Sed0019002 (gene) Chayote v1

Overview
NameSed0019002
Typegene
OrganismSechium edule (Chayote v1)
Descriptionsubtilisin-like protease SBT6.1
LocationLG12: 34013414 .. 34020191 (+)
RNA-Seq ExpressionSed0019002
SyntenySed0019002
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAAGTTTAATCGGCCCGAAAAAGTTCAGAACTGCAAATTTCCATCGTCTTCTTCATACGCTCAACCCATCCTTCCTTTTCCCGCCAAATCAATGAATTCTCACAATTTCTTGTTGTCGCAATCATGATCGGTTCATCAATTTCCTTCATCATCATCATCTTCATCTCCATCTCGTTTTTCCAATTCAAACCTTCTTCCGATCACACCGTATTCCAAACCCTAGCTCGAAACCACTCACCGGCCGATCCATTTCCGGCCAACAATCTCACCCACGGGAACCGAGGCTCGCGTAACAAGAACTACATTGTTCGATTCGTTCAGTACAGGGAGGCGAAGGAGCATCGGTTCTACCTCGAATCGCGGGTTCGATCGGGCGGCTGGGGATGGATCGAGAGGCGGAACGCTGCGTCGAAGTACCCGACCGATTTCGGATTGGTTTCGATTGAGGAATCGGTGAGAAGAGAGCTGATGGAGGAGATTGAGGAGTTGGAGCTTGTTAAGGATGTGAATGTAGATGCAAGCCATGCTAGAGGATTGGTAACCGAGGATGGAGGTAGAGTTGGGGCTTTTGTTGATGGGAAGAAGCGTCCGGGGAAGATTTTCTCATCCATGTCGTTTAACGAAGAGGGCGGGGAACGTTATTCGGCCGCGATTAGCAATGCCTCTAATCGATGGGGACGGCATCTTTCGATGCAGGTAACTCTCTTTCAATGCTCAACTTGTTCTTTGCTTGTTGATCTATTTGGGTTTAGACTGTCAACTATGAGGAAAATGAAGTAGCAATGGGGATTTCATGTGTCTCATTGAATGAATTTGATACAGACTGATTTAATTTATCGTTGGAAGAGCTCTTGTTCAGTAATTAGATATATGGATTATTTATCTTTAATTTTGTTGATAAACGATTAAAAAAATATGCATCTATTTTCTCAATGATAGATTGAATTGTGGGATTCTTTGAGATTCCATTTAATCAATTTATTCGAAATTTGTAGGTCGGGTTATGTCCACCTTGACTATTCTCACGTGACTTGTCGTCGTACCGTACAACATTTGGATGTCAAGAAAACTTGTAGTTAGATGGCCACCATGGATTGAACTCATCACTTCTTTATTGCTTATAACACTGGATTATTTATCTTTGATAAGCCAAATTTCAATCATAACAACTGTAATGTATGTCTAGTTATATACATTTAGGATAGTTCAATATGGTAAACCAAATGTTTCCCTTCATTTCCAGTTTTAATTTTGGGTTCTAGTTAACTTTCACTGAGAAACAATTCTCGATTTCAGAGATCCCAAGTTACCTCATTATTTGGAGCAGACTCTCTTTGGTCAAAAGGGTATACTGGTTGCAAAGTCAAAATGGCTATATTCGATACAGGAATTCGTGCTAGTCACCCACATTTTAAGAATATCAAGGTAATAAAATCTTGGAATCTGTTTTCATTTGATGAGCATTTTATTTAGTTATTAGATTGAAAGAAAGTCTCCACCAATAGTGATTTATTTCACTTTGTAGATTATAATTTTCGATTTATTTTCAGGAGCGAACAAATTGGACCAATGAAGATACTTTAAATGATAATCTTGGACATGGGACATTTGTTGCTGGTGTCATTGCAGGTGGAGATGAAGAGTGTCTAGGATTTGCTCCAGATACAGAGATATATGCTTTTCGTGTGTTTACAGATGCACAGGTAATGCACATTGATCATTACTCAACAGTAACATGTTTGCTAGAGCTTTGACTTCAAAAAAAGTGTTTTATATCATTTTTCATTGGTTTATCTTCAAACATTGTGGAAAAATTAAGGAATTATTTTTATTTCTTTTTCATCTAAAAAAAGGATTATGAAAAGTATGTATTCAGCCTATTGCAGGTGTCATACACTTCATGGTTTCTCGATGCATTCAACTATGCAATTGCTACAAATATGGATGTCTTAAATTTAAGCATTGGTGGACCAGATTACTTGGATCTTCCATTTGTGGAGAAGGTAAGATTTCTTTGATCGGCTGATTGCAATTTGGTGATCTGGCAACATAAGTTATCAGCTTGAGAAAATATCTCTTAATTTTATTTGGTTTGAGGTTAATTAATTGCTTAATATGTTGTTTAAAAAAATGCTTAATATGTCTTGTTCGAACAAATACTTCATACATGTAAGTTTGCAAATAAATTTAACCGGTTAACTTTTTTTCTGGTTAGATTTTTCCAGCATTGCTATTACAAAAACCTTTATATTGATGAAATGTGTCATATTTGGGATACTGCGAATTTTCATTCTTCATGATATATATTGAAAGTTCTTGGACAGCCTACATAATCTGGCAGTTCTGTTTGCCTTACCTTAACATGATCATTAGTGAATATGCAGTTGAAGACTTTCACATGTGAATGAATCATCTCTTACTTGGTGAAGTTTAGTTTTTGGTTTCATTAATTAATGCATCTGTAGGTTAAACTTTTGGCCCTGGGCTTATGGTTGTAACATAATAATAGAGTAAGAGATATATATTTTAATCCATGTTCCATGCGTAAGAAATAATTACTTTCAATCAATTGAATGTCTATGCATTAGCCTTAACATAATCATGCTGGTGACCTCGATCTGGTGTGTTCAATTATGATGCCATCGTTTTAATTTAAACTTTTATTTGTCCTCAAGGGGTGACACAGTGGTTGAAGACTTGGGCTTTGCGGGTATGCTCTCCTCAAGATCCCAGGTTCGAGACTCAGCTGTGACATTACTTCTTCGATGTCTCCTGGTGCCTGGCCTAGAGACGGGCGTGGTTACCCTTGTTTCAAAAAAAAAAAAATGTTAACTTCTATTTTATTTCTCTACCAGATTTGGGAAATAACGGCAAATAATATCATCATGGTTTCTGCTATTGGAAATGATGGACCACTTTATGGAACTTTAAACAACCCTGCAGATCAGAGTGATGTAATTGGTGTTGGTGGTATTGACTACAATGATCATATTGCTTCATTTTCGTCACGTGGCATGACTACTTGGGAGATGCCTCATGGGTAAGTTACACTTTGCTCAGGGTCTTAAACTCCTTCAGTACTTTACTTCTATCCCTCTTAAGTTATCCTCTAGAAATTTCAAAATATTTCAGCTATTGATTCCTTGGTAATATTGGGCAGATGATTTGTCTCATTTCTAGGGTTGGAATTTGGCCTGGGTGAAAGTAGATATGTTGGATGCATTGAAATTCAAACTGAGTCCATAGGAATTTAACTTCTTATCCAATATATTGATTTATTTTCCCCTAATTAGTACTTGTTTTTTTATTTTTTTTTATCTTTCTCCCACTTTCTTTCCAACCACATCGGCACTCTCAAACATGTCATTTTGTACAGTTATGGTCGTGTGAAGCCTGATGTTGTTGCATATGGTCGGGAAATTATGGGGTCCAAAATTAGTACTGGGTGCAAAAGTTTATCTGGCACTAGTGTGGCGAGCCCTGTTGTTGCTGGTGTGGTATGTCTACTTGTTAGTGTCATCCCCGAAAGCCAGCGGAAGATTATTTTAAATCCAGCTAGTATGAAGCAAGCACTTGTTGAGGGAGCTGCTAAGCTTGCTGGTCCTAATTTGTACGAGCAAGGTGCTGGCAGAGTTAATCTGTAGGTTTCAACTCTGGTTGTTGTTGTTGTTGTTTTATACTTTGCCTCTTAGAGTGTGTTCTTATTTCTCTGATTTTTCCAGGTTAGAATCGTATGAAATACTTAAGAGCTACCAACCCCGGGCAAGCATCTTCCCTAGTGTTCTTGACTATACAGATTGCCCTTATTCGTGGCCTTTCTGTCGCCAACCTCTTTATGCAGGTGCAATGCCGATTATCTTCAATGCTACTATTTTGAATGGGATGGGTGTAATTGGCTATGTTGAAAGTCAACCAACTTGGCATCCTTTGAATGATGAAGGAAATCTGTTAAGCATTCACTTCACTTATTCTCAAGTCATCTGGCCTTGGACTGGTTATATTGCACTTCACATGCAAATTAAGGAAGAAGGTGCACAGTTTTCTGGAGAAATTGAGGGTAATGTGACTCTAACAGTATACAGTCCATCTAGTGGAGAGAAGAATCGCCAGACTAGTACTTGTGTGCTGCAACTGAAGTTAAAAGTGGTTCCAACACCTCCAAGATCCAAACGAGTTTTATGGGATCAATTTCATAGTATCAAATATCCTCCTGGTTATATTCCGAGGGACTCTTTGGATGTACGCAACGACATTCTGGACTGGCATGGAGACCACCTTCATACGAATTTTCATATCATGTTTAACATGTTAAGAGATGCTGGATACTATGTTGAATCGCTTGGTTCGCCTCTTACATGCTTCGATGCTCGCCAATATGGGACACTTCTACTAGTGGATCTAGAAGACGAGTACTTCAAAGAGGAAATCGAAAAGCTGAGGGACGATGTTATGATTACTGGATTGGGATTGGCTGTGTTCTCTGAATGGTATAATGTAGAAACAATGGTAAAAATGAGGTTCTTTGATGATAACACAAGAAGCTGGTGGACTCCAGTTACTGGAGGTGCAAATATTCCTGCACTAAATGATCTCTTAGCCCCATTTGGTATTGCTTTCGGGGATAAGATTTTGAATGGTGATTTTTCTATTGATGGCGAACAGAGTCGATATGCATCAGGAACAGATATTGTGAGGTTTCCACAAGGTGGTTATGTGCATAGTTTTCCTTTCGTTGATAGCTCAGAGAGTGGAGTGGCTCAGAGCATACTAACCTCTAGCATGAATAAGGTAACTGTCACAAGCTTCTCTATGAATTTCTCTTTGTAAATAGTTTTTATGTGGACCAAAAGCTGATCCAAAATATACTTCTGGTCCAGTTTCCTTTTTTAGAAGTTCAAGTAATAATGGAGAACTCTGTATTTTAGTTGAATTAGATACTTAAGGGGGTGTTTGGTGCAAGGGAAATGAGAATGATTATGAGGAAGATGAGAAATAGGAGGAAAATATGAGAGAGAATGATTATCGGTGAGAATGGTTGTAGGAGAGAATAATTATTTGTATTTGGAGTGAAGAGAATAATTATGGGGAGAACCATTACTTTGTGTTTGGAGTGAACAGAATAAAAATAGTGAGGATGGGGGAATAAAAAGTGGTTATGGGAGGATGAAAAGTGGTTATGGAAAAAATGAGATTATGGGGGAATGATTCTTGGTGGACCAAACATAAATATGAGGGTCATTCTCTCCATAATCATTATGATCCTCCTCCCTCAAACACCCCTAAATGTTGATTTGATATCTTACACAAGTGGGAATTAGGAAGTTGTAGCAGCAGAAGCATATCTATTTTCTTTTGCATGAATATGGTAAGAACCCGAACTCTATTATAATATCGAAATAATGAAATTATTTAAGGTACTTAGGACCCTCCTATTCTTGAGATCACTCAAGACCCAATACCACAGTGGCCAAACTACTCGAGGCCTTTCCCTCTACCCACACCCTTTATTTATAACCAAATTCACTAACAAACTCTCTAATTAATTGCCCATATACTCCTACATTAATCTTAATTCTTCTAGATGCCCAACCCTATCGCCACAATGGTGCTTGAGAAGTTAGTTTCTTGATTCCAATGAATGGAGCACGAGAAACACCTGTTTATCATTAAATTTAGGCAAACTTTACGATTATTCATAGTTTCATGAAATTATTATGGTCCGTGTAACATGATAAAACTAACTGTTTATCAACAAATGTAGGCGGAGTTTCCGATTCTCGGTCTATTAGAGGCGGTAGAGGGCCGCATTGCAGTCTATGGAGATTCAAATTGTCTGGACAGCAGCCATATGGTTACAAATTGCTACTGGCTCCTGAAAAAAATATTAGATTTCACCAGTGGAAATATTAGAGATCCGGTGCTCTTCACCAAGTCTTCGAAACGAAATTCACCTCTGTATCTAGAAGATAACAAACTACCATCTCGTAGAAGCGATGTAAATTTCTCTACTTATTCTGCTGTTGTTGGAAAGGAATTGATATGCAGGAGCGATTCGAGGTTTGAAGTATGGGGGACCAAGGGATACAGTATACATGTCAGGGGAAGAAATAGAAGGTTGCCCGGATTTCCCGTATTTGACTTGGGAAGAGGCTTGAATTATTCTACTTCGACGAGACCTCCCAAGTTGACTTTAAAAGACCGAACCGATACGTACGCAAACCGATACTTAAGCTTATTCTACCGGGATGAGGTGAGAGTTGACTTCCCAATGAATTGGGACTTAAATTACAATATTATTTCATCAATATGATTCTTCTAATTTTCTATCTATTGTTTTGTTCTTGCTTATAGCCTGGCGCTCCTTTGATAGTTCCTAATCATTGGCTTGTTCCTGCTATAGTTGCATTGACAGGTCAGTTATATTATATTCCTCATTGCTCTTGAATTGAGTTTCCTTTTCTGAATTGTATGCCTTACTTGGTTCCTTTTTTCTTATTAGGTCTTTTGCTCATTTTGAGTTTTTGGCGGATTAGACAGAAGCGTCGGCGGCGACGAAGAGGCTCGGGATCCGCACGTTTTCCCAATTTATAGTTGGCAGGACTTTTGATTAGTTTTCCAAGTATAACATATACTCTTCCATGAAAGAATTTTGATGTAAAATCTAGACGCTTTAATATTGTTTCTACAGTAATACATGTGTTGCAAATGAGAATTGAAATTTTGAAGGTGATTAATGTGTTGTTTGGTGATCCCAGGAAGCAAGGTTCTAATTACTA

mRNA sequence

GAGAAGTTTAATCGGCCCGAAAAAGTTCAGAACTGCAAATTTCCATCGTCTTCTTCATACGCTCAACCCATCCTTCCTTTTCCCGCCAAATCAATGAATTCTCACAATTTCTTGTTGTCGCAATCATGATCGGTTCATCAATTTCCTTCATCATCATCATCTTCATCTCCATCTCGTTTTTCCAATTCAAACCTTCTTCCGATCACACCGTATTCCAAACCCTAGCTCGAAACCACTCACCGGCCGATCCATTTCCGGCCAACAATCTCACCCACGGGAACCGAGGCTCGCGTAACAAGAACTACATTGTTCGATTCGTTCAGTACAGGGAGGCGAAGGAGCATCGGTTCTACCTCGAATCGCGGGTTCGATCGGGCGGCTGGGGATGGATCGAGAGGCGGAACGCTGCGTCGAAGTACCCGACCGATTTCGGATTGGTTTCGATTGAGGAATCGGTGAGAAGAGAGCTGATGGAGGAGATTGAGGAGTTGGAGCTTGTTAAGGATGTGAATGTAGATGCAAGCCATGCTAGAGGATTGGTAACCGAGGATGGAGGTAGAGTTGGGGCTTTTGTTGATGGGAAGAAGCGTCCGGGGAAGATTTTCTCATCCATGTCGTTTAACGAAGAGGGCGGGGAACGTTATTCGGCCGCGATTAGCAATGCCTCTAATCGATGGGGACGGCATCTTTCGATGCAGAGATCCCAAGTTACCTCATTATTTGGAGCAGACTCTCTTTGGTCAAAAGGGTATACTGGTTGCAAAGTCAAAATGGCTATATTCGATACAGGAATTCGTGCTAGTCACCCACATTTTAAGAATATCAAGGAGCGAACAAATTGGACCAATGAAGATACTTTAAATGATAATCTTGGACATGGGACATTTGTTGCTGGTGTCATTGCAGGTGGAGATGAAGAGTGTCTAGGATTTGCTCCAGATACAGAGATATATGCTTTTCGTGTGTTTACAGATGCACAGGTGTCATACACTTCATGGTTTCTCGATGCATTCAACTATGCAATTGCTACAAATATGGATGTCTTAAATTTAAGCATTGGTGGACCAGATTACTTGGATCTTCCATTTGTGGAGAAGATTTGGGAAATAACGGCAAATAATATCATCATGGTTTCTGCTATTGGAAATGATGGACCACTTTATGGAACTTTAAACAACCCTGCAGATCAGAGTGATGTAATTGGTGTTGGTGGTATTGACTACAATGATCATATTGCTTCATTTTCGTCACGTGGCATGACTACTTGGGAGATGCCTCATGGTTATGGTCGTGTGAAGCCTGATGTTGTTGCATATGGTCGGGAAATTATGGGGTCCAAAATTAGTACTGGGTGCAAAAGTTTATCTGGCACTAGTGTGGCGAGCCCTGTTGTTGCTGGTGTGGTATGTCTACTTGTTAGTGTCATCCCCGAAAGCCAGCGGAAGATTATTTTAAATCCAGCTAGTATGAAGCAAGCACTTGTTGAGGGAGCTGCTAAGCTTGCTGGTCCTAATTTGTACGAGCAAGGTGCTGGCAGAGTTAATCTGTTAGAATCGTATGAAATACTTAAGAGCTACCAACCCCGGGCAAGCATCTTCCCTAGTGTTCTTGACTATACAGATTGCCCTTATTCGTGGCCTTTCTGTCGCCAACCTCTTTATGCAGGTGCAATGCCGATTATCTTCAATGCTACTATTTTGAATGGGATGGGTGTAATTGGCTATGTTGAAAGTCAACCAACTTGGCATCCTTTGAATGATGAAGGAAATCTGTTAAGCATTCACTTCACTTATTCTCAAGTCATCTGGCCTTGGACTGGTTATATTGCACTTCACATGCAAATTAAGGAAGAAGGTGCACAGTTTTCTGGAGAAATTGAGGGTAATGTGACTCTAACAGTATACAGTCCATCTAGTGGAGAGAAGAATCGCCAGACTAGTACTTGTGTGCTGCAACTGAAGTTAAAAGTGGTTCCAACACCTCCAAGATCCAAACGAGTTTTATGGGATCAATTTCATAGTATCAAATATCCTCCTGGTTATATTCCGAGGGACTCTTTGGATGTACGCAACGACATTCTGGACTGGCATGGAGACCACCTTCATACGAATTTTCATATCATGTTTAACATGTTAAGAGATGCTGGATACTATGTTGAATCGCTTGGTTCGCCTCTTACATGCTTCGATGCTCGCCAATATGGGACACTTCTACTAGTGGATCTAGAAGACGAGTACTTCAAAGAGGAAATCGAAAAGCTGAGGGACGATGTTATGATTACTGGATTGGGATTGGCTGTGTTCTCTGAATGGTATAATGTAGAAACAATGGTAAAAATGAGGTTCTTTGATGATAACACAAGAAGCTGGTGGACTCCAGTTACTGGAGGTGCAAATATTCCTGCACTAAATGATCTCTTAGCCCCATTTGGTATTGCTTTCGGGGATAAGATTTTGAATGGTGATTTTTCTATTGATGGCGAACAGAGTCGATATGCATCAGGAACAGATATTGTGAGGTTTCCACAAGGTGGTTATGTGCATAGTTTTCCTTTCGTTGATAGCTCAGAGAGTGGAGTGGCTCAGAGCATACTAACCTCTAGCATGAATAAGGCGGAGTTTCCGATTCTCGGTCTATTAGAGGCGGTAGAGGGCCGCATTGCAGTCTATGGAGATTCAAATTGTCTGGACAGCAGCCATATGGTTACAAATTGCTACTGGCTCCTGAAAAAAATATTAGATTTCACCAGTGGAAATATTAGAGATCCGGTGCTCTTCACCAAGTCTTCGAAACGAAATTCACCTCTGTATCTAGAAGATAACAAACTACCATCTCGTAGAAGCGATGTAAATTTCTCTACTTATTCTGCTGTTGTTGGAAAGGAATTGATATGCAGGAGCGATTCGAGGTTTGAAGTATGGGGGACCAAGGGATACAGTATACATGTCAGGGGAAGAAATAGAAGGTTGCCCGGATTTCCCGTATTTGACTTGGGAAGAGGCTTGAATTATTCTACTTCGACGAGACCTCCCAAGTTGACTTTAAAAGACCGAACCGATACGTACGCAAACCGATACTTAAGCTTATTCTACCGGGATGAGCCTGGCGCTCCTTTGATAGTTCCTAATCATTGGCTTGTTCCTGCTATAGTTGCATTGACAGGTCTTTTGCTCATTTTGAGTTTTTGGCGGATTAGACAGAAGCGTCGGCGGCGACGAAGAGGCTCGGGATCCGCACGTTTTCCCAATTTATAGTTGGCAGGACTTTTGATTAGTTTTCCAAGTATAACATATACTCTTCCATGAAAGAATTTTGATGTAAAATCTAGACGCTTTAATATTGTTTCTACAGTAATACATGTGTTGCAAATGAGAATTGAAATTTTGAAGGTGATTAATGTGTTGTTTGGTGATCCCAGGAAGCAAGGTTCTAATTACTA

Coding sequence (CDS)

ATGATCGGTTCATCAATTTCCTTCATCATCATCATCTTCATCTCCATCTCGTTTTTCCAATTCAAACCTTCTTCCGATCACACCGTATTCCAAACCCTAGCTCGAAACCACTCACCGGCCGATCCATTTCCGGCCAACAATCTCACCCACGGGAACCGAGGCTCGCGTAACAAGAACTACATTGTTCGATTCGTTCAGTACAGGGAGGCGAAGGAGCATCGGTTCTACCTCGAATCGCGGGTTCGATCGGGCGGCTGGGGATGGATCGAGAGGCGGAACGCTGCGTCGAAGTACCCGACCGATTTCGGATTGGTTTCGATTGAGGAATCGGTGAGAAGAGAGCTGATGGAGGAGATTGAGGAGTTGGAGCTTGTTAAGGATGTGAATGTAGATGCAAGCCATGCTAGAGGATTGGTAACCGAGGATGGAGGTAGAGTTGGGGCTTTTGTTGATGGGAAGAAGCGTCCGGGGAAGATTTTCTCATCCATGTCGTTTAACGAAGAGGGCGGGGAACGTTATTCGGCCGCGATTAGCAATGCCTCTAATCGATGGGGACGGCATCTTTCGATGCAGAGATCCCAAGTTACCTCATTATTTGGAGCAGACTCTCTTTGGTCAAAAGGGTATACTGGTTGCAAAGTCAAAATGGCTATATTCGATACAGGAATTCGTGCTAGTCACCCACATTTTAAGAATATCAAGGAGCGAACAAATTGGACCAATGAAGATACTTTAAATGATAATCTTGGACATGGGACATTTGTTGCTGGTGTCATTGCAGGTGGAGATGAAGAGTGTCTAGGATTTGCTCCAGATACAGAGATATATGCTTTTCGTGTGTTTACAGATGCACAGGTGTCATACACTTCATGGTTTCTCGATGCATTCAACTATGCAATTGCTACAAATATGGATGTCTTAAATTTAAGCATTGGTGGACCAGATTACTTGGATCTTCCATTTGTGGAGAAGATTTGGGAAATAACGGCAAATAATATCATCATGGTTTCTGCTATTGGAAATGATGGACCACTTTATGGAACTTTAAACAACCCTGCAGATCAGAGTGATGTAATTGGTGTTGGTGGTATTGACTACAATGATCATATTGCTTCATTTTCGTCACGTGGCATGACTACTTGGGAGATGCCTCATGGTTATGGTCGTGTGAAGCCTGATGTTGTTGCATATGGTCGGGAAATTATGGGGTCCAAAATTAGTACTGGGTGCAAAAGTTTATCTGGCACTAGTGTGGCGAGCCCTGTTGTTGCTGGTGTGGTATGTCTACTTGTTAGTGTCATCCCCGAAAGCCAGCGGAAGATTATTTTAAATCCAGCTAGTATGAAGCAAGCACTTGTTGAGGGAGCTGCTAAGCTTGCTGGTCCTAATTTGTACGAGCAAGGTGCTGGCAGAGTTAATCTGTTAGAATCGTATGAAATACTTAAGAGCTACCAACCCCGGGCAAGCATCTTCCCTAGTGTTCTTGACTATACAGATTGCCCTTATTCGTGGCCTTTCTGTCGCCAACCTCTTTATGCAGGTGCAATGCCGATTATCTTCAATGCTACTATTTTGAATGGGATGGGTGTAATTGGCTATGTTGAAAGTCAACCAACTTGGCATCCTTTGAATGATGAAGGAAATCTGTTAAGCATTCACTTCACTTATTCTCAAGTCATCTGGCCTTGGACTGGTTATATTGCACTTCACATGCAAATTAAGGAAGAAGGTGCACAGTTTTCTGGAGAAATTGAGGGTAATGTGACTCTAACAGTATACAGTCCATCTAGTGGAGAGAAGAATCGCCAGACTAGTACTTGTGTGCTGCAACTGAAGTTAAAAGTGGTTCCAACACCTCCAAGATCCAAACGAGTTTTATGGGATCAATTTCATAGTATCAAATATCCTCCTGGTTATATTCCGAGGGACTCTTTGGATGTACGCAACGACATTCTGGACTGGCATGGAGACCACCTTCATACGAATTTTCATATCATGTTTAACATGTTAAGAGATGCTGGATACTATGTTGAATCGCTTGGTTCGCCTCTTACATGCTTCGATGCTCGCCAATATGGGACACTTCTACTAGTGGATCTAGAAGACGAGTACTTCAAAGAGGAAATCGAAAAGCTGAGGGACGATGTTATGATTACTGGATTGGGATTGGCTGTGTTCTCTGAATGGTATAATGTAGAAACAATGGTAAAAATGAGGTTCTTTGATGATAACACAAGAAGCTGGTGGACTCCAGTTACTGGAGGTGCAAATATTCCTGCACTAAATGATCTCTTAGCCCCATTTGGTATTGCTTTCGGGGATAAGATTTTGAATGGTGATTTTTCTATTGATGGCGAACAGAGTCGATATGCATCAGGAACAGATATTGTGAGGTTTCCACAAGGTGGTTATGTGCATAGTTTTCCTTTCGTTGATAGCTCAGAGAGTGGAGTGGCTCAGAGCATACTAACCTCTAGCATGAATAAGGCGGAGTTTCCGATTCTCGGTCTATTAGAGGCGGTAGAGGGCCGCATTGCAGTCTATGGAGATTCAAATTGTCTGGACAGCAGCCATATGGTTACAAATTGCTACTGGCTCCTGAAAAAAATATTAGATTTCACCAGTGGAAATATTAGAGATCCGGTGCTCTTCACCAAGTCTTCGAAACGAAATTCACCTCTGTATCTAGAAGATAACAAACTACCATCTCGTAGAAGCGATGTAAATTTCTCTACTTATTCTGCTGTTGTTGGAAAGGAATTGATATGCAGGAGCGATTCGAGGTTTGAAGTATGGGGGACCAAGGGATACAGTATACATGTCAGGGGAAGAAATAGAAGGTTGCCCGGATTTCCCGTATTTGACTTGGGAAGAGGCTTGAATTATTCTACTTCGACGAGACCTCCCAAGTTGACTTTAAAAGACCGAACCGATACGTACGCAAACCGATACTTAAGCTTATTCTACCGGGATGAGCCTGGCGCTCCTTTGATAGTTCCTAATCATTGGCTTGTTCCTGCTATAGTTGCATTGACAGGTCTTTTGCTCATTTTGAGTTTTTGGCGGATTAGACAGAAGCGTCGGCGGCGACGAAGAGGCTCGGGATCCGCACGTTTTCCCAATTTATAG

Protein sequence

MIGSSISFIIIIFISISFFQFKPSSDHTVFQTLARNHSPADPFPANNLTHGNRGSRNKNYIVRFVQYREAKEHRFYLESRVRSGGWGWIERRNAASKYPTDFGLVSIEESVRRELMEEIEELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAAISNASNRWGRHLSMQRSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHPHFKNIKERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQGAGRVNLLESYEILKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGMGVIGYVESQPTWHPLNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPSSGEKNRQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHLHTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMITGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILTSSMNKAEFPILGLLEAVEGRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDPVLFTKSSKRNSPLYLEDNKLPSRRSDVNFSTYSAVVGKELICRSDSRFEVWGTKGYSIHVRGRNRRLPGFPVFDLGRGLNYSTSTRPPKLTLKDRTDTYANRYLSLFYRDEPGAPLIVPNHWLVPAIVALTGLLLILSFWRIRQKRRRRRRGSGSARFPNL
Homology
BLAST of Sed0019002 vs. NCBI nr
Match: XP_038879333.1 (subtilisin-like protease SBT6.1 [Benincasa hispida])

HSP 1 Score: 1952.2 bits (5056), Expect = 0.0e+00
Identity = 956/1046 (91.40%), Postives = 997/1046 (95.32%), Query Frame = 0

Query: 4    SSISFIII---IFISISFFQFKPSSDHTVFQTLARNHSPADPFPANNLTHGNRGSRNKNY 63
            SSISFI+I   IFISIS FQFKPSSDHTVFQTL RN+SP DP P+ NLT GN     K+Y
Sbjct: 5    SSISFIVIFVPIFISISLFQFKPSSDHTVFQTLTRNYSPVDPSPSKNLTDGN--VLRKSY 64

Query: 64   IVRFVQYREAKEHRFYLESRVRSGGWGWIERRNAASKYPTDFGLVSIEESVRRELMEEIE 123
            IVRF+QYREAKEHRFYLESR+RSGGW WIERRN ASKYPTDFGLVSIE+SVR EL++EIE
Sbjct: 65   IVRFLQYREAKEHRFYLESRIRSGGWEWIERRNPASKYPTDFGLVSIEDSVRGELIKEIE 124

Query: 124  ELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAAISNA 183
            ELE VKDVNVDA+H RGL+ EDGGRVGAFVDGKKRPGKIF+SMSF E GGERY+ AISNA
Sbjct: 125  ELEFVKDVNVDATHVRGLLMEDGGRVGAFVDGKKRPGKIFTSMSFKEGGGERYT-AISNA 184

Query: 184  SNRWGRHLSMQRSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHPHFKNIKERTNWT 243
            SN WGRHLSM+RSQVTSLFGADSLW+KGYTG KVKMAIFDTGIR++HPHF+NIKERTNWT
Sbjct: 185  SNGWGRHLSMERSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRSNHPHFRNIKERTNWT 244

Query: 244  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 303
            NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI
Sbjct: 245  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 304

Query: 304  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 363
            ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG
Sbjct: 305  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 364

Query: 364  VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 423
            VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS
Sbjct: 365  VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 424

Query: 424  PVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQGAGRVNLLESYEI 483
            PVVAGVVCLLVSVIPESQRK+ILNPASMKQALVEGAAKLAGPN+YEQGAGRV+LLESYEI
Sbjct: 425  PVVAGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEI 484

Query: 484  LKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGMGVIGYVESQPTW 543
            LKSYQPRASIFP VLDYTDCPY+WPFCRQPLYAGAMPIIFNATILNGMGVIGYVE QPTW
Sbjct: 485  LKSYQPRASIFPDVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTW 544

Query: 544  HPLNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS-PSSGE 603
            HPL++EGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS PS GE
Sbjct: 545  HPLDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGE 604

Query: 604  KNRQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHL 663
            KNR+ STCVLQLKLKVVPTPPRSKR+LWDQFH+IKYPPGYIPRDSLDVRNDILDWHGDHL
Sbjct: 605  KNRRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHL 664

Query: 664  HTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 723
            HTNFHIMFNML DAGYYVE+LGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT
Sbjct: 665  HTNFHIMFNMLSDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 724

Query: 724  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 783
            GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG
Sbjct: 725  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 784

Query: 784  DFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILTSSMNKAEFPILGLLE 843
            DFSIDGEQSR+ASGTDIVRFPQGGYVHSFPFVDSSESG AQSILTSSM+KA+FPILGLLE
Sbjct: 785  DFSIDGEQSRFASGTDIVRFPQGGYVHSFPFVDSSESGAAQSILTSSMSKADFPILGLLE 844

Query: 844  AVEGRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDPVLFTKSSKRNSPLYLEDN 903
            A+EGR+AVYGDSNCLDSSHMVTNCYWLL+KILDFTSGNIRDPVLFTK SKR+SPLYLEDN
Sbjct: 845  AIEGRVAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKRDSPLYLEDN 904

Query: 904  KLPSRRSDVNFSTYSAVVGKELICRSDSRFEVWGTKGYSIHVRGRNRRLPGFPVFDLGRG 963
            KLPSRRSDVNFS YSAV GKEL+CRSDSRFEVWGTKGYSI VRGRNRRLPGFPV DLGRG
Sbjct: 905  KLPSRRSDVNFSLYSAVAGKELLCRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRG 964

Query: 964  LN---YSTSTRPPKLTLKDRTDTYANRYLSLFYRDEPGAPLIVPNHWLVPAIVALTGLLL 1023
            LN    S+S  PPKLTLKDR DTY NRYLSLFYRDEP  PLIVPN WLVPA+VALTGLLL
Sbjct: 965  LNSTLESSSMGPPKLTLKDRRDTYGNRYLSLFYRDEPDMPLIVPNQWLVPAVVALTGLLL 1024

Query: 1024 ILSFWRIRQKRRRRRRGSGSARFPNL 1043
            +LSFWRIRQKRRRRRRGSGSARF N+
Sbjct: 1025 LLSFWRIRQKRRRRRRGSGSARFSNI 1047

BLAST of Sed0019002 vs. NCBI nr
Match: KAG6589917.1 (Subtilisin-like protease 6.1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023586.1 Subtilisin-like protease SBT6.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1936.4 bits (5015), Expect = 0.0e+00
Identity = 953/1047 (91.02%), Postives = 993/1047 (94.84%), Query Frame = 0

Query: 4    SSISFIII---IFISISFFQFKPSSDHTVFQTLARNHSPADPFPANNLTHGNRGSRNKNY 63
            SSISF +I   IFISIS FQF PSSDHTVFQTL RN+SP DPF + N+T GNR S+ KNY
Sbjct: 9    SSISFFVIFVPIFISISVFQFNPSSDHTVFQTLTRNYSPNDPFTSKNVTDGNRVSQ-KNY 68

Query: 64   IVRFVQYREAKEHRFYLESRVRSGGWGWIERRNAASKYPTDFGLVSIEESVRRELMEEIE 123
            IVRFVQYREAKEHRFYLESR+RSGGW WIERRN ASKYPTDFGLVSIE+SV REL+EEIE
Sbjct: 69   IVRFVQYREAKEHRFYLESRIRSGGWKWIERRNPASKYPTDFGLVSIEDSVVRELVEEIE 128

Query: 124  ELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAAISNA 183
            ELELVKDVNVDASH RGL+ E GGR+GAFVDGKKRPGKIF+SMSFNEEGGERY+ AISNA
Sbjct: 129  ELELVKDVNVDASHVRGLLAEGGGRIGAFVDGKKRPGKIFTSMSFNEEGGERYT-AISNA 188

Query: 184  SNRWGRHLSMQRSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHPHFKNIKERTNWT 243
            SNRWGRHL MQRSQVTSLFGADSLW+KGYTG KVKMAIFDTGIRA+HPHF+NIKERTNWT
Sbjct: 189  SNRWGRHLLMQRSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIKERTNWT 248

Query: 244  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 303
            NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI
Sbjct: 249  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 308

Query: 304  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 363
            ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG
Sbjct: 309  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 368

Query: 364  VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 423
            VGGIDYNDHIASFSSRGMTTWEMPHG GRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS
Sbjct: 369  VGGIDYNDHIASFSSRGMTTWEMPHGNGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 428

Query: 424  PVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQGAGRVNLLESYEI 483
            PVVAGVVCLLVSVIPESQRK ILNPASMKQALVEGAAKLAGPN+YEQGAGRV+LLESYEI
Sbjct: 429  PVVAGVVCLLVSVIPESQRKTILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEI 488

Query: 484  LKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGMGVIGYVESQPTW 543
            LKSYQPRASIFP VLDYTDCPY+WPFCRQPLYAGAMPIIFNATILNGMGVIGYVE QPTW
Sbjct: 489  LKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTW 548

Query: 544  HPLNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS-PSSGE 603
            +P ++EGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS PS GE
Sbjct: 549  NPSDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGE 608

Query: 604  KNRQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHL 663
            KNR+ STCVLQLKLKVVPTPPRSKR+LWDQFH+IKYPPGYIPRDSLDVRNDILDWHGDHL
Sbjct: 609  KNRRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHL 668

Query: 664  HTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 723
            HTNFHIMFNMLRDAGYYVE+LGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT
Sbjct: 669  HTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 728

Query: 724  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 783
            GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG
Sbjct: 729  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 788

Query: 784  DFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILTSSMNKAEFPILGLLE 843
            DFSIDGEQSRYASGTDIVRFP+GGYVHSFPF+DSSESG AQSILTSS++KA+FPILGLLE
Sbjct: 789  DFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQSILTSSLSKADFPILGLLE 848

Query: 844  AVEGRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDPVLFTKSSKRNSPLYLEDN 903
            A EGRIAVYGDSNCLDSSHMVTNCYWLL+KILDFTSGNIRDPVLFTK SK+NSPLYLEDN
Sbjct: 849  AAEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKQNSPLYLEDN 908

Query: 904  KLPSRRSDVNFSTYSAVVGKELICRSDSRFEVWGTKGYSIHVRGRNRRLPGFPVFDLGRG 963
            KLPSRRSDVNFS YSAV GKELIC SDSRFEVWGTKGYS+  RGRNRRLPGFP+ DLGR 
Sbjct: 909  KLPSRRSDVNFSLYSAVSGKELICMSDSRFEVWGTKGYSVQGRGRNRRLPGFPLIDLGRD 968

Query: 964  LN---YSTSTRPPKLTLKDRTDTYANRYLSLFYRDEPGAPLIVPNHWLVPAIVALTGLLL 1023
            LN    S+   PPKLTLKDR+D Y NRYLSLFYRDEP  PLIVPNHWLVPA+VA+ GLLL
Sbjct: 969  LNSTPESSLMGPPKLTLKDRSDKYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVAVAGLLL 1028

Query: 1024 ILSFWRIRQKRRRRRRGSGS-ARFPNL 1043
            +LSFWRIRQKRRRRRRGSGS ARF NL
Sbjct: 1029 LLSFWRIRQKRRRRRRGSGSAARFSNL 1053

BLAST of Sed0019002 vs. NCBI nr
Match: XP_023516371.1 (subtilisin-like protease SBT6.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1934.5 bits (5010), Expect = 0.0e+00
Identity = 952/1047 (90.93%), Postives = 992/1047 (94.75%), Query Frame = 0

Query: 4    SSISFIII---IFISISFFQFKPSSDHTVFQTLARNHSPADPFPANNLTHGNRGSRNKNY 63
            SSISF +I   IFISIS FQF PSSDHTVFQTL RN+SP DPFP+ N+T GNR S+ KNY
Sbjct: 9    SSISFFVIFVPIFISISIFQFNPSSDHTVFQTLTRNYSPNDPFPSKNVTDGNRVSQ-KNY 68

Query: 64   IVRFVQYREAKEHRFYLESRVRSGGWGWIERRNAASKYPTDFGLVSIEESVRRELMEEIE 123
            IVRFVQYREAKEHRFYLESR+RSGGW WIERRN ASKYPTDFGLVSIE+SV REL+EEIE
Sbjct: 69   IVRFVQYREAKEHRFYLESRIRSGGWKWIERRNPASKYPTDFGLVSIEDSVVRELVEEIE 128

Query: 124  ELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAAISNA 183
            ELELVKDVNVDASH RGL+ E GGR+GAFVDGKKRPGKIF+SMSFNEEGGERY+ AISNA
Sbjct: 129  ELELVKDVNVDASHVRGLLEEGGGRIGAFVDGKKRPGKIFTSMSFNEEGGERYT-AISNA 188

Query: 184  SNRWGRHLSMQRSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHPHFKNIKERTNWT 243
            SNRWGRHL MQRSQVTSLFGADSLW+KGYTG KVKMAIFDTGIRA+HPHF+NIKERTNWT
Sbjct: 189  SNRWGRHLLMQRSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIKERTNWT 248

Query: 244  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 303
            NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI
Sbjct: 249  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 308

Query: 304  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 363
            ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG
Sbjct: 309  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 368

Query: 364  VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 423
            VGGIDYNDHIASFSSRGMTTWEMPHG GRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS
Sbjct: 369  VGGIDYNDHIASFSSRGMTTWEMPHGNGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 428

Query: 424  PVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQGAGRVNLLESYEI 483
            PVVAGVVCLLVSVIPESQRK ILNPASMKQALVEGAAKL GPN+YEQGAGRV+LLESYEI
Sbjct: 429  PVVAGVVCLLVSVIPESQRKTILNPASMKQALVEGAAKLVGPNMYEQGAGRVDLLESYEI 488

Query: 484  LKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGMGVIGYVESQPTW 543
            LKSYQPRASIFP VLDYTDCPY+WPFCRQPLYAGAMPIIFNATILNGMGVIGYVE QPTW
Sbjct: 489  LKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTW 548

Query: 544  HPLNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS-PSSGE 603
            +P ++EGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS PS GE
Sbjct: 549  NPSDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGE 608

Query: 604  KNRQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHL 663
            KNR+ STCVLQLKLKVVPTPPRSKR+LWDQFH+IKYPPGYIPRDSLDVRNDILDWHGDHL
Sbjct: 609  KNRRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHL 668

Query: 664  HTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 723
            HTNFHIMFNMLRDAGYYVE+LGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT
Sbjct: 669  HTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 728

Query: 724  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 783
            GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG
Sbjct: 729  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 788

Query: 784  DFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILTSSMNKAEFPILGLLE 843
            DFSIDGEQSRYASGTDIVRFP+GGYVHSFPF+DSSESG AQSILTSS++KA+FPILGLLE
Sbjct: 789  DFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQSILTSSLSKADFPILGLLE 848

Query: 844  AVEGRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDPVLFTKSSKRNSPLYLEDN 903
            A EGRIAVYGDSNCLDSSHMVTNCYWLL+KILDFTSGNIRDPVLFTK SK+NSPLYLEDN
Sbjct: 849  AGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKQNSPLYLEDN 908

Query: 904  KLPSRRSDVNFSTYSAVVGKELICRSDSRFEVWGTKGYSIHVRGRNRRLPGFPVFDLGRG 963
            KLPSRRSDVNFS YSAV GKELIC SDSRFEVWGTKGYS+  RGRNRRLPGFP+ DLGR 
Sbjct: 909  KLPSRRSDVNFSLYSAVSGKELICMSDSRFEVWGTKGYSVQGRGRNRRLPGFPLIDLGRD 968

Query: 964  LN---YSTSTRPPKLTLKDRTDTYANRYLSLFYRDEPGAPLIVPNHWLVPAIVALTGLLL 1023
             N    S+   PPKLTLKDR+D Y NRYLSLFYRDEP  PLIVPNHWLVPA+VA+ GLLL
Sbjct: 969  SNSTPESSLMGPPKLTLKDRSDKYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVAVAGLLL 1028

