Homology
BLAST of Sed0018375 vs. NCBI nr
Match:
XP_016900594.1 (PREDICTED: subtilisin-like protease SBT5.3, partial [Cucumis melo])
HSP 1 Score: 1297.7 bits (3357), Expect = 0.0e+00
Identity = 632/747 (84.61%), Postives = 694/747 (92.90%), Query Frame = 0
Query: 10 LLHLVQSYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHIN 69
+ +L+QSYIVYLGSHSHG +PS A L IA+ESHYSLLGS+LGSNE AKEAIFYSYNRHIN
Sbjct: 1 IANLLQSYIVYLGSHSHGSNPSAADLEIATESHYSLLGSLLGSNEAAKEAIFYSYNRHIN 60
Query: 70 GFAAILDQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLGVENDDGIPANSIWNLASFG 129
GFAAILDQ V EDLA++P V S++ENKGRKLHTTSSW+FLGVE+DDGIP NSIWN ASFG
Sbjct: 61 GFAAILDQKVVEDLARNPAVASIHENKGRKLHTTSSWKFLGVEHDDGIPHNSIWNRASFG 120
Query: 130 ESTIIANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKFHCNRKLIGARYFNKGYEAI 189
ESTII NLDTGVWPESKSF+DEGYGP+PT+WKGSCEGGSKFHCNRKLIGARYFNKGY A
Sbjct: 121 ESTIIGNLDTGVWPESKSFNDEGYGPVPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAY 180
Query: 190 VGSLNASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKARVVAYKVCWP 249
GSLNASYETARD++GHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKA V AYKVCWP
Sbjct: 181 AGSLNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKALVAAYKVCWP 240
Query: 250 QQSSGGGCFDADILAAIEAAISDGVDVLSLSLGGNSKEFLDDVTAIGAFHAFQNGIVVVC 309
Q SGGGCFDADILAAIEAAISDGVD+LSLSLGG +K+F +DVTAIGAFHA Q GI+VVC
Sbjct: 241 QVDSGGGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSEDVTAIGAFHAVQQGIIVVC 300
Query: 310 SAGNSGPALGTVENVAPWLVTVAASTINREFTNYLALGNQKHVKGASLSDKILPTQKFYP 369
SAGNSGPA GT+EN APW++TV ASTINR+FT+Y+ALGN+KH+KGASLSDKILP QKFYP
Sbjct: 301 SAGNSGPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKHIKGASLSDKILPEQKFYP 360
Query: 370 LINAADAKANNVSADDARLCQDGMLDPEEVKGKIIVCLRGENGRVDKGYAAAQAGAVGMI 429
LINAADAKANNVS+D A+LCQ G LDP++VKGKII+CLRGEN RVDKGYAAAQAGAVGMI
Sbjct: 361 LINAADAKANNVSSDVAQLCQAGSLDPKKVKGKIILCLRGENARVDKGYAAAQAGAVGMI 420
Query: 430 LANTEQNGDELMADAHLLPASHVSYTDGESIYQYINSTKTPMAYMTHVRTELGVKPAPFM 489
LAN EQNGDEL+ADAHLLP SHVSYTDG+SIYQYIN TKTPMAYMTHVRTELG+KPAP M
Sbjct: 421 LANAEQNGDELIADAHLLPVSHVSYTDGQSIYQYINFTKTPMAYMTHVRTELGIKPAPVM 480
Query: 490 AAFSSRGPNTIEESILKPDITAPGVNIMAAYSEDTSPSGSPFDNRRIPFNIISGTSMSCP 549
A+FSSRGPNTIEESILKPDITAPGVNI+AAYSED SPSGS FDNRRIPFNI+SGTSMSCP
Sbjct: 481 ASFSSRGPNTIEESILKPDITAPGVNILAAYSEDASPSGSLFDNRRIPFNIVSGTSMSCP 540
Query: 550 HISGIVGLLKTLYPQWSPAAIKSAIMTTAETRANDLHPILDTTKVKANPFAYGAGHVRPN 609
HISGIVGLLKTLYP WSPAAIKSAIMTTAETRANDLHPIL+T +KANPFAYGAGHV+PN
Sbjct: 541 HISGIVGLLKTLYPTWSPAAIKSAIMTTAETRANDLHPILNTVNLKANPFAYGAGHVQPN 600
Query: 610 RAVNPGLVYDLTTKDYLNFLCARGYNETKITKFSNTSFVCSKSFKLTDFNYPSISIPNLK 669
RA+NPGLVYDLTT DY+NFLCA+GYN+++I+KFS TSFVCSKSFKLTDFNYPSISIP++K
Sbjct: 601 RAMNPGLVYDLTTNDYMNFLCAQGYNKSQISKFSATSFVCSKSFKLTDFNYPSISIPDMK 660
Query: 670 SGPVTIKRTVKNVGSPSTYVARVKVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSK 729
SG VTI R VKNVG PSTYVARVKVP GVSVSV+P TL+FTGIDEEK+F+V + SVAN+K
Sbjct: 661 SGVVTINRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFTGIDEEKSFKVVIGSVANNK 720
Query: 730 NQGYVFGSLAWSDGKHYVRSPIVVNLG 757
++GYVFGSL W DGKH+VRSPIVVNLG
Sbjct: 721 HRGYVFGSLIWEDGKHHVRSPIVVNLG 747
BLAST of Sed0018375 vs. NCBI nr
Match:
XP_038904203.1 (uncharacterized protein LOC120090550 [Benincasa hispida])
HSP 1 Score: 1293.1 bits (3345), Expect = 0.0e+00
Identity = 641/765 (83.79%), Postives = 696/765 (90.98%), Query Frame = 0
Query: 1 MSFQLKTFILLHLVQ--------SYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGS 60
+S L F L L+Q SYIVYLGSHSHG +PS L+IA+ESHYSLLGS+LGS
Sbjct: 820 LSSLLLLFFLFPLLQTSTIATQKSYIVYLGSHSHGSNPSSVDLQIATESHYSLLGSLLGS 879
Query: 61 NEEAKEAIFYSYNRHINGFAAILDQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLGVE 120
NE AKEAIFYSYNRHINGFAA+LD VAEDLA++P V SV+ENKGRKLHTTSSW+FLGVE
Sbjct: 880 NEAAKEAIFYSYNRHINGFAAMLDHKVAEDLARNPAVASVHENKGRKLHTTSSWKFLGVE 939
Query: 121 NDDGIPANSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKFHC 180
+DDGIP SIWNLASFGESTIIANLDTGVWPESKSFSDEGYGP+PT+WKGSCEGGSKFHC
Sbjct: 940 HDDGIPTKSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPVPTRWKGSCEGGSKFHC 999
Query: 181 NRKLIGARYFNKGYEAIVGSLNASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNGTA 240
NRKLIGARYFNKGY A VGSLNA+YETARD DGHGTHTLSTAGGNFISGANVFGNGNGTA
Sbjct: 1000 NRKLIGARYFNKGYAAYVGSLNATYETARDHDGHGTHTLSTAGGNFISGANVFGNGNGTA 1059
Query: 241 KGGSPKARVVAYKVCWPQQSSGGGCFDADILAAIEAAISDGVDVLSLSLGGNSKEFLDDV 300
KGGSPKA V AYKVCWP SGG CFDADILAAIEAAISDGVDVLSLSLGG+SK+F DDV
Sbjct: 1060 KGGSPKALVAAYKVCWPPVDSGGSCFDADILAAIEAAISDGVDVLSLSLGGDSKDFSDDV 1119
Query: 301 TAIGAFHAFQNGIVVVCSAGNSGPALGTVENVAPWLVTVAASTINREFTNYLALGNQKHV 360
AIGAFHA Q GIVVVCSAGNSGPA GTVENVAPW+ TV ASTINREFT+Y+ALGN+KH+
Sbjct: 1120 AAIGAFHAVQQGIVVVCSAGNSGPAPGTVENVAPWIFTVGASTINREFTSYVALGNKKHI 1179
Query: 361 KGASLSDKILPTQKFYPLINAADAKANNVSADDARLCQDGMLDPEEVKGKIIVCLRGENG 420
KGASLSDKILP QKFYPLI+AA AKAN+VS DDA+LC++G LDP +VKGKII+CLRGEN
Sbjct: 1180 KGASLSDKILPAQKFYPLISAAYAKANHVSFDDAQLCEEGSLDPRKVKGKIIICLRGENA 1239
Query: 421 RVDKGYAAAQAGAVGMILANTEQNGDELMADAHLLPASHVSYTDGESIYQYINSTKTPMA 480
RVDKGY AAQAGAVGMILANTEQN DEL+ADAHLLP SHVSYTDG+SIYQYINSTKTPMA
Sbjct: 1240 RVDKGYVAAQAGAVGMILANTEQNEDELIADAHLLPVSHVSYTDGQSIYQYINSTKTPMA 1299
Query: 481 YMTHVRTELGVKPAPFMAAFSSRGPNTIEESILKPDITAPGVNIMAAYSEDTSPSGSPFD 540
YMTH RTELG+KPAP MA+FSSRGPNT+EESILKPDITAPGVNI+AAYSED SPSGS FD
Sbjct: 1300 YMTHARTELGIKPAPVMASFSSRGPNTMEESILKPDITAPGVNILAAYSEDASPSGSSFD 1359
Query: 541 NRRIPFNIISGTSMSCPHISGIVGLLKTLYPQWSPAAIKSAIMTTAETRANDLHPILDTT 600
NRRIPFNI+SGTSMSCPHISGIVGLLKTLYP+WSPAAIKSAIMTTAETRAND HPIL+T
Sbjct: 1360 NRRIPFNIVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANDKHPILNTE 1419
Query: 601 KVKANPFAYGAGHVRPNRAVNPGLVYDLTTKDYLNFLCARGYNETKITKFSNTSFVCSKS 660
++KANPFAYGAGHV+PNRA+NPGLVYDLTTKDYLNFLC GYN+T+I+KFSNTSFVCSKS
Sbjct: 1420 ELKANPFAYGAGHVQPNRAMNPGLVYDLTTKDYLNFLCVLGYNKTQISKFSNTSFVCSKS 1479
Query: 661 FKLTDFNYPSISIPNLKSGPVTIKRTVKNVGSPSTYVARVKVPPGVSVSVKPSTLEFTGI 720
FKLTDFNYPSISIPN+K G VTIKR VKNVG PSTYVARVKVPPG SVSVKPSTL+FTGI
Sbjct: 1480 FKLTDFNYPSISIPNMKPGLVTIKRRVKNVGKPSTYVARVKVPPGASVSVKPSTLKFTGI 1539
Query: 721 DEEKTFQVELQSVANSK-NQGYVFGSLAWSDGKHYVRSPIVVNLG 757
DEEK+F+V + SVA++K ++GYVFGSL W DGKH+VRSPIVVNLG
Sbjct: 1540 DEEKSFKVIVGSVADNKHSRGYVFGSLVWEDGKHHVRSPIVVNLG 1584
BLAST of Sed0018375 vs. NCBI nr
Match:
TYK19383.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])
HSP 1 Score: 1229.2 bits (3179), Expect = 0.0e+00
Identity = 604/744 (81.18%), Postives = 671/744 (90.19%), Query Frame = 0
Query: 13 LVQSYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHINGFA 72
+ +SYIVYLGSHSHG +PS A L IA+ESHYSLLGS+LGSNE AKEAIFYSYNRHINGFA
Sbjct: 1 MAKSYIVYLGSHSHGSNPSAADLEIATESHYSLLGSLLGSNEAAKEAIFYSYNRHINGFA 60
Query: 73 AILDQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLGVENDDGIPANSIWNLASFGEST 132
AILDQ V EDLA++P V S++ENKGRKLHTTSSW+FLGVE+DDGIP NSIWN ASFGEST
Sbjct: 61 AILDQKVVEDLARNPAVASIHENKGRKLHTTSSWKFLGVEHDDGIPHNSIWNRASFGEST 120
Query: 133 IIANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKFHCNRKLIGARYFNKGYEAIVGS 192
II NLDTGVWPESKSF+DEGYGP+PT+WKGSCEGGSKFHCNRKLIGARYFNKGY A GS
Sbjct: 121 IIGNLDTGVWPESKSFNDEGYGPVPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAYAGS 180
Query: 193 LNASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKARVVAYKVCWPQQS 252
LNASYETARD++GHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKA V AYKVCWPQ
Sbjct: 181 LNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKALVAAYKVCWPQVD 240
Query: 253 SGGGCFDADILAAIEAAISDGVDVLSLSLGGNSKEFLDDVTAIGAFHAFQNGIVVVCSAG 312
SGGGCFDADILAAIEAAISDGVD+LSLSLGG +K+F +DVTAIGAFHA Q GI+VVCSAG
Sbjct: 241 SGGGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSEDVTAIGAFHAVQQGIIVVCSAG 300
Query: 313 NSGPALGTVENVAPWLVTVAASTINREFTNYLALGNQKHVKGASLSDKILPTQKFYPLIN 372
NSGPA GT+EN APW++TV ASTINR+FT+Y+ALGN+KH+ K+L KF L+
Sbjct: 301 NSGPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKHI-------KVLFLIKFI-LMG 360
Query: 373 AADAKANNVSADDARLCQDGMLDPEEVKGKIIVCLRGENGRVDKGYAAAQAGAVGMILAN 432
++ + + ++LCQ G LDP++VKGKII+CLRGEN RVDKGYAAAQAGAVGMILAN
Sbjct: 361 SSYSIEKTLMNPCSQLCQAGSLDPKKVKGKIILCLRGENARVDKGYAAAQAGAVGMILAN 420
Query: 433 TEQNGDELMADAHLLPASHVSYTDGESIYQYINSTKTPMAYMTHVRTELGVKPAPFMAAF 492
EQNGDEL+ADAHLLP SHVSYTDG+SIYQYIN TKTPMAYMTHVRTELG+KPAP MA+F
Sbjct: 421 AEQNGDELIADAHLLPVSHVSYTDGQSIYQYINFTKTPMAYMTHVRTELGIKPAPVMASF 480
Query: 493 SSRGPNTIEESILKPDITAPGVNIMAAYSEDTSPSGSPFDNRRIPFNIISGTSMSCPHIS 552
SSRGPNTIEESILKPDITAPGVNI+AAYSED SPSGS FDNRRIPFNI+SGTSMSCPHIS
Sbjct: 481 SSRGPNTIEESILKPDITAPGVNILAAYSEDASPSGSLFDNRRIPFNIVSGTSMSCPHIS 540
Query: 553 GIVGLLKTLYPQWSPAAIKSAIMTTAETRANDLHPILDTTKVKANPFAYGAGHVRPNRAV 612
GIVGLLKTLYP WSPAAIKSAIMTTAETRANDLHPIL+T +KANPFAYGAGHV+PNRA+
Sbjct: 541 GIVGLLKTLYPNWSPAAIKSAIMTTAETRANDLHPILNTVNLKANPFAYGAGHVQPNRAM 600
Query: 613 NPGLVYDLTTKDYLNFLCARGYNETKITKFSNTSFVCSKSFKLTDFNYPSISIPNLKSGP 672
NPGLVYDLTT DY+NFLCA+GYN+++I+KFS TSFVCSKSFKLTDFNYPSISIP++KSG
Sbjct: 601 NPGLVYDLTTNDYMNFLCAQGYNKSQISKFSATSFVCSKSFKLTDFNYPSISIPDMKSGV 660
Query: 673 VTIKRTVKNVGSPSTYVARVKVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSKNQG 732
VTI R VKNVG PSTYVARVKVP GVSVSV+P TL+FTGIDEEK+F+V + SVAN+K++G
Sbjct: 661 VTINRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFTGIDEEKSFKVVIGSVANNKHRG 720
Query: 733 YVFGSLAWSDGKHYVRSPIVVNLG 757
YVFGSL W DGKH+VRSPIVVNLG
Sbjct: 721 YVFGSLIWEDGKHHVRSPIVVNLG 736
BLAST of Sed0018375 vs. NCBI nr
Match:
XP_023553316.1 (subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1209.5 bits (3128), Expect = 0.0e+00
Identity = 595/767 (77.57%), Postives = 671/767 (87.48%), Query Frame = 0
Query: 3 FQLKTFILLHLV------------QSYIVYLGSHSHGLHPSPASLRIASESHYSLLGSML 62
F L +FILL + +SYIVYLGSHSHG +PS LRIA+ESHYSLLGS+L
Sbjct: 4 FNLSSFILLFFLFPLFQTSTIATQKSYIVYLGSHSHGSNPSSVDLRIATESHYSLLGSLL 63
Query: 63 GSNEEAKEAIFYSYNRHINGFAAILDQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLG 122
GSNE AKEAIFYSYNRHINGFAA+LD VA DL +HP V+SV+ENK RKLHTTSSW FL
Sbjct: 64 GSNEIAKEAIFYSYNRHINGFAAVLDHKVARDLTRHPAVLSVHENKMRKLHTTSSWEFLE 123
Query: 123 VENDDGIPANSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKF 182
+EN +G P NSIWN A+FGEST+I NLDTGVWPESKSFSDEGYG IP++W+GSCEGGS F
Sbjct: 124 LENGEGTPPNSIWNGANFGESTVIGNLDTGVWPESKSFSDEGYGAIPSRWRGSCEGGSNF 183
Query: 183 HCNRKLIGARYFNKGYEAIVGSLNASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNG 242
HCN+KLIGARYFNKGY A+ GSL+ S++TARD DGHGTHTLSTAGGNF+SGANVFGNGNG
Sbjct: 184 HCNKKLIGARYFNKGYTALAGSLDGSFDTARDHDGHGTHTLSTAGGNFVSGANVFGNGNG 243
Query: 243 TAKGGSPKARVVAYKVCWPQQSSGGGCFDADILAAIEAAISDGVDVLSLSLGGNSKEFLD 302
TAKGGSPKA V AYKVCWP GG C DADILAAIEAAI+DGVDVLSLSLG S EF D
Sbjct: 244 TAKGGSPKAFVAAYKVCWP-TFHGGQCSDADILAAIEAAITDGVDVLSLSLGRGSTEFFD 303
Query: 303 DVTAIGAFHAFQNGIVVVCSAGNSGPALGTVENVAPWLVTVAASTINREFTNYLALGNQK 362
DVTAIG+FHA Q GIVVVCS GNSGP +VENVAPWL TVAASTI R+FT+Y+ALGN+K
Sbjct: 304 DVTAIGSFHAVQQGIVVVCSGGNSGPDPQSVENVAPWLFTVAASTITRQFTSYVALGNKK 363
Query: 363 HVKGASLSDKILPTQKFYPLINAADAKANNVSADDARLCQDGMLDPEEVKGKIIVCLR-G 422
H+ GAS+SDKILP Q+FYPLI + DAKA N+S + A+LC +G LDP +VKGKIIVC+R G
Sbjct: 364 HITGASISDKILPAQQFYPLITSEDAKAINISVETAKLCVEGSLDPRKVKGKIIVCVRGG 423
Query: 423 ENGRVDKGYAAAQAGAVGMILANTEQNGDELMADAHLLPASHVSYTDGESIYQYINSTKT 482
++ RVDKGY AAQAGAVGMILAN E++G+EL+ADAHLLP SH+SY DGE++Y+YINSTKT
Sbjct: 424 DSARVDKGYVAAQAGAVGMILANNEEDGNELIADAHLLPVSHISYIDGETVYEYINSTKT 483
Query: 483 PMAYMTHVRTELGVKPAPFMAAFSSRGPNTIEESILKPDITAPGVNIMAAYSEDTSPSGS 542
P+AYMTHVRTE G+KPAP MA+FSSRGPN+IEESILKPDITAPGVNI+AAYSED SPSGS
Sbjct: 484 PVAYMTHVRTETGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYSEDASPSGS 543
Query: 543 PFDNRRIPFNIISGTSMSCPHISGIVGLLKTLYPQWSPAAIKSAIMTTAETRANDLHPIL 602
PFDNRRIPFN++SGTSMSCPHISGIVGLLKT+YP+WSPAAIKSAIMTTAETRANDLHPIL
Sbjct: 544 PFDNRRIPFNVVSGTSMSCPHISGIVGLLKTIYPKWSPAAIKSAIMTTAETRANDLHPIL 603
Query: 603 DTTKVKANPFAYGAGHVRPNRAVNPGLVYDLTTKDYLNFLCARGYNETKITKFSNTSFVC 662
D+TK+ ANP AYGAGHV PNRA NPGLVYDLTT DYLNFLCARGYN+ +++KFSNTSFVC
Sbjct: 604 DSTKLVANPLAYGAGHVHPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVC 663
Query: 663 SKSFKLTDFNYPSISIPNLKSGPVTIKRTVKNVGSPSTYVARVKVPPGVSVSVKPSTLEF 722
KSFKLTDFNYPSISI N+KSGPVTIKRTVKNVGSPSTYV RVKVPPGV VSV+PSTL+F
Sbjct: 664 LKSFKLTDFNYPSISISNMKSGPVTIKRTVKNVGSPSTYVVRVKVPPGVLVSVEPSTLKF 723
Query: 723 TGIDEEKTFQVELQSVANSKNQGYVFGSLAWSDGKHYVRSPIVVNLG 757
+ DEEKTF+V +S+AN+K++GYVFGSL W DGKH+VRS IVVNLG
Sbjct: 724 SRTDEEKTFKVVFRSLANNKHRGYVFGSLKWLDGKHHVRSSIVVNLG 769
BLAST of Sed0018375 vs. NCBI nr
Match:
XP_022932414.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])
HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 591/743 (79.54%), Postives = 664/743 (89.37%), Query Frame = 0
Query: 15 QSYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHINGFAAI 74
+SYIVYLGSHSHG +PS LRIA+ESHYSLLGS+LGSNE AKEAIFYSY+RHINGFAA+
Sbjct: 28 KSYIVYLGSHSHGSNPSSVDLRIATESHYSLLGSLLGSNEIAKEAIFYSYHRHINGFAAV 87
Query: 75 LDQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLGVENDDGIPANSIWNLASFGESTII 134
LD VA+DL +HP VVSV+ENK RKLHTTSSW FL +EN +G P NSIWN A+FGESTII
Sbjct: 88 LDHKVAQDLTRHPAVVSVHENKMRKLHTTSSWEFLDLENGEGTPPNSIWNGANFGESTII 147
Query: 135 ANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKFHCNRKLIGARYFNKGYEAIVGSLN 194
ANLDTGVWPESKSF+DEGYG IP++W+GSCEGGS FHCNRKLIGARYFNKGY A+ GSL+
Sbjct: 148 ANLDTGVWPESKSFNDEGYGAIPSRWRGSCEGGSNFHCNRKLIGARYFNKGYTALAGSLD 207
Query: 195 ASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKARVVAYKVCWPQQSSG 254
S++TARD DGHGTHTLSTAGGNF+SGANVFGNGNGTAKGGSPKA V AYKVCWP G
Sbjct: 208 DSFDTARDHDGHGTHTLSTAGGNFVSGANVFGNGNGTAKGGSPKAFVAAYKVCWP-TFHG 267
Query: 255 GGCFDADILAAIEAAISDGVDVLSLSLGGNSKEFLDDVTAIGAFHAFQNGIVVVCSAGNS 314
G C DADILAAIEAAI+DGVDVLSLSLG S EF DDVTAIG+FHA Q GIVVVCS GNS
Sbjct: 268 GQCSDADILAAIEAAITDGVDVLSLSLGRGSTEFFDDVTAIGSFHAVQQGIVVVCSGGNS 327
Query: 315 GPALGTVENVAPWLVTVAASTINREFTNYLALGNQKHVKGASLSDKILPTQKFYPLINAA 374
GP +VENVAPWL TVAASTI R+FT+Y+ALGN+KH+ GAS+SDKILP Q+FYPLI +
Sbjct: 328 GPDPQSVENVAPWLFTVAASTITRQFTSYVALGNKKHITGASISDKILPAQQFYPLITSV 387
Query: 375 DAKANNVSADDARLCQDGMLDPEEVKGKIIVCLR-GENGRVDKGYAAAQAGAVGMILANT 434
DAKA N+S + A+LC +G LDP +VKGKIIVC++ G++ RVDKGY AAQAGAVGMILAN+
Sbjct: 388 DAKAINISVETAKLCVEGSLDPRKVKGKIIVCVKGGDSARVDKGYVAAQAGAVGMILANS 447
Query: 435 EQNGDELMADAHLLPASHVSYTDGESIYQYINSTKTPMAYMTHVRTELGVKPAPFMAAFS 494
E++G+EL+ADAHLLP SH+SY DGE +Y+YINSTKTP+AYMT VRTE G+KPAP MA+FS
Sbjct: 448 EEDGNELIADAHLLPVSHISYIDGEIVYEYINSTKTPVAYMTQVRTETGIKPAPVMASFS 507
Query: 495 SRGPNTIEESILKPDITAPGVNIMAAYSEDTSPSGSPFDNRRIPFNIISGTSMSCPHISG 554
SRGPN+IEESILKPDITAPGVNI+AAYSED SPSGSPFDNRRIPFN++SGTSMSCPHISG
Sbjct: 508 SRGPNSIEESILKPDITAPGVNIIAAYSEDASPSGSPFDNRRIPFNVVSGTSMSCPHISG 567
Query: 555 IVGLLKTLYPQWSPAAIKSAIMTTAETRANDLHPILDTTKVKANPFAYGAGHVRPNRAVN 614
IV LLKT+YP+WSPAAIKSAIMTTAETRANDLHPILD+TK+ ANP AYGAGHV PNRA N
Sbjct: 568 IVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDSTKLVANPLAYGAGHVHPNRAAN 627
Query: 615 PGLVYDLTTKDYLNFLCARGYNETKITKFSNTSFVCSKSFKLTDFNYPSISIPNLKSGPV 674
PGLVYDLTT DYLNFLCARGYN+T+++KFSNTSFVCSKSFKLTDFNYPSISIP +KS PV
Sbjct: 628 PGLVYDLTTNDYLNFLCARGYNKTQLSKFSNTSFVCSKSFKLTDFNYPSISIPKMKSEPV 687
Query: 675 TIKRTVKNVGSPSTYVARVKVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSKNQGY 734
TIKRTVKNVGSPSTYVARVKVPPGV VSV+PSTL+FT DEEKTF+V +SV N+K++GY
Sbjct: 688 TIKRTVKNVGSPSTYVARVKVPPGVLVSVEPSTLKFTRTDEEKTFKVVFRSVTNTKHRGY 747
Query: 735 VFGSLAWSDGKHYVRSPIVVNLG 757
