Sed0019957 (gene) Chayote v1

Overview
NameSed0019957
Typegene
OrganismSechium edule (Chayote v1)
DescriptionRNA-directed DNA polymerase (Reverse transcriptase)
LocationLG01: 62509002 .. 62509895 (+)
RNA-Seq ExpressionSed0019957
SyntenySed0019957
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGATTTCTCGGTTAGAAGTGCTCACTGGCTGTTTAGACATAATTTTTTTGAAGCCTCTGGGGAAAAAGTGTCTTTGGAACCTTAAAGTTCCAAATAAGTTGAAGCTTTTCATGTGGAGACTCTATCATGAATTTCTACCAACCAACCTTGTGTTACAAAAAAGAAAGGTTCCTGTTCAGCCTTGGTGTGTGATGTGTGGAAATTTTTATGAATCATTTTTCCATATATTTTTTGAATGCCCCTGTGCCAATGCTTTGTGGAAAGCTTTGGATCTTAGTTTTCATCCAGAATCTCTGAAGTTGTTGGATACTCAAAGTGCCATTGAATTTTTAGGAGCCGGTCTTTCGAAAGACAATCTGGCTTATGCAGTTATTTGCCTCTGGTCAATTTGGATCGATCGAAATAAAGTAAATTTTAATGAGGTTGTCCCCCCAGTTAATGTTCGAATTGAATGGATTCGTCAGTATTTGGAGGGTGTTTTGAAATCTAATGATATGGAATCCCATAAGCATGTTGAGTATGATTCTTGCTCTACTGTTGCTCCTGTTCGTTGGATTGCGCCATGGCCGGGATTTCTCAAGCTTAATGTCGATGCTTCATGTTCCCCTTTAGCGCCGAAATTGGGGTTTGGATTAATTTTCAGAGATCACATGGGATTATGCAAATTTGCTTCTTCCATTTTCAAACCTGTGTTTTGTGATATCCTCTCAGCGGAAGCTTTGGCTTTGTTAGAGGGATTGAAGGTGGCTGATAATCTTGGATTCCATAATTTGATTATTGAATCTGATTCAAAGACACTTGTCGATGCTATTCTAGGGAATCGTTTATCTCTTTCTCCTCAAGGTATTATCCTTGATGAAATTCGTCTGCTGCTAAAGAAATATGGCTAA

mRNA sequence

ATGGAGATTTCTCGGTTAGAAGTGCTCACTGGCTGTTTAGACATAATTTTTTTGAAGCCTCTGGGGAAAAAGTGTCTTTGGAACCTTAAAGTTCCAAATAAGTTGAAGCTTTTCATGTGGAGACTCTATCATGAATTTCTACCAACCAACCTTGTGTTACAAAAAAGAAAGGTTCCTGTTCAGCCTTGGTGTGTGATGTGTGGAAATTTTTATGAATCATTTTTCCATATATTTTTTGAATGCCCCTGTGCCAATGCTTTGTGGAAAGCTTTGGATCTTAGTTTTCATCCAGAATCTCTGAAGTTGTTGGATACTCAAAGTGCCATTGAATTTTTAGGAGCCGGTCTTTCGAAAGACAATCTGGCTTATGCAGTTATTTGCCTCTGGTCAATTTGGATCGATCGAAATAAAGTAAATTTTAATGAGGTTGTCCCCCCAGTTAATGTTCGAATTGAATGGATTCGTCAGTATTTGGAGGGTGTTTTGAAATCTAATGATATGGAATCCCATAAGCATGTTGAGTATGATTCTTGCTCTACTGTTGCTCCTGTTCGTTGGATTGCGCCATGGCCGGGATTTCTCAAGCTTAATGTCGATGCTTCATGTTCCCCTTTAGCGCCGAAATTGGGGTTTGGATTAATTTTCAGAGATCACATGGGATTATGCAAATTTGCTTCTTCCATTTTCAAACCTGTGTTTTGTGATATCCTCTCAGCGGAAGCTTTGGCTTTGTTAGAGGGATTGAAGGTGGCTGATAATCTTGGATTCCATAATTTGATTATTGAATCTGATTCAAAGACACTTGTCGATGCTATTCTAGGGAATCGTTTATCTCTTTCTCCTCAAGGTATTATCCTTGATGAAATTCGTCTGCTGCTAAAGAAATATGGCTAA

Coding sequence (CDS)

ATGGAGATTTCTCGGTTAGAAGTGCTCACTGGCTGTTTAGACATAATTTTTTTGAAGCCTCTGGGGAAAAAGTGTCTTTGGAACCTTAAAGTTCCAAATAAGTTGAAGCTTTTCATGTGGAGACTCTATCATGAATTTCTACCAACCAACCTTGTGTTACAAAAAAGAAAGGTTCCTGTTCAGCCTTGGTGTGTGATGTGTGGAAATTTTTATGAATCATTTTTCCATATATTTTTTGAATGCCCCTGTGCCAATGCTTTGTGGAAAGCTTTGGATCTTAGTTTTCATCCAGAATCTCTGAAGTTGTTGGATACTCAAAGTGCCATTGAATTTTTAGGAGCCGGTCTTTCGAAAGACAATCTGGCTTATGCAGTTATTTGCCTCTGGTCAATTTGGATCGATCGAAATAAAGTAAATTTTAATGAGGTTGTCCCCCCAGTTAATGTTCGAATTGAATGGATTCGTCAGTATTTGGAGGGTGTTTTGAAATCTAATGATATGGAATCCCATAAGCATGTTGAGTATGATTCTTGCTCTACTGTTGCTCCTGTTCGTTGGATTGCGCCATGGCCGGGATTTCTCAAGCTTAATGTCGATGCTTCATGTTCCCCTTTAGCGCCGAAATTGGGGTTTGGATTAATTTTCAGAGATCACATGGGATTATGCAAATTTGCTTCTTCCATTTTCAAACCTGTGTTTTGTGATATCCTCTCAGCGGAAGCTTTGGCTTTGTTAGAGGGATTGAAGGTGGCTGATAATCTTGGATTCCATAATTTGATTATTGAATCTGATTCAAAGACACTTGTCGATGCTATTCTAGGGAATCGTTTATCTCTTTCTCCTCAAGGTATTATCCTTGATGAAATTCGTCTGCTGCTAAAGAAATATGGCTAA

Protein sequence

MEISRLEVLTGCLDIIFLKPLGKKCLWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIFFECPCANALWKALDLSFHPESLKLLDTQSAIEFLGAGLSKDNLAYAVICLWSIWIDRNKVNFNEVVPPVNVRIEWIRQYLEGVLKSNDMESHKHVEYDSCSTVAPVRWIAPWPGFLKLNVDASCSPLAPKLGFGLIFRDHMGLCKFASSIFKPVFCDILSAEALALLEGLKVADNLGFHNLIIESDSKTLVDAILGNRLSLSPQGIILDEIRLLLKKYG
Homology
BLAST of Sed0019957 vs. NCBI nr
Match: MCH79484.1 (RNA-directed DNA polymerase (Reverse transcriptase) [Trifolium medium])

HSP 1 Score: 144.1 bits (362), Expect = 2.0e-30
Identity = 97/286 (33.92%), Postives = 136/286 (47.55%), Query Frame = 0

Query: 12  CLDIIFLKPLGKKCLWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFY 71
           C+D+  L P  K  LWNLK+P ++  FMWRL H+ LPT + L  R +P    CV C    
Sbjct: 184 CVDL--LHPHWKN-LWNLKIPPRVSSFMWRLAHQCLPTRVNLTTRGIPCDDTCVSCETLA 243

Query: 72  ESFFHIFFECPCANALWKALDLSFHPESLKLLD---TQSAIEFLGAGLSKDNLAYAVICL 131
           ES  HIFF C  A   W  +DL  H   L L     T    +FL   LS      A + L
Sbjct: 244 ESHMHIFFVCSKAMECWGRIDLGHHVRELLLRTSEFTTLLFDFLDR-LSFIQQQLAAMLL 303

Query: 132 WSIWIDRN-KVNFNEVVPPVNVRIEWIRQYLEGVLKSNDMESHKHVEYDSCSTVAPVRWI 191
           WSIW  R+ K+  +    P  +         E           +H+ + +        W+
Sbjct: 304 WSIWKSRDLKLWESSSTSPTLIVTRAKETLHEWSCMQRIKPQVQHLAHTN-------TWV 363

Query: 192 APWPGFLKLNVDASCSPLAPKLGFGLIFRDHMGLCKFASSIFKPVFCDILSAEALALLEG 251
            P  G +K NVD +        GFG+ FRD +G   F  S   P F  +L AEA+ALL+ 
Sbjct: 364 KPTIGVIKCNVDVTTFNNNTMAGFGMCFRDSLGQFIFGKSAHLPSFGFVLEAEAVALLQS 423

Query: 252 LKVADNLGFHNLIIESDSKTLVDAILGNRLSLSPQGIILDEIRLLL 294
           +  A ++G H++I E+D+KTL +AI       +  G I+ + R+LL
Sbjct: 424 IHTAISIGLHSVIFETDNKTLANAINSTSAHNNEFGDIVTQCRVLL 458

BLAST of Sed0019957 vs. NCBI nr
Match: XP_031094430.1 (uncharacterized protein LOC115998901 [Ipomoea triloba])

HSP 1 Score: 137.9 bits (346), Expect = 1.4e-28
Identity = 85/277 (30.69%), Postives = 141/277 (50.90%), Query Frame = 0

Query: 19  KPLGKKCLWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIF 78
           + LG   +W L +P K K F W L    LPT  +L  ++V     C++CG   ES  H+F
Sbjct: 51  RELGWTGVWYLNLPPKAKCFFWTLCSMCLPTKDLLYMKRVNCDQVCMLCGLANESVVHLF 110

Query: 79  FECPCANALWKALDLSFHPESLKLLDTQSAIEFLGAGLSKDNLAYAVICLWSIWIDRNKV 138
             C  A   W+ LD ++   +L  +D+QS +  +   LSK+ L   V+  W++W +RN +
Sbjct: 111 ANCSFARDCWRELDAAW---NLSYVDSQSWLAEMWNVLSKNMLEKVVMVCWALWENRNSL 170

Query: 139 NF-NEVVPPVNVRIEWIRQYLEGVLKSNDMESHKHVEYDSCSTVAPVRWIAPWPGFLKLN 198
            + N  +PP+ V ++    + E   K   + +H+     + + +    W+ P PG+LKLN
Sbjct: 171 CWKNTGMPPITV-VQHALAFYEN-WKKVTVATHESALISNTAVLDSTSWLPPPPGYLKLN 230

Query: 199 VDASCSPLAPKLGFGLIFRDHMGLCKFASSIFKPVFCDILSAEALALLEGLKVADNLGFH 258
           +D S +    ++GFG + RD  G+              +  AEA+   E L     +G+ 
Sbjct: 231 IDVSMAVHHRRMGFGWVCRDETGIVVGVRMNSMMGLYSVREAEAMGAREVLSWIKLMGWE 290

Query: 259 NLIIESDSKTLVDAILGNRLSLSPQGIILDEIRLLLK 295
            +I+E+D++ +  A+ G  L  SP G I+ +IRLLLK
Sbjct: 291 RVILETDAQIVTRAVQGG-LDFSPFGTIIHDIRLLLK 321

BLAST of Sed0019957 vs. NCBI nr
Match: KAF4382082.1 (hypothetical protein F8388_001227 [Cannabis sativa])

HSP 1 Score: 137.1 bits (344), Expect = 2.4e-28
Identity = 82/273 (30.04%), Postives = 132/273 (48.35%), Query Frame = 0

Query: 24  KCLWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIFFECPC 83
           K +W  ++  K+K+F+WR++H ++P    L KR + +   C  C +  E   H  +  P 
Sbjct: 444 KVVWGSRLTPKMKIFIWRVFHHWIPVKTELTKRGMSMNLTCNRCKSQVEDVCHALWNIPK 503

Query: 84  ANALWKALDLSFH-PESLKLLDTQSAIEFLGAGLSKDNLAYAVICLWSIWIDRNKVNFNE 143
            + +WK      H P SLK      A +FL     K +  + +   W IW  RNK  F  
Sbjct: 504 LHKVWKHFGFLHHFPSSLK-----QAPDFLMLMKGKLSEEFFIGITWLIWYQRNKCVFQN 563

Query: 144 VVPPVNVRIEWIRQYLEGVLKSNDMESHKHVEYDSCSTVAPVRWIAPWPGFLKLNVDASC 203
                ++ I W  + +E  L S    SH+++  D+      V+W  P PG   +N DAS 
Sbjct: 564 KDIEDDIWIPWATEMMEIHLASAQTNSHQNISKDT------VKWSPPPPGTFMINTDASL 623

Query: 204 SPLAPKLGFGLIFRDHMGLCKFASSIFKPVFCDILSAEALALLEGLKVADNLGFHNLIIE 263
               P  G G+I RDH+G    A++ F P    +L AEA+A+   LK+A+     N+ I 
Sbjct: 624 IEGQPGCGLGVIIRDHLGALVTATTEFIPGCLSVLLAEAMAIRLALKLAETRLMQNIFIA 683

Query: 264 SDSKTLVDAILGNRLSLSPQGIILDEIRLLLKK 296
           SDS+++++A+ G     +  G ++D+     KK
Sbjct: 684 SDSQSVINALKGKTHINTDWGFVIDDCIQASKK 705

BLAST of Sed0019957 vs. NCBI nr
Match: XP_019163602.1 (PREDICTED: uncharacterized protein LOC109159944 [Ipomoea nil])

HSP 1 Score: 132.9 bits (333), Expect = 4.6e-27
Identity = 87/283 (30.74%), Postives = 139/283 (49.12%), Query Frame = 0

Query: 26   LWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIFFECPCAN 85
            +W LK+P+++  F+W + H  + TNL   +RK+     C  C    E   H+   CP A 
Sbjct: 1278 IWKLKIPSRICFFLWLVRHNKIMTNLCRTQRKLTNDDSCWTCVGTPEDTNHVLRFCPAAE 1337

Query: 86   ALWKALDLSFHPESLKL-----LDTQSAIEFLGAGLSKDNLAYAVICLWSIWIDRNKVNF 145
            A+W A+   FH ++  L     LD   A +  G     DN+ +AV  LW +W  RN+  F
Sbjct: 1338 AIWTAILPEFHDKTKHLPLVPWLDKGVAAKGNGRRPVSDNITFAV-TLWWVWKWRNEAIF 1397

Query: 146  NEVVPPVNVRIEWIRQYLEGVLKSNDMESHKHVEYDSCSTVAP--------VRWIAPWPG 205
              +V P++V+++ IR ++E +  S          +  C    P        + W  P PG
Sbjct: 1398 KNLVKPLSVKVQQIRTHIEDINNS----------FARCMNPIPTVDQSWKMLTWKKPPPG 1457

Query: 206  FLKLNVDASCSPLAPKLGFGLIFRDHMGLCKFASSIFKPVFCDILSAEALALLEGLKVAD 265
             LKLN+D S +PL+   G G + R+  G       I K   C  L AEA ++L+G++ A 
Sbjct: 1458 TLKLNIDGSVAPLSLTAGCGGVIRNSSGEW-ITGFIAKLGTCTPLEAEAWSILKGIQFAI 1517

Query: 266  NLGFHNLIIESDSKTLVDAILGNRLSLSPQGIILDEIRLLLKK 296
              G+ N++IESDS  +V+ +  +R S      IL+  +  L++
Sbjct: 1518 AKGYSNVLIESDSSDVVNFLQDSRPSCIAAHNILEACKKELRR 1548

BLAST of Sed0019957 vs. NCBI nr
Match: XP_024190234.1 (uncharacterized protein LOC112194221 [Rosa chinensis])

HSP 1 Score: 132.1 bits (331), Expect = 7.9e-27
Identity = 88/279 (31.54%), Postives = 131/279 (46.95%), Query Frame = 0

Query: 26   LWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIFFECPCAN 85
            LW L++ NK+KLF+WR    FLP    L +R V     C  CG   E+  H  + CP + 
Sbjct: 902  LWKLRIANKVKLFLWRACKGFLPCAANLFRRHVGNSSLCCRCGREEETTIHCMWSCPWSK 961

Query: 86   ALWKALDLSFHPESLKLLDTQSAIEFLGAGL---SKDNL-AYAVICLWSIWIDRNKVNFN 145
             +WK    SF     K     S I+     +   SK+ +  ++VIC W +W  RN+    
Sbjct: 962  KVWK---FSFLNGVYKQWREPSFIDLFAHVVKTSSKEEVELFSVIC-WLLWKSRNRFKHE 1021

Query: 146  EVVPPVNVRI----EWIRQYLEGVLKSNDMESHKHVEYDSCSTVAPVRWIAPWPGFLKLN 205
             VV      +    EW+  + +         S+++V  D+      V+W  P    LKLN
Sbjct: 1022 GVVEESKHIVWSANEWLNNFQDA------QRSNENVTKDATQKRVKVKWRPPTAPKLKLN 1081

Query: 206  VDASCSPLAPKLGFGLIFRDHMGLCKFASSIFKPVFCDILSAEALALLEGLKVADNLGFH 265
             DA+      K   G++ RD  G  K A     P    I++ EALA+  GL +    GF 
Sbjct: 1082 TDAAIDYKKKKADLGMVVRDGEGKLKCAGHKVLPGNPSIVAIEALAVYHGLLLYREAGFQ 1141

Query: 266  NLIIESDSKTLVDAILGNRLSLSPQGIILDEIRLLLKKY 297
            NL++ESDS  ++DA+    L LS +G +LD+I+ L   +
Sbjct: 1142 NLVVESDSTIVIDALNKTELDLSVEGGVLDDIKCLASTF 1170

BLAST of Sed0019957 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 8.5e-08
Identity = 55/255 (21.57%), Postives = 107/255 (41.96%), Query Frame = 0

Query: 25  CLWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIFFECPCA 84
           CLW ++VP ++K F+W + ++ + T     +R +     C +C    ES  H+  +CP  
Sbjct: 290 CLWKVRVPERVKTFLWLVGNQAVMTEEERHRRHLSASNVCQVCKGGVESMLHVLRDCPAQ 349

Query: 85  NALW-----KALDLSFHPESLKLLDTQSAIEFLGAGLSKDNLAYAVI---CLWSIWIDRN 144
             +W     +     F  +SL     +   + LG     +++ ++ I    +W  W  R 
Sbjct: 350 LGIWVRVVPQRRQQGFFSKSL----FEWLYDNLGDRSGCEDIPWSTIFAVIIWWGWKWRC 409

Query: 145 KVNFNEVVPPVNVRIEWIRQYLEGVLKSNDMESHKHVEYDSCSTVAPVRWIAPWPGFLKL 204
              F E       R+++++++   V +++    +  V          + W++P  G++K+
Sbjct: 410 GNIFGENT-KCRDRVKFVKEWAVEVYRAH--SGNVLVGITQPRVERMIGWVSPCVGWVKV 469

Query: 205 NVDASCSPLAPKLGFGLIFRDHMGL-C-KFASSIFKPVFCDILSAEALALLEGLKVADNL 264
           N D +          G + RD  G  C  F+ +I +   C    AE   +  GL  A   
Sbjct: 470 NTDGASRGNPGLASAGGVLRDCTGAWCGGFSLNIGR---CSAPQAELWGVYYGLYFAWEK 529

Query: 265 GFHNLIIESDSKTLV 270
               + +E DS+ +V
Sbjct: 530 KVPRVELEVDSEVIV 534

BLAST of Sed0019957 vs. ExPASy TrEMBL
Match: A0A392LXW3 (RNA-directed DNA polymerase (Reverse transcriptase) OS=Trifolium medium OX=97028 GN=A2U01_0000234 PE=4 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 9.7e-31
Identity = 97/286 (33.92%), Postives = 136/286 (47.55%), Query Frame = 0

Query: 12  CLDIIFLKPLGKKCLWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFY 71
           C+D+  L P  K  LWNLK+P ++  FMWRL H+ LPT + L  R +P    CV C    
Sbjct: 184 CVDL--LHPHWKN-LWNLKIPPRVSSFMWRLAHQCLPTRVNLTTRGIPCDDTCVSCETLA 243

Query: 72  ESFFHIFFECPCANALWKALDLSFHPESLKLLD---TQSAIEFLGAGLSKDNLAYAVICL 131
           ES  HIFF C  A   W  +DL  H   L L     T    +FL   LS      A + L
Sbjct: 244 ESHMHIFFVCSKAMECWGRIDLGHHVRELLLRTSEFTTLLFDFLDR-LSFIQQQLAAMLL 303

Query: 132 WSIWIDRN-KVNFNEVVPPVNVRIEWIRQYLEGVLKSNDMESHKHVEYDSCSTVAPVRWI 191
           WSIW  R+ K+  +    P  +         E           +H+ + +        W+
Sbjct: 304 WSIWKSRDLKLWESSSTSPTLIVTRAKETLHEWSCMQRIKPQVQHLAHTN-------TWV 363

Query: 192 APWPGFLKLNVDASCSPLAPKLGFGLIFRDHMGLCKFASSIFKPVFCDILSAEALALLEG 251
            P  G +K NVD +        GFG+ FRD +G   F  S   P F  +L AEA+ALL+ 
Sbjct: 364 KPTIGVIKCNVDVTTFNNNTMAGFGMCFRDSLGQFIFGKSAHLPSFGFVLEAEAVALLQS 423

Query: 252 LKVADNLGFHNLIIESDSKTLVDAILGNRLSLSPQGIILDEIRLLL 294
           +  A ++G H++I E+D+KTL +AI       +  G I+ + R+LL
Sbjct: 424 IHTAISIGLHSVIFETDNKTLANAINSTSAHNNEFGDIVTQCRVLL 458

BLAST of Sed0019957 vs. ExPASy TrEMBL
Match: A0A803P4U9 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 1.2e-28
Identity = 82/273 (30.04%), Postives = 132/273 (48.35%), Query Frame = 0

Query: 24   KCLWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIFFECPC 83
            K +W  ++  K+K+F+WR++H ++P    L KR + +   C  C +  E   H  +  P 
Sbjct: 1524 KVVWGSRLTPKMKIFIWRVFHHWIPVKTELTKRGMSMNLTCNRCKSQVEDVCHALWNIPK 1583

Query: 84   ANALWKALDLSFH-PESLKLLDTQSAIEFLGAGLSKDNLAYAVICLWSIWIDRNKVNFNE 143
             + +WK      H P SLK      A +FL     K +  + +   W IW  RNK  F  
Sbjct: 1584 LHKVWKHFGFLHHFPSSLK-----QAPDFLMLMKGKLSEEFFIGITWLIWYQRNKCVFQN 1643

Query: 144  VVPPVNVRIEWIRQYLEGVLKSNDMESHKHVEYDSCSTVAPVRWIAPWPGFLKLNVDASC 203
                 ++ I W  + +E  L S    SH+++  D+      V+W  P PG   +N DAS 
Sbjct: 1644 KDIEDDIWIPWATEMMEIHLASAQTNSHQNISKDT------VKWSPPPPGTFMINTDASL 1703

Query: 204  SPLAPKLGFGLIFRDHMGLCKFASSIFKPVFCDILSAEALALLEGLKVADNLGFHNLIIE 263
                P  G G+I RDH+G    A++ F P    +L AEA+A+   LK+A+     N+ I 
Sbjct: 1704 IEGQPGCGLGVIIRDHLGALVTATTEFIPGCLSVLLAEAMAIRLALKLAETRLMQNIFIA 1763

Query: 264  SDSKTLVDAILGNRLSLSPQGIILDEIRLLLKK 296
            SDS+++++A+ G     +  G ++D+     KK
Sbjct: 1764 SDSQSVINALKGKTHINTDWGFVIDDCIQASKK 1785

BLAST of Sed0019957 vs. ExPASy TrEMBL
Match: A0A7J6GGV5 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_001227 PE=4 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 1.2e-28
Identity = 82/273 (30.04%), Postives = 132/273 (48.35%), Query Frame = 0

Query: 24  KCLWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIFFECPC 83
           K +W  ++  K+K+F+WR++H ++P    L KR + +   C  C +  E   H  +  P 
Sbjct: 444 KVVWGSRLTPKMKIFIWRVFHHWIPVKTELTKRGMSMNLTCNRCKSQVEDVCHALWNIPK 503

Query: 84  ANALWKALDLSFH-PESLKLLDTQSAIEFLGAGLSKDNLAYAVICLWSIWIDRNKVNFNE 143
            + +WK      H P SLK      A +FL     K +  + +   W IW  RNK  F  
Sbjct: 504 LHKVWKHFGFLHHFPSSLK-----QAPDFLMLMKGKLSEEFFIGITWLIWYQRNKCVFQN 563

Query: 144 VVPPVNVRIEWIRQYLEGVLKSNDMESHKHVEYDSCSTVAPVRWIAPWPGFLKLNVDASC 203
                ++ I W  + +E  L S    SH+++  D+      V+W  P PG   +N DAS 
Sbjct: 564 KDIEDDIWIPWATEMMEIHLASAQTNSHQNISKDT------VKWSPPPPGTFMINTDASL 623

Query: 204 SPLAPKLGFGLIFRDHMGLCKFASSIFKPVFCDILSAEALALLEGLKVADNLGFHNLIIE 263
               P  G G+I RDH+G    A++ F P    +L AEA+A+   LK+A+     N+ I 
Sbjct: 624 IEGQPGCGLGVIIRDHLGALVTATTEFIPGCLSVLLAEAMAIRLALKLAETRLMQNIFIA 683

Query: 264 SDSKTLVDAILGNRLSLSPQGIILDEIRLLLKK 296
           SDS+++++A+ G     +  G ++D+     KK
Sbjct: 684 SDSQSVINALKGKTHINTDWGFVIDDCIQASKK 705

BLAST of Sed0019957 vs. ExPASy TrEMBL
Match: A0A803QI38 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 5.9e-28
Identity = 92/276 (33.33%), Postives = 131/276 (47.46%), Query Frame = 0

Query: 24   KCLWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIFFECPC 83
            K LWNL V  K+K F WR+YH  LP  L L  RK   QP C  C N  E+  H   +CP 
Sbjct: 1455 KTLWNLNVQPKIKNFAWRVYHHILPVGLNLFVRKTISQPTCSFCPNPTETVTHALLDCPR 1514

Query: 84   ANALWKALDLSFHPESLKLLDTQSAIEFLGAGLSKDNLAYAVICLWSIWIDRNKVNFNEV 143
            A+ +WKA  L     S + +D +  +      L KD L   V  LW+IW  RNK  F  +
Sbjct: 1515 ASKIWKASPLRSFYLSNRHVDVKEFMIIGFEQLHKDQLVLLVTTLWAIWYSRNKKLFANL 1574

Query: 144  VPPVNVRIEWIRQYLEGVLKSNDMES-HKHVEYDSCSTVAPVRWIAPWPGFLKLNVDASC 203
                N  I WI  Y+     +  M++ +  V     S   P   + P   +L L  DA+ 
Sbjct: 1575 DLTPNDTIAWIDSYISDYNAAMAMKNRYSGVLNFPASDRTPK--VVPQNQYL-LQTDAAI 1634

Query: 204  SPLAPKLGFGLIFRDHMGLCKFASSIFKPVFCDI--LSAEALALLEGLKVADNLGFHNLI 263
            +    K+GFG +  D  G  K  + +  PV  ++  L AEAL+L   L+   N+     +
Sbjct: 1635 NQTQSKMGFGAVLLDWQG--KVVAGLSAPVAGNLQPLIAEALSLRASLEWCINIQMPLAV 1694

Query: 264  IESDSKTLVDAILGNRLSLSPQGIILDEIRLLLKKY 297
            IE+DSK LVD +L  +   S    ++++IR  L  +
Sbjct: 1695 IETDSKLLVDKVLSKKADFSALADVVEDIRCSLSAF 1725

BLAST of Sed0019957 vs. ExPASy TrEMBL
Match: A0A803PV88 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 2.2e-27
Identity = 90/275 (32.73%), Postives = 131/275 (47.64%), Query Frame = 0

Query: 24   KCLWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIFFECPC 83
            K  WNL +P K+K F WR+YH  LP  L L +RK   QP C  C N  E+  H   +C  
Sbjct: 846  KTFWNLNLPPKVKNFSWRVYHHILPVALNLFRRKTIPQPHCSFCKNPVETVTHALLDCSR 905

Query: 84   ANALWKALDLSFHPESLKLLDTQSAIEFLGAG---LSKDNLAYAVICLWSIWIDRNKVNF 143
            A  +WKA        S + +D +   EF+  G   L+KD+L+  +  +W+IW  RNK  F
Sbjct: 906  AAKIWKASPFRTFYLSNRYVDVK---EFMLNGFDQLNKDSLSLLLSIMWAIWNSRNKKLF 965

Query: 144  -NEVVPPVNVRIEW----IRQYLEGVLKSNDMESHKHVEYDSCSTVAPVRWIAPWPGFLK 203
             N  + P ++ + W    I  Y E +   N  +S + ++ +      PV          +
Sbjct: 966  ANSDMAPTDI-VAWTHTFISDYQEALSNVNRYKSARILQRNDIDVTVPV-------NSYR 1025

Query: 204  LNVDASCSPLAPKLGFGLIFRDHMGLCKFASSIFKPVFCDILSAEALALLEGLKVADNLG 263
            LN DA+ S    KLG G + +D  G      +I          AEALAL  GL    ++ 
Sbjct: 1026 LNTDAALSTERSKLGIGAVVKDWKGCVVANLAIPAAGALQSTLAEALALKAGLNWCQHIK 1085

Query: 264  FHNLIIESDSKTLVDAILGNRLSLSPQGIILDEIR 291
                ++E+DSK LVD +LG +  LS    I+ +IR
Sbjct: 1086 IPLALVETDSKLLVDKVLGGKNDLSALSDIVTDIR 1109

BLAST of Sed0019957 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 87.8 bits (216), Expect = 1.6e-17
Identity = 71/258 (27.52%), Postives = 103/258 (39.92%), Query Frame = 0

Query: 23  KKCLWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIFFECP 82
           K  +WNL +  KLK F+WR   + L T   L  R + + P C  C    ES  H  F CP
Sbjct: 159 KTRIWNLPIMPKLKHFLWRALSQALATTERLTTRGMRIDPSCPRCHRENESINHALFTCP 218

Query: 83  CANALWKALDLSFHPESLKLLDTQSAIEFL-----GAGLSKDNLAYAVICLWSIWIDRNK 142
            A   W+  D S     L   D +  I  +        +S  +    V  +W IW  RN 
Sbjct: 219 FATMAWRLSDSSLIRNQLMSNDFEENISNILNFVQDTTMSDFHKLLPVWLIWRIWKARNN 278

Query: 143 VNFNEVVPPVNVRIEWIRQYLEGVLKSNDMESHKHVEYDSCSTVA-PVRWIAPWPGFLKL 202
           V FN+     +  +   +      L  N  +SHK     +       + W  P   ++K 
Sbjct: 279 VVFNKFRESPSKTVLSAKAETHDWL--NATQSHKKTPSPTRQIAENKIEWRNPPATYVKC 338

Query: 203 NVDASCSPLAPKLGFGLIFRDHMGLCKFASSIFKPVFCDILSAEALALLEGLKVADNLGF 262
           N DA       +   G I R+H G      S+      + L AE  ALL  L+     G+
Sbjct: 339 NFDAGFDVQKLEATGGWIIRNHYGTPISWGSMKLAHTSNPLEAETKALLAALQQTWIRGY 398

Query: 263 HNLIIESDSKTLVDAILG 275
             + +E D +TL++ I G
Sbjct: 399 TQVFMEGDCQTLINLING 414

BLAST of Sed0019957 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 85.9 bits (211), Expect = 6.0e-17
Identity = 61/264 (23.11%), Postives = 110/264 (41.67%), Query Frame = 0

Query: 23  KKCLWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIFFECP 82
           K  +W LK   K+K F+W+L    L T   L++R +   P C  C    E+  H+FF+C 
Sbjct: 15  KAKIWKLKTAPKIKHFLWKLLSGALATGDNLKRRHIRNHPQCHRCCQEDETSQHLFFDCF 74

Query: 83  CANALWKALDLSFHPESLKLLDTQSAIEFL----GAGLSKDNLAYAVICLWSIWIDRNKV 142
            A  +W+A  +         +  ++ +E L     A         A+  LW +W  RN++
Sbjct: 75  YAQQVWRASGIPHQELRTTGITMETKMELLLSSCLANRQPQLFNLAIWILWRLWKSRNQL 134

Query: 143 NFNEVVPPVNVRIEWIRQYLEGVLKSN----DMESHKHVEYDSCSTVAPVRWIAPWPGFL 202
            F +        ++  R  ++    +N     +    H       T+A  +W  P   ++
Sbjct: 135 VFQQKSISWQNTLQRARNDVQEWEDTNTYVQSLNQQVHSSRHQQPTMARTKWQRPPSTWI 194

Query: 203 KLNVDASCSPLAPKLGFGLIFRDHMGLCKFASSIFKPVFCDILSAEALALLEGLKVADNL 262
           K N D + +        G + RD  G+   +         D L +E  AL+  ++ A + 
Sbjct: 195 KYNYDGAFNHQTRNAKAGWLMRDENGVYMGSGQAIGSTTSDSLESEFQALIIAMQHAWSQ 254

Query: 263 GFHNLIIESDSKTLVDAILGNRLS 279
           G+  +I E DSK + + +   +L+
Sbjct: 255 GYRKVIFEGDSKQVEELMNNEKLN 278

BLAST of Sed0019957 vs. TAIR 10
Match: AT1G10000.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 5.1e-08
Identity = 78/284 (27.46%), Postives = 120/284 (42.25%), Query Frame = 0

Query: 34  KLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIFFECPCANALWK--AL 93
           K+KLF+W+     LP    L +R +     C  CG   E+  H+ F C  A  +W    L
Sbjct: 3   KIKLFLWKAAAGALPVGAQLVRRHISSTWDCARCG-APETSTHMLFHCDFAAQVWNLAPL 62

Query: 94  DLSFHP------ESLKLLDTQSAIEFLGAGLSKDNLAYAVICLWSIWIDRNKVNFNEVVP 153
            L   P      E+L LL  +  I     G+    L +  IC W IW  RN++ F     
Sbjct: 63  QLGHIPIGTPILEALNLL--KKTIVLPPVGIHTATL-FPWIC-WHIWKARNQLIFQNSHF 122

Query: 154 PVNVRIEWIRQYLEGVLKSNDMESHKHVEYDSCSTVAP-VRWIAPWPG--------FLKL 213
            V   IE + + ++  L            + S     P VR   P P         FL  
Sbjct: 123 SV---IETVTKAVQDAL-----------AWQSAQLALPKVRVATPRPNSTTTLTHDFL-C 182

Query: 214 NVDASCSPLAPKLGFGLIFR----DHMGLCKFASSIFKPVFCDILSAEALALLEGLKVAD 273
            VDA+    +   G G +F+        +  F++   +  F   L+AEA A+   +  A 
Sbjct: 183 YVDAAWQQQSSLAGSGWVFQATSHSEKEITTFSAGCRR--FPSPLAAEAWAIKSAMLHAL 242

Query: 274 NLGFHNLIIESDSKTLVDAILGNRLSLSPQGIILDEIRLLLKKY 297
            L   +L++ SDSK++VDA L + +SL+    +L EIR +  ++
Sbjct: 243 QLERSDLLVLSDSKSIVDA-LNSNVSLNEIFGLLVEIRSIRNRF 263

BLAST of Sed0019957 vs. TAIR 10
Match: AT3G26855.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 54.7 bits (130), Expect = 1.5e-07
Identity = 22/59 (37.29%), Postives = 35/59 (59.32%), Query Frame = 0

Query: 26 LWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIFFECPCA 85
          +W+LK+  K+KL +W+  +  LP    L  R + ++P+C  C +F E+  HI F CP A
Sbjct: 9  IWSLKISPKIKLLIWKALNNALPVGAQLLSRNISIEPFCTRCRDF-ETITHILFNCPFA 66

BLAST of Sed0019957 vs. TAIR 10
Match: AT5G18880.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 42.4 bits (98), Expect = 7.6e-04
Identity = 22/74 (29.73%), Postives = 35/74 (47.30%), Query Frame = 0

Query: 24  KCLWNLKVPNKLKLFMWRLYHEFLPTNLVLQKRKVPVQPWCVMCGNFYESFFHIFFECPC 83
           K +W  +   +  L  W  + E LPT   L+   + +    V+C N  E+  H+FFEC  
Sbjct: 129 KVVWFKEYIPRFSLITWMSFLERLPTRDRLRGWGMNIPSSWVLCSNGDETHAHLFFECSF 188

Query: 84  ANALWKALDLSFHP 98
           + A+W+     F P
Sbjct: 189 SLAIWEFFASKFRP 202

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MCH79484.12.0e-3033.92RNA-directed DNA polymerase (Reverse transcriptase) [Trifolium medium][more]
XP_031094430.11.4e-2830.69uncharacterized protein LOC115998901 [Ipomoea triloba][more]
KAF4382082.12.4e-2830.04hypothetical protein F8388_001227 [Cannabis sativa][more]
XP_019163602.14.6e-2730.74PREDICTED: uncharacterized protein LOC109159944 [Ipomoea nil][more]
XP_024190234.17.9e-2731.54uncharacterized protein LOC112194221 [Rosa chinensis][more]
Match NameE-valueIdentityDescription
P0C2F68.5e-0821.57Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Match NameE-valueIdentityDescription
A0A392LXW39.7e-3133.92RNA-directed DNA polymerase (Reverse transcriptase) OS=Trifolium medium OX=97028... [more]
A0A803P4U91.2e-2830.04Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A7J6GGV51.2e-2830.04Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_001227 PE=4 SV=1[more]
A0A803QI385.9e-2833.33Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803PV882.2e-2732.73Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G09510.11.6e-1727.52Ribonuclease H-like superfamily protein [more]
AT3G25270.16.0e-1723.11Ribonuclease H-like superfamily protein [more]
AT1G10000.15.1e-0827.46Ribonuclease H-like superfamily protein [more]
AT3G26855.11.5e-0737.29RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT5G18880.17.6e-0429.73RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 197..295
e-value: 2.4E-15
score: 56.5
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 193..296
e-value: 8.5E-9
score: 37.5
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 23..88
e-value: 5.0E-18
score: 65.6
NoneNo IPR availablePANTHERPTHR46736FAMILY NOT NAMEDcoord: 23..279
NoneNo IPR availablePANTHERPTHR46736:SF6SUBFAMILY NOT NAMEDcoord: 23..279
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 196..295
e-value: 4.94E-21
score: 84.288
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 193..274

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0019957.1Sed0019957.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity