Sed0019254 (gene) Chayote v1

Overview
NameSed0019254
Typegene
OrganismSechium edule (Chayote v1)
DescriptionE3 ubiquitin-protein ligase RMA1H1-like
LocationLG10: 1847380 .. 1851002 (+)
RNA-Seq ExpressionSed0019254
SyntenySed0019254
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTAAAGAAAACGGAGGTTGGTCAATGAAACCCCATTCCCATTGGTCGGTTTCTTTCCGTCTGTTTCCTCCGTTTCCCATTCCGTCATTTTGTTCTTCATCATTAACTTCGCCTCTTTTAGTGGGAAGCTCTGAACAACGAATCGCCGGTTCTATCTCTTCCTGTCTCAGGTGAATCACTGAATCTGAAATTTTCATGATTTGTTTTCGCTTCTCAACTTGTATTTTCATGTTGATTTGTTGATTAATCCATTTTCGTTTTAATTTCCATGTCTTTTTTTTTTTGGTGTTAGTTCTCAATTTCTTGATCTTTGATTCTGGATATGTTTTTGTGTGGCTCTATTTCTTTCTTCTGGAGTAACAGTGGAAGCTTCAACGTTTTCATGTGTTTTCCTCAGTGTGTTTCGGTTTAATTTGAGGATTGGAAATGTGGTATTTGGTTGTTCTTATGATAATTCTATTTATGTCAGAACAATCTTTGATTTTAAGTGAAGGTTTAGCTCTTCTAGGAACTTTTTTGGGTGAGTATTTGGATGAGGAGTTATTTTGAGAAGTTAACCATGAGTAGTTAGAACATTGGGGTTTTTATAATAACTCATTTTTTATGGAGTAAAAAACTCGTTCAGATGTGGTTTTATTTCGTAGAAGAGTCAAAGACAATAGTTTGTTTGGACCTCAATTTTAAATGAGGAGTTAAAAATTAATGATTAGAAATGAGTGGTTAAAAATAAGTTATTTTAAGTAGTTAGAAAATTGGGGTTTTCATAATAACTCGTTTTTTATGCAGTAAAAAAACTCATTTGAATGTTGTTTTATTTTGTAGAAGAGTTAACAATAGTTTCTTTAAATATGAATTTGAAATGATGAGTTATTTTGAGAAGTTTAAAATTAATGATTAGAAATGGGTGGGTAAAATGAGTTATTTTGAATAGTTGGAAATGAATGATTAAAAATGAGAAGTTATTTTGAGTAGCTAGAAATGAATGATTGAAAATGAGTGGTTAGAAATAAGTATTTATTTTGAGTAGTTAGAAATGAAGATTTATTTTGAGTAGTTACAAAAAAACTCGTTTAGATGTAGTTTTTTTTAGGATTAGTTAGTAAATGCTCAATCTAGGTTGTGATTTTTATAAGTTCTTTAAAATGTAAGGCACATAGGTTGTTCCATATCTTTACCACAAGAGTTCTCCCATTTTATGCTTGAAAAGGAGCAGGGTTGGATCATCACTACTCTGGTTGATGATTTTCATCATTATTGATTGCTTTGTCAGGCAATCAACCATTTTCATCAGTCGTTCGCGGGCCGAAAATAATCTGAGCAACACACACTCTTACACTGGAGTTATTTAGCCTTTGGCTTTAACGGTTTGGTTTTGTTTGTGAACATGATTTGTTCTTACATGTCTAGTATTAGCTATAAACTAGGACATCAGAGTATTTTTCCATTTGGAAGAAAAGTTTTGCCCTCTAAATTCTGTTCCTTTTTCTTGTGAATAGATTTTGAACTCCTCCACCTGTTTACTATCTCCATGTTTATTTTATGTGGCATTTATGACTCTATCTCCTTCCATTTGGGCTATTTAAGGGAATCAAGAAGAGGATTAGTCTTATAGAGGGATCATTTGAAATTTTGTTTATGATTTTCACTTCTGGATGGAATCCTAAACATTGAACAGGGGGGTGAAAGGGAGAAGTGGTGTGTACATTTTCCTGGTGGTTCTGAGAAATACTGATATTGTTTCTGAAGTTTATATCTGATAAATGATAATGTTTCTGCTTTTGCACAACATGAATTTGTGTAAACAGGTCAAATTGTTTTCCATGTTATGTTTGTTATATTCATAGGCTGGCAGCTGGCTTGATCATTGAACTAGAACCATGTGTCAAAAACAATAGGTTGGTCTCTTGTGATGATGAAATGGATCACAAGCTAGAAAAGCTTTTTATATCTAGAACTAGAACATAAGCTATAAAATTGTGACTTCCAAGCTTTAGAAGTTTGTATTTTACATTTACTTAAAAACAACATTTGAACTAAAGGGTAATGGGCTACATAAATGGACCACTGATGAGGACCATGAGCCAGGTTTACATTTTTCTTTTTGGAGTTAACTATGACTTCAATATTTGGGCCCTCCCTTCTGTATTTATTTGTATGGCCTATAAGTAAATAATGTAGTCCTTGTTTTAGTCACTGGACCTACTTGTATTATACACCCATGGTTAGCTCCAAGAGGTGACTTGTTGACTGCTTTAATTACTAATGTATTAAAATAGCTGTTCATAACCGATTTCGCCAACTGATTGAAGTTCTACAAAGTTTGAGAACAAAACACAAATCAATTTAAACCTCTTTCAATTTGACGCCAATCGGTAGATAACCATCATAGATTAAATTCATTTCCTCTAAGTTCCCAACCTTTTGACCCCTAGATTGTATGGCTAGATATGACTACCACATGATGGTTTGGTTGCTATTTTATTGTTATTAATATTACCCACCTTAACTCTAACCTTAGTAATAGTTTGCTTCCAAGTCTGTTTCATTATGTGTTTGGTATTTGAGCATTTAAACTCTTGTAGGATGTAGGATGAGTTGATTGGGCATTCATGATCTAACTACTGAAATAAAGTTGATTTCAAAAAAAAAAAAAAATCTACTGAAATAAAGTCGGCCACATGGTTTGTTCCTTCCAATTGGGTTTTATCTTATTTGTCTCACCAACCGTCTGTTTTGGTAGTCTTTACATTATTTATTGTTTGCACCTCTGGCTTTCACCTTAGCTTCTAATTCTGTTTTTAATTTCCAAAATCATTTCAGACTAAAAAATTTGCTGTCTAATTCATTTTTGTGTGTGTGTTTTTTTTCCAGGAAATAACTGGCTTAACCAATCCATGGCCTTTGAGCAGTACTTTATGCCAGAATTGAAACACGCCTCTGGTACTCCGGGAACTGATCGAGAGACCTACAAAGGTTGCTTTGATTGCAACATCTGCTTTGACTTTGCCCATGAGCCAGTAGTTACTCTCTGCGGCCACTTATACTGCTGGCCTTGCATCTATAAATGGCTTCACGTCCAGAGCGCCTCGCTTGCCATTGACGAGCACCCGCAATGCCCAGTTTGCAAGGCTGATATTTCCCACACCACAATGGTTCCTCTTTATGGTCGTGGCCAAACTGCCGAGGAAGTCGAGCCTGAAGCCAATTCAATGCATCAAGACATCGATATACCTCCTAGACCTTCAGCTTGTGGTAACCAGATACTTGCTTCTCCCAGCACACACCAACTGCAACTTCCATATCGAAATCCTTACCAACGACCGAACCACAACCCTCTTCTCTTTGGCAGCTATGAAGAAGATTCAGCAGCTCCTTTGCTAAACCTTGCAAGAACATCATTCAGTGGCTTTCATCATCCGGTGGTAGGAATGATTGGCGATTTCGTGTATGCAAGGGTGTTTGGGAACTCGGACAACTTGTACTCATACCGAAACTCTTACCATCTAACAGGGAGCAGGAGGAACAGGTTGAGAAGACAAGAAATGCAGGTGGACAAATCATTGAACAGAATCTCCATTTTCCTCTTGTGCTGTGTGATTCTGTGCATTCTTGTCTTCTGA

