Sed0019527 (gene) Chayote v1

Overview


Name	Sed0019527
Type	gene
Organism	Sechium edule (Chayote v1)
Description	E3 ubiquitin-protein ligase RMA1H1-like
Location	LG01: 15719773 .. 15720420 (-)
RNA-Seq Expression	Sed0019527
Synteny	Sed0019527

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTCAAGTTTAAGGCCTGCTTCGACTGCAACATTTGCCTAGATTTTGCATTTGAGCCTATAGTTACCCTGTGTGGCCACCTCTATTGCTGGCCTTGCATATACAAGTGGCTTCGTGTCCACAGCGCCTCACTCGCCCCCGAAGAGCCTCCACAGTGCCCAGTTTGCAAGTCCGATATTAACCATACGACAATGATCCCTCTCTATGGCCGTGGTCAATCAGCGGAAGATGCTGAGGTTGATGCCAAAGGGCTGCTCATACCTCCTCGACCATCAGCTTTCGGTACTAAAGATCTGGCACCTATTAGATCTGATAATGCTTATGGTGACCTATATCAGAATGAAAACTACAATGATGATCGGTATGACAGTTATGACGAAGACGAAGATTCCAGCAGCTCATCATTTAACCTCGAACACAACCCGGTCAATAGTTTTCACCATCCTACAGCAAGGATGTTCGGGGAGGTGTATGGTAGGTTGTTTGGGGATTCAGAAAACTTATATGTACGCCCGAATTCTTATGCTCTAACTGGAAATAACAGTCCTCGGTCGAGAAGGCGTGAAACTCTGGCCGAGCAGTCACTTAAGAGGATGTCATTATGTCTCTTCTATTTCTTTGTTGTATGCCTTGCTCTCTTCTAG

mRNA sequence

ATGGCATTCAAGTTTAAGGCCTGCTTCGACTGCAACATTTGCCTAGATTTTGCATTTGAGCCTATAGTTACCCTGTGTGGCCACCTCTATTGCTGGCCTTGCATATACAAGTGGCTTCGTGTCCACAGCGCCTCACTCGCCCCCGAAGAGCCTCCACAGTGCCCAGTTTGCAAGTCCGATATTAACCATACGACAATGATCCCTCTCTATGGCCGTGGTCAATCAGCGGAAGATGCTGAGGTTGATGCCAAAGGGCTGCTCATACCTCCTCGACCATCAGCTTTCGGTACTAAAGATCTGGCACCTATTAGATCTGATAATGCTTATGGTGACCTATATCAGAATGAAAACTACAATGATGATCGGTATGACAGTTATGACGAAGACGAAGATTCCAGCAGCTCATCATTTAACCTCGAACACAACCCGGTCAATAGTTTTCACCATCCTACAGCAAGGATGTTCGGGGAGGTGTATGGTAGGTTGTTTGGGGATTCAGAAAACTTATATGTACGCCCGAATTCTTATGCTCTAACTGGAAATAACAGTCCTCGGTCGAGAAGGCGTGAAACTCTGGCCGAGCAGTCACTTAAGAGGATGTCATTATGTCTCTTCTATTTCTTTGTTGTATGCCTTGCTCTCTTCTAG

Coding sequence (CDS)

ATGGCATTCAAGTTTAAGGCCTGCTTCGACTGCAACATTTGCCTAGATTTTGCATTTGAGCCTATAGTTACCCTGTGTGGCCACCTCTATTGCTGGCCTTGCATATACAAGTGGCTTCGTGTCCACAGCGCCTCACTCGCCCCCGAAGAGCCTCCACAGTGCCCAGTTTGCAAGTCCGATATTAACCATACGACAATGATCCCTCTCTATGGCCGTGGTCAATCAGCGGAAGATGCTGAGGTTGATGCCAAAGGGCTGCTCATACCTCCTCGACCATCAGCTTTCGGTACTAAAGATCTGGCACCTATTAGATCTGATAATGCTTATGGTGACCTATATCAGAATGAAAACTACAATGATGATCGGTATGACAGTTATGACGAAGACGAAGATTCCAGCAGCTCATCATTTAACCTCGAACACAACCCGGTCAATAGTTTTCACCATCCTACAGCAAGGATGTTCGGGGAGGTGTATGGTAGGTTGTTTGGGGATTCAGAAAACTTATATGTACGCCCGAATTCTTATGCTCTAACTGGAAATAACAGTCCTCGGTCGAGAAGGCGTGAAACTCTGGCCGAGCAGTCACTTAAGAGGATGTCATTATGTCTCTTCTATTTCTTTGTTGTATGCCTTGCTCTCTTCTAG

Protein sequence

MAFKFKACFDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEEPPQCPVCKSDINHTTMIPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDNAYGDLYQNENYNDDRYDSYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGEVYGRLFGDSENLYVRPNSYALTGNNSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF

Homology

[Close]

BLAST of Sed0019527 vs. NCBI nr
Match: XP_023519102.1 (E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo] >XP_023519103.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo] >XP_023519104.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 315.8 bits (808), Expect = 2.8e-82
Identity = 160/219 (73.06%), Postives = 180/219 (82.19%), Query Frame = 0


Query: 4   KFKACFDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEEPPQCPVCKSDINH 63
           KFKACFDCNICL+FA EPIVTLCGHLYCWPCIYKWL V SASL P+EPPQCPVCK+DI+H
Sbjct: 25  KFKACFDCNICLEFASEPIVTLCGHLYCWPCIYKWLHVQSASLLPDEPPQCPVCKADISH 84

Query: 64  TTMIPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDNA------YGDLYQNEN 123
           TTM+PLYGRGQSA++AEVD +GLLIPPRPSAFG K LA I S+NA      YGDLYQN+N
Sbjct: 85  TTMVPLYGRGQSAKEAEVDPRGLLIPPRPSAFGNKVLASIGSNNASGEQLVYGDLYQNQN 144

Query: 124 YNDDRYDSYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGE-VYGRLFGDSENLYVRPNSY 183
            N D YDSY  +EDSSSS FN+E NPV SF HPTARMFGE VYGRLFG SENLY  PNSY
Sbjct: 145 DNFDMYDSY--EEDSSSSLFNMEDNPVGSFGHPTARMFGETVYGRLFGSSENLYAHPNSY 204

Query: 184 ALTGNNSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
           ALTG+ SP+ R    + E+SL R+S+ LF  FV+CLA+F
Sbjct: 205 ALTGSGSPQLR----MQEKSLNRISIFLFCCFVLCLAIF 237

[Close]

BLAST of Sed0019527 vs. NCBI nr
Match: XP_008442466.1 (PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >XP_008442474.1 PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >XP_008442488.1 PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >KAA0056985.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo var. makuwa] >TYK26413.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo var. makuwa])

HSP 1 Score: 315.8 bits (808), Expect = 2.8e-82
Identity = 158/219 (72.15%), Postives = 179/219 (81.74%), Query Frame = 0


Query: 4   KFKACFDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEEPPQCPVCKSDINH 63
           KFK CFDCNICLDFA EPIVTLCGHLYCWPCIYKWL V SASLAP+EPPQCPVCK+DI+H
Sbjct: 24  KFKPCFDCNICLDFASEPIVTLCGHLYCWPCIYKWLHVQSASLAPDEPPQCPVCKADISH 83

Query: 64  TTMIPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDN------AYGDLYQNEN 123
           TTM+PLYGRGQSAE AE DA+G LIPPRPSAFG K LA IRS+N      AYGDL QN+N
Sbjct: 84  TTMVPLYGRGQSAEHAEDDARGTLIPPRPSAFGNKALATIRSNNASSQQFAYGDLDQNQN 143

Query: 124 YNDDRYDSYDEDEDSSSSSFNLEHNPVNSFHHPTARMF-GEVYGRLFGDSENLYVRPNSY 183
           YN D YDSY  +EDS SS F+LE NPV S HHPTA +  G VYGRL+GDSEN+Y  PNSY
Sbjct: 144 YNFDVYDSY--EEDSGSSLFSLEDNPVGSSHHPTAGLLGGTVYGRLYGDSENVYRHPNSY 203

Query: 184 ALTGNNSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
            L+G+NSPR RR+E +AE+SL R+S  LF  F++CLA+F
Sbjct: 204 GLSGSNSPRLRRQEIVAEKSLNRISFFLFCCFILCLAVF 240

[Close]

BLAST of Sed0019527 vs. NCBI nr
Match: KAG7019622.1 (E3 ubiquitin-protein ligase RMA1H1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 313.5 bits (802), Expect = 1.4e-81
Identity = 159/219 (72.60%), Postives = 179/219 (81.74%), Query Frame = 0


Query: 4   KFKACFDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEEPPQCPVCKSDINH 63
           KFKACFDCNICL+FA EPIVTLCGHLYCWPCIYKWL V SASL P+EPPQCPVCK+DI+H
Sbjct: 126 KFKACFDCNICLEFASEPIVTLCGHLYCWPCIYKWLHVQSASLLPDEPPQCPVCKADISH 185

Query: 64  TTMIPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDNA------YGDLYQNEN 123
           TTM+PLYGRGQSA++AEVD +GLLIPPRPSA G K LA I S+NA      YGDLYQN+N
Sbjct: 186 TTMVPLYGRGQSAKEAEVDPRGLLIPPRPSALGNKVLASIGSNNASGEQLVYGDLYQNQN 245

Query: 124 YNDDRYDSYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGE-VYGRLFGDSENLYVRPNSY 183
            N D YDSY  +EDSSSS FN+E NPV SF HPTARMFGE VYGRLFG SENLY  PNSY
Sbjct: 246 DNFDMYDSY--EEDSSSSLFNMEDNPVGSFGHPTARMFGETVYGRLFGSSENLYAHPNSY 305

Query: 184 ALTGNNSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
           ALTG+ SP+ R    + E+SL R+S+ LF  FV+CLA+F
Sbjct: 306 ALTGSGSPQLR----MQEKSLNRISIFLFCCFVLCLAIF 338

[Close]

BLAST of Sed0019527 vs. NCBI nr
Match: KAG6584000.1 (E3 ubiquitin-protein ligase RMA1H1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 313.5 bits (802), Expect = 1.4e-81
Identity = 159/219 (72.60%), Postives = 179/219 (81.74%), Query Frame = 0


Query: 4   KFKACFDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEEPPQCPVCKSDINH 63
           KFKACFDCNICL+FA EPIVTLCGHLYCWPCIYKWL V SASL P+EPPQCPVCK+DI+H
Sbjct: 67  KFKACFDCNICLEFASEPIVTLCGHLYCWPCIYKWLHVQSASLLPDEPPQCPVCKADISH 126

Query: 64  TTMIPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDNA------YGDLYQNEN 123
           TTM+PLYGRGQSA++AEVD +GLLIPPRPSA G K LA I S+NA      YGDLYQN+N
Sbjct: 127 TTMVPLYGRGQSAKEAEVDPRGLLIPPRPSALGNKVLASIGSNNASGEQLVYGDLYQNQN 186

Query: 124 YNDDRYDSYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGE-VYGRLFGDSENLYVRPNSY 183
            N D YDSY  +EDSSSS FN+E NPV SF HPTARMFGE VYGRLFG SENLY  PNSY
Sbjct: 187 DNFDMYDSY--EEDSSSSLFNMEDNPVGSFGHPTARMFGETVYGRLFGSSENLYAHPNSY 246

Query: 184 ALTGNNSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
           ALTG+ SP+ R    + E+SL R+S+ LF  FV+CLA+F
Sbjct: 247 ALTGSGSPQLR----MQEKSLNRISIFLFCCFVLCLAIF 279

[Close]

BLAST of Sed0019527 vs. NCBI nr
Match: XP_038895713.1 (E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida])

HSP 1 Score: 313.2 bits (801), Expect = 1.8e-81
Identity = 157/219 (71.69%), Postives = 178/219 (81.28%), Query Frame = 0


Query: 4   KFKACFDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEEPPQCPVCKSDINH 63
           KFK CFDCNICLDFA EPIVTLCGHLYCWPCIYKWL V SASL P+EPPQCPVCK++I+H
Sbjct: 24  KFKPCFDCNICLDFASEPIVTLCGHLYCWPCIYKWLHVQSASLTPDEPPQCPVCKANISH 83

Query: 64  TTMIPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDN------AYGDLYQNEN 123
           TTM+PLYGRGQSAEDAEVDA+GLLIPPRPSAFG K LA IRS+       AYGDLYQN+ 
Sbjct: 84  TTMVPLYGRGQSAEDAEVDARGLLIPPRPSAFGNKALATIRSNTASGQQFAYGDLYQNQI 143

Query: 124 YNDDRYDSYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGE-VYGRLFGDSENLYVRPNSY 183
           YN D YDSY  +EDSSSS FNLE + V S HHPTA MF E VY R FG+SEN+Y  P+SY
Sbjct: 144 YNFDMYDSY--EEDSSSSLFNLEDSSVGSLHHPTAGMFTETVYARFFGNSENVYTHPSSY 203

Query: 184 ALTGNNSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
             +GN+SPR RR+E LAE+SL R+S+ LF  FV+CL +F
Sbjct: 204 GPSGNSSPRLRRQEILAEKSLNRISIFLFCCFVLCLVVF 240

[Close]

BLAST of Sed0019527 vs. ExPASy Swiss-Prot
Match: Q6R567 (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.0e-34
Identity = 96/218 (44.04%), Postives = 123/218 (56.42%), Query Frame = 0


Query: 9   FDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASL--APEEPPQCPVCKSDINHTTM 68
           FDCNICLD   EP++TLCGHLYCWPCIYKW+   S S   + ++ PQCPVCK++++  T+
Sbjct: 39  FDCNICLDCVHEPVITLCGHLYCWPCIYKWIYFQSVSSENSDQQQPQCPVCKAEVSEKTL 98

Query: 69  IPLYGR-GQSAEDAEVDAK--GLLIPPRPSA--FGTKDLAPIRSDNAYGDLYQNENYNDD 128
           IPLYGR GQS + +E  A   G++IP RP +   G   L P    N    L Q   Y   
Sbjct: 99  IPLYGRGGQSTKPSEGKAPNLGIVIPQRPPSPRCGGHFLLPTTDSNP-SQLLQRRGYQQQ 158

Query: 129 RYDSYDEDEDSSSSSFNLEHN--PVNSFHHPTARMFGEV-YGRLFGDSE-NLYVRPNSYA 188
                   + S  SS  L       N   H    M GEV Y R+FG+S   +Y  PNSY 
Sbjct: 159 SQTRQPAYQGSYMSSPMLSPGGATANMLQH---SMIGEVAYARIFGNSSTTMYTYPNSYN 218

Query: 189 LTGNNSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
           L  ++SPR RR+ + A++SL R+   LF  FV CL LF
Sbjct: 219 LAISSSPRMRRQLSQADRSLGRICFFLFCCFVTCLILF 252

[Close]

BLAST of Sed0019527 vs. ExPASy Swiss-Prot
Match: Q8GUK7 (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 6.3e-29
Identity = 82/216 (37.96%), Postives = 123/216 (56.94%), Query Frame = 0


Query: 8   CFDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEE-PPQCPVCKSDINHTTM 67
           CFDCNICLD A +P+VTLCGHL+CWPCIYKWL V  +S++ ++    CPVCKS+I  T++
Sbjct: 41  CFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNITITSL 100

Query: 68  IPLYGRGQSAEDA-----EVDAKGLLIPPRPSAFGTKDLAPIRSDNAYGDLYQNENYNDD 127
           +PLYGRG S+  +     + DA    IP RP+    ++  PI S ++     Q++  +  
Sbjct: 101 VPLYGRGMSSPSSTFGSKKQDALSTDIPRRPAPSALRN--PITSASSLNPSLQHQTLSPS 160

Query: 128 RYD-SYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGE-VYGRLFGDSENLYVRPNSYALT 187
            ++  Y     +++ S +L +  + SF +P   MFG+ VY R+FG   N   +P      
Sbjct: 161 FHNHQYSPRGFTTTESTDLANAVMMSFLYPVIGMFGDLVYTRIFGTFTNTIAQP------ 220

Query: 188 GNNSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
              S R  +R    E+SL R+S+      ++CL LF
Sbjct: 221 -YQSQRMMQR----EKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of Sed0019527 vs. ExPASy Swiss-Prot
Match: O64425 (E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.6e-27
Identity = 86/212 (40.57%), Postives = 115/212 (54.25%), Query Frame = 0


Query: 9   FDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPE--EPPQCPVCKSDINHTTM 68
           FDCNICLD   EP+VTLCGHL+CWPCI+KWL V S S + E     QCPVCKS ++H+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 69  IPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDNAYGD--LYQNENYNDDRYD 128
           +PLYGRG+     E       +P RP       L    S  A  D  L Q  ++N  +  
Sbjct: 106 VPLYGRGRCTTQEEGKNS---VPKRPVG-PVYRLEMPNSPYASTDLRLSQRVHFNSPQEG 165

Query: 129 SYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGE-VYGRLFGDS-ENLYVRPNSYALTGNN 188
            Y      SS+S +      ++   P   M GE V  RLFG    + +  P++Y L G +
Sbjct: 166 YYPVSGVMSSNSLS-----YSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLAGTS 225

Query: 189 SPRSRRRETLAEQSLKRMSLCLFYFFVVCLAL 215
            PR RRR   A++SL R    +F+FF+ C+ L
Sbjct: 226 GPRMRRRIMQADKSLGR----IFFFFMCCVVL 244

[Close]

BLAST of Sed0019527 vs. ExPASy Swiss-Prot
Match: P93030 (E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.0e-23
Identity = 79/214 (36.92%), Postives = 104/214 (48.60%), Query Frame = 0


Query: 9   FDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLA-------PEEPPQCPVCKSDI 68
           FDCNICLD   +P+VTLCGHL+CWPCI+KW    + S           EPP+CPVCKSD+
Sbjct: 19  FDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKSDV 78

Query: 69  NHTTMIPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDNAYGDLYQNENYNDD 128
           +  T++P+YGRGQ A  +     G  +P RP+                G +Y      D 
Sbjct: 79  SEATLVPIYGRGQKAPQS-----GSNVPSRPT----------------GPVY------DL 138

Query: 129 RYDSYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGEVYGRLFGDSENLYVRPNSYALTGN 188
           R       E  S        +PV         +   VY RLFG+S       ++ A   +
Sbjct: 139 RGVGQRLGEGESQRYMYRMPDPVMGV------VCEMVYRRLFGESS------SNMAPYRD 193

Query: 189 NSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
            + RSRRR   AE+SL R+ L L  F  +CL LF
Sbjct: 199 MNVRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of Sed0019527 vs. ExPASy Swiss-Prot
Match: Q99942 (E3 ubiquitin-protein ligase RNF5 OS=Homo sapiens OX=9606 GN=RNF5 PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 5.5e-17
Identity = 41/87 (47.13%), Postives = 55/87 (63.22%), Query Frame = 0


Query: 7  ACFDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEEPPQCPVCKSDINHTTM 66
          A F+CNICL+ A E +V++CGHLYCWPC+++WL          E  +CPVCK+ I+   +
Sbjct: 23 ATFECNICLETAREAVVSVCGHLYCWPCLHQWLETR------PERQECPVCKAGISREKV 82

Query: 67 IPLYGRG-QSAEDAEVDAKGLLIPPRP 93
          +PLYGRG Q  +D       L  PPRP
Sbjct: 83 VPLYGRGSQKPQDPR-----LKTPPRP 98

[Close]

BLAST of Sed0019527 vs. ExPASy TrEMBL
Match: A0A1S3B6D8 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo OX=3656 GN=LOC103486320 PE=4 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 1.3e-82
Identity = 158/219 (72.15%), Postives = 179/219 (81.74%), Query Frame = 0


Query: 4   KFKACFDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEEPPQCPVCKSDINH 63
           KFK CFDCNICLDFA EPIVTLCGHLYCWPCIYKWL V SASLAP+EPPQCPVCK+DI+H
Sbjct: 24  KFKPCFDCNICLDFASEPIVTLCGHLYCWPCIYKWLHVQSASLAPDEPPQCPVCKADISH 83

Query: 64  TTMIPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDN------AYGDLYQNEN 123
           TTM+PLYGRGQSAE AE DA+G LIPPRPSAFG K LA IRS+N      AYGDL QN+N
Sbjct: 84  TTMVPLYGRGQSAEHAEDDARGTLIPPRPSAFGNKALATIRSNNASSQQFAYGDLDQNQN 143

Query: 124 YNDDRYDSYDEDEDSSSSSFNLEHNPVNSFHHPTARMF-GEVYGRLFGDSENLYVRPNSY 183
           YN D YDSY  +EDS SS F+LE NPV S HHPTA +  G VYGRL+GDSEN+Y  PNSY
Sbjct: 144 YNFDVYDSY--EEDSGSSLFSLEDNPVGSSHHPTAGLLGGTVYGRLYGDSENVYRHPNSY 203

Query: 184 ALTGNNSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
            L+G+NSPR RR+E +AE+SL R+S  LF  F++CLA+F
Sbjct: 204 GLSGSNSPRLRRQEIVAEKSLNRISFFLFCCFILCLAVF 240

[Close]

BLAST of Sed0019527 vs. ExPASy TrEMBL
Match: A0A5A7UTV8 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00770 PE=4 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 1.3e-82
Identity = 158/219 (72.15%), Postives = 179/219 (81.74%), Query Frame = 0


Query: 4   KFKACFDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEEPPQCPVCKSDINH 63
           KFK CFDCNICLDFA EPIVTLCGHLYCWPCIYKWL V SASLAP+EPPQCPVCK+DI+H
Sbjct: 24  KFKPCFDCNICLDFASEPIVTLCGHLYCWPCIYKWLHVQSASLAPDEPPQCPVCKADISH 83

Query: 64  TTMIPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDN------AYGDLYQNEN 123
           TTM+PLYGRGQSAE AE DA+G LIPPRPSAFG K LA IRS+N      AYGDL QN+N
Sbjct: 84  TTMVPLYGRGQSAEHAEDDARGTLIPPRPSAFGNKALATIRSNNASSQQFAYGDLDQNQN 143

Query: 124 YNDDRYDSYDEDEDSSSSSFNLEHNPVNSFHHPTARMF-GEVYGRLFGDSENLYVRPNSY 183
           YN D YDSY  +EDS SS F+LE NPV S HHPTA +  G VYGRL+GDSEN+Y  PNSY
Sbjct: 144 YNFDVYDSY--EEDSGSSLFSLEDNPVGSSHHPTAGLLGGTVYGRLYGDSENVYRHPNSY 203

Query: 184 ALTGNNSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
            L+G+NSPR RR+E +AE+SL R+S  LF  F++CLA+F
Sbjct: 204 GLSGSNSPRLRRQEIVAEKSLNRISFFLFCCFILCLAVF 240

[Close]

BLAST of Sed0019527 vs. ExPASy TrEMBL
Match: A0A6J1EJS4 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita moschata OX=3662 GN=LOC111433961 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 1.1e-81
Identity = 158/219 (72.15%), Postives = 179/219 (81.74%), Query Frame = 0


Query: 4   KFKACFDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEEPPQCPVCKSDINH 63
           KFKACFDCNICL+FA EPIVTLCGHLYCWPCIYKWL + SASL P+EPPQCPVCK+DI+H
Sbjct: 25  KFKACFDCNICLEFASEPIVTLCGHLYCWPCIYKWLHIQSASLLPDEPPQCPVCKADISH 84

Query: 64  TTMIPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDNA------YGDLYQNEN 123
           TTM+PLYGR QSA++AEVD +GLLIPPRPSAFG K LA I S+NA      YGDLYQN+N
Sbjct: 85  TTMVPLYGRDQSAKEAEVDPRGLLIPPRPSAFGNKVLASIGSNNASGEQLVYGDLYQNQN 144

Query: 124 YNDDRYDSYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGE-VYGRLFGDSENLYVRPNSY 183
            N D YDSY  +EDSSSS FN+E NPV SF HPTARMFGE VYGRLFG SENLY  PNSY
Sbjct: 145 DNFDMYDSY--EEDSSSSLFNMEDNPVGSFGHPTARMFGETVYGRLFGSSENLYAHPNSY 204

Query: 184 ALTGNNSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
           ALTG+ SP+ R    + E+SL R+S+ LF  FV+CLA+F
Sbjct: 205 ALTGSGSPQLR----MQEKSLNRISIFLFCCFVLCLAIF 237

[Close]

BLAST of Sed0019527 vs. ExPASy TrEMBL
Match: A0A0A0LUK5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G073810 PE=4 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 2.5e-81
Identity = 155/219 (70.78%), Postives = 179/219 (81.74%), Query Frame = 0


Query: 4   KFKACFDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEEPPQCPVCKSDINH 63
           KFK CFDCNICLDFA +PIVTLCGHLYCWPCIYKWL V SASLAP+EPPQCPVCK++I+H
Sbjct: 24  KFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWLHVQSASLAPDEPPQCPVCKANISH 83

Query: 64  TTMIPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDN------AYGDLYQNEN 123
           TTM+PLYGRGQSAE AEVDA+G+LIPPRPSAFG K LA IRS+N      AYGDL QN+N
Sbjct: 84  TTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKALATIRSNNASSQQFAYGDLDQNQN 143

Query: 124 YNDDRYDSYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGE-VYGRLFGDSENLYVRPNSY 183
           YN D YDSY  +EDS SS F+LE NPV S HHPTA M GE VYGR + DSEN+Y  PNSY
Sbjct: 144 YNFDVYDSY--EEDSGSSLFSLEDNPVGSSHHPTAGMLGETVYGRFYWDSENIYRHPNSY 203

Query: 184 ALTGNNSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
            L+G+NSPR RR+E +AE+SL ++   LF  F++CLA+F
Sbjct: 204 GLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF 240

[Close]

BLAST of Sed0019527 vs. ExPASy TrEMBL
Match: A0A6J1CFW3 (E3 ubiquitin-protein ligase RMA1H1-like OS=Momordica charantia OX=3673 GN=LOC111010843 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.4e-79
Identity = 155/218 (71.10%), Postives = 175/218 (80.28%), Query Frame = 0


Query: 5   FKACFDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEEPPQCPVCKSDINHT 64
           FKACFDC+ICLDFA EPIVTLCGHLYCWPCIYKWL V SASL P+EPPQCPVCK+DI+HT
Sbjct: 25  FKACFDCSICLDFASEPIVTLCGHLYCWPCIYKWLHVQSASLGPDEPPQCPVCKADISHT 84

Query: 65  TMIPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDNA------YGDLYQNENY 124
           TMIPLYG GQSAED EVDAK LLIPPRPSAFG K L  IRS+NA      Y + YQN++Y
Sbjct: 85  TMIPLYGHGQSAEDDEVDAKELLIPPRPSAFGDKALGSIRSNNASGQHLPYRNPYQNQSY 144

Query: 125 NDDRYDSYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGE-VYGRLFGDSENLYVRPNSYA 184
           N D YDSY  +EDSSSS FNLEH+P +SFHHP ARMFGE VY R+FG+SE LY  PNSY 
Sbjct: 145 NFDPYDSY--EEDSSSSLFNLEHDPADSFHHPVARMFGEMVYTRMFGNSEGLYTYPNSYP 204

Query: 185 LTGNNSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
            TG++SPR RR+E LAE+SL R+S  LF   V+ L +F
Sbjct: 205 QTGSSSPRLRRQEMLAEKSLNRISFSLFCCLVLSLIIF 240

[Close]

BLAST of Sed0019527 vs. TAIR 10
Match: AT4G27470.1 (RING membrane-anchor 3 )

HSP 1 Score: 129.0 bits (323), Expect = 4.5e-30
Identity = 82/216 (37.96%), Postives = 123/216 (56.94%), Query Frame = 0


Query: 8   CFDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEE-PPQCPVCKSDINHTTM 67
           CFDCNICLD A +P+VTLCGHL+CWPCIYKWL V  +S++ ++    CPVCKS+I  T++
Sbjct: 41  CFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNITITSL 100

Query: 68  IPLYGRGQSAEDA-----EVDAKGLLIPPRPSAFGTKDLAPIRSDNAYGDLYQNENYNDD 127
           +PLYGRG S+  +     + DA    IP RP+    ++  PI S ++     Q++  +  
Sbjct: 101 VPLYGRGMSSPSSTFGSKKQDALSTDIPRRPAPSALRN--PITSASSLNPSLQHQTLSPS 160

Query: 128 RYD-SYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGE-VYGRLFGDSENLYVRPNSYALT 187
            ++  Y     +++ S +L +  + SF +P   MFG+ VY R+FG   N   +P      
Sbjct: 161 FHNHQYSPRGFTTTESTDLANAVMMSFLYPVIGMFGDLVYTRIFGTFTNTIAQP------ 220

Query: 188 GNNSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
              S R  +R    E+SL R+S+      ++CL LF
Sbjct: 221 -YQSQRMMQR----EKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of Sed0019527 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-28
Identity = 86/212 (40.57%), Postives = 115/212 (54.25%), Query Frame = 0


Query: 9   FDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPE--EPPQCPVCKSDINHTTM 68
           FDCNICLD   EP+VTLCGHL+CWPCI+KWL V S S + E     QCPVCKS ++H+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 69  IPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDNAYGD--LYQNENYNDDRYD 128
           +PLYGRG+     E       +P RP       L    S  A  D  L Q  ++N  +  
Sbjct: 106 VPLYGRGRCTTQEEGKNS---VPKRPVG-PVYRLEMPNSPYASTDLRLSQRVHFNSPQEG 165

Query: 129 SYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGE-VYGRLFGDS-ENLYVRPNSYALTGNN 188
            Y      SS+S +      ++   P   M GE V  RLFG    + +  P++Y L G +
Sbjct: 166 YYPVSGVMSSNSLS-----YSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLAGTS 225

Query: 189 SPRSRRRETLAEQSLKRMSLCLFYFFVVCLAL 215
            PR RRR   A++SL R    +F+FF+ C+ L
Sbjct: 226 GPRMRRRIMQADKSLGR----IFFFFMCCVVL 244

[Close]

BLAST of Sed0019527 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-28
Identity = 86/212 (40.57%), Postives = 115/212 (54.25%), Query Frame = 0


Query: 9   FDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPE--EPPQCPVCKSDINHTTM 68
           FDCNICLD   EP+VTLCGHL+CWPCI+KWL V S S + E     QCPVCKS ++H+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 69  IPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDNAYGD--LYQNENYNDDRYD 128
           +PLYGRG+     E       +P RP       L    S  A  D  L Q  ++N  +  
Sbjct: 106 VPLYGRGRCTTQEEGKNS---VPKRPVG-PVYRLEMPNSPYASTDLRLSQRVHFNSPQEG 165

Query: 129 SYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGE-VYGRLFGDS-ENLYVRPNSYALTGNN 188
            Y      SS+S +      ++   P   M GE V  RLFG    + +  P++Y L G +
Sbjct: 166 YYPVSGVMSSNSLS-----YSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLAGTS 225

Query: 189 SPRSRRRETLAEQSLKRMSLCLFYFFVVCLAL 215
            PR RRR   A++SL R    +F+FF+ C+ L
Sbjct: 226 GPRMRRRIMQADKSLGR----IFFFFMCCVVL 244

[Close]

BLAST of Sed0019527 vs. TAIR 10
Match: AT4G28270.1 (RING membrane-anchor 2 )

HSP 1 Score: 110.2 bits (274), Expect = 2.2e-24
Identity = 79/214 (36.92%), Postives = 104/214 (48.60%), Query Frame = 0


Query: 9   FDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLA-------PEEPPQCPVCKSDI 68
           FDCNICLD   +P+VTLCGHL+CWPCI+KW    + S           EPP+CPVCKSD+
Sbjct: 19  FDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKSDV 78

Query: 69  NHTTMIPLYGRGQSAEDAEVDAKGLLIPPRPSAFGTKDLAPIRSDNAYGDLYQNENYNDD 128
           +  T++P+YGRGQ A  +     G  +P RP+                G +Y      D 
Sbjct: 79  SEATLVPIYGRGQKAPQS-----GSNVPSRPT----------------GPVY------DL 138

Query: 129 RYDSYDEDEDSSSSSFNLEHNPVNSFHHPTARMFGEVYGRLFGDSENLYVRPNSYALTGN 188
           R       E  S        +PV         +   VY RLFG+S       ++ A   +
Sbjct: 139 RGVGQRLGEGESQRYMYRMPDPVMGV------VCEMVYRRLFGESS------SNMAPYRD 193

Query: 189 NSPRSRRRETLAEQSLKRMSLCLFYFFVVCLALF 216
            + RSRRR   AE+SL R+ L L  F  +CL LF
Sbjct: 199 MNVRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of Sed0019527 vs. TAIR 10
Match: AT1G19310.1 (RING/U-box superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 8.2e-24
Identity = 48/86 (55.81%), Postives = 60/86 (69.77%), Query Frame = 0


Query: 9   FDCNICLDFAFEPIVTLCGHLYCWPCIYKWLRVHSASLAPEEPPQCPVCKSDINHTTMIP 68
           F+CNICLD A +PIVTLCGHL+CWPC+YKWL +HS S        CPVCK+ I    ++P
Sbjct: 21  FECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQS------KDCPVCKAVIEEDRLVP 80

Query: 69  LYGRGQSAEDAEVDA-KGLLIPPRPS 94
           LYGRG+S+ D    +  GL +P RPS
Sbjct: 81  LYGRGKSSADPRSKSIPGLEVPNRPS 100

The following BLAST results are available for this feature:

BLAST of Sed0019527 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023519102.1	2.8e-82	73.06	E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo] >XP_0235191...	[more]
XP_008442466.1	2.8e-82	72.15	PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >XP_008442474....	[more]
KAG7019622.1	1.4e-81	72.60	E3 ubiquitin-protein ligase RMA1H1, partial [Cucurbita argyrosperma subsp. argyr...	[more]
KAG6584000.1	1.4e-81	72.60	E3 ubiquitin-protein ligase RMA1H1, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_038895713.1	1.8e-81	71.69	E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida]	[more]

BLAST of Sed0019527 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6R567	1.0e-34	44.04	E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=...	[more]
Q8GUK7	6.3e-29	37.96	E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV...	[more]
O64425	1.6e-27	40.57	E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV...	[more]
P93030	3.0e-23	36.92	E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV...	[more]
Q99942	5.5e-17	47.13	E3 ubiquitin-protein ligase RNF5 OS=Homo sapiens OX=9606 GN=RNF5 PE=1 SV=1	[more]

BLAST of Sed0019527 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B6D8	1.3e-82	72.15	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo OX=3656 GN=LOC103486320 ...	[more]
A0A5A7UTV8	1.3e-82	72.15	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1EJS4	1.1e-81	72.15	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A0A0LUK5	2.5e-81	70.78	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G073810 P...	[more]
A0A6J1CFW3	1.4e-79	71.10	E3 ubiquitin-protein ligase RMA1H1-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of Sed0019527 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27470.1	4.5e-30	37.96	RING membrane-anchor 3	[more]
AT4G03510.1	1.1e-28	40.57	RING membrane-anchor 1	[more]
AT4G03510.2	1.1e-28	40.57	RING membrane-anchor 1	[more]
AT4G28270.1	2.2e-24	36.92	RING membrane-anchor 2	[more]
AT1G19310.1	8.2e-24	55.81	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 11..57 e-value: 1.0E-8 score: 45.0
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 11..58 score: 12.120453
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 2..86 e-value: 2.1E-15 score: 58.8
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 11..57 e-value: 3.9E-8 score: 33.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 111..142
None	No IPR available	PANTHER	PTHR12313:SF6	E3 UBIQUITIN-PROTEIN LIGASE RMA3	coord: 5..215
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 9..59 e-value: 1.89523E-24 score: 89.2855
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 7..70
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 5..215
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 26..35

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0019527.1	Sed0019527.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071712	ER-associated misfolded protein catabolic process
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0044390	ubiquitin-like protein conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity