Sed0018041 (gene) Chayote v1

Overview
NameSed0018041
Typegene
OrganismSechium edule (Chayote v1)
DescriptionCytochrome P450
LocationLG01: 21657670 .. 21662375 (+)
RNA-Seq ExpressionSed0018041
SyntenySed0018041
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATCCTTGGGAGCTATATGCTTTTACATTCCAGCGTTCTTTGTGTTTTATGTTTTAACAGTCCAGTTTCTTCACAAGATTAGAAACCTCCCCCCTACCCCTTTCCCCTCCCTCCCAATCATCGGCCATCTCCACCTCCTCAAGAAGCCCATCTATCGAACCCTGACCGACATCTCGAACCGCTACGGCCCTGTCATGTTTCTCCACTTCGGGTCGCGCCCGGTTCTCGTGGTGTCCTCCCCCGCAGCGGCGGAAGAATGTCTCAGCAAGAACGACATCGTGTTTGCGAACCGGCCTCGCCTTCTCCTATCCAAGTGGTTCGGATACAACAACACCAGCCTCGTGTGGGCGCCGTATGGAGACCACTGGCGCAACCTCCGCCGTATCTCCACCGTTGAAGTTCTGTCCACCCATCGCCTCCAGATGCTCTCGGCCGTCCGCCTCGACGAGGTTAAATCGTTGCTTCGGAGGCTCGCGAGAGGTGATGAAAACCAAGTGCATGACATGAACGCTATGCTGTTTAGTTTGACCTTCAACGTGATGTTGAGGATGATCGTGGGAAAGCGTTTCTATGGCGATGACGTGGAGGATGATGACGAGGCAAAGAGATTCAGGGAGCTGCAGACCGAGTCAACGCAGTTGAGCGGGAAGCCGAATATGAGAGACTTTATCCCGTTGATGTCGTGGCTGGGACTTGGGTCTACGTTGGAAAATAAAATAATGAAATGTCACAAAAGGAGAGATGCTTTCATGCAGAATCTAATCGACCAACAAAGAAAAAGAAAGATGGATGCTGAAACTGGAAGAGCAGAGTATATATTTCGAGATGGAGTGAAAAAAACGATGATGGAAGTATTATTGGAGCTCCAAGAATCGGAACCTCAACACTATAATGACCAAACAATCAAAGCCTTGATGTTGGTAATCACTTTTCTTCATCTCTTTAATATTATATTTAATTTTTATTTCTTAAATTCTGTTTATTCTTATTATTTGTCTCATAAAAAAAGTTTCTAAATCATCAAAAGTGCCATGTTCAAGCTTTTTAAATAACCTTTTTCGTTTTTCATTTCATTTTTGCATTTGTATTTGCTTTTTTCCAATAATTTATTTATTAATACATAATACTATTTATGATACCTTATATTAATAATTATGGATATAAATATTTTAAAATTTAACTACATTTATAATTATTTTGTAAAAGCATACAATTTTCCAATAATTTATTAATTCAAAGCTATAATTTTTTTTTTGTACATAATATCTTGTATTTTTAAGAAATGGATATTTGAAAAATAAGTTAAGGAATTATATTTCGGTTAATAATTTTCTGCTTAAAAAAACGTTAAAAATAGTTTTTATAATGAATGAGTATTTTACTATACATATATATATTTTTTAAAAAGAAAATAATATTATGTAAAAATAAATATGATTTAGATAAGAAATAAAAATAGAAAGAAAAATAAATGTTATAATATAAAAAAAATATATAGTTGACATTATCCACAATTGTATTATTATTTTTTAAAATTTTCTTCTCTATTTTCTAGGTTAAGAAATTTAAAAAAAAAAAATTCTCACTATGTTCTTTTAAAGTGTATAAATTTCAATTAGTTGAAAATTATACTATATAGATAGAAACTAAAATAAACATTATAAAATTTAATTATATTGATACTGTAAGACTAAAATGTTATTTTGGAATAGTACTAAAAAAATGTTATTTTGGAATATGTATATGATTACTTAAATGAAAAAAAGAAGAAAATAAAAATACAAAATAAAAATAAATAAGAAACGATAAATTTTCTTTATTTTTTATTTTACAGATGGCGACACATGACACTATAAGATACGTGTACATGTGGTAAATCATGGTGCAAGCCAGGTTGCACCAAGTTTTGCTAAAAATAATGTACCGCTACGTTTCAGAAAAAAAATAAGAGTCTCACTAAATATATAAAATCAAATATTTTTATTAATAAAATTTAGGTTAAAATATTATTTTAGCCTTAGGTTTATGGTTGCTTTGCTTACGTAAATTTTTATTTCATTTTAATCTATCTATTTTCAAATCTTCAATTTTAGTACATTTTAATTCTTTGAACACAAGTTTGAACTTGAAAATTGGTATTCAACTCTCATATGATTTGAGAGCAGTTGTGCTTCTTATTAGGGGTGCAACGAAAAATCGTAAAACCAAAAAATTGAACCGAATCAATCCAATCCAATAATGGATCGAATCAATTTTTTAACACAAAATTGGACATATCGATTTGTAATTTGAAAAAACCGAAAATATTGGATCGGTTTGAATAAGTTACAAATATTTCTAACCTTTTAAACAAATAGTTGTTTATGAGAAGTTTCTTTTTATATGAGAAATATTGTTTGTTATATATTAAAAAAAAATTGGACATTGGATCGGATCAGATCCATTTTTGAAAATTGGAAAATCGAACCAATGATTCTATACTTTATTGGACATTGGATCAAATCCATTTTTTAAAAACCGAAGATATCGGTTCGGTTGTCGGTTTGATACAAAACCAACAAAAACTGAACCGAAATCATCCTTACTTCCTATTTGTTGATCCCATTCAAAAAAAAAATCTTCAATTTTAATCTTTTAATTTTTCGAATTTTTGTTAAATAGTCTATGTAATTAAAAAGGGAAAAAAAAAGTTATGTTTTTTTTTGTAAGTACAGAGTGAAAGGCCATAATAGGAAAAATAAAAGTCCATAAGTGATGAAGAAACCATGGAAAGTGAAATTTCTCTCAACAACAACAATACCTTAATAAACTCTGATCTCTACGCTGCAATTTTAACAAATATTTTGTCAAATAATTACCTATTAACAAAAAGGGGGAAAAGAGGAAAAATAAAAGTGTAGGTTTAGAAATTGGAGAAGGGATTGATTAAGAAAAAGAGGAAATAATTTCAGTGCTTTCAAAAACTAAATTGAGCAACAAAATCATGTAATTGGATTGATTGAGAAAAGTGTAGTTTAGAAATTGGGGAAGGGATGAATATAAACAAAAAGGGGAAAAAGAGGAGAAATAATAAAGATGAATTCAAAACAGTTTGTTTTTAAATGAGAACAAAATCTCTATGAGTCCATGACTCTATCCAAACCTCCAAACAATAAGGAATTTAAGGCAATATTGTTTTTTTTTTAAAAAAATATGAAGCTCACTTACCAGGGTTTCTTCTTCACTTTTTGGCTTTTTATTTCCCTCTTGGTCTTTCACCTGTACAGAAAAAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAGAAAGAAACAGAGCACAAACTAAAGAAGGAAGAAGGGAGAGTATTATTATTACTATTATTATTATTATTTTCCCTATCTTTAATGTTTTTATCGTAATCACAAGACTATTTTTAAAAAAAAAAAATTGAAAAGTTTAAAAGTCAAAATTGGATATTTGTAAAGTGAGAGATCAAAATTAGACATAAAAATAGATTAAAATAGGCATTTAAAATTTAATCGACCAAAATTAAAATAACAAAAATATAGTTTAATAACAAAATGCGGACTAAAATAATAATTAGATAAATTTTAATTTCTAATATATTTATATGGTTATTTTAATTTTAAAGAGCAAAATAAGTTTAAAAAAATAATATAAACCAAAACAGTAAAAAGACTAAAATTGAGTCAATATAAAAATTACATCCAAAATGTAGCTACAAGTAAGTGTTTTTTTTTAATTTTAAAAATACCTAAATTATAACCAAATGGAGGAGTGATAGTAATGATTTCATTGTCCGGTTATAAACCAGTTCAACACCAACGTCAAACGTTTGATCTAGAAAATCGGTATTTGTCCCCAACTTTGTTTCTAAGAAATTTAAAATAAAGTTTTGTTAGAATAATAAACTGAATACAAAACTAATATTTACTAATTTGTCAATTAAAATATGAAAAGGTTCAAACATTTTAAAGGGATTTTGGATTACAAAATTAATGTGTACTGTGTACATGTACAGGTTTTGTTAGCTGCAGGTACCGAAACGTCTGTGAACACAATGGAATGGGGCCTCTCTCTTTTGCTAAACCACCCGGAAGTTTTACAGAAAGCTCAGCTCGAAATCGACACCAAAGTTGGCAACGACCACTTGATGAACGAATCCGACGTGGCCAAACTCCCCTTCCTACGTGGCATCATCAACGAGACTCTACGAATGTACCCTCCAGCTCCACTCATGGTACCTCATGAGTCATCAAAGGATTGCACTGTCGGAGGTTATCACATCCCACGTGGCACCATGCTGTACGTCAACTTATGGGCCATCCATAACCACCCTACGATTTGGGCCCACCCGAGAAAGTTTGACCCCGACCGGTTTGAGAGGCTGGGGAGAGAAAAGTTTGGGTTTAAGCTGATGCCGTTCGGGTCGGGTCGAAGGGGGTGCCCGGGAGAGGGGCTGGGCCTTCGGGTTATAGGCCTTGTACTGGGCTCCTTGATCCAGTGCTTTCAATGGGAGCGTCCTGGTGAAGACTTGGTGGATATGACCGAAGGCATTGCTGTCTCCATGCCTAAGGCCCGACGCCTGCAGGCTAAGTGTAAGCCTCGCTCTATACTCGATCGAGTCGATCTTTCTCAAACCCAACACAACCCGGACCGACTAGTCTGCTGCGTAAGTTTTGGTGCAATGCAACTAATCTAG