mRNA sequence

ATGGTTAAAGAAAACGGAGGTTGGTCAATGAAACCCCATTCCCATTGGTCGGTTTCTTTCCGTCTGTTTCCTCCGTTTCCCATTCCGTCATTTTGTTCTTCATCATTAACTTCGCCTCTTTTAGCACATAGGTTGTTCCATATCTTTACCACAAGAGTTCTCCCATTTTATCAGTACTTTATGCCAGAATTGAAACACGCCTCTGGTACTCCGGGAACTGATCGAGAGACCTACAAAGGTTGCTTTGATTGCAACATCTGCTTTGACTTTGCCCATGAGCCAGTAGTTACTCTCTGCGGCCACTTATACTGCTGGCCTTGCATCTATAAATGGCTTCACGTCCAGAGCGCCTCGCTTGCCATTGACGAGCACCCGCAATGCCCAGTTTGCAAGGCTGATATTTCCCACACCACAATGGTTCCTCTTTATGGTCGTGGCCAAACTGCCGAGGAAGTCGAGCCTGAAGCCAATTCAATGCATCAAGACATCGATATACCTCCTAGACCTTCAGCTTGTGGTAACCAGATACTTGCTTCTCCCAGCACACACCAACTGCAACTTCCATATCGAAATCCTTACCAACGACCGAACCACAACCCTCTTCTCTTTGGCAGCTATGAAGAAGATTCAGCAGCTCCTTTGCTAAACCTTGCAAGAACATCATTCAGTGGCTTTCATCATCCGGTGGTAGGAATGATTGGCGATTTCGTGTATGCAAGGGTGTTTGGGAACTCGGACAACTTGTACTCATACCGAAACTCTTACCATCTAACAGGGAGCAGGAGGAACAGGTTGAGAAGACAAGAAATGCAGGTGGACAAATCATTGAACAGAATCTCCATTTTCCTCTTGTGCTGTGTGATTCTGTGCATTCTTGTCTTCTGA

Coding sequence (CDS)

ATGGTTAAAGAAAACGGAGGTTGGTCAATGAAACCCCATTCCCATTGGTCGGTTTCTTTCCGTCTGTTTCCTCCGTTTCCCATTCCGTCATTTTGTTCTTCATCATTAACTTCGCCTCTTTTAGCACATAGGTTGTTCCATATCTTTACCACAAGAGTTCTCCCATTTTATCAGTACTTTATGCCAGAATTGAAACACGCCTCTGGTACTCCGGGAACTGATCGAGAGACCTACAAAGGTTGCTTTGATTGCAACATCTGCTTTGACTTTGCCCATGAGCCAGTAGTTACTCTCTGCGGCCACTTATACTGCTGGCCTTGCATCTATAAATGGCTTCACGTCCAGAGCGCCTCGCTTGCCATTGACGAGCACCCGCAATGCCCAGTTTGCAAGGCTGATATTTCCCACACCACAATGGTTCCTCTTTATGGTCGTGGCCAAACTGCCGAGGAAGTCGAGCCTGAAGCCAATTCAATGCATCAAGACATCGATATACCTCCTAGACCTTCAGCTTGTGGTAACCAGATACTTGCTTCTCCCAGCACACACCAACTGCAACTTCCATATCGAAATCCTTACCAACGACCGAACCACAACCCTCTTCTCTTTGGCAGCTATGAAGAAGATTCAGCAGCTCCTTTGCTAAACCTTGCAAGAACATCATTCAGTGGCTTTCATCATCCGGTGGTAGGAATGATTGGCGATTTCGTGTATGCAAGGGTGTTTGGGAACTCGGACAACTTGTACTCATACCGAAACTCTTACCATCTAACAGGGAGCAGGAGGAACAGGTTGAGAAGACAAGAAATGCAGGTGGACAAATCATTGAACAGAATCTCCATTTTCCTCTTGTGCTGTGTGATTCTGTGCATTCTTGTCTTCTGA

Protein sequence

MVKENGGWSMKPHSHWSVSFRLFPPFPIPSFCSSSLTSPLLAHRLFHIFTTRVLPFYQYFMPELKHASGTPGTDRETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEANSMHQDIDIPPRPSACGNQILASPSTHQLQLPYRNPYQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMIGDFVYARVFGNSDNLYSYRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILVF
Homology
BLAST of Sed0019254 vs. NCBI nr
Match: XP_038881993.1 (E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881994.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881995.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881996.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881997.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881998.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida])

HSP 1 Score: 458.4 bits (1178), Expect = 4.7e-125
Identity = 213/241 (88.38%), Postives = 224/241 (92.95%), Query Frame = 0

Query: 54  LPFYQYFMPELKHASGTPGTDRETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLH 113
           + F QYF    KH SGT GTDRE YKGCFDCNIC DFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 114 VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEANSMHQDIDIPPRPSACG 173
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEA +MH DI+IPPRPSACG
Sbjct: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACG 120

Query: 174 NQILASPSTHQLQLPYRNPYQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMI 233
           NQ+LA+PS HQ QLPYRNPYQ+PNH+PLLFG+YEEDSAAPLLNLARTSFSGFHHP+VGMI
Sbjct: 121 NQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMI 180

Query: 234 GDFVYARVFGNSDNLYSYRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILV 293
           GDFV+ARVFGNSDNLYSYRNSY LTGS RNRLRRQEMQVDKSLNRISIFL CCVILC+LV
Sbjct: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 240

Query: 294 F 295
           F
Sbjct: 241 F 241

BLAST of Sed0019254 vs. NCBI nr
Match: XP_004134706.1 (E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus] >XP_031743482.1 E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus] >XP_031743483.1 E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus])

HSP 1 Score: 447.2 bits (1149), Expect = 1.1e-121
Identity = 211/246 (85.77%), Postives = 222/246 (90.24%), Query Frame = 0

Query: 49  FTTRVLPFYQYFMPELKHASGTPGTDRETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCI 108
           F  + + F QYF    KH S   GT+RE YKGCFDCNIC DFAHEPVVTLCGHLYCWPCI
Sbjct: 15  FLDKSMAFEQYFAQGWKHVS---GTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCI 74

Query: 109 YKWLHVQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEANSMHQDIDIPPR 168
           YKWLHVQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPE  +MH DI+IPPR
Sbjct: 75  YKWLHVQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPR 134

Query: 169 PSACGNQILASPSTHQLQLPYRNPYQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHP 228
           PSACGNQILA+PSTHQ QLPYRNPYQRPNH+PLLF +YEEDSA+PLLNLARTSFS FHHP
Sbjct: 135 PSACGNQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHP 194

Query: 229 VVGMIGDFVYARVFGNSDNLYSYRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVI 288
           VVGMIGDFV+ARVFGNSDNLYSYRNSY LTGS RNRLRRQEMQVDKSLNRISIFL CCVI
Sbjct: 195 VVGMIGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVI 254

Query: 289 LCILVF 295
           LC+LVF
Sbjct: 255 LCVLVF 257

BLAST of Sed0019254 vs. NCBI nr
Match: XP_004134707.1 (E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743484.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743485.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743486.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743487.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743488.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >KGN49224.1 hypothetical protein Csa_003905 [Cucumis sativus])

HSP 1 Score: 445.7 bits (1145), Expect = 3.2e-121
Identity = 210/241 (87.14%), Postives = 220/241 (91.29%), Query Frame = 0

Query: 54  LPFYQYFMPELKHASGTPGTDRETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLH 113
           + F QYF    KH S   GT+RE YKGCFDCNIC DFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFEQYFAQGWKHVS---GTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 114 VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEANSMHQDIDIPPRPSACG 173
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPE  +MH DI+IPPRPSACG
Sbjct: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACG 120

Query: 174 NQILASPSTHQLQLPYRNPYQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMI 233
           NQILA+PSTHQ QLPYRNPYQRPNH+PLLF +YEEDSA+PLLNLARTSFS FHHPVVGMI
Sbjct: 121 NQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMI 180

Query: 234 GDFVYARVFGNSDNLYSYRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILV 293
           GDFV+ARVFGNSDNLYSYRNSY LTGS RNRLRRQEMQVDKSLNRISIFL CCVILC+LV
Sbjct: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 238

Query: 294 F 295
           F
Sbjct: 241 F 238

BLAST of Sed0019254 vs. NCBI nr
Match: XP_023003183.1 (E3 ubiquitin-protein ligase RMA1H1-like isoform X1 [Cucurbita maxima] >XP_023003184.1 E3 ubiquitin-protein ligase RMA1H1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 441.4 bits (1134), Expect = 6.0e-120
Identity = 207/242 (85.54%), Postives = 220/242 (90.91%), Query Frame = 0

Query: 54  LPFYQYFMPELKHASGTPGTDRETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLH 113
           + + Q F    KH SGT GTDRETYKGCFDCNIC DFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAYEQCFAQGWKHVSGTGGTDRETYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 114 VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEANSMHQD-IDIPPRPSAC 173
           V+S SLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEV PEA ++HQD +DIPPRPSAC
Sbjct: 61  VKSVSLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVGPEAQALHQDNVDIPPRPSAC 120

Query: 174 GNQILASPSTHQLQLPYRNPYQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGM 233
           GNQILA+ STHQ QL YRNPYQRPNH+PLLFG+YEEDS AP LNLARTSFSGFHHPVVGM
Sbjct: 121 GNQILATLSTHQQQLRYRNPYQRPNHDPLLFGNYEEDSPAPSLNLARTSFSGFHHPVVGM 180

Query: 234 IGDFVYARVFGNSDNLYSYRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCIL 293
           IGDF+YARVFGNSDNLYSY+NSYHLTGSRRNRLRRQEMQ +KSLNRISIFL CCVILC+L
Sbjct: 181 IGDFMYARVFGNSDNLYSYQNSYHLTGSRRNRLRRQEMQAEKSLNRISIFLFCCVILCVL 240

Query: 294 VF 295
           VF
Sbjct: 241 VF 242

BLAST of Sed0019254 vs. NCBI nr
Match: XP_008439861.1 (PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >KAA0052689.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo var. makuwa] >TYK13135.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo var. makuwa])

HSP 1 Score: 440.7 bits (1132), Expect = 1.0e-119
Identity = 209/241 (86.72%), Postives = 219/241 (90.87%), Query Frame = 0

Query: 54  LPFYQYFMPELKHASGTPGTDRETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLH 113
           + F QYF    KH S   GTDRE YKGCFDCNIC DFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFDQYFAQGWKHVS---GTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 114 VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEANSMHQDIDIPPRPSACG 173
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEVE EA +MH+DI+IPPRPSACG
Sbjct: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVELEAKAMHRDINIPPRPSACG 120

Query: 174 NQILASPSTHQLQLPYRNPYQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMI 233
           NQILA+PSTHQ QLPYRNPYQRPNH+PLLF +YEE SA+PLLNLARTSFS FHHPVVGMI
Sbjct: 121 NQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEHSASPLLNLARTSFSSFHHPVVGMI 180

Query: 234 GDFVYARVFGNSDNLYSYRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILV 293
           GDFV+ARVFGNSDNLYSYRNSY LTGS RNRLRRQEMQVDKSLNRISIFL CCVILC+LV
Sbjct: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 238

Query: 294 F 295
           F
Sbjct: 241 F 238

BLAST of Sed0019254 vs. ExPASy Swiss-Prot
Match: Q6R567 (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.3e-45
Identity = 101/226 (44.69%), Postives = 139/226 (61.50%), Query Frame = 0

Query: 76  ETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLHVQSASL--AIDEHPQCPVCKAD 135
           E   G FDCNIC D  HEPV+TLCGHLYCWPCIYKW++ QS S   +  + PQCPVCKA+
Sbjct: 33  ENISGGFDCNICLDCVHEPVITLCGHLYCWPCIYKWIYFQSVSSENSDQQQPQCPVCKAE 92

Query: 136 ISHTTMVPLYGR-GQTAEEVEPEANSMHQDI-DIPPRPSACGNQILASPSTHQLQLPYRN 195
           +S  T++PLYGR GQ+ +  E +A ++   I   PP P   G+ +L +  ++  QL  R 
Sbjct: 93  VSEKTLIPLYGRGGQSTKPSEGKAPNLGIVIPQRPPSPRCGGHFLLPTTDSNPSQLLQRR 152

Query: 196 PYQRPN--HNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMIGDFVYARVFGNSD-NL 255
            YQ+ +    P   GSY    ++P+L+    + +   H    MIG+  YAR+FGNS   +
Sbjct: 153 GYQQQSQTRQPAYQGSY---MSSPMLSPGGATANMLQH---SMIGEVAYARIFGNSSTTM 212

Query: 256 YSYRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILVF 295
           Y+Y NSY+L  S   R+RRQ  Q D+SL RI  FL CC + C+++F
Sbjct: 213 YTYPNSYNLAISSSPRMRRQLSQADRSLGRICFFLFCCFVTCLILF 252

BLAST of Sed0019254 vs. ExPASy Swiss-Prot
Match: Q8GUK7 (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.9e-44
Identity = 98/230 (42.61%), Postives = 138/230 (60.00%), Query Frame = 0

Query: 71  PGTDRETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHP-QCPV 130
           P   +    GCFDCNIC D AH+PVVTLCGHL+CWPCIYKWLHVQ +S+++D+H   CPV
Sbjct: 31  PTAGQANESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPV 90

Query: 131 CKADISHTTMVPLYGRGQTAEEV---EPEANSMHQDIDIPPRPSACGNQILASPSTH-QL 190
           CK++I+ T++VPLYGRG ++        + +++  DI   P PSA  N I ++ S +  L
Sbjct: 91  CKSNITITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRPAPSALRNPITSASSLNPSL 150

Query: 191 QLPYRNP-YQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMIGDFVYARVFGN 250
           Q    +P +    ++P  F + E        +LA      F +PV+GM GD VY R+FG 
Sbjct: 151 QHQTLSPSFHNHQYSPRGFTTTES------TDLANAVMMSFLYPVIGMFGDLVYTRIFGT 210

Query: 251 SDNLYSYRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILVF 295
             N  +           +    ++ MQ +KSLNR+SIF LCC+ILC+L+F
Sbjct: 211 FTNTIA-----------QPYQSQRMMQREKSLNRVSIFFLCCIILCLLLF 243

BLAST of Sed0019254 vs. ExPASy Swiss-Prot
Match: O64425 (E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.2e-38
Identity = 94/224 (41.96%), Postives = 133/224 (59.38%), Query Frame = 0

Query: 82  FDCNICFDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLA--IDEHPQCPVCKADISHTTM 141
           FDCNIC D   EPVVTLCGHL+CWPCI+KWL VQS S +     H QCPVCK+ +SH+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 142 VPLYGRGQTAEEVEPEANSMHQDIDIPPRPSACGNQILASPSTHQLQLPYRNPY------ 201
           VPLYGRG+   + E + NS      +P RP             ++L++P  +PY      
Sbjct: 106 VPLYGRGRCTTQEEGK-NS------VPKRPVG---------PVYRLEMP-NSPYASTDLR 165

Query: 202 --QRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMIGDFVYARVFGNS-DNLYS 261
             QR + N    G Y     + +++    S+S    PV+ M+G+ V  R+FG    + ++
Sbjct: 166 LSQRVHFNSPQEGYY---PVSGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFA 225

Query: 262 YRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILVF 295
           Y ++Y+L G+   R+RR+ MQ DKSL RI  F +CCV+LC+L+F
Sbjct: 226 YPDTYNLAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

BLAST of Sed0019254 vs. ExPASy Swiss-Prot
Match: P93030 (E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 9.6e-28
Identity = 77/223 (34.53%), Postives = 116/223 (52.02%), Query Frame = 0

Query: 80  GCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLHVQSAS-LAIDEH------PQCPVCKA 139
           G FDCNIC D   +PVVTLCGHL+CWPCI+KW +  + S   +D++      P+CPVCK+
Sbjct: 17  GDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKS 76

Query: 140 DISHTTMVPLYGRGQTAEEVEPEANSMHQDIDIPPRPSACGNQILASPSTHQLQLPYRNP 199
           D+S  T+VP+YGRGQ A    P++ S     ++P RP+     +             R  
Sbjct: 77  DVSEATLVPIYGRGQKA----PQSGS-----NVPSRPTGPVYDLRGVGQRLGEGESQRYM 136

Query: 200 YQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMIGDFVYARVFG-NSDNLYSY 259
           Y+ P+                              PV+G++ + VY R+FG +S N+  Y
Sbjct: 137 YRMPD------------------------------PVMGVVCEMVYRRLFGESSSNMAPY 193

Query: 260 RNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILVF 295
           R+          R RR+ MQ ++SL+R+ +FLLC + +C+ +F
Sbjct: 197 RD-------MNVRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

BLAST of Sed0019254 vs. ExPASy Swiss-Prot
Match: Q99942 (E3 ubiquitin-protein ligase RNF5 OS=Homo sapiens OX=9606 GN=RNF5 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.2e-17
Identity = 43/99 (43.43%), Postives = 57/99 (57.58%), Query Frame = 0

Query: 71  PGTDRETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHPQCPVC 130
           P  +R      F+CNIC + A E VV++CGHLYCWPC+++WL  +       E  +CPVC
Sbjct: 14  PNRERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETR------PERQECPVC 73

Query: 131 KADISHTTMVPLYGRGQTAEEVEPEANSMHQDIDIPPRP 170
           KA IS   +VPLYGRG    + +P        +  PPRP
Sbjct: 74  KAGISREKVVPLYGRGSQKPQ-DPR-------LKTPPRP 98

BLAST of Sed0019254 vs. ExPASy TrEMBL
Match: A0A0A0KK60 (Rnf5 OS=Cucumis sativus OX=3659 GN=Csa_6G517320 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.5e-121
Identity = 210/241 (87.14%), Postives = 220/241 (91.29%), Query Frame = 0

Query: 54  LPFYQYFMPELKHASGTPGTDRETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLH 113
           + F QYF    KH S   GT+RE YKGCFDCNIC DFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFEQYFAQGWKHVS---GTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 114 VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEANSMHQDIDIPPRPSACG 173
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPE  +MH DI+IPPRPSACG
Sbjct: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACG 120

Query: 174 NQILASPSTHQLQLPYRNPYQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMI 233
           NQILA+PSTHQ QLPYRNPYQRPNH+PLLF +YEEDSA+PLLNLARTSFS FHHPVVGMI
Sbjct: 121 NQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMI 180

Query: 234 GDFVYARVFGNSDNLYSYRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILV 293
           GDFV+ARVFGNSDNLYSYRNSY LTGS RNRLRRQEMQVDKSLNRISIFL CCVILC+LV
Sbjct: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 238

Query: 294 F 295
           F
Sbjct: 241 F 238

BLAST of Sed0019254 vs. ExPASy TrEMBL
Match: A0A6J1KVR4 (E3 ubiquitin-protein ligase RMA1H1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496874 PE=4 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.9e-120
Identity = 207/242 (85.54%), Postives = 220/242 (90.91%), Query Frame = 0

Query: 54  LPFYQYFMPELKHASGTPGTDRETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLH 113
           + + Q F    KH SGT GTDRETYKGCFDCNIC DFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAYEQCFAQGWKHVSGTGGTDRETYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 114 VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEANSMHQD-IDIPPRPSAC 173
           V+S SLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEV PEA ++HQD +DIPPRPSAC
Sbjct: 61  VKSVSLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVGPEAQALHQDNVDIPPRPSAC 120

Query: 174 GNQILASPSTHQLQLPYRNPYQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGM 233
           GNQILA+ STHQ QL YRNPYQRPNH+PLLFG+YEEDS AP LNLARTSFSGFHHPVVGM
Sbjct: 121 GNQILATLSTHQQQLRYRNPYQRPNHDPLLFGNYEEDSPAPSLNLARTSFSGFHHPVVGM 180

Query: 234 IGDFVYARVFGNSDNLYSYRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCIL 293
           IGDF+YARVFGNSDNLYSY+NSYHLTGSRRNRLRRQEMQ +KSLNRISIFL CCVILC+L
Sbjct: 181 IGDFMYARVFGNSDNLYSYQNSYHLTGSRRNRLRRQEMQAEKSLNRISIFLFCCVILCVL 240

Query: 294 VF 295
           VF
Sbjct: 241 VF 242

BLAST of Sed0019254 vs. ExPASy TrEMBL
Match: A0A5A7UGP7 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007450 PE=4 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 5.0e-120
Identity = 209/241 (86.72%), Postives = 219/241 (90.87%), Query Frame = 0

Query: 54  LPFYQYFMPELKHASGTPGTDRETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLH 113
           + F QYF    KH S   GTDRE YKGCFDCNIC DFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFDQYFAQGWKHVS---GTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 114 VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEANSMHQDIDIPPRPSACG 173
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEVE EA +MH+DI+IPPRPSACG
Sbjct: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVELEAKAMHRDINIPPRPSACG 120

Query: 174 NQILASPSTHQLQLPYRNPYQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMI 233
           NQILA+PSTHQ QLPYRNPYQRPNH+PLLF +YEE SA+PLLNLARTSFS FHHPVVGMI
Sbjct: 121 NQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEHSASPLLNLARTSFSSFHHPVVGMI 180

Query: 234 GDFVYARVFGNSDNLYSYRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILV 293
           GDFV+ARVFGNSDNLYSYRNSY LTGS RNRLRRQEMQVDKSLNRISIFL CCVILC+LV
Sbjct: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 238

Query: 294 F 295
           F
Sbjct: 241 F 238

BLAST of Sed0019254 vs. ExPASy TrEMBL
Match: A0A1S3AZB8 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo OX=3656 GN=LOC103484523 PE=4 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 5.0e-120
Identity = 209/241 (86.72%), Postives = 219/241 (90.87%), Query Frame = 0

Query: 54  LPFYQYFMPELKHASGTPGTDRETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLH 113
           + F QYF    KH S   GTDRE YKGCFDCNIC DFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFDQYFAQGWKHVS---GTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 114 VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEANSMHQDIDIPPRPSACG 173
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEVE EA +MH+DI+IPPRPSACG
Sbjct: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVELEAKAMHRDINIPPRPSACG 120

Query: 174 NQILASPSTHQLQLPYRNPYQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMI 233
           NQILA+PSTHQ QLPYRNPYQRPNH+PLLF +YEE SA+PLLNLARTSFS FHHPVVGMI
Sbjct: 121 NQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEHSASPLLNLARTSFSSFHHPVVGMI 180

Query: 234 GDFVYARVFGNSDNLYSYRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILV 293
           GDFV+ARVFGNSDNLYSYRNSY LTGS RNRLRRQEMQVDKSLNRISIFL CCVILC+LV
Sbjct: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 238

Query: 294 F 295
           F
Sbjct: 241 F 238

BLAST of Sed0019254 vs. ExPASy TrEMBL
Match: A0A6J1EI23 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita moschata OX=3662 GN=LOC111433592 PE=4 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 5.1e-117
Identity = 203/242 (83.88%), Postives = 215/242 (88.84%), Query Frame = 0

Query: 54  LPFYQYFMPELKHASGTPGTDRETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLH 113
           + + Q F    KH SGT GTDRET KGCFDCNIC DFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAYEQCFAQGWKHVSGTGGTDRETNKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 114 VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEANSMHQD-IDIPPRPSAC 173
           V+S SLAIDE PQCPVCKADISHTTMVPLYGRGQ AEEV PEA +MHQD +DIPPRPSAC
Sbjct: 61  VKSVSLAIDEQPQCPVCKADISHTTMVPLYGRGQMAEEVRPEAKAMHQDNVDIPPRPSAC 120

Query: 174 GNQILASPSTHQLQLPYRNPYQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGM 233
           GNQILA+ STHQ QL YRNPYQRPNH+PLLFG+YEED  AP LNL RTS SGFHHPVVGM
Sbjct: 121 GNQILATLSTHQQQLRYRNPYQRPNHDPLLFGNYEEDLPAPSLNLGRTSLSGFHHPVVGM 180

Query: 234 IGDFVYARVFGNSDNLYSYRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCIL 293
           IGDF+YARVFGNSDNLYSY+NSYHLTGSRRNRLRRQEMQ +KSLNRISIFL CCVILC+L
Sbjct: 181 IGDFMYARVFGNSDNLYSYQNSYHLTGSRRNRLRRQEMQAEKSLNRISIFLFCCVILCVL 240

Query: 294 VF 295
           VF
Sbjct: 241 VF 242

BLAST of Sed0019254 vs. TAIR 10
Match: AT4G27470.1 (RING membrane-anchor 3 )

HSP 1 Score: 181.0 bits (458), Expect = 1.4e-45
Identity = 98/230 (42.61%), Postives = 138/230 (60.00%), Query Frame = 0

Query: 71  PGTDRETYKGCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHP-QCPV 130
           P   +    GCFDCNIC D AH+PVVTLCGHL+CWPCIYKWLHVQ +S+++D+H   CPV
Sbjct: 31  PTAGQANESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPV 90

Query: 131 CKADISHTTMVPLYGRGQTAEEV---EPEANSMHQDIDIPPRPSACGNQILASPSTH-QL 190
           CK++I+ T++VPLYGRG ++        + +++  DI   P PSA  N I ++ S +  L
Sbjct: 91  CKSNITITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRPAPSALRNPITSASSLNPSL 150

Query: 191 QLPYRNP-YQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMIGDFVYARVFGN 250
           Q    +P +    ++P  F + E        +LA      F +PV+GM GD VY R+FG 
Sbjct: 151 QHQTLSPSFHNHQYSPRGFTTTES------TDLANAVMMSFLYPVIGMFGDLVYTRIFGT 210

Query: 251 SDNLYSYRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILVF 295
             N  +           +    ++ MQ +KSLNR+SIF LCC+ILC+L+F
Sbjct: 211 FTNTIA-----------QPYQSQRMMQREKSLNRVSIFFLCCIILCLLLF 243

BLAST of Sed0019254 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 161.8 bits (408), Expect = 8.5e-40
Identity = 94/224 (41.96%), Postives = 133/224 (59.38%), Query Frame = 0

Query: 82  FDCNICFDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLA--IDEHPQCPVCKADISHTTM 141
           FDCNIC D   EPVVTLCGHL+CWPCI+KWL VQS S +     H QCPVCK+ +SH+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 142 VPLYGRGQTAEEVEPEANSMHQDIDIPPRPSACGNQILASPSTHQLQLPYRNPY------ 201
           VPLYGRG+   + E + NS      +P RP             ++L++P  +PY      
Sbjct: 106 VPLYGRGRCTTQEEGK-NS------VPKRPVG---------PVYRLEMP-NSPYASTDLR 165

Query: 202 --QRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMIGDFVYARVFGNS-DNLYS 261
             QR + N    G Y     + +++    S+S    PV+ M+G+ V  R+FG    + ++
Sbjct: 166 LSQRVHFNSPQEGYY---PVSGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFA 225

Query: 262 YRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILVF 295
           Y ++Y+L G+   R+RR+ MQ DKSL RI  F +CCV+LC+L+F
Sbjct: 226 YPDTYNLAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

BLAST of Sed0019254 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 161.8 bits (408), Expect = 8.5e-40
Identity = 94/224 (41.96%), Postives = 133/224 (59.38%), Query Frame = 0

Query: 82  FDCNICFDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLA--IDEHPQCPVCKADISHTTM 141
           FDCNIC D   EPVVTLCGHL+CWPCI+KWL VQS S +     H QCPVCK+ +SH+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 142 VPLYGRGQTAEEVEPEANSMHQDIDIPPRPSACGNQILASPSTHQLQLPYRNPY------ 201
           VPLYGRG+   + E + NS      +P RP             ++L++P  +PY      
Sbjct: 106 VPLYGRGRCTTQEEGK-NS------VPKRPVG---------PVYRLEMP-NSPYASTDLR 165

Query: 202 --QRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMIGDFVYARVFGNS-DNLYS 261
             QR + N    G Y     + +++    S+S    PV+ M+G+ V  R+FG    + ++
Sbjct: 166 LSQRVHFNSPQEGYY---PVSGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFA 225

Query: 262 YRNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILVF 295
           Y ++Y+L G+   R+RR+ MQ DKSL RI  F +CCV+LC+L+F
Sbjct: 226 YPDTYNLAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

BLAST of Sed0019254 vs. TAIR 10
Match: AT4G28270.1 (RING membrane-anchor 2 )

HSP 1 Score: 125.6 bits (314), Expect = 6.8e-29
Identity = 77/223 (34.53%), Postives = 116/223 (52.02%), Query Frame = 0

Query: 80  GCFDCNICFDFAHEPVVTLCGHLYCWPCIYKWLHVQSAS-LAIDEH------PQCPVCKA 139
           G FDCNIC D   +PVVTLCGHL+CWPCI+KW +  + S   +D++      P+CPVCK+
Sbjct: 17  GDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKS 76

Query: 140 DISHTTMVPLYGRGQTAEEVEPEANSMHQDIDIPPRPSACGNQILASPSTHQLQLPYRNP 199
           D+S  T+VP+YGRGQ A    P++ S     ++P RP+     +             R  
Sbjct: 77  DVSEATLVPIYGRGQKA----PQSGS-----NVPSRPTGPVYDLRGVGQRLGEGESQRYM 136

Query: 200 YQRPNHNPLLFGSYEEDSAAPLLNLARTSFSGFHHPVVGMIGDFVYARVFG-NSDNLYSY 259
           Y+ P+                              PV+G++ + VY R+FG +S N+  Y
Sbjct: 137 YRMPD------------------------------PVMGVVCEMVYRRLFGESSSNMAPY 193

Query: 260 RNSYHLTGSRRNRLRRQEMQVDKSLNRISIFLLCCVILCILVF 295
           R+          R RR+ MQ ++SL+R+ +FLLC + +C+ +F
Sbjct: 197 RD-------MNVRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

BLAST of Sed0019254 vs. TAIR 10
Match: AT1G19310.1 (RING/U-box superfamily protein )

HSP 1 Score: 105.5 bits (262), Expect = 7.3e-23
Identity = 46/89 (51.69%), Postives = 62/89 (69.66%), Query Frame = 0

Query: 82  FDCNICFDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHPQCPVCKADISHTTMVP 141
           F+CNIC D A +P+VTLCGHL+CWPC+YKWLH+ S S        CPVCKA I    +VP
Sbjct: 21  FECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQS------KDCPVCKAVIEEDRLVP 80

Query: 142 LYGRGQTAEEVEPEANSMHQDIDIPPRPS 171
           LYGRG+++   +P + S+   +++P RPS
Sbjct: 81  LYGRGKSS--ADPRSKSI-PGLEVPNRPS 100

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038881993.14.7e-12588.38E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881994.1 E3 u... [more]
XP_004134706.11.1e-12185.77E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus] >XP_031743482.1 ... [more]
XP_004134707.13.2e-12187.14E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743484.1 ... [more]
XP_023003183.16.0e-12085.54E3 ubiquitin-protein ligase RMA1H1-like isoform X1 [Cucurbita maxima] >XP_023003... [more]
XP_008439861.11.0e-11986.72PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >KAA0052689.1 ... [more]
Match NameE-valueIdentityDescription
Q6R5671.3e-4544.69E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=... [more]
Q8GUK71.9e-4442.61E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV... [more]
O644251.2e-3841.96E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV... [more]
P930309.6e-2834.53E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV... [more]
Q999421.2e-1743.43E3 ubiquitin-protein ligase RNF5 OS=Homo sapiens OX=9606 GN=RNF5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KK601.5e-12187.14Rnf5 OS=Cucumis sativus OX=3659 GN=Csa_6G517320 PE=4 SV=1[more]
A0A6J1KVR42.9e-12085.54E3 ubiquitin-protein ligase RMA1H1-like isoform X1 OS=Cucurbita maxima OX=3661 G... [more]
A0A5A7UGP75.0e-12086.72E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3AZB85.0e-12086.72E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo OX=3656 GN=LOC103484523 ... [more]
A0A6J1EI235.1e-11783.88E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT4G27470.11.4e-4542.61RING membrane-anchor 3 [more]
AT4G03510.18.5e-4041.96RING membrane-anchor 1 [more]
AT4G03510.28.5e-4041.96RING membrane-anchor 1 [more]
AT4G28270.16.8e-2934.53RING membrane-anchor 2 [more]
AT1G19310.17.3e-2351.69RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 84..130
e-value: 2.7E-8
score: 43.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 84..131
score: 12.415513
IPR018957Zinc finger, C3HC4 RING-typePFAMPF00097zf-C3HC4coord: 84..130
e-value: 4.8E-8
score: 32.7
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 66..165
e-value: 4.2E-16
score: 60.8
IPR045103E3 ubiquitin-protein ligase RNF5/RNF185-likePANTHERPTHR12313E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATEDcoord: 56..294
NoneNo IPR availablePANTHERPTHR12313:SF6E3 UBIQUITIN-PROTEIN LIGASE RMA3coord: 56..294
NoneNo IPR availableCDDcd16745RING-HC_AtRMA_likecoord: 82..132
e-value: 2.31533E-25
score: 93.5227
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 78..145
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 99..108

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0019254.1Sed0019254.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071712 ER-associated misfolded protein catabolic process
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0044390 ubiquitin-like protein conjugating enzyme binding
molecular_function GO:0061630 ubiquitin protein ligase activity