + +YTSYADT+TIPGHYVLYWE+ V++ 
Sbjct: 437 LLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDG 496

Query: 508 SSIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGTFEKLVERAVNQGAS 567
           +   S      CCL +EE LN VYR  R     + PLEI++V +GTFE+L++ A+++GAS
Sbjct: 497 ARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGAS 556

Query: 568 ISQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWDPSGR 604
           I+QYK PR +  T I+ELLD+RV+S HFS   P W P  R
Sbjct: 557 INQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERR 588

[Close]

BLAST of Sed0020777 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 573.9 bits (1478), Expect = 1.5e-163
Identity = 279/586 (47.61%), Postives = 397/586 (67.75%), Query Frame = 0


Query: 28  EKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPVIS 87
           EKD +AL+ IE++T N   +Q + L EIL  N++ EYL++  L+G  D  TFK+ +PV++
Sbjct: 18  EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77

Query: 88  YEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEYVRRLSFFNYVI 147
           YE LKP I RI+NGD SPIL + PITEF  S+GT+ GE+KL+P  E++  RR   ++ ++
Sbjct: 78  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137

Query: 148 PRMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNN---IQSGGNTSP 207
           P M    P   KGK ++F F K+ES T  G+ A    TS  K  HF           TSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197

Query: 208 IDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGT 267
            +  LC+D+ QS Y+Q+LCGL    +V +LGA+F S L+  + FL+N+W  LA DI TGT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257

Query: 268 IDPKIIDSSVRESVMNIL-KPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSM 327
           +  +I D +++  +  IL KP+ +LA+F+   C++ +WEGII ++WPN  Y+  I +G+M
Sbjct: 258 LSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAM 317

Query: 328 SQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIERE 387
           +QY+P + YYS  LP+    Y SSE + G+NL P+CKP E+SYT++P+MAYFEFLP    
Sbjct: 318 AQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP-HNH 377

Query: 388 KANGEA-----RQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSF 447
             +G A        LV+L +V++G+EYELVITTY GLYRY +GDILRV GF N AP F F
Sbjct: 378 DGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKF 437

Query: 448 VGRKNIILTVDYEKTEESELHNAVQEAARLISPFGATIVDYTSYADTTTIPGHYVLYWEI 507
           + RKN++L+V+ +KT+E+EL  AV+ A+RL +  G  +++YTSYA+T TIPGHYV+YWE+
Sbjct: 438 IRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWEL 497

Query: 508 HVKEES-SIISSSIYEDCCLIVEELLNPVYRYGRSQQKCINPLEIKIVESGTFEKLVERA 567
             +++S +++S  +   CCL +EE LN VYR  R   K I PLEI++V +GTFE+L++ A
Sbjct: 498 LGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELMDYA 557

Query: 568 VNQGASISQYKTPRSIKSTQIIELLDARVLSNHFSQECPKWDPSGR 604
           +++GASI+QYK PR +  T I+ELLD+RV+S HFS   P W P  R
Sbjct: 558 ISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPERR 602

The following BLAST results are available for this feature:

BLAST of Sed0020777 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6570863.1	7.8e-247	70.49	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022140778.1	5.0e-246	69.98	indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia]	[more]
XP_022944596.1	8.0e-244	69.98	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
XP_038902067.1	1.2e-231	67.73	indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida]	[more]
XP_022140368.1	6.2e-228	67.30	indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia]	[more]

BLAST of Sed0020777 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81829	2.5e-195	55.91	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q9LSQ4	3.2e-195	55.48	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8LQM5	2.8e-175	51.30	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]
Q60EJ6	2.7e-165	48.73	Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo...	[more]
Q9LQ68	3.5e-165	47.59	Indole-3-acetic acid-amido synthetase GH3.4 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]

BLAST of Sed0020777 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CIS9	2.4e-246	69.98	indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1FUU5	3.9e-244	69.98	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1CHU9	3.0e-228	67.30	indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 ...	[more]
A0A5A7V3L5	3.4e-224	65.37	Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BJT8	3.4e-224	65.37	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]

BLAST of Sed0020777 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27260.1	1.7e-196	55.91	Auxin-responsive GH3 family protein	[more]
AT5G54510.1	2.3e-196	55.48	Auxin-responsive GH3 family protein	[more]
AT1G59500.1	2.5e-166	47.59	Auxin-responsive GH3 family protein	[more]
AT2G14960.1	7.2e-166	49.31	Auxin-responsive GH3 family protein	[more]
AT4G37390.1	1.5e-163	47.61	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 37..576 e-value: 2.9E-181 score: 603.9
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 22..602
None	No IPR available	PANTHER	PTHR31901:SF63	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE	coord: 22..602

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0020777.1	Sed0020777.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity