Homology
BLAST of Sed0019841 vs. NCBI nr
Match:
KAG6590040.1 (Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1364.7 bits (3531), Expect = 0.0e+00
Identity = 679/765 (88.76%), Postives = 717/765 (93.73%), Query Frame = 0
Query: 1 MDSMARFLILSLLLLLLLPCAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHSS 60
M SMAR L+ S LLLLLLPC +NAK+TYIV+MKH LPS+Y THHDW+SAHLQSLS S+
Sbjct: 1 MGSMARLLVASFLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSA 60
Query: 61 TSDSLLYTYTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSNF 120
TSDSLLYTYTSAYHGFAASLDPDE ELL QSDSVLGVYED+VY LHTTRTP FLGLDS+F
Sbjct: 61 TSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
Query: 121 NLWEGHNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPSL 180
LWEGHNTQDL+QASHDVIIGVLDTGIWP SKSFDD GMPEIP+RWRGECESGPDFSPSL
Sbjct: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180
Query: 181 CNKKLIGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLGY 240
CNKKLIGARSF KGY+MASGGGY ++ ENESP+D+DGHGTHTASTAAGSHVANASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLGY 240
Query: 241 ASGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHRDT 300
A GIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRA+SDGVDVLSLSLGGGSAPY+RDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
Query: 361 VGVSLYSGEGMGKQPVGLVYSKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKGG 420
GVSLYSG+GMG +PV LVY+KGSNSSSN+CL GSLEP V+GKVV+CDRG+NARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGG 420
Query: 421 VVRQAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFGG 480
VVR AGG+GMILANTAASGEELVADSHLLPAVAVG TGDLIRQYV S A+PTA LSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGG 480
Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQF 540
TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD DKRKTQF
Sbjct: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQF 540
Query: 541 NIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELSN 600
NIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYT DNTNSSLRDAAGG SN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600
Query: 601 PWAHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCSKKFADP 660
PWAHG+GHVDPHKALSPGLVYDIST++YI FLCSLDYG+DHVQAI K SN+TC KKFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFADP 660
Query: 661 GQLNYPSFSVVFGSKRVVRYTREVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVGE 720
GQLNYPSFSVVFGSKRVVRYTR VTNVGAA SVYEVATTAPSVVKV VKPSKLVF+KVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGE 720
Query: 721 KQRYTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAWTRL 766
++RYTVTFVASR AA+T R+GFGSIAWSNDQHQVRSPVAFAWT+L
Sbjct: 721 RKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 765
BLAST of Sed0019841 vs. NCBI nr
Match:
XP_022987083.1 (subtilisin-like protease SBT1.8 [Cucurbita maxima])
HSP 1 Score: 1363.6 bits (3528), Expect = 0.0e+00
Identity = 681/766 (88.90%), Postives = 720/766 (93.99%), Query Frame = 0
Query: 1 MDSMARFLILS-LLLLLLLPCAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHS 60
M SMAR L+ S LLLLLLLPC +NAK+TYIV+MKH LPS+Y THHDW+SAHLQSLS S
Sbjct: 1 MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60
Query: 61 STSDSLLYTYTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSN 120
+TSDSLLYTYTSAYHGFAASLDPDE ELL QSDSVLGVYED+VY LHTTRTP FLGLDS+
Sbjct: 61 ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDSD 120
Query: 121 FNLWEGHNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPS 180
F LWEGHNTQDL+QASHDVIIGVLDTGIWP SKSFDD GMPEIP+RWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
Query: 181 LCNKKLIGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLG 240
LCNKKLIGARSF KGY+MASGGGY ++ ENESP+D+DGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
Query: 241 YASGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHRD 300
YA GIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRA+SDGVDVLSLSLGGGSAPY+RD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
Query: 361 FVGVSLYSGEGMGKQPVGLVYSKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKG 420
F GVSLYSG+GMG +PV LVY+KGSNSSSN+CL GSLEP V+GKVV+CDRG+NARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420
Query: 421 GVVRQAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFG 480
GVVR AGG+GMILANTAASGEELVADSHLLPAVAVG TGDLIRQYV S A+PTA LSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480
Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQ 540
GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540
Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELS 600
FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYT DNTNSSLRDAAGG S
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFS 600
Query: 601 NPWAHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCSKKFAD 660
NPWAHG+GHVDPHKALSPGL+YDIST++YI FLCSLDYG+DHVQAIVK SN+TCSKKFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKKFAD 660
Query: 661 PGQLNYPSFSVVFGSKRVVRYTREVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVG 720
PGQLNYPSFSVVFGSKRVVRYTR VTNVGAA SVYEVATTAPSVVKV VKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720
Query: 721 EKQRYTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAWTRL 766
E++RYTVTFVASR AA+T R+GFGSIAWSNDQHQVRSPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766
BLAST of Sed0019841 vs. NCBI nr
Match:
XP_023516159.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1362.1 bits (3524), Expect = 0.0e+00
Identity = 678/765 (88.63%), Postives = 716/765 (93.59%), Query Frame = 0
Query: 1 MDSMARFLILSLLLLLLLPCAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHSS 60
M SMA L+ LLLLLLPC +NAK+TYIV+MKH LPS+Y THHDW+SAHLQSLS S+
Sbjct: 1 MGSMASLLVAYFLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSA 60
Query: 61 TSDSLLYTYTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSNF 120
TSDSLLYTYTSAYHGFAASLDPDE ELL QSDSVLGVYED+VY LHTTRTP FLGLDS+F
Sbjct: 61 TSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
Query: 121 NLWEGHNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPSL 180
LWEGHNTQDL+QASHDVIIGVLDTGIWP SKSFDD GMPEIP+RWRGECESGPDFSPSL
Sbjct: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180
Query: 181 CNKKLIGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLGY 240
CNKKLIGARSF KGY+MASGGGY ++ ENESP+D+DGHGTHTASTAAGSHVANASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLGY 240
Query: 241 ASGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHRDT 300
A GIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRA+SDGVDVLSLSLGGGSAPY+RDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
Query: 361 VGVSLYSGEGMGKQPVGLVYSKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKGG 420
GVSLYSG+GMG +PV LVY+KGSNSSSN+CL GSLEP V+GKVV+CDRG+NARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGG 420
Query: 421 VVRQAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFGG 480
VVR AGG+GMILANTAASGEELVADSHLLPAVAVG TGDLIRQYV S A+PTA LSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGG 480
Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQF 540
TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD DKRKTQF
Sbjct: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQF 540
Query: 541 NIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELSN 600
NIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYT DNTNSSLRDAAGG SN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFSN 600
Query: 601 PWAHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCSKKFADP 660
PWAHG+GHVDPHKALSPGLVYDIST++YI FLCSLDYG+DHVQAI K SN+TCSKKFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCSKKFADP 660
Query: 661 GQLNYPSFSVVFGSKRVVRYTREVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVGE 720
GQLNYPSFSVVFGSKRVVRYTR VTNVGAA SVYEVATTAPSVVKV VKPSKLVF+KVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGE 720
Query: 721 KQRYTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAWTRL 766
++RYTVTFVASR AA+T R+GFGSIAWSNDQHQVRSPVAFAWT+L
Sbjct: 721 RKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 765
BLAST of Sed0019841 vs. NCBI nr
Match:
XP_022960792.1 (subtilisin-like protease SBT1.8 [Cucurbita moschata] >KAG7023704.1 Subtilisin-like protease SBT1.8 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1361.7 bits (3523), Expect = 0.0e+00
Identity = 680/766 (88.77%), Postives = 718/766 (93.73%), Query Frame = 0
Query: 1 MDSMARFLILS-LLLLLLLPCAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHS 60
M SMAR L+ S LLLLLLLPC +NAK+TYIV+MKH LPS+Y THHDW+SAHLQSLS S
Sbjct: 1 MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60
Query: 61 STSDSLLYTYTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSN 120
+TSDSLLYTYTSAYHGFAASLDPDE ELL QSDSVLGVYED+VY LHTTRTP FLGLDS+
Sbjct: 61 ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
Query: 121 FNLWEGHNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPS 180
F LWEGHNTQDL+QASHDVIIGVLDTGIWP SKSFDD GMPEIP+RWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
Query: 181 LCNKKLIGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLG 240
LCNKKLIGARSF KGY+MASGGGY ++ ENESP+D+DGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
Query: 241 YASGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHRD 300
YA GIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRA+SDGVDVLSLSLGGGSAPY+RD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
Query: 361 FVGVSLYSGEGMGKQPVGLVYSKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKG 420
F GVSLYSG+GMG +PV LVY+KGSNSSSN+CL GSLEP V+GKVV+CDRG+NARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420
Query: 421 GVVRQAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFG 480
GVVR AGG+GMILANTAASGEELVADSHLLPAVAVG TGDLIRQYV S A+PTA LSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480
Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQ 540
GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540
Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELS 600
FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYT DNTNSSLRDAAGG S
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
Query: 601 NPWAHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCSKKFAD 660
NPWAHG+GHVDPHKALSPGLVYDIST++YI FLCSLDYG+DHVQAI K SN+TC KKFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660
Query: 661 PGQLNYPSFSVVFGSKRVVRYTREVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVG 720
PGQLNYPSFSVVFGSKRVVRYTR VTNVGAA SVYEVATTAPSVVKV VKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720
Query: 721 EKQRYTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAWTRL 766
E++RYTVTFVASR AA+T R+GFGSIAWSNDQHQVRSPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766
BLAST of Sed0019841 vs. NCBI nr
Match:
XP_038879224.1 (subtilisin-like protease SBT1.8 [Benincasa hispida])
HSP 1 Score: 1359.0 bits (3516), Expect = 0.0e+00
Identity = 682/765 (89.15%), Postives = 718/765 (93.86%), Query Frame = 0
Query: 1 MDSMARFLILSLLLLLLLPCAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHSS 60
M SMAR LI LLLLLPC +NAKKTYIV+MKH+ LPSEY THHDW+SAHLQSLS SS
Sbjct: 1 MGSMARLLI--AFLLLLLPCVFVNAKKTYIVRMKHNALPSEYLTHHDWYSAHLQSLSSSS 60
Query: 61 TSDSLLYTYTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSNF 120
TSDSLLYTYTSAYHGFAASLD +E ELL QSDSVLGVYEDSVYTLHTTRTP FLGLDS+F
Sbjct: 61 TSDSLLYTYTSAYHGFAASLDSNEAELLRQSDSVLGVYEDSVYTLHTTRTPGFLGLDSDF 120
Query: 121 NLWEGHNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPSL 180
LWEGHNTQDLNQASHDVIIGVLDTGIWP SKSFDD GMPEIP+RWRGECESG DFSPSL
Sbjct: 121 GLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGADFSPSL 180
Query: 181 CNKKLIGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLGY 240
CNKKLIGARSF KGY+MASGGGY + ENESP+D+DGHGTHTASTAAGSHVANASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGY 240
Query: 241 ASGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHRDT 300
A GIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRA+SDGVDVLSLSLGGGSAPY+RDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQ+GNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQIGNGKRF 360
Query: 361 VGVSLYSGEGMGKQPVGLVYSKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKGG 420
GVSLYSG+GMG + V LVY+KGSN+SSN+CL GSLEP +V+GKVVVCDRG+NARVEKGG
Sbjct: 361 TGVSLYSGQGMGSKAVELVYNKGSNTSSNMCLPGSLEPAVVRGKVVVCDRGINARVEKGG 420
Query: 421 VVRQAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFGG 480
VVR AGG+GMILANTAASGEELVADSHLLPAVAVGR TGDLIRQYV S + PTA LSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480
Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQF 540
T+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD DKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQF 540
Query: 541 NIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELSN 600
NIMSGTSMSCPHISGLAALLKAAHP+WSPSAIKSALMTTAYT DNTNSSLRDAAGG SN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600
Query: 601 PWAHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCSKKFADP 660
PWAHG+GHVDPHKALSPGL+YDIST++YIAFLCSLDYG+DHVQAIVK SN+TCS+KFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660
Query: 661 GQLNYPSFSVVFGSKRVVRYTREVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVGE 720
GQLNYPSFSVVFGSKRVVRYTR VTNVGAA SVYEVATTAPSVVKV VKPSKLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFTKVGE 720
Query: 721 KQRYTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAWTRL 766
++RYTVTFVASR AAQTMRFGFGSIAW+N QHQVRSPVAFAWTRL
Sbjct: 721 RKRYTVTFVASRDAAQTMRFGFGSIAWNNYQHQVRSPVAFAWTRL 763
BLAST of Sed0019841 vs. ExPASy Swiss-Prot
Match:
Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)
HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 544/763 (71.30%), Postives = 633/763 (82.96%), Query Frame = 0
Query: 3 SMARFLILSLLLLLLLPCAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHSSTS 62
S + I++ L LLL AKKTYI+++ HS P + THHDW+++ L S S
Sbjct: 7 SSSSITIITTFLFLLLH---TTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSES----- 66
Query: 63 DSLLYTYTSAYHGFAASLDPDEVE-LLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSNFN 122
SLLYTYT+++HGF+A LD E + LLS S+S+L ++ED +YTLHTTRTPEFLGL+S F
Sbjct: 67 -SLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFG 126
Query: 123 LWEGHNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPSLC 182
+ DL +S+ VIIGVLDTG+WP S+SFDD MPEIP++W+GECESG DF LC
Sbjct: 127 V------HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLC 186
Query: 183 NKKLIGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLGYA 242
NKKLIGARSF KG++MASGGG+ + E+ SP+D DGHGTHT++TAAGS V NAS LGYA
Sbjct: 187 NKKLIGARSFSKGFQMASGGGFSSKR-ESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYA 246
Query: 243 SGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHRDTI 302
+G ARGMA +ARVA YK CW TGCFGSDILA MDRA+ DGVDVLSLSLGGGSAPY+RDTI
Sbjct: 247 AGTARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTI 306
Query: 303 AIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFV 362
AIGAF+AME+GVFVSCSAGNSGP +AS+ANVAPW+MTVGAGTLDRDFPA+ LGNGKR
Sbjct: 307 AIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLT 366
Query: 363 GVSLYSGEGMGKQPVGLVYSKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKGGV 422
GVSLYSG GMG +P+ LVY+KG++SSSNLCL GSL+ +V+GK+VVCDRGVNARVEKG V
Sbjct: 367 GVSLYSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAV 426
Query: 423 VRQAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFGGT 482
VR AGG+GMI+ANTAASGEELVADSHLLPA+AVG+ TGDL+R+YV+S ++PTA L F GT
Sbjct: 427 VRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGT 486
Query: 483 VLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQFN 542
VL+V+PSPVVAAFSSRGPN VTP+ILKPDVIGPGVNILA WS++IGPTGLD D R+TQFN
Sbjct: 487 VLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFN 546
Query: 543 IMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELSNP 602
IMSGTSMSCPHISGLA LLKAAHPEWSPSAIKSALMTTAY LDNTN+ L DAA LSNP
Sbjct: 547 IMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNP 606
Query: 603 WAHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCSKKFADPG 662
+AHGSGHVDP KALSPGLVYDIST+EYI FLCSLDY +DH+ AIVK +V CSKKF+DPG
Sbjct: 607 YAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPG 666
Query: 663 QLNYPSFSVVFGSKRVVRYTREVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVGEK 722
QLNYPSFSV+FG KRVVRYTREVTNVGAA+SVY+V V + VKPSKL F VGEK
Sbjct: 667 QLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEK 726
Query: 723 QRYTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAWTR 765
+RYTVTFV+ +G + T + FGSI WSN QH+VRSPVAF+W R
Sbjct: 727 KRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNR 753
BLAST of Sed0019841 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 786.6 bits (2030), Expect = 2.6e-226
Identity = 415/760 (54.61%), Postives = 534/760 (70.26%), Query Frame = 0
Query: 13 LLLLLLPCAIINAKK---TYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHSSTSDSLLYTY 72
LLL L C + ++ TYIV M S +PS + H +W+ + L+S+S S+ LLYTY
Sbjct: 13 LLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA---ELLYTY 72
Query: 73 TSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSNFNLWEGHNTQ 132
+A HGF+ L +E + L V+ V + Y LHTTRTP FLGLD +T
Sbjct: 73 ENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD--------EHTA 132
Query: 133 DLNQAS---HDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPSLCNKKLI 192
DL + DV++GVLDTG+WP SKS+ D G IP+ W+G CE+G +F+ SLCN+KLI
Sbjct: 133 DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLI 192
Query: 193 GARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLGYASGIAR 252
GAR F +GY S G + + E+ SP+D+DGHGTHT+STAAGS V ASLLGYASG AR
Sbjct: 193 GARFFARGYE--STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTAR 252
Query: 253 GMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHRDTIAIGAF 312
GMAP+ARVA YK CW GCF SDILA +D+A++D V+VLS+SLGGG + Y+RD +AIGAF
Sbjct: 253 GMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAF 312
Query: 313 AAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFVGVSLY 372
AAME+G+ VSCSAGN+GP+ +SL+NVAPWI TVGAGTLDRDFPA LGNGK F GVSL+
Sbjct: 313 AAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLF 372
Query: 373 SGEGMGKQPVGLVYSKGSNSSS--NLCLVGSLEPGMVKGKVVVCDRGVNARVEKGGVVRQ 432
GE + + + +Y+ +++++ NLC+ G+L P VKGK+V+CDRG+NARV+KG VV+
Sbjct: 373 KGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKA 432
Query: 433 AGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFGGTVLN 492
AGGVGMILANTAA+GEELVAD+HLLPA VG GD+IR YV + PTA++S GTV+
Sbjct: 433 AGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG 492
Query: 493 VRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQFNIMS 552
V+PSPVVAAFSSRGPN +TP ILKPD+I PGVNILAAW+ + GPTGL +D R+ +FNI+S
Sbjct: 493 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIIS 552
Query: 553 GTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELSNPWAH 612
GTSMSCPH+SGLAALLK+ HPEWSP+AI+SALMTTAY L D A G+ S P+ H
Sbjct: 553 GTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDH 612
Query: 613 GSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCS-KKFADPGQL 672
G+GHV P A +PGL+YD++T++Y+ FLC+L+Y +++ V N TC K L
Sbjct: 613 GAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRS-VSRRNYTCDPSKSYSVADL 672
Query: 673 NYPSFSVVFGSKRVVRYTREVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVGEKQR 732
NYPSF+V +YTR VT+VG A + T+ + VK+ V+P+ L F + EK+
Sbjct: 673 NYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKS 732
Query: 733 YTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAWT 764
YTVTF FGSI WS+ +H V SPVA +WT
Sbjct: 733 YTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISWT 757
BLAST of Sed0019841 vs. ExPASy Swiss-Prot
Match:
Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)
HSP 1 Score: 754.6 bits (1947), Expect = 1.1e-216
Identity = 410/768 (53.39%), Postives = 515/768 (67.06%), Query Frame = 0
Query: 7 FLILSLLLLLLLP-CAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHSSTSDSL 66
F + LLL P + + ++YIV ++ S PS + +H++W + L+SL S +L
Sbjct: 10 FFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATL 69
Query: 67 LYTYTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSNFNLWEG 126
LY+Y+ A HGF+A L P + L + SV+ V D +HTT TP FLG N LW
Sbjct: 70 LYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSN 129
Query: 127 HNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPSLCNKKL 186
N DVI+GVLDTGIWP SF D+G+ IP+ W+GECE GPDF S CN+KL
Sbjct: 130 SN------YGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKL 189
Query: 187 IGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLGYASGIA 246
IGAR+F++GY G A E+ SP+D +GHGTHTASTAAGS VANASL YA G A
Sbjct: 190 IGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTA 249
Query: 247 RGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLG-GGSAP-YHRDTIAI 306
GMA +AR+AAYK CW GC+ SDILA MD+A++DGV V+SLS+G GSAP YH D+IAI
Sbjct: 250 TGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAI 309
Query: 307 GAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFVGV 366
GAF A G+ VSCSAGNSGPN + N+APWI+TVGA T+DR+F A G+GK F G
Sbjct: 310 GAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGT 369
Query: 367 SLYSGEGMGKQPVGLVYSKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKGGVVR 426
SLY+GE + + LVYS + S LC G L +V+GK+V+CDRG NARVEKG V+
Sbjct: 370 SLYAGESLPDSQLSLVYS--GDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVK 429
Query: 427 QAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFGGTVL 486
AGG GMILANTA SGEEL ADSHL+PA VG GD IR Y++++ PTA +SF GT++
Sbjct: 430 LAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLI 489
Query: 487 NVR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQFNI 546
PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+ +GPT LD D R+ QFNI
Sbjct: 490 GPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNI 549
Query: 547 MSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELSNPW 606
+SGTSMSCPH+SGLAALL+ AHP+WSP+AIKSAL+TTAY ++N+ + D A G+ SN +
Sbjct: 550 ISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSF 609
Query: 607 AHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNV--TC-SKKFAD 666
HG+GHVDP+KAL+PGLVYDI EY+AFLC++ Y + ++ + C + K
Sbjct: 610 IHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRT 669
Query: 667 PGQLNYPSFSVVFGSK-RVVRYTREVTNVGA-AASVYEVATTAPSVVKVVVKPSKLVFTK 726
G LNYPSFSVVF S VV+Y R V NVG+ +VYEV +P+ V++ V PSKL F+K
Sbjct: 670 AGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSK 729
Query: 727 VGEKQRYTVTF---VASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAW 763
Y VTF V G FGSI W++ +H V+SPVA W
Sbjct: 730 EKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769
BLAST of Sed0019841 vs. ExPASy Swiss-Prot
Match:
Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)
HSP 1 Score: 735.7 bits (1898), Expect = 5.2e-211
Identity = 393/774 (50.78%), Postives = 516/774 (66.67%), Query Frame = 0
Query: 7 FLILSLLLLLLL--PCAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLS-HSS--- 66
F+ILS+ L+ L I+ KKTY++ M S +P Y H W+S+ + S++ H S
Sbjct: 13 FIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEE 72
Query: 67 --TSDSLLYTYTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDS 126
++ +LYTY +A+HG AA L +E E L + D V+ V ++ Y LHTTR+P FLGL+
Sbjct: 73 EGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLER 132
Query: 127 NFNLWEGHNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSP 186
E HDV++GVLDTGIWP S+SF+D GM +PA WRG CE+G F
Sbjct: 133 Q----ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLK 192
Query: 187 SLCNKKLIGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLL 246
CN+K++GAR F++GY A+ G + E +SP+D DGHGTHTA+T AGS V A+L
Sbjct: 193 RNCNRKIVGARVFYRGYEAAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLF 252
Query: 247 GYASGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHR 306
G+A G ARGMA +ARVAAYK CW GCF SDIL+ +D+A++DGV VLS+SLGGG + Y R
Sbjct: 253 GFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSR 312
Query: 307 DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGK 366
D+++I F AME GVFVSCSAGN GP+ SL NV+PWI TVGA T+DRDFPA V++G +
Sbjct: 313 DSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMR 372
Query: 367 RFVGVSLYSGEGM--GKQPVGLVYSKGSNSSS----NLCLVGSLEPGMVKGKVVVCDRGV 426
F GVSLY G + + LVY G N+SS + CL G+L+ V GK+V+CDRGV
Sbjct: 373 TFKGVSLYKGRTVLPKNKQYPLVY-LGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGV 432
Query: 427 NARVEKGGVVRQAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEP 486
RV+KG VV++AGG+GM+L NTA +GEELVADSH+LPAVAVG G LI+QY ++ +
Sbjct: 433 TPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKA 492
Query: 487 TAALSFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD 546
TA+L GT + ++PSPVVAAFSSRGPN ++ +ILKPD++ PGVNILAAW+ + P+ L
Sbjct: 493 TASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLS 552
Query: 547 TDKRKTQFNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRD 606
+D R+ +FNI+SGTSMSCPH+SG+AAL+K+ HP+WSP+AIKSALMTTAY DN L D
Sbjct: 553 SDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTD 612
Query: 607 AAGGELSNPWAHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVT 666
A+G S+P+ HG+GH+DP +A PGLVYDI EY FLC+ D ++ KHSN T
Sbjct: 613 ASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRT 672
Query: 667 CSKKFA-DPGQLNYPSFSVVFGSK---RVVRYTREVTNVGAAASVYEVATTAPSVVKVVV 726
C A +PG LNYP+ S +F + + R VTNVG S Y+V+ + V V
Sbjct: 673 CKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTV 732
Query: 727 KPSKLVFTKVGEKQRYTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAW 763
+P L FT +K YTVTF R + R FG + W + H+VRSPV W
Sbjct: 733 QPKTLNFTSKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776
BLAST of Sed0019841 vs. ExPASy Swiss-Prot
Match:
O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)
HSP 1 Score: 715.7 bits (1846), Expect = 5.6e-205
Identity = 381/762 (50.00%), Postives = 513/762 (67.32%), Query Frame = 0
Query: 12 LLLLLLLP---CAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHSSTSDSLLYT 71
LLL L P A A KT+I ++ +PS +PTH+ W+S S +++
Sbjct: 8 LLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESR------IVHV 67
Query: 72 YTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSNFNLWEGHNT 131
Y + +HGF+A + PDE + L +VL V+ED LHTTR+P+FLGL + LW
Sbjct: 68 YHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSE--- 127
Query: 132 QDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPSLCNKKLIGA 191
+ DVIIGV DTGIWP +SF D + IP RWRG CESG FSP CN+K+IGA
Sbjct: 128 ---SDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGA 187
Query: 192 RSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLGYASGIARGM 251
R F KG + A GG + + E SP+D DGHGTHT+STAAG H AS+ GYASG+A+G+
Sbjct: 188 RFFAKGQQAAVIGG-INKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGV 247
Query: 252 APQARVAAYKTCW-PTGCFGSDILAGMDRALSDGVDVLSLSLGGG---SAPYHRDTIAIG 311
AP+AR+AAYK CW +GC SDILA D A+ DGVDV+S+S+GGG ++PY+ D IAIG
Sbjct: 248 APKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIG 307
Query: 312 AFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFVGVS 371
++ A KG+FVS SAGN GPN S+ N+APW+ TVGA T+DR+FPA LG+G R GVS
Sbjct: 308 SYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVS 367
Query: 372 LYSGEGMGKQPVGLVY-SKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKGGVVR 431
LY+G + + +VY K SS++LC+ +L+P V+GK+V+CDRG + RV KG VV+
Sbjct: 368 LYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVK 427
Query: 432 QAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFGGTVL 491
+AGGVGMILAN A++GE LV D+HL+PA AVG N GD I+ Y S P A++ F GT++
Sbjct: 428 KAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIV 487
Query: 492 NVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQFNIM 551
++P+PV+A+FS RGPN ++P+ILKPD+I PGVNILAAW++++GPTGL +D RKT+FNI+
Sbjct: 488 GIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNIL 547
Query: 552 SGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELSNPWA 611
SGTSM+CPH+SG AALLK+AHP+WSP+ I+SA+MTT +DN+N SL D + G+ + P+
Sbjct: 548 SGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYD 607
Query: 612 HGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCSKKFADPGQL 671
+GSGH++ +A++PGLVYDI+ D+YI FLCS+ YG +Q I + + + PG L
Sbjct: 608 YGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNL 667
Query: 672 NYPSFSVVFGSKR--VVRYT--REVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVG 731
NYPS + VF + R +V T R TNVG A +VY +P V V VKP +LVFT
Sbjct: 668 NYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAV 727
Query: 732 EKQRYTVTFVASRGAAQTMRFG--FGSIAW-SNDQHQVRSPV 759
+++ Y VT + G FGS+ W +H VRSP+
Sbjct: 728 KRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPI 756
BLAST of Sed0019841 vs. ExPASy TrEMBL
Match:
A0A6J1JIF3 (subtilisin-like protease SBT1.8 OS=Cucurbita maxima OX=3661 GN=LOC111484737 PE=3 SV=1)
HSP 1 Score: 1363.6 bits (3528), Expect = 0.0e+00
Identity = 681/766 (88.90%), Postives = 720/766 (93.99%), Query Frame = 0
Query: 1 MDSMARFLILS-LLLLLLLPCAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHS 60
M SMAR L+ S LLLLLLLPC +NAK+TYIV+MKH LPS+Y THHDW+SAHLQSLS S
Sbjct: 1 MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60
Query: 61 STSDSLLYTYTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSN 120
+TSDSLLYTYTSAYHGFAASLDPDE ELL QSDSVLGVYED+VY LHTTRTP FLGLDS+
Sbjct: 61 ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDSD 120
Query: 121 FNLWEGHNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPS 180
F LWEGHNTQDL+QASHDVIIGVLDTGIWP SKSFDD GMPEIP+RWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
Query: 181 LCNKKLIGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLG 240
LCNKKLIGARSF KGY+MASGGGY ++ ENESP+D+DGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
Query: 241 YASGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHRD 300
YA GIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRA+SDGVDVLSLSLGGGSAPY+RD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
Query: 361 FVGVSLYSGEGMGKQPVGLVYSKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKG 420
F GVSLYSG+GMG +PV LVY+KGSNSSSN+CL GSLEP V+GKVV+CDRG+NARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420
Query: 421 GVVRQAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFG 480
GVVR AGG+GMILANTAASGEELVADSHLLPAVAVG TGDLIRQYV S A+PTA LSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480
Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQ 540
GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540
Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELS 600
FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYT DNTNSSLRDAAGG S
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFS 600
Query: 601 NPWAHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCSKKFAD 660
NPWAHG+GHVDPHKALSPGL+YDIST++YI FLCSLDYG+DHVQAIVK SN+TCSKKFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKKFAD 660
Query: 661 PGQLNYPSFSVVFGSKRVVRYTREVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVG 720
PGQLNYPSFSVVFGSKRVVRYTR VTNVGAA SVYEVATTAPSVVKV VKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720
Query: 721 EKQRYTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAWTRL 766
E++RYTVTFVASR AA+T R+GFGSIAWSNDQHQVRSPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766
BLAST of Sed0019841 vs. ExPASy TrEMBL
Match:
A0A6J1H8F1 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111461488 PE=3 SV=1)
HSP 1 Score: 1361.7 bits (3523), Expect = 0.0e+00
Identity = 680/766 (88.77%), Postives = 718/766 (93.73%), Query Frame = 0
Query: 1 MDSMARFLILS-LLLLLLLPCAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHS 60
M SMAR L+ S LLLLLLLPC +NAK+TYIV+MKH LPS+Y THHDW+SAHLQSLS S
Sbjct: 1 MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60
Query: 61 STSDSLLYTYTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSN 120
+TSDSLLYTYTSAYHGFAASLDPDE ELL QSDSVLGVYED+VY LHTTRTP FLGLDS+
Sbjct: 61 ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
Query: 121 FNLWEGHNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPS 180
F LWEGHNTQDL+QASHDVIIGVLDTGIWP SKSFDD GMPEIP+RWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
Query: 181 LCNKKLIGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLG 240
LCNKKLIGARSF KGY+MASGGGY ++ ENESP+D+DGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
Query: 241 YASGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHRD 300
YA GIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRA+SDGVDVLSLSLGGGSAPY+RD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
Query: 361 FVGVSLYSGEGMGKQPVGLVYSKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKG 420
F GVSLYSG+GMG +PV LVY+KGSNSSSN+CL GSLEP V+GKVV+CDRG+NARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420
Query: 421 GVVRQAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFG 480
GVVR AGG+GMILANTAASGEELVADSHLLPAVAVG TGDLIRQYV S A+PTA LSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480
Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQ 540
GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540
Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELS 600
FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYT DNTNSSLRDAAGG S
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
Query: 601 NPWAHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCSKKFAD 660
NPWAHG+GHVDPHKALSPGLVYDIST++YI FLCSLDYG+DHVQAI K SN+TC KKFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660
Query: 661 PGQLNYPSFSVVFGSKRVVRYTREVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVG 720
PGQLNYPSFSVVFGSKRVVRYTR VTNVGAA SVYEVATTAPSVVKV VKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720
Query: 721 EKQRYTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAWTRL 766
E++RYTVTFVASR AA+T R+GFGSIAWSNDQHQVRSPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766
BLAST of Sed0019841 vs. ExPASy TrEMBL
Match:
A0A5A7UKV3 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001560 PE=3 SV=1)
HSP 1 Score: 1341.3 bits (3470), Expect = 0.0e+00
Identity = 671/765 (87.71%), Postives = 712/765 (93.07%), Query Frame = 0
Query: 1 MDSMARFLILSLLLLLLLPCAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHSS 60
MDSM RFLI LLLLLLL C INAKKTYIV MKH LPSEY THHDW+SA LQSLS SS
Sbjct: 1 MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60
Query: 61 TSDSLLYTYTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSNF 120
+SDSLLYTYTS++HGFAA LD +EVELL QSDSVLGVYED+VY LHTTRTP FLGLDS+F
Sbjct: 61 SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
Query: 121 NLWEGHNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPSL 180
LWEGH TQDLNQASHDVIIGVLDTGIWP SKSFDD GMPEIP+RWRG CE+GPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180
Query: 181 CNKKLIGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLGY 240
CNKKLIGARSF KGY+MASGGGY + EN+S +D+DGHGTHTASTAAGSHV NASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240
Query: 241 ASGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHRDT 300
A GIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRA+SDGVDVLSLSLGGGSAPY+RDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
Query: 361 VGVSLYSGEGMGKQPVGLVYSKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKGG 420
GVSLYSG+GMG + V LVY+KGSN+SSN+CL GSL+P +V+GKVVVCDRG+NARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420
Query: 421 VVRQAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFGG 480
VVR AGG+GMILANTAASGEELVADSHLLPAVAVGR TGDLIRQYV S + PTA LSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480
Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQF 540
T+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL+ DKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540
Query: 541 NIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELSN 600
NIMSGTSMSCPHISGLAALLKAAHP+WSPSAIKSALMTTAYT DNTNSSLRDAAGG SN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600
Query: 601 PWAHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCSKKFADP 660
PWAHG+GHVDPHKALSPGL+YDIST++YIAFLCSLDYG+DHVQAIVK SN+TCS+KFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660
Query: 661 GQLNYPSFSVVFGSKRVVRYTREVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVGE 720
GQLNYPSFSVVFGSKRVVRYTR VTNVGAA SVY+VATTAPSVVKV VKPSKLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720
Query: 721 KQRYTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAWTRL 766
++RYTVTFVASR AAQT RFGFGSI WSNDQHQVRSPV+FAWTRL
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765
BLAST of Sed0019841 vs. ExPASy TrEMBL
Match:
A0A1S3BQE8 (subtilisin-like protease SBT1.8 OS=Cucumis melo OX=3656 GN=LOC103492374 PE=3 SV=1)
HSP 1 Score: 1341.3 bits (3470), Expect = 0.0e+00
Identity = 671/765 (87.71%), Postives = 712/765 (93.07%), Query Frame = 0
Query: 1 MDSMARFLILSLLLLLLLPCAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHSS 60
MDSM RFLI LLLLLLL C INAKKTYIV MKH LPSEY THHDW+SA LQSLS SS
Sbjct: 1 MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60
Query: 61 TSDSLLYTYTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSNF 120
+SDSLLYTYTS++HGFAA LD +EVELL QSDSVLGVYED+VY LHTTRTP FLGLDS+F
Sbjct: 61 SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
Query: 121 NLWEGHNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPSL 180
LWEGH TQDLNQASHDVIIGVLDTGIWP SKSFDD GMPEIP+RWRG CE+GPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180
Query: 181 CNKKLIGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLGY 240
CNKKLIGARSF KGY+MASGGGY + EN+S +D+DGHGTHTASTAAGSHV NASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240
Query: 241 ASGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHRDT 300
A GIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRA+SDGVDVLSLSLGGGSAPY+RDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
Query: 361 VGVSLYSGEGMGKQPVGLVYSKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKGG 420
GVSLYSG+GMG + V LVY+KGSN+SSN+CL GSL+P +V+GKVVVCDRG+NARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420
Query: 421 VVRQAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFGG 480
VVR AGG+GMILANTAASGEELVADSHLLPAVAVGR TGDLIRQYV S + PTA LSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480
Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQF 540
T+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL+ DKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540
Query: 541 NIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELSN 600
NIMSGTSMSCPHISGLAALLKAAHP+WSPSAIKSALMTTAYT DNTNSSLRDAAGG SN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600
Query: 601 PWAHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCSKKFADP 660
PWAHG+GHVDPHKALSPGL+YDIST++YIAFLCSLDYG+DHVQAIVK SN+TCS+KFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660
Query: 661 GQLNYPSFSVVFGSKRVVRYTREVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVGE 720
GQLNYPSFSVVFGSKRVVRYTR VTNVGAA SVY+VATTAPSVVKV VKPSKLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720
Query: 721 KQRYTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAWTRL 766
++RYTVTFVASR AAQT RFGFGSI WSNDQHQVRSPV+FAWTRL
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765
BLAST of Sed0019841 vs. ExPASy TrEMBL
Match:
A0A5D3CDV9 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001530 PE=3 SV=1)
HSP 1 Score: 1336.2 bits (3457), Expect = 0.0e+00
Identity = 669/763 (87.68%), Postives = 710/763 (93.05%), Query Frame = 0
Query: 1 MDSMARFLILSLLLLLLLPCAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHSS 60
MDSM RFLI LLLLLL C INAKKTYIV MKH LPSEY THHDW+SA LQSLS SS
Sbjct: 1 MDSMPRFLI-PFLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60
Query: 61 TSDSLLYTYTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSNF 120
+SDSLLYTYTS++HGFAA LD +EVELL QSDSVLGVYED+VY LHTTRTP FLGLDS+F
Sbjct: 61 SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
Query: 121 NLWEGHNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPSL 180
LWEGH TQDLNQASHDVIIGVLDTGIWP SKSFDD GMPEIP+RWRGECE+GPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECEAGPDFSPSL 180
Query: 181 CNKKLIGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLGY 240
CNKKLIGARSF KGY+MASGGGY + EN+S +D+DGHGTHTASTAAGSHV NASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240
Query: 241 ASGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHRDT 300
A GIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRA+SDGVDVLSLSLGGGSAPY+RDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
Query: 361 VGVSLYSGEGMGKQPVGLVYSKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKGG 420
GVSLYSG+GMG + V LVY+KGSN+SSN+CL GSL+P +V+GKVVVCDRG+NARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420
Query: 421 VVRQAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFGG 480
VVR AGG+GMILANTAASGEELVADSHLLPAVAVGR TGDLIRQYV S + PTA LSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480
Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQF 540
T+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL+ DKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540
Query: 541 NIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELSN 600
NIMSGTSMSCPHISGLAALLKAAHP+WSPSAIKSALMTTAYT DNTNSSLRDAAGG SN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600
Query: 601 PWAHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCSKKFADP 660
PWAHG+GHVDPHKALSPGL+YDIST++YIAFLCSLDYG+DHVQAIVK SN+TCS+KFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660
Query: 661 GQLNYPSFSVVFGSKRVVRYTREVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVGE 720
GQLNYPSFSVVFGSKRVVRYTR VTNVGAA SVY+VATTAPSVVKV VKPSKLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720
Query: 721 KQRYTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAWT 764
++RYTVTFVASR AAQT RFGFGSI WSNDQHQVRSPV+FAWT
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWT 762
BLAST of Sed0019841 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 544/763 (71.30%), Postives = 633/763 (82.96%), Query Frame = 0
Query: 3 SMARFLILSLLLLLLLPCAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHSSTS 62
S + I++ L LLL AKKTYI+++ HS P + THHDW+++ L S S
Sbjct: 7 SSSSITIITTFLFLLLH---TTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSES----- 66
Query: 63 DSLLYTYTSAYHGFAASLDPDEVE-LLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSNFN 122
SLLYTYT+++HGF+A LD E + LLS S+S+L ++ED +YTLHTTRTPEFLGL+S F
Sbjct: 67 -SLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFG 126
Query: 123 LWEGHNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPSLC 182
+ DL +S+ VIIGVLDTG+WP S+SFDD MPEIP++W+GECESG DF LC
Sbjct: 127 V------HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLC 186
Query: 183 NKKLIGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLGYA 242
NKKLIGARSF KG++MASGGG+ + E+ SP+D DGHGTHT++TAAGS V NAS LGYA
Sbjct: 187 NKKLIGARSFSKGFQMASGGGFSSKR-ESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYA 246
Query: 243 SGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHRDTI 302
+G ARGMA +ARVA YK CW TGCFGSDILA MDRA+ DGVDVLSLSLGGGSAPY+RDTI
Sbjct: 247 AGTARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTI 306
Query: 303 AIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFV 362
AIGAF+AME+GVFVSCSAGNSGP +AS+ANVAPW+MTVGAGTLDRDFPA+ LGNGKR
Sbjct: 307 AIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLT 366
Query: 363 GVSLYSGEGMGKQPVGLVYSKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKGGV 422
GVSLYSG GMG +P+ LVY+KG++SSSNLCL GSL+ +V+GK+VVCDRGVNARVEKG V
Sbjct: 367 GVSLYSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAV 426
Query: 423 VRQAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFGGT 482
VR AGG+GMI+ANTAASGEELVADSHLLPA+AVG+ TGDL+R+YV+S ++PTA L F GT
Sbjct: 427 VRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGT 486
Query: 483 VLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQFN 542
VL+V+PSPVVAAFSSRGPN VTP+ILKPDVIGPGVNILA WS++IGPTGLD D R+TQFN
Sbjct: 487 VLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFN 546
Query: 543 IMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELSNP 602
IMSGTSMSCPHISGLA LLKAAHPEWSPSAIKSALMTTAY LDNTN+ L DAA LSNP
Sbjct: 547 IMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNP 606
Query: 603 WAHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCSKKFADPG 662
+AHGSGHVDP KALSPGLVYDIST+EYI FLCSLDY +DH+ AIVK +V CSKKF+DPG
Sbjct: 607 YAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPG 666
Query: 663 QLNYPSFSVVFGSKRVVRYTREVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVGEK 722
QLNYPSFSV+FG KRVVRYTREVTNVGAA+SVY+V V + VKPSKL F VGEK
Sbjct: 667 QLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEK 726
Query: 723 QRYTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAWTR 765
+RYTVTFV+ +G + T + FGSI WSN QH+VRSPVAF+W R
Sbjct: 727 KRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNR 753
BLAST of Sed0019841 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 786.6 bits (2030), Expect = 1.8e-227
Identity = 415/760 (54.61%), Postives = 534/760 (70.26%), Query Frame = 0
Query: 13 LLLLLLPCAIINAKK---TYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHSSTSDSLLYTY 72
LLL L C + ++ TYIV M S +PS + H +W+ + L+S+S S+ LLYTY
Sbjct: 13 LLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA---ELLYTY 72
Query: 73 TSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSNFNLWEGHNTQ 132
+A HGF+ L +E + L V+ V + Y LHTTRTP FLGLD +T
Sbjct: 73 ENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD--------EHTA 132
Query: 133 DLNQAS---HDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPSLCNKKLI 192
DL + DV++GVLDTG+WP SKS+ D G IP+ W+G CE+G +F+ SLCN+KLI
Sbjct: 133 DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLI 192
Query: 193 GARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLGYASGIAR 252
GAR F +GY S G + + E+ SP+D+DGHGTHT+STAAGS V ASLLGYASG AR
Sbjct: 193 GARFFARGYE--STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTAR 252
Query: 253 GMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHRDTIAIGAF 312
GMAP+ARVA YK CW GCF SDILA +D+A++D V+VLS+SLGGG + Y+RD +AIGAF
Sbjct: 253 GMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAF 312
Query: 313 AAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFVGVSLY 372
AAME+G+ VSCSAGN+GP+ +SL+NVAPWI TVGAGTLDRDFPA LGNGK F GVSL+
Sbjct: 313 AAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLF 372
Query: 373 SGEGMGKQPVGLVYSKGSNSSS--NLCLVGSLEPGMVKGKVVVCDRGVNARVEKGGVVRQ 432
GE + + + +Y+ +++++ NLC+ G+L P VKGK+V+CDRG+NARV+KG VV+
Sbjct: 373 KGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKA 432
Query: 433 AGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFGGTVLN 492
AGGVGMILANTAA+GEELVAD+HLLPA VG GD+IR YV + PTA++S GTV+
Sbjct: 433 AGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG 492
Query: 493 VRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQFNIMS 552
V+PSPVVAAFSSRGPN +TP ILKPD+I PGVNILAAW+ + GPTGL +D R+ +FNI+S
Sbjct: 493 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIIS 552
Query: 553 GTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELSNPWAH 612
GTSMSCPH+SGLAALLK+ HPEWSP+AI+SALMTTAY L D A G+ S P+ H
Sbjct: 553 GTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDH 612
Query: 613 GSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCS-KKFADPGQL 672
G+GHV P A +PGL+YD++T++Y+ FLC+L+Y +++ V N TC K L
Sbjct: 613 GAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRS-VSRRNYTCDPSKSYSVADL 672
Query: 673 NYPSFSVVFGSKRVVRYTREVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVGEKQR 732
NYPSF+V +YTR VT+VG A + T+ + VK+ V+P+ L F + EK+
Sbjct: 673 NYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKS 732
Query: 733 YTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAWT 764
YTVTF FGSI WS+ +H V SPVA +WT
Sbjct: 733 YTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISWT 757
BLAST of Sed0019841 vs. TAIR 10
Match:
AT3G14067.1 (Subtilase family protein )
HSP 1 Score: 754.6 bits (1947), Expect = 7.8e-218
Identity = 410/768 (53.39%), Postives = 515/768 (67.06%), Query Frame = 0
Query: 7 FLILSLLLLLLLP-CAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHSSTSDSL 66
F + LLL P + + ++YIV ++ S PS + +H++W + L+SL S +L
Sbjct: 10 FFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATL 69
Query: 67 LYTYTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSNFNLWEG 126
LY+Y+ A HGF+A L P + L + SV+ V D +HTT TP FLG N LW
Sbjct: 70 LYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSN 129
Query: 127 HNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPSLCNKKL 186
N DVI+GVLDTGIWP SF D+G+ IP+ W+GECE GPDF S CN+KL
Sbjct: 130 SN------YGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKL 189
Query: 187 IGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLGYASGIA 246
IGAR+F++GY G A E+ SP+D +GHGTHTASTAAGS VANASL YA G A
Sbjct: 190 IGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTA 249
Query: 247 RGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLG-GGSAP-YHRDTIAI 306
GMA +AR+AAYK CW GC+ SDILA MD+A++DGV V+SLS+G GSAP YH D+IAI
Sbjct: 250 TGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAI 309
Query: 307 GAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFVGV 366
GAF A G+ VSCSAGNSGPN + N+APWI+TVGA T+DR+F A G+GK F G
Sbjct: 310 GAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGT 369
Query: 367 SLYSGEGMGKQPVGLVYSKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKGGVVR 426
SLY+GE + + LVYS + S LC G L +V+GK+V+CDRG NARVEKG V+
Sbjct: 370 SLYAGESLPDSQLSLVYS--GDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVK 429
Query: 427 QAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFGGTVL 486
AGG GMILANTA SGEEL ADSHL+PA VG GD IR Y++++ PTA +SF GT++
Sbjct: 430 LAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLI 489
Query: 487 NVR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQFNI 546
PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+ +GPT LD D R+ QFNI
Sbjct: 490 GPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNI 549
Query: 547 MSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELSNPW 606
+SGTSMSCPH+SGLAALL+ AHP+WSP+AIKSAL+TTAY ++N+ + D A G+ SN +
Sbjct: 550 ISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSF 609
Query: 607 AHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNV--TC-SKKFAD 666
HG+GHVDP+KAL+PGLVYDI EY+AFLC++ Y + ++ + C + K
Sbjct: 610 IHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRT 669
Query: 667 PGQLNYPSFSVVFGSK-RVVRYTREVTNVGA-AASVYEVATTAPSVVKVVVKPSKLVFTK 726
G LNYPSFSVVF S VV+Y R V NVG+ +VYEV +P+ V++ V PSKL F+K
Sbjct: 670 AGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSK 729
Query: 727 VGEKQRYTVTF---VASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAW 763
Y VTF V G FGSI W++ +H V+SPVA W
Sbjct: 730 EKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769
BLAST of Sed0019841 vs. TAIR 10
Match:
AT5G51750.1 (subtilase 1.3 )
HSP 1 Score: 735.7 bits (1898), Expect = 3.7e-212
Identity = 393/774 (50.78%), Postives = 516/774 (66.67%), Query Frame = 0
Query: 7 FLILSLLLLLLL--PCAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLS-HSS--- 66
F+ILS+ L+ L I+ KKTY++ M S +P Y H W+S+ + S++ H S
Sbjct: 13 FIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEE 72
Query: 67 --TSDSLLYTYTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDS 126
++ +LYTY +A+HG AA L +E E L + D V+ V ++ Y LHTTR+P FLGL+
Sbjct: 73 EGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLER 132
Query: 127 NFNLWEGHNTQDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSP 186
E HDV++GVLDTGIWP S+SF+D GM +PA WRG CE+G F
Sbjct: 133 Q----ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLK 192
Query: 187 SLCNKKLIGARSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLL 246
CN+K++GAR F++GY A+ G + E +SP+D DGHGTHTA+T AGS V A+L
Sbjct: 193 RNCNRKIVGARVFYRGYEAAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLF 252
Query: 247 GYASGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRALSDGVDVLSLSLGGGSAPYHR 306
G+A G ARGMA +ARVAAYK CW GCF SDIL+ +D+A++DGV VLS+SLGGG + Y R
Sbjct: 253 GFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSR 312
Query: 307 DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGK 366
D+++I F AME GVFVSCSAGN GP+ SL NV+PWI TVGA T+DRDFPA V++G +
Sbjct: 313 DSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMR 372
Query: 367 RFVGVSLYSGEGM--GKQPVGLVYSKGSNSSS----NLCLVGSLEPGMVKGKVVVCDRGV 426
F GVSLY G + + LVY G N+SS + CL G+L+ V GK+V+CDRGV
Sbjct: 373 TFKGVSLYKGRTVLPKNKQYPLVY-LGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGV 432
Query: 427 NARVEKGGVVRQAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEP 486
RV+KG VV++AGG+GM+L NTA +GEELVADSH+LPAVAVG G LI+QY ++ +
Sbjct: 433 TPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKA 492
Query: 487 TAALSFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD 546
TA+L GT + ++PSPVVAAFSSRGPN ++ +ILKPD++ PGVNILAAW+ + P+ L
Sbjct: 493 TASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLS 552
Query: 547 TDKRKTQFNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRD 606
+D R+ +FNI+SGTSMSCPH+SG+AAL+K+ HP+WSP+AIKSALMTTAY DN L D
Sbjct: 553 SDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTD 612
Query: 607 AAGGELSNPWAHGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVT 666
A+G S+P+ HG+GH+DP +A PGLVYDI EY FLC+ D ++ KHSN T
Sbjct: 613 ASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRT 672
Query: 667 CSKKFA-DPGQLNYPSFSVVFGSK---RVVRYTREVTNVGAAASVYEVATTAPSVVKVVV 726
C A +PG LNYP+ S +F + + R VTNVG S Y+V+ + V V
Sbjct: 673 CKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTV 732
Query: 727 KPSKLVFTKVGEKQRYTVTFVASRGAAQTMRFGFGSIAWSNDQHQVRSPVAFAW 763
+P L FT +K YTVTF R + R FG + W + H+VRSPV W
Sbjct: 733 QPKTLNFTSKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776
BLAST of Sed0019841 vs. TAIR 10
Match:
AT4G34980.1 (subtilisin-like serine protease 2 )
HSP 1 Score: 715.7 bits (1846), Expect = 4.0e-206
Identity = 381/762 (50.00%), Postives = 513/762 (67.32%), Query Frame = 0
Query: 12 LLLLLLLP---CAIINAKKTYIVQMKHSPLPSEYPTHHDWFSAHLQSLSHSSTSDSLLYT 71
LLL L P A A KT+I ++ +PS +PTH+ W+S S +++
Sbjct: 8 LLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESR------IVHV 67
Query: 72 YTSAYHGFAASLDPDEVELLSQSDSVLGVYEDSVYTLHTTRTPEFLGLDSNFNLWEGHNT 131
Y + +HGF+A + PDE + L +VL V+ED LHTTR+P+FLGL + LW
Sbjct: 68 YHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSE--- 127
Query: 132 QDLNQASHDVIIGVLDTGIWPGSKSFDDAGMPEIPARWRGECESGPDFSPSLCNKKLIGA 191
+ DVIIGV DTGIWP +SF D + IP RWRG CESG FSP CN+K+IGA
Sbjct: 128 ---SDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGA 187
Query: 192 RSFWKGYRMASGGGYLGRAGENESPKDEDGHGTHTASTAAGSHVANASLLGYASGIARGM 251
R F KG + A GG + + E SP+D DGHGTHT+STAAG H AS+ GYASG+A+G+
Sbjct: 188 RFFAKGQQAAVIGG-INKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGV 247
Query: 252 APQARVAAYKTCW-PTGCFGSDILAGMDRALSDGVDVLSLSLGGG---SAPYHRDTIAIG 311
AP+AR+AAYK CW +GC SDILA D A+ DGVDV+S+S+GGG ++PY+ D IAIG
Sbjct: 248 APKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIG 307
Query: 312 AFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFVGVS 371
++ A KG+FVS SAGN GPN S+ N+APW+ TVGA T+DR+FPA LG+G R GVS
Sbjct: 308 SYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVS 367
Query: 372 LYSGEGMGKQPVGLVY-SKGSNSSSNLCLVGSLEPGMVKGKVVVCDRGVNARVEKGGVVR 431
LY+G + + +VY K SS++LC+ +L+P V+GK+V+CDRG + RV KG VV+
Sbjct: 368 LYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVK 427
Query: 432 QAGGVGMILANTAASGEELVADSHLLPAVAVGRNTGDLIRQYVESAAEPTAALSFGGTVL 491
+AGGVGMILAN A++GE LV D+HL+PA AVG N GD I+ Y S P A++ F GT++
Sbjct: 428 KAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIV 487
Query: 492 NVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDTDKRKTQFNIM 551
++P+PV+A+FS RGPN ++P+ILKPD+I PGVNILAAW++++GPTGL +D RKT+FNI+
Sbjct: 488 GIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNIL 547
Query: 552 SGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTLDNTNSSLRDAAGGELSNPWA 611
SGTSM+CPH+SG AALLK+AHP+WSP+ I+SA+MTT +DN+N SL D + G+ + P+
Sbjct: 548 SGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYD 607
Query: 612 HGSGHVDPHKALSPGLVYDISTDEYIAFLCSLDYGLDHVQAIVKHSNVTCSKKFADPGQL 671
+GSGH++ +A++PGLVYDI+ D+YI FLCS+ YG +Q I + + + PG L
Sbjct: 608 YGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNL 667
Query: 672 NYPSFSVVFGSKR--VVRYT--REVTNVGAAASVYEVATTAPSVVKVVVKPSKLVFTKVG 731
NYPS + VF + R +V T R TNVG A +VY +P V V VKP +LVFT
Sbjct: 668 NYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAV 727
Query: 732 EKQRYTVTFVASRGAAQTMRFG--FGSIAW-SNDQHQVRSPV 759
+++ Y VT + G FGS+ W +H VRSP+
Sbjct: 728 KRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPI 756
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| KAG6590040.1 | 0.0e+00 | 88.76 | Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| XP_022987083.1 | 0.0e+00 | 88.90 | subtilisin-like protease SBT1.8 [Cucurbita maxima] | [more] |
| XP_023516159.1 | 0.0e+00 | 88.63 | subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo] | [more] |
| XP_022960792.1 | 0.0e+00 | 88.77 | subtilisin-like protease SBT1.8 [Cucurbita moschata] >KAG7023704.1 Subtilisin-li... | [more] |
| XP_038879224.1 | 0.0e+00 | 89.15 | subtilisin-like protease SBT1.8 [Benincasa hispida] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9ZUF6 | 0.0e+00 | 71.30 | Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... | [more] |
| O65351 | 2.6e-226 | 54.61 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| Q9LVJ1 | 1.1e-216 | 53.39 | Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 S... | [more] |
| Q9FLI4 | 5.2e-211 | 50.78 | Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... | [more] |
| O49607 | 5.6e-205 | 50.00 | Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1JIF3 | 0.0e+00 | 88.90 | subtilisin-like protease SBT1.8 OS=Cucurbita maxima OX=3661 GN=LOC111484737 PE=3... | [more] |
| A0A6J1H8F1 | 0.0e+00 | 88.77 | subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111461488 PE... | [more] |
| A0A5A7UKV3 | 0.0e+00 | 87.71 | Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... | [more] |
| A0A1S3BQE8 | 0.0e+00 | 87.71 | subtilisin-like protease SBT1.8 OS=Cucumis melo OX=3656 GN=LOC103492374 PE=3 SV=... | [more] |
| A0A5D3CDV9 | 0.0e+00 | 87.68 | Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... | [more] |
