Homology
BLAST of Sed0020084 vs. NCBI nr
Match:
XP_038898390.1 (ent-kaurene oxidase [Benincasa hispida])
HSP 1 Score: 901.0 bits (2327), Expect = 4.9e-258
Identity = 443/519 (85.36%), Postives = 482/519 (92.87%), Query Frame = 0
Query: 1 MAIVTDPLGSVQNLVLALHAPPYTAAAVLGAVSFLVYVLLIGGWRKRRRDLLPPVPAIPG 60
MA+VTDPL S+Q L L APPY AAAVLG VS ++ V L+ RK+RR+ LPPVPAIPG
Sbjct: 1 MAVVTDPLTSMQLLANTLQAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAIPG 60
Query: 61 VPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEVAKEAMVTRYSSIS 120
VP+LGNLLQLKEKKPH TFA+WA+ YGPVYSI+TGASTVIVLNTAEVAKEAMVTRY+SIS
Sbjct: 61 VPVLGNLLQLKEKKPHMTFARWAEKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYASIS 120
Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQKRHRQRRDTMIGNISS 180
SRKLSKALTILTADKCMVAMSDYNDFHK+VK+YILAN+LGANAQK+HRQRRDTMI NIS
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANMLGANAQKKHRQRRDTMIENISR 180
Query: 181 QLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVDGLGTTLPREDLFRVLVL 240
+LFAHVK+ PS SVNFR IFESELF+LALKETLGKDI+SIYVDGLGTTLPREDLFRVLV+
Sbjct: 181 ELFAHVKEFPSESVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240
Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKSLIEEHKKRIVAGEETNC 300
DPMEGAIEVDWRDFFPYLRWIPNKSVE+KI++M F+R+MTM+SLIEE KKRI AGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRKMDFQRQMTMRSLIEEQKKRIAAGEETNC 300
Query: 301 YADFLLSEAKTLTEDQITMLLWETIIETSDTTLVVTEWAMYELSKHTKRQDYLYKHIQRV 360
YADFLLSEAKTLTEDQI+MLLWETIIETSDTTLVVTEWAMYEL+K KRQD+LY+HI
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELAKDPKRQDFLYQHILSA 360
Query: 361 CGSATLTEENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQEDTQLGGFFVPAGSEIAVNI 420
CGS+ LTEENLSQLPYLTAVFHETLR HSPVPVVPLRY EDTQLGGFFVPAGSEIAVNI
Sbjct: 361 CGSSKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
Query: 421 YACNMDEEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGRRVCAGALKAMLIACTTIGR 480
YACNMD++HWESPEEWKPERFLDEKYDPMDLHKTMAFGGG+RVCAGALKAMLIACTTIGR
Sbjct: 421 YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480
Query: 481 LVQEFEWKLKGGEEDKVDTLGLTARKLQPLHAIIKPRIN 520
+ QEFEWKL+ GEEDKVDTLGLTARKLQPLH ++KPRIN
Sbjct: 481 MAQEFEWKLREGEEDKVDTLGLTARKLQPLHVVVKPRIN 519
BLAST of Sed0020084 vs. NCBI nr
Match:
XP_008452488.1 (PREDICTED: ent-kaurene oxidase, chloroplastic [Cucumis melo] >KAA0064350.1 ent-kaurene oxidase [Cucumis melo var. makuwa] >TYK20237.1 ent-kaurene oxidase [Cucumis melo var. makuwa])
HSP 1 Score: 899.8 bits (2324), Expect = 1.1e-257
Identity = 442/519 (85.16%), Postives = 481/519 (92.68%), Query Frame = 0
Query: 1 MAIVTDPLGSVQNLVLALHAPPYTAAAVLGAVSFLVYVLLIGGWRKRRRDLLPPVPAIPG 60
MA+VTDPL S+Q L L APPY AAAVLG VS ++ V L+ RK+RR+ LPPVPA+PG
Sbjct: 1 MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAVPG 60
Query: 61 VPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEVAKEAMVTRYSSIS 120
VPLLGNLLQLKEKKPHKTFA+WA+TYGPVYSI+TGASTVIVLNT EVAKEAMVTRY SIS
Sbjct: 61 VPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120
Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQKRHRQRRDTMIGNISS 180
SRKLSKALTILTADKCMVAMSDYN+FHK+VK+YILANVLGANAQK+HRQRRD MI NIS
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180
Query: 181 QLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVDGLGTTLPREDLFRVLVL 240
+LFAHVK+ PS +VNFR IFESELF+LALKETLGKDI+SIYVDGLGTTLPREDLFR+LV+
Sbjct: 181 ELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240
Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKSLIEEHKKRIVAGEETNC 300
DPMEGAIEVDWRDFFPYLRWIPNKSVE+KI+ M FRR+MTMKSLI+E K+RI AGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTMKSLIQEPKRRIAAGEETNC 300
Query: 301 YADFLLSEAKTLTEDQITMLLWETIIETSDTTLVVTEWAMYELSKHTKRQDYLYKHIQRV 360
YADFLLSEAKTLTEDQI+MLLWETIIETSDTTLVVTEWAMYELSK KRQDYLY+ IQ V
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPKRQDYLYQQIQSV 360
Query: 361 CGSATLTEENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQEDTQLGGFFVPAGSEIAVNI 420
CGS+TLTEENLSQLPYLTA+FHETLR HSPVPVVPLRY EDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420
Query: 421 YACNMDEEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGRRVCAGALKAMLIACTTIGR 480
YACNMD+ HWE+PEEWKPERFLDEKYDPMDLHKTMAFGGG+RVCAGALKAMLIACTTIGR
Sbjct: 421 YACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480
Query: 481 LVQEFEWKLKGGEEDKVDTLGLTARKLQPLHAIIKPRIN 520
+VQEFEWKL+ GEE+KVDTLGLTARKLQPLH +IKPR N
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519
BLAST of Sed0020084 vs. NCBI nr
Match:
XP_022139822.1 (ent-kaurene oxidase, chloroplastic [Momordica charantia] >XP_022139833.1 ent-kaurene oxidase, chloroplastic [Momordica charantia])
HSP 1 Score: 896.0 bits (2314), Expect = 1.6e-256
Identity = 442/519 (85.16%), Postives = 475/519 (91.52%), Query Frame = 0
Query: 1 MAIVTDPLGSVQNLVLALHAPPYTAAAVLGAVSFLVYVLLIGGWRKRRRDLLPPVPAIPG 60
MAI +D L S+Q L+ +LHAPPY AAAVLG VS L+ V +IGGWRKR R+LLPPVPAIPG
Sbjct: 1 MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60
Query: 61 VPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEVAKEAMVTRYSSIS 120
+P+LGNLLQLKEKKPHKTFA+WA YGPVYSI+TGASTVIVLNTAEVAKEAMVTRYSSIS
Sbjct: 61 LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120
Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQKRHRQRRDTMIGNISS 180
SRKLSKALTILT+DKCMVAMSDYNDFHK+ K+YILANVLGANAQKRHRQRRDTMI NISS
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISS 180
Query: 181 QLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVDGLGTTLPREDLFRVLVL 240
LFAH KD+PS SVNFR IFE ELF+LALKETLG DI+SIYV LGTT REDLFR+LV+
Sbjct: 181 HLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIESIYVSELGTTALREDLFRILVI 240
Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKSLIEEHKKRIVAGEETNC 300
DPMEGAIEVDWRDFFPYLRWIPNK VEDKI+ M +RR++TMKSLIEE KKRI AGEETN
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNS 300
Query: 301 YADFLLSEAKTLTEDQITMLLWETIIETSDTTLVVTEWAMYELSKHTKRQDYLYKHIQRV 360
YA+FLLSEAKTLTEDQI+MLLWETIIETSDTTLVVTEWAMYEL+K KRQDYLY+HI V
Sbjct: 301 YAEFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELAKDPKRQDYLYQHILSV 360
Query: 361 CGSATLTEENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQEDTQLGGFFVPAGSEIAVNI 420
CGSA L EENL+QLPYLTAVFHETLR HSPVP+VPLRY EDTQLGGFFVPAGSEIAVNI
Sbjct: 361 CGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
Query: 421 YACNMDEEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGRRVCAGALKAMLIACTTIGR 480
Y CNMD+EHWESPEEWKPERFLDEKYDPMDLHKTMAFGGG+RVCAGALKA L+ACTTIGR
Sbjct: 421 YGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGR 480
Query: 481 LVQEFEWKLKGGEEDKVDTLGLTARKLQPLHAIIKPRIN 520
LVQEFEWKL GGEEDKVDTLGLTARKLQPLHAI+KPR N
Sbjct: 481 LVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN 519
BLAST of Sed0020084 vs. NCBI nr
Match:
XP_022976063.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima])
HSP 1 Score: 895.2 bits (2312), Expect = 2.7e-256
Identity = 439/519 (84.59%), Postives = 482/519 (92.87%), Query Frame = 0
Query: 1 MAIVTDPLGSVQNLVLALHAPPYTAAAVLGAVSFLVYVLLIGGWRKRRRDLLPPVPAIPG 60
MAI DPLGS+Q+L+ L APPY AAA+LG +SFL+ L+ WRK RR+ LPPVPAIPG
Sbjct: 1 MAIFNDPLGSLQHLLDTLQAPPYAAAAILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60
Query: 61 VPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEVAKEAMVTRYSSIS 120
+P+LGNLLQLKEKKPHKTFA+WA+TYGP+YSI+TGASTVIVLNTAEVAKEAMVTRY+S+S
Sbjct: 61 LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120
Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQKRHRQRRDTMIGNISS 180
RKLSKALTILT+DKCMVAMSDYNDFHK+VKKYILANVLGANAQKRHRQRRD+MI NISS
Sbjct: 121 GRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDSMIDNISS 180
Query: 181 QLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVDGLGTTLPREDLFRVLVL 240
QLFAHVKD+PS SVNFR IFESELF LALKETLGKDI+SIYVDGLGTTLPREDLFRVLV+
Sbjct: 181 QLFAHVKDSPSESVNFRKIFESELFLLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240
Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKSLIEEHKKRIVAGEETNC 300
D MEGAIEVDWRDFFPYL+WIPNKSVE+KI++M +RR++TM SLIEE KKRI AGEETNC
Sbjct: 241 DLMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDYRRRVTMMSLIEEQKKRIAAGEETNC 300
Query: 301 YADFLLSEAKTLTEDQITMLLWETIIETSDTTLVVTEWAMYELSKHTKRQDYLYKHIQRV 360
YADFLLSEAKTLTE QI MLLWETIIETSDTTLVVTEWAMYEL+K+ +RQDYLY+HIQ V
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPERQDYLYQHIQSV 360
Query: 361 CGSATLTEENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQEDTQLGGFFVPAGSEIAVNI 420
CGS LTE++LS+LPYLTAVFHETLR HSPVP+VPLRYV ED QLGGFFVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420
Query: 421 YACNMDEEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGRRVCAGALKAMLIACTTIGR 480
YACNMD++ WESPEEWKPERFLDEKYDPMDLHKTMAFGGG+RVCAGALKAMLIACT+IGR
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480
Query: 481 LVQEFEWKLKGGEEDKVDTLGLTARKLQPLHAIIKPRIN 520
LVQEFEWKL GEEDKVDTLGLTARKLQPLH I+KPRI+
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHVIVKPRIS 519
BLAST of Sed0020084 vs. NCBI nr
Match:
XP_023536271.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 894.8 bits (2311), Expect = 3.5e-256
Identity = 439/519 (84.59%), Postives = 482/519 (92.87%), Query Frame = 0
Query: 1 MAIVTDPLGSVQNLVLALHAPPYTAAAVLGAVSFLVYVLLIGGWRKRRRDLLPPVPAIPG 60
MAI DPLGS+Q+L+ L AP Y AAA+LG +SFL+ + WRK RR+ LPPVPAIPG
Sbjct: 1 MAIFNDPLGSLQHLLDTLQAPTYAAAAILGVISFLLAGFFVADWRKSRRNFLPPVPAIPG 60
Query: 61 VPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEVAKEAMVTRYSSIS 120
VPLLGNLLQLKEKKPHKTFA+WA+ YGP+YSI+TGASTVIVLNTAEVAKEAMVTR++S+S
Sbjct: 61 VPLLGNLLQLKEKKPHKTFARWAERYGPIYSIRTGASTVIVLNTAEVAKEAMVTRHASMS 120
Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQKRHRQRRDTMIGNISS 180
SRKLSKALTILT+DKCMVAMSDYNDFHK+VKKYILANVLGANAQKRHRQRRD+MI NISS
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDSMIDNISS 180
Query: 181 QLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVDGLGTTLPREDLFRVLVL 240
QLFAHVKD+PS SVNFR IFESELF+LALKET+GKDI+SIYVDGL TTLPREDLFRVLV+
Sbjct: 181 QLFAHVKDSPSESVNFRKIFESELFRLALKETVGKDIESIYVDGLSTTLPREDLFRVLVI 240
Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKSLIEEHKKRIVAGEETNC 300
DPMEGAIEVDWRDFFPYL+WIPNKSVE+KI++M FRR++TM SLIEE KKRI AGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDFRRRVTMMSLIEEQKKRIAAGEETNC 300
Query: 301 YADFLLSEAKTLTEDQITMLLWETIIETSDTTLVVTEWAMYELSKHTKRQDYLYKHIQRV 360
YADFLLSEAKTLTE QI MLLWETIIETSDTTLVVTEWAMYEL+K+ KRQDYLY+HIQ V
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPKRQDYLYQHIQSV 360
Query: 361 CGSATLTEENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQEDTQLGGFFVPAGSEIAVNI 420
CGS LTE++LS+LPYLTAVFHETLR HSPVP+VPLRYV ED QLGGFFVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420
Query: 421 YACNMDEEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGRRVCAGALKAMLIACTTIGR 480
YACNMD++ WESPEEWKPERFLD+KYDPMDLHKTMAFGGG+RVCAGALKAMLIACT+IGR
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480
Query: 481 LVQEFEWKLKGGEEDKVDTLGLTARKLQPLHAIIKPRIN 520
LVQEFEWKL GEEDKVDTLGLTARKLQPLHAI+KPRI+
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHAIVKPRIS 519
BLAST of Sed0020084 vs. ExPASy Swiss-Prot
Match:
A0A0G2RKY1 (Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1)
HSP 1 Score: 648.7 bits (1672), Expect = 5.7e-185
Identity = 318/510 (62.35%), Postives = 395/510 (77.45%), Query Frame = 0
Query: 15 VLALHAPPYTAA-----AVLGAVSFLVYVLLIGGWRKRRRDLLPPVPAIPGVPLLGNLLQ 74
+L+L A P AA +G ++ + RK+ PP P +PG+P++GNLLQ
Sbjct: 4 LLSLQAVPAAAAIGGPVVAIGGITLFFIREYVKDQRKKSSS-FPPPPKVPGLPVIGNLLQ 63
Query: 75 LKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEVAKEAMVTRYSSISSRKLSKALT 134
LKEKKPHKTF +W++ YGP+YSI+TGA+T+IVLNT +VAKEAM+T+YSSIS+RKLSKALT
Sbjct: 64 LKEKKPHKTFTKWSEKYGPIYSIRTGANTMIVLNTNDVAKEAMITKYSSISTRKLSKALT 123
Query: 135 ILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQKRHRQRRDTMIGNISSQLFAHVKDT 194
ILT+DK +VAMSDYN+F+K K+++L + LG AQKRHR R+ MI NI Q AH K
Sbjct: 124 ILTSDKSIVAMSDYNEFYKAAKRHLLTSTLGPTAQKRHRVHRNLMINNICDQFLAHAKMY 183
Query: 195 PSASVNFRNIFESELFQLALKETLGKDIDSIYVDGLGTTLPREDLFRVLVLDPMEGAIEV 254
PS +VNFR IF+SELF L++K+ +G+D++SIYV+ L TTL R+++F++LV+DPMEGAI+V
Sbjct: 184 PSEAVNFRKIFQSELFGLSMKQAIGEDVESIYVEDLDTTLSRQEMFKILVVDPMEGAIDV 243
Query: 255 DWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKSLIEEHKKRIVAGEETNCYADFLLSE- 314
DWRDFFPYL+WIPN+ E+KI++M+F R+ MK+LIE+ KKRI +G+ NCY D LLSE
Sbjct: 244 DWRDFFPYLKWIPNQHFENKIQQMHFHREAVMKALIEQQKKRIASGKAINCYLDHLLSEA 303
Query: 315 AKTLTEDQITMLLWETIIETSDTTLVVTEWAMYELSKHTKRQDYLYKHIQRVCGSATLTE 374
A TL+E QI MLLWE IIE SDTTLV TEWAMYELSK KRQ+YL IQ CG L E
Sbjct: 304 ADTLSEQQILMLLWEAIIEASDTTLVTTEWAMYELSKDPKRQNYLLSEIQNACGFDQLNE 363
Query: 375 ENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQEDTQLGGFFVPAGSEIAVNIYACNMDEE 434
E L +LPYL A+F ETLR HSPVPVVPLRYV E+TQLGG+ +P GSEIA+NIY CNMD+
Sbjct: 364 EKLCRLPYLAAIFQETLRKHSPVPVVPLRYVHEETQLGGYTIPEGSEIAINIYGCNMDKN 423
Query: 435 HWESPEEWKPERFLDEKYDPMDLHKTMAFGGGRRVCAGALKAMLIACTTIGRLVQEFEWK 494
W+SPEEW+PERF+ K D +LHKTMAFGGG+RVCAGAL+AM I+C IGRLVQE EW+
Sbjct: 424 VWDSPEEWRPERFVFGKDDTTELHKTMAFGGGKRVCAGALQAMTISCIAIGRLVQELEWR 483
Query: 495 LKGGEEDKVDTLGLTARKLQPLHAIIKPRI 519
L GEE VDTLGLT KL PL IIKPR+
Sbjct: 484 LGDGEEANVDTLGLTTHKLHPLQTIIKPRL 512
BLAST of Sed0020084 vs. ExPASy Swiss-Prot
Match:
Q93ZB2 (Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV=2)
HSP 1 Score: 624.0 bits (1608), Expect = 1.5e-177
Identity = 302/467 (64.67%), Postives = 374/467 (80.09%), Query Frame = 0
Query: 52 LPPVPAIPGVPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEVAKEA 111
LP VP +PG P++GNLLQLKEKKPHKTF +W++ YGP+YSIK G+S++IVLN+ E AKEA
Sbjct: 41 LPSVPVVPGFPVIGNLLQLKEKKPHKTFTRWSEIYGPIYSIKMGSSSLIVLNSTETAKEA 100
Query: 112 MVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQKRHRQRR 171
MVTR+SSIS+RKLS ALT+LT DK MVA SDY+DFHKLVK+ +L +LGANAQKR R R
Sbjct: 101 MVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYR 160
Query: 172 DTMIGNISSQLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVDGLGTTLPR 231
D +I N+SS+L AH +D P VNFR IFE ELF +ALK+ GKD++SIYV LG TL +
Sbjct: 161 DALIENVSSKLHAHARDHPQEPVNFRAIFEHELFGVALKQAFGKDVESIYVKELGVTLSK 220
Query: 232 EDLFRVLVLDPMEGAIEVDWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKSLIEEHKKR 291
+++F+VLV D MEGAI+VDWRDFFPYL+WIPNKS E +I++ + RR M +LI++ K+
Sbjct: 221 DEIFKVLVHDMMEGAIDVDWRDFFPYLKWIPNKSFEARIQQKHKRRLAVMNALIQDRLKQ 280
Query: 292 IVAGEETNCYADFLLSEAKTLTEDQITMLLWETIIETSDTTLVVTEWAMYELSKHTKRQD 351
+ + +CY +FL+SEAKTLT++QI +L+WETIIET+DTTLV TEWA+YEL+KH QD
Sbjct: 281 NGSESDDDCYLNFLMSEAKTLTKEQIAILVWETIIETADTTLVTTEWAIYELAKHPSVQD 340
Query: 352 YLYKHIQRVCGSATLTEENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQEDTQLGGFFVP 411
L K IQ VCG EE LSQ+PYL VFHETLR +SP P+VP+RY EDTQ+GG+ VP
Sbjct: 341 RLCKEIQNVCGGEKFKEEQLSQVPYLNGVFHETLRKYSPAPLVPIRYAHEDTQIGGYHVP 400
Query: 412 AGSEIAVNIYACNMDEEHWESPEEWKPERFLDE-KYDPMDLHKTMAFGGGRRVCAGALKA 471
AGSEIA+NIY CNMD++ WE PE+W PERFLD+ KY+ DLHKTMAFG G+RVCAGAL+A
Sbjct: 401 AGSEIAINIYGCNMDKKRWERPEDWWPERFLDDGKYETSDLHKTMAFGAGKRVCAGALQA 460
Query: 472 MLIACTTIGRLVQEFEWKLKGGEEDKVDTLGLTARKLQPLHAIIKPR 518
L+A IGRLVQEFEWKL+ GEE+ VDT GLT++KL PL AII PR
Sbjct: 461 SLMAGIAIGRLVQEFEWKLRDGEEENVDTYGLTSQKLYPLMAIINPR 507
BLAST of Sed0020084 vs. ExPASy Swiss-Prot
Match:
Q5Z5R4 (Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 SV=1)
HSP 1 Score: 569.3 bits (1466), Expect = 4.4e-161
Identity = 276/493 (55.98%), Postives = 370/493 (75.05%), Query Frame = 0
Query: 25 AAAVLGAVSFLVYVLLIGGWRKRRRDLLPPVPAIPGVPLLGNLLQLKEKKPHKTFAQWAK 84
AAAV G V+ G R+R P PA+PG+P++GNL QLKEKKPH+TFA+WA+
Sbjct: 13 AAAVGGFVAAAALAERAGVIAPRKRPNAP--PAVPGLPIIGNLHQLKEKKPHQTFAKWAE 72
Query: 85 TYGPVYSIKTGASTVIVLNTAEVAKEAMVTRYSSISSRKLSKALTILTADKCMVAMSDYN 144
YGP+Y+I+TGAS+V+VLN+ EVAKEAMV ++SSIS+RKLSKALT+LT DK MVA SDY
Sbjct: 73 IYGPIYTIRTGASSVVVLNSTEVAKEAMVAKFSSISTRKLSKALTVLTRDKSMVATSDYC 132
Query: 145 DFHKLVKKYILANVLGANAQKRHRQRRDTMIGNISSQLFAHVKDTPSASVNFRNIFESEL 204
DFHK+VK+Y+++++LG +AQK+ R RD MI N+ S VKD P A + FR++F+ EL
Sbjct: 133 DFHKMVKRYVMSSMLGTSAQKQFRDIRDMMIHNMLSTFHKLVKDDPHAPLIFRDVFKDEL 192
Query: 205 FQLALKETLGKDIDSIYVDGLGTTLPREDLFRVLVLDPMEGAIEVDWRDFFPYLRWIPNK 264
F+L++ ++LG+D+ S+YVD G + +E+++ V D M AIEVDWRDFFPYL W+PNK
Sbjct: 193 FRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNATVTDMMMCAIEVDWRDFFPYLSWVPNK 252
Query: 265 SVEDKIKRMYFRRKMTMKSLIEEHKKRIVAGEETNCYADFLLSEAKTLTEDQITMLLWET 324
S E ++ RR M++LI++ K+RIV GE CY DFLL+E TLT++Q+ ML+WE
Sbjct: 253 SFETRVFTTETRRTAVMRALIKQQKERIVRGEAKTCYLDFLLAE-NTLTDEQLMMLVWEA 312
Query: 325 IIETSDTTLVVTEWAMYELSKHTKRQDYLYKHIQRVCGSATLTEENLSQLPYLTAVFHET 384
+IE +DTTLV TEWAMYEL+K+ +Q+ LY+ I+ VCG T+TEE+L +LPYL AVFHET
Sbjct: 313 LIEAADTTLVTTEWAMYELAKNPDKQERLYQEIREVCGDETVTEEHLPRLPYLNAVFHET 372
Query: 385 LRLHSPVPVVPLRYVQEDTQLGGFFVPAGSEIAVNIYACNMDEEHWESPEEWKPERFLDE 444
LR HSPVP++P R+V EDT+L G+ VPAG+E+ +N+Y CNM+ + WESPEEW PERF
Sbjct: 373 LRRHSPVPLIPPRFVHEDTKLAGYDVPAGTEMVINLYGCNMNRKEWESPEEWVPERFAGG 432
Query: 445 KYDPMDLHKTMAFGGGRRVCAGALKAMLIACTTIGRLVQEFEWKLKGGEEDKVDTLGLTA 504
+ + D++KTMAFG GRR CAG+L+A IAC + R VQEF W+L+ G+E+KVDT+ LTA
Sbjct: 433 RLEVADMYKTMAFGAGRRACAGSLQATHIACAAVARFVQEFGWRLREGDEEKVDTVQLTA 492
Query: 505 RKLQPLHAIIKPR 518
KL PLH + R
Sbjct: 493 YKLHPLHVHLTRR 502
BLAST of Sed0020084 vs. ExPASy Swiss-Prot
Match:
Q5Z5R7 (Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19 PE=2 SV=1)
HSP 1 Score: 562.0 bits (1447), Expect = 7.0e-159
Identity = 273/493 (55.38%), Postives = 366/493 (74.24%), Query Frame = 0
Query: 25 AAAVLGAVSFLVYVLLIGGWRKRRRDLLPPVPAIPGVPLLGNLLQLKEKKPHKTFAQWAK 84
AAA +G F+ L K RR+ P PA+PG+P++GNL QLKEKKPH+TF +WA+
Sbjct: 15 AAAAVG--GFIAAATLAVAPPKNRRN---PPPAVPGLPIIGNLHQLKEKKPHQTFTKWAE 74
Query: 85 TYGPVYSIKTGASTVIVLNTAEVAKEAMVTRYSSISSRKLSKALTILTADKCMVAMSDYN 144
YGP+Y+I+TGAS+V+VLN+ EVAKEAMV ++SSIS+RKLSKALT+L+ DK MVA SD
Sbjct: 75 IYGPIYTIRTGASSVVVLNSTEVAKEAMVAKFSSISTRKLSKALTVLSHDKSMVATSDSG 134
Query: 145 DFHKLVKKYILANVLGANAQKRHRQRRDTMIGNISSQLFAHVKDTPSASVNFRNIFESEL 204
DFHK+ K+YI+ ++LG +AQK+ R RD +I N+ S VKD P A + FR++F+ EL
Sbjct: 135 DFHKMGKRYIMLSMLGTSAQKQFRDTRDMIINNMLSTFHQLVKDDPHAPLIFRDVFKDEL 194
Query: 205 FQLALKETLGKDIDSIYVDGLGTTLPREDLFRVLVLDPMEGAIEVDWRDFFPYLRWIPNK 264
F+L++ ++LG+D+ S+YVD G + +E+++ V D M AIEVDWRDFFPYL W+PNK
Sbjct: 195 FRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNATVTDMMMCAIEVDWRDFFPYLSWVPNK 254
Query: 265 SVEDKIKRMYFRRKMTMKSLIEEHKKRIVAGEETNCYADFLLSEAKTLTEDQITMLLWET 324
S E ++ RR M++LI++ K+RIV GE CY DFLL+E TLT++Q+ ML+WE
Sbjct: 255 SFETRVFTTESRRTAVMRALIKQQKERIVRGEARTCYLDFLLAE-NTLTDEQLMMLVWEA 314
Query: 325 IIETSDTTLVVTEWAMYELSKHTKRQDYLYKHIQRVCGSATLTEENLSQLPYLTAVFHET 384
+IE +DTTLV TEWAMYEL+K+ +Q+ LY+ I+ VCG +TEE+L LPYL AVF ET
Sbjct: 315 LIEAADTTLVTTEWAMYELAKNPDKQERLYQEIREVCGDEAVTEEHLPWLPYLNAVFQET 374
Query: 385 LRLHSPVPVVPLRYVQEDTQLGGFFVPAGSEIAVNIYACNMDEEHWESPEEWKPERFLDE 444
LR HSPVP++P R+V EDT L G+ VPAG+E+ +N+Y CNM+++ WESPEEW PERF
Sbjct: 375 LRRHSPVPLIPPRFVNEDTMLAGYDVPAGTEMVINLYGCNMNKKEWESPEEWAPERFAGG 434
Query: 445 KYDPMDLHKTMAFGGGRRVCAGALKAMLIACTTIGRLVQEFEWKLKGGEEDKVDTLGLTA 504
++ D++KTMAFG GRRVCAG+L+A IAC I R VQEF W+L+ G+E+KVDT+ LTA
Sbjct: 435 RFKVADMYKTMAFGAGRRVCAGSLQATHIACAAIARFVQEFGWRLREGDEEKVDTVQLTA 494
Query: 505 RKLQPLHAIIKPR 518
KL PLH + PR
Sbjct: 495 YKLHPLHVHLTPR 501
BLAST of Sed0020084 vs. ExPASy Swiss-Prot
Match:
Q0DBF4 (Ent-sandaracopimaradiene 3-hydroxylase OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A8 PE=1 SV=1)
HSP 1 Score: 523.1 bits (1346), Expect = 3.6e-147
Identity = 249/472 (52.75%), Postives = 349/472 (73.94%), Query Frame = 0
Query: 48 RRDLLPPVPAIPGVPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEV 107
R++ P PA+PG+P++GNL QLKEKKPH+TFA+W++TYGP+Y+IKTGAS V+VLN+ EV
Sbjct: 37 RKNRANPPPAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEV 96
Query: 108 AKEAMVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQKRH 167
AKEAM+ ++SSIS+RKL KA+++LT K MVA+SDY D+ K+ K+ I+ +LG NAQK+
Sbjct: 97 AKEAMIDKFSSISTRKLPKAMSVLTR-KSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQF 156
Query: 168 RQRRDTMIGNISSQLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVDGLGT 227
R R+ MI N+ S L V P + +NFR+++ +ELF L+L ++LG+D+ S+YV+ G
Sbjct: 157 RGTRERMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGR 216
Query: 228 TLPREDLFRVLVLDPMEGAIEVDWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKSLIEE 287
+ ++++F VLV + M A+E DWRD+FPYL W+PNKS + + FRR M +LI++
Sbjct: 217 EISKDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKK 276
Query: 288 HKKRIVAGEETNCYADFLLSEAKT--LTEDQITMLLWETIIETSDTTLVVTEWAMYELSK 347
K+RI GE Y DFLL ++ LT+DQ+ +LL E+I+ +DT LV TEW MYE++K
Sbjct: 277 QKERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIAK 336
Query: 348 HTKRQDYLYKHIQRVCGSATLTEENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQEDTQL 407
+ +Q+ LY+ I+ CG +TE++L +LPYL AVFHETLRLHSPVPV+P R+V +DT L
Sbjct: 337 NPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDDTTL 396
Query: 408 GGFFVPAGSEIAVNIYACNMDEEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGRRVCA 467
G+ + AG+++ +N+YAC+MDE+ WESP EW PERFL E ++ D +KTMAFG GRR CA
Sbjct: 397 AGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAGRRTCA 456
Query: 468 GALKAMLIACTTIGRLVQEFEWKLKGGEEDKVDTLGLTARKLQPLHAIIKPR 518
G+L+AM IAC + RLVQE EW+L+ G+ DK DT+ TA KL PLH +KPR
Sbjct: 457 GSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPR 507
BLAST of Sed0020084 vs. ExPASy TrEMBL
Match:
A0A5D3D9I5 (Ent-kaurene oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003760 PE=3 SV=1)
HSP 1 Score: 899.8 bits (2324), Expect = 5.3e-258
Identity = 442/519 (85.16%), Postives = 481/519 (92.68%), Query Frame = 0
Query: 1 MAIVTDPLGSVQNLVLALHAPPYTAAAVLGAVSFLVYVLLIGGWRKRRRDLLPPVPAIPG 60
MA+VTDPL S+Q L L APPY AAAVLG VS ++ V L+ RK+RR+ LPPVPA+PG
Sbjct: 1 MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAVPG 60
Query: 61 VPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEVAKEAMVTRYSSIS 120
VPLLGNLLQLKEKKPHKTFA+WA+TYGPVYSI+TGASTVIVLNT EVAKEAMVTRY SIS
Sbjct: 61 VPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120
Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQKRHRQRRDTMIGNISS 180
SRKLSKALTILTADKCMVAMSDYN+FHK+VK+YILANVLGANAQK+HRQRRD MI NIS
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180
Query: 181 QLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVDGLGTTLPREDLFRVLVL 240
+LFAHVK+ PS +VNFR IFESELF+LALKETLGKDI+SIYVDGLGTTLPREDLFR+LV+
Sbjct: 181 ELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240
Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKSLIEEHKKRIVAGEETNC 300
DPMEGAIEVDWRDFFPYLRWIPNKSVE+KI+ M FRR+MTMKSLI+E K+RI AGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTMKSLIQEPKRRIAAGEETNC 300
Query: 301 YADFLLSEAKTLTEDQITMLLWETIIETSDTTLVVTEWAMYELSKHTKRQDYLYKHIQRV 360
YADFLLSEAKTLTEDQI+MLLWETIIETSDTTLVVTEWAMYELSK KRQDYLY+ IQ V
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPKRQDYLYQQIQSV 360
Query: 361 CGSATLTEENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQEDTQLGGFFVPAGSEIAVNI 420
CGS+TLTEENLSQLPYLTA+FHETLR HSPVPVVPLRY EDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420
Query: 421 YACNMDEEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGRRVCAGALKAMLIACTTIGR 480
YACNMD+ HWE+PEEWKPERFLDEKYDPMDLHKTMAFGGG+RVCAGALKAMLIACTTIGR
Sbjct: 421 YACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480
Query: 481 LVQEFEWKLKGGEEDKVDTLGLTARKLQPLHAIIKPRIN 520
+VQEFEWKL+ GEE+KVDTLGLTARKLQPLH +IKPR N
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519
BLAST of Sed0020084 vs. ExPASy TrEMBL
Match:
A0A1S3BTZ0 (ent-kaurene oxidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493505 PE=3 SV=1)
HSP 1 Score: 899.8 bits (2324), Expect = 5.3e-258
Identity = 442/519 (85.16%), Postives = 481/519 (92.68%), Query Frame = 0
Query: 1 MAIVTDPLGSVQNLVLALHAPPYTAAAVLGAVSFLVYVLLIGGWRKRRRDLLPPVPAIPG 60
MA+VTDPL S+Q L L APPY AAAVLG VS ++ V L+ RK+RR+ LPPVPA+PG
Sbjct: 1 MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAVPG 60
Query: 61 VPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEVAKEAMVTRYSSIS 120
VPLLGNLLQLKEKKPHKTFA+WA+TYGPVYSI+TGASTVIVLNT EVAKEAMVTRY SIS
Sbjct: 61 VPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120
Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQKRHRQRRDTMIGNISS 180
SRKLSKALTILTADKCMVAMSDYN+FHK+VK+YILANVLGANAQK+HRQRRD MI NIS
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180
Query: 181 QLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVDGLGTTLPREDLFRVLVL 240
+LFAHVK+ PS +VNFR IFESELF+LALKETLGKDI+SIYVDGLGTTLPREDLFR+LV+
Sbjct: 181 ELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240
Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKSLIEEHKKRIVAGEETNC 300
DPMEGAIEVDWRDFFPYLRWIPNKSVE+KI+ M FRR+MTMKSLI+E K+RI AGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTMKSLIQEPKRRIAAGEETNC 300
Query: 301 YADFLLSEAKTLTEDQITMLLWETIIETSDTTLVVTEWAMYELSKHTKRQDYLYKHIQRV 360
YADFLLSEAKTLTEDQI+MLLWETIIETSDTTLVVTEWAMYELSK KRQDYLY+ IQ V
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPKRQDYLYQQIQSV 360
Query: 361 CGSATLTEENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQEDTQLGGFFVPAGSEIAVNI 420
CGS+TLTEENLSQLPYLTA+FHETLR HSPVPVVPLRY EDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420
Query: 421 YACNMDEEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGRRVCAGALKAMLIACTTIGR 480
YACNMD+ HWE+PEEWKPERFLDEKYDPMDLHKTMAFGGG+RVCAGALKAMLIACTTIGR
Sbjct: 421 YACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480
Query: 481 LVQEFEWKLKGGEEDKVDTLGLTARKLQPLHAIIKPRIN 520
+VQEFEWKL+ GEE+KVDTLGLTARKLQPLH +IKPR N
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519
BLAST of Sed0020084 vs. ExPASy TrEMBL
Match:
A0A6J1CGM6 (ent-kaurene oxidase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111010641 PE=3 SV=1)
HSP 1 Score: 896.0 bits (2314), Expect = 7.6e-257
Identity = 442/519 (85.16%), Postives = 475/519 (91.52%), Query Frame = 0
Query: 1 MAIVTDPLGSVQNLVLALHAPPYTAAAVLGAVSFLVYVLLIGGWRKRRRDLLPPVPAIPG 60
MAI +D L S+Q L+ +LHAPPY AAAVLG VS L+ V +IGGWRKR R+LLPPVPAIPG
Sbjct: 1 MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60
Query: 61 VPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEVAKEAMVTRYSSIS 120
+P+LGNLLQLKEKKPHKTFA+WA YGPVYSI+TGASTVIVLNTAEVAKEAMVTRYSSIS
Sbjct: 61 LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120
Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQKRHRQRRDTMIGNISS 180
SRKLSKALTILT+DKCMVAMSDYNDFHK+ K+YILANVLGANAQKRHRQRRDTMI NISS
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISS 180
Query: 181 QLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVDGLGTTLPREDLFRVLVL 240
LFAH KD+PS SVNFR IFE ELF+LALKETLG DI+SIYV LGTT REDLFR+LV+
Sbjct: 181 HLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIESIYVSELGTTALREDLFRILVI 240
Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKSLIEEHKKRIVAGEETNC 300
DPMEGAIEVDWRDFFPYLRWIPNK VEDKI+ M +RR++TMKSLIEE KKRI AGEETN
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNS 300
Query: 301 YADFLLSEAKTLTEDQITMLLWETIIETSDTTLVVTEWAMYELSKHTKRQDYLYKHIQRV 360
YA+FLLSEAKTLTEDQI+MLLWETIIETSDTTLVVTEWAMYEL+K KRQDYLY+HI V
Sbjct: 301 YAEFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELAKDPKRQDYLYQHILSV 360
Query: 361 CGSATLTEENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQEDTQLGGFFVPAGSEIAVNI 420
CGSA L EENL+QLPYLTAVFHETLR HSPVP+VPLRY EDTQLGGFFVPAGSEIAVNI
Sbjct: 361 CGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
Query: 421 YACNMDEEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGRRVCAGALKAMLIACTTIGR 480
Y CNMD+EHWESPEEWKPERFLDEKYDPMDLHKTMAFGGG+RVCAGALKA L+ACTTIGR
Sbjct: 421 YGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGR 480
Query: 481 LVQEFEWKLKGGEEDKVDTLGLTARKLQPLHAIIKPRIN 520
LVQEFEWKL GGEEDKVDTLGLTARKLQPLHAI+KPR N
Sbjct: 481 LVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN 519
BLAST of Sed0020084 vs. ExPASy TrEMBL
Match:
A0A6J1IL06 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476578 PE=3 SV=1)
HSP 1 Score: 895.2 bits (2312), Expect = 1.3e-256
Identity = 439/519 (84.59%), Postives = 482/519 (92.87%), Query Frame = 0
Query: 1 MAIVTDPLGSVQNLVLALHAPPYTAAAVLGAVSFLVYVLLIGGWRKRRRDLLPPVPAIPG 60
MAI DPLGS+Q+L+ L APPY AAA+LG +SFL+ L+ WRK RR+ LPPVPAIPG
Sbjct: 1 MAIFNDPLGSLQHLLDTLQAPPYAAAAILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60
Query: 61 VPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEVAKEAMVTRYSSIS 120
+P+LGNLLQLKEKKPHKTFA+WA+TYGP+YSI+TGASTVIVLNTAEVAKEAMVTRY+S+S
Sbjct: 61 LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120
Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQKRHRQRRDTMIGNISS 180
RKLSKALTILT+DKCMVAMSDYNDFHK+VKKYILANVLGANAQKRHRQRRD+MI NISS
Sbjct: 121 GRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDSMIDNISS 180
Query: 181 QLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVDGLGTTLPREDLFRVLVL 240
QLFAHVKD+PS SVNFR IFESELF LALKETLGKDI+SIYVDGLGTTLPREDLFRVLV+
Sbjct: 181 QLFAHVKDSPSESVNFRKIFESELFLLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240
Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKSLIEEHKKRIVAGEETNC 300
D MEGAIEVDWRDFFPYL+WIPNKSVE+KI++M +RR++TM SLIEE KKRI AGEETNC
Sbjct: 241 DLMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDYRRRVTMMSLIEEQKKRIAAGEETNC 300
Query: 301 YADFLLSEAKTLTEDQITMLLWETIIETSDTTLVVTEWAMYELSKHTKRQDYLYKHIQRV 360
YADFLLSEAKTLTE QI MLLWETIIETSDTTLVVTEWAMYEL+K+ +RQDYLY+HIQ V
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPERQDYLYQHIQSV 360
Query: 361 CGSATLTEENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQEDTQLGGFFVPAGSEIAVNI 420
CGS LTE++LS+LPYLTAVFHETLR HSPVP+VPLRYV ED QLGGFFVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420
Query: 421 YACNMDEEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGRRVCAGALKAMLIACTTIGR 480
YACNMD++ WESPEEWKPERFLDEKYDPMDLHKTMAFGGG+RVCAGALKAMLIACT+IGR
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480
Query: 481 LVQEFEWKLKGGEEDKVDTLGLTARKLQPLHAIIKPRIN 520
LVQEFEWKL GEEDKVDTLGLTARKLQPLH I+KPRI+
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHVIVKPRIS 519
BLAST of Sed0020084 vs. ExPASy TrEMBL
Match:
A0A6J1F7C8 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111442772 PE=3 SV=1)
HSP 1 Score: 891.3 bits (2302), Expect = 1.9e-255
Identity = 439/519 (84.59%), Postives = 482/519 (92.87%), Query Frame = 0
Query: 1 MAIVTDPLGSVQNLVLALHAPPYTAAAVLGAVSFLVYVLLIGGWRKRRRDLLPPVPAIPG 60
M I DPLGS+Q+L+ L AP Y AA++LG +SFL+ L+ WRK RR+ LPPVPAIPG
Sbjct: 1 MVIFNDPLGSLQHLLDTLQAPTYAAASILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60
Query: 61 VPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEVAKEAMVTRYSSIS 120
+P+LGNLLQLKEKKPHKTFA+WA+TYGP+YSI+TGASTVIVLNTAEVAKEAMVTRY+S+S
Sbjct: 61 LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120
Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQKRHRQRRDTMIGNISS 180
SRKLSKALTILT+DKCMVAMSDYNDFHK+VKKYILANVLGANAQKRHRQRRDTMI NISS
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDTMIDNISS 180
Query: 181 QLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVDGLGTTLPREDLFRVLVL 240
QLFAHVKD+PS SVNFR IFESELF+LALKETLGKDI+SIYVDGLG LPREDLFRVLV+
Sbjct: 181 QLFAHVKDSPSESVNFRRIFESELFRLALKETLGKDIESIYVDGLG-MLPREDLFRVLVI 240
Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKSLIEEHKKRIVAGEETNC 300
DPMEGAIEVDWRDFFPYL+WIPNKSVE+KI++M FRR++TM SLIE+ KKRI AGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDFRRRVTMMSLIEKQKKRIAAGEETNC 300
Query: 301 YADFLLSEAKTLTEDQITMLLWETIIETSDTTLVVTEWAMYELSKHTKRQDYLYKHIQRV 360
YADFLLSEAKTLTE QI MLLWETIIETSDTTLVVTEWAMYEL+K+ KRQDYLY+HIQ V
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPKRQDYLYQHIQSV 360
Query: 361 CGSATLTEENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQEDTQLGGFFVPAGSEIAVNI 420
CGS LTE++LS+LPYLTAVFHETLR HSPVP+VPLRYV ED QLGGFFVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420
Query: 421 YACNMDEEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGRRVCAGALKAMLIACTTIGR 480
YACNMD++ WESPEEWKPERFLDEKYDPMDLHKTMAFGGG+RVCAGALKAMLIACT+IGR
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480
Query: 481 LVQEFEWKLKGGEEDKVDTLGLTARKLQPLHAIIKPRIN 520
LVQEFEWKL GEEDKVDTLGLTARKLQPLHAI+KPRI+
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHAIVKPRIS 518
BLAST of Sed0020084 vs. TAIR 10
Match:
AT5G25900.1 (GA requiring 3 )
HSP 1 Score: 624.0 bits (1608), Expect = 1.1e-178
Identity = 302/467 (64.67%), Postives = 374/467 (80.09%), Query Frame = 0
Query: 52 LPPVPAIPGVPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEVAKEA 111
LP VP +PG P++GNLLQLKEKKPHKTF +W++ YGP+YSIK G+S++IVLN+ E AKEA
Sbjct: 41 LPSVPVVPGFPVIGNLLQLKEKKPHKTFTRWSEIYGPIYSIKMGSSSLIVLNSTETAKEA 100
Query: 112 MVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQKRHRQRR 171
MVTR+SSIS+RKLS ALT+LT DK MVA SDY+DFHKLVK+ +L +LGANAQKR R R
Sbjct: 101 MVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYR 160
Query: 172 DTMIGNISSQLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVDGLGTTLPR 231
D +I N+SS+L AH +D P VNFR IFE ELF +ALK+ GKD++SIYV LG TL +
Sbjct: 161 DALIENVSSKLHAHARDHPQEPVNFRAIFEHELFGVALKQAFGKDVESIYVKELGVTLSK 220
Query: 232 EDLFRVLVLDPMEGAIEVDWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKSLIEEHKKR 291
+++F+VLV D MEGAI+VDWRDFFPYL+WIPNKS E +I++ + RR M +LI++ K+
Sbjct: 221 DEIFKVLVHDMMEGAIDVDWRDFFPYLKWIPNKSFEARIQQKHKRRLAVMNALIQDRLKQ 280
Query: 292 IVAGEETNCYADFLLSEAKTLTEDQITMLLWETIIETSDTTLVVTEWAMYELSKHTKRQD 351
+ + +CY +FL+SEAKTLT++QI +L+WETIIET+DTTLV TEWA+YEL+KH QD
Sbjct: 281 NGSESDDDCYLNFLMSEAKTLTKEQIAILVWETIIETADTTLVTTEWAIYELAKHPSVQD 340
Query: 352 YLYKHIQRVCGSATLTEENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQEDTQLGGFFVP 411
L K IQ VCG EE LSQ+PYL VFHETLR +SP P+VP+RY EDTQ+GG+ VP
Sbjct: 341 RLCKEIQNVCGGEKFKEEQLSQVPYLNGVFHETLRKYSPAPLVPIRYAHEDTQIGGYHVP 400
Query: 412 AGSEIAVNIYACNMDEEHWESPEEWKPERFLDE-KYDPMDLHKTMAFGGGRRVCAGALKA 471
AGSEIA+NIY CNMD++ WE PE+W PERFLD+ KY+ DLHKTMAFG G+RVCAGAL+A
Sbjct: 401 AGSEIAINIYGCNMDKKRWERPEDWWPERFLDDGKYETSDLHKTMAFGAGKRVCAGALQA 460
Query: 472 MLIACTTIGRLVQEFEWKLKGGEEDKVDTLGLTARKLQPLHAIIKPR 518
L+A IGRLVQEFEWKL+ GEE+ VDT GLT++KL PL AII PR
Sbjct: 461 SLMAGIAIGRLVQEFEWKLRDGEEENVDTYGLTSQKLYPLMAIINPR 507
BLAST of Sed0020084 vs. TAIR 10
Match:
AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )
HSP 1 Score: 187.2 bits (474), Expect = 3.4e-47
Identity = 148/510 (29.02%), Postives = 243/510 (47.65%), Query Frame = 0
Query: 34 FLVYVLLIGGWRKRRRDLLPPVPAIPGVPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIK 93
FL++ G R LPP P P +P++GN + L K PH++FA+ +KTYGPV S+K
Sbjct: 20 FLIFTTTRSGRISRGATALPPGP--PRLPIIGN-IHLVGKHPHRSFAELSKTYGPVMSLK 79
Query: 94 TGASTVIVLNTAEVAKEAMVTRYSSISSRKLSKAL-TILTADKCMVAMSDYNDFHKLVKK 153
G+ +V+ + E A+E + T +S+R + A+ +I D +V + + +L+++
Sbjct: 80 LGSLNTVVIASPEAAREVLRTHDQILSARSPTNAVRSINHQDASLVWLPSSSARWRLLRR 139
Query: 154 YILANVLGANAQKRHRQRRDTMIGNI------SSQLFAHVKDTPSASVNFRNIFESELFQ 213
+ +L + + R + + SS V + A + NI + LF
Sbjct: 140 LSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAFITTLNIISNILFS 199
Query: 214 LALKETLGKDIDSIYVDGLGTTLPREDLFRVLVLDPMEGAIEVDWRDFFPYLRWI----- 273
+ LG ++G+ T V+ M+ A D ++FP+LR++
Sbjct: 200 V----DLGSYNAKASINGVQDT----------VISVMDAAGTPDAANYFPFLRFLDLQGN 259
Query: 274 --PNKSVEDKIKRMYFRRKMTMKSLIEEHKKRIVAGEETNCYADFLL---SEAKTLTEDQ 333
K +++ R++ R + E+ + N + D LL + L+
Sbjct: 260 VKTFKVCTERLVRVF--RGFIDAKIAEKSSQNNPKDVSKNDFVDNLLDYKGDESELSISD 319
Query: 334 ITMLLWETIIETSDTTLVVTEWAMYELSKHTKRQDYLYKHIQRVCGSATLTEE-NLSQLP 393
I LL + +DT+ EWAM EL K+ K I V G + EE ++S+LP
Sbjct: 320 IEHLLLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQNGIVEESDISKLP 379
Query: 394 YLTAVFHETLRLHSPVPVVPLRYVQEDTQLGGFFVPAGSEIAVNIYACNMDEEHWESPEE 453
YL AV ET RLH+PVP++ R + D ++ GF V +++ VN++A D W++P +
Sbjct: 380 YLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNPSQ 439
Query: 454 WKPERFLDEKYDPMDL-HKTMAFGGGRRVCAGALKAMLIACTTIGRLVQEFEWKLKGG-- 513
++PERFL + D ++ FG GRR+C G AM + L+ F+WKL G
Sbjct: 440 FEPERFLGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKLPKGVL 499
Query: 514 --EEDKVDTLGLTARKLQPLHAI-IKPRIN 520
+ D +T GLT K PLHA+ +K R N
Sbjct: 500 SEDLDMDETFGLTLHKTNPLHAVPVKKRAN 510
BLAST of Sed0020084 vs. TAIR 10
Match:
AT2G40890.1 (cytochrome P450, family 98, subfamily A, polypeptide 3 )
HSP 1 Score: 183.0 bits (463), Expect = 6.3e-46
Identity = 129/485 (26.60%), Postives = 233/485 (48.04%), Query Frame = 0
Query: 46 KRRRDLLPPVPAIPGVPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTA 105
+R R PP P+ P++GNL +K + + + +WA++YGP+ S+ G+ +V+++A
Sbjct: 21 QRLRYKFPPGPS--PKPIVGNLYDIKPVR-FRCYYEWAQSYGPIISVWIGSILNVVVSSA 80
Query: 106 EVAKEAMVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQK 165
E+AKE + ++ R +++ + + + +DY + V+K + +
Sbjct: 81 ELAKEVLKEHDQKLADRHRNRSTEAFSRNGQDLIWADYGPHYVKVRKVCTLELFTPKRLE 140
Query: 166 RHRQRRDTMIGNISSQLF--AHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVD 225
R R+ + + +F ++ + + + R + F + GK +++
Sbjct: 141 SLRPIREDEVTAMVESVFRDCNLPENRAKGLQLRKYLGAVAFNNITRLAFGKR----FMN 200
Query: 226 GLGTTLPREDLFRVLVLDPMEGAIEVDWRDFFPYLRWIPNKSVEDKIKRMYFRRKMTMKS 285
G + F+ +V + ++ + + P+LRW+ + E RR ++
Sbjct: 201 AEGVVDEQGLEFKAIVSNGLKLGASLSIAEHIPWLRWM-FPADEKAFAEHGARRDRLTRA 260
Query: 286 LIEEH---KKRIVAGEETNCYADFLLSEAKTLTEDQITMLLWETIIETSDTTLVVTEWAM 345
++EEH +++ ++ A L + L+ED I LLW+ I DTT + EWAM
Sbjct: 261 IMEEHTLARQKSSGAKQHFVDALLTLKDQYDLSEDTIIGLLWDMITAGMDTTAITAEWAM 320
Query: 346 YELSKHTKRQDYLYKHIQRVCG-SATLTEENLSQLPYLTAVFHETLRLHSPVPVVPLRYV 405
E+ K+ + Q + + RV G LTE + S+LPYL V E+ RLH P P++
Sbjct: 321 AEMIKNPRVQQKVQEEFDRVVGLDRILTEADFSRLPYLQCVVKESFRLHPPTPLMLPHRS 380
Query: 406 QEDTQLGGFFVPAGSEIAVNIYACNMDEEHWESPEEWKPERFLDEKYDPMDLH--KTMAF 465
D ++GG+ +P GS + VN++A D W++P E++PERFL+E D M H + + F
Sbjct: 381 NADVKIGGYDIPKGSNVHVNVWAVARDPAVWKNPFEFRPERFLEEDVD-MKGHDFRLLPF 440
Query: 466 GGGRRVCAGALKAMLIACTTIGRLVQEFEWK----LKGGEEDKVDTLGLTARKLQPLHAI 519
G GRRVC GA + + + + L+ F W K E D + GL P+ A+
Sbjct: 441 GAGRRVCPGAQLGINLVTSMMSHLLHHFVWTPPQGTKPEEIDMSENPGLVTYMRTPVQAV 496
BLAST of Sed0020084 vs. TAIR 10
Match:
AT1G74550.1 (cytochrome P450, family 98, subfamily A, polypeptide 9 )
HSP 1 Score: 177.9 bits (450), Expect = 2.0e-44
Identity = 129/481 (26.82%), Postives = 230/481 (47.82%), Query Frame = 0
Query: 46 KRRRDLLPPVPAIPGVPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTA 105
+RR +PP P P L+GNL QLK ++F++W++TYGP+ S+ G+ +V++++
Sbjct: 21 RRRGSNIPPGP--PTRFLVGNLHQLKPLWT-QSFSEWSQTYGPIISVWLGSQLAVVVSSS 80
Query: 106 EVAKEAMVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKKYILANVLGANAQK 165
++AK+ + + + +R + +T +D + SDY + ++K + + +
Sbjct: 81 DLAKQVLRDKDYQLCNRHRTARMTQNGSD---LIWSDYGAHYVKMRKLCTLELFSLKSIE 140
Query: 166 RHRQRRDTMIGNISSQLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIYVDGL 225
R R+ + ++ +F V RN +S + + +GK + DG
Sbjct: 141 CFRSMREMEVSSMVKSIFNDFMSDDQKPVVLRNYLDSVALNIVSRLVIGKTFEP--KDG- 200
Query: 226 GTTLPREDLFRVLVLDPMEGAIEVDWRDFFPYLRWIPNKSVEDK--IKRMYFRRKMTMKS 285
RE FR +V D+ +L+ + + DK +K M +R ++
Sbjct: 201 -----RE--FRSIVERETRLPGATKMLDYTVWLKRLSSWFTSDKAFMKHMARKRNWFKRA 260
Query: 286 LIEEHKKRIVAGEETNCYADFL--LSEAKTLTEDQITMLLWETIIETSDTTLVVTEWAMY 345
+++E + G + C+ L L E LTE+ + L+W + +DTT + EWAM
Sbjct: 261 VMDE----VYGGRDQKCFVQSLLELKEKDELTEETVMGLVWNMLTAGADTTAITIEWAMA 320
Query: 346 ELSKHTKRQDYLYKHIQRVCGSATL-TEENLSQLPYLTAVFHETLRLHSPVPVVPLRYVQ 405
E+ + ++ + + V GS L ++ ++ +LP+L V E LRLH P P++
Sbjct: 321 EMIRCPTVKEKVQDELDSVVGSGRLMSDADIPKLPFLQCVLKEALRLHPPTPLMLPHKAS 380
Query: 406 EDTQLGGFFVPAGSEIAVNIYACNMDEEHWESPEEWKPERFLDEKYDPMDL-HKTMAFGG 465
E Q+GG+ VP G+ + VN+ A D +W +P+E++PERFL E+ D + + FG
Sbjct: 381 ESVQVGGYKVPKGATVYVNVQAIARDPANWSNPDEFRPERFLVEETDVKGQDFRVLPFGS 440
Query: 466 GRRVCAGALKAMLIACTTIGRLVQEFEWKLKGGEE--DKVDTLGLTARKLQPLHAIIKPR 519
GRRVC A ++ + +G L+ F W E D + GL PL A+ R
Sbjct: 441 GRRVCPAAQLSLNMMTLALGSLLHCFSWTSSTPREHIDMTEKPGLVCYMKAPLQALASSR 481
BLAST of Sed0020084 vs. TAIR 10
Match:
AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )
HSP 1 Score: 177.6 bits (449), Expect = 2.7e-44
Identity = 135/488 (27.66%), Postives = 227/488 (46.52%), Query Frame = 0
Query: 49 RDLLPPVPAIPGVPLLGNLLQLKEKKPHKTFAQWAKTYGPVYSIKTGASTVIVLNTAEVA 108
R ++P P P +P++GN + L + PH +FA +KTYGP+ S+K G+ +V+ + E A
Sbjct: 33 RKVVPSPPGPPRLPIIGN-IHLVGRNPHHSFADLSKTYGPIMSLKFGSLNTVVVTSPEAA 92
Query: 109 KEAMVTRYSSISSRKLSKALTILTADKCMVA-MSDYNDFHKLVKKYILANVLGANAQKRH 168
+E + T +SSR + ++ + DK V + + +L++K + +
Sbjct: 93 REVLRTYDQILSSRTPTNSIRSINHDKVSVVWLPPSSSRWRLLRKLSATQLFSPQRIEAT 152
Query: 169 RQRRDTMIGNI------SSQLFAHVKDTPSASVNFRNIFESELFQLALKETLGKDIDSIY 228
+ R+ + + SS+ V + + + NI + LF + L + DS
Sbjct: 153 KTLRENKVKELVSFMSESSEREEAVDISRATFITALNIISNILFSVDL-----GNYDS-- 212
Query: 229 VDGLGTTLPREDLFRVLVLDPMEGAIEVDWRDFFPYLRWIP----NKSVED------KIK 288
+ +F+ V+ ME D +FFP+L ++ K+++ K+
Sbjct: 213 --------NKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLKACSERLFKVF 272
Query: 289 RMYFRRKMTMKSLIEEHKKRIVAGEETNCYADFLLSEAKTLTEDQITMLLWETIIETSDT 348
R + K+ KSL + + K + + + D + L + I LL + +DT
Sbjct: 273 RGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTNDIVHLLLDLFGAGTDT 332
Query: 349 TLVVTEWAMYELSKHTKRQDYLYKHIQRVCGSATLTEE-NLSQLPYLTAVFHETLRLHSP 408
EWAM EL ++ + I V G + EE ++S LPYL AV ET RLH
Sbjct: 333 NSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYLQAVVKETFRLHPA 392
Query: 409 VPVVPLRYVQEDTQLGGFFVPAGSEIAVNIYACNMDEEHWESPEEWKPERFLDEKYDPMD 468
P++ R + D ++ GF VP +++ VN++A D WE+ +KPERFL + D
Sbjct: 393 APLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPERFLGKDIDLRG 452
Query: 469 L-HKTMAFGGGRRVCAGALKAMLIACTTIGRLVQEFEWKLKGG----EEDKVDTLGLTAR 514
++ FG GRR+C G A+ + L+ F+WKL G + D +T GLT
Sbjct: 453 RDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDLDMDETFGLTLH 504
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038898390.1 | 4.9e-258 | 85.36 | ent-kaurene oxidase [Benincasa hispida] | [more] |
XP_008452488.1 | 1.1e-257 | 85.16 | PREDICTED: ent-kaurene oxidase, chloroplastic [Cucumis melo] >KAA0064350.1 ent-k... | [more] |
XP_022139822.1 | 1.6e-256 | 85.16 | ent-kaurene oxidase, chloroplastic [Momordica charantia] >XP_022139833.1 ent-kau... | [more] |
XP_022976063.1 | 2.7e-256 | 84.59 | ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima] | [more] |
XP_023536271.1 | 3.5e-256 | 84.59 | ent-kaurene oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
A0A0G2RKY1 | 5.7e-185 | 62.35 | Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1 | [more] |
Q93ZB2 | 1.5e-177 | 64.67 | Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV... | [more] |
Q5Z5R4 | 4.4e-161 | 55.98 | Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 ... | [more] |
Q5Z5R7 | 7.0e-159 | 55.38 | Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19... | [more] |
Q0DBF4 | 3.6e-147 | 52.75 | Ent-sandaracopimaradiene 3-hydroxylase OS=Oryza sativa subsp. japonica OX=39947 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A5D3D9I5 | 5.3e-258 | 85.16 | Ent-kaurene oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G... | [more] |
A0A1S3BTZ0 | 5.3e-258 | 85.16 | ent-kaurene oxidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493505 PE=3 ... | [more] |
A0A6J1CGM6 | 7.6e-257 | 85.16 | ent-kaurene oxidase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11101064... | [more] |
A0A6J1IL06 | 1.3e-256 | 84.59 | ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476... | [more] |
A0A6J1F7C8 | 1.9e-255 | 84.59 | ent-kaurene oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1114... | [more] |
Match Name | E-value | Identity | Description | |
AT5G25900.1 | 1.1e-178 | 64.67 | GA requiring 3 | [more] |
AT2G45560.1 | 3.4e-47 | 29.02 | cytochrome P450, family 76, subfamily C, polypeptide 1 | [more] |
AT2G40890.1 | 6.3e-46 | 26.60 | cytochrome P450, family 98, subfamily A, polypeptide 3 | [more] |
AT1G74550.1 | 2.0e-44 | 26.82 | cytochrome P450, family 98, subfamily A, polypeptide 9 | [more] |
AT2G45570.1 | 2.7e-44 | 27.66 | cytochrome P450, family 76, subfamily C, polypeptide 2 | [more] |