mRNA sequence

ATGAGATCCTTGGGAGCTATATGCTTTTACATTCCAGCGTTCTTTGTGTTTTATGTTTTAACAGTCCAGTTTCTTCACAAGATTAGAAACCTCCCCCCTACCCCTTTCCCCTCCCTCCCAATCATCGGCCATCTCCACCTCCTCAAGAAGCCCATCTATCGAACCCTGACCGACATCTCGAACCGCTACGGCCCTGTCATGTTTCTCCACTTCGGGTCGCGCCCGGTTCTCGTGGTGTCCTCCCCCGCAGCGGCGGAAGAATGTCTCAGCAAGAACGACATCGTGTTTGCGAACCGGCCTCGCCTTCTCCTATCCAAGTGGTTCGGATACAACAACACCAGCCTCGTGTGGGCGCCGTATGGAGACCACTGGCGCAACCTCCGCCGTATCTCCACCGTTGAAGTTCTGTCCACCCATCGCCTCCAGATGCTCTCGGCCGTCCGCCTCGACGAGGTTAAATCGTTGCTTCGGAGGCTCGCGAGAGGTGATGAAAACCAAGTGCATGACATGAACGCTATGCTGTTTAGTTTGACCTTCAACGTGATGTTGAGGATGATCGTGGGAAAGCGTTTCTATGGCGATGACGTGGAGGATGATGACGAGGCAAAGAGATTCAGGGAGCTGCAGACCGAGTCAACGCAGTTGAGCGGGAAGCCGAATATGAGAGACTTTATCCCGTTGATGTCGTGGCTGGGACTTGGGTCTACGTTGGAAAATAAAATAATGAAATGTCACAAAAGGAGAGATGCTTTCATGCAGAATCTAATCGACCAACAAAGAAAAAGAAAGATGGATGCTGAAACTGGAAGAGCAGAGTATATATTTCGAGATGGAGTGAAAAAAACGATGATGGAAGTATTATTGGAGCTCCAAGAATCGGAACCTCAACACTATAATGACCAAACAATCAAAGCCTTGATGTTGGTTTTGTTAGCTGCAGGTACCGAAACGTCTGTGAACACAATGGAATGGGGCCTCTCTCTTTTGCTAAACCACCCGGAAGTTTTACAGAAAGCTCAGCTCGAAATCGACACCAAAGTTGGCAACGACCACTTGATGAACGAATCCGACGTGGCCAAACTCCCCTTCCTACGTGGCATCATCAACGAGACTCTACGAATGTACCCTCCAGCTCCACTCATGGTACCTCATGAGTCATCAAAGGATTGCACTGTCGGAGGTTATCACATCCCACGTGGCACCATGCTGTACGTCAACTTATGGGCCATCCATAACCACCCTACGATTTGGGCCCACCCGAGAAAGTTTGACCCCGACCGGTTTGAGAGGCTGGGGAGAGAAAAGTTTGGGTTTAAGCTGATGCCGTTCGGGTCGGGTCGAAGGGGGTGCCCGGGAGAGGGGCTGGGCCTTCGGGTTATAGGCCTTGTACTGGGCTCCTTGATCCAGTGCTTTCAATGGGAGCGTCCTGGTGAAGACTTGGTGGATATGACCGAAGGCATTGCTGTCTCCATGCCTAAGGCCCGACGCCTGCAGGCTAAGTGTAAGCCTCGCTCTATACTCGATCGAGTCGATCTTTCTCAAACCCAACACAACCCGGACCGACTAGTCTGCTGCGTAAGTTTTGGTGCAATGCAACTAATCTAG

Coding sequence (CDS)

ATGAGATCCTTGGGAGCTATATGCTTTTACATTCCAGCGTTCTTTGTGTTTTATGTTTTAACAGTCCAGTTTCTTCACAAGATTAGAAACCTCCCCCCTACCCCTTTCCCCTCCCTCCCAATCATCGGCCATCTCCACCTCCTCAAGAAGCCCATCTATCGAACCCTGACCGACATCTCGAACCGCTACGGCCCTGTCATGTTTCTCCACTTCGGGTCGCGCCCGGTTCTCGTGGTGTCCTCCCCCGCAGCGGCGGAAGAATGTCTCAGCAAGAACGACATCGTGTTTGCGAACCGGCCTCGCCTTCTCCTATCCAAGTGGTTCGGATACAACAACACCAGCCTCGTGTGGGCGCCGTATGGAGACCACTGGCGCAACCTCCGCCGTATCTCCACCGTTGAAGTTCTGTCCACCCATCGCCTCCAGATGCTCTCGGCCGTCCGCCTCGACGAGGTTAAATCGTTGCTTCGGAGGCTCGCGAGAGGTGATGAAAACCAAGTGCATGACATGAACGCTATGCTGTTTAGTTTGACCTTCAACGTGATGTTGAGGATGATCGTGGGAAAGCGTTTCTATGGCGATGACGTGGAGGATGATGACGAGGCAAAGAGATTCAGGGAGCTGCAGACCGAGTCAACGCAGTTGAGCGGGAAGCCGAATATGAGAGACTTTATCCCGTTGATGTCGTGGCTGGGACTTGGGTCTACGTTGGAAAATAAAATAATGAAATGTCACAAAAGGAGAGATGCTTTCATGCAGAATCTAATCGACCAACAAAGAAAAAGAAAGATGGATGCTGAAACTGGAAGAGCAGAGTATATATTTCGAGATGGAGTGAAAAAAACGATGATGGAAGTATTATTGGAGCTCCAAGAATCGGAACCTCAACACTATAATGACCAAACAATCAAAGCCTTGATGTTGGTTTTGTTAGCTGCAGGTACCGAAACGTCTGTGAACACAATGGAATGGGGCCTCTCTCTTTTGCTAAACCACCCGGAAGTTTTACAGAAAGCTCAGCTCGAAATCGACACCAAAGTTGGCAACGACCACTTGATGAACGAATCCGACGTGGCCAAACTCCCCTTCCTACGTGGCATCATCAACGAGACTCTACGAATGTACCCTCCAGCTCCACTCATGGTACCTCATGAGTCATCAAAGGATTGCACTGTCGGAGGTTATCACATCCCACGTGGCACCATGCTGTACGTCAACTTATGGGCCATCCATAACCACCCTACGATTTGGGCCCACCCGAGAAAGTTTGACCCCGACCGGTTTGAGAGGCTGGGGAGAGAAAAGTTTGGGTTTAAGCTGATGCCGTTCGGGTCGGGTCGAAGGGGGTGCCCGGGAGAGGGGCTGGGCCTTCGGGTTATAGGCCTTGTACTGGGCTCCTTGATCCAGTGCTTTCAATGGGAGCGTCCTGGTGAAGACTTGGTGGATATGACCGAAGGCATTGCTGTCTCCATGCCTAAGGCCCGACGCCTGCAGGCTAAGTGTAAGCCTCGCTCTATACTCGATCGAGTCGATCTTTCTCAAACCCAACACAACCCGGACCGACTAGTCTGCTGCGTAAGTTTTGGTGCAATGCAACTAATCTAG

Protein sequence

MRSLGAICFYIPAFFVFYVLTVQFLHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDISNRYGPVMFLHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDHWRNLRRISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGDENQVHDMNAMLFSLTFNVMLRMIVGKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMKCHKRRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTIKALMLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPFLRGIINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKFDPDRFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDMTEGIAVSMPKARRLQAKCKPRSILDRVDLSQTQHNPDRLVCCVSFGAMQLI
Homology
BLAST of Sed0018041 vs. NCBI nr
Match: XP_022940352.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 844.3 bits (2180), Expect = 5.6e-241
Identity = 411/515 (79.81%), Postives = 460/515 (89.32%), Query Frame = 0

Query: 4   LGAICFYIPAFFVFYVLTVQFLHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDISNRY 63
           +GAI  YIP FFV YVLT QFLH+IRNLPPTPF SLPIIGHLHLLKKP+YRTL +ISNRY
Sbjct: 1   MGAIIVYIPLFFVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 64  GPVMFLHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDH 123
           GPV+FL FGSR VLVVSSP+AAEECLSKND+VFA+RPRLL+SKWFGYNN +LVW+ YGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 124 WRNLRRISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGDENQVHDMNAMLFSLTFNVML 183
           WRNLRRISTVE+LSTHRLQML+AVRLDEVKSL+ RLA+G ENQV DMN   F LTFNVML
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRLDEVKSLIGRLAKG-ENQVQDMNNAFFDLTFNVML 180

Query: 184 RMIVGKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMK 243
           RMIVGKRFYG+DVED DEA RFRELQ++STQL GK NM DFIPLMSWLGLG   E +IM+
Sbjct: 181 RMIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGWAAEKEIME 240

Query: 244 CHKRRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTI 303
           CH++RDAFMQNLI+Q + R ++ E   +   FRDG KKT++EVLLELQES+P  YND+TI
Sbjct: 241 CHQKRDAFMQNLINQHKNRAIETEPDNS---FRDGRKKTLIEVLLELQESDPDQYNDETI 300

Query: 304 KALMLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPF 363
           +ALMLVLLAAGTETS+NTMEW LSL+LN+PEVLQKAQLEID KVG+D LM+ESD+A +PF
Sbjct: 301 RALMLVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPF 360

Query: 364 LRGIINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKF 423
           LRGIINETLRMYPPAPLMVPHESSKDC+VGGYHIPRGTML+VNLWAIHN P IWA PRKF
Sbjct: 361 LRGIINETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKF 420

Query: 424 DPDRFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDM 483
           DPDRF ++  EK GFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCF+WERPGE+LVDM
Sbjct: 421 DPDRFVKVESEKLGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEELVDM 480

Query: 484 TEGIAVSMPKARRLQAKCKPRSILDRVDLSQTQHN 519
           TEG+AVSMPKAR LQAKCKPR I+DR+ LSQT+ N
Sbjct: 481 TEGVAVSMPKARHLQAKCKPRPIVDRLHLSQTKSN 511

BLAST of Sed0018041 vs. NCBI nr
Match: XP_022982225.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 843.6 bits (2178), Expect = 9.5e-241
Identity = 410/515 (79.61%), Postives = 458/515 (88.93%), Query Frame = 0

Query: 4   LGAICFYIPAFFVFYVLTVQFLHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDISNRY 63
           +GAI  YIP F V YVLT QF+HKIRNLPPTPF SLPIIGHLHLLKKP+YRTL +ISNRY
Sbjct: 1   MGAIILYIPLFLVLYVLTEQFIHKIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 64  GPVMFLHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDH 123
           GPV+FL FGSR VLVVSSP+AAEECLSKND+VFA+RPRLL+SKWFGYNN +LVW+ YGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 124 WRNLRRISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGDENQVHDMNAMLFSLTFNVML 183
           WRNLRRISTVE+LSTHRLQML+ VRLDEVKSL+ RLARG ENQV DMN   F LTFNVML
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAGVRLDEVKSLIGRLARG-ENQVQDMNNAFFDLTFNVML 180

Query: 184 RMIVGKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMK 243
           RMIVGKRFYG+DVED DEA RFRELQ++STQL GK NM DFIPLMSWLGLG   E +IM+
Sbjct: 181 RMIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGRAAEKEIME 240

Query: 244 CHKRRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTI 303
           CH++RDAFMQNLI+Q + R ++ E      +FRDG KKT++EVLLELQES+P  YND+TI
Sbjct: 241 CHQKRDAFMQNLINQHKNRAIETE---PHNLFRDGRKKTLIEVLLELQESDPDQYNDETI 300

Query: 304 KALMLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPF 363
           +ALMLVLLAAGTETS+NTMEW LSL+LN+PEVLQKAQLEID KVG+D LM+ESD+A +PF
Sbjct: 301 RALMLVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPF 360

Query: 364 LRGIINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKF 423
           LRGIINETLRMYPPAPLMVPHESSKDC+VGGYHIPRGTML+VNLWAIHN P IWA PRKF
Sbjct: 361 LRGIINETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKF 420

Query: 424 DPDRFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDM 483
           DPDRF ++  EK GFKL+PFGSGRRGCPGEGLGLRVIGLVLGSLIQCF+WERPGEDLVDM
Sbjct: 421 DPDRFVKVDSEKLGFKLLPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDM 480

Query: 484 TEGIAVSMPKARRLQAKCKPRSILDRVDLSQTQHN 519
           TEG+AVSMPKAR LQAKCKPR I+DR+ LSQT+ N
Sbjct: 481 TEGVAVSMPKARHLQAKCKPRPIVDRLHLSQTKSN 511

BLAST of Sed0018041 vs. NCBI nr
Match: XP_023523281.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 835.9 bits (2158), Expect = 2.0e-238
Identity = 408/515 (79.22%), Postives = 456/515 (88.54%), Query Frame = 0

Query: 4   LGAICFYIPAFFVFYVLTVQFLHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDISNRY 63
           +GAI  YIP F V YVLT QFLH+IRNLPPTPF SLPIIGHLHLLKKP+YRTL +ISNRY
Sbjct: 1   MGAIIVYIPLFLVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 64  GPVMFLHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDH 123
           GPV+FL FGSR VLVVSSP+AAEECLSKND+VFA+RPRLL+SKWFGYNN +LVW+ YGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 124 WRNLRRISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGDENQVHDMNAMLFSLTFNVML 183
           WRNLRRISTVE+LSTHRLQML+AVR DEVKSL+ RLARG ENQV DMN   F LTFNVML
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRSDEVKSLIGRLARG-ENQVQDMNNAFFDLTFNVML 180

Query: 184 RMIVGKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMK 243
           RMIVGKRFYG+DVED DEA RFRELQ++STQL GK NM DFIPLMSWLGLG   E +IM+
Sbjct: 181 RMIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMEDFIPLMSWLGLGWAAEKEIME 240

Query: 244 CHKRRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTI 303
           CH++RDAFMQNLI+Q + R ++ E   +   FRDG KKT++EVL+ELQES+P  YND+TI
Sbjct: 241 CHQKRDAFMQNLINQHKNRAIETEPDNS---FRDGRKKTLIEVLVELQESDPDQYNDETI 300

Query: 304 KALMLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPF 363
           +ALMLVLLAAGTETS+NTMEW LSL+LN+PEVLQKAQLEID KVG+D LM+ESD+A +PF
Sbjct: 301 RALMLVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPF 360

Query: 364 LRGIINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKF 423
           LRGIINETLRMYPPAPLMVPHESSKDC+VGGY IPRGTML+VNLWAIHN P IWA PRKF
Sbjct: 361 LRGIINETLRMYPPAPLMVPHESSKDCSVGGYQIPRGTMLFVNLWAIHNDPKIWADPRKF 420

Query: 424 DPDRFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDM 483
           DPDRF ++  EK GFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCF+WERPGEDLVDM
Sbjct: 421 DPDRFVKVDSEKHGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDM 480

Query: 484 TEGIAVSMPKARRLQAKCKPRSILDRVDLSQTQHN 519
           TEG+AVSMPKAR LQAKCKPR I+ R+ LSQT+ N
Sbjct: 481 TEGVAVSMPKARHLQAKCKPRPIVARLHLSQTKSN 511

BLAST of Sed0018041 vs. NCBI nr
Match: XP_022146589.1 (cytochrome P450 81D1-like [Momordica charantia])

HSP 1 Score: 779.6 bits (2012), Expect = 1.7e-221
Identity = 378/522 (72.41%), Postives = 446/522 (85.44%), Query Frame = 0

Query: 1   MRSLGAICFYIPAFFVFYVLTVQFLHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDIS 60
           + ++GAI  Y P F V Y+LT  FLHKIRNLPPTPFPSLP+IGHL+LL KPIYRTL DIS
Sbjct: 6   LAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADIS 65

Query: 61  NRYGPVMFLHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPY 120
           NR+GPV+FL FGSRPVLVVSSP+ AEECL+KND+VFANRPRLL+SK FGYNNT+LVW+ Y
Sbjct: 66  NRHGPVVFLRFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSY 125

Query: 121 GDHWRNLRRISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGD-ENQVHDMNAMLFSLTF 180
           GDHWR+LRRISTVEVLSTHRLQ+LS VRL+EV+SL+R+LA+ + ++++  M    F LTF
Sbjct: 126 GDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTF 185

Query: 181 NVMLRMIVGKRFYGDDVED-DDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLE 240
           NVMLRMI GKRFYGDDV+D D++AK FRELQ EST+L+G  N+ DF+P+M+WLG G ++E
Sbjct: 186 NVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVE 245

Query: 241 NKIMKCHKRRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHY 300
            K+M CH +RDAFMQ LI++QRKR         +  FRDG +KTM+EVLLELQESEP HY
Sbjct: 246 KKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHY 305

Query: 301 NDQTIKALMLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDV 360
            D++IKALMLVLL AGTETSVNTMEW LSLLLNHPEVL+KAQ+EID +VG D L++ESD+
Sbjct: 306 TDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDBQVGQDRLIDESDM 365

Query: 361 AKLPFLRGIINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWA 420
           A LP+LRGII ET RMYPPAPLMVPH+SS+DC+VGG+HIPRGTMLYVNLWAIHN P IWA
Sbjct: 366 AHLPYLRGIIXETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWA 425

Query: 421 HPRKFDPDRFERLGREK--FGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERP 480
           +P KFDPDRF+RL  +K  +GF LMPFGSGRRGCPGEGLGLR+IGLVLGSLIQCF+WERP
Sbjct: 426 NPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERP 485

Query: 481 GEDLVDMTEGIAVSMPKARRLQAKCKPRSILDRVDLSQTQHN 519
           GE LVDMTEGIAVSMPKA+ LQAKC+PR I+  +  SQT+ N
Sbjct: 486 GEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKTN 526

BLAST of Sed0018041 vs. NCBI nr
Match: XP_008453068.1 (PREDICTED: cytochrome P450 81D1-like [Cucumis melo])

HSP 1 Score: 775.8 bits (2002), Expect = 2.4e-220
Identity = 373/519 (71.87%), Postives = 435/519 (83.82%), Query Frame = 0

Query: 1   MRSLGAICFYIPAFFVFYVLTVQFLHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDIS 60
           M  LG+I  Y P F V Y+L    LHKIRNLPPTPFP LPIIGHLHLLKKPIYRTL  IS
Sbjct: 1   MGVLGSIFVYFPLFLVLYILIEHLLHKIRNLPPTPFPLLPIIGHLHLLKKPIYRTLAKIS 60

Query: 61  NRYGPVMFLHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPY 120
           N YGPV++  FGSR VLVVSS  AAEECL+KND+VFANRPRL++SKWFGYNNT+L+W+ Y
Sbjct: 61  NHYGPVVYFRFGSRKVLVVSSSFAAEECLTKNDVVFANRPRLIISKWFGYNNTNLIWSSY 120

Query: 121 GDHWRNLRRISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGDENQVHDMNAMLFSLTFN 180
           GDHWRNLRRIST+E+LSTHRLQM S+VRL+EVKSL+RRLA  DENQ+H+M    F LT+N
Sbjct: 121 GDHWRNLRRISTIEILSTHRLQMFSSVRLEEVKSLIRRLA-NDENQIHNMKNEFFDLTYN 180

Query: 181 VMLRMIVGKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENK 240
           +MLRM+VGKR+YG+DV+D DEAK FR+LQ +  QL GK  ++DFIPL+SW+G GSTLENK
Sbjct: 181 MMLRMLVGKRYYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPLVSWMGFGSTLENK 240

Query: 241 IMKCHKRRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYND 300
           I++ H +RD FMQNLIDQ +KR  D     +  I++DG +KTM+EVLLELQ+ EP  Y D
Sbjct: 241 IIELHVKRDTFMQNLIDQHKKRNFD--QNNSNKIYQDGRRKTMIEVLLELQQYEPNQYTD 300

Query: 301 QTIKALMLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAK 360
           +TI+ALML+LLAAGTETSV TMEW LSL+LNHPE L+K Q EID++VG+D L++ESD+A 
Sbjct: 301 ETIRALMLILLAAGTETSVVTMEWALSLMLNHPEFLKKLQNEIDSQVGHDRLIDESDMAN 360

Query: 361 LPFLRGIINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHP 420
           LP LRGIINET RMYPPAPLMVPHESSKDC VGGYHIPRGT+LYVNLWAIHN P IW +P
Sbjct: 361 LPSLRGIINETFRMYPPAPLMVPHESSKDCIVGGYHIPRGTILYVNLWAIHNDPKIWDNP 420

Query: 421 RKFDPDRFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDL 480
           RKF+P+RFE L  EKFGF LMPFGSGRRGCPGEGLGLRVIGLVLGSL+QCF+WERPGE+L
Sbjct: 421 RKFNPNRFESLESEKFGFNLMPFGSGRRGCPGEGLGLRVIGLVLGSLVQCFEWERPGEEL 480

Query: 481 VDMTEGIAVSMPKARRLQAKCKPRSILDRVDLSQTQHNP 520
           VDMTEGIA++MPKA  LQAKC PR I+ R+ LS    NP
Sbjct: 481 VDMTEGIALTMPKAHCLQAKCTPRPIVHRLHLSHKISNP 516

BLAST of Sed0018041 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 2.7e-137
Identity = 248/484 (51.24%), Postives = 328/484 (67.77%), Query Frame = 0

Query: 29  RNLPPTPFPSLPIIGHLHLLKKPIYRTLTDISNRYGPVMFLHFGSRPVLVVSSPAAAEEC 88
           RNLPP+P  +LP+IGHLHL+ K ++R+L D+S +YG V  L  G+R VLVVSSPAAAEEC
Sbjct: 30  RNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEEC 89

Query: 89  LSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDHWRNLRRISTVEVLSTHRLQMLSAVR 148
            +KNDIVFANRP  +L K+ GYN T++V +PYG+HWRNLRR++ VE+ S   L    ++R
Sbjct: 90  FTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIR 149

Query: 149 LDEVKSLLRRL--ARGDENQVHDMNAMLFSLTFNVMLRMIVGKRFYGDDVEDDDEAKRFR 208
            DEVK LL  L  + G +    +M + L  L+FNV +RM+ GKR++G DV D DEAK FR
Sbjct: 150 EDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTMRMVAGKRYFGQDV-DSDEAKLFR 209

Query: 209 ELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMKCHKRRDAFMQNLIDQQRKRKMDA 268
            L  E  + +G  N  DF+P + W+      E K+ K  +  DAF+Q LI + R  K + 
Sbjct: 210 ALIGEVFEHAGASNPGDFVPFLRWIDF-KNYEKKVSKISQEMDAFLQRLIHESRINKNNV 269

Query: 269 ETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTIKALMLVLLAAGTETSVNTMEWGL 328
                          TM++ LL LQES+P++Y DQ IK +++VLL AGT+TS  T+EW +
Sbjct: 270 ---------------TMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTDTSAVTVEWAM 329

Query: 329 SLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPFLRGIINETLRMYPPAPLMVPHES 388
           SLLLNHPE L+KA+ EI+T+VG++ L+ E D+ KL +L  II+ET R+ P AP++VPHES
Sbjct: 330 SLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCPAAPMLVPHES 389

Query: 389 SKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKFDPDRFERLGREKFGFKLMPFGSG 448
           S DC V GY +P+GT+L VN WAIH  P  W  P  F P+R    G E    KLMPFG G
Sbjct: 390 SDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHG--GVELEPSKLMPFGMG 449

Query: 449 RRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDMTEGIAVSMPKARRLQAKCKPRSI 508
           RR CPG GL  RV+GL LG+LIQCF+W+R GE  +DM EG  ++MPKA+ L+A CKPR+I
Sbjct: 450 RRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQPLEALCKPRNI 494

Query: 509 LDRV 511
           L +V
Sbjct: 510 LHKV 494

BLAST of Sed0018041 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 1.3e-131
Identity = 246/504 (48.81%), Postives = 337/504 (66.87%), Query Frame = 0

Query: 16  VFYVLTVQFLH-KIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDIS-----NRYGPVMFL 75
           +F +++ +FL  K +NLPP+P   LPIIGHL LLK PI+RTL   S     N  G VM L
Sbjct: 17  IFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDGGGVMSL 76

Query: 76  HFGSRPVLVVSS-PAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDHWRNLR 135
             GSR V VVSS   AAEEC  KND+V ANRP++++ K  GYNNT+++ APYGDHWRNLR
Sbjct: 77  RLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHWRNLR 136

Query: 136 RISTVEVLSTHRLQMLSAVRLDEVKSLLRRLAR--GDENQVHDMNAMLFSLTFNVMLRMI 195
           R+ T+E+ STHRL     VR DEV+ L+ RL+R  G +  V ++  ML  LTFN ++RM+
Sbjct: 137 RLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTFNNIMRMM 196

Query: 196 VGKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMKCHK 255
            GKR+YG++  D++EAKR R+L  +    +   N  D++P+   L L S+ EN++ K  +
Sbjct: 197 TGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPI---LRLFSSYENRVKKLGE 256

Query: 256 RRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTIKAL 315
             D F+Q LID +R ++   ETG            TM++ LL LQ+S+ ++Y DQ IK +
Sbjct: 257 ETDKFLQGLIDDKRGQQ---ETG-----------TTMIDHLLVLQKSDIEYYTDQIIKGI 316

Query: 316 MLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPFLRG 375
           +L+++ AGT TS  T+EW LS LLNHP+V+ KA+ EID +VG D L+ E+D+++LP+L+ 
Sbjct: 317 ILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKN 376

Query: 376 IINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKFDPD 435
           I+ ETLR++P  PL+VPH +S+DC +G Y +PRGT L VN WAIH  P  W  P  F P+
Sbjct: 377 IVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPE 436

