Sed0018597 (gene) Chayote v1

BLAST of Sed0018597 vs. NCBI nr
Match: XP_022962970.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like [Cucurbita moschata] >KAG6594890.1 RNA cytosine C(5)-methyltransferase NSUN2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026856.1 tRNA (cytosine(34)-C(5))-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1419.1 bits (3672), Expect = 0.0e+00
Identity = 719/849 (84.69%), Postives = 767/849 (90.34%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTHWEPFESQSQSPAFNE 116
           MG KGRG RARTQR+HFRE R+NVWK  KPDPNSANG  DGNG HWEPFESQS   AFNE
Sbjct: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSH--AFNE 60

Query: 117 YYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVT--D 176
           YYKEQG+VTAEEWD FIE LRKPLPAAFRINSSGQFCGEIRAQLQNDF +SLQAEVT  D
Sbjct: 61  YYKEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGD 120

Query: 177 EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 236
           EAEAIRPLPWY DNLAWHSNFSRMQLRKNQALE FHEFLKLENEIGNITRQEAVSMVPPL
Sbjct: 121 EAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPL 180

Query: 237 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIHQTK 296
           FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSK GDLP+GLVVANDLDVQRCNLLIHQTK
Sbjct: 181 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTK 240

Query: 297 RMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGDGTL 356
           RMCTANLIVTNHEAQHFPGCRANTN F+AS AGNELK  +TQLTFDRVLCDVPCSGDGTL
Sbjct: 241 RMCTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTL 300

Query: 357 RKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 416
           RKAPDIW++WNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR
Sbjct: 301 RKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360

Query: 417 KCGGSLELVDVSNELPQLVRRPGLKKWKVLDRCVWLVTYKQVIERRQSMAIPSMFPSGRG 476
           K GGSLELVDVSNELPQLVRRPGLKKWKV D+ VW  +Y+QV E  QS+AIP MFPSGRG
Sbjct: 361 KSGGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRG 420

Query: 477 CKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMRIVPHDQN 536
            K+   D HNSELR NH DG  GSSVD+SEPT + VH FDEEVCEFPIERCMRIVPHDQN
Sbjct: 421 RKS---DTHNSELRGNHPDG--GSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQN 480

Query: 537 SGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISATDGM-DRS 596
           +GAFF+AVL+KVAPLP ATEK S+Q +KSAN++  GKL+Q ITDA+SVD++ATDGM + S
Sbjct: 481 TGAFFVAVLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESS 540

Query: 597 PEVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEVDLVVNG 656
            EV+ID NNQ  SN A+N PPC+LPVQD +D DGLDS   DGMDK++SE D+EV  V N 
Sbjct: 541 SEVEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLDS--TDGMDKQFSEADMEV--VENE 600

Query: 657 MSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDPVVIFNDETVIDS 716
            +ENKLEITAI +NSE+S+QP DGE DPKK N K KLQIQGKWRGVDPVV+F DETVID 
Sbjct: 601 TTENKLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDC 660

Query: 717 IKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQLKITSIGVKM 776
           IKTFYGI+ESFPLIGHLVTRNSDTNHVKRIYYIS SVKDVLELNFSAGQQLKITSIG+KM
Sbjct: 661 IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKM 720

Query: 777 FERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSIKCADFVDAA 836
           FERQSS+EGSSAPC FRISSEGLPV+LPYITKQILTMSPVDFKHLL++KSIK ADFVDAA
Sbjct: 721 FERQSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAA 780

Query: 837 FGEKASNLMLGCCVIVLGTGNTASDVIGVDESTIAIGCWKGRASLSVMVTAIDCQELLGR 896
           FGEKASNLMLGCCVIVLGTGNT+SDVI VDESTIAIGCWKGRASLSVMVTA DCQELLGR
Sbjct: 781 FGEKASNLMLGCCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGR 838

Query: 897 LSNQLGNDL 903
           LSN+LGNDL
Sbjct: 841 LSNRLGNDL 838

BLAST of Sed0018597 vs. NCBI nr
Match: XP_023003087.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like [Cucurbita maxima])

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 720/849 (84.81%), Postives = 768/849 (90.46%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTHWEPFESQSQSPAFNE 116
           MG KGRG RARTQR+HFRE R+NVWK  KPDPNSAN   DGNG HWEPFE  SQSPAFNE
Sbjct: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANDNKDGNGNHWEPFE--SQSPAFNE 60

Query: 117 YYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVT--D 176
           YYKEQG+VTAEEWD FIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDF++SLQAEVT  D
Sbjct: 61  YYKEQGIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFSRSLQAEVTDGD 120

Query: 177 EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 236
           EAEAIRPLPWY DNLAWHSNFSRMQLRKNQALE FHEFLKLENEIGNITRQEAVSMVPPL
Sbjct: 121 EAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPL 180

Query: 237 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIHQTK 296
           FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSK GDLP+GLVVANDLDVQRCNLLIHQTK
Sbjct: 181 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTK 240

Query: 297 RMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGDGTL 356
           RMCTANLIVTNHEAQHFPGCRANTN F+AS AGNELK  +TQLTFDRVLCDVPCSGDGTL
Sbjct: 241 RMCTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTL 300

Query: 357 RKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 416
           RKAPDIW++WNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR
Sbjct: 301 RKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360

Query: 417 KCGGSLELVDVSNELPQLVRRPGLKKWKVLDRCVWLVTYKQVIERRQSMAIPSMFPSGRG 476
           K GGSLELVDVSNELPQLVRRPGLKKWKV D+ VW  +Y+QV E  QS+AIP MFPSGRG
Sbjct: 361 KSGGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRG 420

Query: 477 CKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMRIVPHDQN 536
            K+   D HNS+LR NH DG  GSSVD+SEPT + VH FDEEVCEFPIERCMRIVPHDQN
Sbjct: 421 HKS---DTHNSQLRGNHPDG--GSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQN 480

Query: 537 SGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISATDGM-DRS 596
           +GAFF+AVL+KVAPL  ATEK S+Q +KSAN++ RGKL+Q ITDA+SVD++ATDGM + S
Sbjct: 481 TGAFFVAVLRKVAPLLAATEKTSDQNTKSANDQSRGKLQQTITDADSVDLNATDGMGESS 540

Query: 597 PEVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEVDLVVNG 656
            EV+ID NNQ  SN A+N PPC+LPVQD +D DGLDS   DGMDK +SE DLEV  V N 
Sbjct: 541 SEVEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLDS--TDGMDKPFSEADLEV--VDNE 600

Query: 657 MSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDPVVIFNDETVIDS 716
            +ENKLEITAI +NSE+S+QP DGE DPKK N K KLQIQGKWRGVDPVV+F DETVID 
Sbjct: 601 TTENKLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDC 660

Query: 717 IKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQLKITSIGVKM 776
           IKTFYGI+ESFPLIGHLVTRNSDTNHVKRIYYIS SVKDVLELNFSAGQQLKITSIG+KM
Sbjct: 661 IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKM 720

Query: 777 FERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSIKCADFVDAA 836
           FERQSS+EGSSAPC FRISSEGLPV+LPYITKQILTMSPVDFKHLL++KSIK ADFVDAA
Sbjct: 721 FERQSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAA 780

Query: 837 FGEKASNLMLGCCVIVLGTGNTASDVIGVDESTIAIGCWKGRASLSVMVTAIDCQELLGR 896
           FGEKASNLMLGCCVIVLGTGNT+SDVI VDESTIAIGCWKGRASLSVMVTA DCQELLGR
Sbjct: 781 FGEKASNLMLGCCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGR 838

Query: 897 LSNQLGNDL 903
           LSN+LGNDL
Sbjct: 841 LSNRLGNDL 838

BLAST of Sed0018597 vs. NCBI nr
Match: XP_023517733.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1412.9 bits (3656), Expect = 0.0e+00
Identity = 716/849 (84.33%), Postives = 766/849 (90.22%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTHWEPFESQSQSPAFNE 116
           MG KGRG RARTQR+HFRE R+NVWK  KPDPNSAN   DGNG HWEPFESQS   AFNE
Sbjct: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANDNKDGNGNHWEPFESQSH--AFNE 60

Query: 117 YYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVT--D 176
           YYKEQG+VTAEEW+ FIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDF +SLQAEVT  D
Sbjct: 61  YYKEQGIVTAEEWNTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGD 120

Query: 177 EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 236
           EAEAIRPLPWY DNLAWHSNFSRMQLRKNQALE FHEFLKLENEIGNITRQEAVSMVPPL
Sbjct: 121 EAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPL 180

Query: 237 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIHQTK 296
           FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSK GDLP+GLVVANDLDVQRCNLLIHQTK
Sbjct: 181 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTK 240

Query: 297 RMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGDGTL 356
           RMCTANLIVTNHEAQHFPGCRANTN F+AS AGNELK  +TQLTFDRVLCDVPCSGDGTL
Sbjct: 241 RMCTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTL 300

Query: 357 RKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 416
           RKAPDIW++WNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR
Sbjct: 301 RKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360

Query: 417 KCGGSLELVDVSNELPQLVRRPGLKKWKVLDRCVWLVTYKQVIERRQSMAIPSMFPSGRG 476
           K GGSLELVDVSNELPQLVRRPGLKKWKV D+ VW  +Y+QV E  QS+AIP MFPSGRG
Sbjct: 361 KSGGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRG 420

Query: 477 CKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMRIVPHDQN 536
            K+   D HNSELR NH DG  GSSVD+SEPT + VH FDEEVCEFPIERCMRIVPHDQN
Sbjct: 421 RKS---DTHNSELRGNHPDG--GSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQN 480

Query: 537 SGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISATDGM-DRS 596
           +GAFF+AVL+KVAPLP ATEK S+Q +KSAN++ RGKL+Q  TDA+SVD++ATDGM + S
Sbjct: 481 TGAFFVAVLRKVAPLPAATEKTSDQNTKSANDQSRGKLQQTTTDADSVDLNATDGMGESS 540

Query: 597 PEVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEVDLVVNG 656
            EV+ID NNQ  SN A+N PPC+LPVQD +D DGLDS   DGMDK++SE D+EV  V N 
Sbjct: 541 SEVEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLDS--TDGMDKQFSEADMEV--VENE 600

Query: 657 MSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDPVVIFNDETVIDS 716
            +ENKLEITAI +NSE+S+QP DGE DPKK N K KLQIQGKWRGVDPVV+F DETVID 
Sbjct: 601 TTENKLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDC 660

Query: 717 IKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQLKITSIGVKM 776
           IKTFYGI+ESFPLIGHLVTRNSDTNHVKRIYYIS SVKDVLELNFSAGQQLKITSIG+KM
Sbjct: 661 IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKM 720

Query: 777 FERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSIKCADFVDAA 836
           FERQSS+EGSSAPC FRISSEGLPV+LPYITKQILTMSPVDFKHLL++KSIK ADFVDAA
Sbjct: 721 FERQSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAA 780

Query: 837 FGEKASNLMLGCCVIVLGTGNTASDVIGVDESTIAIGCWKGRASLSVMVTAIDCQELLGR 896
           FGEKASNLMLGCCVIVLG GNT+S+VI VDESTIAIGCWKGRASLSVMVTA DCQELLGR
Sbjct: 781 FGEKASNLMLGCCVIVLGAGNTSSEVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGR 838

Query: 897 LSNQLGNDL 903
           LSN+LGNDL
Sbjct: 841 LSNRLGNDL 838

BLAST of Sed0018597 vs. NCBI nr
Match: XP_022950404.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1408.3 bits (3644), Expect = 0.0e+00
Identity = 712/850 (83.76%), Postives = 761/850 (89.53%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTHWEPFESQSQSPAFNE 116
           MG KGRG RARTQR HFRE R+NVWK SKPD NSAN   DGN  HW PF  QSQS AFNE
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPF--QSQSHAFNE 60

Query: 117 YYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVT--D 176
           YYKEQG+VTA EWD FIEVLRK LPAAFRINSSGQFCGEIRAQLQNDF +SLQ+EVT  D
Sbjct: 61  YYKEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGD 120

Query: 177 EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 236
           EA AIRPL WY DNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL
Sbjct: 121 EAGAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 180

Query: 237 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIHQTK 296
           FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSK GDLP GLVVAND DVQRCNLLIHQTK
Sbjct: 181 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTK 240

Query: 297 RMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGDGTL 356
           RMCT+NLIVTNHEAQHFPGCRA+TN+FNAS AG+EL  HNTQLTFDRVLCDVPCSGDGTL
Sbjct: 241 RMCTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDGTL 300

Query: 357 RKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 416
           RKAPDIW+RWNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR
Sbjct: 301 RKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360

Query: 417 KCGGSLELVDVSNELPQLVRRPGLKKWKVLDRCVWLVTYKQVIERRQSMAIPSMFPSGRG 476
           K  GSLELVDVSNELPQLVRRPGLKKWKV DR VWL +YKQV + RQS+AIPSMFPSGRG
Sbjct: 361 KSEGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRG 420

Query: 477 CKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMRIVPHDQN 536
            KNQA  +HNSELR NHLDG+ GSSVD+SEPT + VHEFD EVCEFPIERCMRIVPHDQN
Sbjct: 421 RKNQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFD-EVCEFPIERCMRIVPHDQN 480

Query: 537 SGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISATDGMDRSP 596
           SGAFFIAVL+KVAPLP ATEKPS Q  K  N++ R KL Q IT+  +VD+SA+ GMD SP
Sbjct: 481 SGAFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESP 540

Query: 597 -EVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEVDLVVNG 656
            EV +D+NNQG  N ADNKP CE PVQDIE  +GLDSNAADG+DK++SE DLEV+LV N 
Sbjct: 541 SEVGVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDNE 600

Query: 657 MSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDPVVIFNDETVIDS 716
           +S N LEITAI DN E+S+QPDDGE DPKKS VK KLQIQGKW+GVDPVV+F DETVIDS
Sbjct: 601 VSVNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFRDETVIDS 660

Query: 717 IKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQLKITSIGVKM 776
           IKTFYGI+E+FPL GHLVTRN+DTNHVKRIYY+SKSVKDVLELNF  GQQLKITSIG+KM
Sbjct: 661 IKTFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLKM 720

Query: 777 FERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSIKCADFVDAA 836
           FERQSS+EGS APC FRI+SEGLPVILPYITKQILTMSPVDFKHL++HKSIK ADF DAA
Sbjct: 721 FERQSSREGSCAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAA 780

Query: 837 FGEKASNLMLGCCVIVLGTG-NTASDVIGVDESTIAIGCWKGRASLSVMVTAIDCQELLG 896
           FGEKASNLMLGCCVIVLGTG NT+SDVI VDESTIAIGCWKGR+SLSVMVTAIDCQELL 
Sbjct: 781 FGEKASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLE 840

Query: 897 RLSNQLGNDL 903
           RLSN+LGND+
Sbjct: 841 RLSNRLGNDV 847

BLAST of Sed0018597 vs. NCBI nr
Match: KAG6604044.1 (RNA cytosine C(5)-methyltransferase NSUN2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034210.1 tRNA (cytosine(34)-C(5))-methyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1406.7 bits (3640), Expect = 0.0e+00
Identity = 713/850 (83.88%), Postives = 761/850 (89.53%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTHWEPFESQSQSPAFNE 116
           MG KGRG RARTQR HFRE R+NVWK SKPD NSAN   DGN  HW PF  QSQS AFNE
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPF--QSQSHAFNE 60

Query: 117 YYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVT--D 176
           YYKEQG+VTAEEWD FIEVLRK LPAAFRINSSGQFCGEIRAQLQNDF +SLQ+EVT  D
Sbjct: 61  YYKEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGD 120

Query: 177 EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 236
           EA AIRPL WY DNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL
Sbjct: 121 EAGAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 180

Query: 237 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIHQTK 296
           FLDVH NHYVLDMCAAPGSKTFQLLEIIHQSSK GDLP GLVVAND DVQRCNLLIHQTK
Sbjct: 181 FLDVHRNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTK 240

Query: 297 RMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGDGTL 356
           RMCT+NLIVTNHEAQHFPGCRA+TN FNAS AG EL  HNTQLTFDRVLCDVPCSGDGTL
Sbjct: 241 RMCTSNLIVTNHEAQHFPGCRAHTNFFNASAAGIELNHHNTQLTFDRVLCDVPCSGDGTL 300

Query: 357 RKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 416
           RKAPDIW+RWNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR
Sbjct: 301 RKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360

Query: 417 KCGGSLELVDVSNELPQLVRRPGLKKWKVLDRCVWLVTYKQVIERRQSMAIPSMFPSGRG 476
           K  GSLELVDVSNELPQLVRRPGLKKWKV DR VWL +YKQV + RQS+AIPSMFPSGRG
Sbjct: 361 KSEGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRG 420

Query: 477 CKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMRIVPHDQN 536
            KNQA  +HNSELR NHLDG+ GSSVD+SEPT + VHEFD EVCEFPIERCMRIVPHDQN
Sbjct: 421 RKNQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFD-EVCEFPIERCMRIVPHDQN 480

Query: 537 SGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISATDGMDRSP 596
           SGAFFIAVL+KVAPLP ATEKPS Q  K  N++ R KL Q IT+  +VD+SA+ GMD SP
Sbjct: 481 SGAFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESP 540

Query: 597 -EVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEVDLVVNG 656
            EV ID+NNQG  N ADNKP CE PVQDI+  +GLDSNAADG+DK++SE DLEV+LV N 
Sbjct: 541 SEVGIDSNNQGDFNSADNKPACEFPVQDIQGTEGLDSNAADGIDKQFSEADLEVNLVDNE 600

Query: 657 MSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDPVVIFNDETVIDS 716
           +S N LEITAI DN E+S+QPDDGE DPKKS VK KLQIQGKW+GVDPVV+F DETVIDS
Sbjct: 601 VSVNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDS 660

Query: 717 IKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQLKITSIGVKM 776
           IKTFYGI+E+FPL GHLVTRN+DTNHVKRIYY+SKSVKDVLELNF+ GQQLKITSIG+KM
Sbjct: 661 IKTFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFAVGQQLKITSIGLKM 720

Query: 777 FERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSIKCADFVDAA 836
           FERQSS+EGSSAPC FRI+SEGLPVILPYITKQILTMSPVDFKHL++HKSIK ADF DAA
Sbjct: 721 FERQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAA 780

Query: 837 FGEKASNLMLGCCVIVLGTG-NTASDVIGVDESTIAIGCWKGRASLSVMVTAIDCQELLG 896
           FGEKASNLMLGCCVIVLGTG NT+SDVI VDESTIAIGCWKGR+SLSVMVTAIDCQELL 
Sbjct: 781 FGEKASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLE 840

Query: 897 RLSNQLGNDL 903
           RLSN+LGND+
Sbjct: 841 RLSNRLGNDV 847

BLAST of Sed0018597 vs. ExPASy Swiss-Prot
Match: Q5ZLV4 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Gallus gallus OX=9031 GN=NSUN2 PE=2 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 4.6e-113
Identity = 286/830 (34.46%), Postives = 413/830 (49.76%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTHWEPFESQSQSPAFNE 116
           MGR+ R  R + Q +  RE                 G +D  G      E   ++  F  
Sbjct: 1   MGRRARDRRRQLQPQQRRE----------RSGGGGGGGDDQAGWAGGYPEIVKENELFER 60

Query: 117 YYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVTD-- 176
           YY+EQ +V   EWDAF+  LR+PLPA  RI        EI   L+  + + LQ    D  
Sbjct: 61  YYREQRIVPDGEWDAFMAALREPLPATLRITGYKSHAREILHCLKEKYFRELQHLEVDGQ 120

Query: 177 EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 236
           + E  + L WY + LAWH+N SR  LRK+  LERFH+FL  E E GNI+RQEAVSM+PPL
Sbjct: 121 KVEMPQALSWYPEELAWHTNLSRKILRKSPQLERFHQFLVSETECGNISRQEAVSMIPPL 180

Query: 237 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIHQTK 296
            L+V+P+H +LDMCAAPGSKT QL+E++H        P+G V+AND+D +RC LL+HQ K
Sbjct: 181 LLNVNPDHKILDMCAAPGSKTAQLIEMLHADMNV-PFPKGFVIANDVDNKRCYLLVHQAK 240

Query: 297 RMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGDGTL 356
           R+ +  ++V NH+A   P  +             ++      L +DR+LCDVPCSGDGT+
Sbjct: 241 RLNSPCIMVVNHDASSIPNLQV------------DVDGRKETLFYDRILCDVPCSGDGTM 300

Query: 357 RKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 416
           RK  D+WK+W +     LHGLQ++I  RG   L  GGRMVYSTCS+NP+ENEAV+A LL 
Sbjct: 301 RKNIDVWKKWTTQNSLQLHGLQLRIATRGVEQLAEGGRMVYSTCSLNPIENEAVIASLLE 360

Query: 417 KCGGSLELVDVSNELPQLVRRPGLKKWKV-LDRCVWLVTYKQVIERRQSMAIPSMFPSGR 476
           K  G+LEL DVS+ELP L R PG+ KWKV L    W   +K V   RQ+   P+MFP   
Sbjct: 361 KSQGALELADVSSELPGLKRMPGITKWKVMLKDGQWFEEWKDVPSNRQTQIRPTMFP--- 420

Query: 477 GCKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMRIVPHDQ 536
                                       I E         +E++    +ERC+RI+PH Q
Sbjct: 421 ----------------------------IKE---------EEKLKAMNLERCIRILPHHQ 480

Query: 537 NSGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISATDGMDRS 596
           N+G FF+AVL K +P+      P N++    + +  GK        E  +++AT+  D  
Sbjct: 481 NTGGFFVAVLIKKSPM------PWNKRQPKVHQKLPGK-------TEDTEVTATNAGD-- 540

Query: 597 PEVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEVDLVVNG 656
                      GS  A  KP         EDE+           KK  E           
Sbjct: 541 -----------GSEDATEKPTL------AEDEE----------PKKVQEL---------- 600

Query: 657 MSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDPVVIF-NDETVID 716
                         + D+EQ   G   P  S    K+++ G     DP V    D+ +  
Sbjct: 601 -------------QNSDTEQSKKGVCGPPPSK---KMKLFGFKE--DPFVFLPEDDPLFL 660

Query: 717 SIKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQLKITSIGVK 776
            I+ FY +D SFP + +L+TR  +    +++Y +SK +++VL  N    +++K+ + G+K
Sbjct: 661 PIQKFYALDPSFPKM-NLLTRTQE-GKKRQLYMVSKELRNVLLNN---SEKMKVINTGIK 686

Query: 777 MFERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSIKCADFVDA 836
           ++ R S  +G    C FR++ EG+  + P+I  +I+ +   D K LL  ++   + F  +
Sbjct: 721 VWSRNS--DGEQFGCAFRLAQEGIYTLYPFIHARIVNVCIEDVKILLTQENPFLSKF-SS 686

Query: 837 AFGEKASNLMLGCCVIVLGTGNTASDVIGVDESTIAIGCWKGRASLSVMV 883
               K  ++ +G  V+         D +   +  I +  W+G+ SL   V
Sbjct: 781 ETQRKVKDMAMGSIVLKYEPDPEKPDDL---QCPIVLCGWQGKTSLRAFV 686

BLAST of Sed0018597 vs. ExPASy Swiss-Prot
Match: Q1HFZ0 (RNA cytosine C(5)-methyltransferase NSUN2 OS=Mus musculus OX=10090 GN=Nsun2 PE=1 SV=2)

HSP 1 Score: 407.5 bits (1046), Expect = 3.9e-112
Identity = 285/838 (34.01%), Postives = 418/838 (49.88%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTH----WEPF--ESQSQ 116
           MGR+ RG R +                  P P      +DG        WE    E   +
Sbjct: 1   MGRRARGRRFQ----------------QPPQPEGEEDASDGGRKRGQAGWEGGYPEIVKE 60

Query: 117 SPAFNEYYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQA 176
           +  F  YY+E  +V   EWD F+E LR+PLPA  RI        EI   L+N + + L+ 
Sbjct: 61  NKLFEHYYQELKIVPEGEWDQFMESLREPLPATLRITGYKSHAKEILHCLKNKYFKELED 120

Query: 177 EVTD--EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAV 236
              D  + E  +PL WY + LAWH+N SR  LRK+  L +FH+FL  E E GNI+RQEAV
Sbjct: 121 LEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPLLAKFHQFLVSETESGNISRQEAV 180

Query: 237 SMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNL 296
           SM+PPL L+V P+H +LDMCAAPGSKT QL+E++H +  +   PEG V+AND+D +RC L
Sbjct: 181 SMIPPLLLNVEPHHKILDMCAAPGSKTTQLIEMLH-ADMSVPFPEGFVIANDVDNKRCYL 240

Query: 297 LIHQTKRMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPC 356
           L+HQ KR+ +  ++V NH+A   P                ++      L +DR+LCDVPC
Sbjct: 241 LVHQAKRLSSPCIMVVNHDASSIPRLTV------------DVDGRKEILFYDRILCDVPC 300

Query: 357 SGDGTLRKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAV 416
           SGDGT+RK  D+WK+W +     LHGLQ++I  RGA  L  GGRMVYSTCS+NPVE+EAV
Sbjct: 301 SGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPVEDEAV 360

Query: 417 VAELLRKCGGSLELVDVSNELPQLVRRPGLKKWKVLDR-CVWLVTYKQVIERRQSMAIPS 476
           +A LL K  G+LEL DVS ELP L   PG+ +WKV+ R   W   + +V + R +   P+
Sbjct: 361 IAALLEKSEGALELADVSAELPGLKWMPGVSQWKVMTRDGQWFADWHEVPQGRHTQIRPT 420

Query: 477 MFPSGRGCKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMR 536
           MFP                                  PT        E++    +ERC+R
Sbjct: 421 MFP----------------------------------PTDL------EKLQAMHLERCLR 480

Query: 537 IVPHDQNSGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISAT 596
           I+PH QN+G FF+AVL K AP+P    +P  Q +KSA         +A     S  ++AT
Sbjct: 481 ILPHHQNTGGFFVAVLVKKAPMPWNKRQPKVQ-NKSA---------EAREPRVSSHVAAT 540

Query: 597 DGMDRSPEVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEV 656
           +G                                         N +D       +++LE 
Sbjct: 541 EG-----------------------------------------NPSD-------QSELES 600

Query: 657 DLVVNGMSENKLEITAITDNSEDSEQPDD--GERDPKKSNVKGKLQIQGKWRGVDP-VVI 716
            ++         E    T+N+E +E+ D   G    KK  + G  +        DP V I
Sbjct: 601 QMITGA---GDSETAHNTENTESNEKKDGVCGPPPSKKMKLFGFKE--------DPFVFI 660

Query: 717 FNDETVIDSIKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQL 776
             D+ +   I+ FY +D SFP + +L+TR ++    +++Y +SK +++VL  N    +++
Sbjct: 661 PEDDPLFPPIEKFYALDPSFPRM-NLLTRTTE-GKKRQLYMVSKELRNVLLNN---SEKM 689

Query: 777 KITSIGVKMFERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSI 836
           K+ + G+K++ R +S  G    C FR++ EG+  + P+I  +I+T+S  D K LL  ++ 
Sbjct: 721 KVINTGIKVWCRNNS--GEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKTLLTQENP 689

Query: 837 KCADFVDAAFGEKASNLMLGCCVIVLGTGNTASDVIGVDESTIAIGCWKGRASLSVMV 883
                   A+ +   +L  G  V+     +   D +   +  I +  W+G+AS+   V
Sbjct: 781 FFRKLSSEAYSQ-VKDLAKGSVVLKYEPDSANPDTL---QCPIVLCGWRGKASIRTFV 689

BLAST of Sed0018597 vs. ExPASy Swiss-Prot
Match: Q08J23 (RNA cytosine C(5)-methyltransferase NSUN2 OS=Homo sapiens OX=9606 GN=NSUN2 PE=1 SV=2)

HSP 1 Score: 406.8 bits (1044), Expect = 6.6e-112
Identity = 285/836 (34.09%), Postives = 413/836 (49.40%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTH----WEPF--ESQSQ 116
           MGR+ RG R + Q+R                P  A    +G G      WE    E   +
Sbjct: 1   MGRRSRGRRLQQQQR----------------PEDAEDGAEGGGKRGEAGWEGGYPEIVKE 60

Query: 117 SPAFNEYYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQA 176
           +  F  YY+E  +V   EW  F++ LR+PLPA  RI        EI   L+N + + L+ 
Sbjct: 61  NKLFEHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFKELED 120

Query: 177 EVTD--EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAV 236
              D  + E  +PL WY + LAWH+N SR  LRK+  LE+FH+FL  E E GNI+RQEAV
Sbjct: 121 LEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAV 180

Query: 237 SMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNL 296
           SM+PPL L+V P+H +LDMCAAPGSKT QL+E++H        PEG V+AND+D +RC L
Sbjct: 181 SMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNV-PFPEGFVIANDVDNKRCYL 240

Query: 297 LIHQTKRMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPC 356
           L+HQ KR+ +  ++V NH+A   P  +             ++      L +DR+LCDVPC
Sbjct: 241 LVHQAKRLSSPCIMVVNHDASSIPRLQI------------DVDGRKEILFYDRILCDVPC 300

Query: 357 SGDGTLRKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAV 416
           SGDGT+RK  D+WK+W +     LHGLQ++I  RGA  L  GGRMVYSTCS+NP+E+EAV
Sbjct: 301 SGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAV 360

Query: 417 VAELLRKCGGSLELVDVSNELPQLVRRPGLKKWKVLDR-CVWLVTYKQVIERRQSMAIPS 476
           +A LL K  G+LEL DVSNELP L   PG+ +WKV+ +   W   +  V   R +   P+
Sbjct: 361 IASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPT 420

Query: 477 MFPSGRGCKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMR 536
           MFP               +L+  HL                              ERC+R
Sbjct: 421 MFP----------PKDPEKLQAMHL------------------------------ERCLR 480

Query: 537 IVPHDQNSGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISAT 596
           I+PH QN+G FF+AVL K + +P    +P  Q  KSA  R            ES  +S  
Sbjct: 481 ILPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQ-GKSAETR------------ESTQLSPA 540

Query: 597 DGMDRSPEVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEV 656
           D                     + KP          D   L+S +  G            
Sbjct: 541 D-------------------LTEGKP---------TDPSKLESPSFTG------------ 600

Query: 657 DLVVNGMSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDP-VVIFN 716
                       EI   T++ E++    DG   P  S    K+++ G     DP V I  
Sbjct: 601 --------TGDTEIAHATEDLENNGSKKDGVCGPPPSK---KMKLFGFKE--DPFVFIPE 660

Query: 717 DETVIDSIKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQLKI 776
           D+ +   I+ FY +D SFP + +L+TR ++    +++Y +SK +++VL  N    +++K+
Sbjct: 661 DDPLFPPIEKFYALDPSFPRM-NLLTRTTE-GKKRQLYMVSKELRNVLLNN---SEKMKV 690

Query: 777 TSIGVKMFERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSIKC 836
            + G+K++ R +S  G    C FR++ EG+  + P+I  +I+T+S  D K LL  ++   
Sbjct: 721 INTGIKVWCRNNS--GEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFF 690

Query: 837 ADFVDAAFGEKASNLMLGCCVIVLGTGNTASDVIGVDESTIAIGCWKGRASLSVMV 883
                  + + A +L  G  V+     +   D +   +  I +  W+G+AS+   V
Sbjct: 781 RKLSSETYSQ-AKDLAKGSIVLKYEPDSANPDAL---QCPIVLCGWRGKASIRTFV 690

BLAST of Sed0018597 vs. ExPASy Swiss-Prot
Match: Q28E61 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Xenopus tropicalis OX=8364 GN=nsun2 PE=2 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 4.4e-108
Identity = 273/838 (32.58%), Postives = 411/838 (49.05%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTHWE---PFESQSQSPA 116
           MGR+ R +R R QR   +       +  K    +A          WE   P E   ++  
Sbjct: 1   MGRRNRRNRQRHQRSTEQRSPAEEEQRRKAREQAA----------WECGYP-EIIKENKL 60

Query: 117 FNEYYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVT 176
           F  YY+E  +V   EWD F+  LR+PLPA  RI        EI   L+  + + LQ    
Sbjct: 61  FEHYYQELKIVPDGEWDKFMASLREPLPATIRITGYKSHAKEILHCLKEKYFKELQDIEV 120

Query: 177 D--EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMV 236
           D  + EA +PL WY + LAWH+N SR  +RK+  LE+FH+FL  E E GNI+RQEAVSM+
Sbjct: 121 DGQKIEAPQPLSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVNETESGNISRQEAVSMI 180

Query: 237 PPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIH 296
           PP+ L V P+H +LDMCAAPGSKT Q++E++H        PEG V+AND+D +RC LL+H
Sbjct: 181 PPVLLKVQPHHKILDMCAAPGSKTAQIIEMLHADMNV-PFPEGFVIANDVDNKRCYLLVH 240

Query: 297 QTKRMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGD 356
           Q KR+ +  ++V NH+A   P      N         E+      L +DR+LCDVPCSGD
Sbjct: 241 QAKRLNSPCIMVVNHDASSIPRLLVENN------GSREV------LYYDRILCDVPCSGD 300

Query: 357 GTLRKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAE 416
           GTLRK  D+WK+W +     LHGLQ++I  RG   L  GGRMVYSTCS+NPVE+EAV+A 
Sbjct: 301 GTLRKNIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIAS 360

Query: 417 LLRKCGGSLELVDVSNELPQLVRRPGLKKWKVLDR-CVWLVTYKQVIERRQSMAIPSMFP 476
           LL K  GSLEL DV++E+P L   PG+ +WKV+ +   W   ++ +   R +   P+MFP
Sbjct: 361 LLDKSEGSLELADVASEIPGLKWMPGITQWKVMTKEGHWYEKWEDIPTSRHTQIRPTMFP 420

Query: 477 SGRGCKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMRIVP 536
                                                    + +E++    + RCMRI+P
Sbjct: 421 P----------------------------------------KDEEKLKSMNLNRCMRILP 480

Query: 537 HDQNSGAFFIAVLQKVAPLPVATEKPSNQK-----SKSANNRYRGKLEQAITDAESVDIS 596
           H QN+G FF+AVL K AP+P    +P  Q+     +  A+     +L +A+T+  +    
Sbjct: 481 HHQNTGGFFVAVLIKKAPMPWNKRQPKLQRRPPVSACDASIAVAPELVKAVTENSA---- 540

Query: 597 ATDGMDRSPEVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDL 656
              GM   P VD +              PC              +N +D           
Sbjct: 541 ---GMADEPAVDTENG---------ETKPC--------------TNQSDSS--------- 600

Query: 657 EVDLVVNGMSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDP-VVI 716
           + D+V       K+++    +                                 DP V +
Sbjct: 601 KTDIVCCPPPSKKMKLFGFKE---------------------------------DPFVFV 660

Query: 717 FNDETVIDSIKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQL 776
             D+ + D I+TFY +D SFP   +L+TR  +    +++Y +SK +++VL  N    +++
Sbjct: 661 SEDDPIFDPIQTFYALDPSFPK-KNLLTRTQE-GKKRQLYMVSKELRNVLLHN---SEKM 691

Query: 777 KITSIGVKMFERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSI 836
           K+ + G+K+  R  + +G    C +R++ EG+  + P+I  +I+T+S  D K LL  ++ 
Sbjct: 721 KVINTGIKVLCR--NNDGEQYGCAYRLAQEGIYTLYPFINARIVTVSIEDIKVLLTQENP 691

Query: 837 KCADFVDAAFGEKASNLMLGCCVIVLGTGNTASDVIGVDESTIAIGCWKGRASLSVMV 883
             + F      ++A+N  +G  V+         + +   +  I +  W+G+ S+   V
Sbjct: 781 FLSKF-SKETQKQANNFDMGSIVLKYEPDPQEPETL---QCPIVLCGWRGKTSIRSFV 691

BLAST of Sed0018597 vs. ExPASy Swiss-Prot
Match: Q4V7N2 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Xenopus laevis OX=8355 GN=nsun2 PE=2 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 1.0e-104
Identity = 270/830 (32.53%), Postives = 407/830 (49.04%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTHWEPFESQSQSPAFNE 116
           MGRK R  R R +R   R   +   + ++       G+           E   ++  F  
Sbjct: 1   MGRKNR--RNRQRRTEQRSPAEEERRKAREQAAWEGGYP----------EIIKENKLFEH 60

Query: 117 YYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVTD-- 176
           YY+E  +V   EWD F++ LR+PLPA  RI        EI   L+  + + L     D  
Sbjct: 61  YYQELKIVPDGEWDKFMDALREPLPATIRITGYKSHAKEILHCLKEKYFKELPDIEVDGQ 120

Query: 177 EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 236
           + EA +PL WY + LAWH+N SR  +RK+  LE+FH+FL  E E GNI+RQEAVSM+PP+
Sbjct: 121 KIEAPQPLSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVSETESGNISRQEAVSMIPPV 180

Query: 237 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIHQTK 296
            L+V P+H +LDMCAAPGSKT Q++E++H        PEG V+AND+D +RC LL+HQ K
Sbjct: 181 LLNVQPHHKILDMCAAPGSKTAQIIEMLHADMNV-PFPEGFVIANDVDNKRCYLLVHQAK 240

Query: 297 RMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGDGTL 356
           R+ +  ++V NH+A   P      N         E+      L +DR+LCDVPCSGDGT+
Sbjct: 241 RLNSPCIMVVNHDASSIPRLLIENN------GSREV------LYYDRILCDVPCSGDGTM 300

Query: 357 RKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 416
           RK  D+WK+W +     LHGLQ++I  RG   L  GGRMVYSTCS+NPVE+EAV+  LL 
Sbjct: 301 RKNIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIVSLLD 360

Query: 417 KCGGSLELVDVSNELPQLVRRPGLKKWKVLDR-CVWLVTYKQVIERRQSMAIPSMFPSGR 476
           K  GSLEL DV++ELP L   PG+ +W+V+ +   W   ++ V   R +   P+MFP   
Sbjct: 361 KSEGSLELADVASELPGLKWMPGITQWRVMTKEGQWFEKWEDVPTSRHTQIRPTMFPL-- 420

Query: 477 GCKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMRIVPHDQ 536
                                                 + +E++    + RCMRI+PH Q
Sbjct: 421 --------------------------------------KDEEKLKSMNLNRCMRILPHHQ 480

Query: 537 NSGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISATDGMDRS 596
           N+G FF+AVL K AP+P    +P  Q+              ++ DA             +
Sbjct: 481 NTGGFFVAVLIKKAPMPWNKRQPKLQRRPPV----------SVCDASV-----------A 540

Query: 597 PEVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEVDLVVNG 656
           PE+                      +  I DE  +D+                     NG
Sbjct: 541 PEI----------------VKAVADISAIADEPAVDAE--------------------NG 600

Query: 657 MSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDPVVIFN-DETVID 716
                 E    T+ S  S+        P K     K+++ G     DP V  + D+ + +
Sbjct: 601 ------ETKPCTNQSGSSKTDSVCCPPPSK-----KMKLFGFKE--DPFVFLSEDDPIFE 660

Query: 717 SIKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQLKITSIGVK 776
            I+ FY +D SFP   +L+TR  +    +++Y +SK +++VL  N    +++K+ + G+K
Sbjct: 661 PIQKFYALDPSFPK-KNLLTRTQE-GKKRQLYMVSKELRNVLLHN---SEKMKVINTGIK 684

Query: 777 MFERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSIKCADFVDA 836
           +  R  + +G    C +R++ EG+  + P+I  +ILT+S  D K LL  ++   + F   
Sbjct: 721 VLCR--NNDGEQYGCAYRLAQEGIYSLYPFINARILTVSVEDIKVLLTQENPFLSKF-SK 684

Query: 837 AFGEKASNLMLGCCVIVLGTGNTASDVIGVDESTIAIGCWKGRASLSVMV 883
              ++A+NL +G  V+         + +   +  I +  W+G+ S+   V
Sbjct: 781 ETQKQANNLDMGSIVLKYEPDPQQPETL---QCPIVLCGWRGKTSIRSFV 684

BLAST of Sed0018597 vs. ExPASy TrEMBL
Match: A0A6J1HGN2 (tRNA (Cytosine(34)-C(5))-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463320 PE=3 SV=1)

HSP 1 Score: 1419.1 bits (3672), Expect = 0.0e+00
Identity = 719/849 (84.69%), Postives = 767/849 (90.34%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTHWEPFESQSQSPAFNE 116
           MG KGRG RARTQR+HFRE R+NVWK  KPDPNSANG  DGNG HWEPFESQS   AFNE
Sbjct: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSH--AFNE 60

Query: 117 YYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVT--D 176
           YYKEQG+VTAEEWD FIE LRKPLPAAFRINSSGQFCGEIRAQLQNDF +SLQAEVT  D
Sbjct: 61  YYKEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGD 120

Query: 177 EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 236
           EAEAIRPLPWY DNLAWHSNFSRMQLRKNQALE FHEFLKLENEIGNITRQEAVSMVPPL
Sbjct: 121 EAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPL 180

Query: 237 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIHQTK 296
           FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSK GDLP+GLVVANDLDVQRCNLLIHQTK
Sbjct: 181 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTK 240

Query: 297 RMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGDGTL 356
           RMCTANLIVTNHEAQHFPGCRANTN F+AS AGNELK  +TQLTFDRVLCDVPCSGDGTL
Sbjct: 241 RMCTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTL 300

Query: 357 RKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 416
           RKAPDIW++WNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR
Sbjct: 301 RKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360

Query: 417 KCGGSLELVDVSNELPQLVRRPGLKKWKVLDRCVWLVTYKQVIERRQSMAIPSMFPSGRG 476
           K GGSLELVDVSNELPQLVRRPGLKKWKV D+ VW  +Y+QV E  QS+AIP MFPSGRG
Sbjct: 361 KSGGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRG 420

Query: 477 CKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMRIVPHDQN 536
            K+   D HNSELR NH DG  GSSVD+SEPT + VH FDEEVCEFPIERCMRIVPHDQN
Sbjct: 421 RKS---DTHNSELRGNHPDG--GSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQN 480

Query: 537 SGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISATDGM-DRS 596
           +GAFF+AVL+KVAPLP ATEK S+Q +KSAN++  GKL+Q ITDA+SVD++ATDGM + S
Sbjct: 481 TGAFFVAVLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESS 540

Query: 597 PEVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEVDLVVNG 656
            EV+ID NNQ  SN A+N PPC+LPVQD +D DGLDS   DGMDK++SE D+EV  V N 
Sbjct: 541 SEVEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLDS--TDGMDKQFSEADMEV--VENE 600

Query: 657 MSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDPVVIFNDETVIDS 716
            +ENKLEITAI +NSE+S+QP DGE DPKK N K KLQIQGKWRGVDPVV+F DETVID 
Sbjct: 601 TTENKLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDC 660

Query: 717 IKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQLKITSIGVKM 776
           IKTFYGI+ESFPLIGHLVTRNSDTNHVKRIYYIS SVKDVLELNFSAGQQLKITSIG+KM
Sbjct: 661 IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKM 720

Query: 777 FERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSIKCADFVDAA 836
           FERQSS+EGSSAPC FRISSEGLPV+LPYITKQILTMSPVDFKHLL++KSIK ADFVDAA
Sbjct: 721 FERQSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAA 780

Query: 837 FGEKASNLMLGCCVIVLGTGNTASDVIGVDESTIAIGCWKGRASLSVMVTAIDCQELLGR 896
           FGEKASNLMLGCCVIVLGTGNT+SDVI VDESTIAIGCWKGRASLSVMVTA DCQELLGR
Sbjct: 781 FGEKASNLMLGCCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGR 838

Query: 897 LSNQLGNDL 903
           LSN+LGNDL
Sbjct: 841 LSNRLGNDL 838

BLAST of Sed0018597 vs. ExPASy TrEMBL
Match: A0A6J1KLF5 (tRNA (Cytosine(34)-C(5))-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111496800 PE=3 SV=1)

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 720/849 (84.81%), Postives = 768/849 (90.46%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTHWEPFESQSQSPAFNE 116
           MG KGRG RARTQR+HFRE R+NVWK  KPDPNSAN   DGNG HWEPFE  SQSPAFNE
Sbjct: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANDNKDGNGNHWEPFE--SQSPAFNE 60

Query: 117 YYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVT--D 176
           YYKEQG+VTAEEWD FIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDF++SLQAEVT  D
Sbjct: 61  YYKEQGIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFSRSLQAEVTDGD 120

Query: 177 EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 236
           EAEAIRPLPWY DNLAWHSNFSRMQLRKNQALE FHEFLKLENEIGNITRQEAVSMVPPL
Sbjct: 121 EAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPL 180

Query: 237 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIHQTK 296
           FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSK GDLP+GLVVANDLDVQRCNLLIHQTK
Sbjct: 181 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTK 240

Query: 297 RMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGDGTL 356
           RMCTANLIVTNHEAQHFPGCRANTN F+AS AGNELK  +TQLTFDRVLCDVPCSGDGTL
Sbjct: 241 RMCTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTL 300

Query: 357 RKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 416
           RKAPDIW++WNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR
Sbjct: 301 RKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360

Query: 417 KCGGSLELVDVSNELPQLVRRPGLKKWKVLDRCVWLVTYKQVIERRQSMAIPSMFPSGRG 476
           K GGSLELVDVSNELPQLVRRPGLKKWKV D+ VW  +Y+QV E  QS+AIP MFPSGRG
Sbjct: 361 KSGGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRG 420

Query: 477 CKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMRIVPHDQN 536
            K+   D HNS+LR NH DG  GSSVD+SEPT + VH FDEEVCEFPIERCMRIVPHDQN
Sbjct: 421 HKS---DTHNSQLRGNHPDG--GSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQN 480

Query: 537 SGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISATDGM-DRS 596
           +GAFF+AVL+KVAPL  ATEK S+Q +KSAN++ RGKL+Q ITDA+SVD++ATDGM + S
Sbjct: 481 TGAFFVAVLRKVAPLLAATEKTSDQNTKSANDQSRGKLQQTITDADSVDLNATDGMGESS 540

Query: 597 PEVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEVDLVVNG 656
            EV+ID NNQ  SN A+N PPC+LPVQD +D DGLDS   DGMDK +SE DLEV  V N 
Sbjct: 541 SEVEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLDS--TDGMDKPFSEADLEV--VDNE 600

Query: 657 MSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDPVVIFNDETVIDS 716
            +ENKLEITAI +NSE+S+QP DGE DPKK N K KLQIQGKWRGVDPVV+F DETVID 
Sbjct: 601 TTENKLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDC 660

Query: 717 IKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQLKITSIGVKM 776
           IKTFYGI+ESFPLIGHLVTRNSDTNHVKRIYYIS SVKDVLELNFSAGQQLKITSIG+KM
Sbjct: 661 IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKM 720

Query: 777 FERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSIKCADFVDAA 836
           FERQSS+EGSSAPC FRISSEGLPV+LPYITKQILTMSPVDFKHLL++KSIK ADFVDAA
Sbjct: 721 FERQSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAA 780

Query: 837 FGEKASNLMLGCCVIVLGTGNTASDVIGVDESTIAIGCWKGRASLSVMVTAIDCQELLGR 896
           FGEKASNLMLGCCVIVLGTGNT+SDVI VDESTIAIGCWKGRASLSVMVTA DCQELLGR
Sbjct: 781 FGEKASNLMLGCCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGR 838

Query: 897 LSNQLGNDL 903
           LSN+LGNDL
Sbjct: 841 LSNRLGNDL 838

BLAST of Sed0018597 vs. ExPASy TrEMBL
Match: A0A6J1GFP9 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453515 PE=3 SV=1)

HSP 1 Score: 1408.3 bits (3644), Expect = 0.0e+00
Identity = 712/850 (83.76%), Postives = 761/850 (89.53%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTHWEPFESQSQSPAFNE 116
           MG KGRG RARTQR HFRE R+NVWK SKPD NSAN   DGN  HW PF  QSQS AFNE
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPF--QSQSHAFNE 60

Query: 117 YYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVT--D 176
           YYKEQG+VTA EWD FIEVLRK LPAAFRINSSGQFCGEIRAQLQNDF +SLQ+EVT  D
Sbjct: 61  YYKEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGD 120

Query: 177 EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 236
           EA AIRPL WY DNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL
Sbjct: 121 EAGAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 180

Query: 237 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIHQTK 296
           FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSK GDLP GLVVAND DVQRCNLLIHQTK
Sbjct: 181 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTK 240

Query: 297 RMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGDGTL 356
           RMCT+NLIVTNHEAQHFPGCRA+TN+FNAS AG+EL  HNTQLTFDRVLCDVPCSGDGTL
Sbjct: 241 RMCTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDGTL 300

Query: 357 RKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 416
           RKAPDIW+RWNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR
Sbjct: 301 RKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360

Query: 417 KCGGSLELVDVSNELPQLVRRPGLKKWKVLDRCVWLVTYKQVIERRQSMAIPSMFPSGRG 476
           K  GSLELVDVSNELPQLVRRPGLKKWKV DR VWL +YKQV + RQS+AIPSMFPSGRG
Sbjct: 361 KSEGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRG 420

Query: 477 CKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMRIVPHDQN 536
            KNQA  +HNSELR NHLDG+ GSSVD+SEPT + VHEFD EVCEFPIERCMRIVPHDQN
Sbjct: 421 RKNQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFD-EVCEFPIERCMRIVPHDQN 480

Query: 537 SGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISATDGMDRSP 596
           SGAFFIAVL+KVAPLP ATEKPS Q  K  N++ R KL Q IT+  +VD+SA+ GMD SP
Sbjct: 481 SGAFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESP 540

Query: 597 -EVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEVDLVVNG 656
            EV +D+NNQG  N ADNKP CE PVQDIE  +GLDSNAADG+DK++SE DLEV+LV N 
Sbjct: 541 SEVGVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDNE 600

Query: 657 MSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDPVVIFNDETVIDS 716
           +S N LEITAI DN E+S+QPDDGE DPKKS VK KLQIQGKW+GVDPVV+F DETVIDS
Sbjct: 601 VSVNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFRDETVIDS 660

Query: 717 IKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQLKITSIGVKM 776
           IKTFYGI+E+FPL GHLVTRN+DTNHVKRIYY+SKSVKDVLELNF  GQQLKITSIG+KM
Sbjct: 661 IKTFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLKM 720

Query: 777 FERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSIKCADFVDAA 836
           FERQSS+EGS APC FRI+SEGLPVILPYITKQILTMSPVDFKHL++HKSIK ADF DAA
Sbjct: 721 FERQSSREGSCAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAA 780

Query: 837 FGEKASNLMLGCCVIVLGTG-NTASDVIGVDESTIAIGCWKGRASLSVMVTAIDCQELLG 896
           FGEKASNLMLGCCVIVLGTG NT+SDVI VDESTIAIGCWKGR+SLSVMVTAIDCQELL 
Sbjct: 781 FGEKASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLE 840

Query: 897 RLSNQLGNDL 903
           RLSN+LGND+
Sbjct: 841 RLSNRLGNDV 847

BLAST of Sed0018597 vs. ExPASy TrEMBL
Match: A0A6J1IQ20 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478413 PE=3 SV=1)

HSP 1 Score: 1406.0 bits (3638), Expect = 0.0e+00
Identity = 710/850 (83.53%), Postives = 764/850 (89.88%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTHWEPFESQSQSPAFNE 116
           MG KGRG RARTQR HFRE R+NVWK SKPD NSAN   DGN  HW PF  QSQS AFNE
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPF--QSQSHAFNE 60

Query: 117 YYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVT--D 176
           YYKEQG+VTAEEWD FIEVLRK LPAAFRINSSGQFCGEIRAQLQNDF +SLQ+EVT  D
Sbjct: 61  YYKEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGD 120

Query: 177 EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 236
           EA AIRPL WY DNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL
Sbjct: 121 EAGAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 180

Query: 237 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIHQTK 296
           FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSK GDLP GLVVAND DVQRCNLLIHQTK
Sbjct: 181 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTK 240

Query: 297 RMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGDGTL 356
           RMCT+NLIVTNHEAQHFPGCRA+TN FNAS AG+EL +HNTQLTFDRVLCDVPCSGDGTL
Sbjct: 241 RMCTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTL 300

Query: 357 RKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 416
           RKAPDIW+RWNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR
Sbjct: 301 RKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360

Query: 417 KCGGSLELVDVSNELPQLVRRPGLKKWKVLDRCVWLVTYKQVIERRQSMAIPSMFPSGRG 476
           K  GSLE+VDVSNELPQLVRRPGLKKWKV DR VWL +YKQV + RQS+AIPSMFP GRG
Sbjct: 361 KSEGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRG 420

Query: 477 CKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMRIVPHDQN 536
            KNQA  +HNSELR NHL+G+ GSSVD+SEPT + VHEFD EVCEFPIERCMRIVPHDQN
Sbjct: 421 RKNQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFD-EVCEFPIERCMRIVPHDQN 480

Query: 537 SGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISATDGMDRSP 596
           SGAFFIAVL+KVAPLP ATEKPS    K AN++ R KL Q IT+  +VD+SA+ GMD SP
Sbjct: 481 SGAFFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESP 540

Query: 597 -EVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEVDLVVNG 656
            EV ID+NNQG  N ADNKP CE PVQDIE ++GLDSNAADG+DK++SE DLEV+L+ N 
Sbjct: 541 SEVGIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNE 600

Query: 657 MSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDPVVIFNDETVIDS 716
           ++ N LEITAI DN E+S+QPDDGE DPKKS VK KLQIQGKW+GVDPVV+F DETVIDS
Sbjct: 601 VNVNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDS 660

Query: 717 IKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQLKITSIGVKM 776
           IKTFYGI+E+FPL GHLVTRN+DTNHVKRIYY+SKSVKDVLELNFS GQQLKITSIG+KM
Sbjct: 661 IKTFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKM 720

Query: 777 FERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSIKCADFVDAA 836
           FERQSS+EGSSAPC FRI+SEGLPVILPYITKQILTMSPVDFKHL++HKSIK ADF DAA
Sbjct: 721 FERQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAA 780

Query: 837 FGEKASNLMLGCCVIVLGT-GNTASDVIGVDESTIAIGCWKGRASLSVMVTAIDCQELLG 896
           FGEKAS+LMLGCCVIVLGT GNT+SDVI VDESTIAIGCWKGR+SLSVMVTAIDCQELL 
Sbjct: 781 FGEKASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLE 840

Query: 897 RLSNQLGNDL 903
           RLSN+LGND+
Sbjct: 841 RLSNRLGNDV 847

BLAST of Sed0018597 vs. ExPASy TrEMBL
Match: A0A6J1GFM2 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453515 PE=3 SV=1)

HSP 1 Score: 1382.5 bits (3577), Expect = 0.0e+00
Identity = 703/850 (82.71%), Postives = 752/850 (88.47%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPNSANGFNDGNGTHWEPFESQSQSPAFNE 116
           MG KGRG RARTQR HFRE R+NVWK SKPD NSAN   DGN  HW PF  QSQS AFNE
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPF--QSQSHAFNE 60

Query: 117 YYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVT--D 176
           YYKEQG+VTA EWD FIEVLRK LPAAFRINSSGQFCGEIRAQLQNDF +SLQ+EVT  D
Sbjct: 61  YYKEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGD 120

Query: 177 EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 236
           EA AIRPL WY DNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL
Sbjct: 121 EAGAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 180

Query: 237 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIHQTK 296
           FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSK GDLP GLVVAND DVQRCNLLIHQTK
Sbjct: 181 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTK 240

Query: 297 RMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGDGTL 356
           RMCT+NLIVTNHEAQHFPGCRA+TN+FNAS AG+EL  HNTQLTFDRVLCDVPCSGDGTL
Sbjct: 241 RMCTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDGTL 300

Query: 357 RKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 416
           RKAPDIW+RWNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR
Sbjct: 301 RKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360

Query: 417 KCGGSLELVDVSNELPQLVRRPGLKKWKVLDRCVWLVTYKQVIERRQSMAIPSMFPSGRG 476
           K  GSLELVDVSNELPQLVRRPGLKKWKV DR VWL +YKQV + RQS+AIPSMFPSGRG
Sbjct: 361 KSEGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRG 420

Query: 477 CKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMRIVPHDQN 536
            KNQA  +HNSELR NHLDG+ GSSVD+SEPT + VHEFD EVCEFPIERCMRIVPHDQN
Sbjct: 421 RKNQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFD-EVCEFPIERCMRIVPHDQN 480

Query: 537 SGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISATDGMDRSP 596
           SGAFFIAVL+KVAPLP ATEKPS Q  K  N++ R KL Q IT+  +VD+SA+ GMD SP
Sbjct: 481 SGAFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESP 540

Query: 597 -EVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEVDLVVNG 656
            EV +D+NNQG  N ADNKP CE PVQDIE  +GLDSNAADG+DK++SE DLEV+LV N 
Sbjct: 541 SEVGVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDNE 600

Query: 657 MSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDPVVIFNDETVIDS 716
           +S N LEITAI DN E+S          K+S VK KLQIQGKW+GVDPVV+F DETVIDS
Sbjct: 601 VSVNNLEITAIEDNLEES----------KQSTVKRKLQIQGKWKGVDPVVLFRDETVIDS 660

Query: 717 IKTFYGIDESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAGQQLKITSIGVKM 776
           IKTFYGI+E+FPL GHLVTRN+DTNHVKRIYY+SKSVKDVLELNF  GQQLKITSIG+KM
Sbjct: 661 IKTFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLKM 720

Query: 777 FERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSIKCADFVDAA 836
           FERQSS+EGS APC FRI+SEGLPVILPYITKQILTMSPVDFKHL++HKSIK ADF DAA
Sbjct: 721 FERQSSREGSCAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAA 780

Query: 837 FGEKASNLMLGCCVIVLGTG-NTASDVIGVDESTIAIGCWKGRASLSVMVTAIDCQELLG 896
           FGEKASNLMLGCCVIVLGTG NT+SDVI VDESTIAIGCWKGR+SLSVMVTAIDCQELL 
Sbjct: 781 FGEKASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLE 837

Query: 897 RLSNQLGNDL 903
           RLSN+LGND+
Sbjct: 841 RLSNRLGNDV 837

BLAST of Sed0018597 vs. TAIR 10
Match: AT2G22400.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 885.2 bits (2286), Expect = 4.5e-257
Identity = 480/856 (56.07%), Postives = 593/856 (69.28%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGRDNVWKLSKPDPN---SANGFNDGNGTHWEPFESQSQSPA 116
           MGR GR  R RTQR+ F+E R+NVWK  K D +   S N   +   T WEP    + +P 
Sbjct: 1   MGRGGR-HRGRTQRKDFKESRENVWKRPKSDASVDGSDNAVPEQKPT-WEPI--VTVNPN 60

Query: 117 FNEYYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVT 176
           F EYYK+QG+V AEEWD F+E+LRKPLPAAFR+NS+GQFC EI + L+NDF +SLQAE  
Sbjct: 61  FEEYYKKQGIVKAEEWDLFMEILRKPLPAAFRVNSNGQFCDEIISILENDFMKSLQAEAI 120

Query: 177 D--EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMV 236
           +  E EAI+PLPWY  NLAWHSNFSR ++RKNQ LERFHEFLKLENE+GNITRQEAVSMV
Sbjct: 121 EGGELEAIKPLPWYPKNLAWHSNFSRKEIRKNQTLERFHEFLKLENEVGNITRQEAVSMV 180

Query: 237 PPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIH 296
           PPLFLDVHP+H+VLDMCAAPGSKTFQLLEIIH++S+ G LP GLVVAND+D +R NLLIH
Sbjct: 181 PPLFLDVHPDHFVLDMCAAPGSKTFQLLEIIHEASEPGSLPNGLVVANDVDFKRSNLLIH 240

Query: 297 QTKRMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGD 356
           QTKRMCT+NLIVTNHE Q FPGCR N +   AS  G        QL FDRVLCDVPCSGD
Sbjct: 241 QTKRMCTSNLIVTNHEGQQFPGCRLNKS--RASEKGISENMPINQLAFDRVLCDVPCSGD 300

Query: 357 GTLRKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAE 416
           GTLRKAPDIW++WNSGMGNGLH LQ+ + MRG SLLKVGG+M+YSTCSMNPVE+EAVVAE
Sbjct: 301 GTLRKAPDIWRKWNSGMGNGLHSLQIILAMRGLSLLKVGGKMIYSTCSMNPVEDEAVVAE 360

Query: 417 LLRKCGGSLELVDVSNELPQLVRRPGLKKWKVLDRCVWLVTYKQVIERRQSMAIPSMFPS 476
           +LR+CG S+EL+DVS++LP+L+RRPGLK WKV D+  W  +YK V + R+   + SMFPS
Sbjct: 361 ILRRCGDSVELLDVSDKLPELIRRPGLKAWKVRDKGGWFTSYKDVPQNRRGGVLVSMFPS 420

Query: 477 GRGC--------KNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIE 536
           G+          KN+ GD +  E      DG   + + + + T     E  EEV + P+E
Sbjct: 421 GKYLKDSTETTEKNENGDVNGCE------DGLKETDISVVDATP---EEQAEEVSDLPLE 480

Query: 537 RCMRIVPHDQNSGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVD 596
           RCMRI+PHDQN+GAFFIAVLQK +PLP   EKP+ +++ +A                   
Sbjct: 481 RCMRIIPHDQNTGAFFIAVLQKKSPLPEFQEKPNTKRNSTA------------------- 540

Query: 597 ISATDGMDRSPEVDIDTNNQGGSNFADNKPPCELPVQD-IEDEDGLDSNAADGMDKKYSE 656
             +TD  ++SP  +       G        P E  V+  IE +  ++ N +   +KK +E
Sbjct: 541 -KSTDSTEKSPSKESVVTVDAG-------VPDESAVEKVIEADSNIEKNDSLEPEKKITE 600

Query: 657 TDLEVDLVVNGMSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQIQGKWRGVDPV 716
            +                  +IT++ E +     G+R         K+ +QGKW+G DPV
Sbjct: 601 GE------------------SITEDKEANSSNAGGKR---------KVPMQGKWKGFDPV 660

Query: 717 VIFNDETVIDSIKTFYGI-DESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSAG 776
           V   DETVI+ IK FYGI DESFPL GHLV RN+DT+ VKRIYY+SKSVK+VL+LNF+ G
Sbjct: 661 VFVKDETVINGIKEFYGIKDESFPLHGHLVARNTDTSSVKRIYYVSKSVKEVLQLNFAVG 720

Query: 777 QQLKITSIGVKMFERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEH 836
           QQLKI S+G+KMFERQS+KEGSS  C FRISSEGLPVILPYITKQ+L     DFK LL+ 
Sbjct: 721 QQLKIASVGLKMFERQSAKEGSSTLCPFRISSEGLPVILPYITKQVLYTPMADFKLLLQD 780

Query: 837 KSIKCADFVDAAFGEKASNLMLGCCVIVLGTGNTASDVIGVDESTIAIGCWKGRASLSVM 896
           KSIK  DFV+    +KA++L++G CV++L  G      +  D STIAI CW+G+ASL+VM
Sbjct: 781 KSIKFHDFVNPQLAQKATDLVMGSCVVILSDGEVP---VKADASTIAISCWRGKASLAVM 784

Query: 897 VTAIDCQELLGRLSNQ 898
            T  DCQELL R + +
Sbjct: 841 ATVADCQELLERFAEK 784

BLAST of Sed0018597 vs. TAIR 10
Match: AT4G40000.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 812.8 bits (2098), Expect = 2.8e-235
Identity = 461/853 (54.04%), Postives = 568/853 (66.59%), Query Frame = 0

Query: 57  MGRKGRGSRARTQRRHFREGR--DNVWKLSKPDPNSANGFNDGNGTHWEPFESQSQSPAF 116
           MGR GR  R  T +R+  E    DNV K     P  +    D N    +P  S      F
Sbjct: 1   MGR-GRRHRGSTSKRNQIENSNDDNVRK-----PLKSEASFDSNNLQ-KPICSNLH---F 60

Query: 117 NEYYKEQGVVTAEEWDAFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFAQSLQAEVTD 176
            EYYKEQ +V A+EWD+F+E LRKPLPAAFR+ S+GQFC EIR +L+NDF  SLQAE  +
Sbjct: 61  EEYYKEQRIVKAQEWDSFMEFLRKPLPAAFRVYSNGQFCDEIRLKLENDFMISLQAEAIE 120

Query: 177 --EAEAIRPLPWYHDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP 236
             E EAI+PLPWY  NLAWHSNFSR ++RKNQ LERFHEFLKLE E GN+TRQE+VSMVP
Sbjct: 121 SGELEAIKPLPWYPKNLAWHSNFSRKEIRKNQTLERFHEFLKLETEAGNMTRQESVSMVP 180

Query: 237 PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCNLLIHQ 296
           PLFLDVHP+H+VLDMCAAPGSKTFQLLEIIH+SS+ G LP G+VVAND+D +R NLLIHQ
Sbjct: 181 PLFLDVHPDHFVLDMCAAPGSKTFQLLEIIHESSEPGSLPNGMVVANDVDYKRSNLLIHQ 240

Query: 297 TKRMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVPCSGDG 356
           TKR CT NL+VTN+E QHFP C     L   SVA         QL FDRVLCDVPCSGDG
Sbjct: 241 TKRTCTTNLMVTNNEGQHFPSCNTKRTL---SVASETNPHPIDQLLFDRVLCDVPCSGDG 300

Query: 357 TLRKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL 416
           TLRKAPDIW+RWNSG GNGLH LQV + MRG SLLKVGGRMVYSTCSMNP+E+EAVVAE+
Sbjct: 301 TLRKAPDIWRRWNSGSGNGLHSLQVVLAMRGLSLLKVGGRMVYSTCSMNPIEDEAVVAEI 360

Query: 417 LRKCGGSLELVDVSNELPQLVRRPGLKKWKVLDRCVWLVTYKQVIERRQSMAIPSMFPSG 476
           LR+CG S+ELVDVS++LP+L+RRPGL KWKV DR  W  +YK V + ++   + SMFPSG
Sbjct: 361 LRRCGCSVELVDVSDKLPELIRRPGLTKWKVHDRGGWYRSYKDVPKSQRDGVLRSMFPSG 420

Query: 477 RGCKNQAGDNHNSELRCNHLDGTTGSSVDISEPTTNSVHEFDEEVCEFPIERCMRIVPHD 536
           +  K+ +G  ++ E                 E  + S  E  EEVC+ P+E CMRI+PHD
Sbjct: 421 KSDKDSSGGKNSYE-----------------EMASISSDESAEEVCDLPLELCMRILPHD 480

Query: 537 QNSGAFFIAVLQKVAPLPVATEKPSNQKSKSANNRYRGKLEQAITDAESVDISATDGMDR 596
           QN+G FFIAVL KV+PLP   EK + +++ S  N      + +  +A S           
Sbjct: 481 QNTGGFFIAVLHKVSPLPDFQEKVTQKRNLSTRNNINSSEKSSSYEAVS----------- 540

Query: 597 SPEVDIDTNNQGGSNFADNKPPCELPVQDIEDEDGLDSNAADGMDKKYSETDLEVDLVVN 656
                 DT           KP           E+G +            E  LE  +  N
Sbjct: 541 ------DT--------VVTKP-----------EEGTE------------EIVLEATVSEN 600

Query: 657 GMSENKLEITAITDNSEDSEQPDDGERDPKKSNVKGKLQI---QGKWRGVDPVVIFNDET 716
           G    ++E T      E +++ DD         ++GK ++   QGKW+G+ PVV   D+T
Sbjct: 601 GFEPPEMESTWNEGIVELAQKLDD---------IEGKREVPSMQGKWKGLYPVVFLRDQT 660

Query: 717 VIDSIKTFYGI-DESFPLIGHLVTRNSDTN---HVKRIYYISKSVKDVLELNFSAGQQLK 776
            I+ IKTFYGI DESFPL GHLVTRNSD +   +VKRIYY+SK+VK+VLELNF+ G+ LK
Sbjct: 661 GINGIKTFYGIKDESFPLYGHLVTRNSDISSHGNVKRIYYVSKAVKNVLELNFAVGKPLK 720

Query: 777 ITSIGVKMFERQSSKEGSSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLEHKSIK 836
           I+S+G+KMFE+QS+KE  +  C FRI+SEGLPVILPY+TKQIL  +  DFK+LL+HKSIK
Sbjct: 721 ISSVGLKMFEKQSAKECEANCCSFRITSEGLPVILPYMTKQILYATMADFKNLLQHKSIK 766

Query: 837 CADFVDAAFGEKASNLMLGCCVIVL-GTGNTASDVIGVDESTIAIGCWKGRASLSVMVTA 896
             DF+    GEKA+ L LG CV+VL       S+   V+ STIAIGCWKG+ASL+VMVT 
Sbjct: 781 FLDFIHPQIGEKAAELALGSCVMVLVDDSQLGSEQFKVNSSTIAIGCWKGKASLTVMVTT 766

Query: 897 IDCQELLGRLSNQ 898
           +DC +L+ RLS++
Sbjct: 841 VDCHQLIQRLSDR 766

BLAST of Sed0018597 vs. TAIR 10
Match: AT5G55920.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 1.2e-15
Identity = 61/195 (31.28%), Postives = 86/195 (44.10%), Query Frame = 0

Query: 225 QEAVSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQ 284
           Q A S +P + L    N  ++D+ AAPG KT  +  ++  +        GL+ AN++ V 
Sbjct: 344 QGASSFLPVMALAPRENERIVDVAAAPGGKTTYIAALMKNT--------GLIYANEMKVP 403

Query: 285 RCNLLIHQTKRMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLC 344
           R   L     RM   N IV N++ +  P            V G        Q T DRVL 
Sbjct: 404 RLKSLTANLHRMGVTNTIVCNYDGRELP-----------KVLG--------QNTVDRVLL 463

Query: 345 DVPCSGDGTLRKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLL----KVGGRMVYSTCSM 404
           D PCSG G + K   +              LQ Q+ +    ++    K GG +VYSTCS+
Sbjct: 464 DAPCSGTGIISKDESVKITKTMDEIKKFAHLQKQLLLAAIDMVDANSKTGGYIVYSTCSI 511

Query: 405 NPVENEAVVAELLRK 416
              ENEAV+   L+K
Sbjct: 524 MVTENEAVIDYALKK 511

BLAST of Sed0018597 vs. TAIR 10
Match: AT4G26600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 1.7e-14
Identity = 60/194 (30.93%), Postives = 85/194 (43.81%), Query Frame = 0

Query: 225 QEAVSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQ 284
           Q A S +P + L       V+DM AAPG KT  +  ++  +        G++ AN++ V 
Sbjct: 327 QSASSFLPVMALAPREKERVVDMAAAPGGKTTYVAALMKNT--------GIIYANEMKVP 386

Query: 285 RCNLLIHQTKRMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLC 344
           R   L     RM   N IV N++                   G EL     Q + DRVL 
Sbjct: 387 RLKSLSANLHRMGVTNTIVCNYD-------------------GRELTKVLGQSSVDRVLL 446

Query: 345 DVPCSGDGTLRKAPDIWKRWNSGMGNGLHGLQVQIGMRGASLL----KVGGRMVYSTCSM 404
           D PCSG G + K   +    ++        LQ Q+ +    L+    K GG +VYSTCS+
Sbjct: 447 DAPCSGTGVISKDESVKTSKSADDIKKFAHLQKQLILGAIDLVDANSKTGGYIVYSTCSV 493

Query: 405 NPVENEAVVAELLR 415
              ENEAV+   L+
Sbjct: 507 MIPENEAVIDYALK 493

BLAST of Sed0018597 vs. TAIR 10
Match: AT3G13180.1 (NOL1/NOP2/sun family protein / antitermination NusB domain-containing protein )

HSP 1 Score: 76.6 bits (187), Expect = 1.1e-13
Identity = 61/190 (32.11%), Postives = 89/190 (46.84%), Query Frame = 0

Query: 228 VSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKAGDLPEGLVVANDLDVQRCN 287
           VS+V P      P   ++D CAAPG KT  +   +          +G++ A D++  R  
Sbjct: 323 VSVVKP-----QPGERIMDACAAPGGKTLFMASCLK--------GQGMIYAMDVNEGRLR 382

Query: 288 LLIHQTKRMCTANLIVTNHEAQHFPGCRANTNLFNASVAGNELKTHNTQLTFDRVLCDVP 347
           +L    K      LI T H         ++  +F              ++ +D+VL D P
Sbjct: 383 ILGETAKSHQVDGLITTIH---------SDLRVF----------AETNEVQYDKVLLDAP 442

Query: 348 CSGDGTLRKAPDIWKRWNSGMGNGLH--GLQVQIGMRGASLLKVGGRMVYSTCSMNPVEN 407
           CSG G L K  D+  RWN  + + L    LQ ++    + L+K GG +VYSTCS++P EN
Sbjct: 443 CSGLGVLSKRADL--RWNRKLEDMLELTKLQDELLDSASKLVKHGGVLVYSTCSIDPEEN 478

Query: 408 EA-VVAELLR 415
           E  V A LLR
Sbjct: 503 EGRVEAFLLR 478