Sed0018061 (gene) Chayote v1

BLAST of Sed0018061 vs. NCBI nr
Match: KAG7029801.1 ((R)-mandelonitrile lyase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1511.9 bits (3913), Expect = 0.0e+00
Identity = 733/1019 (71.93%), Postives = 857/1019 (84.10%), Query Frame = 0

Query: 1    MKFVHDANEFPAKEVYDYIVIGGGTAGCPLATTLSSKFSVLILETGSDPNKYPSVLSEQG 60
            MKFV + N  P +E YDYI+IGGGTAGCPLA TLSS FSVL+LE GSDPN +PSVLS+QG
Sbjct: 1    MKFVQNGNTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQG 60

Query: 61   LLNAFAAKDDGINPFNRFISEDGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVNWDM 120
            L N     DDG+NPF RF+SEDGVENIRGRVLGG SM+N GFYSR   EFF+++G+ WDM
Sbjct: 61   LANTLTDNDDGLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQWDM 120

Query: 121  EMVEEAYEWVEESVVSRPSLNAWQSAFRSSLLESGVVPDNGFSLKHIVGTKTSGSIFDGK 180
              VE+AY+W+EE+VVSRP L+ WQSAFR +LLE+GV PDNG+ LKH VGT+T GSIFD +
Sbjct: 121  TAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIFDSR 180

Query: 181  GNRHGAVELLNKAEPRNLKIAIRATVQRIIFS---GLSAIGVLYSDSKGKLHKAFIRNKG 240
            G RHGAVELLNKA+PRNL++A +ATV+RIIFS   GLSA GVLYSD KGKLHKA I   G
Sbjct: 181  GRRHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLSASGVLYSDFKGKLHKATISKNG 240

Query: 241  EIIVSAGALGSPQLLLLSGIGPKSHLSSLKLPVVLHQRHVGQFMSDNPRFTSSIVLPFPV 300
            EII++AGA+GSP LLL SG+GPKSHLSSLKLPVVLH RHVGQ M+DNPRF ++IVLPF +
Sbjct: 241  EIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVLPF-L 300

Query: 301  LSQTSAKVVGILENNIYLQSFASSLPFSFPPSFSLLPPKSNSVNMTLAIVAGKFSTVDSV 360
               TS +VVG L+ NI+++S +S LPFS  P F LLPP+S +VN++LA+ AGKFSTV SV
Sbjct: 301  TPPTSVQVVGTLKRNIHIESLSSILPFSIFPPFGLLPPRSTAVNLSLAVFAGKFSTVSSV 360

Query: 361  GSLRLTSSVNAKKNPIVRFNYYSHPDDVSRCVRGVRKIGDLLRTETMERIKTRDLEGKKG 420
            GSLRL    + +KNPIVRFNY SHPDDV RCV GVRK+GDL+ T++MERIKT DLEGKKG
Sbjct: 361  GSLRL----DGRKNPIVRFNYLSHPDDVERCVEGVRKVGDLVNTKSMERIKTGDLEGKKG 420

Query: 421  FRFLGPALPSNLSDYSSVREFCRETVTSYWHYHGGCLVGKVVDGDYKVMGIKNLRVVDGS 480
            F+FLG  LP N+SDY  V +FCR+TVT++WHYHGGCLVGKVVD +Y+V+GIK LRVVDGS
Sbjct: 421  FKFLGAPLPENMSDYKLVGDFCRKTVTTFWHYHGGCLVGKVVDDNYRVIGIKKLRVVDGS 480

Query: 481  TFSESPGTNPMATVMMLGR--------------YMKFVHDANEFPEKEEYDYIVIGGGTA 540
            TFS SPGTNPMATVMMLGR              YMKFVH+A + PEK+EYDYI+IGGG A
Sbjct: 481  TFSLSPGTNPMATVMMLGRYVGLKMLQQRLDVSYMKFVHNAGDLPEKQEYDYIIIGGGAA 540

Query: 541  GCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQGLFNAFATKDDGENPFQRFVSEEGVEN 600
            GCPLAAT+SSKF VLLLERGS+PNKYPSVLNEQGL NAF  +DDG+NPFQRF SE+GVEN
Sbjct: 541  GCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQGLLNAFLAQDDGQNPFQRFTSEDGVEN 600

Query: 601  IRGRVLGGSSMLNAGFYSRGHREFFETAGVEWDMEMVEKAYEWVEETVVSEPSLNAWHSA 660
            IRGR+LGG +M+NAGFYSRGHR+FFETAGV WDMEMVE AY+WVEETVVS+P LNAW SA
Sbjct: 601  IRGRILGGGTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQWVEETVVSKPILNAWQSA 660

Query: 661  FRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDGEGNRHGAVELLNKAKPTNLKIAIRATV 720
            F+SALL++G+ PDNGF+L HL+GTKTGGSIFDG+GNRHGAVELLNKA+P NLK+A+ ATV
Sbjct: 661  FKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDGKGNRHGAVELLNKAEPKNLKVAVNATV 720

Query: 721  ERIIFSDLSASGVLYSDSKGRLHKALIRNKGEIMVSAGAMGSPQLLLLSGVGPKSHLSSL 780
             +I+F+ LSA+GV YSDSKG++H A IR KGEI +SAGA+GSP LLL SGVGPKSHLSSL
Sbjct: 721  RKILFNGLSANGVSYSDSKGKIHTAFIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSL 780

Query: 781  KLPVVLDQQYVGQFLSDNPRFSANIVLPFPLLPTSGKVVGILDDNIYFQSFAGSLPFSLP 840
            KLPVV  Q YVG+F+SDNPRF A IVLPF L  +S KVVG L DN++ Q+FA   PF  P
Sbjct: 781  KLPVVHHQPYVGEFMSDNPRFGATIVLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLAP 840

Query: 841  SSFSLLPPRSDFVDMSLAIFFGKFSKVDSVGSLRLNSSTDVKKSPLVRFNYYSHPDDLAR 900
             +FSLLPP++  ++ SL IF GKFS+V S G LRLNS+TD K + +VRFNYYSHPDDLAR
Sbjct: 841  PTFSLLPPQATSINPSLVIFVGKFSEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLAR 900

Query: 901  CVRGVRKMGDLLQTKTMEKIKTIDLEGKKGFRFLGSPLPENLSDYSSVGQFCREILATYW 960
            CV GVRK+GDLL+T+TMEKIKT DLEG KGF+F+G PLPENL D SSV ++CR+ + TYW
Sbjct: 901  CVEGVRKVGDLLKTQTMEKIKTQDLEGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYW 960

Query: 961  HYHGGCLVGKVVDGDYKVMGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLKER 1003
            HYHGGCLVGKVVDGDY+V+G+KNLRVVDGSTFS+SPGTNPMATLMMLGRYVGLKVL+ER
Sbjct: 961  HYHGGCLVGKVVDGDYRVIGMKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQER 1014

BLAST of Sed0018061 vs. NCBI nr
Match: KAF4383855.1 (hypothetical protein G4B88_016288 [Cannabis sativa])

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 563/1037 (54.29%), Postives = 735/1037 (70.88%), Query Frame = 0

Query: 1    MKFVHDANEFPAKEVYDYIVIGGGTAGCPLATTLSSKFSVLILETGSDPNKYPSVLSEQG 60
            MK V++A + P +E YDYIV+GGGTAGCPLA TLS K+SVL+LE G+ P  +P+ L   G
Sbjct: 35   MKSVYNATDMPLEEEYDYIVVGGGTAGCPLAATLSEKYSVLVLERGNVPKAHPNTLVASG 94

Query: 61   LLNAF--AAKDDGINPFNRFISEDGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVNW 120
             L     A  DDG  P  RF SE+GVE++RGRVLGG+SM+NA F+S    +F   +GV W
Sbjct: 95   FLTNLVEADDDDGDTPAQRFTSEEGVESVRGRVLGGTSMINAAFFSEADNDFLAKSGVEW 154

Query: 121  DMEMVEEAYEWVEESVVSRPSLNAWQSAFRSSLLESGVVPDNGFSLKHIVGTKTSGSIFD 180
            D + VE+AYEWV+  +VS  +L+ WQSA + +LLE+GV PDNG + KH VGTK SGS FD
Sbjct: 155  DNDEVEKAYEWVKSKIVSYSNLSTWQSAVKEALLEAGVGPDNGVTTKHKVGTKQSGSTFD 214

Query: 181  GKGNRHGAVELLNKAEPRNLKIAIRATVQRIIF----SGLSAIGVLYSDSKGKLHKAFIR 240
              G RHGAVELLNK   +N+KIAI A V++IIF    S  SAIGV+Y+DSKGK HKA IR
Sbjct: 215  NMGRRHGAVELLNKGNLKNMKIAIHAYVKKIIFSTKSSNPSAIGVIYTDSKGKYHKALIR 274

Query: 241  NKGEIIVSAGALGSPQLLLLSGIGPKSHLSSLKLPVVLHQRHVGQFMSDNPRFTSSIVLP 300
            NKGE+I+SAGALGSPQLLLLSGIGPKS+LSS  +P+V  Q +VG+FM+DNPR   ++++P
Sbjct: 275  NKGEVILSAGALGSPQLLLLSGIGPKSYLSSHHIPIVHSQSNVGKFMADNPRNNINLIIP 334

Query: 301  FPVLSQTSAKVVGI-----LENNIYLQSFA-SSLPFSFPPSFSLLPPKSNSVNMTLAIVA 360
            FP    + A+VVGI     +E   Y   FA ++LPFSF P+  L PP+      +LA + 
Sbjct: 335  FP-FEGSGAQVVGITDDYFIETVSYSLPFAPTTLPFSFYPN-PLTPPQ-----FSLASIV 394

Query: 361  GKFSTVDSVGSLRLTSSVNAKKNPIVRFNYYSHPDDVSRCVRGVRKIGDLLRTETMERIK 420
             K     S GSL L S+ + K  P VRFNY+S+P D++RCV  +RK+G++L T++++R K
Sbjct: 395  EKIVGPLSTGSLTLASTKDVKITPHVRFNYFSNPIDLARCVSAMRKVGEMLETKSLDRFK 454

Query: 421  TRDLEGKKGFRFLGPALPSNLSDYSSVREFCRETVTSYWHYHGGCLVGKVVDGDYKVMGI 480
             +DL G + F +LGP LP+N SD S++ ++CR +VT++WHYHGGCLVGKVVDGD+KV+G 
Sbjct: 455  YKDLNGARDFMYLGPPLPANQSDNSAMEDYCRSSVTTFWHYHGGCLVGKVVDGDFKVIGT 514

Query: 481  KNLRVVDGSTFSESPGTNPMATVMMLGR----------------------YMKFVHDANE 540
             +LRVVDGSTF  SPGTNP AT+MM+GR                      YMK V++A +
Sbjct: 515  NSLRVVDGSTFVISPGTNPQATLMMIGRVGDNNGPFGLGIAVSGADYDFSYMKSVYNATD 574

Query: 541  FPEKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQG-LFNAFATK 600
             P +EEYDYIV+GGGTAGCPLAAT+S K+SVL+LERG+ P  +P+ L   G L N     
Sbjct: 575  MPLEEEYDYIVVGGGTAGCPLAATLSEKYSVLVLERGNVPKAHPNTLVASGFLTNLIEAD 634

Query: 601  DDGENPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWDMEMVEKAYE 660
            DDG+ P QRF S EGVEN+RGRVLGG+SM+NA F+S    +F   +GV+WD + VEKAYE
Sbjct: 635  DDGDTPAQRFTS-EGVENVRGRVLGGTSMINAAFFSEADNDFMVKSGVKWDNDEVEKAYE 694

Query: 661  WVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDGEGNRHGAVE 720
            WV E++VS  +++ W  A + ALL++G+ PDNG + +H +GTK  GS FD  G RHGAVE
Sbjct: 695  WVRESIVSFSNMSTWQFAVKEALLEAGVGPDNGVTTKHKIGTKQSGSTFDNMGRRHGAVE 754

Query: 721  LLNKAKPTNLKIAIRATVERIIFSDLSAS------GVLYSDSKGRLHKALIRNKGEIMVS 780
            LLNK    N+KIAI A V ++IFS  S+S      GV+Y+DSKG+ HKALIRNKGE+++S
Sbjct: 755  LLNKGDLQNMKIAIHAYVNKVIFSTKSSSSNPSAIGVIYTDSKGKSHKALIRNKGEVILS 814

Query: 781  AGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSANIVLPFPLLPTSG 840
            AGA+GSPQLLLLSG+GPKS+LSS  +P++  Q  VGQF++DNPR + N+++PFPL  ++ 
Sbjct: 815  AGALGSPQLLLLSGIGPKSYLSSQHIPIIHSQSNVGQFMADNPRNNINLIIPFPLEGSAV 874

Query: 841  KVVGILDDNIYFQSFAGSLPFSLPS-SFSLLPPRSDFVDMSLAIFFGKFSKVDSVGSLRL 900
            +VVGI +D  + ++ + SLPF+  +  FS  P        SLA    K     S GSL L
Sbjct: 875  QVVGITND-YFIETVSYSLPFAPTTLPFSFYPNPLTQPQFSLATIAAKIVGPLSTGSLTL 934

Query: 901  NSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIKTIDLEGKKGFRFLG 960
             S+ DVK +P VRFNY+S+P DLARCV  +RK+G++L+TK++++ K  DL G + F FLG
Sbjct: 935  ASTKDVKITPHVRFNYFSNPIDLARCVSAMRKLGEMLETKSLDRFKYKDLNGARDFMFLG 994

Query: 961  SPLPENL-SDYSSVGQFCREILATYWHYHGGCLVGKVVDGDYKVMGIKNLRVVDGSTFSE 995
              LP N  S+ S +  +CR  + T WHYHGGCLVGKVVDGD+KV+G  +LRVVDGSTF  
Sbjct: 995  PTLPSNYQSNNSGMEDYCRSSVTTIWHYHGGCLVGKVVDGDFKVIGTNSLRVVDGSTFVI 1054

BLAST of Sed0018061 vs. NCBI nr
Match: KAF4349168.1 (hypothetical protein F8388_026317 [Cannabis sativa])

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 569/1116 (50.99%), Postives = 733/1116 (65.68%), Query Frame = 0

Query: 1    MKFVHDANEFP-AKEVYDYIVIGGGTAGCPLATTLSSKFSVLILETGSDPNKYPSVLSEQ 60
            MK VHDA E    +E YDYI+IGGGTAGCPLA TLS  +SVL+LE GS P   P+VL   
Sbjct: 43   MKSVHDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTANPNVLHLH 102

Query: 61   GLLNAFAAKDD--GINPFNRFISEDGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVN 120
            G       +++   + P  RF SEDGVEN RGRVLGG+SM+NAGF+SRG   FF   GV 
Sbjct: 103  GFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGFFSKPGVK 162

Query: 121  WDMEMVEEAYEWVEESVVSRPSLNAWQSAFRSSLLESGVVPDNGFSLKHIVGTKTSGSIF 180
            W+M+ VE+AYEWVEES+V RP L  WQS+FR +LLE GV PDN F L H +GTK SGS F
Sbjct: 163  WEMDRVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGTKISGSTF 222

Query: 181  DGKGNRHGAVELLNKAEPRNLKIAIRATVQRIIFSGL--------------------SAI 240
            D  G RHGAVELLNK    NLK+ + ATV++I FS                       AI
Sbjct: 223  DEVGRRHGAVELLNKGNLNNLKVVVHATVEKIFFSTKVSRNYHSGDDNDHDNNPPKPYAI 282

Query: 241  GVLYSDSKGKLHKAFIRNKGEIIVSAGALGSPQLLLLSGIGPKSHLSSLKLPVVLHQRHV 300
            GV+YSDSKGK H   +++KGE+I+SAGA+GSPQLLLLSGIGP S+LSSL +P+V  Q +V
Sbjct: 283  GVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVHSQPNV 342

Query: 301  GQFMSDNPRFTSSIVLPFPVLSQTSAKVVGILENNIYLQSFASSLPFSFPPSFSLLPPKS 360
            G F++DNPR   ++++P P    +  +VVGI  N+ ++++ +++LP S  P    + PKS
Sbjct: 343  GDFIADNPRNNINLIIPSPT-DPSPVQVVGI-TNHYFIETISANLPASLTPLPFSVYPKS 402

Query: 361  NSVNMTLAIVAGKFSTVDSVGSLRLTSSVNAKKNPIVRFNYYSHPDDVSRCVRGVRKIGD 420
            ++ ++ + ++  K     S GSL L S  + +  P VRFNY+SHP D+S+CV GVRKIG+
Sbjct: 403  STAHLGMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVNGVRKIGE 462

Query: 421  LLRTETMERIKTRDLEGKKGFRFLGPALPSNLSDYSSVREFCRETVTSYWHYHGGCLVGK 480
            +L T  MER K  D   ++ F + GP+LP+N SD S++ EFCR +VT+ WHYHGGC VGK
Sbjct: 463  MLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGK 522

Query: 481  VVDGDYKVMGIKNLRVVDGSTFSESPGTNPMATVMMLG---------------------- 540
            VVDGD++VMG+ +LRVVDGSTF  SPGTNP ATVMMLG                      
Sbjct: 523  VVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQEREAEANVEGDIE 582

Query: 541  ---------------------------------------------RYMKFVHDANEFP-E 600
                                                         RYMK V+DA E   E
Sbjct: 583  RREIDEKDRVKDYLSTVALRVSRWATSDGQQQVLALATSGSDHDFRYMKSVYDATEMSLE 642

Query: 601  KEEYDYIVIGGG--TAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQG-LFNAFATKD 660
            +E YDYI+IGGG  TAGCPLAAT+S  +SVL+LERGS P   P+VL+  G L N    ++
Sbjct: 643  EEYYDYIIIGGGTLTAGCPLAATLSENYSVLVLERGSVPTSNPNVLHLSGFLANLMQEEE 702

Query: 661  DGE-NPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWDMEMVEKAYE 720
            +    P QRF SE+GVEN+RGRVLGGSSM+NAGF+SRG   F+  +GV+W+M+ VEKAYE
Sbjct: 703  ETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSRGDEGFYSKSGVKWEMDRVEKAYE 762

Query: 721  WVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDGEGNRHGAVE 780
            WVEE++V  P L  W S+FR ALL+ G+ P+NGF L H +GTK  GS FD  G RHGAVE
Sbjct: 763  WVEESIVFRPKLPVWQSSFRDALLEVGVGPNNGFDLNHKLGTKISGSTFDEVGRRHGAVE 822

Query: 781  LLNKAKPTNLKIAIRATVERIIFSDL------------------SASGVLYSDSKGRLHK 840
            LLNK    NLK+ + ATV+RIIFS                     A GV+YSDSKG+ H 
Sbjct: 823  LLNKGNLNNLKVGVHATVDRIIFSTKVSNNYHSGDDSDQNSPKPYAIGVMYSDSKGKSHT 882

Query: 841  ALIRNKGEIMVSAGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSAN 900
             L+R+KGEI++SAGA+GSPQLLLLSG+GP S+LSSL +P+VL Q  VG F++DNPR + N
Sbjct: 883  VLVRHKGEIILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIADNPRNNIN 942

Query: 901  IVLPFPLLPTSGKVVGILDDNIYFQSFAGSLPFS-LPSSFSLLPPRSDFVDMSLAIFFGK 960
            +++PFP  P++ +VVGI  D+ + ++ + +LP S  P  FS+  P+S   ++ +AI   K
Sbjct: 943  LIIPFPTEPSAVQVVGI-TDHYFMETISANLPNSPTPLPFSMY-PKSSTANLGMAIICEK 1002

Query: 961  FSKVDSVGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIKTI 1003
                 S GSL L SS DV+ +P VRFNY+SHP DL++CV  VRK+G++L +K+ME+ K +
Sbjct: 1003 LRHPLSSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSMERFKMV 1062

BLAST of Sed0018061 vs. NCBI nr
Match: KAF4368106.1 (hypothetical protein G4B88_001010 [Cannabis sativa])

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 558/1050 (53.14%), Postives = 723/1050 (68.86%), Query Frame = 0

Query: 1    MKFVHDANEFP-AKEVYDYIVIGGGTAGCPLATTLSSKFSVLILETGSDPNKYPSVLSEQ 60
            MK VHDA E    +E YDYI+IGGGTAGCPLA TLS  +SVL+LE GS P   P+VL   
Sbjct: 43   MKSVHDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTANPNVLHLH 102

Query: 61   GLLNAFAAKDD--GINPFNRFISEDGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVN 120
            G       +++   + P  RF SEDGVEN RGRVLGG+SM+NAGF+SRG   FF   GV 
Sbjct: 103  GFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGFFSKPGVK 162

Query: 121  WDMEMVEEAYEWVEESVVSRPSLNAWQSAFRSSLLESGVVPDNGFSLKHIVGTKTSGSIF 180
            W+M+ VE+AYEWVEES+V RP L  WQS+FR +LLE GV PDN F L H +GTK SGS F
Sbjct: 163  WEMDRVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGTKISGSTF 222

Query: 181  DGKGNRHGAVELLNKAEPRNLKIAIRATVQRIIFSGL--------------------SAI 240
            D  G RHGAVELLNK    NLK+ + ATV++I FS                       AI
Sbjct: 223  DEVGRRHGAVELLNKGNLNNLKVVVHATVEKIFFSTKVSRNYHSGDDNDHDNNPPKPYAI 282

Query: 241  GVLYSDSKGKLHKAFIRNKGEIIVSAGALGSPQLLLLSGIGPKSHLSSLKLPVVLHQRHV 300
            GV+YSDSKGK H   +++KGE+I+SAGA+GSPQLLLLSGIGP S+LSSL +P+V  Q +V
Sbjct: 283  GVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVHSQPNV 342

Query: 301  GQFMSDNPRFTSSIVLPFPVLSQTSAKVVGILENNIYLQSFASSLPFSFPPSFSLLPPKS 360
            G F++DNPR   ++++P P    +  +VVGI  N+ ++++ +++LP S  P    + PKS
Sbjct: 343  GDFIADNPRNNINLIIPSPT-DPSPVQVVGI-TNHYFIETISANLPASLTPLPFSVYPKS 402

Query: 361  NSVNMTLAIVAGKFSTVDSVGSLRLTSSVNAKKNPIVRFNYYSHPDDVSRCVRGVRKIGD 420
            ++ ++ + ++  K     S GSL L S  + +  P VRFNY+SHP D+S+CV GVRKIG+
Sbjct: 403  STAHLGMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVNGVRKIGE 462

Query: 421  LLRTETMERIKTRDLEGKKGFRFLGPALPSNLSDYSSVREFCRETVTSYWHYHGGCLVGK 480
            +L T  MER K  D   ++ F + GP+LP+N SD S++ EFCR +VT+ WHYHGGC VGK
Sbjct: 463  MLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGK 522

Query: 481  VVDGDYKVMGIKNLRVVDGSTFSESPGTNPMATVMMLGR-----------------YMKF 540
            VVDGD++VMG+ +LRVVDGSTF  SPGTNP ATVMMLGR                 YMK 
Sbjct: 523  VVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQEREAEANVEYMKS 582

Query: 541  VHDANEFP-EKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQG-L 600
            V+DA E   E+E YDYI+IG   AGCPLAAT+S  +SVL+LERGS P   P+VL+  G L
Sbjct: 583  VYDATEMSLEEEYYDYIIIG---AGCPLAATLSENYSVLVLERGSVPTSNPNVLHLSGFL 642

Query: 601  FNAFATKDDGE-NPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWDM 660
             N    +++    P QRF SE+GVEN+RGRVLGGSSM+NAGF+SRG   F+  +GV+W+M
Sbjct: 643  ANLMQEEEETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSRGDEGFYSKSGVKWEM 702

Query: 661  EMVEKAYEWVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDGE 720
            + VEKAYEWVEE++V  P L  W S+FR ALL+ G+ P+NGF L H +GTK  GS FD  
Sbjct: 703  DRVEKAYEWVEESIVFRPKLPVWQSSFRDALLEVGVGPNNGFDLNHKLGTKISGSTFDEV 762

Query: 721  GNRHGAVELLNKAKPTNLKIAIRATVERIIFSDLSASGVLYSD-----SKGRLHKALIRN 780
            G RHGAVELLNK    NLK+ + ATV+RIIFS   ++     D     S  + H  L+R+
Sbjct: 763  GRRHGAVELLNKGNLNNLKVGVHATVDRIIFSTKVSNNYHSGDDSDQNSPRKSHTVLVRH 822

Query: 781  KGEIMVSAGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSANIVLPF 840
            KGEI++SAGA+GSPQLLLLSG+GP S+LSSL +P+VL Q  VG F++DNPR + N+++PF
Sbjct: 823  KGEIILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIADNPRNNINLIIPF 882

Query: 841  PLLPTSGKVVGILDDNIYFQSFAGSLPFS-LPSSFSLLPPRSDFVDMSLAIFFGKFSKVD 900
            P  P++ +VVGI  D+ + ++ + +LP S  P  FS+  P+S   ++ +AI   K     
Sbjct: 883  PTEPSAVQVVGI-TDHYFMETISANLPNSPTPLPFSMY-PKSSTANLGMAIICEKLRHPL 942

Query: 901  SVGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIKTIDLEGK 960
            S GSL L SS DV+ +P VRFNY+SHP DL++CV  VRK+G++L +K+ME+ K +D   +
Sbjct: 943  SSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSMERFKMVDSNEE 1002

Query: 961  KGFRFLGSPLPENLSDYSSVGQFCREILATYWHYHGGCLVGKVVDGDYKVMGIKNLRVVD 1002
            + F + G  LP N SD S++ +FCR  + T WHYHGGC VGKVVDGD++V G+ +LRVVD
Sbjct: 1003 RNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGKVVDGDFRVTGVNSLRVVD 1062

BLAST of Sed0018061 vs. NCBI nr
Match: KAF4383869.1 (hypothetical protein G4B88_016302, partial [Cannabis sativa])

HSP 1 Score: 1045.8 bits (2703), Expect = 2.4e-301
Identity = 544/1048 (51.91%), Postives = 708/1048 (67.56%), Query Frame = 0

Query: 1    MKFVHDANEFP-AKEVYDYIVIGGGTAGCPLATTLSSKFSVLILETGSDPNKYPSVLSEQ 60
            MK V+DA E    +E YDYI+IGGGTAGCPLA TLS  +SVL+LE GS P   P+VL   
Sbjct: 100  MKSVYDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTANPNVLHLH 159

Query: 61   GLLNAFAAKDD--GINPFNRFISEDGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVN 120
            G       +++   + P  RF SEDGVEN RGRVLGG+SM+NAGF+SRG   FF   GV 
Sbjct: 160  GFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGFFSKPGVK 219

Query: 121  WDMEMVEEAYEWVEESVVSRPSLNAWQSAFRSSLLESGVVPDNGFSLKHIVGTKTSGSIF 180
            W+M+ VE+AYEWVEES+V RP L  WQS+FR +LLE GV PDN F L H +GTK SGS F
Sbjct: 220  WEMDGVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGTKISGSTF 279

Query: 181  DGKGNRHGAVELLNKAEPRNLKIAIRATVQRIIFSGL--------------------SAI 240
            D  G RHGAVELLNK    NLK+ + ATV++IIFS                       AI
Sbjct: 280  DEVGRRHGAVELLNKGNLNNLKVVVHATVEKIIFSTKVSRNYHSGDDNDHDNNPPKPYAI 339

Query: 241  GVLYSDSKGKLHKAFIRNKGEIIVSAGALGSPQLLLLSGIGPKSHLSSLKLPVVLHQRHV 300
            GV+YSDSKGK H   +++KGE+I+SAGA+GSPQLLLLSGIGP S+LSSL +P+V  Q +V
Sbjct: 340  GVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVHSQPNV 399

Query: 301  GQFMSDNPRFTSSIVLPFPVLSQTSAKVVGILENNIYLQSFASSLPFSFPPSFSLLPPKS 360
            G F++DNPR   ++++P P    +  +VVGI  N+ ++++ +++LP S  P    + PKS
Sbjct: 400  GDFIADNPRNNINLIIPSPT-DPSPVQVVGI-TNHYFIETISANLPASLTPLPFSVYPKS 459

Query: 361  NSVNMTLAIVAGKFSTVDSVGSLRLTSSVNAKKNPIVRFNYYSHPDDVSRCVRGVRKIGD 420
            ++ ++ + ++  K     S GSL L S  + +  P VRFNY+SHP D+S+CV GVRKIG+
Sbjct: 460  STAHLGMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVNGVRKIGE 519

Query: 421  LLRTETMERIKTRDLEGKKGFRFLGPALPSNLSDYSSVREFCRETVTSYWHYHGGCLVGK 480
            +L T  MER K  D   ++ F + GP+LP+N SD S++ EFCR +VT+ WHYHGGC VGK
Sbjct: 520  MLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGK 579

Query: 481  VVDGDYKVMGIKNLRVVDGSTFSESPGTNPMATVMMLG---------------------- 540
            VVDGD++VMG+ +LRVVDGSTF  SPGTNP ATVMMLG                      
Sbjct: 580  VVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQEREAEANVLALAT 639

Query: 541  -------RYMKFVHDANEFP-EKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPN 600
                   RYMK V+DA E   E+E YDYI+IGGGTAGCPLAAT+S  +SVL+LERGS P 
Sbjct: 640  SGSDHDFRYMKSVYDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPT 699

Query: 601  KYPSVLNEQG-LFNAFATKDDGE-NPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHR 660
              P+VL+  G L N    +++    P QRF SE+GVEN+RGRVLGGSSM+NAGF+SRG  
Sbjct: 700  SNPNVLHLSGFLANLMQEEEETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSRGDE 759

Query: 661  EFFETAGVEWDMEMVEKAYEWVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLV 720
             F+  +GV+W+M+ VEKAYEWVEE++V  P L  W S+FR ALL+ G+VP+NGF L H V
Sbjct: 760  GFYSKSGVKWEMDRVEKAYEWVEESIVFRPKLPVWQSSFRDALLEVGVVPNNGFDLNHKV 819

Query: 721  GTKTGGSIFDGEGNRHGAVELLNKAKPTNLKIAIRATVERIIFSDLSASGVLYSDSKGRL 780
            GTK  GS FD  G RHG     + +   + K                A GV+YSDSKG+ 
Sbjct: 820  GTKISGSTFDEVGRRHGNYHSGDDSDQNSPK--------------PYAIGVIYSDSKGKS 879

Query: 781  HKALIRNKGEIMVSAGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFS 840
            H  L+++KGE+++SAGA+GSPQLLLLSG+GP S+LSSL +P+VL Q  VG F++DNPR +
Sbjct: 880  HTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIADNPRNN 939

Query: 841  ANIVLPFPLLPTSGKVVGILDDNIYFQSFAGSLPFS-LPSSFSLLPPRSDFVDMSLAIFF 900
             N+++PFP  P++ +VVGI  D+ + ++ + +LP S  P  FS+  P+S   ++ +AI  
Sbjct: 940  INLIIPFPTEPSAVQVVGI-TDHYFMETISANLPNSPTPLPFSMY-PKSSTANLGMAIIC 999

Query: 901  GKFSKVDSVGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIK 960
             K     S GSL L SS DV+ +P VRFNY+SHP DL++CV  VRK+G++L +K+ME+ K
Sbjct: 1000 EKLRHPLSSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSMERFK 1059

Query: 961  TIDLEGKKGFRFLGSPLPENLSDYSSVGQFCREILATYWHYHGGCLVGKVVDGDYKVMGI 993
             +D   ++ F + G  LP N SD S++ +FCR  + T WHYHGGC VGKVVDGD++V G+
Sbjct: 1060 MVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGKVVDGDFRVTGV 1119

BLAST of Sed0018061 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 1.2e-154
Identity = 265/510 (51.96%), Postives = 365/510 (71.57%), Query Frame = 0

Query: 497  YMKFVHDANEFPEKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQ 556
            Y++F +DA +   +  YDY+++GGGT+GCPLAAT+S K+ VL+LERGS P  YP+VL   
Sbjct: 38   YLRFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTAD 97

Query: 557  GLFNAFATKDDGENPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWD 616
            G       +DDG+ P +RFVSE+G++N+RGRVLGG+SM+NAG Y+R +   +  +GV+WD
Sbjct: 98   GFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYARANTSIYSASGVDWD 157

Query: 617  MEMVEKAYEWVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDG 676
            M++V K YEWVE+T+V +P+   W S   +A L++G+ P++GFSL H  GT+  GS FD 
Sbjct: 158  MDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDN 217

Query: 677  EGNRHGAVELLNKAKPTNLKIAIRATVERIIFSD---LSASGVLYSDSKGRLHKALIRNK 736
            +G RH A ELLNK    NL++ + A+VE+IIFS+   L+A+GV+Y DS G  H+A +R+K
Sbjct: 218  KGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHRAFVRSK 277

Query: 737  GEIMVSAGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSANIVLPFP 796
            GE++VSAG +G+PQLLLLSGVGP+S+LSSL +PVVL   YVGQFL DNPR   NI+ P P
Sbjct: 278  GEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNP 337

Query: 797  LLPTSGKVVGILDDNIYFQSFAGSLPFSLPSSFSLLPPRS-DFVDMSLAIFFGKFSKVDS 856
            + PT   V+GI +D  ++Q    SLPF+ P  FS  P  S    + + A F  K +   S
Sbjct: 338  IEPTIVTVLGISND--FYQCSFSSLPFTTP-PFSFFPSTSYPLPNSTFAHFASKVAGPLS 397

Query: 857  VGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIKTIDLEGKK 916
             GSL L SS++V+ SP V+FNYYS+P DL+ CV G++K+G+LL T  ++  K  DL G +
Sbjct: 398  YGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGIE 457

Query: 917  GFRFLGSPLPENLSDYSSVGQFCREILATYWHYHGGCLVGKVVDGDYKVMGIKNLRVVDG 976
            GF  LG PLP++ +D ++   FCRE +A+YWHYHGGCLVGKV+DGD++V GI  LRVVDG
Sbjct: 458  GFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGIDALRVVDG 517

Query: 977  STFSESPGTNPMATLMMLGRYVGLKVLKER 1003
            STF  +P ++P    +MLGRYVG+K+L+ER
Sbjct: 518  STFPYTPASHPQGFYLMLGRYVGIKILQER 544

BLAST of Sed0018061 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.5e-152
Identity = 261/510 (51.18%), Postives = 364/510 (71.37%), Query Frame = 0

Query: 497  YMKFVHDANEFPEKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQ 556
            Y+ F +DA +   +  YDY+++GGGT+GCPLAAT+S K+ VL+LERGS P  YP+VL   
Sbjct: 38   YLSFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTAD 97

Query: 557  GLFNAFATKDDGENPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWD 616
            G       +DDG+ P +RFVSE+G++N+RGRVLGG+S++NAG Y+R +   +  +GV+WD
Sbjct: 98   GFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSIINAGVYARANTSIYSASGVDWD 157

Query: 617  MEMVEKAYEWVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDG 676
            M++V + YEWVE+T+V +P+  +W S  ++A L++G+ P++GFSL H  GT+  GS FD 
Sbjct: 158  MDLVNQTYEWVEDTIVYKPNSQSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDN 217

Query: 677  EGNRHGAVELLNKAKPTNLKIAIRATVERIIFSD---LSASGVLYSDSKGRLHKALIRNK 736
            +G RH A ELLNK    NL++ + A+VE+IIFS+   L+A+GV+Y DS G  H+A +R+K
Sbjct: 218  KGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSK 277

Query: 737  GEIMVSAGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSANIVLPFP 796
            GE++VSAG +G+PQLLLLSGVGP+S+LSSL +PVVL   YVGQFL DNPR   NI+ P P
Sbjct: 278  GEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNP 337

Query: 797  LLPTSGKVVGILDDNIYFQSFAGSLPFSLPSSFSLLPPRS-DFVDMSLAIFFGKFSKVDS 856
            + PT   V+GI +D  ++Q    SLPF+ P  F   P  S    + + A F  K +   S
Sbjct: 338  IEPTIVTVLGISND--FYQCSFSSLPFTTP-PFGFFPSASYPLPNSTFAHFASKVAGPLS 397

Query: 857  VGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIKTIDLEGKK 916
             GSL L SS++V+ SP V+FNYYS+  DL+ CV G++K+G+LL T  ++  K  DL G +
Sbjct: 398  YGSLTLKSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVE 457

Query: 917  GFRFLGSPLPENLSDYSSVGQFCREILATYWHYHGGCLVGKVVDGDYKVMGIKNLRVVDG 976
            GF  LG PLP++ +D ++   FCRE +A+YWHYHGGCLVGKV+DGD++V GI  LRVVDG
Sbjct: 458  GFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGINALRVVDG 517

Query: 977  STFSESPGTNPMATLMMLGRYVGLKVLKER 1003
            STF  +P ++P    +MLGRYVG+K+L+ER
Sbjct: 518  STFPYTPASHPQGFYLMLGRYVGIKILQER 544

BLAST of Sed0018061 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.0e-149
Identity = 257/511 (50.29%), Postives = 356/511 (69.67%), Query Frame = 0

Query: 497  YMKFVHDANEFPEKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQ 556
            Y+ FV+DA +   +  YDYI++GGGTAGCPLAAT+S+ +SVL+LERGS P +YP++L   
Sbjct: 38   YLSFVYDATDPELEGSYDYIIVGGGTAGCPLAATLSANYSVLVLERGSLPTEYPNLLISD 97

Query: 557  GLFNAFATKDDGENPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWD 616
            G       +DDG+ P +RFVSE+G++N+RGRVLGG+SM+NAG Y R +  FF   G+EWD
Sbjct: 98   GFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWD 157

Query: 617  MEMVEKAYEWVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDG 676
            M++V + YEWVE+T+V EP    W +   +A L++GI+P+NGFS+ HL GT+  GS FD 
Sbjct: 158  MDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDN 217

Query: 677  EGNRHGAVELLNKAKPTNLKIAIRATVERIIFSD----LSASGVLYSDSKGRLHKALIRN 736
             G RH + ELLNK  P NL++A++A VE+IIFS     ++A GV+Y+DS G  H+A +R 
Sbjct: 218  NGTRHASDELLNKGDPNNLRVAVQAAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRG 277

Query: 737  KGEIMVSAGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSANIVLPF 796
            +GE+++SAG +GSPQLLLLSGVGP+S+L+SL + VV    YVGQ++ DNPR   NI+ P 
Sbjct: 278  EGEVILSAGPIGSPQLLLLSGVGPESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPN 337

Query: 797  PLLPTSGKVVGILDDNIYFQSFAGSLPFSLPSSFSLLPPRS-DFVDMSLAIFFGKFSKVD 856
            P+  ++  V+GI  D  ++Q    SLPF  P  FS  P  S    + + A    K     
Sbjct: 338  PIEASTVTVLGITSD--FYQCSISSLPFDTP-PFSFFPTTSYPLPNQTFAHIVNKVPGPL 397

Query: 857  SVGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIKTIDLEGK 916
            S G++ LNSS+DV+  P V+FNYYS+  DL+ CV G++K+G++L T  +E  K  DL G 
Sbjct: 398  SHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGI 457

Query: 917  KGFRFLGSPLPENLSDYSSVGQFCREILATYWHYHGGCLVGKVVDGDYKVMGIKNLRVVD 976
             GF  LG PLPEN +D ++   FCRE +A+YWHYHGGCLVGKV+D  ++V GI  LRVVD
Sbjct: 458  DGFNILGIPLPENQTDDAAFETFCRESVASYWHYHGGCLVGKVLDDGFRVTGINALRVVD 517

Query: 977  GSTFSESPGTNPMATLMMLGRYVGLKVLKER 1003
            GSTF  +P ++P    +MLGRY+G+++L+ER
Sbjct: 518  GSTFPSTPASHPQGFYLMLGRYMGIQILQER 545

BLAST of Sed0018061 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 4.9e-148
Identity = 252/514 (49.03%), Postives = 357/514 (69.46%), Query Frame = 0

Query: 497  YMKFVHDANEFPEKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQ 556
            +M+F+ +A +F  ++ YDYI++GGGTAGCPLAAT+S  F VLLLERG  P   P+V++  
Sbjct: 38   FMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHD 97

Query: 557  GLFNAFATKDDGENPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWD 616
            G        ++ ++P Q F+SEEGV N RGRVLGGSS +NAGFYSR  ++FFE +G+ WD
Sbjct: 98   GFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWD 157

Query: 617  MEMVEKAYEWVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDG 676
            +  V ++YEWVE  +V  P L  W +A R ALL+ G+ P NGF+L H VGTK GGS FD 
Sbjct: 158  LSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDR 217

Query: 677  EGNRHGAVELLNKAKPTNLKIAIRATVERIIF--------SDLSASGVLYSDSKGRLHKA 736
             G RH + +LL  A+ +N+++A+ ATVER++         S++SA GV+Y D  GR H A
Sbjct: 218  TGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHA 277

Query: 737  LIRNKGEIMVSAGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSANI 796
            LIR++GE+++SAGA+GSPQLL LSG+GP+S+LS+  +PV LDQ +VG F+ DNPR   +I
Sbjct: 278  LIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISI 337

Query: 797  VLPFPLLPTSGKVVGILDDNIYFQSFAGSLPFSLPSSFSLLPPRSDFVDMSLAIFFGKFS 856
            V P P+  +  +VVG+ +D  + ++ +  +PF+ P     +   +  + + +     K  
Sbjct: 338  VPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKIL 397

Query: 857  KVDSVGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIKTIDL 916
               S+G LRL +STDV+ +P+VRFNY+S P DL RCV G RK+G++L+++ M+     + 
Sbjct: 398  GPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREW 457

Query: 917  EGKKGFRFLGSPLPENLSDYSSVGQFCREILATYWHYHGGCLVGKVVDGDYKVMGIKNLR 976
             G + FRF+G+PLP + S+   +  FCR  ++T WHYHGG +VGKVVD D KV+G+ +LR
Sbjct: 458  FGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLR 517

Query: 977  VVDGSTFSESPGTNPMATLMMLGRYVGLKVLKER 1003
            +VDGSTF+ SPGTNP ATLMMLGRY+GLK+L+ER
Sbjct: 518  LVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550

BLAST of Sed0018061 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 7.0e-147
Identity = 260/511 (50.88%), Postives = 353/511 (69.08%), Query Frame = 0

Query: 497  YMKFVHDANEFPEKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQ 556
            Y+KFV++A +   +  YDYIVIGGGT+GCPLAAT+S K+ VLLLERG+   +YP+ L   
Sbjct: 38   YLKFVYNATDTSLEGSYDYIVIGGGTSGCPLAATLSEKYKVLLLERGTIATEYPNTLTAD 97

Query: 557  GLFNAFATKDDGENPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWD 616
            G       +DDG+ P +RFVSE+G++N+R R+LGG++++NAG Y+R +  F+   G+EWD
Sbjct: 98   GFAYNLQQQDDGKTPVERFVSEDGIDNVRARILGGTTIINAGVYARANISFYSQTGIEWD 157

Query: 617  MEMVEKAYEWVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDG 676
            +++V K YEWVE+ +V +P+  +W S      L++GI+PDNGFSL H  GT+  GS FD 
Sbjct: 158  LDLVNKTYEWVEDAIVVKPNNQSWQSVIGEGFLEAGILPDNGFSLDHEAGTRLTGSTFDN 217

Query: 677  EGNRHGAVELLNKAKPTNLKIAIRATVERIIF----SDLSASGVLYSDSKGRLHKALIRN 736
             G RH A ELLNK  P NL +A++A+VE+I+F    S+LSA GV+Y+DS G  H+A +R 
Sbjct: 218  NGTRHAADELLNKGDPNNLLVAVQASVEKILFSSNTSNLSAIGVIYTDSDGNSHQAFVRG 277

Query: 737  KGEIMVSAGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSANIVLPF 796
             GE++VSAG +G+PQLLLLSGVGP+S+LSSL + VV    YVGQFL +NPR   N   P 
Sbjct: 278  NGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNITVVQPNPYVGQFLYNNPRNFINNFPPN 337

Query: 797  PLLPTSGKVVGILDDNIYFQSFAGSLPFSLPSSFSLLPPRS-DFVDMSLAIFFGKFSKVD 856
            P+  +   V+GI  D  Y+Q    SLPFS P  FSL P  S    + + A    +     
Sbjct: 338  PIEASVVTVLGIRSD--YYQVSLSSLPFSTP-PFSLFPTTSYPLPNSTFAHIVSQVPGPL 397

Query: 857  SVGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIKTIDLEGK 916
            S GS+ LNSS+DV+ +P ++FNYYS+  DLA CV G++K+GDLL+TK +E  K  D+ G 
Sbjct: 398  SHGSVTLNSSSDVRIAPNIKFNYYSNSTDLANCVSGMKKLGDLLRTKALEPYKARDVLGI 457

Query: 917  KGFRFLGSPLPENLSDYSSVGQFCREILATYWHYHGGCLVGKVVDGDYKVMGIKNLRVVD 976
             GF +LG PLPEN +D +S   FC + +A+YWHYHGG LVGKV+D  ++VMGIK LRVVD
Sbjct: 458  DGFNYLGVPLPENQTDDASFETFCLDNVASYWHYHGGSLVGKVLDDSFRVMGIKALRVVD 517

Query: 977  GSTFSESPGTNPMATLMMLGRYVGLKVLKER 1003
             STF   P ++P    +MLGRYVGL++L+ER
Sbjct: 518  ASTFPYEPNSHPQGFYLMLGRYVGLQILQER 545

BLAST of Sed0018061 vs. ExPASy TrEMBL
Match: A0A7J6GLR3 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_016288 PE=3 SV=1)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 563/1037 (54.29%), Postives = 735/1037 (70.88%), Query Frame = 0

Query: 1    MKFVHDANEFPAKEVYDYIVIGGGTAGCPLATTLSSKFSVLILETGSDPNKYPSVLSEQG 60
            MK V++A + P +E YDYIV+GGGTAGCPLA TLS K+SVL+LE G+ P  +P+ L   G
Sbjct: 35   MKSVYNATDMPLEEEYDYIVVGGGTAGCPLAATLSEKYSVLVLERGNVPKAHPNTLVASG 94

Query: 61   LLNAF--AAKDDGINPFNRFISEDGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVNW 120
             L     A  DDG  P  RF SE+GVE++RGRVLGG+SM+NA F+S    +F   +GV W
Sbjct: 95   FLTNLVEADDDDGDTPAQRFTSEEGVESVRGRVLGGTSMINAAFFSEADNDFLAKSGVEW 154

Query: 121  DMEMVEEAYEWVEESVVSRPSLNAWQSAFRSSLLESGVVPDNGFSLKHIVGTKTSGSIFD 180
            D + VE+AYEWV+  +VS  +L+ WQSA + +LLE+GV PDNG + KH VGTK SGS FD
Sbjct: 155  DNDEVEKAYEWVKSKIVSYSNLSTWQSAVKEALLEAGVGPDNGVTTKHKVGTKQSGSTFD 214

Query: 181  GKGNRHGAVELLNKAEPRNLKIAIRATVQRIIF----SGLSAIGVLYSDSKGKLHKAFIR 240
              G RHGAVELLNK   +N+KIAI A V++IIF    S  SAIGV+Y+DSKGK HKA IR
Sbjct: 215  NMGRRHGAVELLNKGNLKNMKIAIHAYVKKIIFSTKSSNPSAIGVIYTDSKGKYHKALIR 274

Query: 241  NKGEIIVSAGALGSPQLLLLSGIGPKSHLSSLKLPVVLHQRHVGQFMSDNPRFTSSIVLP 300
            NKGE+I+SAGALGSPQLLLLSGIGPKS+LSS  +P+V  Q +VG+FM+DNPR   ++++P
Sbjct: 275  NKGEVILSAGALGSPQLLLLSGIGPKSYLSSHHIPIVHSQSNVGKFMADNPRNNINLIIP 334

Query: 301  FPVLSQTSAKVVGI-----LENNIYLQSFA-SSLPFSFPPSFSLLPPKSNSVNMTLAIVA 360
            FP    + A+VVGI     +E   Y   FA ++LPFSF P+  L PP+      +LA + 
Sbjct: 335  FP-FEGSGAQVVGITDDYFIETVSYSLPFAPTTLPFSFYPN-PLTPPQ-----FSLASIV 394

Query: 361  GKFSTVDSVGSLRLTSSVNAKKNPIVRFNYYSHPDDVSRCVRGVRKIGDLLRTETMERIK 420
             K     S GSL L S+ + K  P VRFNY+S+P D++RCV  +RK+G++L T++++R K
Sbjct: 395  EKIVGPLSTGSLTLASTKDVKITPHVRFNYFSNPIDLARCVSAMRKVGEMLETKSLDRFK 454

Query: 421  TRDLEGKKGFRFLGPALPSNLSDYSSVREFCRETVTSYWHYHGGCLVGKVVDGDYKVMGI 480
             +DL G + F +LGP LP+N SD S++ ++CR +VT++WHYHGGCLVGKVVDGD+KV+G 
Sbjct: 455  YKDLNGARDFMYLGPPLPANQSDNSAMEDYCRSSVTTFWHYHGGCLVGKVVDGDFKVIGT 514

Query: 481  KNLRVVDGSTFSESPGTNPMATVMMLGR----------------------YMKFVHDANE 540
             +LRVVDGSTF  SPGTNP AT+MM+GR                      YMK V++A +
Sbjct: 515  NSLRVVDGSTFVISPGTNPQATLMMIGRVGDNNGPFGLGIAVSGADYDFSYMKSVYNATD 574

Query: 541  FPEKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQG-LFNAFATK 600
             P +EEYDYIV+GGGTAGCPLAAT+S K+SVL+LERG+ P  +P+ L   G L N     
Sbjct: 575  MPLEEEYDYIVVGGGTAGCPLAATLSEKYSVLVLERGNVPKAHPNTLVASGFLTNLIEAD 634

Query: 601  DDGENPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWDMEMVEKAYE 660
            DDG+ P QRF S EGVEN+RGRVLGG+SM+NA F+S    +F   +GV+WD + VEKAYE
Sbjct: 635  DDGDTPAQRFTS-EGVENVRGRVLGGTSMINAAFFSEADNDFMVKSGVKWDNDEVEKAYE 694

Query: 661  WVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDGEGNRHGAVE 720
            WV E++VS  +++ W  A + ALL++G+ PDNG + +H +GTK  GS FD  G RHGAVE
Sbjct: 695  WVRESIVSFSNMSTWQFAVKEALLEAGVGPDNGVTTKHKIGTKQSGSTFDNMGRRHGAVE 754

Query: 721  LLNKAKPTNLKIAIRATVERIIFSDLSAS------GVLYSDSKGRLHKALIRNKGEIMVS 780
            LLNK    N+KIAI A V ++IFS  S+S      GV+Y+DSKG+ HKALIRNKGE+++S
Sbjct: 755  LLNKGDLQNMKIAIHAYVNKVIFSTKSSSSNPSAIGVIYTDSKGKSHKALIRNKGEVILS 814

Query: 781  AGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSANIVLPFPLLPTSG 840
            AGA+GSPQLLLLSG+GPKS+LSS  +P++  Q  VGQF++DNPR + N+++PFPL  ++ 
Sbjct: 815  AGALGSPQLLLLSGIGPKSYLSSQHIPIIHSQSNVGQFMADNPRNNINLIIPFPLEGSAV 874

Query: 841  KVVGILDDNIYFQSFAGSLPFSLPS-SFSLLPPRSDFVDMSLAIFFGKFSKVDSVGSLRL 900
            +VVGI +D  + ++ + SLPF+  +  FS  P        SLA    K     S GSL L
Sbjct: 875  QVVGITND-YFIETVSYSLPFAPTTLPFSFYPNPLTQPQFSLATIAAKIVGPLSTGSLTL 934

Query: 901  NSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIKTIDLEGKKGFRFLG 960
             S+ DVK +P VRFNY+S+P DLARCV  +RK+G++L+TK++++ K  DL G + F FLG
Sbjct: 935  ASTKDVKITPHVRFNYFSNPIDLARCVSAMRKLGEMLETKSLDRFKYKDLNGARDFMFLG 994

Query: 961  SPLPENL-SDYSSVGQFCREILATYWHYHGGCLVGKVVDGDYKVMGIKNLRVVDGSTFSE 995
              LP N  S+ S +  +CR  + T WHYHGGCLVGKVVDGD+KV+G  +LRVVDGSTF  
Sbjct: 995  PTLPSNYQSNNSGMEDYCRSSVTTIWHYHGGCLVGKVVDGDFKVIGTNSLRVVDGSTFVI 1054

BLAST of Sed0018061 vs. ExPASy TrEMBL
Match: A0A7J6DSU4 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=F8388_026317 PE=3 SV=1)

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 569/1116 (50.99%), Postives = 733/1116 (65.68%), Query Frame = 0

Query: 1    MKFVHDANEFP-AKEVYDYIVIGGGTAGCPLATTLSSKFSVLILETGSDPNKYPSVLSEQ 60
            MK VHDA E    +E YDYI+IGGGTAGCPLA TLS  +SVL+LE GS P   P+VL   
Sbjct: 43   MKSVHDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTANPNVLHLH 102

Query: 61   GLLNAFAAKDD--GINPFNRFISEDGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVN 120
            G       +++   + P  RF SEDGVEN RGRVLGG+SM+NAGF+SRG   FF   GV 
Sbjct: 103  GFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGFFSKPGVK 162

Query: 121  WDMEMVEEAYEWVEESVVSRPSLNAWQSAFRSSLLESGVVPDNGFSLKHIVGTKTSGSIF 180
            W+M+ VE+AYEWVEES+V RP L  WQS+FR +LLE GV PDN F L H +GTK SGS F
Sbjct: 163  WEMDRVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGTKISGSTF 222

Query: 181  DGKGNRHGAVELLNKAEPRNLKIAIRATVQRIIFSGL--------------------SAI 240
            D  G RHGAVELLNK    NLK+ + ATV++I FS                       AI
Sbjct: 223  DEVGRRHGAVELLNKGNLNNLKVVVHATVEKIFFSTKVSRNYHSGDDNDHDNNPPKPYAI 282

Query: 241  GVLYSDSKGKLHKAFIRNKGEIIVSAGALGSPQLLLLSGIGPKSHLSSLKLPVVLHQRHV 300
            GV+YSDSKGK H   +++KGE+I+SAGA+GSPQLLLLSGIGP S+LSSL +P+V  Q +V
Sbjct: 283  GVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVHSQPNV 342

Query: 301  GQFMSDNPRFTSSIVLPFPVLSQTSAKVVGILENNIYLQSFASSLPFSFPPSFSLLPPKS 360
            G F++DNPR   ++++P P    +  +VVGI  N+ ++++ +++LP S  P    + PKS
Sbjct: 343  GDFIADNPRNNINLIIPSPT-DPSPVQVVGI-TNHYFIETISANLPASLTPLPFSVYPKS 402

Query: 361  NSVNMTLAIVAGKFSTVDSVGSLRLTSSVNAKKNPIVRFNYYSHPDDVSRCVRGVRKIGD 420
            ++ ++ + ++  K     S GSL L S  + +  P VRFNY+SHP D+S+CV GVRKIG+
Sbjct: 403  STAHLGMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVNGVRKIGE 462

Query: 421  LLRTETMERIKTRDLEGKKGFRFLGPALPSNLSDYSSVREFCRETVTSYWHYHGGCLVGK 480
            +L T  MER K  D   ++ F + GP+LP+N SD S++ EFCR +VT+ WHYHGGC VGK
Sbjct: 463  MLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGK 522

Query: 481  VVDGDYKVMGIKNLRVVDGSTFSESPGTNPMATVMMLG---------------------- 540
            VVDGD++VMG+ +LRVVDGSTF  SPGTNP ATVMMLG                      
Sbjct: 523  VVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQEREAEANVEGDIE 582

Query: 541  ---------------------------------------------RYMKFVHDANEFP-E 600
                                                         RYMK V+DA E   E
Sbjct: 583  RREIDEKDRVKDYLSTVALRVSRWATSDGQQQVLALATSGSDHDFRYMKSVYDATEMSLE 642

Query: 601  KEEYDYIVIGGG--TAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQG-LFNAFATKD 660
            +E YDYI+IGGG  TAGCPLAAT+S  +SVL+LERGS P   P+VL+  G L N    ++
Sbjct: 643  EEYYDYIIIGGGTLTAGCPLAATLSENYSVLVLERGSVPTSNPNVLHLSGFLANLMQEEE 702

Query: 661  DGE-NPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWDMEMVEKAYE 720
            +    P QRF SE+GVEN+RGRVLGGSSM+NAGF+SRG   F+  +GV+W+M+ VEKAYE
Sbjct: 703  ETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSRGDEGFYSKSGVKWEMDRVEKAYE 762

Query: 721  WVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDGEGNRHGAVE 780
            WVEE++V  P L  W S+FR ALL+ G+ P+NGF L H +GTK  GS FD  G RHGAVE
Sbjct: 763  WVEESIVFRPKLPVWQSSFRDALLEVGVGPNNGFDLNHKLGTKISGSTFDEVGRRHGAVE 822

Query: 781  LLNKAKPTNLKIAIRATVERIIFSDL------------------SASGVLYSDSKGRLHK 840
            LLNK    NLK+ + ATV+RIIFS                     A GV+YSDSKG+ H 
Sbjct: 823  LLNKGNLNNLKVGVHATVDRIIFSTKVSNNYHSGDDSDQNSPKPYAIGVMYSDSKGKSHT 882

Query: 841  ALIRNKGEIMVSAGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSAN 900
             L+R+KGEI++SAGA+GSPQLLLLSG+GP S+LSSL +P+VL Q  VG F++DNPR + N
Sbjct: 883  VLVRHKGEIILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIADNPRNNIN 942

Query: 901  IVLPFPLLPTSGKVVGILDDNIYFQSFAGSLPFS-LPSSFSLLPPRSDFVDMSLAIFFGK 960
            +++PFP  P++ +VVGI  D+ + ++ + +LP S  P  FS+  P+S   ++ +AI   K
Sbjct: 943  LIIPFPTEPSAVQVVGI-TDHYFMETISANLPNSPTPLPFSMY-PKSSTANLGMAIICEK 1002

Query: 961  FSKVDSVGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIKTI 1003
                 S GSL L SS DV+ +P VRFNY+SHP DL++CV  VRK+G++L +K+ME+ K +
Sbjct: 1003 LRHPLSSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSMERFKMV 1062

BLAST of Sed0018061 vs. ExPASy TrEMBL
Match: A0A7J6FBM4 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_001010 PE=3 SV=1)

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 558/1050 (53.14%), Postives = 723/1050 (68.86%), Query Frame = 0

Query: 1    MKFVHDANEFP-AKEVYDYIVIGGGTAGCPLATTLSSKFSVLILETGSDPNKYPSVLSEQ 60
            MK VHDA E    +E YDYI+IGGGTAGCPLA TLS  +SVL+LE GS P   P+VL   
Sbjct: 43   MKSVHDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTANPNVLHLH 102

Query: 61   GLLNAFAAKDD--GINPFNRFISEDGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVN 120
            G       +++   + P  RF SEDGVEN RGRVLGG+SM+NAGF+SRG   FF   GV 
Sbjct: 103  GFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGFFSKPGVK 162

Query: 121  WDMEMVEEAYEWVEESVVSRPSLNAWQSAFRSSLLESGVVPDNGFSLKHIVGTKTSGSIF 180
            W+M+ VE+AYEWVEES+V RP L  WQS+FR +LLE GV PDN F L H +GTK SGS F
Sbjct: 163  WEMDRVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGTKISGSTF 222

Query: 181  DGKGNRHGAVELLNKAEPRNLKIAIRATVQRIIFSGL--------------------SAI 240
            D  G RHGAVELLNK    NLK+ + ATV++I FS                       AI
Sbjct: 223  DEVGRRHGAVELLNKGNLNNLKVVVHATVEKIFFSTKVSRNYHSGDDNDHDNNPPKPYAI 282

Query: 241  GVLYSDSKGKLHKAFIRNKGEIIVSAGALGSPQLLLLSGIGPKSHLSSLKLPVVLHQRHV 300
            GV+YSDSKGK H   +++KGE+I+SAGA+GSPQLLLLSGIGP S+LSSL +P+V  Q +V
Sbjct: 283  GVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVHSQPNV 342

Query: 301  GQFMSDNPRFTSSIVLPFPVLSQTSAKVVGILENNIYLQSFASSLPFSFPPSFSLLPPKS 360
            G F++DNPR   ++++P P    +  +VVGI  N+ ++++ +++LP S  P    + PKS
Sbjct: 343  GDFIADNPRNNINLIIPSPT-DPSPVQVVGI-TNHYFIETISANLPASLTPLPFSVYPKS 402

Query: 361  NSVNMTLAIVAGKFSTVDSVGSLRLTSSVNAKKNPIVRFNYYSHPDDVSRCVRGVRKIGD 420
            ++ ++ + ++  K     S GSL L S  + +  P VRFNY+SHP D+S+CV GVRKIG+
Sbjct: 403  STAHLGMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVNGVRKIGE 462

Query: 421  LLRTETMERIKTRDLEGKKGFRFLGPALPSNLSDYSSVREFCRETVTSYWHYHGGCLVGK 480
            +L T  MER K  D   ++ F + GP+LP+N SD S++ EFCR +VT+ WHYHGGC VGK
Sbjct: 463  MLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGK 522

Query: 481  VVDGDYKVMGIKNLRVVDGSTFSESPGTNPMATVMMLGR-----------------YMKF 540
            VVDGD++VMG+ +LRVVDGSTF  SPGTNP ATVMMLGR                 YMK 
Sbjct: 523  VVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQEREAEANVEYMKS 582

Query: 541  VHDANEFP-EKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQG-L 600
            V+DA E   E+E YDYI+IG   AGCPLAAT+S  +SVL+LERGS P   P+VL+  G L
Sbjct: 583  VYDATEMSLEEEYYDYIIIG---AGCPLAATLSENYSVLVLERGSVPTSNPNVLHLSGFL 642

Query: 601  FNAFATKDDGE-NPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWDM 660
             N    +++    P QRF SE+GVEN+RGRVLGGSSM+NAGF+SRG   F+  +GV+W+M
Sbjct: 643  ANLMQEEEETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSRGDEGFYSKSGVKWEM 702

Query: 661  EMVEKAYEWVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDGE 720
            + VEKAYEWVEE++V  P L  W S+FR ALL+ G+ P+NGF L H +GTK  GS FD  
Sbjct: 703  DRVEKAYEWVEESIVFRPKLPVWQSSFRDALLEVGVGPNNGFDLNHKLGTKISGSTFDEV 762

Query: 721  GNRHGAVELLNKAKPTNLKIAIRATVERIIFSDLSASGVLYSD-----SKGRLHKALIRN 780
            G RHGAVELLNK    NLK+ + ATV+RIIFS   ++     D     S  + H  L+R+
Sbjct: 763  GRRHGAVELLNKGNLNNLKVGVHATVDRIIFSTKVSNNYHSGDDSDQNSPRKSHTVLVRH 822

Query: 781  KGEIMVSAGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSANIVLPF 840
            KGEI++SAGA+GSPQLLLLSG+GP S+LSSL +P+VL Q  VG F++DNPR + N+++PF
Sbjct: 823  KGEIILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIADNPRNNINLIIPF 882

Query: 841  PLLPTSGKVVGILDDNIYFQSFAGSLPFS-LPSSFSLLPPRSDFVDMSLAIFFGKFSKVD 900
            P  P++ +VVGI  D+ + ++ + +LP S  P  FS+  P+S   ++ +AI   K     
Sbjct: 883  PTEPSAVQVVGI-TDHYFMETISANLPNSPTPLPFSMY-PKSSTANLGMAIICEKLRHPL 942

Query: 901  SVGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIKTIDLEGK 960
            S GSL L SS DV+ +P VRFNY+SHP DL++CV  VRK+G++L +K+ME+ K +D   +
Sbjct: 943  SSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSMERFKMVDSNEE 1002

Query: 961  KGFRFLGSPLPENLSDYSSVGQFCREILATYWHYHGGCLVGKVVDGDYKVMGIKNLRVVD 1002
            + F + G  LP N SD S++ +FCR  + T WHYHGGC VGKVVDGD++V G+ +LRVVD
Sbjct: 1003 RNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGKVVDGDFRVTGVNSLRVVD 1062

BLAST of Sed0018061 vs. ExPASy TrEMBL
Match: A0A7J6GP35 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_016302 PE=3 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 1.1e-301
Identity = 544/1048 (51.91%), Postives = 708/1048 (67.56%), Query Frame = 0

Query: 1    MKFVHDANEFP-AKEVYDYIVIGGGTAGCPLATTLSSKFSVLILETGSDPNKYPSVLSEQ 60
            MK V+DA E    +E YDYI+IGGGTAGCPLA TLS  +SVL+LE GS P   P+VL   
Sbjct: 100  MKSVYDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTANPNVLHLH 159

Query: 61   GLLNAFAAKDD--GINPFNRFISEDGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVN 120
            G       +++   + P  RF SEDGVEN RGRVLGG+SM+NAGF+SRG   FF   GV 
Sbjct: 160  GFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGFFSKPGVK 219

Query: 121  WDMEMVEEAYEWVEESVVSRPSLNAWQSAFRSSLLESGVVPDNGFSLKHIVGTKTSGSIF 180
            W+M+ VE+AYEWVEES+V RP L  WQS+FR +LLE GV PDN F L H +GTK SGS F
Sbjct: 220  WEMDGVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGTKISGSTF 279

Query: 181  DGKGNRHGAVELLNKAEPRNLKIAIRATVQRIIFSGL--------------------SAI 240
            D  G RHGAVELLNK    NLK+ + ATV++IIFS                       AI
Sbjct: 280  DEVGRRHGAVELLNKGNLNNLKVVVHATVEKIIFSTKVSRNYHSGDDNDHDNNPPKPYAI 339

Query: 241  GVLYSDSKGKLHKAFIRNKGEIIVSAGALGSPQLLLLSGIGPKSHLSSLKLPVVLHQRHV 300
            GV+YSDSKGK H   +++KGE+I+SAGA+GSPQLLLLSGIGP S+LSSL +P+V  Q +V
Sbjct: 340  GVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVHSQPNV 399

Query: 301  GQFMSDNPRFTSSIVLPFPVLSQTSAKVVGILENNIYLQSFASSLPFSFPPSFSLLPPKS 360
            G F++DNPR   ++++P P    +  +VVGI  N+ ++++ +++LP S  P    + PKS
Sbjct: 400  GDFIADNPRNNINLIIPSPT-DPSPVQVVGI-TNHYFIETISANLPASLTPLPFSVYPKS 459

Query: 361  NSVNMTLAIVAGKFSTVDSVGSLRLTSSVNAKKNPIVRFNYYSHPDDVSRCVRGVRKIGD 420
            ++ ++ + ++  K     S GSL L S  + +  P VRFNY+SHP D+S+CV GVRKIG+
Sbjct: 460  STAHLGMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVNGVRKIGE 519

Query: 421  LLRTETMERIKTRDLEGKKGFRFLGPALPSNLSDYSSVREFCRETVTSYWHYHGGCLVGK 480
            +L T  MER K  D   ++ F + GP+LP+N SD S++ EFCR +VT+ WHYHGGC VGK
Sbjct: 520  MLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGK 579

Query: 481  VVDGDYKVMGIKNLRVVDGSTFSESPGTNPMATVMMLG---------------------- 540
            VVDGD++VMG+ +LRVVDGSTF  SPGTNP ATVMMLG                      
Sbjct: 580  VVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQEREAEANVLALAT 639

Query: 541  -------RYMKFVHDANEFP-EKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPN 600
                   RYMK V+DA E   E+E YDYI+IGGGTAGCPLAAT+S  +SVL+LERGS P 
Sbjct: 640  SGSDHDFRYMKSVYDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPT 699

Query: 601  KYPSVLNEQG-LFNAFATKDDGE-NPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHR 660
              P+VL+  G L N    +++    P QRF SE+GVEN+RGRVLGGSSM+NAGF+SRG  
Sbjct: 700  SNPNVLHLSGFLANLMQEEEETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSRGDE 759

Query: 661  EFFETAGVEWDMEMVEKAYEWVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLV 720
             F+  +GV+W+M+ VEKAYEWVEE++V  P L  W S+FR ALL+ G+VP+NGF L H V
Sbjct: 760  GFYSKSGVKWEMDRVEKAYEWVEESIVFRPKLPVWQSSFRDALLEVGVVPNNGFDLNHKV 819

Query: 721  GTKTGGSIFDGEGNRHGAVELLNKAKPTNLKIAIRATVERIIFSDLSASGVLYSDSKGRL 780
            GTK  GS FD  G RHG     + +   + K                A GV+YSDSKG+ 
Sbjct: 820  GTKISGSTFDEVGRRHGNYHSGDDSDQNSPK--------------PYAIGVIYSDSKGKS 879

Query: 781  HKALIRNKGEIMVSAGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFS 840
            H  L+++KGE+++SAGA+GSPQLLLLSG+GP S+LSSL +P+VL Q  VG F++DNPR +
Sbjct: 880  HTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIADNPRNN 939

Query: 841  ANIVLPFPLLPTSGKVVGILDDNIYFQSFAGSLPFS-LPSSFSLLPPRSDFVDMSLAIFF 900
             N+++PFP  P++ +VVGI  D+ + ++ + +LP S  P  FS+  P+S   ++ +AI  
Sbjct: 940  INLIIPFPTEPSAVQVVGI-TDHYFMETISANLPNSPTPLPFSMY-PKSSTANLGMAIIC 999

Query: 901  GKFSKVDSVGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIK 960
             K     S GSL L SS DV+ +P VRFNY+SHP DL++CV  VRK+G++L +K+ME+ K
Sbjct: 1000 EKLRHPLSSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSMERFK 1059

Query: 961  TIDLEGKKGFRFLGSPLPENLSDYSSVGQFCREILATYWHYHGGCLVGKVVDGDYKVMGI 993
             +D   ++ F + G  LP N SD S++ +FCR  + T WHYHGGC VGKVVDGD++V G+
Sbjct: 1060 MVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGKVVDGDFRVTGV 1119

BLAST of Sed0018061 vs. ExPASy TrEMBL
Match: A0A7J6FBQ5 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_001041 PE=3 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 1.7e-292
Identity = 530/1036 (51.16%), Postives = 704/1036 (67.95%), Query Frame = 0

Query: 1    MKFVHDANEFPAKEVYDYIVIGGGTAGCPLATTLSSKFSVLILETGSDPNKYPSVLSEQG 60
            MK V++A + P  E YDYIVIGGGTAGCPLA TLS K+S+L+LE G+ P  +P+VLS  G
Sbjct: 455  MKSVYNAIDLPLVEEYDYIVIGGGTAGCPLAATLSEKYSILVLERGNTPKAHPNVLSLSG 514

Query: 61   LLNAFAAKDDGINPFNRFISEDGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVNWDM 120
             L  F  +D+G  P  RF SEDGV+NIRGRVLGGSSM+N GF+S    +FF  +GV WDM
Sbjct: 515  FLKNFIEEDNGDTPAQRFTSEDGVDNIRGRVLGGSSMVNGGFFSEADDDFFAKSGVEWDM 574

Query: 121  EMVEEAYEWVEESVVSRPSLNAWQSAFRSSLLESGVVPDNGFSLKHIVGTKTSGSIFDGK 180
              V++AY+WV+  +VS  +L+ WQS  + +LLE G+ PDN  + K+ +GTK SGSIFD  
Sbjct: 575  NEVQKAYKWVKNKIVSYSNLSIWQSVVKQALLEVGIGPDNKVTTKYKIGTKQSGSIFDNM 634

Query: 181  GNRHGAVELLNKAEPRNLKIAIRATVQRIIFSGLSAIGVLYSDSKGKLHKAFIRNKGEII 240
            G RHGAVELLNK   +NL+IAI A V + I           S  K +             
Sbjct: 635  GRRHGAVELLNKGNLKNLRIAIHAYVSQSI-----------STQKRR------------- 694

Query: 241  VSAGALGSPQLLLLSGIGPKSHLSSLKLPVVLHQRHVGQFMSDNPRFTSSIVLPFPVLSQ 300
                ALGSPQLLLLSGIGPKS+LSS  +P+VL Q +VG+FM+DNPR   +++ PFP L  
Sbjct: 695  ---SALGSPQLLLLSGIGPKSYLSSQHIPIVLSQPNVGKFMADNPRNNLNLITPFP-LES 754

Query: 301  TSAKVVGILENNIYLQSFASSLPFS--------FPPSFSL-LPPKSNSVNMTLAIVAGKF 360
            +  +VVGI +   Y+++ + +LPFS        FP ++SL +PP    + +++A + GK 
Sbjct: 755  SILQVVGITK-EYYIETLSYNLPFSPTTLPSSFFPHNYSLAIPP----LQLSVANIVGKV 814

Query: 361  STVDSVGSLRLTSSVNAKKNPIVRFNYYSHPDDVSRCVRGVRKIGDLLRTETMERIKTRD 420
            +   S GSL L S+ + K  P VRFNY+S+P D+ RCV  + K  DLL+T++++R+K  D
Sbjct: 815  AGPLSKGSLWLASNSDVKATPHVRFNYFSNPIDLERCVSMMGKFRDLLKTKSLDRMKYND 874

Query: 421  LEGKKGFRFLGPALPSNLSDYSSVREFCRETVTSYWHYHGGCLVGKVVDGDYKVMGIKNL 480
            L+G + F F GP+LP+N +  S ++ +CR +VT++WHYHGGC VGKVVDGD+KV+GI +L
Sbjct: 875  LKGNRDFLFYGPSLPTNQTTISVIKHYCRSSVTTFWHYHGGCQVGKVVDGDFKVIGIDSL 934

Query: 481  RVVDGSTFSESPGTNPMATVMMLGR-------------------YMKFVHDANEFPEKEE 540
            RVVDGSTF  SPGTNP A++MM+GR                   YMK V++A + P  E+
Sbjct: 935  RVVDGSTFISSPGTNPQASIMMIGRYLGLKMIRERQPKTRNLVNYMKSVYNATDLPLVEK 994

Query: 541  YDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQGLFNAFATKDDGENPF 600
            YDYIVIGGGTAGCPLAAT+S K+SVL+LERG+ P  +P+ L   G       +DDG  P 
Sbjct: 995  YDYIVIGGGTAGCPLAATLSEKYSVLVLERGNVPKAHPNTLVSSGFLANLIEEDDGTTPA 1054

Query: 601  QRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWDMEMVEKAYEWVEETVV 660
            QRF SE+GVEN+RGRVLGGSSM+NA F+S    +F   +GVEWDM+ VEKAYEWV+  +V
Sbjct: 1055 QRFTSEDGVENVRGRVLGGSSMINAAFFSEADNDFLAKSGVEWDMDAVEKAYEWVKNKIV 1114

Query: 661  SEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDGEGNRHGAVELLNKAKP 720
            S  +L+ W +A + A +++GI PDNG + +H +G K  GS FD  G RHGAVELLN    
Sbjct: 1115 SYSNLSFWQAAAKEAFVEAGIGPDNGVTTKHKIGAKQSGSTFDDMGRRHGAVELLNNGNL 1174

Query: 721  TNLKIAIRATVERIIFSDLSASGVLYSDSKGRLHKALIRNKGEIMVSAGAMGSPQLLLLS 780
             NLKIAI A+                       HKALIR+KGE+++SAGA+GSPQLLLLS
Sbjct: 1175 NNLKIAIEAS----------------------SHKALIRDKGEVILSAGALGSPQLLLLS 1234

Query: 781  GVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSANIVLPFPLLPTSGKVVGILDDNIYFQ 840
            G+GPKS+LSS  +P+VL Q  VG+F++DNPR + N+++PFPL  ++G+VVGI  D  Y +
Sbjct: 1235 GIGPKSYLSSQNIPIVLSQPNVGKFMADNPRNNLNLIIPFPLEASTGQVVGITKD-YYIE 1294

Query: 841  SFAGSLPFS---LPSSF---SLLPPRSDFVDMSLAIFFGKFSKVDSVGSLRLNSSTDVKK 900
            + + SLPFS   LP  F    LLPP+     ++L +F  K     S G+L L S+TDVK 
Sbjct: 1295 TNSYSLPFSPTKLPFGFYPTPLLPPQ-----LTLTVFVEKIVGPLSTGALTLASATDVKV 1354

Query: 901  SPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIKTIDLEGKKGFRFLGSPLPENLS 960
            +P VRFNY+S+P DLARCV  ++K G+LL+TK+M++ K  D+ G + F FLG  LP N S
Sbjct: 1355 TPHVRFNYFSNPIDLARCVSAMKKFGELLKTKSMDRFKYKDMNGVRDFMFLGQLLPINQS 1414

Query: 961  DYSSVGQFCREILATYWHYHGGCLVGKVVDGDYKVMGIKNLRVVDGSTFSESPGTNPMAT 1003
            D S +  +CR  + T+WHYHGGCLVGKVVDG+++V+G  +LRVVDGSTF+ SPGTNP AT
Sbjct: 1415 DDSLMEDYCRSSVTTFWHYHGGCLVGKVVDGEFRVIGANSLRVVDGSTFTVSPGTNPQAT 1429

BLAST of Sed0018061 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 526.9 bits (1356), Expect = 3.4e-149
Identity = 252/514 (49.03%), Postives = 357/514 (69.46%), Query Frame = 0

Query: 497  YMKFVHDANEFPEKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQ 556
            +M+F+ +A +F  ++ YDYI++GGGTAGCPLAAT+S  F VLLLERG  P   P+V++  
Sbjct: 38   FMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHD 97

Query: 557  GLFNAFATKDDGENPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWD 616
            G        ++ ++P Q F+SEEGV N RGRVLGGSS +NAGFYSR  ++FFE +G+ WD
Sbjct: 98   GFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWD 157

Query: 617  MEMVEKAYEWVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDG 676
            +  V ++YEWVE  +V  P L  W +A R ALL+ G+ P NGF+L H VGTK GGS FD 
Sbjct: 158  LSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDR 217

Query: 677  EGNRHGAVELLNKAKPTNLKIAIRATVERIIF--------SDLSASGVLYSDSKGRLHKA 736
             G RH + +LL  A+ +N+++A+ ATVER++         S++SA GV+Y D  GR H A
Sbjct: 218  TGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHA 277

Query: 737  LIRNKGEIMVSAGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSANI 796
            LIR++GE+++SAGA+GSPQLL LSG+GP+S+LS+  +PV LDQ +VG F+ DNPR   +I
Sbjct: 278  LIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISI 337

Query: 797  VLPFPLLPTSGKVVGILDDNIYFQSFAGSLPFSLPSSFSLLPPRSDFVDMSLAIFFGKFS 856
            V P P+  +  +VVG+ +D  + ++ +  +PF+ P     +   +  + + +     K  
Sbjct: 338  VPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKIL 397

Query: 857  KVDSVGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMGDLLQTKTMEKIKTIDL 916
               S+G LRL +STDV+ +P+VRFNY+S P DL RCV G RK+G++L+++ M+     + 
Sbjct: 398  GPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREW 457

Query: 917  EGKKGFRFLGSPLPENLSDYSSVGQFCREILATYWHYHGGCLVGKVVDGDYKVMGIKNLR 976
             G + FRF+G+PLP + S+   +  FCR  ++T WHYHGG +VGKVVD D KV+G+ +LR
Sbjct: 458  FGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLR 517

Query: 977  VVDGSTFSESPGTNPMATLMMLGRYVGLKVLKER 1003
            +VDGSTF+ SPGTNP ATLMMLGRY+GLK+L+ER
Sbjct: 518  LVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550

BLAST of Sed0018061 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 413.7 bits (1062), Expect = 4.3e-115
Identity = 233/541 (43.07%), Postives = 318/541 (58.78%), Query Frame = 0

Query: 500  FVHDANEFPEKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQGLF 559
            F+ DA   P    YDYI+IGGGTAGCPLAAT+S   SVLLLERG  P   P++      F
Sbjct: 33   FMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERGDSPYNNPNI-TRLSAF 92

Query: 560  NAFATKDDGENPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWDMEM 619
             A  +     +P QRFVSE+GV N R RVLGG S LNAGFY+R   ++    G  WD  +
Sbjct: 93   GAALSDLSESSPSQRFVSEDGVINARARVLGGGSALNAGFYTRAGTKYVRNMG--WDGAL 152

Query: 620  VEKAYEWVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDGEGN 679
              ++Y+WVE  V  +P +  W +A R  LL++GIVP+NGF+  H+ GTK GG+IFD  GN
Sbjct: 153  ANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDHINGTKFGGTIFDRNGN 212

Query: 680  RHGAVELLNKAKPTNLKIAIRATVERIIFSDLS-----ASGVLYSDSKGRLHKALIRN-- 739
            RH A +LL  A P  + + + ATV RI+F         A+GV+Y D  G+ H+A ++   
Sbjct: 213  RHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVVYRDRTGQAHRAYLKEGA 272

Query: 740  KGEIMVSAGAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSANIVLPF 799
              EI++SAG +GSPQLL+LSGVGP + L +  + VV+DQ +VGQ + DNP  +  +  P 
Sbjct: 273  LSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMYDNPMNAVFVPSPV 332

Query: 800  PLLPTSGKVVGILDDNIYFQSFAG----------SLPFSLPSSFSLLPPRSDFVD----- 859
            P+  +  +VVGI  +  Y ++  G          S   S    +++  PR+  ++     
Sbjct: 333  PVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDYYAMFSPRATLLESNSMT 392

Query: 860  --MSLAIFFGKF--SKVD---SVGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGVRK 919
               S   F G F   KV    S G L L  + + K +P+V FNY+ HPDDL RCVRG++ 
Sbjct: 393  KLSSAQPFQGGFLLEKVMGPLSTGHLEL-KTRNPKDNPIVTFNYFQHPDDLKRCVRGIQT 452

Query: 920  MGDLLQTKTMEKIKTIDLEGKKGFRFLGSPLPENL---------SDYSSVGQFCREILAT 979
            +  ++Q+K   + K  D+  +       S  P NL         S   S  +FC+  + T
Sbjct: 453  IERVVQSKAFSRYKYADVSFEYLLNLTAS-TPVNLRPPRSGPGASLPPSAEEFCQHTVTT 512

Query: 980  YWHYHGGCLVGKVVDGDYKVMGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLKE 1003
             WHYHGGC+VG+VVDGDYKV+GI  LRV+D ST    PGTNP AT+MMLGRY+G+K+L+E
Sbjct: 513  IWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTNPQATVMMLGRYMGVKILRE 568

BLAST of Sed0018061 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 396.4 bits (1017), Expect = 7.0e-110
Identity = 218/541 (40.30%), Postives = 316/541 (58.41%), Query Frame = 0

Query: 509  EKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDP--NKYPSVLNEQGLFNAFATKD 568
            +   YDYIVIGGGTAGCPLAAT+S  FSVL+LERG  P  N   S L     F+      
Sbjct: 59   QDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADI 118

Query: 569  DGENPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWDMEMVEKAYEW 628
               +  Q FVS +GV N R RVLGG S +NAGFYSR    F + AG  WD ++V+++Y W
Sbjct: 119  SASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPW 178

Query: 629  VEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDGEGNRHGAVEL 688
            VE  +V +P L  W  A R +LL+ G+ P NGF+  H+ GTK GG+IFD  G RH A EL
Sbjct: 179  VEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAEL 238

Query: 689  LNKAKPTNLKIAIRATVERIIFSDLS----ASGVLYSDSKGRLHKALIRNK--GEIMVSA 748
            L  A P  L++ I ATV++I+F         +GV++ D KG  H+AL+ N+   E+++S+
Sbjct: 239  LAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSS 298

Query: 749  GAMGSPQLLLLSGVGPKSHLSSLKLPVVLDQQYVGQFLSDNPRFSANIVLPFPLLPTSGK 808
            GA+GSPQ+L+LSG+GPK  L  LK+PVVL+ ++VG+ ++DNP  +  +    P+  +  +
Sbjct: 299  GAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQ 358

Query: 809  VVGILDDNIYFQSFA--GSLPFSLPSSFSLLPPRSDFVD--------------------- 868
             VGI    +Y ++    G  P S+ + + ++  +++                        
Sbjct: 359  TVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKY 418

Query: 869  -----MSLAIFFGKFSKVDSVGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGVRKMG 928
                  + +    K +   S G L L  +T+V  +P V FNY+ HP DL RCV  +R + 
Sbjct: 419  QLHEAFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVS 478

Query: 929  DLLQT-----------KTMEKIKTIDLEGKKGFRFLGSPLPENLSDYSSVGQFCREILAT 988
             ++ +           + + K+ ++ ++     R      P+ L+D  S+ QFC++ + T
Sbjct: 479  KVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLR------PKQLNDTKSMAQFCKDTVVT 538

Query: 989  YWHYHGGCLVGKVVDGDYKVMGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLKE 1003
             WHYHGGCLVGKVV  + KV+G+  LRV+DGSTF ESPGTNP AT+MM+GRY+G+K+L+E
Sbjct: 539  IWHYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRE 587

BLAST of Sed0018061 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 387.1 bits (993), Expect = 4.3e-107
Identity = 229/545 (42.02%), Postives = 323/545 (59.27%), Query Frame = 0

Query: 495  GRYMKFVHDANEFPEKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPNKYPSVLN 554
            G Y +F+ DA   P+   +DYI+IGGGTAGC LAAT+S   +VL+LERG  P   P+   
Sbjct: 29   GNY-RFMKDATLAPKLSHFDYIIIGGGTAGCALAATLSQNATVLVLERGGSPYDDPAA-T 88

Query: 555  EQGLFNAFATKDDGENPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVE 614
            + G F          +  Q F+SE+GV N R RVLGG +++NAGFYSR   +F   AG  
Sbjct: 89   DIGNFANTLLNITPNSWSQLFISEDGVFNSRARVLGGGTVINAGFYSRAEEDFVAEAG-- 148

Query: 615  WDMEMVEKAYEWVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIF 674
            W+ + VE AYEWVE+ VV EP +N W SAFR  LL++G+ P NGF+  H+VGTK GG+IF
Sbjct: 149  WERDEVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLEAGVTPYNGFTYEHIVGTKFGGTIF 208

Query: 675  DGEGNRHGAVELLNKAKPTNLKIAIRATVERIIFS-----DLSASGVLYSDSKGRLHKAL 734
            D +G+RH A  LL  A P  + + + A+V +I+F+        A GV++ D+ G  +KA 
Sbjct: 209  DRDGHRHTAANLLEYANPNMIVVYLHASVHKILFTIKGNQRPKAYGVIFLDANGVSYKAE 268

Query: 735  IRNK----GEIMVSAGAMGSPQLLLLSGVGPKSHLSSLKL-PVVLDQQYVGQFLSDNPRF 794
            +  +     E+++SAGA+ SPQLL+LSGVGP +HL++ ++ PV++DQ  VGQ + DNP  
Sbjct: 269  LATQDSTMSEVILSAGAIASPQLLMLSGVGPAAHLAAYRVNPVIVDQPMVGQGMGDNPMN 328

Query: 795  SANIVLPFPLLPTSGKVVGILDDNIYFQ-SFAGSLPFSLPSSF----------SLLPPRS 854
               I  P P+  +  + VGI     Y +   A SL  SL  SF          + LP +S
Sbjct: 329  PVFIPSPEPVEVSLVQAVGITKFGSYIEGGSALSLSISLTRSFFDGVLNLLKKTKLPTQS 388

Query: 855  -----DFVDMSL------AIFFGKFSKVDSVGSLRLNSSTDVKKSPLVRFNYYSHPDDLA 914
                   +D++L       +   K +   S G L L  +T+   +P V FNY+  P+DL 
Sbjct: 389  ISKFFKSLDLTLNVTTKAGVIIQKVNGPLSRGHLELR-NTNPDDNPSVTFNYFKDPEDLN 448

Query: 915  RCVRGVRKMGDLLQTKTMEKIKTIDLEGKKGFRFLGSPLPENL-----SDYSSVGQFCRE 974
            +CV G+  +  ++ +K   K K   L   +G   L   LP NL     +    + Q+C +
Sbjct: 449  KCVEGLSTIIKVIDSKGYSKYK-YPLASARGLLNLILALPTNLRPRHITSTFDLEQYCID 508

Query: 975  ILATYWHYHGGCLVGKVVDGDYKVMGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLK 1003
             + T +HYHGGC VGKVVD +YKV+G+  LR++DGSTF +SPGTNP AT+MMLGRY+G K
Sbjct: 509  TVMTIYHYHGGCQVGKVVDNNYKVLGVDALRIIDGSTFLKSPGTNPQATIMMLGRYMGQK 567

BLAST of Sed0018061 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 386.3 bits (991), Expect = 7.3e-107
Identity = 223/528 (42.23%), Postives = 314/528 (59.47%), Query Frame = 0

Query: 500 FVHDANEFPEKEEYDYIVIGGGTAGCPLAATVSSKFSVLLLERGSDPNKYPSVLNEQGLF 559
           F+ DA   P+   +DYI+IGGGTAGC LAAT+S   SVL+LERG  P + P+  +     
Sbjct: 60  FMKDATLAPKNASFDYIIIGGGTAGCALAATLSQNASVLVLERGGSPYENPTATDMGNSV 119

Query: 560 NAFATKDDGENPFQRFVSEEGVENIRGRVLGGSSMLNAGFYSRGHREFFETAGVEWDMEM 619
           N     +   +  Q F+SE+GV N R RVLGG S++N GFYSR   ++ E A  EW+ME 
Sbjct: 120 NTL-LNNTPNSWSQLFISEDGVYNTRPRVLGGGSVINGGFYSRAGNDYVEEA--EWEMEE 179

Query: 620 VEKAYEWVEETVVSEPSLNAWHSAFRSALLQSGIVPDNGFSLRHLVGTKTGGSIFDGEGN 679
           VE AYEWVE+ +V EP +  W  AF+  LL++G  PDNGF+  H+ GTK GG+IFD  G+
Sbjct: 180 VEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEAGESPDNGFTYDHIYGTKIGGTIFDRAGH 239

Query: 680 RHGAVELLNKAKPTNLKIAIRATVERIIFSDLSASGVLYSDSKGRLHKALIRNK--GEIM 739
           RH A  LL  A P  + + + A+V +++F+   A  VL+ D+ G  HKA + NK   E++
Sbjct: 240 RHTAANLLEYANPNRIVVYLHASVHKVLFT-TEAYEVLFEDANGVFHKANLANKATNEVI 299

Query: 740 VSAGAMGSPQLLLLSGVGPKSHLSSLKL-PVVLDQQYVGQFLSDNPRFSANIVLPFPLLP 799
           +SAGA+GSPQLL+LSGVGP  HL +  + P+VLDQ  VGQ ++DNP     I  P P+  
Sbjct: 300 LSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADNPMNFVAIPSPQPVEL 359

Query: 800 TSGKVVGILDDNIYFQSFAG-SLPFSLPSSF-----SLLPPRS-----DFVDMSLAIFFG 859
           +  + VGI   + Y +  +G SL F +   F     +LL   S       +  S+A+   
Sbjct: 360 SLIQAVGITKFDSYIEGLSGLSLSFDITRRFFDGVLNLLNETSHTTSRKILTQSIAVLLK 419

Query: 860 K--------------FSKVD---SVGSLRLNSSTDVKKSPLVRFNYYSHPDDLARCVRGV 919
                          F KVD   S G ++L  +T+ + +P V FNYY  P+DL +CV+G+
Sbjct: 420 SFDVKLEVRMNGGLIFQKVDGPASKGHMKLR-NTNPRDNPSVTFNYYQEPEDLNKCVKGL 479

Query: 920 RKMGDLLQTKTMEKIKTIDLEGKKGFR-FLGSPL---PENLSDYSSVGQFCREILATYWH 979
             +  ++ +K   K K   +  ++     L  P+   P +++   ++ QFC + + + WH
Sbjct: 480 NTIIRMINSKAFSKYKYPGVTARELLNLMLALPINLRPRHVTSAFNLKQFCIDTVTSVWH 539

Query: 980 YHGGCLVGKVVDGDYKVMGIKNLRVVDGSTFSESPGTNPMATLMMLGR 993
           YHGGC VGKVVD +YKV+GI  LRV+DGSTF +SPGTNP AT+MMLGR
Sbjct: 540 YHGGCQVGKVVDKNYKVLGIDGLRVIDGSTFLKSPGTNPQATVMMLGR 582