Query: 436 RFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDMTEG 495
           RFE   +E+   KL+ FG GRR CPG GL  R++GL LGSLIQCF+WER G   VDM EG
Sbjct: 437 RFE---KEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEG 496

Query: 496 IAVSMPKARRLQAKCKPRSILDRV 511
           +  ++PKA  L+A CK R  L ++
Sbjct: 497 VGNTVPKAIPLKAICKARPFLHKI 500

BLAST of Sed0018041 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 454.5 bits (1168), Expect = 1.6e-126
Identity = 230/506 (45.45%), Postives = 329/506 (65.02%), Query Frame = 0

Query: 9   FYIPAFFVFYVLTVQFLHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDISNRYGPVMF 68
           FY+  FF+F +  V    K +NLPP P PSLPIIG+LH LK+P++RT   +S +YG V  
Sbjct: 12  FYLALFFIFNI--VIRARKFKNLPPGP-PSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFS 71

Query: 69  LHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDHWRNLR 128
           L FGSR V+VVSS +  ++C +KND+V ANRPR L  K+  YN T+L    YG+HWRNLR
Sbjct: 72  LWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 131

Query: 129 RISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGDENQVHDM--NAMLFSLTFNVMLRMI 188
           RI+ ++VLS HR+   S +R DE + L+ RLA        +M  ++ L+ +TFN ++RMI
Sbjct: 132 RITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMI 191

Query: 189 VGKRFYGDDVEDDD--EAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMKC 248
            GKR+YG+D +  D  EA +FR++ +E  QLSG  N  DF+PL+ +L     LE ++   
Sbjct: 192 SGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDF-ENLEKRLKDI 251

Query: 249 HKRRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTIK 308
             + DAF++ LI++ R +K  A               TM++ LL LQ+S+P++Y DQ IK
Sbjct: 252 SGKTDAFLRGLIEEHRTKKERA--------------NTMIDHLLNLQDSQPEYYTDQIIK 311

Query: 309 ALMLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPFL 368
            L L +L AGT++S  T+EW +S LLNHPEVL+K + E+DT VG D L++ESD+ KL +L
Sbjct: 312 GLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYL 371

Query: 369 RGIINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKFD 428
           + +INETLR+Y PAPL++PH +S +C +GGY +P+ T++ +N WAIH  P +W     F 
Sbjct: 372 KNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFK 431

Query: 429 PDRFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDMT 488
           P+RFE+ G  +   KL+ FG GRR CPGEGL +R I + L  LIQCF W+    D +D+ 
Sbjct: 432 PERFEKKGELE---KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLA 491

Query: 489 EGIAVSMPKARRLQAKCKPRSILDRV 511
           E    ++ K   L+A CK R ++++V
Sbjct: 492 ERDGFTLTKLVPLKAMCKSRPVINKV 496

BLAST of Sed0018041 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.6e-126
Identity = 233/500 (46.60%), Postives = 324/500 (64.80%), Query Frame = 0

Query: 11  IPAFFVFYVLTVQF--LHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDISNRYGPVMF 70
           I  FF+   L V F    K +NLPP P   LPIIG+LH LK+P++ T   +S +YG +  
Sbjct: 10  ISLFFLIITLKVFFNTSRKFKNLPPGP-QCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFS 69

Query: 71  LHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDHWRNLR 130
           L FGSR V+VVSS   A+EC +KNDIV ANRP  L  K+ GYNNT++  +PYGDHWRNLR
Sbjct: 70  LWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLR 129

Query: 131 RISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGDENQVH--DMNAMLFSLTFNVMLRMI 190
           RI ++E+LS+HRL     +R DE+  L+++LA+   N     ++  M   +TFN ++RM+
Sbjct: 130 RILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMV 189

Query: 191 VGKRFYGD--DVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMKC 250
            GKR+YG+  DV D +EA+ FR +  E   L G  N+ DF+  + W      LE ++ K 
Sbjct: 190 SGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDG-LEKRLKKI 249

Query: 251 HKRRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTIK 310
            KR DAF+Q LID+ R  K ++               TM++ LL  Q+S+P++Y DQ IK
Sbjct: 250 SKRTDAFLQGLIDEHRFGKRNS--------------NTMIDHLLTQQQSQPEYYTDQIIK 309

Query: 311 ALMLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPFL 370
            LM+V+L AGT+TS  T+EW +S LLNHPE+++KA+ E+DT +G+D  ++E D++KLP+L
Sbjct: 310 GLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYL 369

Query: 371 RGIINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKFD 430
           + I+ ETLR++  APL+VPH SS+D ++GGY+IP+ T+L VN W IH  P +W+ P  F 
Sbjct: 370 QSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFK 429

Query: 431 PDRFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDMT 490
           P+RFE+ G      KL+ FG GRR CPGE L  R  GL LG LIQCF+W+R GE+ +DM 
Sbjct: 430 PERFEKEGEVN---KLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMV 489

Query: 491 EGIAVSMPKARRLQAKCKPR 505
           E   ++  K   L A CK R
Sbjct: 490 EAKGITAGKKTSLNAMCKVR 490

BLAST of Sed0018041 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.6e-126
Identity = 233/504 (46.23%), Postives = 323/504 (64.09%), Query Frame = 0

Query: 14  FFVFYVLTVQFLHKIR--NLPPTPFPSLPIIGHLHLLKKPIYRTLTDISN--RYGPVMFL 73
           FF+F  L + F  + R  NLPP+P    PIIGHLHLLK P++R    +S       +  L
Sbjct: 20  FFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESLNNAKIFSL 79

Query: 74  HFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDHWRNLRR 133
             GSR V VVSS A AEEC +KND+V ANRP  L+ K  GYN+T++V A YGD WRNLRR
Sbjct: 80  SLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWRNLRR 139

Query: 134 ISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGDENQV--HDMNAMLFSLTFNVMLRMIV 193
           I T+E+ S+ RL    ++R DE++ L+  LA+  ++     +M  +   LT N ++RM+ 
Sbjct: 140 IGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTINNIIRMVA 199

Query: 194 GKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMKCHKR 253
           GKRFYGD  E+D+EAK  R+L  E     G  N  D+ P++ ++   +  E  + K   R
Sbjct: 200 GKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV---TNYEKHVKKLAGR 259

Query: 254 RDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTIKALM 313
            D F+Q+L++++R  K+                 TM++ LL LQE++P +Y D  IK ++
Sbjct: 260 VDEFLQSLVNEKRVEKVKG--------------NTMIDHLLSLQETQPDYYTDVIIKGII 319

Query: 314 LVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPFLRGI 373
           LV++ AGT+TS  T+EW +S LLNHPEVL+KA+ EID ++G D L+ E D+ KLP+L+ I
Sbjct: 320 LVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHI 379

Query: 374 INETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKFDPDR 433
           ++ETLR+YP AP+++PH +S+DC V GY +PRGT++ VN WAIH  P +W  P KF P+R
Sbjct: 380 VSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPER 439

Query: 434 FERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDMTEG- 493
           FE+ G +K   KLMPFG GRR CPG GL  R++ L LGSL+QCF+WER  E  +DM E  
Sbjct: 440 FEKKGEDK---KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESE 499

Query: 494 IAVSMPKARRLQAKCKPRSILDRV 511
              +M KA  LQA CK R I+ +V
Sbjct: 500 KGTTMRKATSLQAMCKARPIVHKV 503

BLAST of Sed0018041 vs. ExPASy TrEMBL
Match: A0A6J1FJC9 (cytochrome P450 81D1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445994 PE=3 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 2.7e-241
Identity = 411/515 (79.81%), Postives = 460/515 (89.32%), Query Frame = 0

Query: 4   LGAICFYIPAFFVFYVLTVQFLHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDISNRY 63
           +GAI  YIP FFV YVLT QFLH+IRNLPPTPF SLPIIGHLHLLKKP+YRTL +ISNRY
Sbjct: 1   MGAIIVYIPLFFVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 64  GPVMFLHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDH 123
           GPV+FL FGSR VLVVSSP+AAEECLSKND+VFA+RPRLL+SKWFGYNN +LVW+ YGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 124 WRNLRRISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGDENQVHDMNAMLFSLTFNVML 183
           WRNLRRISTVE+LSTHRLQML+AVRLDEVKSL+ RLA+G ENQV DMN   F LTFNVML
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRLDEVKSLIGRLAKG-ENQVQDMNNAFFDLTFNVML 180

Query: 184 RMIVGKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMK 243
           RMIVGKRFYG+DVED DEA RFRELQ++STQL GK NM DFIPLMSWLGLG   E +IM+
Sbjct: 181 RMIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGWAAEKEIME 240

Query: 244 CHKRRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTI 303
           CH++RDAFMQNLI+Q + R ++ E   +   FRDG KKT++EVLLELQES+P  YND+TI
Sbjct: 241 CHQKRDAFMQNLINQHKNRAIETEPDNS---FRDGRKKTLIEVLLELQESDPDQYNDETI 300

Query: 304 KALMLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPF 363
           +ALMLVLLAAGTETS+NTMEW LSL+LN+PEVLQKAQLEID KVG+D LM+ESD+A +PF
Sbjct: 301 RALMLVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPF 360

Query: 364 LRGIINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKF 423
           LRGIINETLRMYPPAPLMVPHESSKDC+VGGYHIPRGTML+VNLWAIHN P IWA PRKF
Sbjct: 361 LRGIINETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKF 420

Query: 424 DPDRFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDM 483
           DPDRF ++  EK GFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCF+WERPGE+LVDM
Sbjct: 421 DPDRFVKVESEKLGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEELVDM 480

Query: 484 TEGIAVSMPKARRLQAKCKPRSILDRVDLSQTQHN 519
           TEG+AVSMPKAR LQAKCKPR I+DR+ LSQT+ N
Sbjct: 481 TEGVAVSMPKARHLQAKCKPRPIVDRLHLSQTKSN 511

BLAST of Sed0018041 vs. ExPASy TrEMBL
Match: A0A6J1J228 (cytochrome P450 81D1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481119 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 4.6e-241
Identity = 410/515 (79.61%), Postives = 458/515 (88.93%), Query Frame = 0

Query: 4   LGAICFYIPAFFVFYVLTVQFLHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDISNRY 63
           +GAI  YIP F V YVLT QF+HKIRNLPPTPF SLPIIGHLHLLKKP+YRTL +ISNRY
Sbjct: 1   MGAIILYIPLFLVLYVLTEQFIHKIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 64  GPVMFLHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDH 123
           GPV+FL FGSR VLVVSSP+AAEECLSKND+VFA+RPRLL+SKWFGYNN +LVW+ YGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 124 WRNLRRISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGDENQVHDMNAMLFSLTFNVML 183
           WRNLRRISTVE+LSTHRLQML+ VRLDEVKSL+ RLARG ENQV DMN   F LTFNVML
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAGVRLDEVKSLIGRLARG-ENQVQDMNNAFFDLTFNVML 180

Query: 184 RMIVGKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMK 243
           RMIVGKRFYG+DVED DEA RFRELQ++STQL GK NM DFIPLMSWLGLG   E +IM+
Sbjct: 181 RMIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGRAAEKEIME 240

Query: 244 CHKRRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTI 303
           CH++RDAFMQNLI+Q + R ++ E      +FRDG KKT++EVLLELQES+P  YND+TI
Sbjct: 241 CHQKRDAFMQNLINQHKNRAIETE---PHNLFRDGRKKTLIEVLLELQESDPDQYNDETI 300

Query: 304 KALMLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPF 363
           +ALMLVLLAAGTETS+NTMEW LSL+LN+PEVLQKAQLEID KVG+D LM+ESD+A +PF
Sbjct: 301 RALMLVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPF 360

Query: 364 LRGIINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKF 423
           LRGIINETLRMYPPAPLMVPHESSKDC+VGGYHIPRGTML+VNLWAIHN P IWA PRKF
Sbjct: 361 LRGIINETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKF 420

Query: 424 DPDRFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDM 483
           DPDRF ++  EK GFKL+PFGSGRRGCPGEGLGLRVIGLVLGSLIQCF+WERPGEDLVDM
Sbjct: 421 DPDRFVKVDSEKLGFKLLPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDM 480

Query: 484 TEGIAVSMPKARRLQAKCKPRSILDRVDLSQTQHN 519
           TEG+AVSMPKAR LQAKCKPR I+DR+ LSQT+ N
Sbjct: 481 TEGVAVSMPKARHLQAKCKPRPIVDRLHLSQTKSN 511

BLAST of Sed0018041 vs. ExPASy TrEMBL
Match: A0A6J1CYI5 (cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111015737 PE=3 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 8.2e-222
Identity = 378/522 (72.41%), Postives = 446/522 (85.44%), Query Frame = 0

Query: 1   MRSLGAICFYIPAFFVFYVLTVQFLHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDIS 60
           + ++GAI  Y P F V Y+LT  FLHKIRNLPPTPFPSLP+IGHL+LL KPIYRTL DIS
Sbjct: 6   LAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADIS 65

Query: 61  NRYGPVMFLHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPY 120
           NR+GPV+FL FGSRPVLVVSSP+ AEECL+KND+VFANRPRLL+SK FGYNNT+LVW+ Y
Sbjct: 66  NRHGPVVFLRFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSY 125

Query: 121 GDHWRNLRRISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGD-ENQVHDMNAMLFSLTF 180
           GDHWR+LRRISTVEVLSTHRLQ+LS VRL+EV+SL+R+LA+ + ++++  M    F LTF
Sbjct: 126 GDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTF 185

Query: 181 NVMLRMIVGKRFYGDDVED-DDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLE 240
           NVMLRMI GKRFYGDDV+D D++AK FRELQ EST+L+G  N+ DF+P+M+WLG G ++E
Sbjct: 186 NVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVE 245

Query: 241 NKIMKCHKRRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHY 300
            K+M CH +RDAFMQ LI++QRKR         +  FRDG +KTM+EVLLELQESEP HY
Sbjct: 246 KKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHY 305

Query: 301 NDQTIKALMLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDV 360
            D++IKALMLVLL AGTETSVNTMEW LSLLLNHPEVL+KAQ+EID +VG D L++ESD+
Sbjct: 306 TDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDM 365

Query: 361 AKLPFLRGIINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWA 420
           A LP+LRGII ET RMYPPAPLMVPH+SS+DC+VGG+HIPRGTMLYVNLWAIHN P IWA
Sbjct: 366 AHLPYLRGIIXETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWA 425

Query: 421 HPRKFDPDRFERLGREK--FGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERP 480
           +P KFDPDRF+RL  +K  +GF LMPFGSGRRGCPGEGLGLR+IGLVLGSLIQCF+WERP
Sbjct: 426 NPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERP 485

Query: 481 GEDLVDMTEGIAVSMPKARRLQAKCKPRSILDRVDLSQTQHN 519
           GE LVDMTEGIAVSMPKA+ LQAKC+PR I+  +  SQT+ N
Sbjct: 486 GEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKTN 526

BLAST of Sed0018041 vs. ExPASy TrEMBL
Match: A0A1S3BUR6 (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103493892 PE=3 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 1.2e-220
Identity = 373/519 (71.87%), Postives = 435/519 (83.82%), Query Frame = 0

Query: 1   MRSLGAICFYIPAFFVFYVLTVQFLHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDIS 60
           M  LG+I  Y P F V Y+L    LHKIRNLPPTPFP LPIIGHLHLLKKPIYRTL  IS
Sbjct: 1   MGVLGSIFVYFPLFLVLYILIEHLLHKIRNLPPTPFPLLPIIGHLHLLKKPIYRTLAKIS 60

Query: 61  NRYGPVMFLHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPY 120
           N YGPV++  FGSR VLVVSS  AAEECL+KND+VFANRPRL++SKWFGYNNT+L+W+ Y
Sbjct: 61  NHYGPVVYFRFGSRKVLVVSSSFAAEECLTKNDVVFANRPRLIISKWFGYNNTNLIWSSY 120

Query: 121 GDHWRNLRRISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGDENQVHDMNAMLFSLTFN 180
           GDHWRNLRRIST+E+LSTHRLQM S+VRL+EVKSL+RRLA  DENQ+H+M    F LT+N
Sbjct: 121 GDHWRNLRRISTIEILSTHRLQMFSSVRLEEVKSLIRRLA-NDENQIHNMKNEFFDLTYN 180

Query: 181 VMLRMIVGKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENK 240
           +MLRM+VGKR+YG+DV+D DEAK FR+LQ +  QL GK  ++DFIPL+SW+G GSTLENK
Sbjct: 181 MMLRMLVGKRYYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPLVSWMGFGSTLENK 240

Query: 241 IMKCHKRRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYND 300
           I++ H +RD FMQNLIDQ +KR  D     +  I++DG +KTM+EVLLELQ+ EP  Y D
Sbjct: 241 IIELHVKRDTFMQNLIDQHKKRNFD--QNNSNKIYQDGRRKTMIEVLLELQQYEPNQYTD 300

Query: 301 QTIKALMLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAK 360
           +TI+ALML+LLAAGTETSV TMEW LSL+LNHPE L+K Q EID++VG+D L++ESD+A 
Sbjct: 301 ETIRALMLILLAAGTETSVVTMEWALSLMLNHPEFLKKLQNEIDSQVGHDRLIDESDMAN 360

Query: 361 LPFLRGIINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHP 420
           LP LRGIINET RMYPPAPLMVPHESSKDC VGGYHIPRGT+LYVNLWAIHN P IW +P
Sbjct: 361 LPSLRGIINETFRMYPPAPLMVPHESSKDCIVGGYHIPRGTILYVNLWAIHNDPKIWDNP 420

Query: 421 RKFDPDRFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDL 480
           RKF+P+RFE L  EKFGF LMPFGSGRRGCPGEGLGLRVIGLVLGSL+QCF+WERPGE+L
Sbjct: 421 RKFNPNRFESLESEKFGFNLMPFGSGRRGCPGEGLGLRVIGLVLGSLVQCFEWERPGEEL 480

