Sed0019774 (gene) Chayote v1

BLAST of Sed0019774 vs. NCBI nr
Match: XP_023007495.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1499.6 bits (3881), Expect = 0.0e+00
Identity = 780/946 (82.45%), Postives = 849/946 (89.75%), Query Frame = 0

Query: 1   MEDSGN---------NTTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTCP 60
           ME+SGN         NT PKDFVCPITCNIFYDPVTIETGQTYER+AIQEWLDRGNSTCP
Sbjct: 1   MEESGNNGLESGEMHNTHPKDFVCPITCNIFYDPVTIETGQTYERSAIQEWLDRGNSTCP 60

Query: 61  ITGQKLQNTQLPKTNYVLKRLIASWLEENPTFVSEKPFG---LTSPVNV-----INRTME 120
           ITGQKLQNTQLPKTNYVLKRLIASWLEENP FV++K      LTSPV+V     INR+ME
Sbjct: 61  ITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVTDKADSAAVLTSPVSVISQASINRSME 120

Query: 121 VRQAITNLYLSEVLDDAEAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILVNSVNL 180
           VR AI+N+  SEVL++AE+AVLCVERFWLEENV+++IQ+MLLKPPVINGLVEIL+NSV+L
Sbjct: 121 VRHAISNIVSSEVLEEAESAVLCVERFWLEENVDVEIQHMLLKPPVINGLVEILINSVDL 180

Query: 181 HVLRASIFLLSELGFKDAAVIQMLTRVESDVDCILTLFKSGLMEAVVLIYQFGLSVQHLQ 240
             LRA+IFLLSELGFKDAAVIQMLTRVESDVDCI+TLFKSGLMEAVVLIY+FGLS+Q LQ
Sbjct: 181 QALRATIFLLSELGFKDAAVIQMLTRVESDVDCIVTLFKSGLMEAVVLIYRFGLSIQCLQ 240

Query: 241 EMDLAGFLLNVVKKKGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAENAIDS 300
           EMDLAG LLNVVKKK +DVN KM+LS KSAAVILLRKILGRSKEGS+IAVAVLAENAI+S
Sbjct: 241 EMDLAGSLLNVVKKK-EDVN-KMQLSPKSAAVILLRKILGRSKEGSMIAVAVLAENAIES 300

Query: 301 ILGSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADEAHAHLASILGSFMEVSNEEQFEI 360
           IL SLKAKQVEERIAAVGILLRCIQEDGKCRN+IAD+  A LA ILGSFMEVSN EQFEI
Sbjct: 301 ILVSLKAKQVEERIAAVGILLRCIQEDGKCRNMIADK--ADLAPILGSFMEVSNGEQFEI 360

Query: 361 VMFLSDLVKLNRRTFNQQILQSIRDGGGCSTMHSLLIYLQTAHKDQSPVVAGLLLQLDIL 420
           +MFLS+LVKLNRRTFN+QILQ+I+DGG CSTMHSLL+YLQTA KDQ PVVAG LLQLDIL
Sbjct: 361 IMFLSELVKLNRRTFNEQILQNIKDGGECSTMHSLLVYLQTAPKDQCPVVAGFLLQLDIL 420

Query: 421 VEPRKMSIYREEAMDVLFSCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV 480
           VEPRK SIYREEAMDVL SCLG+SDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV
Sbjct: 421 VEPRKSSIYREEAMDVLLSCLGNSDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV 480

Query: 481 GFMIGRMKLKPRGNNSNAPGQVKLTTEEERANDEWERKMAFVLMSHDFGLVFEPLAKGLK 540
           GF  G MK K R NNS+ PG V+L+  EER+NDEWERKMAFVL SH+FGL+FEPLAKGLK
Sbjct: 481 GFAKGCMKPKRRDNNSSGPGDVELSIAEERSNDEWERKMAFVLASHNFGLLFEPLAKGLK 540

Query: 541 SKYAALFSACFVLATWLCHMLRVLPDTGILEAARVCLLDHFISIFTTTTDIEEKALGLLA 600
           SKYAALFSACFV ATWL HML+VLPDTGIL+AARVCLLDHF+SIF TTTDIEEKAL LL 
Sbjct: 541 SKYAALFSACFVSATWLSHMLKVLPDTGILDAARVCLLDHFVSIFVTTTDIEEKALALLG 600

Query: 601 MNSFVHEPEGMQCLRSNMKSINSGLKELRRSTPLAFEMLKVLCEGEESSTELWNHQELFQ 660
           MNSFVH+PEG+Q L SN K I  GLKELRRST LAFEMLKVLCEGEESS ELW+HQELF 
Sbjct: 601 MNSFVHQPEGLQYLSSNTKDIMRGLKELRRSTALAFEMLKVLCEGEESSAELWSHQELFL 660

Query: 661 VDCSKNGEVLSIVYFKDKTISGHSDGTIKVWSVRGTNIHLLQETQEHSKGVTSLAITESD 720
           VDCSKNGEVLSI Y K++ ISGHSDGTIKVWSV GTN+HLLQETQEHSKGVTSLAI ES+
Sbjct: 661 VDCSKNGEVLSIAYLKEQIISGHSDGTIKVWSVGGTNLHLLQETQEHSKGVTSLAIIESE 720

Query: 721 DKLYSGSLDKTIKVWSLDSDKIQCIQVHDVKDQIHNLVVSKTVACFVPHGAGIRVYSWGG 780
           +KLYSGSLDKTIKVWSL SD IQCI+VHDVKDQ+HNLVVSKT+ACF+PHGAGIR YSWGG
Sbjct: 721 EKLYSGSLDKTIKVWSLGSDVIQCIKVHDVKDQVHNLVVSKTIACFIPHGAGIRGYSWGG 780

Query: 781 ESKLLNSSKHVKCLDLVHGKLYCGCHDGSIQEVDLATETLSNIHSGSRKLLGKAPSVQAL 840
           ESKLLNS+KHVKCL+LV GKLYCGCHD SIQEVDLAT T+S IH GSRKLLGK   VQ+L
Sbjct: 781 ESKLLNSNKHVKCLNLVRGKLYCGCHDSSIQEVDLATGTVSYIHIGSRKLLGKPNIVQSL 840

Query: 841 QIYDEQLFSACTTLDGSAVKIWNTSNNNVIASLSTPMDVRSMIVSSDLIYVGTR-GVVEM 900
           QIY+EQLFSA TTLDG+AVKIW+ SNN+VIASLST MD+R+M VSSDL Y+G R GVVE+
Sbjct: 841 QIYEEQLFSASTTLDGAAVKIWSMSNNSVIASLSTAMDIRTMAVSSDLTYLGGRGGVVEI 900

Query: 901 WSREKYNKIDTLQTGRNCKVACMALSEREDVLVIGTSDGRIQAWGL 929
           W R+K NKIDTLQTGR CKVACM L+EREDVLVIGTSDGRIQ WGL
Sbjct: 901 WGRDKLNKIDTLQTGRICKVACMTLNEREDVLVIGTSDGRIQGWGL 942

BLAST of Sed0019774 vs. NCBI nr
Match: KAG6587976.1 (putative E3 ubiquitin-protein ligase LIN-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1498.0 bits (3877), Expect = 0.0e+00
Identity = 781/952 (82.04%), Postives = 849/952 (89.18%), Query Frame = 0

Query: 1   MEDSGN----------NTTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTC 60
           ME+SGN          NT PKDFVCPITCNIFYDPVTIETGQTYER+AIQEWLDRGNSTC
Sbjct: 1   MEESGNNGLESGEMHHNTHPKDFVCPITCNIFYDPVTIETGQTYERSAIQEWLDRGNSTC 60

Query: 61  PITGQKLQNTQLPKTNYVLKRLIASWLEENPTFVSEKPFG-------LTSPVNV-----I 120
           PITGQKLQNTQLPKTNYVLKRLIASWLEENP FV++K          LTSPV+V     I
Sbjct: 61  PITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVTDKALDEADSAAVLTSPVSVISQASI 120

Query: 121 NRTMEVRQAITNLYLSEVLDDAEAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILV 180
           NR+MEVR AI+N+  SEVL++AE+AVLCVERFWLEENV+++IQ+MLLKPPVINGLVEIL+
Sbjct: 121 NRSMEVRHAISNIVSSEVLEEAESAVLCVERFWLEENVDVEIQHMLLKPPVINGLVEILI 180

Query: 181 NSVNLHVLRASIFLLSELGFKDAAVIQMLTRVESDVDCILTLFKSGLMEAVVLIYQFGLS 240
           NSV+L VLRA+IFLLSELGFKDAAVIQ LTRVESDVDCI+TLFKSGLMEAVVL+Y+FGLS
Sbjct: 181 NSVDLQVLRATIFLLSELGFKDAAVIQTLTRVESDVDCIVTLFKSGLMEAVVLMYRFGLS 240

Query: 241 VQHLQEMDLAGFLLNVVKKKGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAE 300
           +Q LQEMDLAG LLNVVKKK +DVN KM+LS KSAAVILLRKILGRSKEGSLIAVAVLAE
Sbjct: 241 IQRLQEMDLAGSLLNVVKKK-EDVN-KMQLSPKSAAVILLRKILGRSKEGSLIAVAVLAE 300

Query: 301 NAIDSILGSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADEAHAHLASILGSFMEVSNE 360
           NAI+SIL SLKAKQVEERIAAVGILLRCIQEDGKCRN+IAD+  A LA ILGSFMEVSN+
Sbjct: 301 NAIESILVSLKAKQVEERIAAVGILLRCIQEDGKCRNMIADK--ADLAPILGSFMEVSND 360

Query: 361 EQFEIVMFLSDLVKLNRRTFNQQILQSIRDGGGCSTMHSLLIYLQTAHKDQSPVVAGLLL 420
           EQFEI+MFLS+LVKLNRRTFN+QILQ I+DGG CSTMHSLL+YLQTA +DQ PVVAG LL
Sbjct: 361 EQFEIIMFLSELVKLNRRTFNEQILQDIKDGGECSTMHSLLVYLQTAPRDQCPVVAGFLL 420

Query: 421 QLDILVEPRKMSIYREEAMDVLFSCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYV 480
           QLDILVEPRK SIYREEAMDVL SCLG+SDFPTAQISAAETIMSLQGRFSTSGRPLARYV
Sbjct: 421 QLDILVEPRKSSIYREEAMDVLLSCLGNSDFPTAQISAAETIMSLQGRFSTSGRPLARYV 480

Query: 481 LLERVGFMIGRMKLKPRGNNSNAPGQVKLTTE-EERANDEWERKMAFVLMSHDFGLVFEP 540
           LLERVGF  G MK K R NNS+ PG V+L+ + EERANDEWERKMAFVL SHDFGL+FEP
Sbjct: 481 LLERVGFAKGCMKPKRRDNNSSGPGDVELSIKAEERANDEWERKMAFVLASHDFGLLFEP 540

Query: 541 LAKGLKSKYAALFSACFVLATWLCHMLRVLPDTGILEAARVCLLDHFISIFTTTTDIEEK 600
           LAKGLKSKYAALFSACFV ATWL HML+VLPDTGIL+AARVCLLDHF+SIF TTTDIEEK
Sbjct: 541 LAKGLKSKYAALFSACFVSATWLSHMLKVLPDTGILDAARVCLLDHFVSIFITTTDIEEK 600

Query: 601 ALGLLAMNSFVHEPEGMQCLRSNMKSINSGLKELRRSTPLAFEMLKVLCEGEESSTELWN 660
           AL LL MNSFVH+PEG+Q L SN K I  GLKELRRST LAFEMLKVLCEGEESS ELW+
Sbjct: 601 ALALLGMNSFVHQPEGLQYLSSNTKDIMRGLKELRRSTALAFEMLKVLCEGEESSAELWS 660

Query: 661 HQELFQVDCSKNGEVLSIVYFKDKTISGHSDGTIKVWSVRGTNIHLLQETQEHSKGVTSL 720
           HQELF VDCS+NGEVLSI YFKDK ISGHSDGTIKVWSV GTN+H LQETQEHSKGVTSL
Sbjct: 661 HQELFLVDCSRNGEVLSIAYFKDKIISGHSDGTIKVWSVGGTNLHPLQETQEHSKGVTSL 720

Query: 721 AITESDDKLYSGSLDKTIKVWSLDSDKIQCIQVHDVKDQIHNLVVSKTVACFVPHGAGIR 780
           AI ES++KLYSGSLDKTIKVWSL SD IQCIQVHDVKDQ+HNLVVSKT+ACF+PHGAGIR
Sbjct: 721 AIIESEEKLYSGSLDKTIKVWSLGSDVIQCIQVHDVKDQVHNLVVSKTIACFIPHGAGIR 780

Query: 781 VYSWGGESKLLNSSKHVKCLDLVHGKLYCGCHDGSIQEVDLATETLSNIHSGSRKLLGKA 840
           VYSWGGESKLLNS+KHVKCL+LV GKLYCGCHD SIQEVDLAT T+S IH GSRKLLGK 
Sbjct: 781 VYSWGGESKLLNSNKHVKCLNLVRGKLYCGCHDSSIQEVDLATGTVSYIHIGSRKLLGKP 840

Query: 841 PSVQALQIYDEQLFSACTTLDGSAVKIWNTSNNNVIASLSTPMDVRSMIVSSDLIYVGTR 900
             VQ+LQIY+EQLFSA TTLDG+AVKIW+ SNN+VI +LST MD+R+M VSSDL Y+G R
Sbjct: 841 NIVQSLQIYEEQLFSASTTLDGAAVKIWSMSNNSVIETLSTAMDIRTMAVSSDLTYLGGR 900

Query: 901 -GVVEMWSREKYNKIDTLQTGRNCKVACMALSEREDVLVIGTSDGRIQAWGL 929
            GVVE+W R+K NKIDTLQTGR CKVACM L+EREDVL IGTSDGRIQ WGL
Sbjct: 901 GGVVEIWGRDKLNKIDTLQTGRICKVACMTLNEREDVLFIGTSDGRIQGWGL 948

BLAST of Sed0019774 vs. NCBI nr
Match: XP_022933811.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1497.3 bits (3875), Expect = 0.0e+00
Identity = 780/951 (82.02%), Postives = 848/951 (89.17%), Query Frame = 0

Query: 1   MEDSGN----------NTTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTC 60
           ME+SGN          NT PKDFVCPITCNIFYDPVTIETGQTYER+AIQEWLDRGNSTC
Sbjct: 1   MEESGNNGLESGEMHHNTHPKDFVCPITCNIFYDPVTIETGQTYERSAIQEWLDRGNSTC 60

Query: 61  PITGQKLQNTQLPKTNYVLKRLIASWLEENPTFVSEKPFG-------LTSPVNV-----I 120
           PITGQKLQNTQLPKTNYVLKRLIASWL+ENP FV++K          L SPV+V     I
Sbjct: 61  PITGQKLQNTQLPKTNYVLKRLIASWLKENPNFVTDKALDEADSAAVLISPVSVISQASI 120

Query: 121 NRTMEVRQAITNLYLSEVLDDAEAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILV 180
           NR+MEVR AI+N+  SEVL++AE+AVLCVERFWLEENV+++IQ+MLLKPPVINGLVEIL+
Sbjct: 121 NRSMEVRHAISNIVSSEVLEEAESAVLCVERFWLEENVDVEIQHMLLKPPVINGLVEILI 180

Query: 181 NSVNLHVLRASIFLLSELGFKDAAVIQMLTRVESDVDCILTLFKSGLMEAVVLIYQFGLS 240
           NSV+L VLRASIFLLSELGFKDAAVIQ LTRVESDVDCI+TLFKSGLMEAVVL+Y+FGLS
Sbjct: 181 NSVDLQVLRASIFLLSELGFKDAAVIQTLTRVESDVDCIVTLFKSGLMEAVVLMYRFGLS 240

Query: 241 VQHLQEMDLAGFLLNVVKKKGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAE 300
           +Q LQEMDLAG LLNVVKKK +DVN KM+L+ KSAAVILLRKILGRSKEGSLIAVAVLAE
Sbjct: 241 IQRLQEMDLAGSLLNVVKKK-EDVN-KMQLNPKSAAVILLRKILGRSKEGSLIAVAVLAE 300

Query: 301 NAIDSILGSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADEAHAHLASILGSFMEVSNE 360
           NAI+SIL SLKAKQVEERIAAVGILLRCIQEDGKCRN+IAD+  A LA ILGSFMEVSN+
Sbjct: 301 NAIESILVSLKAKQVEERIAAVGILLRCIQEDGKCRNMIADK--ADLAPILGSFMEVSND 360

Query: 361 EQFEIVMFLSDLVKLNRRTFNQQILQSIRDGGGCSTMHSLLIYLQTAHKDQSPVVAGLLL 420
           EQFEI+MFLS+LVKLNRRTFN+QILQ+I+DGG CSTMHSLL+YLQTA +DQ PVVAG LL
Sbjct: 361 EQFEIIMFLSELVKLNRRTFNEQILQNIKDGGECSTMHSLLVYLQTAPRDQCPVVAGFLL 420

Query: 421 QLDILVEPRKMSIYREEAMDVLFSCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYV 480
           QLDILVEPRK SIYREEAMDVL SCLG+SDFPTAQISAAETIMSLQGRFSTSGRPL RYV
Sbjct: 421 QLDILVEPRKSSIYREEAMDVLLSCLGNSDFPTAQISAAETIMSLQGRFSTSGRPLTRYV 480

Query: 481 LLERVGFMIGRMKLKPRGNNSNAPGQVKLTTEEERANDEWERKMAFVLMSHDFGLVFEPL 540
           LLERVGF  G MK K R NNS+ PG V+L+  EERA DEWERKMAFVL SHDFGL+FEPL
Sbjct: 481 LLERVGFAKGCMKPKRRDNNSSGPGDVELSIAEERATDEWERKMAFVLASHDFGLLFEPL 540

Query: 541 AKGLKSKYAALFSACFVLATWLCHMLRVLPDTGILEAARVCLLDHFISIFTTTTDIEEKA 600
           AKGLKSKYAALFSACFV ATWL HML+VLPDTGIL+ ARVCLLDHF+SIF TTTDIEEKA
Sbjct: 541 AKGLKSKYAALFSACFVSATWLSHMLKVLPDTGILDTARVCLLDHFVSIFITTTDIEEKA 600

Query: 601 LGLLAMNSFVHEPEGMQCLRSNMKSINSGLKELRRSTPLAFEMLKVLCEGEESSTELWNH 660
           L LL MNSFVH+PEG+Q L SNMK I  GLKELRRST LAFEMLKVLC+GEESS ELW+H
Sbjct: 601 LALLGMNSFVHQPEGLQYLSSNMKDIMRGLKELRRSTALAFEMLKVLCKGEESSAELWSH 660

Query: 661 QELFQVDCSKNGEVLSIVYFKDKTISGHSDGTIKVWSVRGTNIHLLQETQEHSKGVTSLA 720
           QELF VDCS+NGEVLSI YFKDK ISGHSDGTIKVWSV GTN+H LQETQEHSKGVTSLA
Sbjct: 661 QELFLVDCSRNGEVLSIAYFKDKIISGHSDGTIKVWSVGGTNLHPLQETQEHSKGVTSLA 720

Query: 721 ITESDDKLYSGSLDKTIKVWSLDSDKIQCIQVHDVKDQIHNLVVSKTVACFVPHGAGIRV 780
           I ES++KLYSGSLDKTIKVWSL SD IQCIQVHDVKDQ+HNLVVSKT+ACF+PHGAGIRV
Sbjct: 721 IIESEEKLYSGSLDKTIKVWSLGSDVIQCIQVHDVKDQVHNLVVSKTIACFIPHGAGIRV 780

Query: 781 YSWGGESKLLNSSKHVKCLDLVHGKLYCGCHDGSIQEVDLATETLSNIHSGSRKLLGKAP 840
           YSWGGESKLLNSSKHVK L+LV GKLYCGCHD SIQEVDLAT T+S IH GSRKLLGK  
Sbjct: 781 YSWGGESKLLNSSKHVKRLNLVRGKLYCGCHDSSIQEVDLATGTVSYIHIGSRKLLGKPN 840

Query: 841 SVQALQIYDEQLFSACTTLDGSAVKIWNTSNNNVIASLSTPMDVRSMIVSSDLIYVGTR- 900
            VQ+LQIYDEQLFSA TTLDG+AVKIW+ SNN+VIA+LST MD+R+M VSSDL Y+G R 
Sbjct: 841 IVQSLQIYDEQLFSASTTLDGAAVKIWSMSNNSVIATLSTAMDIRTMTVSSDLTYLGGRG 900

Query: 901 GVVEMWSREKYNKIDTLQTGRNCKVACMALSEREDVLVIGTSDGRIQAWGL 929
           GVVE+W R+K NKIDTLQTGR CKVACM L+EREDVLVIGTSDGRIQAWGL
Sbjct: 901 GVVEIWGRDKLNKIDTLQTGRICKVACMTLNEREDVLVIGTSDGRIQAWGL 947

BLAST of Sed0019774 vs. NCBI nr
Match: XP_023007487.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1495.7 bits (3871), Expect = 0.0e+00
Identity = 780/947 (82.37%), Postives = 850/947 (89.76%), Query Frame = 0

Query: 1   MEDSGN---------NTTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTCP 60
           ME+SGN         NT PKDFVCPITCNIFYDPVTIETGQTYER+AIQEWLDRGNSTCP
Sbjct: 1   MEESGNNGLESGEMHNTHPKDFVCPITCNIFYDPVTIETGQTYERSAIQEWLDRGNSTCP 60

Query: 61  ITGQKLQNTQLPKTNYVLKRLIASWLEENPTFVSEKPFG---LTSPVNV-----INRTME 120
           ITGQKLQNTQLPKTNYVLKRLIASWLEENP FV++K      LTSPV+V     INR+ME
Sbjct: 61  ITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVTDKADSAAVLTSPVSVISQASINRSME 120

Query: 121 VRQAITNLYLSEVLDDAEAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILVNSVNL 180
           VR AI+N+  SEVL++AE+AVLCVERFWLEENV+++IQ+MLLKPPVINGLVEIL+NSV+L
Sbjct: 121 VRHAISNIVSSEVLEEAESAVLCVERFWLEENVDVEIQHMLLKPPVINGLVEILINSVDL 180

Query: 181 HVLRASIFLLSELGFKDAAVIQMLTRVESDVDCILTLFKSGLMEAVVLIYQFGLSVQHLQ 240
             LRA+IFLLSELGFKDAAVIQMLTRVESDVDCI+TLFKSGLMEAVVLIY+FGLS+Q LQ
Sbjct: 181 QALRATIFLLSELGFKDAAVIQMLTRVESDVDCIVTLFKSGLMEAVVLIYRFGLSIQCLQ 240

Query: 241 EMDLAGFLLNVVKKKGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAENAIDS 300
           EMDLAG LLNVVKKK +DVN KM+LS KSAAVILLRKILGRSKEGS+IAVAVLAENAI+S
Sbjct: 241 EMDLAGSLLNVVKKK-EDVN-KMQLSPKSAAVILLRKILGRSKEGSMIAVAVLAENAIES 300

Query: 301 ILGSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADEAHAHLASILGSFMEVSNEEQFEI 360
           IL SLKAKQVEERIAAVGILLRCIQEDGKCRN+IAD+  A LA ILGSFMEVSN EQFEI
Sbjct: 301 ILVSLKAKQVEERIAAVGILLRCIQEDGKCRNMIADK--ADLAPILGSFMEVSNGEQFEI 360

Query: 361 VMFLSDLVKLNRRTFNQQILQSIRDGGGCSTMHSLLIYLQTAHKDQSPVVAGLLLQLDIL 420
           +MFLS+LVKLNRRTFN+QILQ+I+DGG CSTMHSLL+YLQTA KDQ PVVAG LLQLDIL
Sbjct: 361 IMFLSELVKLNRRTFNEQILQNIKDGGECSTMHSLLVYLQTAPKDQCPVVAGFLLQLDIL 420

Query: 421 VEPRKMSIYREEAMDVLFSCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV 480
           VEPRK SIYREEAMDVL SCLG+SDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV
Sbjct: 421 VEPRKSSIYREEAMDVLLSCLGNSDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV 480

Query: 481 GFMIGRMKLKPRGNNSNAPGQVKLTTE-EERANDEWERKMAFVLMSHDFGLVFEPLAKGL 540
           GF  G MK K R NNS+ PG V+L+ + EER+NDEWERKMAFVL SH+FGL+FEPLAKGL
Sbjct: 481 GFAKGCMKPKRRDNNSSGPGDVELSIKAEERSNDEWERKMAFVLASHNFGLLFEPLAKGL 540

Query: 541 KSKYAALFSACFVLATWLCHMLRVLPDTGILEAARVCLLDHFISIFTTTTDIEEKALGLL 600
           KSKYAALFSACFV ATWL HML+VLPDTGIL+AARVCLLDHF+SIF TTTDIEEKAL LL
Sbjct: 541 KSKYAALFSACFVSATWLSHMLKVLPDTGILDAARVCLLDHFVSIFVTTTDIEEKALALL 600

Query: 601 AMNSFVHEPEGMQCLRSNMKSINSGLKELRRSTPLAFEMLKVLCEGEESSTELWNHQELF 660
            MNSFVH+PEG+Q L SN K I  GLKELRRST LAFEMLKVLCEGEESS ELW+HQELF
Sbjct: 601 GMNSFVHQPEGLQYLSSNTKDIMRGLKELRRSTALAFEMLKVLCEGEESSAELWSHQELF 660

Query: 661 QVDCSKNGEVLSIVYFKDKTISGHSDGTIKVWSVRGTNIHLLQETQEHSKGVTSLAITES 720
            VDCSKNGEVLSI Y K++ ISGHSDGTIKVWSV GTN+HLLQETQEHSKGVTSLAI ES
Sbjct: 661 LVDCSKNGEVLSIAYLKEQIISGHSDGTIKVWSVGGTNLHLLQETQEHSKGVTSLAIIES 720

Query: 721 DDKLYSGSLDKTIKVWSLDSDKIQCIQVHDVKDQIHNLVVSKTVACFVPHGAGIRVYSWG 780
           ++KLYSGSLDKTIKVWSL SD IQCI+VHDVKDQ+HNLVVSKT+ACF+PHGAGIR YSWG
Sbjct: 721 EEKLYSGSLDKTIKVWSLGSDVIQCIKVHDVKDQVHNLVVSKTIACFIPHGAGIRGYSWG 780

Query: 781 GESKLLNSSKHVKCLDLVHGKLYCGCHDGSIQEVDLATETLSNIHSGSRKLLGKAPSVQA 840
           GESKLLNS+KHVKCL+LV GKLYCGCHD SIQEVDLAT T+S IH GSRKLLGK   VQ+
Sbjct: 781 GESKLLNSNKHVKCLNLVRGKLYCGCHDSSIQEVDLATGTVSYIHIGSRKLLGKPNIVQS 840

Query: 841 LQIYDEQLFSACTTLDGSAVKIWNTSNNNVIASLSTPMDVRSMIVSSDLIYVGTR-GVVE 900
           LQIY+EQLFSA TTLDG+AVKIW+ SNN+VIASLST MD+R+M VSSDL Y+G R GVVE
Sbjct: 841 LQIYEEQLFSASTTLDGAAVKIWSMSNNSVIASLSTAMDIRTMAVSSDLTYLGGRGGVVE 900

Query: 901 MWSREKYNKIDTLQTGRNCKVACMALSEREDVLVIGTSDGRIQAWGL 929
           +W R+K NKIDTLQTGR CKVACM L+EREDVLVIGTSDGRIQ WGL
Sbjct: 901 IWGRDKLNKIDTLQTGRICKVACMTLNEREDVLVIGTSDGRIQGWGL 943

BLAST of Sed0019774 vs. NCBI nr
Match: XP_023531662.1 (putative E3 ubiquitin-protein ligase LIN-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1495.3 bits (3870), Expect = 0.0e+00
Identity = 779/951 (81.91%), Postives = 848/951 (89.17%), Query Frame = 0

Query: 1   MEDSGN---------NTTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTCP 60
           ME+SGN         NT PKDFVCPITCNIFYDPVTIETGQTYER+AIQEWLDRGNSTCP
Sbjct: 1   MEESGNNGLESGEMHNTHPKDFVCPITCNIFYDPVTIETGQTYERSAIQEWLDRGNSTCP 60

Query: 61  ITGQKLQNTQLPKTNYVLKRLIASWLEENPTFVSEKPFG-------LTSPVNV-----IN 120
           ITGQKLQNTQLPKTNYVLKRLIASWLEENP FV++K          LTSPV+V     IN
Sbjct: 61  ITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVTDKALDEADSAAVLTSPVSVISQASIN 120

Query: 121 RTMEVRQAITNLYLSEVLDDAEAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILVN 180
            +MEVR AI+N+  SEVL++AE+AVLCVERFWLEENV+++IQ MLLKPPVINGLVEIL+N
Sbjct: 121 ISMEVRHAISNIVSSEVLEEAESAVLCVERFWLEENVDVEIQNMLLKPPVINGLVEILIN 180

Query: 181 SVNLHVLRASIFLLSELGFKDAAVIQMLTRVESDVDCILTLFKSGLMEAVVLIYQFGLSV 240
           SV+L VLRA+IFLLSELGFKDAAVIQ LTRVESDVDCI+TLFKSGLMEAVVLIY+FGLS+
Sbjct: 181 SVDLQVLRATIFLLSELGFKDAAVIQTLTRVESDVDCIVTLFKSGLMEAVVLIYRFGLSI 240

Query: 241 QHLQEMDLAGFLLNVVKKKGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAEN 300
           Q LQEMDLAG LLNVVKKK D   +KM+LS KSAAVILLRKILGRSKEGSLIAVAVLAEN
Sbjct: 241 QRLQEMDLAGSLLNVVKKKED--GNKMQLSPKSAAVILLRKILGRSKEGSLIAVAVLAEN 300

Query: 301 AIDSILGSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADEAHAHLASILGSFMEVSNEE 360
           AI+SIL SLKAKQVEERIAAVGILLRCIQEDGKCRN++AD+  A LA ILGSFMEVSN+E
Sbjct: 301 AIESILVSLKAKQVEERIAAVGILLRCIQEDGKCRNMMADK--ADLAPILGSFMEVSNDE 360

Query: 361 QFEIVMFLSDLVKLNRRTFNQQILQSIRDGGGCSTMHSLLIYLQTAHKDQSPVVAGLLLQ 420
           QFEI+MFLS+LVKLNRRTFN+QILQ+I+DGG CSTMHSLL+YLQTA +DQ PVVAG LLQ
Sbjct: 361 QFEIIMFLSELVKLNRRTFNEQILQNIKDGGECSTMHSLLVYLQTAPRDQCPVVAGFLLQ 420

Query: 421 LDILVEPRKMSIYREEAMDVLFSCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYVL 480
           LDILVEPRK SIYREEAMDVL SCLG+SDFPTAQISAAETIMSLQGRFSTSGRPLARYVL
Sbjct: 421 LDILVEPRKSSIYREEAMDVLLSCLGNSDFPTAQISAAETIMSLQGRFSTSGRPLARYVL 480

Query: 481 LERVGFMIGRMKLKPRGNNSNAPGQVKLTTE-EERANDEWERKMAFVLMSHDFGLVFEPL 540
           LERVGF  G MK K R NNS+ PG V+L+ + EERANDEWERKMAFVL+SHDFGL+FEPL
Sbjct: 481 LERVGFAKGCMKPKRRDNNSSGPGDVELSIKAEERANDEWERKMAFVLVSHDFGLLFEPL 540

Query: 541 AKGLKSKYAALFSACFVLATWLCHMLRVLPDTGILEAARVCLLDHFISIFTTTTDIEEKA 600
           AKGLKSKYAALFSACFV ATWL HML+VLPD GIL+AARVCLLDHF+SIF TTTDIEEKA
Sbjct: 541 AKGLKSKYAALFSACFVSATWLSHMLKVLPDMGILDAARVCLLDHFVSIFITTTDIEEKA 600

Query: 601 LGLLAMNSFVHEPEGMQCLRSNMKSINSGLKELRRSTPLAFEMLKVLCEGEESSTELWNH 660
           L LL MNSFVH+PEG+Q L SNMK I  GLKELRRST LAFEMLKVLCEGEESS ELW+H
Sbjct: 601 LALLGMNSFVHQPEGLQYLSSNMKDIMRGLKELRRSTALAFEMLKVLCEGEESSAELWSH 660

Query: 661 QELFQVDCSKNGEVLSIVYFKDKTISGHSDGTIKVWSVRGTNIHLLQETQEHSKGVTSLA 720
           QELF VDCSKNGEVLSI YFK+K ISGHSDGTIKVWSV GTN+HLLQETQEHSKGVTSLA
Sbjct: 661 QELFLVDCSKNGEVLSIAYFKEKIISGHSDGTIKVWSVGGTNLHLLQETQEHSKGVTSLA 720

Query: 721 ITESDDKLYSGSLDKTIKVWSLDSDKIQCIQVHDVKDQIHNLVVSKTVACFVPHGAGIRV 780
           I ES++KLYSGSLDKTIKVWSL SD IQCIQVHDVKDQ+HNLVVSKT+ACF+PHGAGIRV
Sbjct: 721 IIESEEKLYSGSLDKTIKVWSLGSDVIQCIQVHDVKDQVHNLVVSKTIACFIPHGAGIRV 780

Query: 781 YSWGGESKLLNSSKHVKCLDLVHGKLYCGCHDGSIQEVDLATETLSNIHSGSRKLLGKAP 840
           YSWG ESKLLNS+KHVKCL+LV G LYCGCHD SIQEVDLAT T+S IH GSRKLLGK  
Sbjct: 781 YSWGEESKLLNSNKHVKCLNLVRGTLYCGCHDSSIQEVDLATGTVSYIHIGSRKLLGKPN 840

Query: 841 SVQALQIYDEQLFSACTTLDGSAVKIWNTSNNNVIASLSTPMDVRSMIVSSDLIYVGTR- 900
            VQ+LQ+YDEQLFSA TTLDG+AVKIW+ SNN+VIA+LST MD+R+M VSSDL Y+G R 
Sbjct: 841 IVQSLQMYDEQLFSASTTLDGAAVKIWSMSNNSVIATLSTAMDIRTMAVSSDLTYLGGRG 900

Query: 901 GVVEMWSREKYNKIDTLQTGRNCKVACMALSEREDVLVIGTSDGRIQAWGL 929
           GVVE+W R+K NKID LQTGR CKVACM L+EREDVLVIGTSDGRIQAWGL
Sbjct: 901 GVVEIWGRDKLNKIDALQTGRICKVACMTLNEREDVLVIGTSDGRIQAWGL 947

BLAST of Sed0019774 vs. ExPASy Swiss-Prot
Match: C6L7U1 (Putative E3 ubiquitin-protein ligase LIN-1 OS=Lotus japonicus OX=34305 GN=CERBERUS PE=2 SV=2)

HSP 1 Score: 692.6 bits (1786), Expect = 6.2e-198
Identity = 399/982 (40.63%), Postives = 593/982 (60.39%), Query Frame = 0

Query: 10   PKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVL 69
            PKDFVCPIT  IF DPVT+ETGQTYER AIQEWL  GN+TCPIT Q L  + LPKTNYVL
Sbjct: 512  PKDFVCPITGQIFCDPVTLETGQTYERKAIQEWLRTGNTTCPITRQPLSASILPKTNYVL 571

Query: 70   KRLIASWLEENPTFVSE-----KPFG---------------------------------- 129
            KRLI SW E+NP    E      P G                                  
Sbjct: 572  KRLITSWKEQNPELAQEFSNVNTPRGSSCSPSAKDIPMLSTRQRTTDSPNHKNKDYARQR 631

Query: 130  ---------LTSPVNVINRTM------EVRQAITNLYLSEVLDDAEAAVLCVERFWLEEN 189
                      TSP +V+++         ++  I++L  SE L + E AVL + R   +  
Sbjct: 632  SNRFMPAAITTSPTSVLSQAAVETIVNSLKPYISSLCTSENLPECEEAVLKIARLLKDSK 691

Query: 190  VEMKIQYMLLKPPVINGLVEILVNSVNLHVLRASIFLLSELGFKDAAVIQMLTRVESDVD 249
               +I   L KP +INGLVEIL  S N  VLR SI++LSEL F D +V + L  V+SD D
Sbjct: 692  TNPQIHSYLSKPTIINGLVEILSASRNREVLRTSIYILSELIFTDDSVAETLNSVDSDFD 751

Query: 250  CILTLFKSGLMEAVVLIYQFGLSVQHLQEMDLAGFLLNVVKKKGDDVND-KMRLSQKSAA 309
            C+ TL K+GL EA +LIYQ       L   +L   L++V++ K ++++D ++ +  K AA
Sbjct: 752  CLATLLKNGLAEAALLIYQLRPVFAQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAA 811

Query: 310  VILLRKIL--GRSKEGSLIAVAVLAENAIDSILGSLKAKQVEERIAAVGILLRCIQEDGK 369
            + +L + L  G     SL A +V++ N I +++  L  +++E R + V +LL C+Q +  
Sbjct: 812  IAILEQTLMGGDEYSRSLNASSVISANGIPTLVKYL--ERMEGRRSVVSVLLCCMQAEKS 871

Query: 370  CRNIIADEAHAHLASILGSFMEVSNEEQFEIVMFLSDLVKLNRRTFNQQILQSIRDGGGC 429
            C+N+IA+     L+ +L  F   ++  +   V FLS+LV+LNRRT   QIL +I+D G  
Sbjct: 872  CKNLIAN--RIELSPVLELFHSGNDSVRGTCVEFLSELVQLNRRTSCNQILHTIKDEGAF 931

Query: 430  STMHSLLIYLQTAHKDQSPVVAGLLLQLDILVEPRKMSIYREEAMDVLFSCLGDSDFPTA 489
            STMH+ L+YLQ A  +    VA LLLQLD+L EPRKMSIYREEA++ L   L   DF   
Sbjct: 932  STMHTFLVYLQMAPMEHQLAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSNT 991

Query: 490  QISAAETIMSLQGRFSTSGRPLARYVLLERVGF---MIGRMKLKPRGNNSNAPGQVKLTT 549
            Q+ A + ++ L G  S+SG+      LL+  GF       MK++  G + N    ++   
Sbjct: 992  QMKALDALLFLIGHISSSGKSYTEAWLLKIAGFDQPYNALMKVEQLGQHDN--DLIETME 1051

Query: 550  EEERANDEWERKMAFVLMSHDFGLVFEPLAKGLKSKYAALFSACFVLATWLCHMLRVLPD 609
            +E+ A + W++++A VL +H+ G +F+ L + LKS    +  +C VLATWL HML  LPD
Sbjct: 1052 DEKNALNSWQKRIASVLCNHENGSIFKALEECLKSNSLKMAKSCLVLATWLTHMLYTLPD 1111

Query: 610  TGILEAARVCLLDHFISIFTTTTDIEEKALGLLAMNSFVHEPEGMQCLRSNMKSINSGLK 669
            TG+ + AR  LL+  I++  ++ ++EEK L  LA+ +F+ +P   + LR   KSI   L+
Sbjct: 1112 TGVRDVARKSLLEEVINVLQSSKNLEEKILATLALKTFISDPSTHEALRVYAKSIYRTLR 1171

Query: 670  ELRRSTPLAFEMLKVLCEGEE-SSTELWNHQELFQVDCSKNGEVLSIVYFKDKTISGHSD 729
             L++ + +A +++KV+   +    TELW+ +E+ ++D S NGEVLS+VY   + +SGH+D
Sbjct: 1172 RLKKYSVVAVDIMKVILNLKSVDVTELWSCKEVVELDLSSNGEVLSMVYLNGQVLSGHTD 1231

Query: 730  GTIKVWSVRGTNIHLLQETQEHSKGVTSLAITESDDKLYSGSLDKTIKVWSLDSDKIQCI 789
            GTIKVW  R     ++QET EH+K VTSL    S D+LYSGSLDKTI+VW++ SD I+CI
Sbjct: 1232 GTIKVWDARKRIPRVIQETHEHTKAVTSLC--SSGDRLYSGSLDKTIRVWTIKSDGIKCI 1291

Query: 790  QVHDVKDQIHNLVVSKTVACFVPHGAGIRVYSWGGESKLLNSSKHVKCLDLVHGKLYCGC 849
             V+D+K+ +H L  +  +AC+V  G G++V++W    KL+N SK+VK L +   KLYCGC
Sbjct: 1292 DVYDIKEAVHELAANDKLACYVSQGTGVKVFNWSEAPKLINFSKYVKSLAVAGDKLYCGC 1351

Query: 850  HDGSIQEVDLATETLSNIHSGSRKLLGKAPSVQALQIYDEQLFSACTTLDGSAVKIWNTS 909
               SIQEVDL+T T ++  +G+RKLLGK  ++ +LQI+D+ LF+  +++D +A KI++ S
Sbjct: 1352 SGYSIQEVDLSTYTSNSFFTGTRKLLGK-QTIHSLQIHDDYLFACGSSVDATAGKIFSLS 1411

Query: 910  NNNVIASLSTPMDVRSMIVSSDLIYVGTR-GVVEMWSREKYNKIDTLQ-TGRNCKVACMA 929
               V+ SLST +D+  + ++SD I+ GT+ G +E+W ++K+ ++ +++  G + K+  + 
Sbjct: 1412 QKMVVGSLSTGLDIHRIAINSDFIFAGTKFGTIEVWLKDKFTRVASIKMAGGHTKITSLV 1471

BLAST of Sed0019774 vs. ExPASy Swiss-Prot
Match: D1FP53 (Putative E3 ubiquitin-protein ligase LIN OS=Medicago truncatula OX=3880 GN=LIN PE=2 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 6.4e-195
Identity = 405/983 (41.20%), Postives = 589/983 (59.92%), Query Frame = 0

Query: 10   PKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVL 69
            PKDFVCPIT  IF DPVT+ETGQTYER AIQEWL  GN+TCPIT Q L    LPKTNYVL
Sbjct: 514  PKDFVCPITGQIFSDPVTLETGQTYERKAIQEWLGTGNTTCPITRQALSANILPKTNYVL 573

Query: 70   KRLIASWLEENPTFVSE-----KPFGLT-SP-------VNVINRTME----------VRQ 129
            KRLI SW E+NP    E      P G + SP       V+ I RT +          +RQ
Sbjct: 574  KRLIVSWKEQNPELAQEFSNSNTPRGSSCSPSAKDITMVSSIQRTTDSPSQKYKDDYIRQ 633

Query: 130  A--------------------------------ITNLYLSEVLDDAEAAVLCVERFWLEE 189
                                             IT+L  SE L D E AVL + R W + 
Sbjct: 634  RNNRFTRVSVGASPTSVLSQAAVETIINSLTPYITSLCTSENLQDCEQAVLEIARLWKDS 693

Query: 190  NVEMKIQYMLLKPPVINGLVEILVNSVNLHVLRASIFLLSELGFKDAAVIQMLTRVESDV 249
              + +I   L KP V++GLVEIL  S+N  VLR SI++LSEL F D  V + L  V+SD 
Sbjct: 694  KTDPQIHSYLSKPTVVSGLVEILSASLNREVLRRSIYILSELIFSDERVGETLNSVDSDF 753

Query: 250  DCILTLFKSGLMEAVVLIYQFGLSVQHLQEMDLAGFLLNVVKKKGDDVND-KMRLSQKSA 309
            DC+  L K+GL EA +LIYQ       L E +L   L+ V++ K +D++D ++ +  K+A
Sbjct: 754  DCLAMLLKNGLAEAALLIYQLRPVFAQLSEHELIPSLIQVIQNKSEDIDDFQLAIDPKAA 813

Query: 310  AVILLRKIL--GRSKEGSLIAVAVLAENAIDSILGSLKAKQVEERIAAVGILLRCIQEDG 369
            A+ +L +IL  G     S+ A +V++ N I +I+  L   + E R   + ILL C+Q + 
Sbjct: 814  AIAILEQILIGGDEYNRSVNASSVISANGIPAIVKYL--DKTEGRRPVISILLCCMQAEK 873

Query: 370  KCRNIIADEAHAHLASILGSFMEVSNEEQFEIVMFLSDLVKLNRRTFNQQILQSIRDGGG 429
             C++ IA+     L+ +L  F   ++  +   V FLS+LV+LNRRT + Q LQ I+D G 
Sbjct: 874  SCKSSIAN--RIELSPVLELFHAGNDSVRGICVEFLSELVRLNRRTSSNQTLQIIKDEGA 933

Query: 430  CSTMHSLLIYLQTAHKDQSPVVAGLLLQLDILVEPRKMSIYREEAMDVLFSCLGDSDFPT 489
             STMH+ L+YLQ A  +    VA LLLQLD+L EPRKMSIYREEA++ L   L   DF  
Sbjct: 934  FSTMHTFLVYLQMAPMEHQIAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSN 993

Query: 490  AQISAAETIMSLQGRFSTSGRPLARYVLLERVGF---MIGRMKLKPRGNNSNAPGQVKLT 549
             Q+ A + ++ L G  ++SG+      LL+  GF       MK +  G++ N    ++  
Sbjct: 994  NQMKALDALLFLIGHVTSSGKSYTEAGLLKIAGFDQPYNVLMKAEQLGHSDN--DFMETM 1053

Query: 550  TEEERANDEWERKMAFVLMSHDFGLVFEPLAKGLKSKYAALFSACFVLATWLCHMLRVLP 609
             +E+ A   W++++A VL +H+ G +F+ L + LKS    +  +C VLATWL HML  LP
Sbjct: 1054 EDEKNAMKSWQKRVASVLCNHENGSIFQALEECLKSNSLKMAKSCLVLATWLTHMLFTLP 1113

Query: 610  DTGILEAARVCLLDHFISIFTTTTDIEEKALGLLAMNSFVHEPEGMQCLRSNMKSINSGL 669
            DTG+ + AR  LL+  +++  ++ ++EEK L  LA+ SF+ +P   + LR   KSI   L
Sbjct: 1114 DTGVRDVARKSLLEALMNVLQSSKNLEEKILASLALKSFISDPTVHEVLRVYAKSIYRIL 1173

Query: 670  KELRRSTPLAFEMLKVLCE-GEESSTELWNHQELFQVDCSKNGEVLSIVYFKDKTISGHS 729
            ++L++ + +A ++LK L        TELW+ +E+ ++D S NGEVLS+ Y   + +SGH+
Sbjct: 1174 RKLKKYSTVAADILKALLNLNSVDVTELWSCKEVVELDLSSNGEVLSLHYLNGQVLSGHA 1233

Query: 730  DGTIKVWSVRGTNIHLLQETQEHSKGVTSLAITESDDKLYSGSLDKTIKVWSLDSDKIQC 789
            DGTIKVW  R     ++QET+EH K VTSL    S DKLYS SLDKTI+VW++  D I+C
Sbjct: 1234 DGTIKVWDARKRIPRVIQETREHKKAVTSLC--SSVDKLYSSSLDKTIRVWTIKPDGIKC 1293

Query: 790  IQVHDVKDQIHNLVVSKTVACFVPHGAGIRVYSWGGESKLLNSSKHVKCLDLVHGKLYCG 849
            I V+DVK+ ++ L  +  +AC+V  G G++V++W    K +N +K+VKCL +   KLYCG
Sbjct: 1294 IDVYDVKEAVYELAANAKLACYVTQGTGVKVFNWLDAPKFINFNKYVKCLAVSGDKLYCG 1353

Query: 850  CHDGSIQEVDLATETLSNIHSGSRKLLGKAPSVQALQIYDEQLFSACTTLDGSAVKIWNT 909
            C   SIQEVDL+  T ++  +G+RKLLGK  ++ +LQI+D+ LF+  +++D +A KI++ 
Sbjct: 1354 CSGYSIQEVDLSKYTSTSFFTGTRKLLGK-QTIHSLQIHDDLLFACGSSIDATAGKIFSL 1413

Query: 910  SNNNVIASLSTPMDVRSMIVSSDLIYVGTR-GVVEMWSREKYNKIDTLQ-TGRNCKVACM 929
            S+  V+ SLST +DV  + ++SD I+ GT+ G +E+W ++K+ ++ +++  G N K+  +
Sbjct: 1414 SSKMVVGSLSTGLDVHRVAINSDFIFAGTKFGTIEVWLKDKFTRVASIKMAGGNTKITSL 1473

BLAST of Sed0019774 vs. ExPASy Swiss-Prot
Match: D1FP57 (Putative E3 ubiquitin-protein ligase LIN-2 OS=Lotus japonicus OX=34305 GN=LIN PE=1 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 1.8e-189
Identity = 391/982 (39.82%), Postives = 581/982 (59.16%), Query Frame = 0

Query: 10   PKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVL 69
            PKDFVCPIT  IF DPVT+ETGQTYER AIQEWL  GN+TCPIT Q L  + LPKTNYVL
Sbjct: 512  PKDFVCPITGQIFCDPVTLETGQTYERKAIQEWLRTGNTTCPITRQPLSASILPKTNYVL 571

Query: 70   KRLIASWLEENPTFVSE-----KPFG---------------------------------- 129
            KRLI SW E+NP    E      P G                                  
Sbjct: 572  KRLITSWKEQNPELAQEFSNVNTPRGSSCSPSAKDIPMLSTRQRTTDSPNHKNKDYARQR 631

Query: 130  ---------LTSPVNVINRTM------EVRQAITNLYLSEVLDDAEAAVLCVERFWLEEN 189
                      TSP +V+++         ++  I++L  SE L + E AVL + R   +  
Sbjct: 632  SNRFMPAAITTSPTSVLSQAAVETIVNSLKPYISSLCTSENLPECEEAVLKIARLLKDSK 691

Query: 190  VEMKIQYMLLKPPVINGLVEILVNSVNLHVLRASIFLLSELGFKDAAVIQMLTRVESDVD 249
               +I   L KP +INGLVEIL  S N  VLR SI++LSEL F D +V + L  V+SD D
Sbjct: 692  TNPQIHSYLSKPTIINGLVEILSASRNREVLRTSIYILSELIFTDDSVAETLNSVDSDFD 751

Query: 250  CILTLFKSGLMEAVVLIYQFGLSVQHLQEMDLAGFLLNVVKKKGDDVND-KMRLSQKSAA 309
            C+ TL K+GL EA +LIYQ       L   +L   L++V++ K ++++D ++ +  K AA
Sbjct: 752  CLATLLKNGLAEAALLIYQLRPVFAQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAA 811

Query: 310  VILLRKIL--GRSKEGSLIAVAVLAENAIDSILGSLKAKQVEERIAAVGILLRCIQEDGK 369
            + +L + L  G     SL A +V++ N I +++  L  +++E R + V +LL C+Q +  
Sbjct: 812  IAILEQTLMGGDEYSRSLNASSVISANGIPTLVKYL--ERMEGRRSVVSVLLCCMQAEKS 871

Query: 370  CRNIIADEAHAHLASILGSFMEVSNEEQFEIVMFLSDLVKLNRRTFNQQILQSIRDGGGC 429
            C+N+IA+     L+ +L  F   ++  +   V FLS+LV+LNRRT   Q+L +I+D G  
Sbjct: 872  CKNLIAN--RIELSPVLELFHSGNDSVRGTCVEFLSELVQLNRRTSCNQLLHTIKDEGAF 931

Query: 430  STMHSLLIYLQTAHKDQSPVVAGLLLQLDILVEPRKMSIYREEAMDVLFSCLGDSDFPTA 489
            STMH+ L+YLQ A  +    VA LLLQLD+L EPRKMSIYREEA++ L   L   DF   
Sbjct: 932  STMHTFLVYLQMAPMEHQLAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSNT 991

Query: 490  QISAAETIMSLQGRFSTSGRPLARYVLLERVGF---MIGRMKLKPRGNNSNAPGQVKLTT 549
            Q+ A + ++ L G  S+SG+      LL+  GF       MK++  G + N    ++   
Sbjct: 992  QMKALDALLFLIGHISSSGKSYTEAWLLKIAGFDQPYNALMKVEQLGQHDN--DLIETME 1051

Query: 550  EEERANDEWERKMAFVLMSHDFGLVFEPLAKGLKSKYAALFSACFVLATWLCHMLRVLPD 609
            +E+ A + W++++A VL +H+ G +F+ L + LKS    +  +C VLATWL  ML  LPD
Sbjct: 1052 DEKNALNSWQKRIASVLCNHENGSIFKALEECLKSNSLKMAKSCLVLATWLTRMLYTLPD 1111

Query: 610  TGILEAARVCLLDHFISIFTTTTDIEEKALGLLAMNSFVHEPEGMQCLRSNMKSINSGLK 669
            TG+ + AR  LL+  I +  ++  +E+  L  L++  F+ +P   + LR   KSI   L+
Sbjct: 1112 TGVRDVARKSLLEEVIKVLHSSKSLEDMILVTLSLYPFISDPTVHEVLRVYAKSIYRILR 1171

Query: 670  ELRRSTPLAFEMLKVLCE-GEESSTELWNHQELFQVDCSKNGEVLSIVYFKDKTISGHSD 729
            +L++ + +A ++LK L        TELW+ +E+ ++D S NGEVLS+ Y   + +SG  D
Sbjct: 1172 KLKKYSTVAADILKALLNLNSVDVTELWSCKEVVELDLSSNGEVLSLHYLNGQVLSGLMD 1231

Query: 730  GTIKVWSVRGTNIHLLQETQEHSKGVTSLAITESDDKLYSGSLDKTIKVWSLDSDKIQCI 789
            GT KV   R     ++QET EH+K VTSL    S D+LYS SLDKTI+VW++ SD I+CI
Sbjct: 1232 GTSKVCDARKRIPRVIQETHEHTKAVTSLC--SSGDRLYSASLDKTIRVWTIKSDGIKCI 1291

Query: 790  QVHDVKDQIHNLVVSKTVACFVPHGAGIRVYSWGGESKLLNSSKHVKCLDLVHGKLYCGC 849
             V+D+K+ +H L  +  +AC+V  G G++V++W    KL+N SK+VK L +   KLYCGC
Sbjct: 1292 DVYDIKEAVHELAANDKLACYVSQGTGVKVFNWSEAPKLINFSKYVKSLAVAGDKLYCGC 1351

Query: 850  HDGSIQEVDLATETLSNIHSGSRKLLGKAPSVQALQIYDEQLFSACTTLDGSAVKIWNTS 909
               SIQEVDL+T T ++  +G+RKLLGK  ++ +LQI+D+ LF+  +++D +A KI++ S
Sbjct: 1352 SGYSIQEVDLSTYTSNSFFTGTRKLLGK-QTIHSLQIHDDYLFACVSSVDATAGKIFSLS 1411

Query: 910  NNNVIASLSTPMDVRSMIVSSDLIYVGTR-GVVEMWSREKYNKIDTLQ-TGRNCKVACMA 929
               V+ SLST +D+  + ++SD I+ GT+ G +E+W ++K+ ++ ++Q  G + K+  + 
Sbjct: 1412 QKMVVGSLSTGLDIHRIAINSDFIFAGTKFGTIEVWLKDKFTRVASIQMAGGHTKITSLV 1471

BLAST of Sed0019774 vs. ExPASy Swiss-Prot
Match: Q9C7R6 (U-box domain-containing protein 17 OS=Arabidopsis thaliana OX=3702 GN=PUB17 PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.3e-14
Identity = 84/320 (26.25%), Postives = 144/320 (45.00%), Query Frame = 0

Query: 8   TTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTCPITGQKLQNTQLPKTNY 67
           T PKDFVCPI+ ++  DPV I TGQTY+RN+I  W++ G+ TCP TGQ L ++++   N 
Sbjct: 304 TVPKDFVCPISLDLMTDPVIISTGQTYDRNSIARWIEEGHCTCPKTGQMLMDSRI-VPNR 363

Query: 68  VLKRLIASW-------LEENPTFVSEKPFGLTSPVNVINRTMEVRQAITNLYLSEVLDDA 127
            LK LI  W        E   T    + F    P       +E  +A  ++ +  + D +
Sbjct: 364 ALKNLIVQWCTASGISYESEFTDSPNESFASALPTKA---AVEANKATVSILIKYLADGS 423

Query: 128 EAA-VLCVERFWLEENVEMKIQYMLLKPPVINGLVEILVNSVNLHVLRASIFLLSELGFK 187
           +AA  +      L      + +  + +   I  L  +L  S N      S+  +  L   
Sbjct: 424 QAAQTVAAREIRLLAKTGKENRAYIAEAGAIPHLCRLL-TSENAIAQENSVTAMLNLSIY 483

Query: 188 DAAVIQMLTRVESDVDCILTLFKSGLMEAVVLIYQFGLSVQHLQEMDLAGFLLNVVK--K 247
           +    +  +R+  + DC         +E++V +   GL+V+  +      F L+ V   K
Sbjct: 484 E----KNKSRIMEEGDC---------LESIVSVLVSGLTVEAQENAAATLFSLSAVHEYK 543

Query: 248 KGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAEN-----------AIDSILG 306
           K   + D+       A  +LL+    R K+ ++ A+  L+ +            + S++G
Sbjct: 544 KRIAIVDQC----VEALALLLQNGTPRGKKDAVTALYNLSTHPDNCSRMIEGGGVSSLVG 601

BLAST of Sed0019774 vs. ExPASy Swiss-Prot
Match: Q9C9A6 (U-box domain-containing protein 10 OS=Arabidopsis thaliana OX=3702 GN=PUB10 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 3.8e-14
Identity = 72/311 (23.15%), Postives = 145/311 (46.62%), Query Frame = 0

Query: 2   EDSGNNTTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTCPITGQKLQNTQ 61
           + S N T P+DF+CPI+  +  DP  + TGQTYER+ IQ W+D GN +CP T QKL+N  
Sbjct: 236 QKSDNLTIPEDFLCPISLELMKDPAIVSTGQTYERSFIQRWIDCGNLSCPKTQQKLENFT 295

Query: 62  LPKTNYVLKRLIASW-----LEENPTFVSEKPFGLTSPVNVINRTMEVRQAITNLYLSEV 121
           L   NYVL+ LI+ W     +E+   +++ +          ++  M   +A+     S+ 
Sbjct: 296 L-TPNYVLRSLISQWCTKHNIEQPGGYMNGRTKNSDGSFRDLSGDMSAIRALVCKLSSQS 355

Query: 122 LDDAEAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILVNSVNLHVLRASIFLLSEL 181
           ++D   AV  + R   + + + +I  ++ +   I  LV++L +  +      ++  +  L
Sbjct: 356 IEDRRTAVSEI-RSLSKRSTDNRI--LIAEAGAIPVLVKLLTSDGDTETQENAVTCILNL 415

Query: 182 GFKDAAVIQMLTRVESDVDCILTLFKSGLME----AVVLIYQFGLSVQHLQEMDLAGFLL 241
              +    + L  +   V  I+ + ++G ME    A   ++   L+ ++   +  +G ++
Sbjct: 416 SIYEHN--KELIMLAGAVTSIVLVLRAGSMEARENAAATLFSLSLADENKIIIGASGAIM 475

Query: 242 NVVKKKGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAENAIDSILGSLKAKQ 301
            +V    D +       +K AA  L    + +  +G  +   ++ +  +  +  S   + 
Sbjct: 476 ALV----DLLQYGSVRGKKDAATALFNLCIYQGNKGRAVRAGIV-KPLVKMLTDSSSERM 535

Query: 302 VEERIAAVGIL 304
            +E +  + +L
Sbjct: 536 ADEALTILSVL 535

BLAST of Sed0019774 vs. ExPASy TrEMBL
Match: A0A6J1L345 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499961 PE=4 SV=1)

HSP 1 Score: 1499.6 bits (3881), Expect = 0.0e+00
Identity = 780/946 (82.45%), Postives = 849/946 (89.75%), Query Frame = 0

Query: 1   MEDSGN---------NTTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTCP 60
           ME+SGN         NT PKDFVCPITCNIFYDPVTIETGQTYER+AIQEWLDRGNSTCP
Sbjct: 1   MEESGNNGLESGEMHNTHPKDFVCPITCNIFYDPVTIETGQTYERSAIQEWLDRGNSTCP 60

Query: 61  ITGQKLQNTQLPKTNYVLKRLIASWLEENPTFVSEKPFG---LTSPVNV-----INRTME 120
           ITGQKLQNTQLPKTNYVLKRLIASWLEENP FV++K      LTSPV+V     INR+ME
Sbjct: 61  ITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVTDKADSAAVLTSPVSVISQASINRSME 120

Query: 121 VRQAITNLYLSEVLDDAEAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILVNSVNL 180
           VR AI+N+  SEVL++AE+AVLCVERFWLEENV+++IQ+MLLKPPVINGLVEIL+NSV+L
Sbjct: 121 VRHAISNIVSSEVLEEAESAVLCVERFWLEENVDVEIQHMLLKPPVINGLVEILINSVDL 180

Query: 181 HVLRASIFLLSELGFKDAAVIQMLTRVESDVDCILTLFKSGLMEAVVLIYQFGLSVQHLQ 240
             LRA+IFLLSELGFKDAAVIQMLTRVESDVDCI+TLFKSGLMEAVVLIY+FGLS+Q LQ
Sbjct: 181 QALRATIFLLSELGFKDAAVIQMLTRVESDVDCIVTLFKSGLMEAVVLIYRFGLSIQCLQ 240

Query: 241 EMDLAGFLLNVVKKKGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAENAIDS 300
           EMDLAG LLNVVKKK +DVN KM+LS KSAAVILLRKILGRSKEGS+IAVAVLAENAI+S
Sbjct: 241 EMDLAGSLLNVVKKK-EDVN-KMQLSPKSAAVILLRKILGRSKEGSMIAVAVLAENAIES 300

Query: 301 ILGSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADEAHAHLASILGSFMEVSNEEQFEI 360
           IL SLKAKQVEERIAAVGILLRCIQEDGKCRN+IAD+  A LA ILGSFMEVSN EQFEI
Sbjct: 301 ILVSLKAKQVEERIAAVGILLRCIQEDGKCRNMIADK--ADLAPILGSFMEVSNGEQFEI 360

Query: 361 VMFLSDLVKLNRRTFNQQILQSIRDGGGCSTMHSLLIYLQTAHKDQSPVVAGLLLQLDIL 420
           +MFLS+LVKLNRRTFN+QILQ+I+DGG CSTMHSLL+YLQTA KDQ PVVAG LLQLDIL
Sbjct: 361 IMFLSELVKLNRRTFNEQILQNIKDGGECSTMHSLLVYLQTAPKDQCPVVAGFLLQLDIL 420

Query: 421 VEPRKMSIYREEAMDVLFSCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV 480
           VEPRK SIYREEAMDVL SCLG+SDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV
Sbjct: 421 VEPRKSSIYREEAMDVLLSCLGNSDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV 480

Query: 481 GFMIGRMKLKPRGNNSNAPGQVKLTTEEERANDEWERKMAFVLMSHDFGLVFEPLAKGLK 540
           GF  G MK K R NNS+ PG V+L+  EER+NDEWERKMAFVL SH+FGL+FEPLAKGLK
Sbjct: 481 GFAKGCMKPKRRDNNSSGPGDVELSIAEERSNDEWERKMAFVLASHNFGLLFEPLAKGLK 540

Query: 541 SKYAALFSACFVLATWLCHMLRVLPDTGILEAARVCLLDHFISIFTTTTDIEEKALGLLA 600
           SKYAALFSACFV ATWL HML+VLPDTGIL+AARVCLLDHF+SIF TTTDIEEKAL LL 
Sbjct: 541 SKYAALFSACFVSATWLSHMLKVLPDTGILDAARVCLLDHFVSIFVTTTDIEEKALALLG 600

Query: 601 MNSFVHEPEGMQCLRSNMKSINSGLKELRRSTPLAFEMLKVLCEGEESSTELWNHQELFQ 660
           MNSFVH+PEG+Q L SN K I  GLKELRRST LAFEMLKVLCEGEESS ELW+HQELF 
Sbjct: 601 MNSFVHQPEGLQYLSSNTKDIMRGLKELRRSTALAFEMLKVLCEGEESSAELWSHQELFL 660

Query: 661 VDCSKNGEVLSIVYFKDKTISGHSDGTIKVWSVRGTNIHLLQETQEHSKGVTSLAITESD 720
           VDCSKNGEVLSI Y K++ ISGHSDGTIKVWSV GTN+HLLQETQEHSKGVTSLAI ES+
Sbjct: 661 VDCSKNGEVLSIAYLKEQIISGHSDGTIKVWSVGGTNLHLLQETQEHSKGVTSLAIIESE 720

Query: 721 DKLYSGSLDKTIKVWSLDSDKIQCIQVHDVKDQIHNLVVSKTVACFVPHGAGIRVYSWGG 780
           +KLYSGSLDKTIKVWSL SD IQCI+VHDVKDQ+HNLVVSKT+ACF+PHGAGIR YSWGG
Sbjct: 721 EKLYSGSLDKTIKVWSLGSDVIQCIKVHDVKDQVHNLVVSKTIACFIPHGAGIRGYSWGG 780

Query: 781 ESKLLNSSKHVKCLDLVHGKLYCGCHDGSIQEVDLATETLSNIHSGSRKLLGKAPSVQAL 840
           ESKLLNS+KHVKCL+LV GKLYCGCHD SIQEVDLAT T+S IH GSRKLLGK   VQ+L
Sbjct: 781 ESKLLNSNKHVKCLNLVRGKLYCGCHDSSIQEVDLATGTVSYIHIGSRKLLGKPNIVQSL 840

Query: 841 QIYDEQLFSACTTLDGSAVKIWNTSNNNVIASLSTPMDVRSMIVSSDLIYVGTR-GVVEM 900
           QIY+EQLFSA TTLDG+AVKIW+ SNN+VIASLST MD+R+M VSSDL Y+G R GVVE+
Sbjct: 841 QIYEEQLFSASTTLDGAAVKIWSMSNNSVIASLSTAMDIRTMAVSSDLTYLGGRGGVVEI 900

Query: 901 WSREKYNKIDTLQTGRNCKVACMALSEREDVLVIGTSDGRIQAWGL 929
           W R+K NKIDTLQTGR CKVACM L+EREDVLVIGTSDGRIQ WGL
Sbjct: 901 WGRDKLNKIDTLQTGRICKVACMTLNEREDVLVIGTSDGRIQGWGL 942

BLAST of Sed0019774 vs. ExPASy TrEMBL
Match: A0A6J1F029 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441113 PE=4 SV=1)

HSP 1 Score: 1497.3 bits (3875), Expect = 0.0e+00
Identity = 780/951 (82.02%), Postives = 848/951 (89.17%), Query Frame = 0

Query: 1   MEDSGN----------NTTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTC 60
           ME+SGN          NT PKDFVCPITCNIFYDPVTIETGQTYER+AIQEWLDRGNSTC
Sbjct: 1   MEESGNNGLESGEMHHNTHPKDFVCPITCNIFYDPVTIETGQTYERSAIQEWLDRGNSTC 60

Query: 61  PITGQKLQNTQLPKTNYVLKRLIASWLEENPTFVSEKPFG-------LTSPVNV-----I 120
           PITGQKLQNTQLPKTNYVLKRLIASWL+ENP FV++K          L SPV+V     I
Sbjct: 61  PITGQKLQNTQLPKTNYVLKRLIASWLKENPNFVTDKALDEADSAAVLISPVSVISQASI 120

Query: 121 NRTMEVRQAITNLYLSEVLDDAEAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILV 180
           NR+MEVR AI+N+  SEVL++AE+AVLCVERFWLEENV+++IQ+MLLKPPVINGLVEIL+
Sbjct: 121 NRSMEVRHAISNIVSSEVLEEAESAVLCVERFWLEENVDVEIQHMLLKPPVINGLVEILI 180

Query: 181 NSVNLHVLRASIFLLSELGFKDAAVIQMLTRVESDVDCILTLFKSGLMEAVVLIYQFGLS 240
           NSV+L VLRASIFLLSELGFKDAAVIQ LTRVESDVDCI+TLFKSGLMEAVVL+Y+FGLS
Sbjct: 181 NSVDLQVLRASIFLLSELGFKDAAVIQTLTRVESDVDCIVTLFKSGLMEAVVLMYRFGLS 240

Query: 241 VQHLQEMDLAGFLLNVVKKKGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAE 300
           +Q LQEMDLAG LLNVVKKK +DVN KM+L+ KSAAVILLRKILGRSKEGSLIAVAVLAE
Sbjct: 241 IQRLQEMDLAGSLLNVVKKK-EDVN-KMQLNPKSAAVILLRKILGRSKEGSLIAVAVLAE 300

Query: 301 NAIDSILGSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADEAHAHLASILGSFMEVSNE 360
           NAI+SIL SLKAKQVEERIAAVGILLRCIQEDGKCRN+IAD+  A LA ILGSFMEVSN+
Sbjct: 301 NAIESILVSLKAKQVEERIAAVGILLRCIQEDGKCRNMIADK--ADLAPILGSFMEVSND 360

Query: 361 EQFEIVMFLSDLVKLNRRTFNQQILQSIRDGGGCSTMHSLLIYLQTAHKDQSPVVAGLLL 420
           EQFEI+MFLS+LVKLNRRTFN+QILQ+I+DGG CSTMHSLL+YLQTA +DQ PVVAG LL
Sbjct: 361 EQFEIIMFLSELVKLNRRTFNEQILQNIKDGGECSTMHSLLVYLQTAPRDQCPVVAGFLL 420

Query: 421 QLDILVEPRKMSIYREEAMDVLFSCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYV 480
           QLDILVEPRK SIYREEAMDVL SCLG+SDFPTAQISAAETIMSLQGRFSTSGRPL RYV
Sbjct: 421 QLDILVEPRKSSIYREEAMDVLLSCLGNSDFPTAQISAAETIMSLQGRFSTSGRPLTRYV 480

Query: 481 LLERVGFMIGRMKLKPRGNNSNAPGQVKLTTEEERANDEWERKMAFVLMSHDFGLVFEPL 540
           LLERVGF  G MK K R NNS+ PG V+L+  EERA DEWERKMAFVL SHDFGL+FEPL
Sbjct: 481 LLERVGFAKGCMKPKRRDNNSSGPGDVELSIAEERATDEWERKMAFVLASHDFGLLFEPL 540

Query: 541 AKGLKSKYAALFSACFVLATWLCHMLRVLPDTGILEAARVCLLDHFISIFTTTTDIEEKA 600
           AKGLKSKYAALFSACFV ATWL HML+VLPDTGIL+ ARVCLLDHF+SIF TTTDIEEKA
Sbjct: 541 AKGLKSKYAALFSACFVSATWLSHMLKVLPDTGILDTARVCLLDHFVSIFITTTDIEEKA 600

Query: 601 LGLLAMNSFVHEPEGMQCLRSNMKSINSGLKELRRSTPLAFEMLKVLCEGEESSTELWNH 660
           L LL MNSFVH+PEG+Q L SNMK I  GLKELRRST LAFEMLKVLC+GEESS ELW+H
Sbjct: 601 LALLGMNSFVHQPEGLQYLSSNMKDIMRGLKELRRSTALAFEMLKVLCKGEESSAELWSH 660

Query: 661 QELFQVDCSKNGEVLSIVYFKDKTISGHSDGTIKVWSVRGTNIHLLQETQEHSKGVTSLA 720
           QELF VDCS+NGEVLSI YFKDK ISGHSDGTIKVWSV GTN+H LQETQEHSKGVTSLA
Sbjct: 661 QELFLVDCSRNGEVLSIAYFKDKIISGHSDGTIKVWSVGGTNLHPLQETQEHSKGVTSLA 720

Query: 721 ITESDDKLYSGSLDKTIKVWSLDSDKIQCIQVHDVKDQIHNLVVSKTVACFVPHGAGIRV 780
           I ES++KLYSGSLDKTIKVWSL SD IQCIQVHDVKDQ+HNLVVSKT+ACF+PHGAGIRV
Sbjct: 721 IIESEEKLYSGSLDKTIKVWSLGSDVIQCIQVHDVKDQVHNLVVSKTIACFIPHGAGIRV 780

Query: 781 YSWGGESKLLNSSKHVKCLDLVHGKLYCGCHDGSIQEVDLATETLSNIHSGSRKLLGKAP 840
           YSWGGESKLLNSSKHVK L+LV GKLYCGCHD SIQEVDLAT T+S IH GSRKLLGK  
Sbjct: 781 YSWGGESKLLNSSKHVKRLNLVRGKLYCGCHDSSIQEVDLATGTVSYIHIGSRKLLGKPN 840

Query: 841 SVQALQIYDEQLFSACTTLDGSAVKIWNTSNNNVIASLSTPMDVRSMIVSSDLIYVGTR- 900
            VQ+LQIYDEQLFSA TTLDG+AVKIW+ SNN+VIA+LST MD+R+M VSSDL Y+G R 
Sbjct: 841 IVQSLQIYDEQLFSASTTLDGAAVKIWSMSNNSVIATLSTAMDIRTMTVSSDLTYLGGRG 900

Query: 901 GVVEMWSREKYNKIDTLQTGRNCKVACMALSEREDVLVIGTSDGRIQAWGL 929
           GVVE+W R+K NKIDTLQTGR CKVACM L+EREDVLVIGTSDGRIQAWGL
Sbjct: 901 GVVEIWGRDKLNKIDTLQTGRICKVACMTLNEREDVLVIGTSDGRIQAWGL 947

BLAST of Sed0019774 vs. ExPASy TrEMBL
Match: A0A6J1KYU9 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499961 PE=4 SV=1)

HSP 1 Score: 1495.7 bits (3871), Expect = 0.0e+00
Identity = 780/947 (82.37%), Postives = 850/947 (89.76%), Query Frame = 0

Query: 1   MEDSGN---------NTTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTCP 60
           ME+SGN         NT PKDFVCPITCNIFYDPVTIETGQTYER+AIQEWLDRGNSTCP
Sbjct: 1   MEESGNNGLESGEMHNTHPKDFVCPITCNIFYDPVTIETGQTYERSAIQEWLDRGNSTCP 60

Query: 61  ITGQKLQNTQLPKTNYVLKRLIASWLEENPTFVSEKPFG---LTSPVNV-----INRTME 120
           ITGQKLQNTQLPKTNYVLKRLIASWLEENP FV++K      LTSPV+V     INR+ME
Sbjct: 61  ITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVTDKADSAAVLTSPVSVISQASINRSME 120

Query: 121 VRQAITNLYLSEVLDDAEAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILVNSVNL 180
           VR AI+N+  SEVL++AE+AVLCVERFWLEENV+++IQ+MLLKPPVINGLVEIL+NSV+L
Sbjct: 121 VRHAISNIVSSEVLEEAESAVLCVERFWLEENVDVEIQHMLLKPPVINGLVEILINSVDL 180

Query: 181 HVLRASIFLLSELGFKDAAVIQMLTRVESDVDCILTLFKSGLMEAVVLIYQFGLSVQHLQ 240
             LRA+IFLLSELGFKDAAVIQMLTRVESDVDCI+TLFKSGLMEAVVLIY+FGLS+Q LQ
Sbjct: 181 QALRATIFLLSELGFKDAAVIQMLTRVESDVDCIVTLFKSGLMEAVVLIYRFGLSIQCLQ 240

Query: 241 EMDLAGFLLNVVKKKGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAENAIDS 300
           EMDLAG LLNVVKKK +DVN KM+LS KSAAVILLRKILGRSKEGS+IAVAVLAENAI+S
Sbjct: 241 EMDLAGSLLNVVKKK-EDVN-KMQLSPKSAAVILLRKILGRSKEGSMIAVAVLAENAIES 300

Query: 301 ILGSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADEAHAHLASILGSFMEVSNEEQFEI 360
           IL SLKAKQVEERIAAVGILLRCIQEDGKCRN+IAD+  A LA ILGSFMEVSN EQFEI
Sbjct: 301 ILVSLKAKQVEERIAAVGILLRCIQEDGKCRNMIADK--ADLAPILGSFMEVSNGEQFEI 360

Query: 361 VMFLSDLVKLNRRTFNQQILQSIRDGGGCSTMHSLLIYLQTAHKDQSPVVAGLLLQLDIL 420
           +MFLS+LVKLNRRTFN+QILQ+I+DGG CSTMHSLL+YLQTA KDQ PVVAG LLQLDIL
Sbjct: 361 IMFLSELVKLNRRTFNEQILQNIKDGGECSTMHSLLVYLQTAPKDQCPVVAGFLLQLDIL 420

Query: 421 VEPRKMSIYREEAMDVLFSCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV 480
           VEPRK SIYREEAMDVL SCLG+SDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV
Sbjct: 421 VEPRKSSIYREEAMDVLLSCLGNSDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV 480

Query: 481 GFMIGRMKLKPRGNNSNAPGQVKLTTE-EERANDEWERKMAFVLMSHDFGLVFEPLAKGL 540
           GF  G MK K R NNS+ PG V+L+ + EER+NDEWERKMAFVL SH+FGL+FEPLAKGL
Sbjct: 481 GFAKGCMKPKRRDNNSSGPGDVELSIKAEERSNDEWERKMAFVLASHNFGLLFEPLAKGL 540

Query: 541 KSKYAALFSACFVLATWLCHMLRVLPDTGILEAARVCLLDHFISIFTTTTDIEEKALGLL 600
           KSKYAALFSACFV ATWL HML+VLPDTGIL+AARVCLLDHF+SIF TTTDIEEKAL LL
Sbjct: 541 KSKYAALFSACFVSATWLSHMLKVLPDTGILDAARVCLLDHFVSIFVTTTDIEEKALALL 600

Query: 601 AMNSFVHEPEGMQCLRSNMKSINSGLKELRRSTPLAFEMLKVLCEGEESSTELWNHQELF 660
            MNSFVH+PEG+Q L SN K I  GLKELRRST LAFEMLKVLCEGEESS ELW+HQELF
Sbjct: 601 GMNSFVHQPEGLQYLSSNTKDIMRGLKELRRSTALAFEMLKVLCEGEESSAELWSHQELF 660

Query: 661 QVDCSKNGEVLSIVYFKDKTISGHSDGTIKVWSVRGTNIHLLQETQEHSKGVTSLAITES 720
            VDCSKNGEVLSI Y K++ ISGHSDGTIKVWSV GTN+HLLQETQEHSKGVTSLAI ES
Sbjct: 661 LVDCSKNGEVLSIAYLKEQIISGHSDGTIKVWSVGGTNLHLLQETQEHSKGVTSLAIIES 720

Query: 721 DDKLYSGSLDKTIKVWSLDSDKIQCIQVHDVKDQIHNLVVSKTVACFVPHGAGIRVYSWG 780
           ++KLYSGSLDKTIKVWSL SD IQCI+VHDVKDQ+HNLVVSKT+ACF+PHGAGIR YSWG
Sbjct: 721 EEKLYSGSLDKTIKVWSLGSDVIQCIKVHDVKDQVHNLVVSKTIACFIPHGAGIRGYSWG 780

Query: 781 GESKLLNSSKHVKCLDLVHGKLYCGCHDGSIQEVDLATETLSNIHSGSRKLLGKAPSVQA 840
           GESKLLNS+KHVKCL+LV GKLYCGCHD SIQEVDLAT T+S IH GSRKLLGK   VQ+
Sbjct: 781 GESKLLNSNKHVKCLNLVRGKLYCGCHDSSIQEVDLATGTVSYIHIGSRKLLGKPNIVQS 840

Query: 841 LQIYDEQLFSACTTLDGSAVKIWNTSNNNVIASLSTPMDVRSMIVSSDLIYVGTR-GVVE 900
           LQIY+EQLFSA TTLDG+AVKIW+ SNN+VIASLST MD+R+M VSSDL Y+G R GVVE
Sbjct: 841 LQIYEEQLFSASTTLDGAAVKIWSMSNNSVIASLSTAMDIRTMAVSSDLTYLGGRGGVVE 900

Query: 901 MWSREKYNKIDTLQTGRNCKVACMALSEREDVLVIGTSDGRIQAWGL 929
           +W R+K NKIDTLQTGR CKVACM L+EREDVLVIGTSDGRIQ WGL
Sbjct: 901 IWGRDKLNKIDTLQTGRICKVACMTLNEREDVLVIGTSDGRIQGWGL 943

BLAST of Sed0019774 vs. ExPASy TrEMBL
Match: A0A6J1F5V6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441113 PE=4 SV=1)

HSP 1 Score: 1493.4 bits (3865), Expect = 0.0e+00
Identity = 780/952 (81.93%), Postives = 849/952 (89.18%), Query Frame = 0

Query: 1   MEDSGN----------NTTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTC 60
           ME+SGN          NT PKDFVCPITCNIFYDPVTIETGQTYER+AIQEWLDRGNSTC
Sbjct: 1   MEESGNNGLESGEMHHNTHPKDFVCPITCNIFYDPVTIETGQTYERSAIQEWLDRGNSTC 60

Query: 61  PITGQKLQNTQLPKTNYVLKRLIASWLEENPTFVSEKPFG-------LTSPVNV-----I 120
           PITGQKLQNTQLPKTNYVLKRLIASWL+ENP FV++K          L SPV+V     I
Sbjct: 61  PITGQKLQNTQLPKTNYVLKRLIASWLKENPNFVTDKALDEADSAAVLISPVSVISQASI 120

Query: 121 NRTMEVRQAITNLYLSEVLDDAEAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILV 180
           NR+MEVR AI+N+  SEVL++AE+AVLCVERFWLEENV+++IQ+MLLKPPVINGLVEIL+
Sbjct: 121 NRSMEVRHAISNIVSSEVLEEAESAVLCVERFWLEENVDVEIQHMLLKPPVINGLVEILI 180

Query: 181 NSVNLHVLRASIFLLSELGFKDAAVIQMLTRVESDVDCILTLFKSGLMEAVVLIYQFGLS 240
           NSV+L VLRASIFLLSELGFKDAAVIQ LTRVESDVDCI+TLFKSGLMEAVVL+Y+FGLS
Sbjct: 181 NSVDLQVLRASIFLLSELGFKDAAVIQTLTRVESDVDCIVTLFKSGLMEAVVLMYRFGLS 240

Query: 241 VQHLQEMDLAGFLLNVVKKKGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAE 300
           +Q LQEMDLAG LLNVVKKK +DVN KM+L+ KSAAVILLRKILGRSKEGSLIAVAVLAE
Sbjct: 241 IQRLQEMDLAGSLLNVVKKK-EDVN-KMQLNPKSAAVILLRKILGRSKEGSLIAVAVLAE 300

Query: 301 NAIDSILGSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADEAHAHLASILGSFMEVSNE 360
           NAI+SIL SLKAKQVEERIAAVGILLRCIQEDGKCRN+IAD+  A LA ILGSFMEVSN+
Sbjct: 301 NAIESILVSLKAKQVEERIAAVGILLRCIQEDGKCRNMIADK--ADLAPILGSFMEVSND 360

Query: 361 EQFEIVMFLSDLVKLNRRTFNQQILQSIRDGGGCSTMHSLLIYLQTAHKDQSPVVAGLLL 420
           EQFEI+MFLS+LVKLNRRTFN+QILQ+I+DGG CSTMHSLL+YLQTA +DQ PVVAG LL
Sbjct: 361 EQFEIIMFLSELVKLNRRTFNEQILQNIKDGGECSTMHSLLVYLQTAPRDQCPVVAGFLL 420

Query: 421 QLDILVEPRKMSIYREEAMDVLFSCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYV 480
           QLDILVEPRK SIYREEAMDVL SCLG+SDFPTAQISAAETIMSLQGRFSTSGRPL RYV
Sbjct: 421 QLDILVEPRKSSIYREEAMDVLLSCLGNSDFPTAQISAAETIMSLQGRFSTSGRPLTRYV 480

Query: 481 LLERVGFMIGRMKLKPRGNNSNAPGQVKLTTE-EERANDEWERKMAFVLMSHDFGLVFEP 540
           LLERVGF  G MK K R NNS+ PG V+L+ + EERA DEWERKMAFVL SHDFGL+FEP
Sbjct: 481 LLERVGFAKGCMKPKRRDNNSSGPGDVELSIKAEERATDEWERKMAFVLASHDFGLLFEP 540

Query: 541 LAKGLKSKYAALFSACFVLATWLCHMLRVLPDTGILEAARVCLLDHFISIFTTTTDIEEK 600
           LAKGLKSKYAALFSACFV ATWL HML+VLPDTGIL+ ARVCLLDHF+SIF TTTDIEEK
Sbjct: 541 LAKGLKSKYAALFSACFVSATWLSHMLKVLPDTGILDTARVCLLDHFVSIFITTTDIEEK 600

Query: 601 ALGLLAMNSFVHEPEGMQCLRSNMKSINSGLKELRRSTPLAFEMLKVLCEGEESSTELWN 660
           AL LL MNSFVH+PEG+Q L SNMK I  GLKELRRST LAFEMLKVLC+GEESS ELW+
Sbjct: 601 ALALLGMNSFVHQPEGLQYLSSNMKDIMRGLKELRRSTALAFEMLKVLCKGEESSAELWS 660

Query: 661 HQELFQVDCSKNGEVLSIVYFKDKTISGHSDGTIKVWSVRGTNIHLLQETQEHSKGVTSL 720
           HQELF VDCS+NGEVLSI YFKDK ISGHSDGTIKVWSV GTN+H LQETQEHSKGVTSL
Sbjct: 661 HQELFLVDCSRNGEVLSIAYFKDKIISGHSDGTIKVWSVGGTNLHPLQETQEHSKGVTSL 720

Query: 721 AITESDDKLYSGSLDKTIKVWSLDSDKIQCIQVHDVKDQIHNLVVSKTVACFVPHGAGIR 780
           AI ES++KLYSGSLDKTIKVWSL SD IQCIQVHDVKDQ+HNLVVSKT+ACF+PHGAGIR
Sbjct: 721 AIIESEEKLYSGSLDKTIKVWSLGSDVIQCIQVHDVKDQVHNLVVSKTIACFIPHGAGIR 780

Query: 781 VYSWGGESKLLNSSKHVKCLDLVHGKLYCGCHDGSIQEVDLATETLSNIHSGSRKLLGKA 840
           VYSWGGESKLLNSSKHVK L+LV GKLYCGCHD SIQEVDLAT T+S IH GSRKLLGK 
Sbjct: 781 VYSWGGESKLLNSSKHVKRLNLVRGKLYCGCHDSSIQEVDLATGTVSYIHIGSRKLLGKP 840

Query: 841 PSVQALQIYDEQLFSACTTLDGSAVKIWNTSNNNVIASLSTPMDVRSMIVSSDLIYVGTR 900
             VQ+LQIYDEQLFSA TTLDG+AVKIW+ SNN+VIA+LST MD+R+M VSSDL Y+G R
Sbjct: 841 NIVQSLQIYDEQLFSASTTLDGAAVKIWSMSNNSVIATLSTAMDIRTMTVSSDLTYLGGR 900

Query: 901 -GVVEMWSREKYNKIDTLQTGRNCKVACMALSEREDVLVIGTSDGRIQAWGL 929
            GVVE+W R+K NKIDTLQTGR CKVACM L+EREDVLVIGTSDGRIQAWGL
Sbjct: 901 GGVVEIWGRDKLNKIDTLQTGRICKVACMTLNEREDVLVIGTSDGRIQAWGL 948

BLAST of Sed0019774 vs. ExPASy TrEMBL
Match: A0A5A7V5V1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79G001100 PE=4 SV=1)

HSP 1 Score: 1456.8 bits (3770), Expect = 0.0e+00
Identity = 751/951 (78.97%), Postives = 823/951 (86.54%), Query Frame = 0

Query: 1    MEDSGN---------NTTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTCP 60
            MEDSGN         NT PKDFVCPITCNIFYDPVT+ETGQTYER+AIQEWLDRGNSTCP
Sbjct: 469  MEDSGNASPESGERYNTPPKDFVCPITCNIFYDPVTLETGQTYERSAIQEWLDRGNSTCP 528

Query: 61   ITGQKLQNTQLPKTNYVLKRLIASWLEENPTFVSEKPFG-------LTSPVNVINRT--- 120
            ITGQKL+NTQLPKTNYVLKRLIASWLEENP F  +KP         LTSPV+VI++    
Sbjct: 529  ITGQKLENTQLPKTNYVLKRLIASWLEENPNFSLDKPIDEADPLAVLTSPVSVISQASID 588

Query: 121  ---MEVRQAITNLYLSEVLDDAEAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILV 180
                EVR+AI NLY SEVL++AEAAVLCVERFWLEENVEM IQ MLLKPPVINGLVEILV
Sbjct: 589  RGMKEVRRAIVNLYASEVLEEAEAAVLCVERFWLEENVEMDIQLMLLKPPVINGLVEILV 648

Query: 181  NSVNLHVLRASIFLLSELGFKDAAVIQMLTRVESDVDCILTLFKSGLMEAVVLIYQFGLS 240
            NSVN  VL A+IFLLSELGFKDAAVIQ L+RVESDVDCI+TLFK G MEAVVLIYQ GLS
Sbjct: 649  NSVNEQVLSAAIFLLSELGFKDAAVIQTLSRVESDVDCIVTLFKGGFMEAVVLIYQLGLS 708

Query: 241  VQHLQEMDLAGFLLNVVKKKGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAE 300
             Q LQEMD+ G LL  +KK   DV  KMRLS KSAAVILLRKILG+SKEGSLIAV VLAE
Sbjct: 709  SQSLQEMDIVGSLLAAIKKNEGDVK-KMRLSHKSAAVILLRKILGKSKEGSLIAVVVLAE 768

Query: 301  NAIDSILGSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADEAHAHLASILGSFMEVSNE 360
            NAI+ I+GSLKAKQVEERIAAVGILLRCIQEDG+CRNIIAD+  A LA +L SF+EVSN+
Sbjct: 769  NAIECIIGSLKAKQVEERIAAVGILLRCIQEDGRCRNIIADK--ADLALVLESFVEVSND 828

Query: 361  EQFEIVMFLSDLVKLNRRTFNQQILQSIRDGGGCSTMHSLLIYLQTAHKDQSPVVAGLLL 420
            EQFEI+ FLS+LVKLNRRTFN+QILQ+I+DGG  STMH LLIYLQTA +DQSPVVAGLLL
Sbjct: 829  EQFEIITFLSELVKLNRRTFNEQILQNIKDGGEYSTMHYLLIYLQTARRDQSPVVAGLLL 888

Query: 421  QLDILVEPRKMSIYREEAMDVLFSCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYV 480
            QLD+LVEPRKMSIYREEAMDVL SCLGDSDFPT QISAAETIMSLQGRFSTSGRPL RY 
Sbjct: 889  QLDVLVEPRKMSIYREEAMDVLISCLGDSDFPTTQISAAETIMSLQGRFSTSGRPLTRYF 948

Query: 481  LLERVGFMIGRMKLKPRGNNSNAPGQVKLTTEEERANDEWERKMAFVLMSHDFGLVFEPL 540
            LLER GF  G  K   R   S+APG+ +LT EEERA DEWERKMAFVL+SHDFGL+FEPL
Sbjct: 949  LLERAGFTKGHRKSIQRDTISSAPGEAELTREEERAADEWERKMAFVLISHDFGLLFEPL 1008

Query: 541  AKGLKSKYAALFSACFVLATWLCHMLRVLPDTGILEAARVCLLDHFISIFTTTTDIEEKA 600
            AKGLKSK+AALFSACFV ATWL HMLR LPDTGILE ARVCLLDHF+SIFTTTTD+EEK 
Sbjct: 1009 AKGLKSKFAALFSACFVSATWLSHMLRSLPDTGILETARVCLLDHFLSIFTTTTDVEEKT 1068

Query: 601  LGLLAMNSFVHEPEGMQCLRSNMKSINSGLKELRRSTPLAFEMLKVLCEGEESSTELWNH 660
            LGLLA+NSFVHEP+G+QCL SNMK I  GL+EL+RSTPLAFEMLKVLCE ++ S+E W+H
Sbjct: 1069 LGLLAINSFVHEPDGLQCLSSNMKDIMRGLRELKRSTPLAFEMLKVLCEEQDLSSEFWSH 1128

Query: 661  QELFQVDCSKNGEVLSIVYFKDKTISGHSDGTIKVWSVRGTNIHLLQETQEHSKGVTSLA 720
            QELFQVDCS NGEVLSI YFKDK ISGHSDG IKVWSVRGTN+HL+ E QEHSKGVTSLA
Sbjct: 1129 QELFQVDCSTNGEVLSIAYFKDKIISGHSDGRIKVWSVRGTNLHLIHEVQEHSKGVTSLA 1188

Query: 721  ITESDDKLYSGSLDKTIKVWSLDSDKIQCIQVHDVKDQIHNLVVSKTVACFVPHGAGIRV 780
            + E ++KLYSGSLDKTIKVWSL SD IQCIQ+HDVKDQIHNLVVSKTVACF+PHGAGIRV
Sbjct: 1189 VLEFEEKLYSGSLDKTIKVWSLGSDTIQCIQLHDVKDQIHNLVVSKTVACFIPHGAGIRV 1248

Query: 781  YSWGGESKLLNSSKHVKCLDLVHGKLYCGCHDGSIQEVDLATETLSNIHSGSRKLLGKAP 840
            YSWGGESKLLNSSKHVKCL+LV GKL+CGCHD SIQEVDLAT T++ IHSGSRKLLGKA 
Sbjct: 1249 YSWGGESKLLNSSKHVKCLNLVGGKLFCGCHDSSIQEVDLATGTINYIHSGSRKLLGKAN 1308

Query: 841  SVQALQIYDEQLFSACTTLDGSAVKIWNTSNNNVIASLSTPMDVRSMIVSSDLIYVGTR- 900
             +QALQ+YD+QLFSA T LDG+AVKIWNTS+  +I SL+T MDVRSM VSSDL Y+G + 
Sbjct: 1309 PIQALQVYDKQLFSASTALDGAAVKIWNTSDYGLIGSLTTSMDVRSMTVSSDLTYLGGKG 1368

Query: 901  GVVEMWSREKYNKIDTLQTGRNCKVACMALSEREDVLVIGTSDGRIQAWGL 929
            G+VE+W REK NK+DTLQ GRNCK+ CM L ERE+VLVIGTSDGRIQ WGL
Sbjct: 1369 GMVEIWCREKPNKVDTLQMGRNCKIICMTLDEREEVLVIGTSDGRIQGWGL 1416

BLAST of Sed0019774 vs. TAIR 10
Match: AT3G06880.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 211.8 bits (538), Expect = 2.3e-54
Identity = 202/843 (23.96%), Postives = 381/843 (45.20%), Query Frame = 0

Query: 101  TMEVRQAITNLYLSEVLDDA-EAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILVN 160
            TM++   + +L + EVL +A E  V  V   +   N +   +Y +LK  +++ L   + +
Sbjct: 438  TMDLWMNLQSL-IKEVLGNADEKYVSEVTMIYQMLNRKEGFKYSMLKDVILDQLFTAISS 497

Query: 161  SVNLHVLRASIFLLSELGFKDAAVIQMLTRVESDVDCILTLFKSGLMEAVVLIYQFGLSV 220
            S    V++AS+  L+++   +   ++ + R   ++  +    K  + EA +LIY    S 
Sbjct: 498  SEEKTVIKASMTALTKIISVNRTALEEVKRKGLNLSHLANALKQNVQEAAILIYLIKPSP 557

Query: 221  QHLQEMDLAGFLLNVVKKKGDD------VNDKMRLSQKSAAVILLRKILGR--SKEGSLI 280
              ++ ++L   L++VV            +     L+  +A+++++  ++        ++ 
Sbjct: 558  TEIKSLELLPALVDVVASTSSSSSCYTFIPSPSYLTPPAASLMIIEVLITAFDHATNTMH 617

Query: 281  AVAVLAENAIDSILGSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADEAHAHLASILGS 340
              A+ + + +  +L   K+    E I+   IL++C+Q DG  R  I    H  +A     
Sbjct: 618  LAAISSPSVLCGLLDVAKSGNSGEFISLTSILVKCMQFDGLHRKYIYQ--HTRVAPFAHL 677

Query: 341  FMEVSNEEQFEIVMFLSDLVKLNRRTFNQQILQSIRDGGGCSTMHSLLIYLQTAHKDQSP 400
                  EE    + FL +++K+ R +   +ILQ I+  G      +LL  ++    D   
Sbjct: 678  LQSKDQEEICIALQFLHEVLKIPRSS-AIKILQQIKKEGSFDIKVTLLHCIKHLQGDHKL 737

Query: 401  VVAGLLLQLDILVEPRKMSIYREEAMDVLFSCLGDSDFPTAQISAAETIMSLQGRFSTSG 460
              A +LLQL+ L  P +   YR EA   L   +  S+    Q+ +   + ++ G +S +G
Sbjct: 738  FAADILLQLNALDSPPENKKYRNEATRALLDAVTYSEGSNMQLLSTFILANIGGTYSWTG 797

Query: 461  RPLARYVLLERVGF-MIGRMKLKPRGNNSNAPGQVKLTTEEERANDEWERKMAFVLMSHD 520
             P     L++R G   +  M +    N S+          ++   D W  K+A  ++  D
Sbjct: 798  EPYTAAWLMKRGGLTSMSHMNMIRNINWSD-------ECLQDTGIDGWCCKIARRII--D 857

Query: 521  FG-LVFEPLAKGLKSKYAALFSACFVLATWLCHMLRVLPDTGILEAARVCLLDHFISIFT 580
             G   F  L +GLKSK  ++  AC +   WL   +   P++    A  V LLD       
Sbjct: 858  TGKATFCGLQEGLKSKNKSVSKACLIAIAWLSIEISKGPNSLKYSACEV-LLDEVAQFLH 917

Query: 581  TTTDIEEKALGLLAMNSFVHEPEGMQCLRSNMKSINSGLKELRRSTPLAFEMLKVLCEGE 640
               ++EE+ L  + + +F    +G+  L +  + +   L+ L   T +A E+ K      
Sbjct: 918  PGLELEERLLACICIYNF-SSGKGIHKLVNFSEGVRESLRRLSHVTWMADELHKATYYLF 977

Query: 641  ESSTELWN--HQELFQVDCSKNGEVLSIVYFKDKTISGHSDGTIKVWSVRGTNIHLLQET 700
              S +  +  H +  ++  S +G V +++Y K    SG SDG+I+VW+V      LL + 
Sbjct: 978  SKSDQRISCVHTQTVEMHQSGSGAVTALIYHKGLLFSGFSDGSIRVWNVNKKIATLLWDI 1037

Query: 701  QEHSKGVTSLAITESDDKLYSGSLDKTIKVWSLDSDKIQCIQVHDVKDQIHNLVVSKTVA 760
            +EH   VT  +++E+ + + SGS DKTI+VW +   K++C +V   KD I  L     + 
Sbjct: 1038 KEHKSTVTCFSLSETGECVLSGSADKTIRVWQIVKGKLECAEVIKTKDSIRKLEAFGNMI 1097

Query: 761  CFVPHGAGIRVYSWGGESKLLNSSKHVKCLDLVHGKLYCGCHDGSIQEVDLATETLSNIH 820
              +  G  +++      S+ +   K VK +    GK+Y GC D SIQE+ +A +    I 
Sbjct: 1098 FVITKGHKMKLLDSSRISQSIFKGKGVKSMVSAQGKIYIGCIDTSIQELIVANKREKEIK 1157

Query: 821  SGSRKLLGKAPSVQALQIYDEQLFSACTTLDGSAVK-IWNTSNNNVIASLSTPMDVRSMI 880
            + +R    +   + ++ +Y + L+S+ T ++ S +K +       +  +     ++ +M 
Sbjct: 1158 APTRSWRLQNKPINSVVVYKDMLYSSSTYVEMSNIKDLRRNYEPQMSITAEKGSNIVAMG 1217

Query: 881  VSSDLIYV---GTRGVVEMWSREKYNKIDTLQTGRNCKVACMALSEREDVLVIGTSDGRI 927
            V  D IY+    +   +++W R    K+  L  G        +L    D++  GT  G I
Sbjct: 1218 VVEDFIYLNRSSSANTLQIWLRRTQQKVGRLSAGSK----ITSLLTANDIVFCGTEAGVI 1261

BLAST of Sed0019774 vs. TAIR 10
Match: AT3G06880.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 206.1 bits (523), Expect = 1.2e-52
Identity = 200/838 (23.87%), Postives = 378/838 (45.11%), Query Frame = 0

Query: 101  TMEVRQAITNLYLSEVLDDA-EAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILVN 160
            TM++   + +L + EVL +A E  V  V   +   N +   +Y +LK  +++ L   + +
Sbjct: 438  TMDLWMNLQSL-IKEVLGNADEKYVSEVTMIYQMLNRKEGFKYSMLKDVILDQLFTAISS 497

Query: 161  SVNLHVLRASIFLLSELGFKDAAVIQMLTRVESDVDCILTLFKSGLMEAVVLIYQFGLSV 220
            S    V++AS+  L+++   +   ++ + R   ++  +    K  + EA +LIY    S 
Sbjct: 498  SEEKTVIKASMTALTKIISVNRTALEEVKRKGLNLSHLANALKQNVQEAAILIYLIKPSP 557

Query: 221  QHLQEMDLAGFLLNVVKKKGDD------VNDKMRLSQKSAAVILLRKILGR--SKEGSLI 280
              ++ ++L   L++VV            +     L+  +A+++++  ++        ++ 
Sbjct: 558  TEIKSLELLPALVDVVASTSSSSSCYTFIPSPSYLTPPAASLMIIEVLITAFDHATNTMH 617

Query: 281  AVAVLAENAIDSILGSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADEAHAHLASILGS 340
              A+ + + +  +L   K+    E I+   IL++C+Q DG  R  I    H  +A     
Sbjct: 618  LAAISSPSVLCGLLDVAKSGNSGEFISLTSILVKCMQFDGLHRKYIYQ--HTRVAPFAHL 677

Query: 341  FMEVSNEEQFEIVMFLSDLVKLNRRTFNQQILQSIRDGGGCSTMHSLLIYLQTAHKDQSP 400
                  EE    + FL +++K+ R +   +ILQ I+  G      +LL  ++    D   
Sbjct: 678  LQSKDQEEICIALQFLHEVLKIPRSS-AIKILQQIKKEGSFDIKVTLLHCIKHLQGDHKL 737

Query: 401  VVAGLLLQLDILVEPRKMSIYREEAMDVLFSCLGDSDFPTAQISAAETIMSLQGRFSTSG 460
              A +LLQL+ L  P +   YR EA   L   +  S+    Q+ +   + ++ G +S +G
Sbjct: 738  FAADILLQLNALDSPPENKKYRNEATRALLDAVTYSEGSNMQLLSTFILANIGGTYSWTG 797

Query: 461  RPLARYVLLERVGF-MIGRMKLKPRGNNSNAPGQVKLTTEEERANDEWERKMAFVLMSHD 520
             P     L++R G   +  M +    N S+          ++   D W  K+A  ++  D
Sbjct: 798  EPYTAAWLMKRGGLTSMSHMNMIRNINWSD-------ECLQDTGIDGWCCKIARRII--D 857

Query: 521  FG-LVFEPLAKGLKSKYAALFSACFVLATWLCHMLRVLPDTGILEAARVCLLDHFISIFT 580
             G   F  L +GLKSK  ++  AC +   WL   +   P++    A  V LLD       
Sbjct: 858  TGKATFCGLQEGLKSKNKSVSKACLIAIAWLSIEISKGPNSLKYSACEV-LLDEVAQFLH 917

Query: 581  TTTDIEEKALGLLAMNSFVHEPEGMQCLRSNMKSINSGLKELRRSTPLAFEMLKVLCEGE 640
               ++EE+ L  + + +F    +G+  L +  + +   L+ L   T +A E+ K      
Sbjct: 918  PGLELEERLLACICIYNF-SSGKGIHKLVNFSEGVRESLRRLSHVTWMADELHKATYYLF 977

Query: 641  ESSTELWN--HQELFQVDCSKNGEVLSIVYFKDKTISGHSDGTIKVWSVRGTNIHLLQET 700
              S +  +  H +  ++  S +G V +++Y K    SG SDG+I+VW+V      LL + 
Sbjct: 978  SKSDQRISCVHTQTVEMHQSGSGAVTALIYHKGLLFSGFSDGSIRVWNVNKKIATLLWDI 1037

Query: 701  QEHSKGVTSLAITESDDKLYSGSLDKTIKVWSLDSDKIQCIQVHDVKDQIHNLVVSKTVA 760
            +EH   VT  +++E+ + + SGS DKTI+VW +   K++C +V   KD I  L     + 
Sbjct: 1038 KEHKSTVTCFSLSETGECVLSGSADKTIRVWQIVKGKLECAEVIKTKDSIRKLEAFGNMI 1097

Query: 761  CFVPHGAGIRVYSWGGESKLLNSSKHVKCLDLVHGKLYCGCHDGSIQEVDLATETLSNIH 820
              +  G  +++      S+ +   K VK +    GK+Y GC D SIQE+ +A +    I 
Sbjct: 1098 FVITKGHKMKLLDSSRISQSIFKGKGVKSMVSAQGKIYIGCIDTSIQELIVANKREKEIK 1157

Query: 821  SGSRKLLGKAPSVQALQIYDEQLFSACTTLDGSAVK-IWNTSNNNVIASLSTPMDVRSMI 880
            + +R    +   + ++ +Y + L+S+ T ++ S +K +       +  +     ++ +M 
Sbjct: 1158 APTRSWRLQNKPINSVVVYKDMLYSSSTYVEMSNIKDLRRNYEPQMSITAEKGSNIVAMG 1217

Query: 881  VSSDLIYV---GTRGVVEMWSREKYNKIDTLQTGRNCKVACMALSEREDVLVIGTSDG 922
            V  D IY+    +   +++W R    K+  L  G        +L    D++  GT  G
Sbjct: 1218 VVEDFIYLNRSSSANTLQIWLRRTQQKVGRLSAGSK----ITSLLTANDIVFCGTEAG 1256

BLAST of Sed0019774 vs. TAIR 10
Match: AT1G29340.1 (plant U-box 17 )

HSP 1 Score: 83.6 bits (205), Expect = 9.3e-16
Identity = 84/320 (26.25%), Postives = 144/320 (45.00%), Query Frame = 0

Query: 8   TTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTCPITGQKLQNTQLPKTNY 67
           T PKDFVCPI+ ++  DPV I TGQTY+RN+I  W++ G+ TCP TGQ L ++++   N 
Sbjct: 304 TVPKDFVCPISLDLMTDPVIISTGQTYDRNSIARWIEEGHCTCPKTGQMLMDSRI-VPNR 363

Query: 68  VLKRLIASW-------LEENPTFVSEKPFGLTSPVNVINRTMEVRQAITNLYLSEVLDDA 127
            LK LI  W        E   T    + F    P       +E  +A  ++ +  + D +
Sbjct: 364 ALKNLIVQWCTASGISYESEFTDSPNESFASALPTKA---AVEANKATVSILIKYLADGS 423

Query: 128 EAA-VLCVERFWLEENVEMKIQYMLLKPPVINGLVEILVNSVNLHVLRASIFLLSELGFK 187
           +AA  +      L      + +  + +   I  L  +L  S N      S+  +  L   
Sbjct: 424 QAAQTVAAREIRLLAKTGKENRAYIAEAGAIPHLCRLL-TSENAIAQENSVTAMLNLSIY 483

Query: 188 DAAVIQMLTRVESDVDCILTLFKSGLMEAVVLIYQFGLSVQHLQEMDLAGFLLNVVK--K 247
           +    +  +R+  + DC         +E++V +   GL+V+  +      F L+ V   K
Sbjct: 484 E----KNKSRIMEEGDC---------LESIVSVLVSGLTVEAQENAAATLFSLSAVHEYK 543

Query: 248 KGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAEN-----------AIDSILG 306
           K   + D+       A  +LL+    R K+ ++ A+  L+ +            + S++G
Sbjct: 544 KRIAIVDQC----VEALALLLQNGTPRGKKDAVTALYNLSTHPDNCSRMIEGGGVSSLVG 601

BLAST of Sed0019774 vs. TAIR 10
Match: AT1G71020.1 (ARM repeat superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 2.7e-15
Identity = 72/311 (23.15%), Postives = 145/311 (46.62%), Query Frame = 0

Query: 2   EDSGNNTTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTCPITGQKLQNTQ 61
           + S N T P+DF+CPI+  +  DP  + TGQTYER+ IQ W+D GN +CP T QKL+N  
Sbjct: 236 QKSDNLTIPEDFLCPISLELMKDPAIVSTGQTYERSFIQRWIDCGNLSCPKTQQKLENFT 295

Query: 62  LPKTNYVLKRLIASW-----LEENPTFVSEKPFGLTSPVNVINRTMEVRQAITNLYLSEV 121
           L   NYVL+ LI+ W     +E+   +++ +          ++  M   +A+     S+ 
Sbjct: 296 L-TPNYVLRSLISQWCTKHNIEQPGGYMNGRTKNSDGSFRDLSGDMSAIRALVCKLSSQS 355

Query: 122 LDDAEAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILVNSVNLHVLRASIFLLSEL 181
           ++D   AV  + R   + + + +I  ++ +   I  LV++L +  +      ++  +  L
Sbjct: 356 IEDRRTAVSEI-RSLSKRSTDNRI--LIAEAGAIPVLVKLLTSDGDTETQENAVTCILNL 415

Query: 182 GFKDAAVIQMLTRVESDVDCILTLFKSGLME----AVVLIYQFGLSVQHLQEMDLAGFLL 241
              +    + L  +   V  I+ + ++G ME    A   ++   L+ ++   +  +G ++
Sbjct: 416 SIYEHN--KELIMLAGAVTSIVLVLRAGSMEARENAAATLFSLSLADENKIIIGASGAIM 475

Query: 242 NVVKKKGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAENAIDSILGSLKAKQ 301
            +V    D +       +K AA  L    + +  +G  +   ++ +  +  +  S   + 
Sbjct: 476 ALV----DLLQYGSVRGKKDAATALFNLCIYQGNKGRAVRAGIV-KPLVKMLTDSSSERM 535

Query: 302 VEERIAAVGIL 304
            +E +  + +L
Sbjct: 536 ADEALTILSVL 535

BLAST of Sed0019774 vs. TAIR 10
Match: AT1G71020.2 (ARM repeat superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 2.7e-15
Identity = 72/311 (23.15%), Postives = 145/311 (46.62%), Query Frame = 0

Query: 2   EDSGNNTTPKDFVCPITCNIFYDPVTIETGQTYERNAIQEWLDRGNSTCPITGQKLQNTQ 61
           + S N T P+DF+CPI+  +  DP  + TGQTYER+ IQ W+D GN +CP T QKL+N  
Sbjct: 88  QKSDNLTIPEDFLCPISLELMKDPAIVSTGQTYERSFIQRWIDCGNLSCPKTQQKLENFT 147

Query: 62  LPKTNYVLKRLIASW-----LEENPTFVSEKPFGLTSPVNVINRTMEVRQAITNLYLSEV 121
           L   NYVL+ LI+ W     +E+   +++ +          ++  M   +A+     S+ 
Sbjct: 148 L-TPNYVLRSLISQWCTKHNIEQPGGYMNGRTKNSDGSFRDLSGDMSAIRALVCKLSSQS 207

Query: 122 LDDAEAAVLCVERFWLEENVEMKIQYMLLKPPVINGLVEILVNSVNLHVLRASIFLLSEL 181
           ++D   AV  + R   + + + +I  ++ +   I  LV++L +  +      ++  +  L
Sbjct: 208 IEDRRTAVSEI-RSLSKRSTDNRI--LIAEAGAIPVLVKLLTSDGDTETQENAVTCILNL 267

Query: 182 GFKDAAVIQMLTRVESDVDCILTLFKSGLME----AVVLIYQFGLSVQHLQEMDLAGFLL 241
              +    + L  +   V  I+ + ++G ME    A   ++   L+ ++   +  +G ++
Sbjct: 268 SIYEHN--KELIMLAGAVTSIVLVLRAGSMEARENAAATLFSLSLADENKIIIGASGAIM 327

Query: 242 NVVKKKGDDVNDKMRLSQKSAAVILLRKILGRSKEGSLIAVAVLAENAIDSILGSLKAKQ 301
            +V    D +       +K AA  L    + +  +G  +   ++ +  +  +  S   + 
Sbjct: 328 ALV----DLLQYGSVRGKKDAATALFNLCIYQGNKGRAVRAGIV-KPLVKMLTDSSSERM 387

Query: 302 VEERIAAVGIL 304
            +E +  + +L
Sbjct: 388 ADEALTILSVL 387