Sed0020662 (gene) Chayote v1

BLAST of Sed0020662 vs. NCBI nr
Match: XP_022926839.1 (MACPF domain-containing protein CAD1 [Cucurbita moschata])

HSP 1 Score: 1068.9 bits (2763), Expect = 1.5e-308
Identity = 527/560 (94.11%), Postives = 538/560 (96.07%), Query Frame = 0

Query: 7   TSNMDALTTTLANSIQALGRGFDVTADIRLLYCKGASGSRLVQLDDGLSHGGDLALSDGV 66
           TS MDALT TL NSIQALGRGFDVTADIRLLYCKG  GSRLVQLDD  +   DL LSDGV
Sbjct: 16  TSEMDALTATLCNSIQALGRGFDVTADIRLLYCKGTPGSRLVQLDDASTR--DLHLSDGV 75

Query: 67  VAFNVPDDVRCSSDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQV 126
           V  NVPD V CSSD+RATENVPVCSFHKMAE+FNQKSN+SG IPLGSFNAMFNFSGSWQV
Sbjct: 76  VVPNVPDHVHCSSDTRATENVPVCSFHKMAEYFNQKSNLSGNIPLGSFNAMFNFSGSWQV 135

Query: 127 DAAATKSLAMIGYFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITS 186
           DAAATKSLAMIGYFIPLFKVELKNS LVLHEEIKRAVPYTWDPVSLASFIENFGTHIITS
Sbjct: 136 DAAATKSLAMIGYFIPLFKVELKNSTLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITS 195

Query: 187 ATIGGRDVVYIRQHQSSPLSESEIENYVREVGEQRFLDSKGQSTVGPLSYKDKDVTVIFR 246
           ATIGGRDVVYIRQHQSSPLSESEIENYV+EVGEQRFLDSK QSTVGPLSYKDKDVTVIFR
Sbjct: 196 ATIGGRDVVYIRQHQSSPLSESEIENYVKEVGEQRFLDSKSQSTVGPLSYKDKDVTVIFR 255

Query: 247 RRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLARAIDLYLEYKPPIE 306
           RRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPG+KHLARAIDLYLEYKPPIE
Sbjct: 256 RRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIE 315

Query: 307 DLQYFLDFQVGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLN 366
           DLQYFLDFQ+GRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLN
Sbjct: 316 DLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLN 375

Query: 367 LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV 426
           LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGA KWHGPEEQDSRWFEPIKWKNFSHV
Sbjct: 376 LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGALKWHGPEEQDSRWFEPIKWKNFSHV 435

Query: 427 STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP 486
           STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCT+RRSVWDHSP
Sbjct: 436 STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTVRRSVWDHSP 495

Query: 487 STPATPLKSDGASSSSKTRTGEDKKEDSSGNAGKLAKIVDLTEMSKGPQDIPGHWLVSGA 546
           ST ATP +SDGASSSSKT+TGEDKKEDSS NAGKLAKIVDLTEMSKGPQDIPGHWLV+GA
Sbjct: 496 STSATPSRSDGASSSSKTKTGEDKKEDSSSNAGKLAKIVDLTEMSKGPQDIPGHWLVTGA 555

Query: 547 KLGVDKGRIVLRIKYSLLNY 567
           KLGVDKGRIVLRIKYSLLNY
Sbjct: 556 KLGVDKGRIVLRIKYSLLNY 573

BLAST of Sed0020662 vs. NCBI nr
Match: XP_023518202.1 (MACPF domain-containing protein CAD1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1064.7 bits (2752), Expect = 2.8e-307
Identity = 524/560 (93.57%), Postives = 537/560 (95.89%), Query Frame = 0

Query: 7   TSNMDALTTTLANSIQALGRGFDVTADIRLLYCKGASGSRLVQLDDGLSHGGDLALSDGV 66
           TS MDALT TL NSIQALGRGFDVTADIRLLYCKG  GSRLVQLDD  +   DL LSDGV
Sbjct: 16  TSEMDALTATLCNSIQALGRGFDVTADIRLLYCKGTPGSRLVQLDDASTR--DLHLSDGV 75

Query: 67  VAFNVPDDVRCSSDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQV 126
           V  NVPD V CSSD+RATENVPVCSFHKMAE+FNQKSN+SG IPLGSFNAMFNFSGSWQV
Sbjct: 76  VVPNVPDHVHCSSDTRATENVPVCSFHKMAEYFNQKSNLSGNIPLGSFNAMFNFSGSWQV 135

Query: 127 DAAATKSLAMIGYFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITS 186
           DAAATKSLAMIGYFIPLFKVELKN  LVLH+EIKRAVPYTWDPVSLASFIEN+GTHIITS
Sbjct: 136 DAAATKSLAMIGYFIPLFKVELKNPTLVLHDEIKRAVPYTWDPVSLASFIENYGTHIITS 195

Query: 187 ATIGGRDVVYIRQHQSSPLSESEIENYVREVGEQRFLDSKGQSTVGPLSYKDKDVTVIFR 246
           ATIGGRDVVYIRQHQSSPLSESEIENYV+EVGEQRFLDSK QSTVGPLSYKDKDVTVIFR
Sbjct: 196 ATIGGRDVVYIRQHQSSPLSESEIENYVKEVGEQRFLDSKSQSTVGPLSYKDKDVTVIFR 255

Query: 247 RRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLARAIDLYLEYKPPIE 306
           RRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPG+KHLARAIDLYLEYKPPIE
Sbjct: 256 RRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIE 315

Query: 307 DLQYFLDFQVGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLN 366
           DLQYFLDFQ+GRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLN
Sbjct: 316 DLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLN 375

Query: 367 LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV 426
           LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGA KWHGPEEQDSRWFEPIKWKNFSHV
Sbjct: 376 LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGALKWHGPEEQDSRWFEPIKWKNFSHV 435

Query: 427 STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP 486
           STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCT+RRSVWDHSP
Sbjct: 436 STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTVRRSVWDHSP 495

Query: 487 STPATPLKSDGASSSSKTRTGEDKKEDSSGNAGKLAKIVDLTEMSKGPQDIPGHWLVSGA 546
           ST ATP +SDGASSSSKT+TGEDKKEDSS NAGKLAKIVDLTEMSKGPQDIPGHWLV+GA
Sbjct: 496 STSATPSRSDGASSSSKTKTGEDKKEDSSSNAGKLAKIVDLTEMSKGPQDIPGHWLVTGA 555

Query: 547 KLGVDKGRIVLRIKYSLLNY 567
           KLGVDKGRIVLRIKYSLLNY
Sbjct: 556 KLGVDKGRIVLRIKYSLLNY 573

BLAST of Sed0020662 vs. NCBI nr
Match: XP_022142258.1 (MACPF domain-containing protein CAD1 [Momordica charantia])

HSP 1 Score: 1063.1 bits (2748), Expect = 8.1e-307
Identity = 519/560 (92.68%), Postives = 541/560 (96.61%), Query Frame = 0

Query: 7   TSNMDALTTTLANSIQALGRGFDVTADIRLLYCKGASGSRLVQLDDGLSHGGDLALSDGV 66
           TS+MDALT TL+NSIQALGRGFDVTADIRLLYCKG  GSRL+QLDD  +H  DL L+DGV
Sbjct: 18  TSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQLDD--AHTRDLVLTDGV 77

Query: 67  VAFNVPDDVRCSSDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQV 126
           +  NVPDD++CSSD+RATENVPVC+FHKMAE+FNQKS ISG IPLGSF+AMFNFSGSWQV
Sbjct: 78  MVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPLGSFDAMFNFSGSWQV 137

Query: 127 DAAATKSLAMIGYFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITS 186
           DAAATKSLAM+GY IPLFKVELKNSNLVL E+IKRAVPYTWDPVSLASFIENFGTHIITS
Sbjct: 138 DAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITS 197

Query: 187 ATIGGRDVVYIRQHQSSPLSESEIENYVREVGEQRFLDSKGQSTVGPLSYKDKDVTVIFR 246
           ATIGGRDVVYIRQHQSSPLSESEIE+YV+EVGEQRFLDSK QSTVGPLSYKDKDVTVIFR
Sbjct: 198 ATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGPLSYKDKDVTVIFR 257

Query: 247 RRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLARAIDLYLEYKPPIE 306
           RRGGDDLVQSHAKWA+TVK+APDVINMTFTPIVSMLEGVPG+KHLARAIDLYLEYKPPIE
Sbjct: 258 RRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIE 317

Query: 307 DLQYFLDFQVGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLN 366
           DLQYFLDFQ+GRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPDQITVGRKPVTGLRLN
Sbjct: 318 DLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLN 377

Query: 367 LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV 426
           LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV
Sbjct: 378 LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV 437

Query: 427 STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP 486
           STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP
Sbjct: 438 STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP 497

Query: 487 STPATPLKSDGASSSSKTRTGEDKKEDSSGNAGKLAKIVDLTEMSKGPQDIPGHWLVSGA 546
           S PATPL+SDGASSSSKTRT EDKKEDSS NAGKLAKIVDLTEMSKGPQDIPGHWLV+GA
Sbjct: 498 SIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMSKGPQDIPGHWLVTGA 557

Query: 547 KLGVDKGRIVLRIKYSLLNY 567
           KLGVDKGRIVLRIKYSLLNY
Sbjct: 558 KLGVDKGRIVLRIKYSLLNY 575

BLAST of Sed0020662 vs. NCBI nr
Match: KAG7026504.1 (MACPF domain-containing protein CAD1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1060.4 bits (2741), Expect = 5.2e-306
Identity = 527/571 (92.29%), Postives = 538/571 (94.22%), Query Frame = 0

Query: 7   TSNMDALTTTLANSIQALGRGFDVTADIRLLYCKGASGSRLVQLDDGLSHGGDLALSDGV 66
           TS MDALT TL NSIQALGRGFDVTADIRLLYCKG  GSRLVQLDD  +   DL LSDGV
Sbjct: 16  TSEMDALTATLCNSIQALGRGFDVTADIRLLYCKGTPGSRLVQLDDASTR--DLHLSDGV 75

Query: 67  VAFNVPDDVRCSSDSRATENVPVCSFHK-----------MAEHFNQKSNISGTIPLGSFN 126
           V  NVPD V CSSD+RATENVPVCSFHK           MAE+FNQKSN+SG IPLGSFN
Sbjct: 76  VVPNVPDHVHCSSDTRATENVPVCSFHKASLELFFQSLLMAEYFNQKSNLSGNIPLGSFN 135

Query: 127 AMFNFSGSWQVDAAATKSLAMIGYFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASF 186
           AMFNFSGSWQVDAAATKSLAMIGYFIPLFKVELKNS LVLHEEIKRAVPYTWDPVSLASF
Sbjct: 136 AMFNFSGSWQVDAAATKSLAMIGYFIPLFKVELKNSTLVLHEEIKRAVPYTWDPVSLASF 195

Query: 187 IENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIENYVREVGEQRFLDSKGQSTVGPLS 246
           IENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIENYV+EVGEQRFLDSK QSTVGPLS
Sbjct: 196 IENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIENYVKEVGEQRFLDSKSQSTVGPLS 255

Query: 247 YKDKDVTVIFRRRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLARAI 306
           YKDKDVTVIFRRRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPG+KHLARAI
Sbjct: 256 YKDKDVTVIFRRRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLARAI 315

Query: 307 DLYLEYKPPIEDLQYFLDFQVGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITV 366
           DLYLEYKPPIEDLQYFLDFQ+GRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITV
Sbjct: 316 DLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITV 375

Query: 367 GRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWF 426
           GRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGA KWHGPEEQDSRWF
Sbjct: 376 GRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGALKWHGPEEQDSRWF 435

Query: 427 EPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGC 486
           EPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGC
Sbjct: 436 EPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGC 495

Query: 487 TIRRSVWDHSPSTPATPLKSDGASSSSKTRTGEDKKEDSSGNAGKLAKIVDLTEMSKGPQ 546
           T+RRSVWDHSPST ATP +SDGASSSSKT+TGEDKKEDSS NAGKLAKIVDLTEMSKGPQ
Sbjct: 496 TVRRSVWDHSPSTSATPSRSDGASSSSKTKTGEDKKEDSSSNAGKLAKIVDLTEMSKGPQ 555

Query: 547 DIPGHWLVSGAKLGVDKGRIVLRIKYSLLNY 567
           DIPGHWLV+GAKLGVDKGRIVLRIKYSLLNY
Sbjct: 556 DIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 584

BLAST of Sed0020662 vs. NCBI nr
Match: XP_023003135.1 (MACPF domain-containing protein CAD1 [Cucurbita maxima])

HSP 1 Score: 1058.9 bits (2737), Expect = 1.5e-305
Identity = 522/559 (93.38%), Postives = 533/559 (95.35%), Query Frame = 0

Query: 8   SNMDALTTTLANSIQALGRGFDVTADIRLLYCKGASGSRLVQLDDGLSHGGDLALSDGVV 67
           S MDALT TL NSIQALGRGFDVTADIRLLYCKG  GSRLVQLDD  +   DL LSDGVV
Sbjct: 17  SEMDALTATLCNSIQALGRGFDVTADIRLLYCKGTPGSRLVQLDDASTR--DLHLSDGVV 76

Query: 68  AFNVPDDVRCSSDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQVD 127
             NVPD V CS D+RATENVPVCSFHKMAE+FNQKSN+SG IPLGSFNAMFNFSGSWQVD
Sbjct: 77  VPNVPDHVHCSPDTRATENVPVCSFHKMAEYFNQKSNLSGNIPLGSFNAMFNFSGSWQVD 136

Query: 128 AAATKSLAMIGYFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSA 187
           AAATKSLAMIGYFIPLFKVELKN  LVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSA
Sbjct: 137 AAATKSLAMIGYFIPLFKVELKNPTLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSA 196

Query: 188 TIGGRDVVYIRQHQSSPLSESEIENYVREVGEQRFLDSKGQSTVGPLSYKDKDVTVIFRR 247
           TIGGRDVVYIRQHQSSPLSESEIENYV+EVGEQRFLDSK QSTVGPLSYKDKDVTVIFRR
Sbjct: 197 TIGGRDVVYIRQHQSSPLSESEIENYVKEVGEQRFLDSKSQSTVGPLSYKDKDVTVIFRR 256

Query: 248 RGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLARAIDLYLEYKPPIED 307
           RGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLE VPG+KHLARAIDLYLEYKPPIED
Sbjct: 257 RGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEEVPGVKHLARAIDLYLEYKPPIED 316

Query: 308 LQYFLDFQVGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLNL 367
           LQYFLDFQ+GRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPDQITVGRKPVTGLRLNL
Sbjct: 317 LQYFLDFQIGRAWAPEQTNLQRKEPLCQSLQFSLMGPKLYISPDQITVGRKPVTGLRLNL 376

Query: 368 EGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVS 427
           EGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGA KWHGPEEQDSRWFEPIKWKNFSHVS
Sbjct: 377 EGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGALKWHGPEEQDSRWFEPIKWKNFSHVS 436

Query: 428 TAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPS 487
           TAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCT+RRSVWDHSPS
Sbjct: 437 TAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTVRRSVWDHSPS 496

Query: 488 TPATPLKSDGASSSSKTRTGEDKKEDSSGNAGKLAKIVDLTEMSKGPQDIPGHWLVSGAK 547
           T ATP +SDGASSSSKT+TGEDKKEDSS NAGKLAKIVDLTEMSKGPQDIPGHWLV+GAK
Sbjct: 497 TSATPSRSDGASSSSKTKTGEDKKEDSSSNAGKLAKIVDLTEMSKGPQDIPGHWLVAGAK 556

Query: 548 LGVDKGRIVLRIKYSLLNY 567
           LGVDKGRIVLRIKYSLLNY
Sbjct: 557 LGVDKGRIVLRIKYSLLNY 573

BLAST of Sed0020662 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 1.2e-252
Identity = 417/556 (75.00%), Postives = 483/556 (86.87%), Query Frame = 0

Query: 11  DALTTTLANSIQALGRGFDVTADIRLLYCKGASGSRLVQLDDGLSHGGDLALSDGVVAFN 70
           +ALTTTL N+IQALGRGFDVT+D+RLLYCKGA GSRLV++++G +   DL LS G +  N
Sbjct: 15  EALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNR--DLELSHGFLLPN 74

Query: 71  VPDDVRCSSDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQVDAAA 130
           VP D+ CS  +  T+ + VCSFH+MAE FN +S + G IPLG FNAMFN++GSWQVDAA+
Sbjct: 75  VPADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAAS 134

Query: 131 TKSLAMIGYFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSATIG 190
           TKSLA++GYFIPL+ V+L    LVLH EI+RAVP +WDP SLASFIEN+GTHI+TS TIG
Sbjct: 135 TKSLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIG 194

Query: 191 GRDVVYIRQHQSSPLSESEIENYVREVGEQRFLDSKGQSTVGPLSYKDKDVTVIFRRRGG 250
           GRDVVYIRQHQSSPL  SEIENYV ++ + RF +++ QS  GPL YKDKD+TVIFRRRGG
Sbjct: 195 GRDVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKDITVIFRRRGG 254

Query: 251 DDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLARAIDLYLEYKPPIEDLQY 310
           DDL QSHA+WAETV +APD+INMTFTPIVS+LEGVPGL+HL RAI+LYLEYKPPIEDLQY
Sbjct: 255 DDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQY 314

Query: 311 FLDFQVGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGC 370
           FLD+Q+ RAWAPEQ+NLQRKEP+CSSLQFSLMGPKL+IS DQ+TVGRKPVTGLRL+LEG 
Sbjct: 315 FLDYQIARAWAPEQSNLQRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSLEGS 374

Query: 371 KQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAP 430
           KQNRL+IHLQHLVSLPKILQPHWDSHV IGAPKW GPEEQDSRWFEPIKWKNFSHVST+P
Sbjct: 375 KQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSHVSTSP 434

Query: 431 IEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPA 490
           IE+TE +IGDLSGVHIVTGAQLGVWDFG+KNVL+LKLLFSKVPGCTIRRSVWDH+P   +
Sbjct: 435 IEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCTIRRSVWDHTPVASS 494

Query: 491 TPLKSDGASSSSKTRTGEDKKEDSSGNAGKLAKIVDLTEMSKGPQDIPGHWLVSGAKLGV 550
             L+  G S+SS T       E+ SG +GKLAKIVD +EM KGPQD+PGHWLV+GAKLGV
Sbjct: 495 GRLEPGGPSTSSST-------EEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGV 554

Query: 551 DKGRIVLRIKYSLLNY 567
           +KG+IVLR+KYSLLNY
Sbjct: 555 EKGKIVLRVKYSLLNY 561

BLAST of Sed0020662 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 1.5e-122
Identity = 251/591 (42.47%), Postives = 353/591 (59.73%), Query Frame = 0

Query: 20  SIQALGRGFDVTADIRLLYCKGAS-GSRLVQLDDGLSHGGDLALSDGVVAFNVPDDVRCS 79
           +I ++G G+D+  D+RL YCKG S  SRL+ + +G     ++ L  G+   NV   ++C 
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEG-DDNCEIVLPGGISIPNVSKSIKCD 73

Query: 80  SDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQVDAAATKSLAMIG 139
              R      +  F +MAE FNQ+ +++G IP G FNAMF FS  WQ DAA TK+LA  G
Sbjct: 74  KGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDG 133

Query: 140 YFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIR 199
            FI L+ V L  S ++L E +K+AVP TWDP +LA FI+ +GTHII S  +GG+DV+Y +
Sbjct: 134 VFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAK 193

Query: 200 QHQSSPLSESEIENYVREVGEQRFLDSK--------------------------GQSTVG 259
           Q  SS L   +++  ++EV ++RF+++                             S++G
Sbjct: 194 QQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLG 253

Query: 260 PLSYKDKDVTVIFRRRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLA 319
             + K+  V +  RR G D+    H +W +TV+  PDVI+M+F PI S+L GVPG   L+
Sbjct: 254 SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLS 313

Query: 320 RAIDLYLEYKPPIEDLQYFLDFQVGRAWAPEQTNL----QRKEPLCSSLQFSLMGPKLYI 379
            AI+LYL YKPPIE+L  FL+FQ+ R WAP  + L    QRK+  C+SLQFS  GPKLY+
Sbjct: 314 HAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKLYV 373

Query: 380 SPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPE 439
           +   + VG++P+TG+RL LEG + NRLAIHLQHL SLPKI Q   D + +I        E
Sbjct: 374 NTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI------RQE 433

Query: 440 EQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLL 499
             D R++E + WKN+SHV T P+E  +    DLS   +VTGAQL V   G KNVL+L+L 
Sbjct: 434 SHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVESHGFKNVLFLRLC 493

Query: 500 FSKVPGCT-IRRSVWDHSPS-TPATPLKSDGASSS-SKTRTGEDKKEDSSGN-------- 559
           FS+V G T ++ S WD +    P + L S   S   +  +    +  D + N        
Sbjct: 494 FSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGP 553

Query: 560 -----AGKLAKIVDLTEMSKGPQDIPGHWLVSGAKLGVDKGRIVLRIKYSL 564
                A KL K VD +EM++GPQ+ PG+W+VSGA+L V+KG+I L++KYSL
Sbjct: 554 PVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of Sed0020662 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 2.1e-108
Identity = 228/597 (38.19%), Postives = 332/597 (55.61%), Query Frame = 0

Query: 20  SIQALGRGFDVTADIRLLYCKGA-SGSRLVQLDDGLSHGGDLALSDGVVAFNVPDDVRCS 79
           ++  +G G+D+ +D+R   CK    GSRLV++D   +   DL    G+V  NV   ++C 
Sbjct: 18  AVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDP--TRNRDLIFPGGIVVNNVSSSIKCD 77

Query: 80  SDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQVDAAATKSLAMIG 139
              R      + SF++M+E FNQ   +SG IP G FN MF FS  W  DA++ K+LA  G
Sbjct: 78  KGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYDG 137

Query: 140 YFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIR 199
           +FI L+ VE+    L L +E+KR VP +WD  +LA FIE +GTH++   T+GG+DV++++
Sbjct: 138 WFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHVK 197

Query: 200 QHQSSPLSESEIENYVREVGEQRF----LDSKGQSTVGP--------------------- 259
           Q + S     EI+  ++  G++RF    ++SK  ++V                       
Sbjct: 198 QMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPFGTSVS 257

Query: 260 ----LSYKDKDVTVIFRRRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLK 319
               +  K++++  +  RRGG DL QSH +W  TV  AP+VI+M F PI S+L G+PG  
Sbjct: 258 SAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPGTG 317

Query: 320 HLARAIDLYLEYKPPIEDLQYFLDFQVGRAWAPE----QTNLQRKEPLCSSLQFSLMGPK 379
            L+ A++LYL YKPPIE+L  FL+FQ+ R WAP        L+R +    SLQFSLMGPK
Sbjct: 318 FLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMGPK 377

Query: 380 LYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQ-PHWDSHVAIGAPKW 439
           LY++  ++  G +PVTGLR  LEG K N LAIHLQHL + P  L   H D++  I  P  
Sbjct: 378 LYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEPVE 437

Query: 440 HGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLY 499
            G       ++ P+KW  FSHV T P++Y  A   D +   IVT A L V   G + VL+
Sbjct: 438 KG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMRKVLF 497

Query: 500 LKLLFSKVPGCTIRRSVWDHSPS--------TPATPLKSDGASSSSKTRTGEDKKEDSSG 559
           L+L FS       R+S WD+  +        +  +   S G S +  T   + K + +S 
Sbjct: 498 LRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKIDINSA 557

Query: 560 ---------NAGKLAKIVDLTEMSKGPQDIPGHWLVSGAKLGVDKGRIVLRIKYSLL 565
                       KL  +VD  E+ +GP++ PG+W+V+GAKL V+ G+I ++ KYSLL
Sbjct: 558 VYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 603

BLAST of Sed0020662 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 8.9e-107
Identity = 240/621 (38.65%), Postives = 339/621 (54.59%), Query Frame = 0

Query: 17  LANSIQALGRGFDVTADIRLLYCKGASGS----RLVQLDDGLSHGGDLALSDGVVAFNVP 76
           +  ++++LG+GFD+TAD RL YCK   GS    RLV LD   +   +L +    V  NV 
Sbjct: 9   IETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQ--TQNRELHIPGFGVFQNVS 68

Query: 77  DDVRCSSDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNF-SGSWQVDAAAT 136
            D+ C    R      +  F+KM+E+FNQ+S+++G IP G+FNA F F SGSW  DAA  
Sbjct: 69  ADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANV 128

Query: 137 KSLAMIGYFIPLFKVELKNSN-LVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSATIG 196
           KSL +    + LF + + N N L L + ++ AVP +WDP  LA FIE +GTH+IT  ++G
Sbjct: 129 KSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVG 188

Query: 197 GRDVVYIRQHQSSPLSESEIENYVREVGEQRF---------------------------- 256
           G+DVV +RQ +SS L    + +++ ++G+Q F                            
Sbjct: 189 GQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAF 248

Query: 257 --LDSKGQSTVGPLSYKDKD-VTVIFRRRGGDDLVQSHAKWAETVKSAPDVINMTFTPIV 316
              D K        S   ++ +TVI  +RGGD   +SH++W  TV   PD IN  F PI 
Sbjct: 249 NVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPIT 308

Query: 317 SMLEGVPGLKHLARAIDLYLEYKPPIEDLQYFLDFQVGRAWAPEQTNL-----QRKEPLC 376
           S+L+ VPG   L+ A+ LYL YKPP+ DLQYFLDF   RAWAP   +L            
Sbjct: 309 SLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAY 368

Query: 377 SSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSL-PKILQPHW 436
            +L  + MGPKLY++   +T  + PVTG+R  LEG K NRLAIHLQHL +    + +   
Sbjct: 369 PALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKIT 428

Query: 437 DSHVAIGAPKWHGPEE--QDSRWFEPIKWKNFSHVSTAPIEY------TEANIGDLSGV- 496
           D H+      W G ++   + R+FEP+  K FSHV T P++Y      T +N    + V 
Sbjct: 429 DEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVA 488

Query: 497 HIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATPLKSDGASSSSKT 556
            IVTGAQL V   G+K+VL+L+L ++KV    + ++ W H P    T  KS   SS S  
Sbjct: 489 FIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPI--GTSQKSGIFSSMSMP 548

Query: 557 RTG---------EDKKE---DSSGNAG--------KLAKIVDLTEMSKGPQDIPGHWLVS 566
            T          +DK E   DS    G        K+ K VDL+++ +GPQ  PGHWLV+
Sbjct: 549 LTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLVT 608

BLAST of Sed0020662 vs. ExPASy TrEMBL
Match: A0A6J1EGA5 (MACPF domain-containing protein CAD1 OS=Cucurbita moschata OX=3662 GN=LOC111433834 PE=4 SV=1)

HSP 1 Score: 1068.9 bits (2763), Expect = 7.2e-309
Identity = 527/560 (94.11%), Postives = 538/560 (96.07%), Query Frame = 0

Query: 7   TSNMDALTTTLANSIQALGRGFDVTADIRLLYCKGASGSRLVQLDDGLSHGGDLALSDGV 66
           TS MDALT TL NSIQALGRGFDVTADIRLLYCKG  GSRLVQLDD  +   DL LSDGV
Sbjct: 16  TSEMDALTATLCNSIQALGRGFDVTADIRLLYCKGTPGSRLVQLDDASTR--DLHLSDGV 75

Query: 67  VAFNVPDDVRCSSDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQV 126
           V  NVPD V CSSD+RATENVPVCSFHKMAE+FNQKSN+SG IPLGSFNAMFNFSGSWQV
Sbjct: 76  VVPNVPDHVHCSSDTRATENVPVCSFHKMAEYFNQKSNLSGNIPLGSFNAMFNFSGSWQV 135

Query: 127 DAAATKSLAMIGYFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITS 186
           DAAATKSLAMIGYFIPLFKVELKNS LVLHEEIKRAVPYTWDPVSLASFIENFGTHIITS
Sbjct: 136 DAAATKSLAMIGYFIPLFKVELKNSTLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITS 195

Query: 187 ATIGGRDVVYIRQHQSSPLSESEIENYVREVGEQRFLDSKGQSTVGPLSYKDKDVTVIFR 246
           ATIGGRDVVYIRQHQSSPLSESEIENYV+EVGEQRFLDSK QSTVGPLSYKDKDVTVIFR
Sbjct: 196 ATIGGRDVVYIRQHQSSPLSESEIENYVKEVGEQRFLDSKSQSTVGPLSYKDKDVTVIFR 255

Query: 247 RRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLARAIDLYLEYKPPIE 306
           RRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPG+KHLARAIDLYLEYKPPIE
Sbjct: 256 RRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIE 315

Query: 307 DLQYFLDFQVGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLN 366
           DLQYFLDFQ+GRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLN
Sbjct: 316 DLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLN 375

Query: 367 LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV 426
           LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGA KWHGPEEQDSRWFEPIKWKNFSHV
Sbjct: 376 LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGALKWHGPEEQDSRWFEPIKWKNFSHV 435

Query: 427 STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP 486
           STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCT+RRSVWDHSP
Sbjct: 436 STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTVRRSVWDHSP 495

Query: 487 STPATPLKSDGASSSSKTRTGEDKKEDSSGNAGKLAKIVDLTEMSKGPQDIPGHWLVSGA 546
           ST ATP +SDGASSSSKT+TGEDKKEDSS NAGKLAKIVDLTEMSKGPQDIPGHWLV+GA
Sbjct: 496 STSATPSRSDGASSSSKTKTGEDKKEDSSSNAGKLAKIVDLTEMSKGPQDIPGHWLVTGA 555

Query: 547 KLGVDKGRIVLRIKYSLLNY 567
           KLGVDKGRIVLRIKYSLLNY
Sbjct: 556 KLGVDKGRIVLRIKYSLLNY 573

BLAST of Sed0020662 vs. ExPASy TrEMBL
Match: A0A6J1CMV2 (MACPF domain-containing protein CAD1 OS=Momordica charantia OX=3673 GN=LOC111012420 PE=4 SV=1)

HSP 1 Score: 1063.1 bits (2748), Expect = 3.9e-307
Identity = 519/560 (92.68%), Postives = 541/560 (96.61%), Query Frame = 0

Query: 7   TSNMDALTTTLANSIQALGRGFDVTADIRLLYCKGASGSRLVQLDDGLSHGGDLALSDGV 66
           TS+MDALT TL+NSIQALGRGFDVTADIRLLYCKG  GSRL+QLDD  +H  DL L+DGV
Sbjct: 18  TSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQLDD--AHTRDLVLTDGV 77

Query: 67  VAFNVPDDVRCSSDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQV 126
           +  NVPDD++CSSD+RATENVPVC+FHKMAE+FNQKS ISG IPLGSF+AMFNFSGSWQV
Sbjct: 78  MVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPLGSFDAMFNFSGSWQV 137

Query: 127 DAAATKSLAMIGYFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITS 186
           DAAATKSLAM+GY IPLFKVELKNSNLVL E+IKRAVPYTWDPVSLASFIENFGTHIITS
Sbjct: 138 DAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITS 197

Query: 187 ATIGGRDVVYIRQHQSSPLSESEIENYVREVGEQRFLDSKGQSTVGPLSYKDKDVTVIFR 246
           ATIGGRDVVYIRQHQSSPLSESEIE+YV+EVGEQRFLDSK QSTVGPLSYKDKDVTVIFR
Sbjct: 198 ATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGPLSYKDKDVTVIFR 257

Query: 247 RRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLARAIDLYLEYKPPIE 306
           RRGGDDLVQSHAKWA+TVK+APDVINMTFTPIVSMLEGVPG+KHLARAIDLYLEYKPPIE
Sbjct: 258 RRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIE 317

Query: 307 DLQYFLDFQVGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLN 366
           DLQYFLDFQ+GRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPDQITVGRKPVTGLRLN
Sbjct: 318 DLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLN 377

Query: 367 LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV 426
           LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV
Sbjct: 378 LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV 437

Query: 427 STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP 486
           STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP
Sbjct: 438 STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP 497

Query: 487 STPATPLKSDGASSSSKTRTGEDKKEDSSGNAGKLAKIVDLTEMSKGPQDIPGHWLVSGA 546
           S PATPL+SDGASSSSKTRT EDKKEDSS NAGKLAKIVDLTEMSKGPQDIPGHWLV+GA
Sbjct: 498 SIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMSKGPQDIPGHWLVTGA 557

Query: 547 KLGVDKGRIVLRIKYSLLNY 567
           KLGVDKGRIVLRIKYSLLNY
Sbjct: 558 KLGVDKGRIVLRIKYSLLNY 575

BLAST of Sed0020662 vs. ExPASy TrEMBL
Match: A0A6J1KQX8 (MACPF domain-containing protein CAD1 OS=Cucurbita maxima OX=3661 GN=LOC111496837 PE=4 SV=1)

HSP 1 Score: 1058.9 bits (2737), Expect = 7.4e-306
Identity = 522/559 (93.38%), Postives = 533/559 (95.35%), Query Frame = 0

Query: 8   SNMDALTTTLANSIQALGRGFDVTADIRLLYCKGASGSRLVQLDDGLSHGGDLALSDGVV 67
           S MDALT TL NSIQALGRGFDVTADIRLLYCKG  GSRLVQLDD  +   DL LSDGVV
Sbjct: 17  SEMDALTATLCNSIQALGRGFDVTADIRLLYCKGTPGSRLVQLDDASTR--DLHLSDGVV 76

Query: 68  AFNVPDDVRCSSDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQVD 127
             NVPD V CS D+RATENVPVCSFHKMAE+FNQKSN+SG IPLGSFNAMFNFSGSWQVD
Sbjct: 77  VPNVPDHVHCSPDTRATENVPVCSFHKMAEYFNQKSNLSGNIPLGSFNAMFNFSGSWQVD 136

Query: 128 AAATKSLAMIGYFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSA 187
           AAATKSLAMIGYFIPLFKVELKN  LVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSA
Sbjct: 137 AAATKSLAMIGYFIPLFKVELKNPTLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSA 196

Query: 188 TIGGRDVVYIRQHQSSPLSESEIENYVREVGEQRFLDSKGQSTVGPLSYKDKDVTVIFRR 247
           TIGGRDVVYIRQHQSSPLSESEIENYV+EVGEQRFLDSK QSTVGPLSYKDKDVTVIFRR
Sbjct: 197 TIGGRDVVYIRQHQSSPLSESEIENYVKEVGEQRFLDSKSQSTVGPLSYKDKDVTVIFRR 256

Query: 248 RGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLARAIDLYLEYKPPIED 307
           RGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLE VPG+KHLARAIDLYLEYKPPIED
Sbjct: 257 RGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEEVPGVKHLARAIDLYLEYKPPIED 316

Query: 308 LQYFLDFQVGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLNL 367
           LQYFLDFQ+GRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPDQITVGRKPVTGLRLNL
Sbjct: 317 LQYFLDFQIGRAWAPEQTNLQRKEPLCQSLQFSLMGPKLYISPDQITVGRKPVTGLRLNL 376

Query: 368 EGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVS 427
           EGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGA KWHGPEEQDSRWFEPIKWKNFSHVS
Sbjct: 377 EGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGALKWHGPEEQDSRWFEPIKWKNFSHVS 436

Query: 428 TAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPS 487
           TAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCT+RRSVWDHSPS
Sbjct: 437 TAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTVRRSVWDHSPS 496

Query: 488 TPATPLKSDGASSSSKTRTGEDKKEDSSGNAGKLAKIVDLTEMSKGPQDIPGHWLVSGAK 547
           T ATP +SDGASSSSKT+TGEDKKEDSS NAGKLAKIVDLTEMSKGPQDIPGHWLV+GAK
Sbjct: 497 TSATPSRSDGASSSSKTKTGEDKKEDSSSNAGKLAKIVDLTEMSKGPQDIPGHWLVAGAK 556

Query: 548 LGVDKGRIVLRIKYSLLNY 567
           LGVDKGRIVLRIKYSLLNY
Sbjct: 557 LGVDKGRIVLRIKYSLLNY 573

BLAST of Sed0020662 vs. ExPASy TrEMBL
Match: A0A5D3CPE6 (MACPF domain-containing protein CAD1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008470 PE=4 SV=1)

HSP 1 Score: 1053.9 bits (2724), Expect = 2.4e-304
Identity = 518/560 (92.50%), Postives = 536/560 (95.71%), Query Frame = 0

Query: 7   TSNMDALTTTLANSIQALGRGFDVTADIRLLYCKGASGSRLVQLDDGLSHGGDLALSDGV 66
           TSNMDALTTTL NSI ALGRGFDVTADIRLLYCKG  GSRLVQLDD  +H  DL LSDGV
Sbjct: 16  TSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLDD--AHTRDLVLSDGV 75

Query: 67  VAFNVPDDVRCSSDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQV 126
           V  NVPDDV+CSSD+RATENVPVCSFHKMAE+FNQKS ISG IPLGSFNAMFNFSGSWQ+
Sbjct: 76  VVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGSFNAMFNFSGSWQL 135

Query: 127 DAAATKSLAMIGYFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITS 186
           DAAATKSLAMIGY+IP FKV LK+SNLVL E+IKRAVPYTWDPVSLASFIENFGTHIITS
Sbjct: 136 DAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITS 195

Query: 187 ATIGGRDVVYIRQHQSSPLSESEIENYVREVGEQRFLDSKGQSTVGPLSYKDKDVTVIFR 246
           ATIGGRDVVYIRQHQSSPLSE EIE+YV+EVGEQRFLDSK QS   PLSYKDKDVTVIFR
Sbjct: 196 ATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAPLSYKDKDVTVIFR 255

Query: 247 RRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLARAIDLYLEYKPPIE 306
           RRGGDDLVQSHA+WA+TVKSAPDVINMTFTPIVSMLEGVPG+KHLARAIDLYLEYKPPIE
Sbjct: 256 RRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIE 315

Query: 307 DLQYFLDFQVGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLN 366
           DLQYFLDFQ+GRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPDQITVGRKPVTGLRLN
Sbjct: 316 DLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLN 375

Query: 367 LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV 426
           LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV
Sbjct: 376 LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV 435

Query: 427 STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP 486
           STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP
Sbjct: 436 STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP 495

Query: 487 STPATPLKSDGASSSSKTRTGEDKKEDSSGNAGKLAKIVDLTEMSKGPQDIPGHWLVSGA 546
           STPATPL+SDGASSSS+TRT EDKKEDSS NAGKLAKIVDLTEMSKGPQDIPGHWLV+GA
Sbjct: 496 STPATPLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGA 555

Query: 547 KLGVDKGRIVLRIKYSLLNY 567
           KLGVDKG+IVLRIKYSLLNY
Sbjct: 556 KLGVDKGKIVLRIKYSLLNY 573

BLAST of Sed0020662 vs. ExPASy TrEMBL
Match: A0A1S3AZC7 (MACPF domain-containing protein CAD1 OS=Cucumis melo OX=3656 GN=LOC103484421 PE=4 SV=1)

HSP 1 Score: 1053.9 bits (2724), Expect = 2.4e-304
Identity = 518/560 (92.50%), Postives = 536/560 (95.71%), Query Frame = 0

Query: 7   TSNMDALTTTLANSIQALGRGFDVTADIRLLYCKGASGSRLVQLDDGLSHGGDLALSDGV 66
           TSNMDALTTTL NSI ALGRGFDVTADIRLLYCKG  GSRLVQLDD  +H  DL LSDGV
Sbjct: 16  TSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLDD--AHTRDLVLSDGV 75

Query: 67  VAFNVPDDVRCSSDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQV 126
           V  NVPDDV+CSSD+RATENVPVCSFHKMAE+FNQKS ISG IPLGSFNAMFNFSGSWQ+
Sbjct: 76  VVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGSFNAMFNFSGSWQL 135

Query: 127 DAAATKSLAMIGYFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITS 186
           DAAATKSLAMIGY+IP FKV LK+SNLVL E+IKRAVPYTWDPVSLASFIENFGTHIITS
Sbjct: 136 DAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITS 195

Query: 187 ATIGGRDVVYIRQHQSSPLSESEIENYVREVGEQRFLDSKGQSTVGPLSYKDKDVTVIFR 246
           ATIGGRDVVYIRQHQSSPLSE EIE+YV+EVGEQRFLDSK QS   PLSYKDKDVTVIFR
Sbjct: 196 ATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAPLSYKDKDVTVIFR 255

Query: 247 RRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLARAIDLYLEYKPPIE 306
           RRGGDDLVQSHA+WA+TVKSAPDVINMTFTPIVSMLEGVPG+KHLARAIDLYLEYKPPIE
Sbjct: 256 RRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIE 315

Query: 307 DLQYFLDFQVGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLN 366
           DLQYFLDFQ+GRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPDQITVGRKPVTGLRLN
Sbjct: 316 DLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLN 375

Query: 367 LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV 426
           LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV
Sbjct: 376 LEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHV 435

Query: 427 STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP 486
           STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP
Sbjct: 436 STAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP 495

Query: 487 STPATPLKSDGASSSSKTRTGEDKKEDSSGNAGKLAKIVDLTEMSKGPQDIPGHWLVSGA 546
           STPATPL+SDGASSSS+TRT EDKKEDSS NAGKLAKIVDLTEMSKGPQDIPGHWLV+GA
Sbjct: 496 STPATPLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGA 555

Query: 547 KLGVDKGRIVLRIKYSLLNY 567
           KLGVDKG+IVLRIKYSLLNY
Sbjct: 556 KLGVDKGKIVLRIKYSLLNY 573

BLAST of Sed0020662 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 873.6 bits (2256), Expect = 8.5e-254
Identity = 417/556 (75.00%), Postives = 483/556 (86.87%), Query Frame = 0

Query: 11  DALTTTLANSIQALGRGFDVTADIRLLYCKGASGSRLVQLDDGLSHGGDLALSDGVVAFN 70
           +ALTTTL N+IQALGRGFDVT+D+RLLYCKGA GSRLV++++G +   DL LS G +  N
Sbjct: 15  EALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNR--DLELSHGFLLPN 74

Query: 71  VPDDVRCSSDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQVDAAA 130
           VP D+ CS  +  T+ + VCSFH+MAE FN +S + G IPLG FNAMFN++GSWQVDAA+
Sbjct: 75  VPADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAAS 134

Query: 131 TKSLAMIGYFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSATIG 190
           TKSLA++GYFIPL+ V+L    LVLH EI+RAVP +WDP SLASFIEN+GTHI+TS TIG
Sbjct: 135 TKSLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIG 194

Query: 191 GRDVVYIRQHQSSPLSESEIENYVREVGEQRFLDSKGQSTVGPLSYKDKDVTVIFRRRGG 250
           GRDVVYIRQHQSSPL  SEIENYV ++ + RF +++ QS  GPL YKDKD+TVIFRRRGG
Sbjct: 195 GRDVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKDITVIFRRRGG 254

Query: 251 DDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLARAIDLYLEYKPPIEDLQY 310
           DDL QSHA+WAETV +APD+INMTFTPIVS+LEGVPGL+HL RAI+LYLEYKPPIEDLQY
Sbjct: 255 DDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQY 314

Query: 311 FLDFQVGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGC 370
           FLD+Q+ RAWAPEQ+NLQRKEP+CSSLQFSLMGPKL+IS DQ+TVGRKPVTGLRL+LEG 
Sbjct: 315 FLDYQIARAWAPEQSNLQRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSLEGS 374

Query: 371 KQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAP 430
           KQNRL+IHLQHLVSLPKILQPHWDSHV IGAPKW GPEEQDSRWFEPIKWKNFSHVST+P
Sbjct: 375 KQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSHVSTSP 434

Query: 431 IEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPA 490
           IE+TE +IGDLSGVHIVTGAQLGVWDFG+KNVL+LKLLFSKVPGCTIRRSVWDH+P   +
Sbjct: 435 IEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCTIRRSVWDHTPVASS 494

Query: 491 TPLKSDGASSSSKTRTGEDKKEDSSGNAGKLAKIVDLTEMSKGPQDIPGHWLVSGAKLGV 550
             L+  G S+SS T       E+ SG +GKLAKIVD +EM KGPQD+PGHWLV+GAKLGV
Sbjct: 495 GRLEPGGPSTSSST-------EEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGV 554

Query: 551 DKGRIVLRIKYSLLNY 567
           +KG+IVLR+KYSLLNY
Sbjct: 555 EKGKIVLRVKYSLLNY 561

BLAST of Sed0020662 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 441.4 bits (1134), Expect = 1.1e-123
Identity = 251/591 (42.47%), Postives = 353/591 (59.73%), Query Frame = 0

Query: 20  SIQALGRGFDVTADIRLLYCKGAS-GSRLVQLDDGLSHGGDLALSDGVVAFNVPDDVRCS 79
           +I ++G G+D+  D+RL YCKG S  SRL+ + +G     ++ L  G+   NV   ++C 
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEG-DDNCEIVLPGGISIPNVSKSIKCD 73

Query: 80  SDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQVDAAATKSLAMIG 139
              R      +  F +MAE FNQ+ +++G IP G FNAMF FS  WQ DAA TK+LA  G
Sbjct: 74  KGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDG 133

Query: 140 YFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIR 199
            FI L+ V L  S ++L E +K+AVP TWDP +LA FI+ +GTHII S  +GG+DV+Y +
Sbjct: 134 VFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAK 193

Query: 200 QHQSSPLSESEIENYVREVGEQRFLDSK--------------------------GQSTVG 259
           Q  SS L   +++  ++EV ++RF+++                             S++G
Sbjct: 194 QQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLG 253

Query: 260 PLSYKDKDVTVIFRRRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLA 319
             + K+  V +  RR G D+    H +W +TV+  PDVI+M+F PI S+L GVPG   L+
Sbjct: 254 SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLS 313

Query: 320 RAIDLYLEYKPPIEDLQYFLDFQVGRAWAPEQTNL----QRKEPLCSSLQFSLMGPKLYI 379
            AI+LYL YKPPIE+L  FL+FQ+ R WAP  + L    QRK+  C+SLQFS  GPKLY+
Sbjct: 314 HAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKLYV 373

Query: 380 SPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPE 439
           +   + VG++P+TG+RL LEG + NRLAIHLQHL SLPKI Q   D + +I        E
Sbjct: 374 NTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI------RQE 433

Query: 440 EQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLL 499
             D R++E + WKN+SHV T P+E  +    DLS   +VTGAQL V   G KNVL+L+L 
Sbjct: 434 SHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVESHGFKNVLFLRLC 493

Query: 500 FSKVPGCT-IRRSVWDHSPS-TPATPLKSDGASSS-SKTRTGEDKKEDSSGN-------- 559
           FS+V G T ++ S WD +    P + L S   S   +  +    +  D + N        
Sbjct: 494 FSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGP 553

Query: 560 -----AGKLAKIVDLTEMSKGPQDIPGHWLVSGAKLGVDKGRIVLRIKYSL 564
                A KL K VD +EM++GPQ+ PG+W+VSGA+L V+KG+I L++KYSL
Sbjct: 554 PVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of Sed0020662 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 394.4 bits (1012), Expect = 1.5e-109
Identity = 228/597 (38.19%), Postives = 332/597 (55.61%), Query Frame = 0

Query: 20  SIQALGRGFDVTADIRLLYCKGA-SGSRLVQLDDGLSHGGDLALSDGVVAFNVPDDVRCS 79
           ++  +G G+D+ +D+R   CK    GSRLV++D   +   DL    G+V  NV   ++C 
Sbjct: 18  AVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDP--TRNRDLIFPGGIVVNNVSSSIKCD 77

Query: 80  SDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQVDAAATKSLAMIG 139
              R      + SF++M+E FNQ   +SG IP G FN MF FS  W  DA++ K+LA  G
Sbjct: 78  KGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYDG 137

Query: 140 YFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIR 199
           +FI L+ VE+    L L +E+KR VP +WD  +LA FIE +GTH++   T+GG+DV++++
Sbjct: 138 WFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHVK 197

Query: 200 QHQSSPLSESEIENYVREVGEQRF----LDSKGQSTVGP--------------------- 259
           Q + S     EI+  ++  G++RF    ++SK  ++V                       
Sbjct: 198 QMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPFGTSVS 257

Query: 260 ----LSYKDKDVTVIFRRRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLK 319
               +  K++++  +  RRGG DL QSH +W  TV  AP+VI+M F PI S+L G+PG  
Sbjct: 258 SAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPGTG 317

Query: 320 HLARAIDLYLEYKPPIEDLQYFLDFQVGRAWAPE----QTNLQRKEPLCSSLQFSLMGPK 379
            L+ A++LYL YKPPIE+L  FL+FQ+ R WAP        L+R +    SLQFSLMGPK
Sbjct: 318 FLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMGPK 377

Query: 380 LYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQ-PHWDSHVAIGAPKW 439
           LY++  ++  G +PVTGLR  LEG K N LAIHLQHL + P  L   H D++  I  P  
Sbjct: 378 LYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEPVE 437

Query: 440 HGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLY 499
            G       ++ P+KW  FSHV T P++Y  A   D +   IVT A L V   G + VL+
Sbjct: 438 KG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMRKVLF 497

Query: 500 LKLLFSKVPGCTIRRSVWDHSPS--------TPATPLKSDGASSSSKTRTGEDKKEDSSG 559
           L+L FS       R+S WD+  +        +  +   S G S +  T   + K + +S 
Sbjct: 498 LRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKIDINSA 557

Query: 560 ---------NAGKLAKIVDLTEMSKGPQDIPGHWLVSGAKLGVDKGRIVLRIKYSLL 565
                       KL  +VD  E+ +GP++ PG+W+V+GAKL V+ G+I ++ KYSLL
Sbjct: 558 VYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 603

BLAST of Sed0020662 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 389.0 bits (998), Expect = 6.3e-108
Identity = 240/621 (38.65%), Postives = 339/621 (54.59%), Query Frame = 0

Query: 17  LANSIQALGRGFDVTADIRLLYCKGASGS----RLVQLDDGLSHGGDLALSDGVVAFNVP 76
           +  ++++LG+GFD+TAD RL YCK   GS    RLV LD   +   +L +    V  NV 
Sbjct: 9   IETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQ--TQNRELHIPGFGVFQNVS 68

Query: 77  DDVRCSSDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNF-SGSWQVDAAAT 136
            D+ C    R      +  F+KM+E+FNQ+S+++G IP G+FNA F F SGSW  DAA  
Sbjct: 69  ADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANV 128

Query: 137 KSLAMIGYFIPLFKVELKNSN-LVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSATIG 196
           KSL +    + LF + + N N L L + ++ AVP +WDP  LA FIE +GTH+IT  ++G
Sbjct: 129 KSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVG 188

Query: 197 GRDVVYIRQHQSSPLSESEIENYVREVGEQRF---------------------------- 256
           G+DVV +RQ +SS L    + +++ ++G+Q F                            
Sbjct: 189 GQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAF 248

Query: 257 --LDSKGQSTVGPLSYKDKD-VTVIFRRRGGDDLVQSHAKWAETVKSAPDVINMTFTPIV 316
              D K        S   ++ +TVI  +RGGD   +SH++W  TV   PD IN  F PI 
Sbjct: 249 NVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPIT 308

Query: 317 SMLEGVPGLKHLARAIDLYLEYKPPIEDLQYFLDFQVGRAWAPEQTNL-----QRKEPLC 376
           S+L+ VPG   L+ A+ LYL YKPP+ DLQYFLDF   RAWAP   +L            
Sbjct: 309 SLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAY 368

Query: 377 SSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSL-PKILQPHW 436
            +L  + MGPKLY++   +T  + PVTG+R  LEG K NRLAIHLQHL +    + +   
Sbjct: 369 PALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKIT 428

Query: 437 DSHVAIGAPKWHGPEE--QDSRWFEPIKWKNFSHVSTAPIEY------TEANIGDLSGV- 496
           D H+      W G ++   + R+FEP+  K FSHV T P++Y      T +N    + V 
Sbjct: 429 DEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVA 488

Query: 497 HIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATPLKSDGASSSSKT 556
            IVTGAQL V   G+K+VL+L+L ++KV    + ++ W H P    T  KS   SS S  
Sbjct: 489 FIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPI--GTSQKSGIFSSMSMP 548

Query: 557 RTG---------EDKKE---DSSGNAG--------KLAKIVDLTEMSKGPQDIPGHWLVS 566
            T          +DK E   DS    G        K+ K VDL+++ +GPQ  PGHWLV+
Sbjct: 549 LTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLVT 608

BLAST of Sed0020662 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 231.1 bits (588), Expect = 2.2e-60
Identity = 123/311 (39.55%), Postives = 180/311 (57.88%), Query Frame = 0

Query: 20  SIQALGRGFDVTADIRLLYCKGAS-GSRLVQLDDGLSHGGDLALSDGVVAFNVPDDVRCS 79
           +I ++G G+D+  D+RL YCKG S  SRL+ + +G     ++ L  G+   NV   ++C 
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEG-DDNCEIVLPGGISIPNVSKSIKCD 73

Query: 80  SDSRATENVPVCSFHKMAEHFNQKSNISGTIPLGSFNAMFNFSGSWQVDAAATKSLAMIG 139
              R      +  F +MAE FNQ+ +++G IP G FNAMF FS  WQ DAA TK+LA  G
Sbjct: 74  KGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDG 133

Query: 140 YFIPLFKVELKNSNLVLHEEIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIR 199
            FI L+ V L  S ++L E +K+AVP TWDP +LA FI+ +GTHII S  +GG+DV+Y +
Sbjct: 134 VFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAK 193

Query: 200 QHQSSPLSESEIENYVREVGEQRFLDSK--------------------------GQSTVG 259
           Q  SS L   +++  ++EV ++RF+++                             S++G
Sbjct: 194 QQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLG 253

Query: 260 PLSYKDKDVTVIFRRRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGLKHLA 304
             + K+  V +  RR G D+    H +W +TV+  PDVI+M+F PI S+L GVPG   L+
Sbjct: 254 SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLS 313