Query: 481 VDMTEGIAVSMPKARRLQAKCKPRSILDRVDLSQTQHNP 520
           VDMTEGIA++MPKA  LQAKC PR I+ R+ LS    NP
Sbjct: 481 VDMTEGIALTMPKAHCLQAKCTPRPIVHRLHLSHKISNP 516

BLAST of Sed0018041 vs. ExPASy TrEMBL
Match: A0A0A0L2I7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642300 PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.6e-217
Identity = 365/519 (70.33%), Postives = 434/519 (83.62%), Query Frame = 0

Query: 1   MRSLGAICFYIPAFFVFYVLTVQFLHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDIS 60
           M  L +I  YIP FFV Y L    +HKIRNLPPTPFP LP+IGHLHLLK PIYRTL +IS
Sbjct: 1   MGVLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS 60

Query: 61  NRYGPVMFLHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPY 120
           N YGPV++  FG R VLVVSSP AAEECL+KNDIVFANRPRL++SKWFGYNNT+LVW+ Y
Sbjct: 61  NHYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPRLIISKWFGYNNTNLVWSSY 120

Query: 121 GDHWRNLRRISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGDENQVHDMNAMLFSLTFN 180
           GDHWRNLRRIST+E+LSTHR+QMLS  RL+EVKSL+RRLA  +ENQ+H+M    F LT+N
Sbjct: 121 GDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLA-NNENQIHNMKNEFFDLTYN 180

Query: 181 VMLRMIVGKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENK 240
           VMLRM+VGKRFYG+DV+D DEAK FR+LQ +  QL GK  ++DFIP +SW+G GST+ENK
Sbjct: 181 VMLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENK 240

Query: 241 IMKCHKRRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYND 300
           I++CH +RD FMQNLIDQ +KR +D    ++  I +DG +KTM+EVLLELQ+  P  Y D
Sbjct: 241 IIECHVKRDTFMQNLIDQHKKRIVD--QNKSNNISQDGRRKTMIEVLLELQQYNPDQYTD 300

Query: 301 QTIKALMLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAK 360
           +TI+ALMLVLLAAGTETSV  MEW LSL+LNHP+ L+K Q EID +VG+D L++ESD+A 
Sbjct: 301 ETIRALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMAN 360

Query: 361 LPFLRGIINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHP 420
           LP LRGIINETLRMYPPAPL+VPHESSKDCT+GGYHIPRGT+L+VNLWAIHN P IW +P
Sbjct: 361 LPSLRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNP 420

Query: 421 RKFDPDRFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDL 480
           RKF+P+RFE L  EKFGF L+PFGSGRRGCPGEGL LRVIGLVLG+L+QCF+WERPGE+L
Sbjct: 421 RKFNPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEEL 480

Query: 481 VDMTEGIAVSMPKARRLQAKCKPRSILDRVDLSQTQHNP 520
           VDMTEG+A++MPKA  LQAKC PR I+ R+ LS  + NP
Sbjct: 481 VDMTEGVALTMPKAHCLQAKCTPRPIVHRLHLSHKESNP 516

BLAST of Sed0018041 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 471.5 bits (1212), Expect = 9.2e-133
Identity = 246/504 (48.81%), Postives = 337/504 (66.87%), Query Frame = 0

Query: 16  VFYVLTVQFLH-KIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDIS-----NRYGPVMFL 75
           +F +++ +FL  K +NLPP+P   LPIIGHL LLK PI+RTL   S     N  G VM L
Sbjct: 17  IFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDGGGVMSL 76

Query: 76  HFGSRPVLVVSS-PAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDHWRNLR 135
             GSR V VVSS   AAEEC  KND+V ANRP++++ K  GYNNT+++ APYGDHWRNLR
Sbjct: 77  RLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHWRNLR 136

Query: 136 RISTVEVLSTHRLQMLSAVRLDEVKSLLRRLAR--GDENQVHDMNAMLFSLTFNVMLRMI 195
           R+ T+E+ STHRL     VR DEV+ L+ RL+R  G +  V ++  ML  LTFN ++RM+
Sbjct: 137 RLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTFNNIMRMM 196

Query: 196 VGKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMKCHK 255
            GKR+YG++  D++EAKR R+L  +    +   N  D++P+   L L S+ EN++ K  +
Sbjct: 197 TGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPI---LRLFSSYENRVKKLGE 256

Query: 256 RRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTIKAL 315
             D F+Q LID +R ++   ETG            TM++ LL LQ+S+ ++Y DQ IK +
Sbjct: 257 ETDKFLQGLIDDKRGQQ---ETG-----------TTMIDHLLVLQKSDIEYYTDQIIKGI 316

Query: 316 MLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPFLRG 375
           +L+++ AGT TS  T+EW LS LLNHP+V+ KA+ EID +VG D L+ E+D+++LP+L+ 
Sbjct: 317 ILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKN 376

Query: 376 IINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKFDPD 435
           I+ ETLR++P  PL+VPH +S+DC +G Y +PRGT L VN WAIH  P  W  P  F P+
Sbjct: 377 IVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPE 436

Query: 436 RFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDMTEG 495
           RFE   +E+   KL+ FG GRR CPG GL  R++GL LGSLIQCF+WER G   VDM EG
Sbjct: 437 RFE---KEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEG 496

Query: 496 IAVSMPKARRLQAKCKPRSILDRV 511
           +  ++PKA  L+A CK R  L ++
Sbjct: 497 VGNTVPKAIPLKAICKARPFLHKI 500

BLAST of Sed0018041 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 470.3 bits (1209), Expect = 2.0e-132
Identity = 240/504 (47.62%), Postives = 329/504 (65.28%), Query Frame = 0

Query: 15  FVFYVLTVQFL----HKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDISNRYG--PVMF 74
           F F VL++ FL     +  NLPP+P  +LP+IGHL LLK P++R    +S   G  P++ 
Sbjct: 10  FCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPIIS 69

Query: 75  LHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDHWRNLR 134
           L  G+R + VVSS + AEEC +KND++ ANR   + +K   Y N+++V A Y +HWRNLR
Sbjct: 70  LRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRNLR 129

Query: 135 RISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGDENQVH--DMNAMLFSLTFNVMLRMI 194
           RI  +E+ S HRL   S++R DE++ L+ RL R         +M +M   LTFN ++RM+
Sbjct: 130 RIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNIIRML 189

Query: 195 VGKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMKCHK 254
            GK +YGD  EDD EAKR R L  E+   SG  N  D+IP+++W+   +  E +I K   
Sbjct: 190 AGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWI---TYSETRIKKLAG 249

Query: 255 RRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTIKAL 314
           R D F+Q L+D++R+ K   E              TM++ LL LQE++P++Y D+ IK  
Sbjct: 250 RLDEFLQGLVDEKREGKEKKE-------------NTMVDHLLCLQETQPEYYMDRIIKGT 309

Query: 315 MLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPFLRG 374
           ML L+A GT+T+  T+EW LS LLN+PEVL KA+ EID  +G D L+ ESD+  LP+L+ 
Sbjct: 310 MLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQN 369

Query: 375 IINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKFDPD 434
           I++ETLR+YP AP+++PH +SKDC VGGY +PRGTML  N WAIH  P +W  P  F P+
Sbjct: 370 IVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPE 429

Query: 435 RFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDMTEG 494
           RFE+ G  K   KLMPFG GRR CPG GL  R++ L LGSLIQCF+WER GE+ VDMTEG
Sbjct: 430 RFEKEGEAK---KLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEG 489

Query: 495 IAVSMPKARRLQAKCKPRSILDRV 511
             ++MPKAR L+A C+ R  + ++
Sbjct: 490 PGLTMPKARPLEAMCRARDFVGKI 494

BLAST of Sed0018041 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 469.2 bits (1206), Expect = 4.5e-132
Identity = 245/497 (49.30%), Postives = 332/497 (66.80%), Query Frame = 0

Query: 16  VFYVLTVQF----LHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDISN--RYGPVMFL 75
           +F VL++ +    L +  NLPP+P  SLP+IGHL LLK PI+RT   +S      P+  L
Sbjct: 11  LFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIFSL 70

Query: 76  HFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDHWRNLRR 135
             G+R V V SS + AEEC +KND+V ANRP  +L+K   Y+ T+++ A YGDHWRNLRR
Sbjct: 71  RLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNLRR 130

Query: 136 ISTVEVLSTHRLQMLSAVRLDEVKSLLRRLAR--GDENQVHDMNAMLFSLTFNVMLRMIV 195
           I +VE+ S HRL    ++R DE++ L+ RL+R    E    DM +ML  LTFN +LRM+ 
Sbjct: 131 IGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRMVA 190

Query: 196 GKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMKCHKR 255
           GKR+YGD VEDD EAKR R+L  +    +G  N  D++P+   L L S  E ++ K   R
Sbjct: 191 GKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPV---LRLVSDYETRVKKLAGR 250

Query: 256 RDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTIKALM 315
            D F+Q L+D++R+ K   E G            TM++ LL LQES+P ++ D+ IK  M
Sbjct: 251 LDEFLQGLVDEKREAK---EKG-----------NTMIDHLLTLQESQPDYFTDRIIKGNM 310

Query: 316 LVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPFLRGI 375
           L L+ AGT+TS  T+EW LS +LNHP+VL KA+ EID K+G D LM+ESD++ LP+L+ I
Sbjct: 311 LALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNI 370

Query: 376 INETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKFDPDR 435
           ++ETLR+YP AP+++PH +S+DC V GY +PRGT+L  N+WAIH  P +W  P  F P+R
Sbjct: 371 VSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPER 430

Query: 436 FERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDMTEGI 495
           FE+ G  +   KLMPFG GRR CPG GL  R+I L LGSLIQC +WE+ GE+ VDM+EG 
Sbjct: 431 FEKEGEAQ---KLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGK 486

Query: 496 AVSMPKARRLQAKCKPR 505
            V+MPKA+ L+A C+ R
Sbjct: 491 GVTMPKAKPLEAMCRAR 486

BLAST of Sed0018041 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 464.2 bits (1193), Expect = 1.5e-130
Identity = 230/506 (45.45%), Postives = 337/506 (66.60%), Query Frame = 0

Query: 15  FVFYVLTVQFL----HKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDISNRYG--PVMF 74
           F+F  ++++ L    ++  NLPP+P   LP+IGHLHLLK+P++RT   IS   G  P+  
Sbjct: 11  FLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPIFH 70

Query: 75  LHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDHWRNLR 134
           L  G+R V V+SS + AEEC +KND+V ANRP ++++K  GYN T+++ A YGDHWRNLR
Sbjct: 71  LRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRNLR 130

Query: 135 RISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGDENQVH-----DMNAMLFSLTFNVML 194
           RI+ VE+ S+HR+   S++R DE++ L+  L+R   + +H     ++ ++L +L FN ++
Sbjct: 131 RIAAVEIFSSHRISTFSSIRKDEIRRLITHLSR---DSLHGFVEVELKSLLTNLAFNNII 190

Query: 195 RMIVGKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMK 254
            M+ GKR+YG   ED+DEAK  REL  E    +G  N+ D++P ++W+   +  EN+   
Sbjct: 191 MMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV---TNFENQTKI 250

Query: 255 CHKRRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTI 314
              R D  +Q L+D++R  K   E G           +T+++ LL  QE+EP++Y D  I
Sbjct: 251 LGNRLDRVLQKLVDEKRAEK---EKG-----------QTLIDHLLSFQETEPEYYTDVII 310

Query: 315 KALMLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPF 374
           K ++L L+ AGT+TS  T+EW +S LLNHPE+L+KA+ EID K+G+D L+ ESD+  L +
Sbjct: 311 KGIILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHY 370

Query: 375 LRGIINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKF 434
           L+ I++ETLR+YP  PL++PH SS +C V GY +PR T+L  N+WA+H  P +W  P +F
Sbjct: 371 LQNIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERF 430

Query: 435 DPDRFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDM 494
            P+RFE+ G  +   KLMPFG GRR CPG  LG R++ L LG LIQ F+WER G +LVDM
Sbjct: 431 KPERFEKEGEAR---KLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDM 490

Query: 495 TEGIAVSMPKARRLQAKCKPRSILDR 510
           TEG  ++MPKA  L+A CK R+I+ +
Sbjct: 491 TEGEGITMPKATPLRAMCKARAIVGK 493

BLAST of Sed0018041 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 463.4 bits (1191), Expect = 2.5e-130
Identity = 244/505 (48.32%), Postives = 330/505 (65.35%), Query Frame = 0

Query: 15  FVFYVLTVQF----LHKIRNLPPTPFPSLPIIGHLHLLKKPIYRTLTDISNRYG--PVMF 74
           F+F  L++ F    + +  NLPP+P  +LP+IGHL LLK P++R    +S   G  P++ 
Sbjct: 10  FLFISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPIIS 69

Query: 75  LHFGSRPVLVVSSPAAAEECLSKNDIVFANRPRLLLSKWFGYNNTSLVWAPYGDHWRNLR 134
           L  G+R V VVSS + AEEC +KND+V ANR   L SK   Y  T++V A YGDHWRNLR
Sbjct: 70  LRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNLR 129

Query: 135 RISTVEVLSTHRLQMLSAVRLDEVKSLLRRLARGD--ENQVHDMNAMLFSLTFNVMLRMI 194
           RI  VE+ S HRL   S++R DE+  L+  L+R    E    +M +M  +LTFN ++RM+
Sbjct: 130 RIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNIIRML 189

Query: 195 VGKRFYGDDVEDDDEAKRFRELQTESTQLSGKPNMRDFIPLMSWLGLGSTLENKIMKCHK 254
            GK +YGD  EDD EAKR REL  E     G  N  D++P+++W+  GS  E +I K   
Sbjct: 190 AGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI-TGS--EKRIKKIAS 249

Query: 255 RRDAFMQNLIDQQRKRKMDAETGRAEYIFRDGVKKTMMEVLLELQESEPQHYNDQTIKAL 314
           R D F+Q L+D++R+ K             +  + TM++ LL LQE++P++Y D  IK +
Sbjct: 250 RLDEFLQGLVDERREGK-------------EKRQNTMVDHLLCLQETQPEYYTDNIIKGI 309

Query: 315 MLVLLAAGTETSVNTMEWGLSLLLNHPEVLQKAQLEIDTKVGNDHLMNESDVAKLPFLRG 374
           ML L+ AGT+TS  T+EW LS LLNHP++L KA+ EID KVG + L+ ESD++ LP+L+ 
Sbjct: 310 MLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQN 369

Query: 375 IINETLRMYPPAPLMVPHESSKDCTVGGYHIPRGTMLYVNLWAIHNHPTIWAHPRKFDPD 434
           I++E+LR+YP +PL+VPH +S+DC VGGYH+PRGTML  N WAIH  P IW  P  F P+
Sbjct: 370 IVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPE 429

Query: 435 RFERLGREKFGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFQWERPGEDLVDMTE- 494
           RFE+ G  +   KL+ FG GRR CPG GL  R+  L +GSLIQCF+WER GE+ VDMTE 
Sbjct: 430 RFEKEGEAQ---KLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEG 489

Query: 495 GIAVSMPKARRLQAKCKPRSILDRV 511
           G  V MPKA  L A CK R ++ ++
Sbjct: 490 GGGVIMPKAIPLVAMCKARPVVGKI 495

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022940352.15.6e-24179.81cytochrome P450 81D1-like isoform X1 [Cucurbita moschata][more]
XP_022982225.19.5e-24179.61cytochrome P450 81D1-like isoform X1 [Cucurbita maxima][more]
XP_023523281.12.0e-23879.22cytochrome P450 81D1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022146589.11.7e-22172.41cytochrome P450 81D1-like [Momordica charantia][more]
XP_008453068.12.4e-22071.87PREDICTED: cytochrome P450 81D1-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
W8JMU72.7e-13751.24Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
Q9FG651.3e-13148.81Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1[more]
P931471.6e-12645.45Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2[more]
Q6WNQ81.6e-12646.60Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1[more]
Q9LHA11.6e-12646.23Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FJC92.7e-24179.81cytochrome P450 81D1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114459... [more]
A0A6J1J2284.6e-24179.61cytochrome P450 81D1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481119... [more]
A0A6J1CYI58.2e-22272.41cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111015737 PE=3 SV... [more]
A0A1S3BUR61.2e-22071.87cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103493892 PE=3 SV=1[more]
A0A0A0L2I71.6e-21770.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642300 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G36220.19.2e-13348.81cytochrome p450 81d1 [more]
AT4G37360.12.0e-13247.62cytochrome P450, family 81, subfamily D, polypeptide 2 [more]
AT4G37370.14.5e-13249.30cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37320.11.5e-13045.45cytochrome P450, family 81, subfamily D, polypeptide 5 [more]
AT4G37340.12.5e-13048.32cytochrome P450, family 81, subfamily D, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 302..319
score: 31.68
coord: 84..105
score: 27.69
coord: 322..348
score: 40.37
coord: 406..430
score: 33.52
coord: 365..383
score: 32.64
coord: 60..79
score: 39.94
coord: 440..450
score: 55.41
coord: 450..473
score: 35.42
coord: 177..195
score: 29.49
IPR001128Cytochrome P450PRINTSPR00385P450coord: 313..330
score: 37.17
coord: 366..377
score: 40.67
coord: 441..450
score: 57.38
coord: 450..461
score: 31.36
IPR001128Cytochrome P450PFAMPF00067p450coord: 33..493
e-value: 8.6E-94
score: 314.9
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 26..513
e-value: 9.9E-125
score: 418.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 32..504
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 24..472
e-value: 2.5E-19
score: 67.4
NoneNo IPR availablePANTHERPTHR47947CYTOCHROME P450 82C3-RELATEDcoord: 15..508
NoneNo IPR availablePANTHERPTHR47947:SF24CYTOCHROME P450 81E8coord: 15..508
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 443..452

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0018041.1Sed0018041.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098542 defense response to other organism
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen