Sed0020701 (gene) Chayote v1

BLAST of Sed0020701 vs. NCBI nr
Match: XP_038897831.1 (autophagy-related protein 18f [Benincasa hispida])

HSP 1 Score: 1350.5 bits (3494), Expect = 0.0e+00
Identity = 709/887 (79.93%), Postives = 776/887 (87.49%), Query Frame = 0

Query: 1   MRN-GGVKRQGTIARSGRAHGFISSSFRTFSSYVKIVSSGASTVARSAASVASSFVDKDD 60
           MRN G +K+QGT+ARSGRAH F+SSSFR FSSY+KIVS+GASTVARSAASVASS VDKDD
Sbjct: 27  MRNDGALKQQGTVARSGRAHEFLSSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDD 86

Query: 61  ESNGSQVNWAGFDKLEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQ 120
           E+NG QVNWAGFDKLEWD++ +R VLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSY+Q
Sbjct: 87  EANGFQVNWAGFDKLEWDDNAIRQVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 146

Query: 121 VLPRPIPSMRSGDKFAESRPLLVLSAYGSIAAGFNIQEQLATSGNAAITKGQELADDNLM 180
           VLPRPIPSMR GDKFAESRPLLVLSAYGSIAA FN+Q++LA+SGNAAI KGQEL D NLM
Sbjct: 147 VLPRPIPSMRPGDKFAESRPLLVLSAYGSIAAAFNVQDRLASSGNAAIPKGQELVDGNLM 206

Query: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNP 240
           PTFVRFYSLKSQTYVHELKFRS VYSVRCSPLVVAISLATQ+HCINATTLE EHII TNP
Sbjct: 207 PTFVRFYSLKSQTYVHELKFRSAVYSVRCSPLVVAISLATQVHCINATTLEKEHIILTNP 266

Query: 241 IVSGFPGSGEGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKP-ASLSHSSSNGSVV 300
           +VSG PGSG GIGYGPLAVGPRWLAYSG++IM SNTGRVIPQHLKP AS SHS+SNGS+V
Sbjct: 267 VVSGIPGSGGGIGYGPLAVGPRWLAYSGNLIMVSNTGRVIPQHLKPSASFSHSASNGSLV 326

Query: 301 AHYAKESSKQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAAD 360
           AHYAKESSKQLAAG+VTLGDKGIKKLSRYYS+LLPESNNS QSG QGLKGIG LNGHAAD
Sbjct: 327 AHYAKESSKQLAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGGQGLKGIGALNGHAAD 386

Query: 361 ADSVGMVIVKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSG 420
           ADSVGMVIVKDIISKVVITQF+AHKSPISALCFDPSGTILVTASV GH+INVFKIMPSS 
Sbjct: 387 ADSVGMVIVKDIISKVVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSS 446

Query: 421 LNSPASGTGASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQV 480
            NS  S T ASY H+Y LQRGFTNAVIQDISFSY+SNWIM+SSSRGTSHLFAINP+GGQV
Sbjct: 447 SNSSESSTSASYSHLYRLQRGFTNAVIQDISFSYDSNWIMVSSSRGTSHLFAINPAGGQV 506

Query: 481 NFPSADTSSITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHGSNG 540
           NFPSAD SSITRNGG VV AR+T+RR+D GLHMPSKQN+CA   P+TLSAV+RIHHG+NG
Sbjct: 507 NFPSADISSITRNGGPVVPARQTVRRVDYGLHMPSKQNQCATGSPLTLSAVTRIHHGNNG 566

Query: 541 WRGTVTSAAAAATGRMGSTSGAIASAFHDCKGNALHMSHGSSDARYHILVFSPSGSMIQY 600
           WRGTV+SAAAAATGRMG+ SGAIASAFH+CKGNA+H+  GSS+ RYHILVFSPSGSMIQY
Sbjct: 567 WRGTVSSAAAAATGRMGTVSGAIASAFHECKGNAVHVDSGSSEVRYHILVFSPSGSMIQY 626

Query: 601 VLRVDSDSTIVLPKSSTAHELEPGLDGRLVVEAIQKWNIFQKQNRRAQDNSSDIYGDNGA 660
            LRV  DST VLP+SS A EL P LD RLVVEAIQKWNI QKQNRRAQDNS DIYGDNG 
Sbjct: 627 ALRVGLDSTKVLPRSSMALELVPELDARLVVEAIQKWNISQKQNRRAQDNSVDIYGDNGG 686

Query: 661 LDFHHNYSGEMNGKPIFHEASGTVLKAKTCREEQHHLYISEAELQMHGARSPLWMKPEIY 720
            D + NY  EMN  P+  EA+G V KAKTCREEQ+HLYISEAELQMHG  +PLW K EIY
Sbjct: 687 FDCNKNYCEEMNENPVL-EATGNVFKAKTCREEQYHLYISEAELQMHGTHTPLWTKSEIY 746

Query: 721 FQVMTTFAIKGDEIDHPGEIEIERIPTRMVEARSKGLIPVFDCLQTPKISQTWSLNRNKD 780
           FQVM    +  DE +HPGE+EIERIPTRM+EARSK L+PVFD LQ+ KISQ   LN N D
Sbjct: 747 FQVMAKDGVHVDEFNHPGELEIERIPTRMIEARSKDLVPVFDYLQSSKISQPRYLNSNSD 806

Query: 781 ----HQKSGLFENGRQS-RNDSSPPGSVINNEPDRNGSHVHKMVTEETKGIVNDYEGSRS 840
               HQKSG FENGRQS R+D+SP GSV+  +P+    H +KMVT ETKGIVN+YE  RS
Sbjct: 807 QLLLHQKSGQFENGRQSWRSDASPHGSVV-GKPEVTDRHGYKMVT-ETKGIVNEYECPRS 866

Query: 841 QTQHNNVNNCTESLSMESTTQAKSVNN-HENGMEVENHFEDMGDVFD 880
           QT  NNVNNCTESLSMES  Q KSVNN H++G+ +ENHFED+GD F+
Sbjct: 867 QTHLNNVNNCTESLSMES--QPKSVNNHHKSGLRLENHFEDLGDEFE 908

BLAST of Sed0020701 vs. NCBI nr
Match: XP_022940185.1 (autophagy-related protein 18f-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1322.8 bits (3422), Expect = 0.0e+00
Identity = 702/881 (79.68%), Postives = 759/881 (86.15%), Query Frame = 0

Query: 6   VKRQGTIARSGRAHGFISSSFRTFSSYVKIVSSGASTVARSAASVASSFVDKDDESNGSQ 65
           VK+QGT+ARSGRAH FISSSFR FSSY+KIVS+GASTVARSAASVASS VDKDDE+NG Q
Sbjct: 7   VKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDDEANGFQ 66

Query: 66  VNWAGFDKLEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPI 125
           VNWAGFDKLEWD++V+R VLLLG+ SGFQVWDVEEANNVQDLVSRYDGSVSY+QVLPRPI
Sbjct: 67  VNWAGFDKLEWDDNVVRRVLLLGYCSGFQVWDVEEANNVQDLVSRYDGSVSYMQVLPRPI 126

Query: 126 PSMRSGDKFAESRPLLVLSAYGSIAAGFNIQEQLATSGNAAITKGQELADDNLMPTFVRF 185
           PSMRSGDKF ESRPLLVLSAYGSI AGF IQ+++ATSGNAA+ K QE AD   MPTFVRF
Sbjct: 127 PSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRIATSGNAAVPKVQEPADAGFMPTFVRF 186

Query: 186 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVSGFP 245
           YSLKSQTYVHELKFRSVVYSVRCSPLVVAISL TQIHCINATTLETEHIIHTNP+ SGF 
Sbjct: 187 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLVTQIHCINATTLETEHIIHTNPVASGFL 246

Query: 246 GSGEGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKP-ASLSHSSSNGSVVAHYAKE 305
            SG GIGYGPLA+G RWLAYSGS I ASN GRV PQHLKP ASLSHSS NGS+VAHYAKE
Sbjct: 247 CSGGGIGYGPLALGSRWLAYSGSPITASNVGRVTPQHLKPSASLSHSSLNGSLVAHYAKE 306

Query: 306 SSKQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSVGM 365
           SSKQLAAGLVT+GDKGIKKLSRYYS+LLP+SNNS Q G+QGL GIG L  H ADADSVGM
Sbjct: 307 SSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVADADSVGM 366

Query: 366 VIVKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSPAS 425
           VIVKDIISK VITQFRAHKSPISALCFDPSGTILVTAS+ GHNINVF I PSS  NS  S
Sbjct: 367 VIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSNSSVS 426

Query: 426 GTGASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVNFPSAD 485
            TGASY H+Y LQRGFTNAVIQDISFSY+SNWIMISSSRGTSHLFAINP+GGQVNFPS  
Sbjct: 427 STGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQVNFPS-- 486

Query: 486 TSSITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHGSNGWRGTVT 545
           TSS TR  G VV AR+T+R +DSGLHMP KQNECA  GPITLSAVSRIHHGSNGWRGTV+
Sbjct: 487 TSSATRTDGLVVPARQTVRLVDSGLHMPRKQNECATRGPITLSAVSRIHHGSNGWRGTVS 546

Query: 546 SAAAAATGRMGSTSGAIASAFHDCKGNALHMSHGSSDARYHILVFSPSGSMIQYVLRVDS 605
           SAAAAATGR+GS +GAIASAFH+CKGNALHM HGSS+ARYHILVF+P+GSMIQYVLRV  
Sbjct: 547 SAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQYVLRVGL 606

Query: 606 DSTIVLPKSSTAHELEPGLDGRLVVEAIQKWNIFQKQNRRAQDNSSDIYGDNGALDFHHN 665
           DS I LP SSTA +L P  D R+ VEA+QKWNI QKQNRRA+DN+ DIYGDNGALD + N
Sbjct: 607 DSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGALDCNKN 666

Query: 666 YSGEMNGKPIFHEASGTVLKAKTCREEQHHLYISEAELQMHGARSPLWMKPEIYFQVMTT 725
           Y  EMNGKP   EASGTV KAKTC EEQHHLYISEAELQMH  R+PLW KPEIYFQVM  
Sbjct: 667 YCVEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIYFQVMAR 726

Query: 726 FAIKGDEIDHPGEIEIERIPTRMVEARSKGLIPVFDCLQTPKISQTWSLNRNKD----HQ 785
             +KGDEID  GE EIERIPTRMVEARSK L+PVF+ LQ PKISQT SLNRN D    HQ
Sbjct: 727 DGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQLPKISQTRSLNRNSDQLLLHQ 786

Query: 786 KSGLFENGRQSR-NDSSPPGSVINNEPDRNGSHVHKMVTEETKGIVNDYEGSRS-QTQHN 845
           KS LFENGRQSR ND+SP GSVI+N P   G H +K+VT ETKG+VN YEG RS   + +
Sbjct: 787 KSDLFENGRQSRGNDASPNGSVISNGPKGTGKHGYKLVT-ETKGVVNGYEGPRSLHPELD 846

Query: 846 NVNNCTESLSMESTTQAKSVNNHENGMEVENHFEDMGDVFD 880
           NVNNCTESLSME   Q KSVNNHE+G+EVE+ FE + D+FD
Sbjct: 847 NVNNCTESLSME-CCQPKSVNNHESGVEVEDDFEGICDLFD 883

BLAST of Sed0020701 vs. NCBI nr
Match: KAG6607820.1 (Autophagy-related protein 18f, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1322.4 bits (3421), Expect = 0.0e+00
Identity = 703/883 (79.61%), Postives = 763/883 (86.41%), Query Frame = 0

Query: 6   VKRQGTIARSGRAHGFISSSFRTFSSYVKIVSSGASTVARSAASVASSFVDKDDESNGSQ 65
           VK+QGT+ARSGRAH FISSSFR FSSY+KIVS+GASTVARSAASVASS VDK+DE+NG Q
Sbjct: 7   VKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKEDEANGFQ 66

Query: 66  VNWAGFDKLEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPI 125
           VNWAGFDKLEWD++V+R VLLLG+RSGFQVWDVEEANNVQDLVSRYDGSVSY+QVLPRPI
Sbjct: 67  VNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQVLPRPI 126

Query: 126 PSMRSGDKFAESRPLLVLSAYGSIAAGFNIQEQLATSGNAAITKGQELADDNLMPTFVRF 185
           PSMRSGDKF ESRPLLVLSAYGSI AGF IQ++LATSGNAA+ K QELA    MPTFVRF
Sbjct: 127 PSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFMPTFVRF 186

Query: 186 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVSGFP 245
           YSLKSQTYVHELKFRSVVYSVRCSPLVVAISL TQIHCINATTLETEHIIHTNP+ SGF 
Sbjct: 187 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLFTQIHCINATTLETEHIIHTNPVASGFL 246

Query: 246 GSGEGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKP-ASLSHSSSNGSVVAHYAKE 305
           GSG GIGYGPLA+G RWLAYSGS I+ASN GRV PQHLKP ASLSHSS NGS+VAHYAKE
Sbjct: 247 GSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLVAHYAKE 306

Query: 306 SSKQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSVGM 365
           SSKQLAAGLVT+GDKGIKKLSRYYS+LLP+SNNS Q G+QGL GIG L  H ADADSVGM
Sbjct: 307 SSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVADADSVGM 366

Query: 366 VIVKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMP--SSGLNSP 425
           VIVKDIISK VITQFRAHKSPISALCFDPSGTILVTAS+ GHNINVF I P  SS  NS 
Sbjct: 367 VIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSSSNSS 426

Query: 426 ASGTGASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVNFPS 485
            S TGASY H+Y LQRGFTNAVIQDISFSY+SNWIMISSSRGTSHLFAIN +GGQVNFPS
Sbjct: 427 VSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINSAGGQVNFPS 486

Query: 486 ADTSSITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHGSNGWRGT 545
             TSS TR  G VV AR+T+R +DSGLH+P KQNECA  GPITLSAVSRIHHGSNGWRGT
Sbjct: 487 --TSSATRTDGLVVPARQTVRLVDSGLHIPCKQNECATGGPITLSAVSRIHHGSNGWRGT 546

Query: 546 VTSAAAAATGRMGSTSGAIASAFHDCKGNALHMSHGSSDARYHILVFSPSGSMIQYVLRV 605
           V+SAAAAATGR+GS +GAIASAFH+CKGNALHM HGSS+ARYHILVF+P+GSMIQYVLRV
Sbjct: 547 VSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQYVLRV 606

Query: 606 DSDSTIVLPKSSTAHELEPGLDGRLVVEAIQKWNIFQKQNRRAQDNSSDIYGDNGALDFH 665
             DS + LP SSTA +L P  D R+ VEA+QKWNI QKQNRRA+DN+ DIYGDNGALD +
Sbjct: 607 GLDSAVALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGALDCN 666

Query: 666 HNYSGEMNGKPIFHEASGTVLKAKTCREEQHHLYISEAELQMHGARSPLWMKPEIYFQVM 725
            NY GEMNGKP   EASGTV KAKTC EEQHHLYISEAELQMH  R+PLW KPEIYFQVM
Sbjct: 667 KNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIYFQVM 726

Query: 726 TTFAIKGDEIDHPGEIEIERIPTRMVEARSKGLIPVFDCLQTPKISQTWSLNRNKD---- 785
               +KGDEID  GE EIERIPTRMVEARSK L+PVF+ LQ+PKISQT SLNRN D    
Sbjct: 727 ARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSDQLLL 786

Query: 786 HQKSGLFENGRQS-RNDSSPPGSVINNEPDRNGSHVHKMVTEETKGIVNDYEGSRS-QTQ 845
           HQKS LFENGRQS RND+SP GSVI+N P   G H +KMVT ETKG+VN YEG RS   +
Sbjct: 787 HQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVT-ETKGVVNGYEGPRSLHPE 846

Query: 846 HNNVNNCTESLSMESTTQAKSVNNHENGMEVENHFEDMGDVFD 880
            +NVNNCTESLSME   Q KSVNNHE+G+EVE+ FE + D+FD
Sbjct: 847 LDNVNNCTESLSME-CCQPKSVNNHESGVEVEDDFEGICDLFD 885

BLAST of Sed0020701 vs. NCBI nr
Match: KAG7015218.1 (Autophagy-related protein 18f, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1319.7 bits (3414), Expect = 0.0e+00
Identity = 703/883 (79.61%), Postives = 762/883 (86.30%), Query Frame = 0

Query: 6   VKRQGTIARSGRAHGFISSSFRTFSSYVKIVSSGASTVARSAASVASSFVDKDDESNGSQ 65
           VK+QGT+ARSGRAH FISSSFR FSSY+KIVS+GASTVARSAASVASS VDK+DE+NG Q
Sbjct: 7   VKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKEDEANGFQ 66

Query: 66  VNWAGFDKLEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPI 125
           VNWAGFDKLEWD++V+R VLLLG+RSGFQVWDVEEANNVQDLVSRYDGSVSY+QVLPRPI
Sbjct: 67  VNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQVLPRPI 126

Query: 126 PSMRSGDKFAESRPLLVLSAYGSIAAGFNIQEQLATSGNAAITKGQELADDNLMPTFVRF 185
           PSMRSGDKF ESRPLLVLSAYGSI AGF IQ++LATSGNAA+ K QELA    MPTFVRF
Sbjct: 127 PSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFMPTFVRF 186

Query: 186 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVSGFP 245
           YSLKSQTYVHELKFRSVVYSVRCS LVVAISL TQIHCINATTLETEHIIHTNP+ SGF 
Sbjct: 187 YSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNPVASGFL 246

Query: 246 GSGEGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKP-ASLSHSSSNGSVVAHYAKE 305
           GSG GIGYGPLA+G RWLAYSGS I+ASN GRV PQHLKP ASLSHSS NGS+VAHYAKE
Sbjct: 247 GSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLVAHYAKE 306

Query: 306 SSKQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSVGM 365
           SSKQLAAGLVT+GDKGIKKLSRYYS+LLP+SNNS Q G+QGL GIG L  H ADADSVGM
Sbjct: 307 SSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVADADSVGM 366

Query: 366 VIVKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMP--SSGLNSP 425
           VIVKDIISK VITQFRAHKSPISALCFDPSGTILVTAS+ GHNINVF I P  SS  NS 
Sbjct: 367 VIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSSSNSS 426

Query: 426 ASGTGASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVNFPS 485
            S TGASY H+Y LQRGFTNAVIQDISFSY+SNWIMISSSRGTSHLFAINP+GGQVNFPS
Sbjct: 427 VSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQVNFPS 486

Query: 486 ADTSSITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHGSNGWRGT 545
             TSS TR  G VV AR+T+  +DSGLHMP KQNECA  GPITLSAVSRIHHGSNGWRGT
Sbjct: 487 --TSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNGWRGT 546

Query: 546 VTSAAAAATGRMGSTSGAIASAFHDCKGNALHMSHGSSDARYHILVFSPSGSMIQYVLRV 605
           V+SAAAAATGR+GS +GAIASAFH+CKGNALHM HGSS+ARYHILVF+P+GSMIQYVL+V
Sbjct: 547 VSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQYVLQV 606

Query: 606 DSDSTIVLPKSSTAHELEPGLDGRLVVEAIQKWNIFQKQNRRAQDNSSDIYGDNGALDFH 665
             DS I LP SSTA +L P  D R+ VEA+QKWNI QKQNRRA+DN+ DIYGDNGALD +
Sbjct: 607 GLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGALDCN 666

Query: 666 HNYSGEMNGKPIFHEASGTVLKAKTCREEQHHLYISEAELQMHGARSPLWMKPEIYFQVM 725
            NY GEMNGKP   EASGTV KAKTC EEQHHLYISEAELQMH  R+PLW KPEIYFQVM
Sbjct: 667 KNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIYFQVM 726

Query: 726 TTFAIKGDEIDHPGEIEIERIPTRMVEARSKGLIPVFDCLQTPKISQTWSLNRNKD---- 785
               +KGDEID  GE EIERIPTRMVEARSK L+PVF+ LQ+PKISQT SLNRN D    
Sbjct: 727 ARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSDQLLL 786

Query: 786 HQKSGLFENGRQS-RNDSSPPGSVINNEPDRNGSHVHKMVTEETKGIVNDYEGSRS-QTQ 845
           HQKS LFENGRQS RND+SP GSVI+N P   G H +KMVT ETKG+VN YEG RS   +
Sbjct: 787 HQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVT-ETKGVVNGYEGPRSLHPE 846

Query: 846 HNNVNNCTESLSMESTTQAKSVNNHENGMEVENHFEDMGDVFD 880
            +NVNNCTESLSME   Q KSVNNHE+G+EVE+ FE + D+FD
Sbjct: 847 LDNVNNCTESLSME-CCQPKSVNNHESGVEVEDDFEGICDLFD 885

BLAST of Sed0020701 vs. NCBI nr
Match: XP_023524854.1 (autophagy-related protein 18f-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1315.4 bits (3403), Expect = 0.0e+00
Identity = 699/881 (79.34%), Postives = 757/881 (85.93%), Query Frame = 0

Query: 6   VKRQGTIARSGRAHGFISSSFRTFSSYVKIVSSGASTVARSAASVASSFVDKDDESNGSQ 65
           VK+QGT+ RSGRAH FISSSFR FSSY+KIVS+GASTVARSAASVASS VDKDDE+NG Q
Sbjct: 7   VKQQGTVPRSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDDEANGFQ 66

Query: 66  VNWAGFDKLEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPI 125
           VNWAGFDKLEWD++V+R VLLLG+RSGFQVWDVEEANNVQDLVSRYDGSVSY+QVLPRPI
Sbjct: 67  VNWAGFDKLEWDDNVVRSVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQVLPRPI 126

Query: 126 PSMRSGDKFAESRPLLVLSAYGSIAAGFNIQEQLATSGNAAITKGQELADDNLMPTFVRF 185
           PSMRSGDKF ESRPLLVLSAYGSI AGF IQ+++ATSGNAA+ K QELAD   MPTFVRF
Sbjct: 127 PSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRIATSGNAAVPKVQELADAGFMPTFVRF 186

Query: 186 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVSGFP 245
           YSLKSQTYVHELKFRSVVYSVRCSPLVVAISL TQIHCINATTLETEHIIHTNP+ SGF 
Sbjct: 187 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLVTQIHCINATTLETEHIIHTNPVASGFL 246

Query: 246 GSGEGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKP-ASLSHSSSNGSVVAHYAKE 305
           GSG GIGYGPLA+GPRWLAYSGS I ASN GRV PQHLKP ASLSHSS NGS+VAHYAKE
Sbjct: 247 GSGGGIGYGPLALGPRWLAYSGSPITASNVGRVTPQHLKPSASLSHSSLNGSLVAHYAKE 306

Query: 306 SSKQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSVGM 365
           SSKQLAAGLVT+GDKGIKKLSRYYS+LLP+SNNS Q G+ GL GIG L  H ADADSVGM
Sbjct: 307 SSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQPGAHGLNGIGYLKDHVADADSVGM 366

Query: 366 VIVKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSPAS 425
           VIVKDIISK VITQFRAHKSPISALCFDPSGTILVTAS+ GHNINVF I PSS  NSP S
Sbjct: 367 VIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSNSPVS 426

Query: 426 GTGASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVNFPSAD 485
            TGASY H+Y LQRGFTNAVIQDISFSY+SNWIMISSSRGTSHLFAINP+GGQV  PS  
Sbjct: 427 STGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQV--PS-- 486

Query: 486 TSSITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHGSNGWRGTVT 545
           TSS  R  G VV AR+T+R +DSGLHMP KQNECA  GP+TLSAVSRIHHGSNGWRGTV+
Sbjct: 487 TSSAIRTDGLVVPARQTVRLVDSGLHMPRKQNECATGGPMTLSAVSRIHHGSNGWRGTVS 546

Query: 546 SAAAAATGRMGSTSGAIASAFHDCKGNALHMSHGSSDARYHILVFSPSGSMIQYVLRVDS 605
           SAAAAATGR+GS +GAIASAFH+CKGNA  + HGSS+ARYHILVF+P+GSMIQYVLRV  
Sbjct: 547 SAAAAATGRIGSVTGAIASAFHNCKGNAFPVDHGSSEARYHILVFTPTGSMIQYVLRVGL 606

Query: 606 DSTIVLPKSSTAHELEPGLDGRLVVEAIQKWNIFQKQNRRAQDNSSDIYGDNGALDFHHN 665
           DS I LP SSTA +L P  D R+ VEA+QKWNI QKQNRRA+DN+ DIYGDNGALD + N
Sbjct: 607 DSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGALDCNKN 666

Query: 666 YSGEMNGKPIFHEASGTVLKAKTCREEQHHLYISEAELQMHGARSPLWMKPEIYFQVMTT 725
           Y GEMNGKPI  EASGTV KAKTC EEQHHLYISEAELQMH  R+PLW KPEIYFQVM  
Sbjct: 667 YCGEMNGKPIVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIYFQVMAR 726

Query: 726 FAIKGDEIDHPGEIEIERIPTRMVEARSKGLIPVFDCLQTPKISQTWSLNRNKD----HQ 785
             +KGDE D  GE EIERIPTRMVEARSK L+PVF+ LQ+P ISQT SLNRN D    HQ
Sbjct: 727 DGVKGDETDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPIISQTRSLNRNSDQLLLHQ 786

Query: 786 KSGLFENGRQS-RNDSSPPGSVINNEPDRNGSHVHKMVTEETKGIVNDYEGSR-SQTQHN 845
           KS LFENGRQS RND SP GSVI+N P   G H +KMVT ETKG+VN YEG R S  + +
Sbjct: 787 KSDLFENGRQSRRNDVSPDGSVISNGPKGTGKHGYKMVT-ETKGVVNGYEGPRSSHPELD 846

Query: 846 NVNNCTESLSMESTTQAKSVNNHENGMEVENHFEDMGDVFD 880
           NVNNCTESLSME   Q KSVNNHE+G+EVE+ FE + D+FD
Sbjct: 847 NVNNCTESLSME-CCQPKSVNNHESGVEVEDDFEGICDLFD 881

BLAST of Sed0020701 vs. ExPASy Swiss-Prot
Match: Q9FH32 (Autophagy-related protein 18f OS=Arabidopsis thaliana OX=3702 GN=ATG18F PE=2 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 1.7e-141
Identity = 341/775 (44.00%), Postives = 451/775 (58.19%), Query Frame = 0

Query: 10  GTIARSGRAHGFISSSFRTFSSYVKIVSSGASTVARSAASVASSFVDKDDESNGSQVNWA 69
           G ++RS R      SSFR  S+ +K++SSGASTVARSA S ASS V    ES+  QV WA
Sbjct: 15  GVVSRSAR------SSFRALSNCLKVISSGASTVARSAVSAASSAV----ESHHDQVLWA 74

Query: 70  GFDKLEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPIPSMR 129
           GFD L+ ++   R VLLL F+SGFQVWDVE+  NV  +VS +DG   ++Q+L  PI S  
Sbjct: 75  GFDNLQKEDGDTRRVLLLAFKSGFQVWDVEDTENVHVIVSAHDGQAFFMQMLLNPINSGV 134

Query: 130 SGDKFAESRPLL-VLSAYGS--IAAGFNIQEQLATSGNAAITKGQELADDNLMPTFVRFY 189
             D+F +SRPLL V   Y S  I++     E +AT                  PT V  Y
Sbjct: 135 LDDRFYKSRPLLAVCGDYSSKKISSDNPGSETVAT------------------PTNVYVY 194

Query: 190 SLKSQTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVSGFPG 249
           SLKSQ+YVH LKFR+ +YSVRC   +VA+  A QI C +A TLE ++ I TN IV G   
Sbjct: 195 SLKSQSYVHTLKFRATIYSVRCCSRIVAVLQAAQIDCFDAATLEMDYRIVTNSIVCG--- 254

Query: 250 SGEGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKPASLSHSSSNGSVVAHYAKESS 309
              G+GYGPLAVGPRW+AYSGS I A+++  +    +   SLS SS +   VA +A++SS
Sbjct: 255 -SLGVGYGPLAVGPRWIAYSGSRI-ATSSSAIFTSEI--VSLSTSSPS---VAQFARDSS 314

Query: 310 KQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSVGMVI 369
           KQLA+G+  LGDKG + L++Y S++LP           GLKGIG  N   ADA+S+GMVI
Sbjct: 315 KQLASGIANLGDKGYRSLTKYCSEVLPNPY------IPGLKGIGVGNEKVADAESIGMVI 374

Query: 370 VKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSPASGT 429
           VKDI +K VITQF+AHKSPISALCFD SG +LVTAS+ GHNINVF+IMP+   +     T
Sbjct: 375 VKDITNKSVITQFKAHKSPISALCFDQSGLLLVTASIQGHNINVFRIMPTISTSRAVKTT 434

Query: 430 GASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVNFPSADTS 489
             ++ H++ LQRGFTNAVIQDI FS +SN I++ SSRGTSHLF INP             
Sbjct: 435 --TFAHLFRLQRGFTNAVIQDICFSKDSNLIVVGSSRGTSHLFEINP------------- 494

Query: 490 SITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHG-SNGWRGTVTS 549
                                         E   + P+ +SA+SRI  G S+GW GTV+ 
Sbjct: 495 ------------------------------EKEGDAPVPMSAISRIRSGNSSGWIGTVSG 554

Query: 550 AAAAATGRM-GSTSGAIASAFHDCKGNALHMSHGS---SDARYHILVFSPSGSMIQYVLR 609
           AA+AA G + GS  G + S F  C   + +  +GS     ++ ++LVF+PSG M QY LR
Sbjct: 555 AASAAAGMVAGSVPGTVTSTFCYCDEKSNNNYYGSVADMCSKTNLLVFAPSGCMTQYALR 614

Query: 610 VDSDSTIVLPKSSTAHELEPGL--DGRLVVEAIQKWNIFQKQNRR-AQDNSSDIYGDNGA 669
                      + T  + E GL  +G+L V+ I++W++ Q Q+RR   D  SDIYG   +
Sbjct: 615 EHQVGVGHETAAMTGFDSESGLETEGKLAVDPIRRWSMIQNQSRRETHDPHSDIYGGGTS 674

Query: 670 LDFHHNYSGEMNGKPIFHEASGTVLKAKTCREEQHHLYISEAELQMH--GARSPLWMKPE 729
           +D       E+  K    EA     K  T   ++ HLYI EAE Q      + PLW + +
Sbjct: 675 VDSKSKVFPEVVRKQSVEEAWKVSKKGTTRVVDKRHLYIYEAEQQQTHLPTQLPLWARRK 698

Query: 730 IYFQVMTTFAIKGDEIDHPG--EIEIERIPTRMVEARSKGLIPVFDCLQTPKISQ 770
             FQ +     +G+EI   G  E+EIE I TR +EAR++ L+PV+  LQ+P+  Q
Sbjct: 735 FRFQELV--LNRGEEISGGGGREMEIEGIQTRTIEARTRDLVPVWGYLQSPRSQQ 698

BLAST of Sed0020701 vs. ExPASy Swiss-Prot
Match: Q8GUL1 (Autophagy-related protein 18g OS=Arabidopsis thaliana OX=3702 GN=ATG18G PE=2 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 2.3e-125
Identity = 318/836 (38.04%), Postives = 468/836 (55.98%), Query Frame = 0

Query: 16  GRAHGFISSSFRTFSSYVKIVSSGASTVARS----AASVASSFVDKDDESNGSQVNWAGF 75
           G+  G + +SF+  SS +K VS+ A+ VA S     ASVA+S    +D+ +  QV WAGF
Sbjct: 7   GKNSGLLPNSFKIISSCLKTVSANATNVASSVRSAGASVAASISAAEDDKD--QVTWAGF 66

Query: 76  DKLEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPIPSMRSG 135
             LE  + V R VLLLG+++GFQV+DVE+A+N  +LVS+  G VS++Q+ P P    RSG
Sbjct: 67  GILELGQHVTRHVLLLGYQNGFQVFDVEDASNFNELVSKRGGPVSFLQMQPLP---ARSG 126

Query: 136 DK--FAESRPLLVLSAYGSIAAGFNIQEQLATSGNAAI--TKGQELADDNLMPTFVRFYS 195
           D   F  S PLL++ A G    G  +    + +G+ A   +   +  D    PT VRFYS
Sbjct: 127 DHEGFWNSHPLLLVVA-GDETNGTGLGHSFSQNGSLARDGSSDSKAGDAINYPTTVRFYS 186

Query: 196 LKSQTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVSGF--P 255
           L+S +YV+ L+FRS V  +RCS  VVA+ LA QI+C++A TLE +  + T P+       
Sbjct: 187 LRSHSYVYVLRFRSSVCMIRCSSRVVAVGLANQIYCVDALTLENKFSVLTYPVPQPVRQG 246

Query: 256 GSGEGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKPA-SLSHSSSNG--SVVAHYA 315
            +   +GYGP+AVGPRWLAY+    M   TGR+ PQ    + SLS SSS+G  S +A YA
Sbjct: 247 TTRVNVGYGPMAVGPRWLAYASKSSMTMKTGRLSPQTFTSSPSLSPSSSSGGSSFMARYA 306

Query: 316 KESSKQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSV 375
            ESSKQLA GL+ LGD G K LS+Y  D+LP+ + S  S +   K +G ++G  +DA++ 
Sbjct: 307 MESSKQLANGLINLGDMGYKTLSKYCQDMLPDGSTSPASPNAIWK-VGGVSG--SDAENA 366

Query: 376 GMVIVKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSP 435
           GMV VKD++S  +++QF+AH SPISALCFDPSGT+LVTASV G+NINVF+IMPS   N+P
Sbjct: 367 GMVAVKDLVSGALVSQFKAHTSPISALCFDPSGTLLVTASVCGNNINVFQIMPSRSHNAP 426

Query: 436 ASGT---GASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVN 495
              +    +S+ H++ L RG T+A++QDI FS +S W+ I SS+GT H+F +N SG    
Sbjct: 427 GDLSYEWESSHVHLFKLHRGITSAIVQDICFSQQSQWVAIISSKGTCHIFVLNSSGSDAA 486

Query: 496 FPSADTSSITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHGSNGW 555
           F   +    TR   S +    T         + S Q   +    + LS VSRI + S GW
Sbjct: 487 FQPCEGEEPTRLPASSLPWWFT-------QSLSSNQQSLSPPTAVALSVVSRIKYSSFGW 546

Query: 556 RGTVTSAAAAATGRMGSTSGAIASAFHDCKGNALHMSHGSSDARYHILVFSPSGSMIQY- 615
             TV++A  AATG++   SGA+A+ FH    + L + +  ++A  HILV++PSG ++Q+ 
Sbjct: 547 LNTVSNATTAATGKVFVPSGAVAAVFHKSVTHDLQL-NSRTNALEHILVYTPSGHVVQHE 606

Query: 616 ----VLRVDSDSTIVLPKSSTAHELEPGLDGRLVVEAIQKWNIFQKQN---------RRA 675
               V     ++ + + K+S     E  L  R+ VE IQ W++ ++ +         +  
Sbjct: 607 LLPSVCTESPENGLRVQKTSHVQVQEDDL--RVKVEPIQWWDVCRRSDWLETEERLPKSI 666

Query: 676 QDNSSDIYGDNGALDFHHNYSGEMNGKPIFHEASGTVLKAKTCRE---EQHHLYISEAEL 735
            +   D+   +  L  H +    ++    F E        K+C E   E+ H Y+S  E+
Sbjct: 667 TEKQYDLETVSNHLTSHEDACLSLDMNSHFSEDK----YLKSCSEKPPERSHCYLSNFEV 726

Query: 736 QMHGARSPLWMKPEIYFQVMTTFAIKGDEIDHPGEIEIERIPTRMVEARSKGLIPVFDCL 795
           ++     P+W   +I F VM +     D     GE EIE++P   +E + K L+PVFD  
Sbjct: 727 KVTSGMLPVWQNSKISFHVMDS---PRDSSSTGGEFEIEKVPAHELEIKQKKLLPVFDHF 786

Query: 796 QTPKIS-------QTWSLNRNKDHQKSGLFENGRQSRNDSSPPGSVINNEPDRNGS 812
            + K +       + +  +    HQ +G  +  +   N  S PGS+ + E    GS
Sbjct: 787 HSTKATLEDRFSMKCYHTSATGSHQVNG--KICQDIINCHSKPGSIESAESSEEGS 814

BLAST of Sed0020701 vs. ExPASy Swiss-Prot
Match: Q8H1Q5 (Autophagy-related protein 18h OS=Arabidopsis thaliana OX=3702 GN=ATG18H PE=2 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 9.0e-114
Identity = 296/809 (36.59%), Postives = 444/809 (54.88%), Query Frame = 0

Query: 14  RSGRAHGFISSSFRTFSSYVKIVSSGASTVARSAASVASSFVDKDDESNGSQVNWAGFDK 73
           ++   +GF+ +S +  S+ ++  SSG   V  ++ASVA+S +  D      QV W+ FD+
Sbjct: 20  KNNGTNGFLPNSLKFISTCIRTASSG---VRSASASVAAS-LSSDSHELKDQVLWSSFDR 79

Query: 74  LEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPIPSMRSG-D 133
           L   E   + VLLLG+ +GFQV D++++N+V + VSR D  V+++Q+  +P+P+   G +
Sbjct: 80  LHTSESSFKNVLLLGYTNGFQVLDIDDSNDVTEFVSRRDDPVTFLQM--QPLPAKCDGVE 139

Query: 134 KFAESRPLLVLSAYGSIAAGFNIQEQLATSGNAAITKGQE--LADDNLMPTFVRFYSLKS 193
            F  S P+L+  A  +  +G      + TS + ++  G E  LA   L PT VRFYSL+S
Sbjct: 140 GFRSSHPILLAVADEAKGSG-----PIVTSRDGSVRNGYEDPLA---LSPTVVRFYSLRS 199

Query: 194 QTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVS-GFPG-SG 253
             YVH L+FRS VY VRCSP +VA+ L +QI+C +A TLE +  + + P+   G  G SG
Sbjct: 200 HNYVHVLRFRSTVYMVRCSPRIVAVGLGSQIYCFDALTLENKFSVLSYPVPQLGNQGISG 259

Query: 254 EGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKPASLSHSSS--NGSVVAHYAKESS 313
             +GYGP+AVG RWLAY+ +  ++S+ GR+ PQ++ P  +S S+S  NG++VA YA ESS
Sbjct: 260 VNVGYGPMAVGARWLAYASNSPLSSSIGRLSPQNVTPPGVSPSTSPNNGNLVARYAMESS 319

Query: 314 KQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSVGMVI 373
           K LAAGL+ LGDKG K +S+Y  DL  +      S S G K +G +  H+A++D VG VI
Sbjct: 320 KHLAAGLLNLGDKGYKTISKYCQDLKHDGPGPSLSSSPGRK-VGRVGSHSAESDVVGTVI 379

Query: 374 VKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSPASGT 433
           VKD  S+ +I QFRAH SPISALCFDPSGT+LVTAS+ G+NINVF+IMP+   N P + +
Sbjct: 380 VKDFESRAIIAQFRAHTSPISALCFDPSGTLLVTASIHGNNINVFRIMPTPTKNGPGAQS 439

Query: 434 ---GASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVNFPSA 493
               +S+  +Y L RG T+AVIQDI FS  S WI I SS+ T H++ ++P GG+ N    
Sbjct: 440 YDWSSSHVPLYKLHRGMTSAVIQDICFSSYSQWIAIVSSKSTCHIYVLSPFGGE-NVLEI 499

Query: 494 DTSSITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHGSNGWRGTV 553
             S      G  ++   ++    S   M +          +TLS VSRI   +       
Sbjct: 500 RNSQF---DGPTLAPTLSLPWWSSPSFM-TTHFSYPPPASVTLSVVSRIKCNN------F 559

Query: 554 TSAAAAATGRMGSTSGAIASAFHDCKGNALHMSHGSSDARYHILVFSPSGSMIQYVL--R 613
             AA++  G+    SG +A+ FH    +    S  SS A  ++LV++PSG ++QY L   
Sbjct: 560 FHAASSVVGKPTFPSGCLAAVFHQ---SVPQESQSSSPALDYLLVYTPSGHVVQYKLIPS 619

Query: 614 VDSDSTIVLPKSSTAHELEPGLDGRLVVEAIQKWNIFQKQNRRAQDNSSDIYGDNGALDF 673
           +  D      ++     L    + R+ VE +Q W++ ++ +    +   +I G       
Sbjct: 620 LGGDQAESNTRNGATSGLTSEEELRVKVEPVQCWDVCRRAD--WPEREENICG------- 679

Query: 674 HHNYSGEMNGKPIFHEASGTVLKAKTCREEQHHLYISEAELQMHGARSPLWMKPEIYFQV 733
              Y G  N +     +     + +T   E+HH+Y++ AE+ ++  R P+W   EI F  
Sbjct: 680 -LTYGGRKNAELTVDTSDS---EDQTKPLEKHHVYLANAEVLINSGRKPIWQNSEISFYP 739

Query: 734 MTTFAIKGDEIDH---PGEIEIERIPTRMVEARSKGLIPVFDCLQTPKISQTWSLNRNKD 793
           M      G  ++     GE EI ++    V+ R K L+PV+D          ++  RN+ 
Sbjct: 740 MYPPDSDGKNLNSHQGGGETEIGKVSANEVDIRRKDLLPVYD-----NFHSVYTSMRNRG 775

Query: 794 HQKSGLFENGRQS-RNDSSPPGSVINNEP 807
                 F   R S  + SS PG V    P
Sbjct: 800 ------FSGERDSDSSSSSDPGQVKEMHP 775

BLAST of Sed0020701 vs. ExPASy Swiss-Prot
Match: Q9H6U6 (BCAS3 microtubule associated cell migration factor OS=Homo sapiens OX=9606 GN=BCAS3 PE=1 SV=3)

HSP 1 Score: 152.9 bits (385), Expect = 1.7e-35
Identity = 114/407 (28.01%), Postives = 198/407 (48.65%), Query Frame = 0

Query: 84  VLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPIPSMRSGDKFAESRPLLVL 143
           ++++G+  G QVW +  +   Q+L S   G +   ++LP P    +  D FAE RPLL  
Sbjct: 92  LIMIGYSDGMQVWSIPISGEAQELFSVRHGPIRAARILPAPQFGAQKCDNFAEKRPLL-- 151

Query: 144 SAYGSIAAGFNIQEQLATSGNAAITKGQELADDNLMPTF--VRFYSLKSQTYVHELKFRS 203
                      + + + +SG +              P +  V  YSL++   V  ++F++
Sbjct: 152 ----------GVCKSIGSSGTS--------------PPYCCVDLYSLRTGEMVKSIQFKT 211

Query: 204 VVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVSGFPGSGEGIGYGPLAVGPR 263
            +Y + C+  ++ + L  +I   ++ T   +       + S +P  G  +   P+A+G R
Sbjct: 212 PIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFF-----VTSCYPCPGPNM--NPIALGSR 271

Query: 264 WLAYSGSMIMASNTGRVIPQHLKPASLSHSSSNGSVVAHYAK---ESSKQLAAGLVTLGD 323
           WLAY+ + ++  +  R               + G  +  Y      ++K L +GL  +G 
Sbjct: 272 WLAYAENKLIRCHQSR-------------GGACGDNIQSYTATVISAAKTLKSGLTMVG- 331

Query: 324 KGIKKL-----SRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSVG---MVIVKDI 383
           K + +L     S    D +   +NSR+S    + GI T+     D ++VG   +++ +D 
Sbjct: 332 KVVTQLTGTLPSGVTEDDVAIHSNSRRSPL--VPGIITV----IDTETVGEGQVLVSEDS 391

Query: 384 ISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSPASGTGASY 443
            S  ++  F AH+ P+  + F+ SG +LVT   LGH+ +VF+I     L  P S +  + 
Sbjct: 392 DSDGIVAHFPAHEKPVCCMAFNTSGMLLVTTDTLGHDFHVFQI-----LTHPWSSSQCAV 440

Query: 444 GHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQ 478
            H+Y L RG T A +QDI FS++  W+++S+ RGTSH+F INP GGQ
Sbjct: 452 HHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQ 440

BLAST of Sed0020701 vs. ExPASy Swiss-Prot
Match: Q8CCN5 (BCAS3 microtubule associated cell migration factor OS=Mus musculus OX=10090 GN=Bcas3 PE=1 SV=2)

HSP 1 Score: 152.1 bits (383), Expect = 2.8e-35
Identity = 114/407 (28.01%), Postives = 197/407 (48.40%), Query Frame = 0

Query: 84  VLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPIPSMRSGDKFAESRPLLVL 143
           ++++G+  G QVW +  +   Q+L S   G V   ++LP P    +  D FAE RPLL  
Sbjct: 92  LVMIGYSDGMQVWGIPISGEAQELFSVRHGPVRAARILPAPQLGAQKCDNFAEKRPLL-- 151

Query: 144 SAYGSIAAGFNIQEQLATSGNAAITKGQELADDNLMPTF--VRFYSLKSQTYVHELKFRS 203
                      + + + +SG                P +  V  YSL++   V  ++F++
Sbjct: 152 ----------GVCKSIGSSGTT--------------PPYCCVDLYSLRTGEMVKSIQFKT 211

Query: 204 VVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVSGFPGSGEGIGYGPLAVGPR 263
            +Y + C+  ++ + L  +I   ++ T   +       + S +P  G  +   P+A+G R
Sbjct: 212 PIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFF-----VTSCYPCPGPNM--NPIALGSR 271

Query: 264 WLAYSGSMIMASNTGRVIPQHLKPASLSHSSSNGSVVAHYAK---ESSKQLAAGLVTLGD 323
           WLAY+ + ++  +  R               + G  +  Y      ++K L +GL  +G 
Sbjct: 272 WLAYAENKLIRCHQSR-------------GGACGDNIQSYTATVLSAAKTLKSGLTMVG- 331

Query: 324 KGIKKLSRYYSDLLPESN-----NSRQSGSQGLKGIGTLNGHAADADSVG---MVIVKDI 383
           K + +L+      + E +     NSR+S    + GI T+     D ++VG   +++ +D 
Sbjct: 332 KVVTQLTGTLPSGVTEDDVALHCNSRRSPL--VPGIITV----IDTETVGEGQVLVSEDS 391

Query: 384 ISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSPASGTGASY 443
            S  ++  F AH+ P+  + F+ SG +LVT   LGH+ +VF+I     L  P S +  + 
Sbjct: 392 DSDGIVAHFPAHEKPVCCMAFNTSGMLLVTTDTLGHDFHVFQI-----LTHPWSSSQCAV 440

Query: 444 GHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQ 478
            H+Y L RG T A +QDI FS++  W+++S+ RGTSH+F INP GGQ
Sbjct: 452 HHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQ 440

BLAST of Sed0020701 vs. ExPASy TrEMBL
Match: A0A6J1FPV0 (autophagy-related protein 18f-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445888 PE=4 SV=1)

HSP 1 Score: 1322.8 bits (3422), Expect = 0.0e+00
Identity = 702/881 (79.68%), Postives = 759/881 (86.15%), Query Frame = 0

Query: 6   VKRQGTIARSGRAHGFISSSFRTFSSYVKIVSSGASTVARSAASVASSFVDKDDESNGSQ 65
           VK+QGT+ARSGRAH FISSSFR FSSY+KIVS+GASTVARSAASVASS VDKDDE+NG Q
Sbjct: 7   VKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDDEANGFQ 66

Query: 66  VNWAGFDKLEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPI 125
           VNWAGFDKLEWD++V+R VLLLG+ SGFQVWDVEEANNVQDLVSRYDGSVSY+QVLPRPI
Sbjct: 67  VNWAGFDKLEWDDNVVRRVLLLGYCSGFQVWDVEEANNVQDLVSRYDGSVSYMQVLPRPI 126

Query: 126 PSMRSGDKFAESRPLLVLSAYGSIAAGFNIQEQLATSGNAAITKGQELADDNLMPTFVRF 185
           PSMRSGDKF ESRPLLVLSAYGSI AGF IQ+++ATSGNAA+ K QE AD   MPTFVRF
Sbjct: 127 PSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRIATSGNAAVPKVQEPADAGFMPTFVRF 186

Query: 186 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVSGFP 245
           YSLKSQTYVHELKFRSVVYSVRCSPLVVAISL TQIHCINATTLETEHIIHTNP+ SGF 
Sbjct: 187 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLVTQIHCINATTLETEHIIHTNPVASGFL 246

Query: 246 GSGEGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKP-ASLSHSSSNGSVVAHYAKE 305
            SG GIGYGPLA+G RWLAYSGS I ASN GRV PQHLKP ASLSHSS NGS+VAHYAKE
Sbjct: 247 CSGGGIGYGPLALGSRWLAYSGSPITASNVGRVTPQHLKPSASLSHSSLNGSLVAHYAKE 306

Query: 306 SSKQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSVGM 365
           SSKQLAAGLVT+GDKGIKKLSRYYS+LLP+SNNS Q G+QGL GIG L  H ADADSVGM
Sbjct: 307 SSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVADADSVGM 366

Query: 366 VIVKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSPAS 425
           VIVKDIISK VITQFRAHKSPISALCFDPSGTILVTAS+ GHNINVF I PSS  NS  S
Sbjct: 367 VIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSNSSVS 426

Query: 426 GTGASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVNFPSAD 485
            TGASY H+Y LQRGFTNAVIQDISFSY+SNWIMISSSRGTSHLFAINP+GGQVNFPS  
Sbjct: 427 STGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQVNFPS-- 486

Query: 486 TSSITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHGSNGWRGTVT 545
           TSS TR  G VV AR+T+R +DSGLHMP KQNECA  GPITLSAVSRIHHGSNGWRGTV+
Sbjct: 487 TSSATRTDGLVVPARQTVRLVDSGLHMPRKQNECATRGPITLSAVSRIHHGSNGWRGTVS 546

Query: 546 SAAAAATGRMGSTSGAIASAFHDCKGNALHMSHGSSDARYHILVFSPSGSMIQYVLRVDS 605
           SAAAAATGR+GS +GAIASAFH+CKGNALHM HGSS+ARYHILVF+P+GSMIQYVLRV  
Sbjct: 547 SAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQYVLRVGL 606

Query: 606 DSTIVLPKSSTAHELEPGLDGRLVVEAIQKWNIFQKQNRRAQDNSSDIYGDNGALDFHHN 665
           DS I LP SSTA +L P  D R+ VEA+QKWNI QKQNRRA+DN+ DIYGDNGALD + N
Sbjct: 607 DSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGALDCNKN 666

Query: 666 YSGEMNGKPIFHEASGTVLKAKTCREEQHHLYISEAELQMHGARSPLWMKPEIYFQVMTT 725
           Y  EMNGKP   EASGTV KAKTC EEQHHLYISEAELQMH  R+PLW KPEIYFQVM  
Sbjct: 667 YCVEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIYFQVMAR 726

Query: 726 FAIKGDEIDHPGEIEIERIPTRMVEARSKGLIPVFDCLQTPKISQTWSLNRNKD----HQ 785
             +KGDEID  GE EIERIPTRMVEARSK L+PVF+ LQ PKISQT SLNRN D    HQ
Sbjct: 727 DGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQLPKISQTRSLNRNSDQLLLHQ 786

Query: 786 KSGLFENGRQSR-NDSSPPGSVINNEPDRNGSHVHKMVTEETKGIVNDYEGSRS-QTQHN 845
           KS LFENGRQSR ND+SP GSVI+N P   G H +K+VT ETKG+VN YEG RS   + +
Sbjct: 787 KSDLFENGRQSRGNDASPNGSVISNGPKGTGKHGYKLVT-ETKGVVNGYEGPRSLHPELD 846

Query: 846 NVNNCTESLSMESTTQAKSVNNHENGMEVENHFEDMGDVFD 880
           NVNNCTESLSME   Q KSVNNHE+G+EVE+ FE + D+FD
Sbjct: 847 NVNNCTESLSME-CCQPKSVNNHESGVEVEDDFEGICDLFD 883

BLAST of Sed0020701 vs. ExPASy TrEMBL
Match: A0A6J1J1X9 (autophagy-related protein 18f-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480521 PE=4 SV=1)

HSP 1 Score: 1313.9 bits (3399), Expect = 0.0e+00
Identity = 693/881 (78.66%), Postives = 754/881 (85.58%), Query Frame = 0

Query: 6   VKRQGTIARSGRAHGFISSSFRTFSSYVKIVSSGASTVARSAASVASSFVDKDDESNGSQ 65
           VK+QG +ARSGRAH FISSSFR FSSY+KIVS+GASTVARSAASVASS  DKDDE+NG Q
Sbjct: 7   VKQQGAVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLADKDDEANGFQ 66

Query: 66  VNWAGFDKLEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPI 125
           VNWAGFDKLEWD++V++ VLLLGFRSGFQVWD+EEANNVQDLVS YDGSVSY+QVLPRPI
Sbjct: 67  VNWAGFDKLEWDDNVVQRVLLLGFRSGFQVWDIEEANNVQDLVSCYDGSVSYMQVLPRPI 126

Query: 126 PSMRSGDKFAESRPLLVLSAYGSIAAGFNIQEQLATSGNAAITKGQELADDNLMPTFVRF 185
           P MRSGDKF ESRPLLVLSAYGSI +GF IQ++LATSGNAA+ K QELAD   MPTFVRF
Sbjct: 127 PLMRSGDKFTESRPLLVLSAYGSITSGFKIQDRLATSGNAAVPKVQELADAGFMPTFVRF 186

Query: 186 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVSGFP 245
           YSLKSQTYVHELKFRSVVYSVRCSPLVVAISL TQIHCINATTLETEHIIH NP+ SGFP
Sbjct: 187 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLVTQIHCINATTLETEHIIHANPVASGFP 246

Query: 246 GSGEGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKP-ASLSHSSSNGSVVAHYAKE 305
           GSG GIGYGPLA+G RWLAYSGS I ASN GRV PQHLKP A LSHSS NGS+VAHYAKE
Sbjct: 247 GSGGGIGYGPLALGSRWLAYSGSPITASNVGRVTPQHLKPSAGLSHSSLNGSLVAHYAKE 306

Query: 306 SSKQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSVGM 365
           SSK LAAGLVT+GDKGIKKLSRYYS+LLP+SNNS Q G+ GL GIG L  H ADADSVG+
Sbjct: 307 SSKHLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQPGAHGLNGIGYLKDHVADADSVGV 366

Query: 366 VIVKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSPAS 425
           VIVKDIISK VITQFRAHKSPISALCFDPSGTILVTAS+ GHNINVF IMPSS  NSP S
Sbjct: 367 VIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNIMPSSSSNSPVS 426

Query: 426 GTGASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVNFPSAD 485
            TGASY H+Y LQRGFTNAVIQDISFSY+SNWIMISSSRGTSHLFAINP+GGQVNFPS  
Sbjct: 427 STGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQVNFPS-- 486

Query: 486 TSSITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHGSNGWRGTVT 545
           T S TR  G VV AR+T+R +DSGLHMP KQNECA  GPITLSAVSRIHHGSNGW+GTV+
Sbjct: 487 TGSATRIDGLVVPARQTVRLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNGWQGTVS 546

Query: 546 SAAAAATGRMGSTSGAIASAFHDCKGNALHMSHGSSDARYHILVFSPSGSMIQYVLRVDS 605
           SAAAAATGR+GS +GAIASAFH+CKGNALH+ HGSS+ARYHILVF+P+GSMIQYVLRV  
Sbjct: 547 SAAAAATGRIGSVTGAIASAFHNCKGNALHVDHGSSEARYHILVFTPTGSMIQYVLRVGL 606

Query: 606 DSTIVLPKSSTAHELEPGLDGRLVVEAIQKWNIFQKQNRRAQDNSSDIYGDNGALDFHHN 665
           DS I LP  ST  +L P LD R+ VEA+QKWNI QKQN RA+DN+ DIYGDNGALD   N
Sbjct: 607 DSAIALPIFSTGLDLVPELDARVAVEAVQKWNISQKQNHRARDNNVDIYGDNGALDCKKN 666

Query: 666 YSGEMNGKPIFHEASGTVLKAKTCREEQHHLYISEAELQMHGARSPLWMKPEIYFQVMTT 725
           Y GEMNGKPI  EASGTV KAKTC  EQHHLYISE ELQMH   +PLW KPEIYFQVM  
Sbjct: 667 YCGEMNGKPIVLEASGTVSKAKTCTVEQHHLYISEVELQMHADHTPLWTKPEIYFQVMAK 726

Query: 726 FAIKGDEIDHPGEIEIERIPTRMVEARSKGLIPVFDCLQTPKISQTWSLNRNKD----HQ 785
             +KGDEID+ GE EIERIPTRMVEARSK L+PVF+ LQ+PKISQT SLNRN D    HQ
Sbjct: 727 DGVKGDEIDNLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSDQLLLHQ 786

Query: 786 KSGLFENGRQS-RNDSSPPGSVINNEPDRNGSHVHKMVTEETKGIVNDYEGSR-SQTQHN 845
           KS LFEN RQS RND+SP GSVINN P   G H HKMVT ETKG+VN Y+G R S  + +
Sbjct: 787 KSDLFENERQSRRNDASPDGSVINNGPKGTGKHGHKMVT-ETKGVVNGYKGPRSSHPEPD 846

Query: 846 NVNNCTESLSMESTTQAKSVNNHENGMEVENHFEDMGDVFD 880
           NVNNCTESLSME  +Q KSVNNHE+G+EVE+ FE + D+FD
Sbjct: 847 NVNNCTESLSME-CSQPKSVNNHESGVEVEDDFEGICDLFD 883

BLAST of Sed0020701 vs. ExPASy TrEMBL
Match: A0A6J1FHR8 (autophagy-related protein 18f-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445888 PE=4 SV=1)

HSP 1 Score: 1313.5 bits (3398), Expect = 0.0e+00
Identity = 696/877 (79.36%), Postives = 756/877 (86.20%), Query Frame = 0

Query: 6   VKRQGTIARSGRAHGFISSSFRTFSSYVKIVSSGASTVARSAASVASSFVDKDDESNGSQ 65
           VK+QGT+ARSGRAH FISSSFR FSSY+KIVS+GASTVARSAASVASS VDKDDE+NG Q
Sbjct: 7   VKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDDEANGFQ 66

Query: 66  VNWAGFDKLEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPI 125
           VNWAGFDKLEWD++V+R VLLLG+ SGFQVWDVEEANNVQDLVSRYDGSVSY+QVLPRPI
Sbjct: 67  VNWAGFDKLEWDDNVVRRVLLLGYCSGFQVWDVEEANNVQDLVSRYDGSVSYMQVLPRPI 126

Query: 126 PSMRSGDKFAESRPLLVLSAYGSIAAGFNIQEQLATSGNAAITKGQELADDNLMPTFVRF 185
           PSMRSGDKF ESRPLLVLSAYGSI AGF IQ+++ATSGNAA+ K QE AD   MPTFVRF
Sbjct: 127 PSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRIATSGNAAVPKVQEPADAGFMPTFVRF 186

Query: 186 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVSGFP 245
           YSLKSQTYVHELKFRSVVYSVRCSPLVVAISL TQIHCINATTLETEHIIHTNP+ SGF 
Sbjct: 187 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLVTQIHCINATTLETEHIIHTNPVASGFL 246

Query: 246 GSGEGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKP-ASLSHSSSNGSVVAHYAKE 305
            SG GIGYGPLA+G RWLAYSGS I ASN GRV PQHLKP ASLSHSS NGS+VAHYAKE
Sbjct: 247 CSGGGIGYGPLALGSRWLAYSGSPITASNVGRVTPQHLKPSASLSHSSLNGSLVAHYAKE 306

Query: 306 SSKQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSVGM 365
           SSKQLAAGLVT+GDKGIKKLSRYYS+LLP+SNNS Q G+QGL GIG L  H ADADSVGM
Sbjct: 307 SSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVADADSVGM 366

Query: 366 VIVKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSPAS 425
           VIVKDIISK VITQFRAHKSPISALCFDPSGTILVTAS+ GHNINVF I PSS  NS  S
Sbjct: 367 VIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSNSSVS 426

Query: 426 GTGASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVNFPSAD 485
            TGASY H+Y LQRGFTNAVIQDISFSY+SNWIMISSSRGTSHLFAINP+GGQVNFPS  
Sbjct: 427 STGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQVNFPS-- 486

Query: 486 TSSITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHGSNGWRGTVT 545
           TSS TR  G VV AR+T+R +DSGLHMP KQNECA  GPITLSAVSRIHHGSNGWRGTV+
Sbjct: 487 TSSATRTDGLVVPARQTVRLVDSGLHMPRKQNECATRGPITLSAVSRIHHGSNGWRGTVS 546

Query: 546 SAAAAATGRMGSTSGAIASAFHDCKGNALHMSHGSSDARYHILVFSPSGSMIQYVLRVDS 605
           SAAAAATGR+GS +GAIASAFH+CKGNALHM HGSS+ARYHILVF+P+GSMIQYVLRV  
Sbjct: 547 SAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQYVLRVGL 606

Query: 606 DSTIVLPKSSTAHELEPGLDGRLVVEAIQKWNIFQKQNRRAQDNSSDIYGDNGALDFHHN 665
           DS I LP SSTA +L P  D R+ VEA+QKWNI QKQNRRA+DN+ DIYGDNGALD + N
Sbjct: 607 DSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGALDCNKN 666

Query: 666 YSGEMNGKPIFHEASGTVLKAKTCREEQHHLYISEAELQMHGARSPLWMKPEIYFQVMTT 725
           Y  EMNGKP   EASGTV KAKTC EEQHHLYISEAELQMH  R+PLW KPEIYFQVM  
Sbjct: 667 YCVEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIYFQVMAR 726

Query: 726 FAIKGDEIDHPGEIEIERIPTRMVEARSKGLIPVFDCLQTPKISQTWSLNRNKDHQKSGL 785
             +KGDEID  GE EIERIPTRMVEARSK L+PVF+ LQ PKISQT + ++   HQKS L
Sbjct: 727 DGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQLPKISQTRNSDQLLLHQKSDL 786

Query: 786 FENGRQSR-NDSSPPGSVINNEPDRNGSHVHKMVTEETKGIVNDYEGSRS-QTQHNNVNN 845
           FENGRQSR ND+SP GSVI+N P   G H +K+VT ETKG+VN YEG RS   + +NVNN
Sbjct: 787 FENGRQSRGNDASPNGSVISNGPKGTGKHGYKLVT-ETKGVVNGYEGPRSLHPELDNVNN 846

Query: 846 CTESLSMESTTQAKSVNNHENGMEVENHFEDMGDVFD 880
           CTESLSME   Q KSVNNHE+G+EVE+ FE + D+FD
Sbjct: 847 CTESLSME-CCQPKSVNNHESGVEVEDDFEGICDLFD 879

BLAST of Sed0020701 vs. ExPASy TrEMBL
Match: A0A0A0LD33 (BCAS3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G415110 PE=4 SV=1)

HSP 1 Score: 1309.7 bits (3388), Expect = 0.0e+00
Identity = 688/887 (77.56%), Postives = 764/887 (86.13%), Query Frame = 0

Query: 1   MRNGG-VKRQGTIARSGRAHGFISSSFRTFSSYVKIVSSGASTVARSAASVASSFVDKDD 60
           MRN G +K+QGT+ARSGRAH F+S+SFR FSSY+KIVS+GASTVARSAASVASS VDKDD
Sbjct: 27  MRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDD 86

Query: 61  ESNGSQVNWAGFDKLEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQ 120
           E+N SQVNWAGFDKLEWD++V+R VLLLG RSGFQVWDVEEANNVQ+LV RYDGSVSY+Q
Sbjct: 87  EANASQVNWAGFDKLEWDDNVIRQVLLLGIRSGFQVWDVEEANNVQELVCRYDGSVSYMQ 146

Query: 121 VLPRPIPSMRSGDKFAESRPLLVLSAYGSIAAGFNIQEQLATSGNAAITKGQELADDNLM 180
           VLPR IPSMRSGDKFAESRPL+VLSAYGSIAA FNIQ++LA+SGNA I K QE+ D N M
Sbjct: 147 VLPRLIPSMRSGDKFAESRPLIVLSAYGSIAANFNIQDRLASSGNATIPKSQEVVDGNFM 206

Query: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNP 240
           PTFVRFYSLKSQTYVHELKFRS VYSV+CSPLVVAISLATQIHCINATTLE EHII TNP
Sbjct: 207 PTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCINATTLEKEHIILTNP 266

Query: 241 IVSGFPGSGEGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKP-ASLSHSSSNGSVV 300
           +VSGFPGSG G+GYGPLA+GPRWLAYSGS I+ SNTGRV+PQHLKP AS SHSSSNGS+V
Sbjct: 267 VVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLSNTGRVVPQHLKPSASFSHSSSNGSLV 326

Query: 301 AHYAKESSKQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAAD 360
           AHYAKESSK LAAG+VTLGDKGIKKLSRYYS+LLPESNNS QSG+QGLKGIGTLNGH AD
Sbjct: 327 AHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKGIGTLNGHMAD 386

Query: 361 ADSVGMVIVKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSG 420
           ADS+GM IVKDIISK VITQF+AHKSPISALCFDPSGTILVTASV GH+INVFKIMPSS 
Sbjct: 387 ADSIGMAIVKDIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSC 446

Query: 421 LNSPASGTGASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQV 480
             S  S T ASY H+Y LQRGFTNAVIQDISFSY+SNWIMISSSRGTSHLFAINPSGGQV
Sbjct: 447 SKSSISSTAASYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPSGGQV 506

Query: 481 NFPSADTSSITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHGSNG 540
           NFPSAD   I RNGG VV AR+T+RR+DSGLHMPSKQN+C    P+TLSAV+RIHHGSNG
Sbjct: 507 NFPSADI--IARNGGPVVPARQTVRRVDSGLHMPSKQNQCTTGSPLTLSAVTRIHHGSNG 566

Query: 541 WRGTVTSAAAAATGRMGSTSGAIASAFHDCKGNALHMSHGSSDARYHILVFSPSGSMIQY 600
           WRGTV+SAAAAATG+MG  SGAIASAFH+CKGN +H+ +GSS+ RYHILVFSPSGSMIQY
Sbjct: 567 WRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVHVDNGSSEVRYHILVFSPSGSMIQY 626

Query: 601 VLRVDSDSTIVLPKSSTAHELEPGLDGRLVVEAIQKWNIFQKQNRRAQDNSSDIYGDNGA 660
            LRV  DST+VLP+SSTA EL   LD RLVVEAIQKWNI QKQNRR+QDNS DIYGDNG 
Sbjct: 627 ALRVGLDSTVVLPRSSTALELVSELDSRLVVEAIQKWNISQKQNRRSQDNSIDIYGDNGG 686

Query: 661 LDFHHNYSGEMNGKPIFHEASGTVLKAKTCREEQHHLYISEAELQMHGARSPLWMKPEIY 720
            + + NY  EMNG P+  EA G + K K CREEQ+HLYISEAELQMH AR+PLW KPEIY
Sbjct: 687 FNCNKNYCEEMNGNPVL-EAGGNIFKTKACREEQYHLYISEAELQMHAARTPLWTKPEIY 746

Query: 721 FQVMTTFAIKGDEIDHPGEIEIERIPTRMVEARSKGLIPVFDCLQTPKISQTWSLNRNKD 780
           FQVM    +K DEIDHPGE++IERIPTRM+EARSK L+PVFD LQ+ KISQ   LN N D
Sbjct: 747 FQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDLVPVFDYLQSSKISQPRYLNSNSD 806

Query: 781 ----HQKSGLFENGRQS-RNDSSPPGSVINNEPDRNGSHVHKMVTEETKGIVNDYEGSRS 840
                QKSG FENGRQS R+ ++P  SV+ N  +  G H +K VT ETKGIVN+ E  RS
Sbjct: 807 QLLLQQKSGQFENGRQSWRSAANPHDSVVGNGREVTGRHGYKRVT-ETKGIVNEPECPRS 866

Query: 841 QTQHNNVNNCTESLSMESTTQAKSVNN-HENGMEVENHFEDMGDVFD 880
           QTQ +NVN+CTES+SMES  Q KSVNN H++G+ VENHFED+GD F+
Sbjct: 867 QTQFSNVNSCTESISMES--QPKSVNNHHKSGLRVENHFEDLGDEFE 907

BLAST of Sed0020701 vs. ExPASy TrEMBL
Match: A0A6J1J1Q2 (autophagy-related protein 18f-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111480521 PE=4 SV=1)

HSP 1 Score: 1304.7 bits (3375), Expect = 0.0e+00
Identity = 687/877 (78.34%), Postives = 751/877 (85.63%), Query Frame = 0

Query: 6   VKRQGTIARSGRAHGFISSSFRTFSSYVKIVSSGASTVARSAASVASSFVDKDDESNGSQ 65
           VK+QG +ARSGRAH FISSSFR FSSY+KIVS+GASTVARSAASVASS  DKDDE+NG Q
Sbjct: 7   VKQQGAVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLADKDDEANGFQ 66

Query: 66  VNWAGFDKLEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPI 125
           VNWAGFDKLEWD++V++ VLLLGFRSGFQVWD+EEANNVQDLVS YDGSVSY+QVLPRPI
Sbjct: 67  VNWAGFDKLEWDDNVVQRVLLLGFRSGFQVWDIEEANNVQDLVSCYDGSVSYMQVLPRPI 126

Query: 126 PSMRSGDKFAESRPLLVLSAYGSIAAGFNIQEQLATSGNAAITKGQELADDNLMPTFVRF 185
           P MRSGDKF ESRPLLVLSAYGSI +GF IQ++LATSGNAA+ K QELAD   MPTFVRF
Sbjct: 127 PLMRSGDKFTESRPLLVLSAYGSITSGFKIQDRLATSGNAAVPKVQELADAGFMPTFVRF 186

Query: 186 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVSGFP 245
           YSLKSQTYVHELKFRSVVYSVRCSPLVVAISL TQIHCINATTLETEHIIH NP+ SGFP
Sbjct: 187 YSLKSQTYVHELKFRSVVYSVRCSPLVVAISLVTQIHCINATTLETEHIIHANPVASGFP 246

Query: 246 GSGEGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKP-ASLSHSSSNGSVVAHYAKE 305
           GSG GIGYGPLA+G RWLAYSGS I ASN GRV PQHLKP A LSHSS NGS+VAHYAKE
Sbjct: 247 GSGGGIGYGPLALGSRWLAYSGSPITASNVGRVTPQHLKPSAGLSHSSLNGSLVAHYAKE 306

Query: 306 SSKQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSVGM 365
           SSK LAAGLVT+GDKGIKKLSRYYS+LLP+SNNS Q G+ GL GIG L  H ADADSVG+
Sbjct: 307 SSKHLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQPGAHGLNGIGYLKDHVADADSVGV 366

Query: 366 VIVKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSPAS 425
           VIVKDIISK VITQFRAHKSPISALCFDPSGTILVTAS+ GHNINVF IMPSS  NSP S
Sbjct: 367 VIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNIMPSSSSNSPVS 426

Query: 426 GTGASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVNFPSAD 485
            TGASY H+Y LQRGFTNAVIQDISFSY+SNWIMISSSRGTSHLFAINP+GGQVNFPS  
Sbjct: 427 STGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQVNFPS-- 486

Query: 486 TSSITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHGSNGWRGTVT 545
           T S TR  G VV AR+T+R +DSGLHMP KQNECA  GPITLSAVSRIHHGSNGW+GTV+
Sbjct: 487 TGSATRIDGLVVPARQTVRLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNGWQGTVS 546

Query: 546 SAAAAATGRMGSTSGAIASAFHDCKGNALHMSHGSSDARYHILVFSPSGSMIQYVLRVDS 605
           SAAAAATGR+GS +GAIASAFH+CKGNALH+ HGSS+ARYHILVF+P+GSMIQYVLRV  
Sbjct: 547 SAAAAATGRIGSVTGAIASAFHNCKGNALHVDHGSSEARYHILVFTPTGSMIQYVLRVGL 606

Query: 606 DSTIVLPKSSTAHELEPGLDGRLVVEAIQKWNIFQKQNRRAQDNSSDIYGDNGALDFHHN 665
           DS I LP  ST  +L P LD R+ VEA+QKWNI QKQN RA+DN+ DIYGDNGALD   N
Sbjct: 607 DSAIALPIFSTGLDLVPELDARVAVEAVQKWNISQKQNHRARDNNVDIYGDNGALDCKKN 666

Query: 666 YSGEMNGKPIFHEASGTVLKAKTCREEQHHLYISEAELQMHGARSPLWMKPEIYFQVMTT 725
           Y GEMNGKPI  EASGTV KAKTC  EQHHLYISE ELQMH   +PLW KPEIYFQVM  
Sbjct: 667 YCGEMNGKPIVLEASGTVSKAKTCTVEQHHLYISEVELQMHADHTPLWTKPEIYFQVMAK 726

Query: 726 FAIKGDEIDHPGEIEIERIPTRMVEARSKGLIPVFDCLQTPKISQTWSLNRNKDHQKSGL 785
             +KGDEID+ GE EIERIPTRMVEARSK L+PVF+ LQ+PKISQT + ++   HQKS L
Sbjct: 727 DGVKGDEIDNLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRNSDQLLLHQKSDL 786

Query: 786 FENGRQS-RNDSSPPGSVINNEPDRNGSHVHKMVTEETKGIVNDYEGSR-SQTQHNNVNN 845
           FEN RQS RND+SP GSVINN P   G H HKMVT ETKG+VN Y+G R S  + +NVNN
Sbjct: 787 FENERQSRRNDASPDGSVINNGPKGTGKHGHKMVT-ETKGVVNGYKGPRSSHPEPDNVNN 846

Query: 846 CTESLSMESTTQAKSVNNHENGMEVENHFEDMGDVFD 880
           CTESLSME  +Q KSVNNHE+G+EVE+ FE + D+FD
Sbjct: 847 CTESLSME-CSQPKSVNNHESGVEVEDDFEGICDLFD 879

BLAST of Sed0020701 vs. TAIR 10
Match: AT5G54730.1 (homolog of yeast autophagy 18 (ATG18) F )

HSP 1 Score: 505.0 bits (1299), Expect = 1.2e-142
Identity = 341/775 (44.00%), Postives = 451/775 (58.19%), Query Frame = 0

Query: 10  GTIARSGRAHGFISSSFRTFSSYVKIVSSGASTVARSAASVASSFVDKDDESNGSQVNWA 69
           G ++RS R      SSFR  S+ +K++SSGASTVARSA S ASS V    ES+  QV WA
Sbjct: 15  GVVSRSAR------SSFRALSNCLKVISSGASTVARSAVSAASSAV----ESHHDQVLWA 74

Query: 70  GFDKLEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPIPSMR 129
           GFD L+ ++   R VLLL F+SGFQVWDVE+  NV  +VS +DG   ++Q+L  PI S  
Sbjct: 75  GFDNLQKEDGDTRRVLLLAFKSGFQVWDVEDTENVHVIVSAHDGQAFFMQMLLNPINSGV 134

Query: 130 SGDKFAESRPLL-VLSAYGS--IAAGFNIQEQLATSGNAAITKGQELADDNLMPTFVRFY 189
             D+F +SRPLL V   Y S  I++     E +AT                  PT V  Y
Sbjct: 135 LDDRFYKSRPLLAVCGDYSSKKISSDNPGSETVAT------------------PTNVYVY 194

Query: 190 SLKSQTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVSGFPG 249
           SLKSQ+YVH LKFR+ +YSVRC   +VA+  A QI C +A TLE ++ I TN IV G   
Sbjct: 195 SLKSQSYVHTLKFRATIYSVRCCSRIVAVLQAAQIDCFDAATLEMDYRIVTNSIVCG--- 254

Query: 250 SGEGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKPASLSHSSSNGSVVAHYAKESS 309
              G+GYGPLAVGPRW+AYSGS I A+++  +    +   SLS SS +   VA +A++SS
Sbjct: 255 -SLGVGYGPLAVGPRWIAYSGSRI-ATSSSAIFTSEI--VSLSTSSPS---VAQFARDSS 314

Query: 310 KQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSVGMVI 369
           KQLA+G+  LGDKG + L++Y S++LP           GLKGIG  N   ADA+S+GMVI
Sbjct: 315 KQLASGIANLGDKGYRSLTKYCSEVLPNPY------IPGLKGIGVGNEKVADAESIGMVI 374

Query: 370 VKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSPASGT 429
           VKDI +K VITQF+AHKSPISALCFD SG +LVTAS+ GHNINVF+IMP+   +     T
Sbjct: 375 VKDITNKSVITQFKAHKSPISALCFDQSGLLLVTASIQGHNINVFRIMPTISTSRAVKTT 434

Query: 430 GASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVNFPSADTS 489
             ++ H++ LQRGFTNAVIQDI FS +SN I++ SSRGTSHLF INP             
Sbjct: 435 --TFAHLFRLQRGFTNAVIQDICFSKDSNLIVVGSSRGTSHLFEINP------------- 494

Query: 490 SITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHG-SNGWRGTVTS 549
                                         E   + P+ +SA+SRI  G S+GW GTV+ 
Sbjct: 495 ------------------------------EKEGDAPVPMSAISRIRSGNSSGWIGTVSG 554

Query: 550 AAAAATGRM-GSTSGAIASAFHDCKGNALHMSHGS---SDARYHILVFSPSGSMIQYVLR 609
           AA+AA G + GS  G + S F  C   + +  +GS     ++ ++LVF+PSG M QY LR
Sbjct: 555 AASAAAGMVAGSVPGTVTSTFCYCDEKSNNNYYGSVADMCSKTNLLVFAPSGCMTQYALR 614

Query: 610 VDSDSTIVLPKSSTAHELEPGL--DGRLVVEAIQKWNIFQKQNRR-AQDNSSDIYGDNGA 669
                      + T  + E GL  +G+L V+ I++W++ Q Q+RR   D  SDIYG   +
Sbjct: 615 EHQVGVGHETAAMTGFDSESGLETEGKLAVDPIRRWSMIQNQSRRETHDPHSDIYGGGTS 674

Query: 670 LDFHHNYSGEMNGKPIFHEASGTVLKAKTCREEQHHLYISEAELQMH--GARSPLWMKPE 729
           +D       E+  K    EA     K  T   ++ HLYI EAE Q      + PLW + +
Sbjct: 675 VDSKSKVFPEVVRKQSVEEAWKVSKKGTTRVVDKRHLYIYEAEQQQTHLPTQLPLWARRK 698

Query: 730 IYFQVMTTFAIKGDEIDHPG--EIEIERIPTRMVEARSKGLIPVFDCLQTPKISQ 770
             FQ +     +G+EI   G  E+EIE I TR +EAR++ L+PV+  LQ+P+  Q
Sbjct: 735 FRFQELV--LNRGEEISGGGGREMEIEGIQTRTIEARTRDLVPVWGYLQSPRSQQ 698

BLAST of Sed0020701 vs. TAIR 10
Match: AT1G03380.1 (homolog of yeast autophagy 18 (ATG18) G )

HSP 1 Score: 451.4 bits (1160), Expect = 1.6e-126
Identity = 318/836 (38.04%), Postives = 468/836 (55.98%), Query Frame = 0

Query: 16  GRAHGFISSSFRTFSSYVKIVSSGASTVARS----AASVASSFVDKDDESNGSQVNWAGF 75
           G+  G + +SF+  SS +K VS+ A+ VA S     ASVA+S    +D+ +  QV WAGF
Sbjct: 7   GKNSGLLPNSFKIISSCLKTVSANATNVASSVRSAGASVAASISAAEDDKD--QVTWAGF 66

Query: 76  DKLEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPIPSMRSG 135
             LE  + V R VLLLG+++GFQV+DVE+A+N  +LVS+  G VS++Q+ P P    RSG
Sbjct: 67  GILELGQHVTRHVLLLGYQNGFQVFDVEDASNFNELVSKRGGPVSFLQMQPLP---ARSG 126

Query: 136 DK--FAESRPLLVLSAYGSIAAGFNIQEQLATSGNAAI--TKGQELADDNLMPTFVRFYS 195
           D   F  S PLL++ A G    G  +    + +G+ A   +   +  D    PT VRFYS
Sbjct: 127 DHEGFWNSHPLLLVVA-GDETNGTGLGHSFSQNGSLARDGSSDSKAGDAINYPTTVRFYS 186

Query: 196 LKSQTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVSGF--P 255
           L+S +YV+ L+FRS V  +RCS  VVA+ LA QI+C++A TLE +  + T P+       
Sbjct: 187 LRSHSYVYVLRFRSSVCMIRCSSRVVAVGLANQIYCVDALTLENKFSVLTYPVPQPVRQG 246

Query: 256 GSGEGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKPA-SLSHSSSNG--SVVAHYA 315
            +   +GYGP+AVGPRWLAY+    M   TGR+ PQ    + SLS SSS+G  S +A YA
Sbjct: 247 TTRVNVGYGPMAVGPRWLAYASKSSMTMKTGRLSPQTFTSSPSLSPSSSSGGSSFMARYA 306

Query: 316 KESSKQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSV 375
            ESSKQLA GL+ LGD G K LS+Y  D+LP+ + S  S +   K +G ++G  +DA++ 
Sbjct: 307 MESSKQLANGLINLGDMGYKTLSKYCQDMLPDGSTSPASPNAIWK-VGGVSG--SDAENA 366

Query: 376 GMVIVKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSP 435
           GMV VKD++S  +++QF+AH SPISALCFDPSGT+LVTASV G+NINVF+IMPS   N+P
Sbjct: 367 GMVAVKDLVSGALVSQFKAHTSPISALCFDPSGTLLVTASVCGNNINVFQIMPSRSHNAP 426

Query: 436 ASGT---GASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVN 495
              +    +S+ H++ L RG T+A++QDI FS +S W+ I SS+GT H+F +N SG    
Sbjct: 427 GDLSYEWESSHVHLFKLHRGITSAIVQDICFSQQSQWVAIISSKGTCHIFVLNSSGSDAA 486

Query: 496 FPSADTSSITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHGSNGW 555
           F   +    TR   S +    T         + S Q   +    + LS VSRI + S GW
Sbjct: 487 FQPCEGEEPTRLPASSLPWWFT-------QSLSSNQQSLSPPTAVALSVVSRIKYSSFGW 546

Query: 556 RGTVTSAAAAATGRMGSTSGAIASAFHDCKGNALHMSHGSSDARYHILVFSPSGSMIQY- 615
             TV++A  AATG++   SGA+A+ FH    + L + +  ++A  HILV++PSG ++Q+ 
Sbjct: 547 LNTVSNATTAATGKVFVPSGAVAAVFHKSVTHDLQL-NSRTNALEHILVYTPSGHVVQHE 606

Query: 616 ----VLRVDSDSTIVLPKSSTAHELEPGLDGRLVVEAIQKWNIFQKQN---------RRA 675
               V     ++ + + K+S     E  L  R+ VE IQ W++ ++ +         +  
Sbjct: 607 LLPSVCTESPENGLRVQKTSHVQVQEDDL--RVKVEPIQWWDVCRRSDWLETEERLPKSI 666

Query: 676 QDNSSDIYGDNGALDFHHNYSGEMNGKPIFHEASGTVLKAKTCRE---EQHHLYISEAEL 735
            +   D+   +  L  H +    ++    F E        K+C E   E+ H Y+S  E+
Sbjct: 667 TEKQYDLETVSNHLTSHEDACLSLDMNSHFSEDK----YLKSCSEKPPERSHCYLSNFEV 726

Query: 736 QMHGARSPLWMKPEIYFQVMTTFAIKGDEIDHPGEIEIERIPTRMVEARSKGLIPVFDCL 795
           ++     P+W   +I F VM +     D     GE EIE++P   +E + K L+PVFD  
Sbjct: 727 KVTSGMLPVWQNSKISFHVMDS---PRDSSSTGGEFEIEKVPAHELEIKQKKLLPVFDHF 786

Query: 796 QTPKIS-------QTWSLNRNKDHQKSGLFENGRQSRNDSSPPGSVINNEPDRNGS 812
            + K +       + +  +    HQ +G  +  +   N  S PGS+ + E    GS
Sbjct: 787 HSTKATLEDRFSMKCYHTSATGSHQVNG--KICQDIINCHSKPGSIESAESSEEGS 814

BLAST of Sed0020701 vs. TAIR 10
Match: AT1G54710.1 (homolog of yeast autophagy 18 (ATG18) H )

HSP 1 Score: 412.9 bits (1060), Expect = 6.4e-115
Identity = 296/809 (36.59%), Postives = 444/809 (54.88%), Query Frame = 0

Query: 14  RSGRAHGFISSSFRTFSSYVKIVSSGASTVARSAASVASSFVDKDDESNGSQVNWAGFDK 73
           ++   +GF+ +S +  S+ ++  SSG   V  ++ASVA+S +  D      QV W+ FD+
Sbjct: 20  KNNGTNGFLPNSLKFISTCIRTASSG---VRSASASVAAS-LSSDSHELKDQVLWSSFDR 79

Query: 74  LEWDEDVLRWVLLLGFRSGFQVWDVEEANNVQDLVSRYDGSVSYIQVLPRPIPSMRSG-D 133
           L   E   + VLLLG+ +GFQV D++++N+V + VSR D  V+++Q+  +P+P+   G +
Sbjct: 80  LHTSESSFKNVLLLGYTNGFQVLDIDDSNDVTEFVSRRDDPVTFLQM--QPLPAKCDGVE 139

Query: 134 KFAESRPLLVLSAYGSIAAGFNIQEQLATSGNAAITKGQE--LADDNLMPTFVRFYSLKS 193
            F  S P+L+  A  +  +G      + TS + ++  G E  LA   L PT VRFYSL+S
Sbjct: 140 GFRSSHPILLAVADEAKGSG-----PIVTSRDGSVRNGYEDPLA---LSPTVVRFYSLRS 199

Query: 194 QTYVHELKFRSVVYSVRCSPLVVAISLATQIHCINATTLETEHIIHTNPIVS-GFPG-SG 253
             YVH L+FRS VY VRCSP +VA+ L +QI+C +A TLE +  + + P+   G  G SG
Sbjct: 200 HNYVHVLRFRSTVYMVRCSPRIVAVGLGSQIYCFDALTLENKFSVLSYPVPQLGNQGISG 259

Query: 254 EGIGYGPLAVGPRWLAYSGSMIMASNTGRVIPQHLKPASLSHSSS--NGSVVAHYAKESS 313
             +GYGP+AVG RWLAY+ +  ++S+ GR+ PQ++ P  +S S+S  NG++VA YA ESS
Sbjct: 260 VNVGYGPMAVGARWLAYASNSPLSSSIGRLSPQNVTPPGVSPSTSPNNGNLVARYAMESS 319

Query: 314 KQLAAGLVTLGDKGIKKLSRYYSDLLPESNNSRQSGSQGLKGIGTLNGHAADADSVGMVI 373
           K LAAGL+ LGDKG K +S+Y  DL  +      S S G K +G +  H+A++D VG VI
Sbjct: 320 KHLAAGLLNLGDKGYKTISKYCQDLKHDGPGPSLSSSPGRK-VGRVGSHSAESDVVGTVI 379

Query: 374 VKDIISKVVITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSPASGT 433
           VKD  S+ +I QFRAH SPISALCFDPSGT+LVTAS+ G+NINVF+IMP+   N P + +
Sbjct: 380 VKDFESRAIIAQFRAHTSPISALCFDPSGTLLVTASIHGNNINVFRIMPTPTKNGPGAQS 439

Query: 434 ---GASYGHMYILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVNFPSA 493
               +S+  +Y L RG T+AVIQDI FS  S WI I SS+ T H++ ++P GG+ N    
Sbjct: 440 YDWSSSHVPLYKLHRGMTSAVIQDICFSSYSQWIAIVSSKSTCHIYVLSPFGGE-NVLEI 499

Query: 494 DTSSITRNGGSVVSARRTIRRLDSGLHMPSKQNECAMEGPITLSAVSRIHHGSNGWRGTV 553
             S      G  ++   ++    S   M +          +TLS VSRI   +       
Sbjct: 500 RNSQF---DGPTLAPTLSLPWWSSPSFM-TTHFSYPPPASVTLSVVSRIKCNN------F 559

Query: 554 TSAAAAATGRMGSTSGAIASAFHDCKGNALHMSHGSSDARYHILVFSPSGSMIQYVL--R 613
             AA++  G+    SG +A+ FH    +    S  SS A  ++LV++PSG ++QY L   
Sbjct: 560 FHAASSVVGKPTFPSGCLAAVFHQ---SVPQESQSSSPALDYLLVYTPSGHVVQYKLIPS 619

Query: 614 VDSDSTIVLPKSSTAHELEPGLDGRLVVEAIQKWNIFQKQNRRAQDNSSDIYGDNGALDF 673
           +  D      ++     L    + R+ VE +Q W++ ++ +    +   +I G       
Sbjct: 620 LGGDQAESNTRNGATSGLTSEEELRVKVEPVQCWDVCRRAD--WPEREENICG------- 679

Query: 674 HHNYSGEMNGKPIFHEASGTVLKAKTCREEQHHLYISEAELQMHGARSPLWMKPEIYFQV 733
              Y G  N +     +     + +T   E+HH+Y++ AE+ ++  R P+W   EI F  
Sbjct: 680 -LTYGGRKNAELTVDTSDS---EDQTKPLEKHHVYLANAEVLINSGRKPIWQNSEISFYP 739

Query: 734 MTTFAIKGDEIDH---PGEIEIERIPTRMVEARSKGLIPVFDCLQTPKISQTWSLNRNKD 793
           M      G  ++     GE EI ++    V+ R K L+PV+D          ++  RN+ 
Sbjct: 740 MYPPDSDGKNLNSHQGGGETEIGKVSANEVDIRRKDLLPVYD-----NFHSVYTSMRNRG 775

Query: 794 HQKSGLFENGRQS-RNDSSPPGSVINNEP 807
                 F   R S  + SS PG V    P
Sbjct: 800 ------FSGERDSDSSSSSDPGQVKEMHP 775

BLAST of Sed0020701 vs. TAIR 10
Match: AT5G05150.1 (autophagy-related gene 18E )

HSP 1 Score: 49.3 bits (116), Expect = 1.8e-05
Identity = 33/116 (28.45%), Postives = 52/116 (44.83%), Query Frame = 0

Query: 375 VITQFRAHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSPASGTGASYGHMY 434
           VI   +AH S I+ +     G++L TAS  G  I +F  +  + L               
Sbjct: 196 VIKFIKAHDSAIACMTLTLDGSLLATASTKGTLIRIFNAVDGTLLQE------------- 255

Query: 435 ILQRGFTNAVIQDISFSYESNWIMISSSRGTSHLFAINPSGGQVNFPSADTSSITR 491
             +RG   A I +++ S    W+  SS +GT H+F + P    ++F  A +SS  R
Sbjct: 256 -FRRGVERAEIYNVAISSNLKWVAASSEKGTLHVFRLRPD--ILSFDPASSSSFIR 295

BLAST of Sed0020701 vs. TAIR 10
Match: AT2G40810.1 (homolog of yeast autophagy 18C )

HSP 1 Score: 45.4 bits (106), Expect = 2.7e-04
Identity = 27/91 (29.67%), Postives = 41/91 (45.05%), Query Frame = 0

Query: 381 AHKSPISALCFDPSGTILVTASVLGHNINVFKIMPSSGLNSPASGTGASYGHMYILQRGF 440
           AH S I+ +     G +L TAS  G  I +F  M  + L                ++RG 
Sbjct: 199 AHDSSIACMTLTLDGLLLATASTKGTLIRIFNTMDGTRLQE--------------VRRGV 258

Query: 441 TNAVIQDISFSYESNWIMISSSRGTSHLFAI 472
             A I  I+ S    W+ +SS +GT H+F++
Sbjct: 259 DRADIYSIALSPNVQWLAVSSDKGTVHIFSL 275