VFGSL W DGKH+VRS IVVNLG
Sbjct: 748 VFGSLKWLDGKHHVRSSIVVNLG 769
BLAST of Sed0018375 vs. ExPASy Swiss-Prot
Match:
F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)
HSP 1 Score: 905.6 bits (2339), Expect = 3.8e-262
Identity = 460/745 (61.74%), Postives = 558/745 (74.90%), Query Frame = 0
Query: 13 LVQSYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHINGFA 72
L +SYIVYLGSH+H S A L + SH + L S +GS+E AKEAIFYSY RHINGFA
Sbjct: 38 LKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFA 97
Query: 73 AILDQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLGVENDDGIPANSIWNLASFGEST 132
AILD+N A ++AKHP VVSV NKGRKLHTT SW F+ + + + +S+WN A +GE T
Sbjct: 98 AILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDT 157
Query: 133 IIANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKFHCNRKLIGARYFNKGYEAIVG- 192
IIANLDTGVWPESKSFSDEGYG +P +WKG C CNRKLIGARYFNKGY A G
Sbjct: 158 IIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTGL 217
Query: 193 SLNASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKARVVAYKVCWPQQ 252
NASYET RD DGHG+HTLSTA GNF+ GANVFG GNGTA GGSPKARV AYKVCWP
Sbjct: 218 PSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWP-P 277
Query: 253 SSGGGCFDADILAAIEAAISDGVDVLSLSLGGNSKEFLDDVTAIGAFHAFQNGIVVVCSA 312
G CFDADILAAIEAAI DGVDVLS S+GG++ +++ D AIG+FHA +NG+ VVCSA
Sbjct: 278 VDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSA 337
Query: 313 GNSGPALGTVENVAPWLVTVAASTINREFTNYLALGNQKHVKGASLSDKILPTQKFYPLI 372
GNSGP GTV NVAPW++TV AS+++REF ++ L N + KG SLS K LP +K Y LI
Sbjct: 338 GNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLS-KPLPEEKMYSLI 397
Query: 373 NAADAKANNVSADDARLCQDGMLDPEEVKGKIIVCLRGENGRVDKGYAAAQAGAVGMILA 432
+AADA N + DA LC+ G LDP++VKGKI+VCLRG+N RVDKG AA AGA GM+L
Sbjct: 398 SAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLC 457
Query: 433 NTEQNGDELMADAHLLPASHVSYTDGESIYQYINSTKTPMAYMTHVRTELGVKPAPFMAA 492
N + +G+E+++DAH+LPAS + Y DGE+++ Y++STK P Y+ L KPAPFMA+
Sbjct: 458 NDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMAS 517
Query: 493 FSSRGPNTIEESILKPDITAPGVNIMAAYSEDTSPSGSPFDNRRIPFNIISGTSMSCPHI 552
FSSRGPNTI ILKPDITAPGVNI+AA++E T P+ DNRR PFN SGTSMSCPHI
Sbjct: 518 FSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHI 577
Query: 553 SGIVGLLKTLYPQWSPAAIKSAIMTTAETRANDLHPILDTTKVKANPFAYGAGHVRPNRA 612
SG+VGLLKTL+P WSPAAI+SAIMTT+ TR N P++D + KANPF+YG+GHV+PN+A
Sbjct: 578 SGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKA 637
Query: 613 VNPGLVYDLTTKDYLNFLCARGYNETKITKFS-NTSFVCSKSFKLTDFNYPSISIPNLKS 672
+PGLVYDLTT DYL+FLCA GYN T + F+ + + C + L DFNYPSI++PNL +
Sbjct: 638 AHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNL-T 697
Query: 673 GPVTIKRTVKNVGSPSTYVARVKVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSKN 732
G +T+ R +KNVG P+TY AR + P GV VSV+P L F E K FQ+ L+ + + +
Sbjct: 698 GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVTPS 757
Query: 733 QGYVFGSLAWSDGKHYVRSPIVVNL 756
GYVFG L W+D HYVRSPIVV L
Sbjct: 758 -GYVFGELTWTDSHHYVRSPIVVQL 776
BLAST of Sed0018375 vs. ExPASy Swiss-Prot
Match:
Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)
HSP 1 Score: 899.0 bits (2322), Expect = 3.5e-260
Identity = 446/743 (60.03%), Postives = 554/743 (74.56%), Query Frame = 0
Query: 16 SYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHINGFAAIL 75
SY+VY G+HSH + ++ E+HY LGS GS E A +AIFYSY +HINGFAA L
Sbjct: 31 SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90
Query: 76 DQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLGVENDDGIPANSIWNLASFGESTIIA 135
D ++A +++KHP VVSV NK KLHTT SW FLG+E++ +P++SIW A FGE TIIA
Sbjct: 91 DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150
Query: 136 NLDTGVWPESKSFSDEGYGPIPTKWKGSCEG--GSKFHCNRKLIGARYFNKGYEAIVGSL 195
NLDTGVWPESKSF DEG GPIP++WKG C+ + FHCNRKLIGARYFNKGY A VG L
Sbjct: 151 NLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHL 210
Query: 196 NASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKARVVAYKVCWPQQSS 255
N+S+++ RD DGHG+HTLSTA G+F+ G ++FG GNGTAKGGSP+ARV AYKVCWP
Sbjct: 211 NSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWP-PVK 270
Query: 256 GGGCFDADILAAIEAAISDGVDVLSLSLGGNSKEFLDDVTAIGAFHAFQNGIVVVCSAGN 315
G C+DAD+LAA +AAI DG DV+S+SLGG F +D AIG+FHA + IVVVCSAGN
Sbjct: 271 GNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGN 330
Query: 316 SGPALGTVENVAPWLVTVAASTINREFTNYLALGNQKHVKGASLSDKILPTQKFYPLINA 375
SGPA TV NVAPW +TV AST++REF + L LGN KH KG SLS LP KFYP++ +
Sbjct: 331 SGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMAS 390
Query: 376 ADAKANNVSADDARLCQDGMLDPEEVKGKIIVCLRGENGRVDKGYAAAQAGAVGMILANT 435
+AKA N SA DA+LC+ G LDP + KGKI+VCLRG+NGRV+KG A A G +GM+L NT
Sbjct: 391 VNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENT 450
Query: 436 EQNGDELMADAHLLPASHVSYTDGESIYQYINSTKTPMAYMTHVRTELGVKPAPFMAAFS 495
G++L+AD H+LPA+ ++ D ++ +YI+ TK P+A++T RT+LG+KPAP MA+FS
Sbjct: 451 YVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFS 510
Query: 496 SRGPNTIEESILKPDITAPGVNIMAAYSEDTSPSGSPFDNRRIPFNIISGTSMSCPHISG 555
S+GP+ + ILKPDITAPGV+++AAY+ SP+ FD RR+ FN ISGTSMSCPHISG
Sbjct: 511 SKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISG 570
Query: 556 IVGLLKTLYPQWSPAAIKSAIMTTAETRANDLHPILDTTKVKANPFAYGAGHVRPNRAVN 615
I GLLKT YP WSPAAI+SAIMTTA + PI + T +KA PF++GAGHV+PN AVN
Sbjct: 571 IAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVN 630
Query: 616 PGLVYDLTTKDYLNFLCARGYNETKITKFSNTSFVCSK-SFKLTDFNYPSISIPNLKSGP 675
PGLVYDL KDYLNFLC+ GYN ++I+ FS +F CS L + NYPSI++PNL S
Sbjct: 631 PGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSK 690
Query: 676 VTIKRTVKNVGSPSTYVARVKVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSKNQG 735
VT+ RTVKNVG PS Y +V P GV V+VKP++L FT + E+KTF+V L + +G
Sbjct: 691 VTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKG 750
Query: 736 YVFGSLAWSDGKHYVRSPIVVNL 756
YVFG L WSD KH VRSPIVV L
Sbjct: 751 YVFGELVWSDKKHRVRSPIVVKL 772
BLAST of Sed0018375 vs. ExPASy Swiss-Prot
Match:
I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)
HSP 1 Score: 760.8 bits (1963), Expect = 1.5e-218
Identity = 422/779 (54.17%), Postives = 525/779 (67.39%), Query Frame = 0
Query: 2 SFQLKTFILLHLVQS---YIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKE 61
SF L TF+L + S YIVY+G+HSHG P+ A L +A++SHY LLGS+ GS E+AKE
Sbjct: 14 SFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKE 73
Query: 62 AIFYSYNRHINGFAAILDQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLGVENDDGIP 121
AI YSYNRHINGFAA+L++ A D+AK+P VVSV +K KLHTT SW FLG+
Sbjct: 74 AIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG--- 133
Query: 122 ANSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPTKWKGS-CE-----GGSKFHC 181
NS W FGE+TII N+DTGVWPES+SFSD+GYG +P+KW+G C+ G K C
Sbjct: 134 QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMKNTC 193
Query: 182 NRKLIGARYFNKGYEAIVGSLNASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNGTA 241
NRKLIGARY+NK +EA G L+ TARD GHGTHTLSTAGGNF+ GA VF GNGTA
Sbjct: 194 NRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTA 253
Query: 242 KGGSPKARVVAYKVCWPQQSSGGGCFDADILAAIEAAISDGVDVLSLSLGGN----SKEF 301
KGGSP+ARV AYKVCW + C+ AD+LAAI+ AI DGVDV+++S G + ++
Sbjct: 254 KGGSPRARVAAYKVCW-SLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGI 313
Query: 302 LDDVTAIGAFHAFQNGIVVVCSAGNSGPALGTVENVAPWLVTVAASTINREFTNYLALGN 361
D +IGAFHA I++V SAGN GP GTV NVAPW+ T+AAST++R+F++ L + N
Sbjct: 314 FTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTINN 373
Query: 362 QKHVKGASLSDKILPTQKFYPLINAADAKANNVSADDARLCQDGMLDPEEVKGKIIVCLR 421
Q ++GASL + P Q F LI + DAK N + DA+LC+ G LD +V GKI++C R
Sbjct: 374 QL-IEGASLFVNLPPNQAF-SLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTR 433
Query: 422 -GENGRVDKGYAAAQAGAVGMILANTEQNGDELMADAHLLPASHVSYTDGESIYQYINST 481
G+ V +G A AGA GMIL N QNG L A+ H+ + +S + +T
Sbjct: 434 EGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTT 493
Query: 482 ---------KT-PMAYMTHVRTELGVKPAPFMAAFSSRGPNTIEESILKPDITAPGVNIM 541
KT M+ RT G KPAP MA+FSSRGPN I+ SILKPD+TAPGVNI+
Sbjct: 494 AIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNIL 553
Query: 542 AAYSEDTSPSGSPFDNRR-IPFNIISGTSMSCPHISGIVGLLKTLYPQWSPAAIKSAIMT 601
AAYSE S S DNRR FN++ GTSMSCPH SGI GLLKT +P WSPAAIKSAIMT
Sbjct: 554 AAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMT 613
Query: 602 TAETRANDLHPILDT-TKVKANPFAYGAGHVRPNRAVNPGLVYDLTTKDYLNFLCARGYN 661
TA T N PI D K A+ FAYG+GHVRP+ A+ PGLVYDL+ DYLNFLCA GY+
Sbjct: 614 TATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYD 673
Query: 662 ETKITKFS-NTSFVCSKSFKLTDFNYPSISIPNLKSGPVTIKRTVKNVGSPSTYVARVKV 721
+ I+ + N +F+CS S + D NYPSI++PNL+ PVTI RTV NVG PSTY +
Sbjct: 674 QQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTYTVSTRS 733
Query: 722 PPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSKNQGYVFGSLAWSDGKHYVRSPIVV 754
P G S++V P +L FT I E KTF+V +Q+ + + + Y FG L W+DGKH VRSPI V
Sbjct: 734 PNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSPITV 786
BLAST of Sed0018375 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 646.4 bits (1666), Expect = 4.1e-184
Identity = 347/736 (47.15%), Postives = 470/736 (63.86%), Query Frame = 0
Query: 28 LHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHINGFAAILDQNVAEDLAKHP 87
+H + + + + + H + S L S ++ E + Y+Y I+GF+ L Q A+ L P
Sbjct: 34 VHMAKSQMPSSFDLHSNWYDSSLRSISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQP 93
Query: 88 GVVSVNENKGRKLHTTSSWRFLGVENDDGIPANSIWNLASFGESTIIANLDTGVWPESKS 147
GV+SV +LHTT + FLG++ ++ A ++ LDTGVWPESKS
Sbjct: 94 GVISVLPEHRYELHTTRTPLFLGLDEHTA----DLFPEAGSYSDVVVGVLDTGVWPESKS 153
Query: 148 FSDEGYGPIPTKWKGSCEGGSKFH---CNRKLIGARYFNKGYEAIVGSLNASYE--TARD 207
+SDEG+GPIP+ WKG CE G+ F CNRKLIGAR+F +GYE+ +G ++ S E + RD
Sbjct: 154 YSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRD 213
Query: 208 DDGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKARVVAYKVCWPQQSSGGGCFDADI 267
DDGHGTHT STA G+ + GA++ G +GTA+G +P+ARV YKVCW GGCF +DI
Sbjct: 214 DDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL-----GGCFSSDI 273
Query: 268 LAAIEAAISDGVDVLSLSLGGNSKEFLDDVTAIGAFHAFQNGIVVVCSAGNSGPALGTVE 327
LAAI+ AI+D V+VLS+SLGG ++ D AIGAF A + GI+V CSAGN+GP+ ++
Sbjct: 274 LAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLS 333
Query: 328 NVAPWLVTVAASTINREFTNYLALGNQKHVKGASLSDKILPTQKFYPLINAADAKANNVS 387
NVAPW+ TV A T++R+F LGN K+ G SL K P I A +A +
Sbjct: 334 NVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNAS----N 393
Query: 388 ADDARLCQDGMLDPEEVKGKIIVCLRGENGRVDKGYAAAQAGAVGMILANTEQNGDELMA 447
A + LC G L PE+VKGKI++C RG N RV KG AG VGMILANT NG+EL+A
Sbjct: 394 ATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVA 453
Query: 448 DAHLLPASHVSYTDGESIYQYINSTKTPMAYMTHVRTELGVKPAPFMAAFSSRGPNTIEE 507
DAHLLPA+ V G+ I Y+ + P A ++ + T +GVKP+P +AAFSSRGPN+I
Sbjct: 454 DAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITP 513
Query: 508 SILKPDITAPGVNIMAAYSEDTSPSGSPFDNRRIPFNIISGTSMSCPHISGIVGLLKTLY 567
+ILKPD+ APGVNI+AA++ P+G D+RR+ FNIISGTSMSCPH+SG+ LLK+++
Sbjct: 514 NILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVH 573
Query: 568 PQWSPAAIKSAIMTTAETRANDLHPILD-TTKVKANPFAYGAGHVRPNRAVNPGLVYDLT 627
P+WSPAAI+SA+MTTA D P+LD T + PF +GAGHV P A NPGL+YDLT
Sbjct: 574 PEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLT 633
Query: 628 TKDYLNFLCARGYNETKITKFSNTSFVC--SKSFKLTDFNYPSISIPNLKSGPVTIKRTV 687
T+DYL FLCA Y +I S ++ C SKS+ + D NYPS ++ G RTV
Sbjct: 634 TEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTV 693
Query: 688 KNVGSPSTYVARV-KVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSKNQGYVFGSL 747
+VG TY +V GV +SV+P+ L F +E+K++ V ++ + FGS+
Sbjct: 694 TSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSI 753
Query: 748 AWSDGKHYVRSPIVVN 755
WSDGKH V SP+ ++
Sbjct: 754 EWSDGKHVVGSPVAIS 755
BLAST of Sed0018375 vs. ExPASy Swiss-Prot
Match:
Q9FK76 (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 SV=1)
HSP 1 Score: 590.5 bits (1521), Expect = 2.7e-167
Identity = 340/779 (43.65%), Postives = 463/779 (59.44%), Query Frame = 0
Query: 15 QSYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHINGFAAI 74
Q YIVY G H + E H+S L S+ S E+A+ ++ YSY INGFAA
Sbjct: 25 QVYIVYFGEHK-----GDKAFHEIEEHHHSYLQSVKESEEDARASLLYSYKHSINGFAAE 84
Query: 75 LDQNVAEDLAKHPGVVSVNENKGRK--LHTTSSWRFLGV---ENDDGIP----------- 134
L + A L K VVSV ++ RK HTT SW F+G+ E D +P
Sbjct: 85 LTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDVPRRKNDADDRFR 144
Query: 135 -ANSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKF---HCNR 194
+ A G+ I+ LD+GVWPESKSF+D+G GP+P WKG C+ G F HCNR
Sbjct: 145 VGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGICQTGVAFNSSHCNR 204
Query: 195 KLIGARYFNKGYEAIVGSLNAS----YETARDDDGHGTHTLSTAGGNFISGANVFGN-GN 254
K+IGARY+ KGYE G+ NA+ + + RD DGHG+HT STA G + GA+ G
Sbjct: 205 KIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGRRVLGASALGGFAK 264
Query: 255 GTAKGGSPKARVVAYKVCWPQQSS----GGGCFDADILAAIEAAISDGVDVLSLSLGGNS 314
G+A GG+P AR+ YK CW + ++ G C + D+LAAI+ AI+DGV V+S+S+G
Sbjct: 265 GSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADGVHVISISIGTTE 324
Query: 315 K-EFLDDVTAIGAFHAFQNGIVVVCSAGNSGPALGTVENVAPWLVTVAASTINREFTNYL 374
F D A+GA HA + IVV SAGNSGP GT+ N+APW++TV AST++R F L
Sbjct: 325 PFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVGASTLDRAFVGGL 384
Query: 375 ALGNQKHVKGASLSDKILPTQKFYPLINAADAKANNVSADDARLCQDGMLDPEEVKGKII 434
LGN +K S++ KF PL+ A++ ++ ++ C L PE V GK++
Sbjct: 385 VLGNGYTIKTDSIT--AFKMDKFAPLVYASNVVVPGIALNETSQCLPNSLKPELVSGKVV 444
Query: 435 VCLRGENGRVDKGYAAAQAGAVGMILANTEQNGDELMADAHLLPASHVSYTDGESIYQYI 494
+CLRG R+ KG +AG GMIL N NG+E+ +D+H +P + V+ T + I +YI
Sbjct: 445 LCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPTAGVTPTVVDKILEYI 504
Query: 495 NSTKTPMAYMTHVRTELGVKPAPFMAAFSSRGPNTIEESILKPDITAPGVNIMAAYSEDT 554
+ K P A++ +T + AP M FSSRGPN ++ +ILKPDITAPG+ I+AA+S
Sbjct: 505 KTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDITAPGLYILAAWSGAD 564
Query: 555 SPSGSPFDNRRIPFNIISGTSMSCPHISGIVGLLKTLYPQWSPAAIKSAIMTTAETRAND 614
SPS D R +NI SGTSMSCPH++G + LLK ++P+WS AAI+SA+MTTA +
Sbjct: 565 SPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSSAAIRSALMTTAWMTNDK 624
Query: 615 LHPILDTTKVKANPFAYGAGHVRPNRAVNPGLVYDLTTKDYLNFLCARGYNETKITKFSN 674
PI DTT + ANPFA G+GH RP +A +PGLVYD + + YL + C+ IT
Sbjct: 625 KKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYLLYGCS-----VNITNIDP 684
Query: 675 TSFVCSKSFKLTDFNYPSISIPNLKSGPVTIKRTVKNVG---SPSTYVARVKVPPGVSVS 734
T SK + NYPSI++PNLK VT+KRTV NVG S STY+ VK P G+SV
Sbjct: 685 TFKCPSKIPPGYNHNYPSIAVPNLKK-TVTVKRTVTNVGTGNSTSTYLFSVKPPSGISVK 744
Query: 735 VKPSTLEFTGIDEEKTFQVELQ----SVANSKNQG-YVFGSLAWSDGKHYVRSPIVVNL 756
P+ L F I +++ F++ ++ V N+ +G Y FG +W+D H VRSPI V+L
Sbjct: 745 AIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWFSWTDKVHVVRSPIAVSL 790
BLAST of Sed0018375 vs. ExPASy TrEMBL
Match:
A0A1S4DX85 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491136 PE=3 SV=1)
HSP 1 Score: 1297.7 bits (3357), Expect = 0.0e+00
Identity = 632/747 (84.61%), Postives = 694/747 (92.90%), Query Frame = 0
Query: 10 LLHLVQSYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHIN 69
+ +L+QSYIVYLGSHSHG +PS A L IA+ESHYSLLGS+LGSNE AKEAIFYSYNRHIN
Sbjct: 1 IANLLQSYIVYLGSHSHGSNPSAADLEIATESHYSLLGSLLGSNEAAKEAIFYSYNRHIN 60
Query: 70 GFAAILDQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLGVENDDGIPANSIWNLASFG 129
GFAAILDQ V EDLA++P V S++ENKGRKLHTTSSW+FLGVE+DDGIP NSIWN ASFG
Sbjct: 61 GFAAILDQKVVEDLARNPAVASIHENKGRKLHTTSSWKFLGVEHDDGIPHNSIWNRASFG 120
Query: 130 ESTIIANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKFHCNRKLIGARYFNKGYEAI 189
ESTII NLDTGVWPESKSF+DEGYGP+PT+WKGSCEGGSKFHCNRKLIGARYFNKGY A
Sbjct: 121 ESTIIGNLDTGVWPESKSFNDEGYGPVPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAY 180
Query: 190 VGSLNASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKARVVAYKVCWP 249
GSLNASYETARD++GHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKA V AYKVCWP
Sbjct: 181 AGSLNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKALVAAYKVCWP 240
Query: 250 QQSSGGGCFDADILAAIEAAISDGVDVLSLSLGGNSKEFLDDVTAIGAFHAFQNGIVVVC 309
Q SGGGCFDADILAAIEAAISDGVD+LSLSLGG +K+F +DVTAIGAFHA Q GI+VVC
Sbjct: 241 QVDSGGGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSEDVTAIGAFHAVQQGIIVVC 300
Query: 310 SAGNSGPALGTVENVAPWLVTVAASTINREFTNYLALGNQKHVKGASLSDKILPTQKFYP 369
SAGNSGPA GT+EN APW++TV ASTINR+FT+Y+ALGN+KH+KGASLSDKILP QKFYP
Sbjct: 301 SAGNSGPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKHIKGASLSDKILPEQKFYP 360
Query: 370 LINAADAKANNVSADDARLCQDGMLDPEEVKGKIIVCLRGENGRVDKGYAAAQAGAVGMI 429
LINAADAKANNVS+D A+LCQ G LDP++VKGKII+CLRGEN RVDKGYAAAQAGAVGMI
Sbjct: 361 LINAADAKANNVSSDVAQLCQAGSLDPKKVKGKIILCLRGENARVDKGYAAAQAGAVGMI 420
Query: 430 LANTEQNGDELMADAHLLPASHVSYTDGESIYQYINSTKTPMAYMTHVRTELGVKPAPFM 489
LAN EQNGDEL+ADAHLLP SHVSYTDG+SIYQYIN TKTPMAYMTHVRTELG+KPAP M
Sbjct: 421 LANAEQNGDELIADAHLLPVSHVSYTDGQSIYQYINFTKTPMAYMTHVRTELGIKPAPVM 480
Query: 490 AAFSSRGPNTIEESILKPDITAPGVNIMAAYSEDTSPSGSPFDNRRIPFNIISGTSMSCP 549
A+FSSRGPNTIEESILKPDITAPGVNI+AAYSED SPSGS FDNRRIPFNI+SGTSMSCP
Sbjct: 481 ASFSSRGPNTIEESILKPDITAPGVNILAAYSEDASPSGSLFDNRRIPFNIVSGTSMSCP 540
Query: 550 HISGIVGLLKTLYPQWSPAAIKSAIMTTAETRANDLHPILDTTKVKANPFAYGAGHVRPN 609
HISGIVGLLKTLYP WSPAAIKSAIMTTAETRANDLHPIL+T +KANPFAYGAGHV+PN
Sbjct: 541 HISGIVGLLKTLYPTWSPAAIKSAIMTTAETRANDLHPILNTVNLKANPFAYGAGHVQPN 600
Query: 610 RAVNPGLVYDLTTKDYLNFLCARGYNETKITKFSNTSFVCSKSFKLTDFNYPSISIPNLK 669
RA+NPGLVYDLTT DY+NFLCA+GYN+++I+KFS TSFVCSKSFKLTDFNYPSISIP++K
Sbjct: 601 RAMNPGLVYDLTTNDYMNFLCAQGYNKSQISKFSATSFVCSKSFKLTDFNYPSISIPDMK 660
Query: 670 SGPVTIKRTVKNVGSPSTYVARVKVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSK 729
SG VTI R VKNVG PSTYVARVKVP GVSVSV+P TL+FTGIDEEK+F+V + SVAN+K
Sbjct: 661 SGVVTINRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFTGIDEEKSFKVVIGSVANNK 720
Query: 730 NQGYVFGSLAWSDGKHYVRSPIVVNLG 757
++GYVFGSL W DGKH+VRSPIVVNLG
Sbjct: 721 HRGYVFGSLIWEDGKHHVRSPIVVNLG 747
BLAST of Sed0018375 vs. ExPASy TrEMBL
Match:
A0A5D3D7B7 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00140 PE=3 SV=1)
HSP 1 Score: 1229.2 bits (3179), Expect = 0.0e+00
Identity = 604/744 (81.18%), Postives = 671/744 (90.19%), Query Frame = 0
Query: 13 LVQSYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHINGFA 72
+ +SYIVYLGSHSHG +PS A L IA+ESHYSLLGS+LGSNE AKEAIFYSYNRHINGFA
Sbjct: 1 MAKSYIVYLGSHSHGSNPSAADLEIATESHYSLLGSLLGSNEAAKEAIFYSYNRHINGFA 60
Query: 73 AILDQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLGVENDDGIPANSIWNLASFGEST 132
AILDQ V EDLA++P V S++ENKGRKLHTTSSW+FLGVE+DDGIP NSIWN ASFGEST
Sbjct: 61 AILDQKVVEDLARNPAVASIHENKGRKLHTTSSWKFLGVEHDDGIPHNSIWNRASFGEST 120
Query: 133 IIANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKFHCNRKLIGARYFNKGYEAIVGS 192
II NLDTGVWPESKSF+DEGYGP+PT+WKGSCEGGSKFHCNRKLIGARYFNKGY A GS
Sbjct: 121 IIGNLDTGVWPESKSFNDEGYGPVPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAYAGS 180
Query: 193 LNASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKARVVAYKVCWPQQS 252
LNASYETARD++GHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKA V AYKVCWPQ
Sbjct: 181 LNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKALVAAYKVCWPQVD 240
Query: 253 SGGGCFDADILAAIEAAISDGVDVLSLSLGGNSKEFLDDVTAIGAFHAFQNGIVVVCSAG 312
SGGGCFDADILAAIEAAISDGVD+LSLSLGG +K+F +DVTAIGAFHA Q GI+VVCSAG
Sbjct: 241 SGGGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSEDVTAIGAFHAVQQGIIVVCSAG 300
Query: 313 NSGPALGTVENVAPWLVTVAASTINREFTNYLALGNQKHVKGASLSDKILPTQKFYPLIN 372
NSGPA GT+EN APW++TV ASTINR+FT+Y+ALGN+KH+ K+L KF L+
Sbjct: 301 NSGPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKHI-------KVLFLIKFI-LMG 360
Query: 373 AADAKANNVSADDARLCQDGMLDPEEVKGKIIVCLRGENGRVDKGYAAAQAGAVGMILAN 432
++ + + ++LCQ G LDP++VKGKII+CLRGEN RVDKGYAAAQAGAVGMILAN
Sbjct: 361 SSYSIEKTLMNPCSQLCQAGSLDPKKVKGKIILCLRGENARVDKGYAAAQAGAVGMILAN 420
Query: 433 TEQNGDELMADAHLLPASHVSYTDGESIYQYINSTKTPMAYMTHVRTELGVKPAPFMAAF 492
EQNGDEL+ADAHLLP SHVSYTDG+SIYQYIN TKTPMAYMTHVRTELG+KPAP MA+F
Sbjct: 421 AEQNGDELIADAHLLPVSHVSYTDGQSIYQYINFTKTPMAYMTHVRTELGIKPAPVMASF 480
Query: 493 SSRGPNTIEESILKPDITAPGVNIMAAYSEDTSPSGSPFDNRRIPFNIISGTSMSCPHIS 552
SSRGPNTIEESILKPDITAPGVNI+AAYSED SPSGS FDNRRIPFNI+SGTSMSCPHIS
Sbjct: 481 SSRGPNTIEESILKPDITAPGVNILAAYSEDASPSGSLFDNRRIPFNIVSGTSMSCPHIS 540
Query: 553 GIVGLLKTLYPQWSPAAIKSAIMTTAETRANDLHPILDTTKVKANPFAYGAGHVRPNRAV 612
GIVGLLKTLYP WSPAAIKSAIMTTAETRANDLHPIL+T +KANPFAYGAGHV+PNRA+
Sbjct: 541 GIVGLLKTLYPNWSPAAIKSAIMTTAETRANDLHPILNTVNLKANPFAYGAGHVQPNRAM 600
Query: 613 NPGLVYDLTTKDYLNFLCARGYNETKITKFSNTSFVCSKSFKLTDFNYPSISIPNLKSGP 672
NPGLVYDLTT DY+NFLCA+GYN+++I+KFS TSFVCSKSFKLTDFNYPSISIP++KSG
Sbjct: 601 NPGLVYDLTTNDYMNFLCAQGYNKSQISKFSATSFVCSKSFKLTDFNYPSISIPDMKSGV 660
Query: 673 VTIKRTVKNVGSPSTYVARVKVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSKNQG 732
VTI R VKNVG PSTYVARVKVP GVSVSV+P TL+FTGIDEEK+F+V + SVAN+K++G
Sbjct: 661 VTINRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFTGIDEEKSFKVVIGSVANNKHRG 720
Query: 733 YVFGSLAWSDGKHYVRSPIVVNLG 757
YVFGSL W DGKH+VRSPIVVNLG
Sbjct: 721 YVFGSLIWEDGKHHVRSPIVVNLG 736
BLAST of Sed0018375 vs. ExPASy TrEMBL
Match:
A0A6J1EWX5 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111438769 PE=3 SV=1)
HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 591/743 (79.54%), Postives = 664/743 (89.37%), Query Frame = 0
Query: 15 QSYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHINGFAAI 74
+SYIVYLGSHSHG +PS LRIA+ESHYSLLGS+LGSNE AKEAIFYSY+RHINGFAA+
Sbjct: 28 KSYIVYLGSHSHGSNPSSVDLRIATESHYSLLGSLLGSNEIAKEAIFYSYHRHINGFAAV 87
Query: 75 LDQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLGVENDDGIPANSIWNLASFGESTII 134
LD VA+DL +HP VVSV+ENK RKLHTTSSW FL +EN +G P NSIWN A+FGESTII
Sbjct: 88 LDHKVAQDLTRHPAVVSVHENKMRKLHTTSSWEFLDLENGEGTPPNSIWNGANFGESTII 147
Query: 135 ANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKFHCNRKLIGARYFNKGYEAIVGSLN 194
ANLDTGVWPESKSF+DEGYG IP++W+GSCEGGS FHCNRKLIGARYFNKGY A+ GSL+
Sbjct: 148 ANLDTGVWPESKSFNDEGYGAIPSRWRGSCEGGSNFHCNRKLIGARYFNKGYTALAGSLD 207
Query: 195 ASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKARVVAYKVCWPQQSSG 254
S++TARD DGHGTHTLSTAGGNF+SGANVFGNGNGTAKGGSPKA V AYKVCWP G
Sbjct: 208 DSFDTARDHDGHGTHTLSTAGGNFVSGANVFGNGNGTAKGGSPKAFVAAYKVCWP-TFHG 267
Query: 255 GGCFDADILAAIEAAISDGVDVLSLSLGGNSKEFLDDVTAIGAFHAFQNGIVVVCSAGNS 314
G C DADILAAIEAAI+DGVDVLSLSLG S EF DDVTAIG+FHA Q GIVVVCS GNS
Sbjct: 268 GQCSDADILAAIEAAITDGVDVLSLSLGRGSTEFFDDVTAIGSFHAVQQGIVVVCSGGNS 327
Query: 315 GPALGTVENVAPWLVTVAASTINREFTNYLALGNQKHVKGASLSDKILPTQKFYPLINAA 374
GP +VENVAPWL TVAASTI R+FT+Y+ALGN+KH+ GAS+SDKILP Q+FYPLI +
Sbjct: 328 GPDPQSVENVAPWLFTVAASTITRQFTSYVALGNKKHITGASISDKILPAQQFYPLITSV 387
Query: 375 DAKANNVSADDARLCQDGMLDPEEVKGKIIVCLR-GENGRVDKGYAAAQAGAVGMILANT 434
DAKA N+S + A+LC +G LDP +VKGKIIVC++ G++ RVDKGY AAQAGAVGMILAN+
Sbjct: 388 DAKAINISVETAKLCVEGSLDPRKVKGKIIVCVKGGDSARVDKGYVAAQAGAVGMILANS 447
Query: 435 EQNGDELMADAHLLPASHVSYTDGESIYQYINSTKTPMAYMTHVRTELGVKPAPFMAAFS 494
E++G+EL+ADAHLLP SH+SY DGE +Y+YINSTKTP+AYMT VRTE G+KPAP MA+FS
Sbjct: 448 EEDGNELIADAHLLPVSHISYIDGEIVYEYINSTKTPVAYMTQVRTETGIKPAPVMASFS 507
Query: 495 SRGPNTIEESILKPDITAPGVNIMAAYSEDTSPSGSPFDNRRIPFNIISGTSMSCPHISG 554
SRGPN+IEESILKPDITAPGVNI+AAYSED SPSGSPFDNRRIPFN++SGTSMSCPHISG
Sbjct: 508 SRGPNSIEESILKPDITAPGVNIIAAYSEDASPSGSPFDNRRIPFNVVSGTSMSCPHISG 567
Query: 555 IVGLLKTLYPQWSPAAIKSAIMTTAETRANDLHPILDTTKVKANPFAYGAGHVRPNRAVN 614
IV LLKT+YP+WSPAAIKSAIMTTAETRANDLHPILD+TK+ ANP AYGAGHV PNRA N
Sbjct: 568 IVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDSTKLVANPLAYGAGHVHPNRAAN 627
Query: 615 PGLVYDLTTKDYLNFLCARGYNETKITKFSNTSFVCSKSFKLTDFNYPSISIPNLKSGPV 674
PGLVYDLTT DYLNFLCARGYN+T+++KFSNTSFVCSKSFKLTDFNYPSISIP +KS PV
Sbjct: 628 PGLVYDLTTNDYLNFLCARGYNKTQLSKFSNTSFVCSKSFKLTDFNYPSISIPKMKSEPV 687
Query: 675 TIKRTVKNVGSPSTYVARVKVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSKNQGY 734
TIKRTVKNVGSPSTYVARVKVPPGV VSV+PSTL+FT DEEKTF+V +SV N+K++GY
Sbjct: 688 TIKRTVKNVGSPSTYVARVKVPPGVLVSVEPSTLKFTRTDEEKTFKVVFRSVTNTKHRGY 747
Query: 735 VFGSLAWSDGKHYVRSPIVVNLG 757
VFGSL W DGKH+VRS IVVNLG
Sbjct: 748 VFGSLKWLDGKHHVRSSIVVNLG 769
BLAST of Sed0018375 vs. ExPASy TrEMBL
Match:
A0A6J1KZG3 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111500243 PE=3 SV=1)
HSP 1 Score: 1199.1 bits (3101), Expect = 0.0e+00
Identity = 579/742 (78.03%), Postives = 668/742 (90.03%), Query Frame = 0
Query: 15 QSYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHINGFAAI 74
+SYIVYLGSHSHGL+PS A L++A+ESHYSLLGS+LGSNE AKEAIFYSYN++INGFAA+
Sbjct: 28 KSYIVYLGSHSHGLNPSSADLQVATESHYSLLGSLLGSNEMAKEAIFYSYNKYINGFAAV 87
Query: 75 LDQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLGVENDDGIPANSIWNLASFGESTII 134
LD V +DLA+HP VVS++ENK RKLHTT+SW FL +EN +G P NSIWN ++FGESTII
Sbjct: 88 LDHKVMQDLARHPAVVSIHENKLRKLHTTNSWEFLELENGEGTPPNSIWNASNFGESTII 147
Query: 135 ANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKFHCNRKLIGARYFNKGYEAIVGSLN 194
ANLDTGVWPESKSFSDEGY IP++W+GSCEGGS FHCN+KLIGARYFNKGY A+ GSL+
Sbjct: 148 ANLDTGVWPESKSFSDEGYEAIPSRWRGSCEGGSNFHCNKKLIGARYFNKGYVALAGSLD 207
Query: 195 ASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKARVVAYKVCWPQQSSG 254
S++TARD +GHGTHTLSTAGG+F+SGANVFG GNGTAKGGSPKARV AYKVCW G
Sbjct: 208 VSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYGNGTAKGGSPKARVAAYKVCW-LTFRG 267
Query: 255 GGCFDADILAAIEAAISDGVDVLSLSLGGNSKEFLDDVTAIGAFHAFQNGIVVVCSAGNS 314
G C DADILAAIEAAI+DGVDVLSLSLG S EF DDV AIGAFHA Q G+VVVC+ GNS
Sbjct: 268 GQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFFDDVIAIGAFHAVQQGMVVVCAGGNS 327
Query: 315 GPALGTVENVAPWLVTVAASTINREFTNYLALGNQKHVKGASLSDKILPTQKFYPLINAA 374
GP L +VENVAPWL TVAASTINR+FT+Y+ALGN+K++ GASLSDKIL Q+FYPLI +A
Sbjct: 328 GPDLQSVENVAPWLFTVAASTINRQFTSYVALGNEKNISGASLSDKILSAQQFYPLITSA 387
Query: 375 DAKANNVSADDARLCQDGMLDPEEVKGKIIVCLRGENGRVDKGYAAAQAGAVGMILANTE 434
DAKA NVS + A+LC +G LDP +VKGKIIVC+RG++ RV+KG+ AA+AGAVGMILAN E
Sbjct: 388 DAKAINVSVEIAKLCVEGSLDPRKVKGKIIVCVRGDDARVNKGFVAAKAGAVGMILANNE 447
Query: 435 QNGDELMADAHLLPASHVSYTDGESIYQYINSTKTPMAYMTHVRTELGVKPAPFMAAFSS 494
+ G++++ADAH+LP SH+SY DGE++Y+YINSTKTP+AYMTHVRTE+G+KPAP MA+FSS
Sbjct: 448 EYGNDVVADAHILPVSHISYVDGETVYEYINSTKTPVAYMTHVRTEMGIKPAPVMASFSS 507
Query: 495 RGPNTIEESILKPDITAPGVNIMAAYSEDTSPSGSPFDNRRIPFNIISGTSMSCPHISGI 554
RGP++IEESILKPDITAPG+NI+AAYS+D SPSGSPFDNRRIPFN++SGTSMSCPHISGI
Sbjct: 508 RGPSSIEESILKPDITAPGINIIAAYSKDASPSGSPFDNRRIPFNLVSGTSMSCPHISGI 567
Query: 555 VGLLKTLYPQWSPAAIKSAIMTTAETRANDLHPILDTTKVKANPFAYGAGHVRPNRAVNP 614
V LLKT+YP+WSPAAIKSAIMTTAETRANDLHPILD TK+ ANP AYGAGHV+PNRA NP
Sbjct: 568 VALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDFTKLVANPLAYGAGHVQPNRAANP 627
Query: 615 GLVYDLTTKDYLNFLCARGYNETKITKFSNTSFVCSKSFKLTDFNYPSISIPNLKSGPVT 674
GLVYDLTT DYLNFLCARGYN+ +++KFSNTSFVCSKSFKLTDFNYPSISIPN+KSGPVT
Sbjct: 628 GLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVCSKSFKLTDFNYPSISIPNMKSGPVT 687
Query: 675 IKRTVKNVGSPSTYVARVKVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSKNQGYV 734
IKRTVKNVGSPSTYVARV+VPPGV VS+KPSTL+FT DEEKTF+V QSVAN+K++GYV
Sbjct: 688 IKRTVKNVGSPSTYVARVEVPPGVLVSIKPSTLKFTRTDEEKTFEVVFQSVANNKHRGYV 747
Query: 735 FGSLAWSDGKHYVRSPIVVNLG 757
FGSL W DGKH+VRS IVVNLG
Sbjct: 748 FGSLKWLDGKHHVRSSIVVNLG 768
BLAST of Sed0018375 vs. ExPASy TrEMBL
Match:
A0A6J1CQB4 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111013335 PE=3 SV=1)
HSP 1 Score: 1184.1 bits (3062), Expect = 0.0e+00
Identity = 573/742 (77.22%), Postives = 655/742 (88.27%), Query Frame = 0
Query: 15 QSYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHINGFAAI 74
+SYI YLGSHSHGL+PS L++A+ESHY+LLGS+LGSN AK+AIFYSYN++INGFAAI
Sbjct: 28 KSYIFYLGSHSHGLNPSAIDLQLATESHYNLLGSLLGSNVAAKDAIFYSYNKYINGFAAI 87
Query: 75 LDQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLGVENDDGIPANSIWNLASFGESTII 134
LD+ VA++LAKHP VVSV+ENK RKLHTT SW FLG+END IP+NSIWNLASFGESTII
Sbjct: 88 LDEKVAQELAKHPSVVSVHENKARKLHTTGSWSFLGLENDGEIPSNSIWNLASFGESTII 147
Query: 135 ANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKFHCNRKLIGARYFNKGYEAIVGSLN 194
NLDTGVWPESKSFSD+GYGPIP++W+GSCEGGSKF CNRKLIGARYFNKGY A +G LN
Sbjct: 148 GNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFRCNRKLIGARYFNKGYVASIGPLN 207
Query: 195 ASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKARVVAYKVCWPQQSSG 254
ASYETARDD+GHGTHTLSTAGGNF+SGA+VFGNGNGTAKGGSP+ARV AY+VCWP +
Sbjct: 208 ASYETARDDEGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVAAYRVCWPAVLT- 267
Query: 255 GGCFDADILAAIEAAISDGVDVLSLSLGGNSKEFLDDVTAIGAFHAFQNGIVVVCSAGNS 314
GGCF ADILA EAAI DGVDVLS+SLGG+ +EF DD AIGAFHA Q+GI VVCSAGNS
Sbjct: 268 GGCFMADILAGFEAAIHDGVDVLSVSLGGSPEEFSDDGLAIGAFHAVQHGITVVCSAGNS 327
Query: 315 GPALGTVENVAPWLVTVAASTINREFTNYLALGNQKHVKGASLSDKILPTQKFYPLINAA 374
GP GTV NVAPW++TV AST +R F +Y+ LGN+KH+KGASLSDKILP QKFYPLI+ A
Sbjct: 328 GPGRGTVSNVAPWMITVGASTADRLFASYVGLGNRKHIKGASLSDKILPAQKFYPLISTA 387
Query: 375 DAKANNVSADDARLCQDGMLDPEEVKGKIIVCLRGENGRVDKGYAAAQAGAVGMILANTE 434
DAKA+NVS + A+LC++G LDP++V+GKI+VCLRG+N RVDKGY AAQAGAVGMILAN +
Sbjct: 388 DAKASNVSLEYAQLCEEGSLDPKKVEGKIVVCLRGDNARVDKGYVAAQAGAVGMILANAQ 447
Query: 435 QNGDELMADAHLLPASHVSYTDGESIYQYINSTKTPMAYMTHVRTELGVKPAPFMAAFSS 494
NGDEL+ADAHLLPASHVSY DGE I+QYI STK PMAYMTHV+TELGVKPAPFMA+FSS
Sbjct: 448 DNGDELLADAHLLPASHVSYADGELIFQYIKSTKIPMAYMTHVKTELGVKPAPFMASFSS 507
Query: 495 RGPNTIEESILKPDITAPGVNIMAAYSEDTSPSGSPFDNRRIPFNIISGTSMSCPHISGI 554
RGPNTIEESILKPDI APGV+I+AAYSE+ SPSGS FD RR PFN SGTSMSCPH+SGI
Sbjct: 508 RGPNTIEESILKPDIIAPGVSIIAAYSEEASPSGSSFDKRRSPFNAESGTSMSCPHVSGI 567
Query: 555 VGLLKTLYPQWSPAAIKSAIMTTAETRANDLHPILDTTKVKANPFAYGAGHVRPNRAVNP 614
VGLLKT YP+WSPAAIKSA+MTTA T+ANDLHPIL+TT++KANP +YGAGHVRPN+A+NP
Sbjct: 568 VGLLKTRYPKWSPAAIKSALMTTAATKANDLHPILNTTQLKANPLSYGAGHVRPNKALNP 627
Query: 615 GLVYDLTTKDYLNFLCARGYNETKITKFSNTSFVCSKSFKLTDFNYPSISIPNLKSGPVT 674
GLVYDLT KDYLNFLCARGYN+T+I KFS+ F CS SFKLTDFNYPSISIPNLK GPV
Sbjct: 628 GLVYDLTLKDYLNFLCARGYNQTQIKKFSSAPFACSHSFKLTDFNYPSISIPNLKFGPVK 687
Query: 675 IKRTVKNVGSPSTYVARVKVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSKNQGYV 734
KR VKNVGSP TYVA+VK PPGV+VSV+P+ L+FTGI EE++F+V ++ V N + +GYV
Sbjct: 688 AKRRVKNVGSPGTYVAQVKAPPGVAVSVEPNMLKFTGIGEEQSFRVVVRRVENEERRGYV 747
Query: 735 FGSLAWSDGKHYVRSPIVVNLG 757
FG LAWSDG H VRSPI VNLG
Sbjct: 748 FGWLAWSDGNHRVRSPIAVNLG 768
BLAST of Sed0018375 vs. TAIR 10
Match:
AT5G59810.1 (Subtilase family protein )
HSP 1 Score: 905.6 bits (2339), Expect = 2.7e-263
Identity = 460/745 (61.74%), Postives = 558/745 (74.90%), Query Frame = 0
Query: 13 LVQSYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHINGFA 72
L +SYIVYLGSH+H S A L + SH + L S +GS+E AKEAIFYSY RHINGFA
Sbjct: 38 LKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFA 97
Query: 73 AILDQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLGVENDDGIPANSIWNLASFGEST 132
AILD+N A ++AKHP VVSV NKGRKLHTT SW F+ + + + +S+WN A +GE T
Sbjct: 98 AILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDT 157
Query: 133 IIANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKFHCNRKLIGARYFNKGYEAIVG- 192
IIANLDTGVWPESKSFSDEGYG +P +WKG C CNRKLIGARYFNKGY A G
Sbjct: 158 IIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTGL 217
Query: 193 SLNASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKARVVAYKVCWPQQ 252
NASYET RD DGHG+HTLSTA GNF+ GANVFG GNGTA GGSPKARV AYKVCWP
Sbjct: 218 PSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWP-P 277
Query: 253 SSGGGCFDADILAAIEAAISDGVDVLSLSLGGNSKEFLDDVTAIGAFHAFQNGIVVVCSA 312
G CFDADILAAIEAAI DGVDVLS S+GG++ +++ D AIG+FHA +NG+ VVCSA
Sbjct: 278 VDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSA 337
Query: 313 GNSGPALGTVENVAPWLVTVAASTINREFTNYLALGNQKHVKGASLSDKILPTQKFYPLI 372
GNSGP GTV NVAPW++TV AS+++REF ++ L N + KG SLS K LP +K Y LI
Sbjct: 338 GNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLS-KPLPEEKMYSLI 397
Query: 373 NAADAKANNVSADDARLCQDGMLDPEEVKGKIIVCLRGENGRVDKGYAAAQAGAVGMILA 432
+AADA N + DA LC+ G LDP++VKGKI+VCLRG+N RVDKG AA AGA GM+L
Sbjct: 398 SAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLC 457
Query: 433 NTEQNGDELMADAHLLPASHVSYTDGESIYQYINSTKTPMAYMTHVRTELGVKPAPFMAA 492
N + +G+E+++DAH+LPAS + Y DGE+++ Y++STK P Y+ L KPAPFMA+
Sbjct: 458 NDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMAS 517
Query: 493 FSSRGPNTIEESILKPDITAPGVNIMAAYSEDTSPSGSPFDNRRIPFNIISGTSMSCPHI 552
FSSRGPNTI ILKPDITAPGVNI+AA++E T P+ DNRR PFN SGTSMSCPHI
Sbjct: 518 FSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHI 577
Query: 553 SGIVGLLKTLYPQWSPAAIKSAIMTTAETRANDLHPILDTTKVKANPFAYGAGHVRPNRA 612
SG+VGLLKTL+P WSPAAI+SAIMTT+ TR N P++D + KANPF+YG+GHV+PN+A
Sbjct: 578 SGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKA 637
Query: 613 VNPGLVYDLTTKDYLNFLCARGYNETKITKFS-NTSFVCSKSFKLTDFNYPSISIPNLKS 672
+PGLVYDLTT DYL+FLCA GYN T + F+ + + C + L DFNYPSI++PNL +
Sbjct: 638 AHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNL-T 697
Query: 673 GPVTIKRTVKNVGSPSTYVARVKVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSKN 732
G +T+ R +KNVG P+TY AR + P GV VSV+P L F E K FQ+ L+ + + +
Sbjct: 698 GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVTPS 757
Query: 733 QGYVFGSLAWSDGKHYVRSPIVVNL 756
GYVFG L W+D HYVRSPIVV L
Sbjct: 758 -GYVFGELTWTDSHHYVRSPIVVQL 776
BLAST of Sed0018375 vs. TAIR 10
Match:
AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 899.0 bits (2322), Expect = 2.5e-261
Identity = 446/743 (60.03%), Postives = 554/743 (74.56%), Query Frame = 0
Query: 16 SYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHINGFAAIL 75
SY+VY G+HSH + ++ E+HY LGS GS E A +AIFYSY +HINGFAA L
Sbjct: 31 SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90
Query: 76 DQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLGVENDDGIPANSIWNLASFGESTIIA 135
D ++A +++KHP VVSV NK KLHTT SW FLG+E++ +P++SIW A FGE TIIA
Sbjct: 91 DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150
Query: 136 NLDTGVWPESKSFSDEGYGPIPTKWKGSCEG--GSKFHCNRKLIGARYFNKGYEAIVGSL 195
NLDTGVWPESKSF DEG GPIP++WKG C+ + FHCNRKLIGARYFNKGY A VG L
Sbjct: 151 NLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHL 210
Query: 196 NASYETARDDDGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKARVVAYKVCWPQQSS 255
N+S+++ RD DGHG+HTLSTA G+F+ G ++FG GNGTAKGGSP+ARV AYKVCWP
Sbjct: 211 NSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWP-PVK 270
Query: 256 GGGCFDADILAAIEAAISDGVDVLSLSLGGNSKEFLDDVTAIGAFHAFQNGIVVVCSAGN 315
G C+DAD+LAA +AAI DG DV+S+SLGG F +D AIG+FHA + IVVVCSAGN
Sbjct: 271 GNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGN 330
Query: 316 SGPALGTVENVAPWLVTVAASTINREFTNYLALGNQKHVKGASLSDKILPTQKFYPLINA 375
SGPA TV NVAPW +TV AST++REF + L LGN KH KG SLS LP KFYP++ +
Sbjct: 331 SGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMAS 390
Query: 376 ADAKANNVSADDARLCQDGMLDPEEVKGKIIVCLRGENGRVDKGYAAAQAGAVGMILANT 435
+AKA N SA DA+LC+ G LDP + KGKI+VCLRG+NGRV+KG A A G +GM+L NT
Sbjct: 391 VNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENT 450
Query: 436 EQNGDELMADAHLLPASHVSYTDGESIYQYINSTKTPMAYMTHVRTELGVKPAPFMAAFS 495
G++L+AD H+LPA+ ++ D ++ +YI+ TK P+A++T RT+LG+KPAP MA+FS
Sbjct: 451 YVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFS 510
Query: 496 SRGPNTIEESILKPDITAPGVNIMAAYSEDTSPSGSPFDNRRIPFNIISGTSMSCPHISG 555
S+GP+ + ILKPDITAPGV+++AAY+ SP+ FD RR+ FN ISGTSMSCPHISG
Sbjct: 511 SKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISG 570
Query: 556 IVGLLKTLYPQWSPAAIKSAIMTTAETRANDLHPILDTTKVKANPFAYGAGHVRPNRAVN 615
I GLLKT YP WSPAAI+SAIMTTA + PI + T +KA PF++GAGHV+PN AVN
Sbjct: 571 IAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVN 630
Query: 616 PGLVYDLTTKDYLNFLCARGYNETKITKFSNTSFVCSK-SFKLTDFNYPSISIPNLKSGP 675
PGLVYDL KDYLNFLC+ GYN ++I+ FS +F CS L + NYPSI++PNL S
Sbjct: 631 PGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSK 690
Query: 676 VTIKRTVKNVGSPSTYVARVKVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSKNQG 735
VT+ RTVKNVG PS Y +V P GV V+VKP++L FT + E+KTF+V L + +G
Sbjct: 691 VTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKG 750
Query: 736 YVFGSLAWSDGKHYVRSPIVVNL 756
YVFG L WSD KH VRSPIVV L
Sbjct: 751 YVFGELVWSDKKHRVRSPIVVKL 772
BLAST of Sed0018375 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 646.4 bits (1666), Expect = 2.9e-185
Identity = 347/736 (47.15%), Postives = 470/736 (63.86%), Query Frame = 0
Query: 28 LHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHINGFAAILDQNVAEDLAKHP 87
+H + + + + + H + S L S ++ E + Y+Y I+GF+ L Q A+ L P
Sbjct: 34 VHMAKSQMPSSFDLHSNWYDSSLRSISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQP 93
Query: 88 GVVSVNENKGRKLHTTSSWRFLGVENDDGIPANSIWNLASFGESTIIANLDTGVWPESKS 147
GV+SV +LHTT + FLG++ ++ A ++ LDTGVWPESKS
Sbjct: 94 GVISVLPEHRYELHTTRTPLFLGLDEHTA----DLFPEAGSYSDVVVGVLDTGVWPESKS 153
Query: 148 FSDEGYGPIPTKWKGSCEGGSKFH---CNRKLIGARYFNKGYEAIVGSLNASYE--TARD 207
+SDEG+GPIP+ WKG CE G+ F CNRKLIGAR+F +GYE+ +G ++ S E + RD
Sbjct: 154 YSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRD 213
Query: 208 DDGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKARVVAYKVCWPQQSSGGGCFDADI 267
DDGHGTHT STA G+ + GA++ G +GTA+G +P+ARV YKVCW GGCF +DI
Sbjct: 214 DDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL-----GGCFSSDI 273
Query: 268 LAAIEAAISDGVDVLSLSLGGNSKEFLDDVTAIGAFHAFQNGIVVVCSAGNSGPALGTVE 327
LAAI+ AI+D V+VLS+SLGG ++ D AIGAF A + GI+V CSAGN+GP+ ++
Sbjct: 274 LAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLS 333
Query: 328 NVAPWLVTVAASTINREFTNYLALGNQKHVKGASLSDKILPTQKFYPLINAADAKANNVS 387
NVAPW+ TV A T++R+F LGN K+ G SL K P I A +A +
Sbjct: 334 NVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNAS----N 393
Query: 388 ADDARLCQDGMLDPEEVKGKIIVCLRGENGRVDKGYAAAQAGAVGMILANTEQNGDELMA 447
A + LC G L PE+VKGKI++C RG N RV KG AG VGMILANT NG+EL+A
Sbjct: 394 ATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVA 453
Query: 448 DAHLLPASHVSYTDGESIYQYINSTKTPMAYMTHVRTELGVKPAPFMAAFSSRGPNTIEE 507
DAHLLPA+ V G+ I Y+ + P A ++ + T +GVKP+P +AAFSSRGPN+I
Sbjct: 454 DAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITP 513
Query: 508 SILKPDITAPGVNIMAAYSEDTSPSGSPFDNRRIPFNIISGTSMSCPHISGIVGLLKTLY 567
+ILKPD+ APGVNI+AA++ P+G D+RR+ FNIISGTSMSCPH+SG+ LLK+++
Sbjct: 514 NILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVH 573
Query: 568 PQWSPAAIKSAIMTTAETRANDLHPILD-TTKVKANPFAYGAGHVRPNRAVNPGLVYDLT 627
P+WSPAAI+SA+MTTA D P+LD T + PF +GAGHV P A NPGL+YDLT
Sbjct: 574 PEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLT 633
Query: 628 TKDYLNFLCARGYNETKITKFSNTSFVC--SKSFKLTDFNYPSISIPNLKSGPVTIKRTV 687
T+DYL FLCA Y +I S ++ C SKS+ + D NYPS ++ G RTV
Sbjct: 634 TEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTV 693
Query: 688 KNVGSPSTYVARV-KVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSKNQGYVFGSL 747
+VG TY +V GV +SV+P+ L F +E+K++ V ++ + FGS+
Sbjct: 694 TSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSI 753
Query: 748 AWSDGKHYVRSPIVVN 755
WSDGKH V SP+ ++
Sbjct: 754 EWSDGKHVVGSPVAIS 755
BLAST of Sed0018375 vs. TAIR 10
Match:
AT5G45650.1 (subtilase family protein )
HSP 1 Score: 590.5 bits (1521), Expect = 1.9e-168
Identity = 340/779 (43.65%), Postives = 463/779 (59.44%), Query Frame = 0
Query: 15 QSYIVYLGSHSHGLHPSPASLRIASESHYSLLGSMLGSNEEAKEAIFYSYNRHINGFAAI 74
Q YIVY G H + E H+S L S+ S E+A+ ++ YSY INGFAA
Sbjct: 25 QVYIVYFGEHK-----GDKAFHEIEEHHHSYLQSVKESEEDARASLLYSYKHSINGFAAE 84
Query: 75 LDQNVAEDLAKHPGVVSVNENKGRK--LHTTSSWRFLGV---ENDDGIP----------- 134
L + A L K VVSV ++ RK HTT SW F+G+ E D +P
Sbjct: 85 LTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDVPRRKNDADDRFR 144
Query: 135 -ANSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKF---HCNR 194
+ A G+ I+ LD+GVWPESKSF+D+G GP+P WKG C+ G F HCNR
Sbjct: 145 VGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGICQTGVAFNSSHCNR 204
Query: 195 KLIGARYFNKGYEAIVGSLNAS----YETARDDDGHGTHTLSTAGGNFISGANVFGN-GN 254
K+IGARY+ KGYE G+ NA+ + + RD DGHG+HT STA G + GA+ G
Sbjct: 205 KIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGRRVLGASALGGFAK 264
Query: 255 GTAKGGSPKARVVAYKVCWPQQSS----GGGCFDADILAAIEAAISDGVDVLSLSLGGNS 314
G+A GG+P AR+ YK CW + ++ G C + D+LAAI+ AI+DGV V+S+S+G
Sbjct: 265 GSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADGVHVISISIGTTE 324
Query: 315 K-EFLDDVTAIGAFHAFQNGIVVVCSAGNSGPALGTVENVAPWLVTVAASTINREFTNYL 374
F D A+GA HA + IVV SAGNSGP GT+ N+APW++TV AST++R F L
Sbjct: 325 PFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVGASTLDRAFVGGL 384
Query: 375 ALGNQKHVKGASLSDKILPTQKFYPLINAADAKANNVSADDARLCQDGMLDPEEVKGKII 434
LGN +K S++ KF PL+ A++ ++ ++ C L PE V GK++
Sbjct: 385 VLGNGYTIKTDSIT--AFKMDKFAPLVYASNVVVPGIALNETSQCLPNSLKPELVSGKVV 444
Query: 435 VCLRGENGRVDKGYAAAQAGAVGMILANTEQNGDELMADAHLLPASHVSYTDGESIYQYI 494
+CLRG R+ KG +AG GMIL N NG+E+ +D+H +P + V+ T + I +YI
Sbjct: 445 LCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPTAGVTPTVVDKILEYI 504
Query: 495 NSTKTPMAYMTHVRTELGVKPAPFMAAFSSRGPNTIEESILKPDITAPGVNIMAAYSEDT 554
+ K P A++ +T + AP M FSSRGPN ++ +ILKPDITAPG+ I+AA+S
Sbjct: 505 KTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDITAPGLYILAAWSGAD 564
Query: 555 SPSGSPFDNRRIPFNIISGTSMSCPHISGIVGLLKTLYPQWSPAAIKSAIMTTAETRAND 614
SPS D R +NI SGTSMSCPH++G + LLK ++P+WS AAI+SA+MTTA +
Sbjct: 565 SPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSSAAIRSALMTTAWMTNDK 624
Query: 615 LHPILDTTKVKANPFAYGAGHVRPNRAVNPGLVYDLTTKDYLNFLCARGYNETKITKFSN 674
PI DTT + ANPFA G+GH RP +A +PGLVYD + + YL + C+ IT
Sbjct: 625 KKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYLLYGCS-----VNITNIDP 684
Query: 675 TSFVCSKSFKLTDFNYPSISIPNLKSGPVTIKRTVKNVG---SPSTYVARVKVPPGVSVS 734
T SK + NYPSI++PNLK VT+KRTV NVG S STY+ VK P G+SV
Sbjct: 685 TFKCPSKIPPGYNHNYPSIAVPNLKK-TVTVKRTVTNVGTGNSTSTYLFSVKPPSGISVK 744
Query: 735 VKPSTLEFTGIDEEKTFQVELQ----SVANSKNQG-YVFGSLAWSDGKHYVRSPIVVNL 756
P+ L F I +++ F++ ++ V N+ +G Y FG +W+D H VRSPI V+L
Sbjct: 745 AIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWFSWTDKVHVVRSPIAVSL 790
BLAST of Sed0018375 vs. TAIR 10
Match:
AT4G34980.1 (subtilisin-like serine protease 2 )
HSP 1 Score: 583.2 bits (1502), Expect = 3.1e-166
Identity = 326/727 (44.84%), Postives = 460/727 (63.27%), Query Frame = 0
Query: 52 SNEEAKEA-IFYSYNRHINGFAAILDQNVAEDLAKHPGVVSVNENKGRKLHTTSSWRFLG 111
S E A+E+ I + Y+ +GF+A++ + A++L HP V++V E++ R+LHTT S +FLG
Sbjct: 49 STEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLG 108
Query: 112 VENDDGIPANSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPTKWKGSCEGGSKF 171
++N G +W+ + +G II DTG+WPE +SFSD GPIP +W+G CE G++F
Sbjct: 109 LQNQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARF 168
Query: 172 ---HCNRKLIGARYFNKGYE-AIVGSLNASYE--TARDDDGHGTHTLSTAGGNFISGANV 231
+CNRK+IGAR+F KG + A++G +N + E + RD DGHGTHT STA G A++
Sbjct: 169 SPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASM 228
Query: 232 FGNGNGTAKGGSPKARVVAYKVCWPQQSSGGGCFDADILAAIEAAISDGVDVLSLSLGG- 291
G +G AKG +PKAR+ AYKVCW GC D+DILAA +AA+ DGVDV+S+S+GG
Sbjct: 229 SGYASGVAKGVAPKARIAAYKVCW----KDSGCLDSDILAAFDAAVRDGVDVISISIGGG 288
Query: 292 ---NSKEFLDDVTAIGAFHAFQNGIVVVCSAGNSGPALGTVENVAPWLVTVAASTINREF 351
S +LD + AIG++ A GI V SAGN GP +V N+APW+ TV ASTI+R F
Sbjct: 289 DGITSPYYLDPI-AIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNF 348
Query: 352 TNYLALGNQKHVKGASLSDKILPTQKFYPLINAADAKANNVSADDARLCQDGMLDPEEVK 411
LG+ ++G SL + + +P++ + ++ S LC + LDP++V+
Sbjct: 349 PADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPKQVR 408
Query: 412 GKIIVCLRGENGRVDKGYAAAQAGAVGMILANTEQNGDELMADAHLLPASHVSYTDGESI 471
GKI++C RG + RV KG +AG VGMILAN NG+ L+ DAHL+PA V +G+ I
Sbjct: 409 GKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRI 468
Query: 472 YQYINSTKTPMAYMTHVRTELGVKPAPFMAAFSSRGPNTIEESILKPDITAPGVNIMAAY 531
Y +S P+A + T +G+KPAP +A+FS RGPN + ILKPD+ APGVNI+AA+
Sbjct: 469 KAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAW 528
Query: 532 SEDTSPSGSPFDNRRIPFNIISGTSMSCPHISGIVGLLKTLYPQWSPAAIKSAIMTTAET 591
++ P+G P D R+ FNI+SGTSM+CPH+SG LLK+ +P WSPA I+SA+MTT
Sbjct: 529 TDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNL 588
Query: 592 RANDLHPILD-TTKVKANPFAYGAGHVRPNRAVNPGLVYDLTTKDYLNFLCARGYNETKI 651
N ++D +T A P+ YG+GH+ RA+NPGLVYD+T DY+ FLC+ GY I
Sbjct: 589 VDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTI 648
Query: 652 TKFSNTSFVCSKSFKLT--DFNYPSIS--IPNLKSGPV--TIKRTVKNVG-SPSTYVARV 711
+ T C + K + + NYPSI+ P + G V T+ RT NVG + + Y AR+
Sbjct: 649 QVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARI 708
Query: 712 KVPPGVSVSVKPSTLEFTGIDEEKTFQVELQSVANSKN-----QGYVFGSLAWSD-GKHY 754
+ P GV+V+VKP L FT + +++ V + N++N G VFGS+ W D GKH
Sbjct: 709 ESPRGVTVTVKPPRLVFTSAVKRRSYAVTV--TVNTRNVVLGETGAVFGSVTWFDGGKHV 758
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_016900594.1 | 0.0e+00 | 84.61 | PREDICTED: subtilisin-like protease SBT5.3, partial [Cucumis melo] | [more] |
| XP_038904203.1 | 0.0e+00 | 83.79 | uncharacterized protein LOC120090550 [Benincasa hispida] | [more] |
| TYK19383.1 | 0.0e+00 | 81.18 | subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa] | [more] |
| XP_023553316.1 | 0.0e+00 | 77.57 | subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo] | [more] |
| XP_022932414.1 | 0.0e+00 | 79.54 | subtilisin-like protease SBT5.4 [Cucurbita moschata] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| F4JXC5 | 3.8e-262 | 61.74 | Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... | [more] |
| Q9ZSP5 | 3.5e-260 | 60.03 | Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... | [more] |
| I1N462 | 1.5e-218 | 54.17 | Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... | [more] |
| O65351 | 4.1e-184 | 47.15 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| Q9FK76 | 2.7e-167 | 43.65 | Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A1S4DX85 | 0.0e+00 | 84.61 | subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491136 PE=3 SV=... | [more] |
| A0A5D3D7B7 | 0.0e+00 | 81.18 | Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... | [more] |
| A0A6J1EWX5 | 0.0e+00 | 79.54 | subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111438769 PE... | [more] |
| A0A6J1KZG3 | 0.0e+00 | 78.03 | subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111500243 PE=3... | [more] |
| A0A6J1CQB4 | 0.0e+00 | 77.22 | subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111013335 P... | [more] |