Query: 1024 ILSFWRIRQKRRRRRRGSGS-ARFPNL 1043
            +LSFWRIRQKRRRRRRGSGS ARF NL
Sbjct: 1029 LLSFWRIRQKRRRRRRGSGSAARFSNL 1053

BLAST of Sed0019002 vs. NCBI nr
Match: XP_022960716.1 (subtilisin-like protease SBT6.1 [Cucurbita moschata])

HSP 1 Score: 1932.5 bits (5005), Expect = 0.0e+00
Identity = 951/1047 (90.83%), Postives = 991/1047 (94.65%), Query Frame = 0

Query: 4    SSISFIII---IFISISFFQFKPSSDHTVFQTLARNHSPADPFPANNLTHGNRGSRNKNY 63
            SSISF +I   IFISIS FQF PSSDHTVFQTL RN+SP DPF + N+T GNR S+ KNY
Sbjct: 9    SSISFFVIFVPIFISISVFQFNPSSDHTVFQTLTRNYSPNDPFTSKNVTDGNRVSQ-KNY 68

Query: 64   IVRFVQYREAKEHRFYLESRVRSGGWGWIERRNAASKYPTDFGLVSIEESVRRELMEEIE 123
            IVRFVQYREAKEHRFYLESR+RSGGW WIERRN ASKYPTDFGLVSIE+SV  EL+EEIE
Sbjct: 69   IVRFVQYREAKEHRFYLESRIRSGGWKWIERRNPASKYPTDFGLVSIEDSVVMELVEEIE 128

Query: 124  ELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAAISNA 183
            ELELVKDVNVDASH RGL+ E GGR+GAFVDGKKRPGKIF+SMSFNEEGGERY+ AISNA
Sbjct: 129  ELELVKDVNVDASHVRGLLAEGGGRIGAFVDGKKRPGKIFTSMSFNEEGGERYT-AISNA 188

Query: 184  SNRWGRHLSMQRSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHPHFKNIKERTNWT 243
            SNRWGRHL MQRSQVTSLFGADSLW+KGYTG KVKMAIFDTGIRA+HPHF+NIKERTNWT
Sbjct: 189  SNRWGRHLLMQRSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIKERTNWT 248

Query: 244  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 303
            NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI
Sbjct: 249  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 308

Query: 304  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 363
            ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG
Sbjct: 309  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 368

Query: 364  VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 423
            VGGIDYNDHIASFSSRGMTTWEMPHG GRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS
Sbjct: 369  VGGIDYNDHIASFSSRGMTTWEMPHGNGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 428

Query: 424  PVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQGAGRVNLLESYEI 483
            PVVAGVVCLLVSVIPESQRK ILNPASMKQALVEGAAKLAGPN+YEQGAGRV+LLESYEI
Sbjct: 429  PVVAGVVCLLVSVIPESQRKTILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEI 488

Query: 484  LKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGMGVIGYVESQPTW 543
            LKSYQPRASIFP VLDYTDCPY+WPFCRQPLYAGAMPIIFNATILNGMGVIGYVE QPTW
Sbjct: 489  LKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTW 548

Query: 544  HPLNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS-PSSGE 603
            +P ++EGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS PS GE
Sbjct: 549  NPSDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGE 608

Query: 604  KNRQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHL 663
            KNR+ STCVLQLKLKVVPTPPRSKR+LWDQFH+IKYPPGYIPRDSLDVRNDILDWHGDHL
Sbjct: 609  KNRRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHL 668

Query: 664  HTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 723
            HTNFHIMFNMLRDAGYYVE+LGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT
Sbjct: 669  HTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 728

Query: 724  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 783
            GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG
Sbjct: 729  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 788

Query: 784  DFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILTSSMNKAEFPILGLLE 843
            DFSIDGEQSRYASGTDIVRFP+GGYVHSFPF+DSSESG AQSILTSS++KA+FPILGLLE
Sbjct: 789  DFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQSILTSSLSKADFPILGLLE 848

Query: 844  AVEGRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDPVLFTKSSKRNSPLYLEDN 903
            A EGRIAVYGDSNCLDSSHMVTNCYWLL+KILDFTSGNIRDPVLFTK SK+NSPLYLEDN
Sbjct: 849  AAEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKQNSPLYLEDN 908

Query: 904  KLPSRRSDVNFSTYSAVVGKELICRSDSRFEVWGTKGYSIHVRGRNRRLPGFPVFDLGRG 963
            KLPSRRSD NFS YSAV GKELIC SDSRFEVWGTKGYS+  RGRNRRLPGFP+ DLGR 
Sbjct: 909  KLPSRRSDANFSLYSAVSGKELICMSDSRFEVWGTKGYSVQGRGRNRRLPGFPLIDLGRD 968

Query: 964  LN---YSTSTRPPKLTLKDRTDTYANRYLSLFYRDEPGAPLIVPNHWLVPAIVALTGLLL 1023
            LN    S+   PPKLTLKDR+D Y NRYLSLFYRDEP  PLIVPNHWLVPA+VA+ GLLL
Sbjct: 969  LNSTPESSLMGPPKLTLKDRSDKYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVAVAGLLL 1028

Query: 1024 ILSFWRIRQKRRRRRRGSGS-ARFPNL 1043
            +LSFWRIRQKRRRRRRGSGS ARF NL
Sbjct: 1029 LLSFWRIRQKRRRRRRGSGSAARFSNL 1053

BLAST of Sed0019002 vs. NCBI nr
Match: XP_022987592.1 (subtilisin-like protease SBT6.1 [Cucurbita maxima])

HSP 1 Score: 1927.5 bits (4992), Expect = 0.0e+00
Identity = 950/1047 (90.74%), Postives = 990/1047 (94.56%), Query Frame = 0

Query: 4    SSISFIII---IFISISFFQFKPSSDHTVFQTLARNHSPADPFPANNLTHGNRGSRNKNY 63
            SSISF +I   IFISIS FQF PSSDHTVFQTL RN+SP DPF + N+T GNR S+ KNY
Sbjct: 9    SSISFFVIFVPIFISISVFQFNPSSDHTVFQTLTRNYSPNDPFTSKNVTDGNRVSQ-KNY 68

Query: 64   IVRFVQYREAKEHRFYLESRVRSGGWGWIERRNAASKYPTDFGLVSIEESVRRELMEEIE 123
            IVRFVQYREAKEHRFYLESR+RSGGW WIERRN ASKYPTDFGLVSIE+SV REL+EEIE
Sbjct: 69   IVRFVQYREAKEHRFYLESRIRSGGWKWIERRNPASKYPTDFGLVSIEDSVVRELVEEIE 128

Query: 124  ELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAAISNA 183
            E ELVKDVNVDASH RGL+ E GGR+GAFVDGKKRPGKIF+SMSFNE GGERY+ AISNA
Sbjct: 129  EFELVKDVNVDASHVRGLLAEGGGRIGAFVDGKKRPGKIFTSMSFNEGGGERYT-AISNA 188

Query: 184  SNRWGRHLSMQRSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHPHFKNIKERTNWT 243
            SNRWGRHLSMQRSQVTSLFGADSLW+KGYTG KVKMAIFDTGIRA+HPHF+NIKERTNWT
Sbjct: 189  SNRWGRHLSMQRSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIKERTNWT 248

Query: 244  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 303
            NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI
Sbjct: 249  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 308

Query: 304  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 363
            ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG
Sbjct: 309  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 368

Query: 364  VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 423
            VGGIDYNDHIASFSSRGMTTWEMPHG GRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS
Sbjct: 369  VGGIDYNDHIASFSSRGMTTWEMPHGNGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 428

Query: 424  PVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQGAGRVNLLESYEI 483
            PVVAGVVCLLVSVIPESQRK ILNPASMKQALVEGAAKLAGPN+YEQGAGRV+LLESYEI
Sbjct: 429  PVVAGVVCLLVSVIPESQRKTILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEI 488

Query: 484  LKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGMGVIGYVESQPTW 543
            LKSYQPRASIFP VLDYTDCPY+WPFCRQPLYAGAMPIIFNATILNGMGVIGYVE QPTW
Sbjct: 489  LKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTW 548

Query: 544  HPLNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS-PSSGE 603
            +P ++EGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS PS GE
Sbjct: 549  NPSDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGE 608

Query: 604  KNRQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHL 663
            KNR+ STCVLQLKLKVV TPPRSKR+LWDQFH+IKYPPGYIPRDSLDVRNDILDWHGDHL
Sbjct: 609  KNRRISTCVLQLKLKVVSTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHL 668

Query: 664  HTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 723
            HTNFHIMFNMLRDAGYYVE+LGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT
Sbjct: 669  HTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 728

Query: 724  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 783
            GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG
Sbjct: 729  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 788

Query: 784  DFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILTSSMNKAEFPILGLLE 843
            DFSIDGEQSRYASGTDIVRFP+ GYVHSFPF+DSSESG AQSILTSS++KA+FPILGLLE
Sbjct: 789  DFSIDGEQSRYASGTDIVRFPRDGYVHSFPFIDSSESGAAQSILTSSLSKADFPILGLLE 848

Query: 844  AVEGRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDPVLFTKSSKRNSPLYLEDN 903
            A EGRIAVYGDSNCLDSSHMVTNCYWLL+KILDFTSGNIRDPVLFTK SK+NSPLYLEDN
Sbjct: 849  AGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKQNSPLYLEDN 908

Query: 904  KLPSRRSDVNFSTYSAVVGKELICRSDSRFEVWGTKGYSIHVRGRNRRLPGFPVFDLGRG 963
            KLPSRRSDVNFS YSAV GKELIC SDSRFEVWGTKGYS+  RGRNRRLPGFP+ DLGR 
Sbjct: 909  KLPSRRSDVNFSLYSAVSGKELICMSDSRFEVWGTKGYSVQGRGRNRRLPGFPLIDLGRD 968

Query: 964  LN---YSTSTRPPKLTLKDRTDTYANRYLSLFYRDEPGAPLIVPNHWLVPAIVALTGLLL 1023
            LN    S+   PPKLTLKDR+D Y NRYLSLFYRDEP  PLIVPNHWLVPA+VA+ GLLL
Sbjct: 969  LNSTPESSLMGPPKLTLKDRSDKYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVAVAGLLL 1028

Query: 1024 ILSFWRIRQKRRRRRRGSGSA-RFPNL 1043
            +LSFWRIRQKRRRRRRGSGSA RF NL
Sbjct: 1029 LLSFWRIRQKRRRRRRGSGSAPRFSNL 1053

BLAST of Sed0019002 vs. ExPASy Swiss-Prot
Match: Q0WUG6 (Subtilisin-like protease SBT6.1 OS=Arabidopsis thaliana OX=3702 GN=SBT6.1 PE=1 SV=1)

HSP 1 Score: 1602.0 bits (4147), Expect = 0.0e+00
Identity = 778/1038 (74.95%), Postives = 885/1038 (85.26%), Query Frame = 0

Query: 7    SFIIIIFISISFFQFKPSSDHTVFQTLARNHSPADPFPANNLTHGNRGSRNKNYIVRFVQ 66
            S II++F+S+S F  +PS+ H   Q L          P N     +      NYI+RF Q
Sbjct: 14   SCIIVVFLSVSLFWLRPSTYHPQQQNLN---------PENVTRLESENETKTNYIIRFKQ 73

Query: 67   YREAKEHRFYLESRVRSGGWGWIERRNAASKYPTDFGLVSIEESVRRELMEEIEELELVK 126
            Y+ AK+HR YLES+VRSGGWGWIER N A+KYPTDFG++ IEES +  ++ EIE LE+VK
Sbjct: 74   YKPAKDHRIYLESKVRSGGWGWIERINPATKYPTDFGVLWIEESGKEAVVGEIERLEMVK 133

Query: 127  DVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAAISNASNRWGR 186
            DVNV+  + R L+       G+F DGKKRPGKIF+SMSF E       A  SN +  W R
Sbjct: 134  DVNVEFKYQRVLLG------GSFPDGKKRPGKIFTSMSFEEGTESSPMADTSNTTLNWSR 193

Query: 187  HLSMQRSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHPHFKNIKERTNWTNEDTLN 246
            HL  Q++QVTS+FGAD LW KGYTG KVKMAIFDTGIRA HPHF+ IKERTNWTNEDTLN
Sbjct: 194  HLLAQKTQVTSMFGADHLWKKGYTGAKVKMAIFDTGIRADHPHFRKIKERTNWTNEDTLN 253

Query: 247  DNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAIATNMDV 306
            DNLGHGTFVAGVIAG + ECLGFA DTEIYAFRVFTDAQVSYTSWFLDAFNYAIAT+MDV
Sbjct: 254  DNLGHGTFVAGVIAGRNPECLGFASDTEIYAFRVFTDAQVSYTSWFLDAFNYAIATDMDV 313

Query: 307  LNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGGIDY 366
            LNLSIGGPDYLDLPFVEK+WEITA+NIIMVSAIGNDGPLYGTLNNPADQSDVIGVGGID 
Sbjct: 314  LNLSIGGPDYLDLPFVEKVWEITASNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGGIDN 373

Query: 367  NDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVVAGV 426
            +DHIASFSSRGM+TWE+PHGYGRVKPDVVAYGR+IMGSKISTGCKSLSGTSVASPVVAG+
Sbjct: 374  DDHIASFSSRGMSTWELPHGYGRVKPDVVAYGRDIMGSKISTGCKSLSGTSVASPVVAGI 433

Query: 427  VCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQGAGRVNLLESYEILKSYQP 486
            VCLLVSVIPE++RK +LNPASMKQALVEGAAKL+GPN+YEQGAGRV+LLESYEILKSY P
Sbjct: 434  VCLLVSVIPEARRKDLLNPASMKQALVEGAAKLSGPNMYEQGAGRVDLLESYEILKSYHP 493

Query: 487  RASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGMGVIGYVESQPTWHPLNDE 546
            RASIFPS+LDY DCPYSWPFCRQPLYAGAMPIIFN TILNGMGVIGY+ES PTWHP N+E
Sbjct: 494  RASIFPSILDYNDCPYSWPFCRQPLYAGAMPIIFNTTILNGMGVIGYIESPPTWHPANEE 553

Query: 547  GNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS-PSSGEKNRQTS 606
            GNLLSIHF Y  VIWPWTGY+ALHMQIKEEGAQF+GEIEGNVT+ VYS P+SGE   + S
Sbjct: 554  GNLLSIHFKYPDVIWPWTGYLALHMQIKEEGAQFTGEIEGNVTVKVYSPPASGESGPRRS 613

Query: 607  TCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHLHTNFHI 666
            TC LQLKLKV+PTPPR+KR+LWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHLHTNFHI
Sbjct: 614  TCSLQLKLKVIPTPPRAKRILWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHLHTNFHI 673

Query: 667  MFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMITGLGLAV 726
            M+NMLRDAGYY+E+LGSPLTCFDA+QYGTLL+VDLED+YF EEIEKLRDDV+ TGLGL V
Sbjct: 674  MYNMLRDAGYYIETLGSPLTCFDAQQYGTLLMVDLEDDYFPEEIEKLRDDVINTGLGLVV 733

Query: 727  FSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFSIDG 786
            F+EWYNV+TMVKMRFFDDNTRSWWTPVTGGANIPALN+LLA FGIAFGDKILNGDFSIDG
Sbjct: 734  FAEWYNVDTMVKMRFFDDNTRSWWTPVTGGANIPALNNLLASFGIAFGDKILNGDFSIDG 793

Query: 787  EQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILTSSMNKAEFPILGLLEAVEGRI 846
            EQSRYASGT+IVRFP GG++H+FP +DSSESG  Q++L +  +K +  +LGLLE  EGR+
Sbjct: 794  EQSRYASGTNIVRFPAGGFLHTFPLLDSSESGATQNLLLTEASKEDPAVLGLLEIGEGRV 853

Query: 847  AVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDPVLFTKSSKRNSPLYLEDNKLPSRR 906
             VYGDSNCLDSSHMVTNCYWLLKK+LDF+S NI+DPVLF+K +KR SP+ +++ +LPSRR
Sbjct: 854  GVYGDSNCLDSSHMVTNCYWLLKKMLDFSSSNIKDPVLFSKFAKRYSPVIIDEKQLPSRR 913

Query: 907  SDVNFSTYSAVVGKELICRSDSRFEVWGTKGYSIHVRGRNRRLPGFPVFDLGRGLNYSTS 966
            +DVNFSTYS+V+GKELIC SDSRFEVWGTKGY++HVRGRNRRLPG+   DLGRGLN++  
Sbjct: 914  TDVNFSTYSSVIGKELICESDSRFEVWGTKGYNLHVRGRNRRLPGYHGIDLGRGLNFTVE 973

Query: 967  TRPPK--LTLKDRTDTYANRYLS---LFYRDEPGAPLIVPNHWLVPAIVALTGLLLILSF 1026
            ++ P    + K+  +  ++R  S   LF RDE   P +VP  W+V A V  +G+L++LS 
Sbjct: 974  SKRPTRWRSAKEGGELSSSRSKSLGGLFNRDEIDMPFLVPTRWIVLAGVVASGVLVLLSI 1033

Query: 1027 WRIRQKRRRRRRGSGSAR 1039
            WRIRQKR RRRR SGS R
Sbjct: 1034 WRIRQKRGRRRRASGSNR 1036

BLAST of Sed0019002 vs. ExPASy Swiss-Prot
Match: Q14703 (Membrane-bound transcription factor site-1 protease OS=Homo sapiens OX=9606 GN=MBTPS1 PE=1 SV=1)

HSP 1 Score: 881.3 bits (2276), Expect = 1.1e-254
Identity = 450/880 (51.14%), Postives = 583/880 (66.25%), Query Frame = 0

Query: 60  YIVRFVQYREAKEHRFYLESRVRSG---GWGWIERRNAASKYPTDFGLVSIEESVRRELM 119
           YIV F  Y  AK    ++ S ++S     W  I R N +S YP+DF ++ I+E  +  L+
Sbjct: 56  YIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLL 115

Query: 120 EEIEELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAA 179
             +E+   +K V       R L   +        D      +   S  +      R  A+
Sbjct: 116 -TLEDHPNIKRVTPQRKVFRSLKYAES-------DPTVPCNETRWSQKWQSSRPLR-RAS 175

Query: 180 ISNASNRW---GRHLSMQ-----RSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHP 239
           +S  S  W   GRH S +       QV     AD LW  GYTG  V++A+FDTG+   HP
Sbjct: 176 LSLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHP 235

Query: 240 HFKNIKERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSY 299
           HFKN+KERTNWTNE TL+D LGHGTFVAGVIA    EC GFAPD E++ FRVFT+ QVSY
Sbjct: 236 HFKNVKERTNWTNERTLDDGLGHGTFVAGVIA-SMRECQGFAPDAELHIFRVFTNNQVSY 295

Query: 300 TSWFLDAFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGT 359
           TSWFLDAFNYAI   +DVLNLSIGGPD++D PFV+K+WE+TANN+IMVSAIGNDGPLYGT
Sbjct: 296 TSWFLDAFNYAILKKIDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGT 355

Query: 360 LNNPADQSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKIST 419
           LNNPADQ DVIGVGGID+ D+IA FSSRGMTTWE+P GYGR+KPD+V YG  + GS +  
Sbjct: 356 LNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRMKPDIVTYGAGVRGSGVKG 415

Query: 420 GCKSLSGTSVASPVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQG 479
           GC++LSGTSVASPVVAG V LLVS +   Q++ ++NPASMKQAL+  A +L G N++EQG
Sbjct: 416 GCRALSGTSVASPVVAGAVTLLVSTV---QKRELVNPASMKQALIASARRLPGVNMFEQG 475

Query: 480 AGRVNLLESYEILKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGM 539
            G+++LL +Y+IL SY+P+AS+ PS +D T+CPY WP+C QP+Y G MP + N TILNGM
Sbjct: 476 HGKLDLLRAYQILNSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTVVNVTILNGM 535

Query: 540 GVIGYVESQPTWHP-LNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGN 599
           GV G +  +P W P L   G+ + + F+YS V+WPW+GY+A+ + + ++ A + G  +G+
Sbjct: 536 GVTGRIVDKPDWQPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGH 595

Query: 600 VTLTVYSPSSGE-KN--RQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDS 659
           V +TV SP+  E KN   QTST  L +K+K++PTPPRSKRVLWDQ+H+++YPPGY PRD+
Sbjct: 596 VMITVASPAETESKNGAEQTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDN 655

Query: 660 LDVRNDILDWHGDHLHTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEY 719
           L ++ND LDW+GDH+HTNF  M+  LR  GY+VE LG+P TCFDA QYGTLL+VD E+EY
Sbjct: 656 LRMKNDPLDWNGDHIHTNFRDMYQHLRSMGYFVEVLGAPFTCFDASQYGTLLMVDSEEEY 715

Query: 720 FKEEIEKLRDDVMITGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDL 779
           F EEI KLR DV   GL L +FS+WYN   M K++F+D+NTR WW P TGGANIPALN+L
Sbjct: 716 FPEEIAKLRRDV-DNGLSLVIFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANIPALNEL 775

Query: 780 LAPFGIAFGDKILNGDFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILT 839
           L+ + + F D +  G+F++      YASG  I +FP+ G V +  F D     + Q    
Sbjct: 776 LSVWNMGFSDGLYEGEFTLANHDMYYASGCSIAKFPEDGVVITQTFKDQGLEVLKQETAV 835

Query: 840 SSMNKAEFPILGLLEAVE---GRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDP 899
                   PILGL +      GRI +YGDSNCLD SH   +C+WLL  +L +TS  +  P
Sbjct: 836 VE----NVPILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPP 895

Query: 900 VLFTKSSKRNSPLYLEDNKLPSRRSDVNFSTYSAVVGKEL 922
            L + S  R  P     +  P R    +   YS V+   L
Sbjct: 896 SL-SHSGNRQRPPSGAGSVTPERMEGNHLHRYSKVLEAHL 916

BLAST of Sed0019002 vs. ExPASy Swiss-Prot
Match: Q9WTZ2 (Membrane-bound transcription factor site-1 protease OS=Mus musculus OX=10090 GN=Mbtps1 PE=1 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 2.3e-254
Identity = 449/880 (51.02%), Postives = 580/880 (65.91%), Query Frame = 0

Query: 60  YIVRFVQYREAKEHRFYLESRVRSG---GWGWIERRNAASKYPTDFGLVSIEESVRRELM 119
           YIV F  Y  AK    ++ S ++S     W  I R N +S YP+DF ++ I+E  +  L+
Sbjct: 56  YIVAFNGYFTAKARNSFISSALKSSEVENWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLL 115

Query: 120 EEIEELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAA 179
             +E+   +K V       R L   +   +    +  +   K  SS            A+
Sbjct: 116 -TLEDHPNIKRVTPQRKVFRSLKFAESNPI-VPCNETRWSQKWQSSRPLKR-------AS 175

Query: 180 ISNASNRW---GRHLSMQ-----RSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHP 239
           +S  S  W   GRH S +       QV     AD LW  GYTG  V++A+FDTG+   HP
Sbjct: 176 LSLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHP 235

Query: 240 HFKNIKERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSY 299
           HFKN+KERTNWTNE TL+D LGHGTFVAGVIA    EC GFAPD E++ FRVFT+ QVSY
Sbjct: 236 HFKNVKERTNWTNERTLDDGLGHGTFVAGVIA-SMRECQGFAPDAELHIFRVFTNNQVSY 295

Query: 300 TSWFLDAFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGT 359
           TSWFLDAFNYAI   MDVLNLSIGGPD++D PFV+K+WE+TANN+IMVSAIGNDGPLYGT
Sbjct: 296 TSWFLDAFNYAILKKMDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGT 355

Query: 360 LNNPADQSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKIST 419
           LNNPADQ DVIGVGGID+ D+IA FSSRGMTTWE+P GYGRVKPD+V YG  + GS +  
Sbjct: 356 LNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKG 415

Query: 420 GCKSLSGTSVASPVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQG 479
           GC++LSGTSVASPVVAG V LLVS +   Q++ ++NPAS+KQAL+  A +L G N++EQG
Sbjct: 416 GCRALSGTSVASPVVAGAVTLLVSTV---QKRELVNPASVKQALIASARRLPGVNMFEQG 475

Query: 480 AGRVNLLESYEILKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGM 539
            G+++LL +Y+IL SY+P+AS+ PS +D T+CPY WP+C QP+Y G MP I N TILNGM
Sbjct: 476 HGKLDLLRAYQILSSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGM 535

Query: 540 GVIGYVESQPTWHP-LNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGN 599
           GV G +  +P W P L   G+ + + F+YS V+WPW+GY+A+ + + ++ A + G  +G+
Sbjct: 536 GVTGRIVDKPEWRPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGH 595

Query: 600 VTLTVYSPSSGEKN---RQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDS 659
           + +TV SP+  E +     TST  L +K+K++PTPPRSKRVLWDQ+H+++YPPGY PRD+
Sbjct: 596 IMITVASPAETELHSGAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDN 655

Query: 660 LDVRNDILDWHGDHLHTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEY 719
           L ++ND LDW+GDH+HTNF  M+  LR  GY+VE LG+P TCFDA QYGTLLLVD E+EY
Sbjct: 656 LRMKNDPLDWNGDHVHTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLLVDSEEEY 715

Query: 720 FKEEIEKLRDDVMITGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDL 779
           F EEI KLR DV   GL L +FS+WYN   M K++F+D+NTR WW P TGGANIPALN+L
Sbjct: 716 FPEEIAKLRRDV-DNGLSLVIFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANIPALNEL 775

Query: 780 LAPFGIAFGDKILNGDFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILT 839
           L+ + + F D +  G+F +      YASG  I +FP+ G V +  F D     + Q    
Sbjct: 776 LSVWNMGFSDGLYEGEFVLANHDMYYASGCSIAKFPEDGVVITQTFKDQGLEVLKQETAV 835

Query: 840 SSMNKAEFPILGLLEAVE---GRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDP 899
                   PILGL +      GRI +YGDSNCLD SH   +C+WLL  +L +TS  +  P
Sbjct: 836 VE----NVPILGLYQIPSEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPP 895

Query: 900 VLFTKSSKRNSPLYLEDNKLPSRRSDVNFSTYSAVVGKEL 922
            L + S  R  P        P R    +   YS V+   L
Sbjct: 896 SL-SHSGNRQRPPSGAGLAPPERMEGNHLHRYSKVLEAHL 916

BLAST of Sed0019002 vs. ExPASy Swiss-Prot
Match: Q9WTZ3 (Membrane-bound transcription factor site-1 protease OS=Rattus norvegicus OX=10116 GN=Mbtps1 PE=1 SV=2)

HSP 1 Score: 879.8 bits (2272), Expect = 3.1e-254
Identity = 450/880 (51.14%), Postives = 582/880 (66.14%), Query Frame = 0

Query: 60  YIVRFVQYREAKEHRFYLESRVRSG---GWGWIERRNAASKYPTDFGLVSIEESVRRELM 119
           YIV F  Y  AK    ++ S ++S     W  I R N +S YP+DF ++ I+E  +  L+
Sbjct: 56  YIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLL 115

Query: 120 EEIEELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAA 179
             +E+   +K V       R L   +   +    +  +   K  SS            A+
Sbjct: 116 -TLEDHPNIKRVTPQRKVFRSLKFAESDPI-VPCNETRWSQKWQSSRPLKR-------AS 175

Query: 180 ISNASNRW---GRHLSMQ-----RSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHP 239
           +S  S  W   GRH S +       QV     AD LW  GYTG  V++A+FDTG+   HP
Sbjct: 176 LSLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHP 235

Query: 240 HFKNIKERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSY 299
           HFKN+KERTNWTNE TL+D LGHGTFVAGVIA    EC GFAPD E++ FRVFT+ QVSY
Sbjct: 236 HFKNVKERTNWTNERTLDDGLGHGTFVAGVIA-SMRECQGFAPDAELHIFRVFTNNQVSY 295

Query: 300 TSWFLDAFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGT 359
           TSWFLDAFNYAI   +DVLNLSIGGPD++D PFV+K+WE+TANN+IMVSAIGNDGPLYGT
Sbjct: 296 TSWFLDAFNYAILKKIDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGT 355

Query: 360 LNNPADQSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKIST 419
           LNNPADQ DVIGVGGID+ D+IA FSSRGMTTWE+P GYGRVKPD+V YG  + GS +  
Sbjct: 356 LNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKG 415

Query: 420 GCKSLSGTSVASPVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQG 479
           GC++LSGTSVASPVVAG V LLVS +   Q++ ++NPAS+KQAL+  A +L G N++EQG
Sbjct: 416 GCRALSGTSVASPVVAGAVTLLVSTV---QKRELVNPASVKQALIASARRLPGVNMFEQG 475

Query: 480 AGRVNLLESYEILKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGM 539
            G+++LL +Y+IL SY+P+AS+ PS +D T+CPY WP+C QP+Y G MP I N TILNGM
Sbjct: 476 HGKLDLLRAYQILSSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGM 535

Query: 540 GVIGYVESQPTWHP-LNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGN 599
           GV G +  +P W P L   G+ + + F+YS V+WPW+GY+A+ + + ++ A + G  +G+
Sbjct: 536 GVTGRIVDKPEWRPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGH 595

Query: 600 VTLTVYSPSSGE-KN--RQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDS 659
           + +TV SP+  E KN    TST  L +K+K++PTPPRSKRVLWDQ+H+++YPPGY PRD+
Sbjct: 596 IMITVASPAETELKNGAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDN 655

Query: 660 LDVRNDILDWHGDHLHTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEY 719
           L ++ND LDW+GDH+HTNF  M+  LR  GY+VE LG+P TCFDA QYGTLL+VD E+EY
Sbjct: 656 LRMKNDPLDWNGDHVHTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLMVDSEEEY 715

Query: 720 FKEEIEKLRDDVMITGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDL 779
           F EEI KLR DV   GL L VFS+WYN   M K++F+D+NTR WW P TGGAN+PALN+L
Sbjct: 716 FPEEIAKLRRDV-DNGLSLVVFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANVPALNEL 775

Query: 780 LAPFGIAFGDKILNGDFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILT 839
           L+ + + F D +  G+F++      YASG  I RFP+ G V +  F D     + Q    
Sbjct: 776 LSVWNMGFSDGLYEGEFALANHDMYYASGCSIARFPEDGVVITQTFKDQGLEVLKQETAV 835

Query: 840 SSMNKAEFPILGLLEAVE---GRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDP 899
                   PILGL +      GRI +YGDSNCLD SH   +C+WLL  +L +TS  +  P
Sbjct: 836 VD----NVPILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPP 895

Query: 900 VLFTKSSKRNSPLYLEDNKLPSRRSDVNFSTYSAVVGKEL 922
            L + S  R  P        P R    +   YS V+   L
Sbjct: 896 SL-SHSGNRQRPPSGAGLAPPERMEGNHLHRYSKVLEAHL 916

BLAST of Sed0019002 vs. ExPASy Swiss-Prot
Match: Q9Z2A8 (Membrane-bound transcription factor site-1 protease OS=Cricetulus griseus OX=10029 GN=MBTPS1 PE=1 SV=2)

HSP 1 Score: 878.6 bits (2269), Expect = 6.8e-254
Identity = 449/880 (51.02%), Postives = 582/880 (66.14%), Query Frame = 0

Query: 60  YIVRFVQYREAKEHRFYLESRVRSG---GWGWIERRNAASKYPTDFGLVSIEESVRRELM 119
           YIV F  Y  AK    ++ S ++S     W  I R N +S YP+DF ++ I+E  +  L+
Sbjct: 56  YIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLL 115

Query: 120 EEIEELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAA 179
             +E+   +K V       R L   +   +    +  +   K  SS            A+
Sbjct: 116 -TLEDHPNIKRVTPQRKVFRSLKFAESDPI-VPCNETRWSQKWQSSRPLRR-------AS 175

Query: 180 ISNASNRW---GRHLSMQ-----RSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHP 239
           +S  S  W   GRH S +       QV     AD LW  GYTG  V++A+FDTG+   HP
Sbjct: 176 LSLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHP 235

Query: 240 HFKNIKERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSY 299
           HFKN+KERTNWTNE TL+D LGHGTFVAGVIA    EC GFAPD E++ FRVFT+ QVSY
Sbjct: 236 HFKNVKERTNWTNERTLDDGLGHGTFVAGVIA-SMRECQGFAPDAELHIFRVFTNNQVSY 295

Query: 300 TSWFLDAFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGT 359
           TSWFLDAFNYAI   +DVLNLSIGGPD++D PFV+K+WE+TANN+IMVSAIGNDGPLYGT
Sbjct: 296 TSWFLDAFNYAILKKIDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGT 355

Query: 360 LNNPADQSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKIST 419
           LNNPADQ DVIGVGGID+ D+IA FSSRGMTTWE+P GYGRVKPD+V YG  + GS +  
Sbjct: 356 LNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKG 415

Query: 420 GCKSLSGTSVASPVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQG 479
           GC++LSGTSVASPVVAG V LLVS +   Q++ ++NPAS+KQAL+  A +L G N++EQG
Sbjct: 416 GCRALSGTSVASPVVAGAVTLLVSTV---QKRELVNPASVKQALIASARRLPGVNMFEQG 475

Query: 480 AGRVNLLESYEILKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGM 539
            G+++LL +Y+IL SY+P+AS+ PS +D T+CPY WP+C QP+Y G MP I N TILNGM
Sbjct: 476 HGKLDLLRAYQILSSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGM 535

Query: 540 GVIGYVESQPTWHP-LNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGN 599
           GV G +  +P W P L   G+ + + F+YS V+WPW+GY+A+ + + ++ A + G  +G+
Sbjct: 536 GVTGRIVDKPEWRPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGH 595

Query: 600 VTLTVYSPSSGE-KN--RQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDS 659
           + +TV SP+  E KN    TST  L +K+K++PTPPRSKRVLWDQ+H+++YPPGY PRD+
Sbjct: 596 IMITVASPAETEAKNGAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDN 655

Query: 660 LDVRNDILDWHGDHLHTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEY 719
           L ++ND LDW+GDH+HTNF  M+  LR  GY+VE LG+P TCFDA QYGTLL+VD E+EY
Sbjct: 656 LRMKNDPLDWNGDHVHTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLMVDSEEEY 715

Query: 720 FKEEIEKLRDDVMITGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDL 779
           F EEI KLR DV   GL L +FS+WYN   M K++F+D+NTR WW P TGGANIPALN+L
Sbjct: 716 FPEEIAKLRRDV-DNGLSLVIFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANIPALNEL 775

Query: 780 LAPFGIAFGDKILNGDFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILT 839
           L+ + + F D +  G+F++      YASG  I +FP+ G V +  F D     + Q    
Sbjct: 776 LSVWNMGFSDGLYEGEFALANHDMYYASGCSIAKFPEDGVVITQTFKDQGLEVLKQETAV 835

Query: 840 SSMNKAEFPILGLLEAVE---GRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDP 899
                   PILGL +      GRI +YGDSNCLD SH   +C+WLL  +L +TS  +  P
Sbjct: 836 VE----NVPILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPP 895

Query: 900 VLFTKSSKRNSPLYLEDNKLPSRRSDVNFSTYSAVVGKEL 922
            L + S  R  P        P R    +   YS V+   L
Sbjct: 896 SL-SHSGNRQRPPSGAGLAPPERMEGNHLHRYSKVLEAHL 916

BLAST of Sed0019002 vs. ExPASy TrEMBL
Match: A0A6J1H8D8 (subtilisin-like protease SBT6.1 OS=Cucurbita moschata OX=3662 GN=LOC111461434 PE=3 SV=1)

HSP 1 Score: 1932.5 bits (5005), Expect = 0.0e+00
Identity = 951/1047 (90.83%), Postives = 991/1047 (94.65%), Query Frame = 0

Query: 4    SSISFIII---IFISISFFQFKPSSDHTVFQTLARNHSPADPFPANNLTHGNRGSRNKNY 63
            SSISF +I   IFISIS FQF PSSDHTVFQTL RN+SP DPF + N+T GNR S+ KNY
Sbjct: 9    SSISFFVIFVPIFISISVFQFNPSSDHTVFQTLTRNYSPNDPFTSKNVTDGNRVSQ-KNY 68

Query: 64   IVRFVQYREAKEHRFYLESRVRSGGWGWIERRNAASKYPTDFGLVSIEESVRRELMEEIE 123
            IVRFVQYREAKEHRFYLESR+RSGGW WIERRN ASKYPTDFGLVSIE+SV  EL+EEIE
Sbjct: 69   IVRFVQYREAKEHRFYLESRIRSGGWKWIERRNPASKYPTDFGLVSIEDSVVMELVEEIE 128

Query: 124  ELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAAISNA 183
            ELELVKDVNVDASH RGL+ E GGR+GAFVDGKKRPGKIF+SMSFNEEGGERY+ AISNA
Sbjct: 129  ELELVKDVNVDASHVRGLLAEGGGRIGAFVDGKKRPGKIFTSMSFNEEGGERYT-AISNA 188

Query: 184  SNRWGRHLSMQRSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHPHFKNIKERTNWT 243
            SNRWGRHL MQRSQVTSLFGADSLW+KGYTG KVKMAIFDTGIRA+HPHF+NIKERTNWT
Sbjct: 189  SNRWGRHLLMQRSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIKERTNWT 248

Query: 244  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 303
            NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI
Sbjct: 249  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 308

Query: 304  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 363
            ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG
Sbjct: 309  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 368

Query: 364  VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 423
            VGGIDYNDHIASFSSRGMTTWEMPHG GRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS
Sbjct: 369  VGGIDYNDHIASFSSRGMTTWEMPHGNGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 428

Query: 424  PVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQGAGRVNLLESYEI 483
            PVVAGVVCLLVSVIPESQRK ILNPASMKQALVEGAAKLAGPN+YEQGAGRV+LLESYEI
Sbjct: 429  PVVAGVVCLLVSVIPESQRKTILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEI 488

Query: 484  LKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGMGVIGYVESQPTW 543
            LKSYQPRASIFP VLDYTDCPY+WPFCRQPLYAGAMPIIFNATILNGMGVIGYVE QPTW
Sbjct: 489  LKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTW 548

Query: 544  HPLNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS-PSSGE 603
            +P ++EGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS PS GE
Sbjct: 549  NPSDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGE 608

Query: 604  KNRQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHL 663
            KNR+ STCVLQLKLKVVPTPPRSKR+LWDQFH+IKYPPGYIPRDSLDVRNDILDWHGDHL
Sbjct: 609  KNRRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHL 668

Query: 664  HTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 723
            HTNFHIMFNMLRDAGYYVE+LGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT
Sbjct: 669  HTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 728

Query: 724  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 783
            GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG
Sbjct: 729  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 788

Query: 784  DFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILTSSMNKAEFPILGLLE 843
            DFSIDGEQSRYASGTDIVRFP+GGYVHSFPF+DSSESG AQSILTSS++KA+FPILGLLE
Sbjct: 789  DFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQSILTSSLSKADFPILGLLE 848

Query: 844  AVEGRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDPVLFTKSSKRNSPLYLEDN 903
            A EGRIAVYGDSNCLDSSHMVTNCYWLL+KILDFTSGNIRDPVLFTK SK+NSPLYLEDN
Sbjct: 849  AAEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKQNSPLYLEDN 908

Query: 904  KLPSRRSDVNFSTYSAVVGKELICRSDSRFEVWGTKGYSIHVRGRNRRLPGFPVFDLGRG 963
            KLPSRRSD NFS YSAV GKELIC SDSRFEVWGTKGYS+  RGRNRRLPGFP+ DLGR 
Sbjct: 909  KLPSRRSDANFSLYSAVSGKELICMSDSRFEVWGTKGYSVQGRGRNRRLPGFPLIDLGRD 968

Query: 964  LN---YSTSTRPPKLTLKDRTDTYANRYLSLFYRDEPGAPLIVPNHWLVPAIVALTGLLL 1023
            LN    S+   PPKLTLKDR+D Y NRYLSLFYRDEP  PLIVPNHWLVPA+VA+ GLLL
Sbjct: 969  LNSTPESSLMGPPKLTLKDRSDKYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVAVAGLLL 1028

Query: 1024 ILSFWRIRQKRRRRRRGSGS-ARFPNL 1043
            +LSFWRIRQKRRRRRRGSGS ARF NL
Sbjct: 1029 LLSFWRIRQKRRRRRRGSGSAARFSNL 1053

BLAST of Sed0019002 vs. ExPASy TrEMBL
Match: A0A6J1JAS5 (subtilisin-like protease SBT6.1 OS=Cucurbita maxima OX=3661 GN=LOC111485106 PE=3 SV=1)

HSP 1 Score: 1927.5 bits (4992), Expect = 0.0e+00
Identity = 950/1047 (90.74%), Postives = 990/1047 (94.56%), Query Frame = 0

Query: 4    SSISFIII---IFISISFFQFKPSSDHTVFQTLARNHSPADPFPANNLTHGNRGSRNKNY 63
            SSISF +I   IFISIS FQF PSSDHTVFQTL RN+SP DPF + N+T GNR S+ KNY
Sbjct: 9    SSISFFVIFVPIFISISVFQFNPSSDHTVFQTLTRNYSPNDPFTSKNVTDGNRVSQ-KNY 68

Query: 64   IVRFVQYREAKEHRFYLESRVRSGGWGWIERRNAASKYPTDFGLVSIEESVRRELMEEIE 123
            IVRFVQYREAKEHRFYLESR+RSGGW WIERRN ASKYPTDFGLVSIE+SV REL+EEIE
Sbjct: 69   IVRFVQYREAKEHRFYLESRIRSGGWKWIERRNPASKYPTDFGLVSIEDSVVRELVEEIE 128

Query: 124  ELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAAISNA 183
            E ELVKDVNVDASH RGL+ E GGR+GAFVDGKKRPGKIF+SMSFNE GGERY+ AISNA
Sbjct: 129  EFELVKDVNVDASHVRGLLAEGGGRIGAFVDGKKRPGKIFTSMSFNEGGGERYT-AISNA 188

Query: 184  SNRWGRHLSMQRSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHPHFKNIKERTNWT 243
            SNRWGRHLSMQRSQVTSLFGADSLW+KGYTG KVKMAIFDTGIRA+HPHF+NIKERTNWT
Sbjct: 189  SNRWGRHLSMQRSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIKERTNWT 248

Query: 244  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 303
            NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI
Sbjct: 249  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 308

Query: 304  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 363
            ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG
Sbjct: 309  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 368

Query: 364  VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 423
            VGGIDYNDHIASFSSRGMTTWEMPHG GRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS
Sbjct: 369  VGGIDYNDHIASFSSRGMTTWEMPHGNGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 428

Query: 424  PVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQGAGRVNLLESYEI 483
            PVVAGVVCLLVSVIPESQRK ILNPASMKQALVEGAAKLAGPN+YEQGAGRV+LLESYEI
Sbjct: 429  PVVAGVVCLLVSVIPESQRKTILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEI 488

Query: 484  LKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGMGVIGYVESQPTW 543
            LKSYQPRASIFP VLDYTDCPY+WPFCRQPLYAGAMPIIFNATILNGMGVIGYVE QPTW
Sbjct: 489  LKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTW 548

Query: 544  HPLNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS-PSSGE 603
            +P ++EGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS PS GE
Sbjct: 549  NPSDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGE 608

Query: 604  KNRQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHL 663
            KNR+ STCVLQLKLKVV TPPRSKR+LWDQFH+IKYPPGYIPRDSLDVRNDILDWHGDHL
Sbjct: 609  KNRRISTCVLQLKLKVVSTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHL 668

Query: 664  HTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 723
            HTNFHIMFNMLRDAGYYVE+LGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT
Sbjct: 669  HTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 728

Query: 724  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 783
            GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG
Sbjct: 729  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 788

Query: 784  DFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILTSSMNKAEFPILGLLE 843
            DFSIDGEQSRYASGTDIVRFP+ GYVHSFPF+DSSESG AQSILTSS++KA+FPILGLLE
Sbjct: 789  DFSIDGEQSRYASGTDIVRFPRDGYVHSFPFIDSSESGAAQSILTSSLSKADFPILGLLE 848

Query: 844  AVEGRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDPVLFTKSSKRNSPLYLEDN 903
            A EGRIAVYGDSNCLDSSHMVTNCYWLL+KILDFTSGNIRDPVLFTK SK+NSPLYLEDN
Sbjct: 849  AGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKQNSPLYLEDN 908

Query: 904  KLPSRRSDVNFSTYSAVVGKELICRSDSRFEVWGTKGYSIHVRGRNRRLPGFPVFDLGRG 963
            KLPSRRSDVNFS YSAV GKELIC SDSRFEVWGTKGYS+  RGRNRRLPGFP+ DLGR 
Sbjct: 909  KLPSRRSDVNFSLYSAVSGKELICMSDSRFEVWGTKGYSVQGRGRNRRLPGFPLIDLGRD 968

Query: 964  LN---YSTSTRPPKLTLKDRTDTYANRYLSLFYRDEPGAPLIVPNHWLVPAIVALTGLLL 1023
            LN    S+   PPKLTLKDR+D Y NRYLSLFYRDEP  PLIVPNHWLVPA+VA+ GLLL
Sbjct: 969  LNSTPESSLMGPPKLTLKDRSDKYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVAVAGLLL 1028

Query: 1024 ILSFWRIRQKRRRRRRGSGSA-RFPNL 1043
            +LSFWRIRQKRRRRRRGSGSA RF NL
Sbjct: 1029 LLSFWRIRQKRRRRRRGSGSAPRFSNL 1053

BLAST of Sed0019002 vs. ExPASy TrEMBL
Match: A0A0A0LTP5 (Peptidase_S8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G250160 PE=3 SV=1)

HSP 1 Score: 1926.4 bits (4989), Expect = 0.0e+00
Identity = 945/1046 (90.34%), Postives = 987/1046 (94.36%), Query Frame = 0

Query: 4    SSISFIII---IFISISFFQFKPSSDHTVFQTLARNHSPADPFPANNLTHGNRGSRNKNY 63
            SSISFI I   IFISI  FQFKPSSDH    TL  N+S  DP P NNLTHGN+  R +NY
Sbjct: 5    SSISFIAIFLPIFISIYLFQFKPSSDH----TLTPNYSTFDPSPFNNLTHGNQVLRKQNY 64

Query: 64   IVRFVQYREAKEHRFYLESRVRSGGWGWIERRNAASKYPTDFGLVSIEESVRRELMEEIE 123
            IVRF+ YR+AK+HRFYLES VRSGGW WI+RRN ASKYPTDFGLVSIE+SVR EL+EEIE
Sbjct: 65   IVRFLHYRKAKDHRFYLESHVRSGGWEWIQRRNPASKYPTDFGLVSIEDSVRGELIEEIE 124

Query: 124  ELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAAISNA 183
            ELELVKDVNVDASH RGL+TEDGGRVGAFVDGKKRPGKIF+SMSF E GGE Y+ AI+NA
Sbjct: 125  ELELVKDVNVDASHVRGLLTEDGGRVGAFVDGKKRPGKIFTSMSFKEGGGEHYT-AITNA 184

Query: 184  SNRWGRHLSMQRSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHPHFKNIKERTNWT 243
            SNRWGRHLSM+RSQVTSLFGADSLW+KGYTG KVKMAIFDTGIRA+HPHF+NIKERTNWT
Sbjct: 185  SNRWGRHLSMERSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIKERTNWT 244

Query: 244  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 303
            NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI
Sbjct: 245  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 304

Query: 304  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 363
            ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG
Sbjct: 305  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 364

Query: 364  VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 423
            VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS
Sbjct: 365  VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 424

Query: 424  PVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQGAGRVNLLESYEI 483
            PVVAGVVCLLVSVIPESQRK+ILNPASMKQALVEGAAKLAGPN+YEQGAGRV+LLESYE+
Sbjct: 425  PVVAGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEV 484

Query: 484  LKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGMGVIGYVESQPTW 543
            LKSYQPRASIFP VLDYTDCPY+WPFCRQPLYAGAMPIIFNATILNGMGVIGYVE QPTW
Sbjct: 485  LKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTW 544

Query: 544  HPLNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS-PSSGE 603
            HP ++EGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS PS GE
Sbjct: 545  HPSDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGE 604

Query: 604  KNRQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHL 663
            KNR+ STCVLQLKLKVVPTPPRSKR+LWDQFH+IKYPPGYIPRDSLDVRNDILDWHGDHL
Sbjct: 605  KNRRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHL 664

Query: 664  HTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 723
            HTNFHIMFNMLRDAGYYVE+LGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVM T
Sbjct: 665  HTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTT 724

Query: 724  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 783
            GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG
Sbjct: 725  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 784

Query: 784  DFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILTSSMNKAEFPILGLLE 843
            DFSIDGEQSRYASGTDIVRFPQGGY+HSFPFVDSSESG AQSILTSSM+KA+FPILGLLE
Sbjct: 785  DFSIDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLE 844

Query: 844  AVEGRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDPVLFTKSSKRNSPLYLEDN 903
            A EGRIAVYGDSNCLDSSHMVTNCYWLL+KILDFTS NIRDP+LFTK SKRNSPLYLED+
Sbjct: 845  AGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDS 904

Query: 904  KLPSRRSDVNFSTYSAVVGKELICRSDSRFEVWGTKGYSIHVRGRNRRLPGFPVFDLGRG 963
            KLPSRRSDVNFS YSAV  KELICRSDSRFEVWGTKGYSI VRGRNRRLPGFPV DLGRG
Sbjct: 905  KLPSRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRG 964

Query: 964  LNYS---TSTRPPKLTLKDRTDTYANRYLSLFYRDEPGAPLIVPNHWLVPAIVALTGLLL 1023
            LN +   +S  PPK + KDR+DTY NRYLSLFYRDEP  PLIVPNHWLVPA+VALTGL L
Sbjct: 965  LNSTSEGSSMGPPKSSSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFL 1024

Query: 1024 ILSFWRIRQKRRRRRRGSGSARFPNL 1043
            +LSFWRIRQKRRRRRRGSGSARF N+
Sbjct: 1025 LLSFWRIRQKRRRRRRGSGSARFSNI 1045

BLAST of Sed0019002 vs. ExPASy TrEMBL
Match: A0A1S3CJL6 (subtilisin-like protease SBT6.1 OS=Cucumis melo OX=3656 GN=LOC103501563 PE=3 SV=1)

HSP 1 Score: 1925.6 bits (4987), Expect = 0.0e+00
Identity = 945/1045 (90.43%), Postives = 987/1045 (94.45%), Query Frame = 0

Query: 4    SSISFIII---IFISISFFQFKPSSDHTVFQTLARNHSPADPFPANNLTHGNRGSRNKNY 63
            SS+SFI I   IFISIS FQFKP   +T+FQTL  N+S  DP P NNLTH N   R +NY
Sbjct: 5    SSVSFIAIFLPIFISISLFQFKP---NTLFQTLTPNYSTFDPSPFNNLTHRNSVLRKQNY 64

Query: 64   IVRFVQYREAKEHRFYLESRVRSGGWGWIERRNAASKYPTDFGLVSIEESVRRELMEEIE 123
            IVRF+QYR+AK+HRFYLESRVRSGGW WI+RRN ASKYPTDFGLVSIE+SVR EL+EEIE
Sbjct: 65   IVRFLQYRQAKDHRFYLESRVRSGGWEWIQRRNPASKYPTDFGLVSIEDSVRGELIEEIE 124

Query: 124  ELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAAISNA 183
            ELELVKDVNVDASH RGL+TED GRVGAFVDGKKRPGKIF+SMSF E GGERY+ AI+NA
Sbjct: 125  ELELVKDVNVDASHVRGLLTEDAGRVGAFVDGKKRPGKIFTSMSFKEGGGERYT-AITNA 184

Query: 184  SNRWGRHLSMQRSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHPHFKNIKERTNWT 243
            SNRWGRHLSM+RSQVTSLFGADSLW+KGYTG KVKMAIFDTGIRA+HPHF+NIKERTNWT
Sbjct: 185  SNRWGRHLSMERSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIKERTNWT 244

Query: 244  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 303
            NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI
Sbjct: 245  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 304

Query: 304  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 363
            ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG
Sbjct: 305  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 364

Query: 364  VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 423
            VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS
Sbjct: 365  VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 424

Query: 424  PVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQGAGRVNLLESYEI 483
            PVVAGVVCLLVSVIPESQRK+ILNPASMKQALVEGAAKLAGPN+YEQGAGRV+LLESYE+
Sbjct: 425  PVVAGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEV 484

Query: 484  LKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGMGVIGYVESQPTW 543
            LKSYQPRASIFP VLDYTDCPY+WPFCRQPLYAGAMPIIFNATILNGMGVIGYVE QPTW
Sbjct: 485  LKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTW 544

Query: 544  HPLNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS-PSSGE 603
            HP ++E NLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS PS GE
Sbjct: 545  HPSDEEANLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGE 604

Query: 604  KNRQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHL 663
            KNR+ STCVLQLKLKVVPTPPRSKR+LWDQFH+IKYPPGYIPRDSLDVRNDILDWHGDHL
Sbjct: 605  KNRRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHL 664

Query: 664  HTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 723
            HTNFHIMFNMLRDAGYYVE+LGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVM T
Sbjct: 665  HTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTT 724

Query: 724  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 783
            GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG
Sbjct: 725  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 784

Query: 784  DFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILTSSMNKAEFPILGLLE 843
            DFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESG AQSILTSSM+KA+F ILGLLE
Sbjct: 785  DFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGAAQSILTSSMSKADFSILGLLE 844

Query: 844  AVEGRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDPVLFTKSSKRNSPLYLEDN 903
            A EGRIAVYGDSNCLDSSHMVTNCYWLL+KILDFTSGNIRDPVLFTK SKR +PLYLED+
Sbjct: 845  AAEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKRKTPLYLEDS 904

Query: 904  KLPSRRSDVNFSTYSAVVGKELICRSDSRFEVWGTKGYSIHVRGRNRRLPGFPVFDLGRG 963
            KLPSRRSDVNFS YSAV GKELICRSDSRFEVWGTKGYS  VRGRNRRLPGFPV DLGRG
Sbjct: 905  KLPSRRSDVNFSLYSAVAGKELICRSDSRFEVWGTKGYSTQVRGRNRRLPGFPVIDLGRG 964

Query: 964  LNYS--TSTRPPKLTLKDRTDTYANRYLSLFYRDEPGAPLIVPNHWLVPAIVALTGLLLI 1023
            LN +  +S  PPK T KDR+DTY NRYLSLFYRDEP  PL VPNHWLVPA+VALTGLLL+
Sbjct: 965  LNSTSESSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLSVPNHWLVPAVVALTGLLLL 1024

Query: 1024 LSFWRIRQKRRRRRRGSGSARFPNL 1043
            LSFWRIRQKRRRRRRGSGSARF N+
Sbjct: 1025 LSFWRIRQKRRRRRRGSGSARFSNI 1045

BLAST of Sed0019002 vs. ExPASy TrEMBL
Match: A0A6J1CUP3 (subtilisin-like protease SBT6.1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014445 PE=3 SV=1)

HSP 1 Score: 1911.0 bits (4949), Expect = 0.0e+00
Identity = 935/1047 (89.30%), Postives = 992/1047 (94.75%), Query Frame = 0

Query: 4    SSISFIII---IFISISFFQFKPSSDHTVFQTLARNHSPADPFPANNLTHGNRGSRNKNY 63
            S +SF++I   IFISIS FQFKPSSD  V+QTL RN+S  DPF + NL+ GN+ SR KNY
Sbjct: 9    SYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGNQVSR-KNY 68

Query: 64   IVRFVQYREAKEHRFYLESRVRSGGWGWIERRNAASKYPTDFGLVSIEESVRRELMEEIE 123
            IVRFVQYREAKEHR YLESR+RSG W WIER+N ASKYPT+FGLVSIE+S R EL+++IE
Sbjct: 69   IVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARGELIKDIE 128

Query: 124  ELELVKDVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAAISNA 183
            ELELVKDVNVDAS+ R ++TE+GGRVGAFVDGKKRPGKIF+SMSF E GGERY+ AISNA
Sbjct: 129  ELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERYT-AISNA 188

Query: 184  SNRWGRHLSMQRSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHPHFKNIKERTNWT 243
            SNRWGRHLSMQRSQVTSLFGA+SLW+KGYTG KV+MAIFDTGIRA+HPHF+NIKERTNWT
Sbjct: 189  SNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIKERTNWT 248

Query: 244  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 303
            NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI
Sbjct: 249  NEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAI 308

Query: 304  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 363
            ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG
Sbjct: 309  ATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIG 368

Query: 364  VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 423
            VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS
Sbjct: 369  VGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVAS 428

Query: 424  PVVAGVVCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQGAGRVNLLESYEI 483
            PVVAGVVCLLVSVIPESQR+ ILNPASMKQALVEGAAKLAGPN+YEQGAGRV+LLESYEI
Sbjct: 429  PVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDLLESYEI 488

Query: 484  LKSYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGMGVIGYVESQPTW 543
            LKSYQPRASIFP+VLDYTDCPY WPFCRQPLYAGAMPIIFNATILNGMGVIGYVE QPTW
Sbjct: 489  LKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTW 548

Query: 544  HPLNDEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS-PSSGE 603
            HP ++EGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS PSSGE
Sbjct: 549  HPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSSGE 608

Query: 604  KNRQTSTCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHL 663
            KNR+TSTCVL LKLKVVPTPPRSKR+LWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHL
Sbjct: 609  KNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHL 668

Query: 664  HTNFHIMFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMIT 723
            HTNFHIMFNMLRDAGYYVE+LGSPLTCFD+ QYGTLLLVDLEDEYFKEEIEKLRDDVM+T
Sbjct: 669  HTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLRDDVMVT 728

Query: 724  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 783
            GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG
Sbjct: 729  GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 788

Query: 784  DFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILTSSMNKAEFPILGLLE 843
            DFSIDGEQSRYASGTDIVRFP+GGYVHSFPF+DSSESG AQ+ILTS M KA+FPILGLLE
Sbjct: 789  DFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFPILGLLE 848

Query: 844  AV-EGRIAVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDPVLFTKSSKRNSPLYLED 903
             V EGRIAVYGDSNCLDSSHMVTNCYWLL+KILDFTSGNIRDPVLFTKSSK +SPLYLED
Sbjct: 849  EVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDSPLYLED 908

Query: 904  NKLPSRRSDVNFSTYSAVVGKELICRSDSRFEVWGTKGYSIHVRGRNRRLPGFPVFDLGR 963
            NKLPSRRSDVNFS YS VVGKELICRSDSRF+ WGTKGY+I VRGRNRRLPGFP+ DLGR
Sbjct: 909  NKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFPIIDLGR 968

Query: 964  GLNYSTST---RPPKLTLKDRTDTYANRYLSLFYRDEPGAPLIVPNHWLVPAIVALTGLL 1023
            GLN ++ T   RPPKLT ++R+D+Y NRYL+LFY DEP  PLIVPNHWLVPA+VA+TGLL
Sbjct: 969  GLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVVAVTGLL 1028

Query: 1024 LILSFWRIRQKRRRRRRGSGSARFPNL 1043
            L+LSFWRIRQ+RRRRRRGSGSARFPNL
Sbjct: 1029 LLLSFWRIRQRRRRRRRGSGSARFPNL 1053

BLAST of Sed0019002 vs. TAIR 10
Match: AT5G19660.1 (SITE-1 protease )

HSP 1 Score: 1602.0 bits (4147), Expect = 0.0e+00
Identity = 778/1038 (74.95%), Postives = 885/1038 (85.26%), Query Frame = 0

Query: 7    SFIIIIFISISFFQFKPSSDHTVFQTLARNHSPADPFPANNLTHGNRGSRNKNYIVRFVQ 66
            S II++F+S+S F  +PS+ H   Q L          P N     +      NYI+RF Q
Sbjct: 14   SCIIVVFLSVSLFWLRPSTYHPQQQNLN---------PENVTRLESENETKTNYIIRFKQ 73

Query: 67   YREAKEHRFYLESRVRSGGWGWIERRNAASKYPTDFGLVSIEESVRRELMEEIEELELVK 126
            Y+ AK+HR YLES+VRSGGWGWIER N A+KYPTDFG++ IEES +  ++ EIE LE+VK
Sbjct: 74   YKPAKDHRIYLESKVRSGGWGWIERINPATKYPTDFGVLWIEESGKEAVVGEIERLEMVK 133

Query: 127  DVNVDASHARGLVTEDGGRVGAFVDGKKRPGKIFSSMSFNEEGGERYSAAISNASNRWGR 186
            DVNV+  + R L+       G+F DGKKRPGKIF+SMSF E       A  SN +  W R
Sbjct: 134  DVNVEFKYQRVLLG------GSFPDGKKRPGKIFTSMSFEEGTESSPMADTSNTTLNWSR 193

Query: 187  HLSMQRSQVTSLFGADSLWSKGYTGCKVKMAIFDTGIRASHPHFKNIKERTNWTNEDTLN 246
            HL  Q++QVTS+FGAD LW KGYTG KVKMAIFDTGIRA HPHF+ IKERTNWTNEDTLN
Sbjct: 194  HLLAQKTQVTSMFGADHLWKKGYTGAKVKMAIFDTGIRADHPHFRKIKERTNWTNEDTLN 253

Query: 247  DNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAIATNMDV 306
            DNLGHGTFVAGVIAG + ECLGFA DTEIYAFRVFTDAQVSYTSWFLDAFNYAIAT+MDV
Sbjct: 254  DNLGHGTFVAGVIAGRNPECLGFASDTEIYAFRVFTDAQVSYTSWFLDAFNYAIATDMDV 313

Query: 307  LNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGGIDY 366
            LNLSIGGPDYLDLPFVEK+WEITA+NIIMVSAIGNDGPLYGTLNNPADQSDVIGVGGID 
Sbjct: 314  LNLSIGGPDYLDLPFVEKVWEITASNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGGIDN 373

Query: 367  NDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVVAGV 426
            +DHIASFSSRGM+TWE+PHGYGRVKPDVVAYGR+IMGSKISTGCKSLSGTSVASPVVAG+
Sbjct: 374  DDHIASFSSRGMSTWELPHGYGRVKPDVVAYGRDIMGSKISTGCKSLSGTSVASPVVAGI 433

Query: 427  VCLLVSVIPESQRKIILNPASMKQALVEGAAKLAGPNLYEQGAGRVNLLESYEILKSYQP 486
            VCLLVSVIPE++RK +LNPASMKQALVEGAAKL+GPN+YEQGAGRV+LLESYEILKSY P
Sbjct: 434  VCLLVSVIPEARRKDLLNPASMKQALVEGAAKLSGPNMYEQGAGRVDLLESYEILKSYHP 493

Query: 487  RASIFPSVLDYTDCPYSWPFCRQPLYAGAMPIIFNATILNGMGVIGYVESQPTWHPLNDE 546
            RASIFPS+LDY DCPYSWPFCRQPLYAGAMPIIFN TILNGMGVIGY+ES PTWHP N+E
Sbjct: 494  RASIFPSILDYNDCPYSWPFCRQPLYAGAMPIIFNTTILNGMGVIGYIESPPTWHPANEE 553

Query: 547  GNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS-PSSGEKNRQTS 606
            GNLLSIHF Y  VIWPWTGY+ALHMQIKEEGAQF+GEIEGNVT+ VYS P+SGE   + S
Sbjct: 554  GNLLSIHFKYPDVIWPWTGYLALHMQIKEEGAQFTGEIEGNVTVKVYSPPASGESGPRRS 613

Query: 607  TCVLQLKLKVVPTPPRSKRVLWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHLHTNFHI 666
            TC LQLKLKV+PTPPR+KR+LWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHLHTNFHI
Sbjct: 614  TCSLQLKLKVIPTPPRAKRILWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHLHTNFHI 673

Query: 667  MFNMLRDAGYYVESLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMITGLGLAV 726
            M+NMLRDAGYY+E+LGSPLTCFDA+QYGTLL+VDLED+YF EEIEKLRDDV+ TGLGL V
Sbjct: 674  MYNMLRDAGYYIETLGSPLTCFDAQQYGTLLMVDLEDDYFPEEIEKLRDDVINTGLGLVV 733

Query: 727  FSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFSIDG 786
            F+EWYNV+TMVKMRFFDDNTRSWWTPVTGGANIPALN+LLA FGIAFGDKILNGDFSIDG
Sbjct: 734  FAEWYNVDTMVKMRFFDDNTRSWWTPVTGGANIPALNNLLASFGIAFGDKILNGDFSIDG 793

Query: 787  EQSRYASGTDIVRFPQGGYVHSFPFVDSSESGVAQSILTSSMNKAEFPILGLLEAVEGRI 846
            EQSRYASGT+IVRFP GG++H+FP +DSSESG  Q++L +  +K +  +LGLLE  EGR+
Sbjct: 794  EQSRYASGTNIVRFPAGGFLHTFPLLDSSESGATQNLLLTEASKEDPAVLGLLEIGEGRV 853

Query: 847  AVYGDSNCLDSSHMVTNCYWLLKKILDFTSGNIRDPVLFTKSSKRNSPLYLEDNKLPSRR 906
             VYGDSNCLDSSHMVTNCYWLLKK+LDF+S NI+DPVLF+K +KR SP+ +++ +LPSRR
Sbjct: 854  GVYGDSNCLDSSHMVTNCYWLLKKMLDFSSSNIKDPVLFSKFAKRYSPVIIDEKQLPSRR 913

Query: 907  SDVNFSTYSAVVGKELICRSDSRFEVWGTKGYSIHVRGRNRRLPGFPVFDLGRGLNYSTS 966
            +DVNFSTYS+V+GKELIC SDSRFEVWGTKGY++HVRGRNRRLPG+   DLGRGLN++  
Sbjct: 914  TDVNFSTYSSVIGKELICESDSRFEVWGTKGYNLHVRGRNRRLPGYHGIDLGRGLNFTVE 973

Query: 967  TRPPK--LTLKDRTDTYANRYLS---LFYRDEPGAPLIVPNHWLVPAIVALTGLLLILSF 1026
            ++ P    + K+  +  ++R  S   LF RDE   P +VP  W+V A V  +G+L++LS 
Sbjct: 974  SKRPTRWRSAKEGGELSSSRSKSLGGLFNRDEIDMPFLVPTRWIVLAGVVASGVLVLLSI 1033

Query: 1027 WRIRQKRRRRRRGSGSAR 1039
            WRIRQKR RRRR SGS R
Sbjct: 1034 WRIRQKRGRRRRASGSNR 1036

BLAST of Sed0019002 vs. TAIR 10
Match: AT1G20160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 3.1e-07
Identity = 43/138 (31.16%), Postives = 64/138 (46.38%), Query Frame = 0

Query: 244 TLNDNLGHGTFVAGVIAGGDEE-----------CLGFAPDTEIYAFRVFTDAQVSYTSWF 303
           T  D +GHG+ V+  IAG   E             G + +  I  ++V      + +S  
Sbjct: 203 TTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAKGGSQNARIAMYKVCNPGGCTGSS-I 262

Query: 304 LDAFNYAIATNMDVLNLSIGGPDY--LDL---PFVEKIWEITANNIIMVSAIGNDGPLYG 363
           L AF+ AIA  +DVL+LS+G P Y  +DL   P     +      I+++ + GNDGP  G
Sbjct: 263 LAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGAFHAVEQGILVICSAGNDGPDGG 322

Query: 364 TLNNPADQSDVIGVGGID 366
           T+ N A     +    ID
Sbjct: 323 TVTNTAPWIMTVAANTID 339

BLAST of Sed0019002 vs. TAIR 10
Match: AT1G20160.2 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 3.1e-07
Identity = 43/138 (31.16%), Postives = 64/138 (46.38%), Query Frame = 0

Query: 244 TLNDNLGHGTFVAGVIAGGDEE-----------CLGFAPDTEIYAFRVFTDAQVSYTSWF 303
           T  D +GHG+ V+  IAG   E             G + +  I  ++V      + +S  
Sbjct: 164 TTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAKGGSQNARIAMYKVCNPGGCTGSS-I 223

Query: 304 LDAFNYAIATNMDVLNLSIGGPDY--LDL---PFVEKIWEITANNIIMVSAIGNDGPLYG 363
           L AF+ AIA  +DVL+LS+G P Y  +DL   P     +      I+++ + GNDGP  G
Sbjct: 224 LAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGAFHAVEQGILVICSAGNDGPDGG 283

Query: 364 TLNNPADQSDVIGVGGID 366
           T+ N A     +    ID
Sbjct: 284 TVTNTAPWIMTVAANTID 300

BLAST of Sed0019002 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 3.1e-07
Identity = 44/133 (33.08%), Postives = 61/133 (45.86%), Query Frame = 0

Query: 247 DNLGHGTFVAGVIAG-----------GDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDA 306
           D+ GHGT  A + AG                 G AP   + A++V  ++   Y S  L A
Sbjct: 206 DSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSG-CYDSDILAA 265

Query: 307 FNYAIATNMDVLNLSIGG---PDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNP 366
           F+ A+A  +DV++LS+GG   P YLD   +     I     +  SA GN GP   T+ N 
Sbjct: 266 FDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASA-GNGGPGALTVTNV 325

BLAST of Sed0019002 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 49.7 bits (117), Expect = 1.7e-05
Identity = 40/135 (29.63%), Postives = 57/135 (42.22%), Query Frame = 0

Query: 250 GHGTFVAGVIAG-----------GDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNY 309
           GHGT  +   AG                 G AP   I A++V         S  L AF+ 
Sbjct: 204 GHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDA 263

Query: 310 AIATNMDVLNLSIGG------PDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNP 368
           A+   +DV+++SIGG      P YLD P     +   +  I + S+ GN+GP   ++ N 
Sbjct: 264 AVRDGVDVISISIGGGDGITSPYYLD-PIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNL 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879333.10.0e+0091.40subtilisin-like protease SBT6.1 [Benincasa hispida][more]
KAG6589917.10.0e+0091.02Subtilisin-like protease 6.1, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_023516371.10.0e+0090.93subtilisin-like protease SBT6.1 [Cucurbita pepo subsp. pepo][more]
XP_022960716.10.0e+0090.83subtilisin-like protease SBT6.1 [Cucurbita moschata][more]
XP_022987592.10.0e+0090.74subtilisin-like protease SBT6.1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q0WUG60.0e+0074.95Subtilisin-like protease SBT6.1 OS=Arabidopsis thaliana OX=3702 GN=SBT6.1 PE=1 S... [more]
Q147031.1e-25451.14Membrane-bound transcription factor site-1 protease OS=Homo sapiens OX=9606 GN=M... [more]
Q9WTZ22.3e-25451.02Membrane-bound transcription factor site-1 protease OS=Mus musculus OX=10090 GN=... [more]
Q9WTZ33.1e-25451.14Membrane-bound transcription factor site-1 protease OS=Rattus norvegicus OX=1011... [more]
Q9Z2A86.8e-25451.02Membrane-bound transcription factor site-1 protease OS=Cricetulus griseus OX=100... [more]
Match NameE-valueIdentityDescription
A0A6J1H8D80.0e+0090.83subtilisin-like protease SBT6.1 OS=Cucurbita moschata OX=3662 GN=LOC111461434 PE... [more]
A0A6J1JAS50.0e+0090.74subtilisin-like protease SBT6.1 OS=Cucurbita maxima OX=3661 GN=LOC111485106 PE=3... [more]
A0A0A0LTP50.0e+0090.34Peptidase_S8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G25016... [more]
A0A1S3CJL60.0e+0090.43subtilisin-like protease SBT6.1 OS=Cucumis melo OX=3656 GN=LOC103501563 PE=3 SV=... [more]
A0A6J1CUP30.0e+0089.30subtilisin-like protease SBT6.1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT5G19660.10.0e+0074.95SITE-1 protease [more]
AT1G20160.13.1e-0731.16Subtilisin-like serine endopeptidase family protein [more]
AT1G20160.23.1e-0731.16Subtilisin-like serine endopeptidase family protein [more]
AT3G14240.13.1e-0733.08Subtilase family protein [more]
AT4G34980.11.7e-0529.63subtilisin-like serine protease 2 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 105..125
NoneNo IPR availablePANTHERPTHR43806:SF7MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-1 PROTEASEcoord: 56..869
NoneNo IPR availablePANTHERPTHR43806PEPTIDASE S8coord: 56..869
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 187..481
score: 33.935356
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 247..260
score: 52.92
coord: 211..230
score: 36.36
coord: 414..430
score: 51.94
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 188..479
e-value: 7.7E-69
score: 234.2
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 196..483
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 213..462
e-value: 1.2E-43
score: 149.4
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 415..425
IPR022398Peptidase S8, subtilisin, His-active sitePROSITEPS00137SUBTILASE_HIScoord: 251..261
IPR034185Site-1 peptidase catalytic domainCDDcd07479Peptidases_S8_SKI-1_likecoord: 205..461
e-value: 0.0
score: 530.103

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0019002.1Sed0019002.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity